CN108849901A - It is a kind of for preventing and treating the biological pesticide preparation and its preparation and application of the late blight of potato - Google Patents
It is a kind of for preventing and treating the biological pesticide preparation and its preparation and application of the late blight of potato Download PDFInfo
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- CN108849901A CN108849901A CN201810937191.2A CN201810937191A CN108849901A CN 108849901 A CN108849901 A CN 108849901A CN 201810937191 A CN201810937191 A CN 201810937191A CN 108849901 A CN108849901 A CN 108849901A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
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Abstract
The present invention provides a kind of for preventing and treating the biological pesticide preparation of the late blight of potato, including following materials by weight：Amino-oligosaccharide 1-5%, carvacrol 2-10%, solvent complement to 100%, and preparation method is：Carvacrol is dissolved with edible alcohol, is A liquid；Amino-oligosaccharide is dissolved with acetum, is B liquid；A liquid and B liquid and sophorolipid are added in distilled water, high speed shear 15-25 minutes, are completely dispersed mixture uniformly to get product biological pesticide preparation.In pesticidal preparations of the present invention, the stronger synergistic effect of phase mutual between amino-oligosaccharide and carvacrol, the immunoregulation effect of amino-oligosaccharide and the germ killing effect of carvacrol are mutually complementary, control efficiency is significant, and it can be improved yield, environmentally friendly noresidue is the ideal substitute of chemical pesticide in Modern Green ecological agriculture development.
Description
Technical field
The invention belongs to technical field of pesticide, be related to a kind of biological pesticide preparation for preventing and treating the late blight of potato and its
Preparation and application.
Background technique
The late blight of potato（Potato Late Blight）To be caused by phytophthora infestans, cause potato haulm death and
A kind of crushing oomycetes disease that stem tuber rots.All over the world, there are generation, popular year in potato producing region to the late blight of potato
The general underproduction 30%.The Irish potato mortality forties in 19th century, underproduction half keep more than 100 ten thousand people hungry to death, and 2,000,000
People's resettlement.There are various suppositions to the reason of potato death at that time, it is that germ is drawn that Feng Madiyousi in 1842, which is first considered that,
It rises, this Pierre in 1857 is permitted how moral proves that mould can cause stem tuber to rot on leaf.Moral Bali has determined on leaf within 1861~1863 years
Scab and stem tuber rot to be caused by a kind of fungi and to identifying pathogen.There is generation in the potatoes place of production, west
Southern area is more serious, northeast, rainy humidity in North China and northwest time cause harm it is heavier, if nineteen fifty is very popular year, these
Area's loss 30%~50%.Having 5 years in later 10 years again is popular year.During late blight of potato prevention and treatment, using tradition
Chemical pesticide be easy to form medicament residue in potato epidermis, have to human body and certain poison risk.Food safety increasingly
Under concerned overall situation, the biological pesticide preparation for developing a kind of safety and environmental protection seems especially urgent.
Amino-oligosaccharide, English name are:Oligosaccharins, also referred to as agriculture dedicated chitosan oligosaccharide, be chitin,
The upgrading products of chitosan product, with the incomparable superiority of chitosan, have it is nontoxic, dissolve in slightly acidic water solution
In, there is good film forming and biodegradability, gas selective penetrated property and antibiotic property, be that a kind of edible natural is fresh-keeping
Material is widely used in the research of preserving fruit and vegetable utilizing.
Carvacrol, English name are：Carvacrol is the volatile oil extracted from natural plants wild marjoram, is mainly used for preparing fragrant
Material, fungicide and disinfectant, also serve as edible essence.Carvacrol has had relevant report, Ru Anyong as fungicide, antistaling agent
It learns, what Dong Fang et al. was delivered《Control efficiency of 5% carvacrol to pepper powdery mildew》Confirm that botanical pesticide carvacrol is used for
Pepper powdery mildew is prevented and treated, can be reduced the usage amount of chemical pesticide, Pesticide Residue is solved, improves the quality and yield of capsicum.
Number of patent application is 201410477987.6《A kind of cinnamic acid and carvacrol natural built-up preservative》Application for a patent for invention,
Confirm that carvacrol has the function of sterilization, antibacterial, fresh-keeping.
In agriculture field, each effective component is mutually mixed, and synergy is good with the prevention and treatment effect that can effectively improve pest and disease damage
Fruit reduces the amount of application of medicament.In terms of preventing and treating the late blight of potato, made by improving immunoregulation effect and killing fungi
With can preferably prevent and treat the generation of the late blight of potato.Currently, preventing and treating horse using amino-oligosaccharide and carvacrol as effective component
Bell potato late blight yet there are no relevant report.
Summary of the invention
It is anti-it is an object of the invention in view of the problems of the existing technology, provide a kind of late blight of potato of nonhazardous
It controls and uses biological pesticide preparation.
Another object of the present invention is to provide a kind of preparation methods of above-mentioned biological pesticide preparation.
A further object of the present invention is to provide a kind of application methods of above-mentioned biological pesticide preparation.
For this purpose, the present invention adopts the following technical scheme that：
It is a kind of for preventing and treating the biological pesticide preparation of the late blight of potato, including following materials by weight：Amino
Oligosaccharide 1-5%, carvacrol 2-10%, solvent complement to 100%.
Further, the solvent includes edible alcohol and distilled water.
Further, the solvent further includes acetum.
Further, the raw material components further include the sophorolipid of 0.5-2%.It is living that sophorolipid is used as surface in the present invention
Property agent, have Nantural non-toxic, the good feature of Environmental compatibility.
Further, the weight percent of the edible alcohol is 8-12%, and distilled water complements to 100%.
Further, the concentration expressed in percentage by volume of the acetum is 0.5-2%, weight of the acetum in raw material components
Amount percentage is 5-10%.
A kind of above-mentioned for preventing and treating the preparation method of the biological pesticide preparation of the late blight of potato, detailed process is as follows：
It requires to weigh carvacrol and amino-oligosaccharide by weight ratio, carvacrol is dissolved with edible alcohol, be A liquid；By amino
Oligosaccharide is dissolved with acetum, is B liquid；A liquid and B liquid and sophorolipid are added in distilled water, high speed shear 15-25 minutes,
It is completely dispersed mixture uniformly to get product biological pesticide preparation.
It is a kind of above-mentioned for preventing and treating the application method of the biological pesticide preparation of the late blight of potato, it is that preparation is diluted with water
Potato leaf surface sprinkling is carried out after 400-1000 times, or is planted after being impregnated seed with above-mentioned dilution in potato planting.
The beneficial effects of the present invention are：
（1）Mutual compatibility between effective component, wherein there is amino-oligosaccharide plant immune to prevent and treat inducing action, improve plant pair
The immunoprophylaxis of late blight, carvacrol have the function of very strong killing plant pathogenic fungi, destroy pathogen cell membrane, inhibit
The synthesis of ergosterol；
（2）The stronger synergistic effect of phase mutual between amino-oligosaccharide and carvacrol, the immunoregulation effect of amino-oligosaccharide and
The germ killing effect of carvacrol is mutually complementary, and preparation of having mutually promoted is to the preventive and therapeutic effect of the late blight of potato；
（3）For effective component from natural drug, safe and nontoxic, prevention and bacteriostasis are obvious, and potato late blight can be effectively reduced
The disease incidence of disease improves the quality of potato, eliminates environmental pollution caused by chemical pesticide, the especially green organic production of said preparation
The optimal selection of product production.
Specific embodiment
Technical solution of the present invention and beneficial effect are described further with reference to embodiments, of the present invention hundred
Ratio is divided to be weight percentage.
Embodiment 1
It is a kind of for preventing and treating the biological pesticide preparation of the late blight of potato, raw material group becomes（By weight percentage）：
Amino-oligosaccharide 2%, carvacrol 5%, sophorolipid 1.0%, edible alcohol 10%, acetum 6%, distilled water complements to
100%.
Wherein, the alcohol content of edible alcohol is 95%vol（Similarly hereinafter）, the concentration expressed in percentage by volume of acetum is 1%.
Preparation method：It requires to weigh carvacrol and amino-oligosaccharide by weight ratio, carvacrol is dissolved with edible alcohol,
For A liquid；Amino-oligosaccharide is dissolved with acetum, is B liquid；A liquid and B liquid and sophorolipid are added in distilled water, cut at a high speed
It cuts 15-25 minutes, is completely dispersed mixture uniformly to get product biological pesticide preparation.
Application method：Potato leaf surface sprinkling is carried out after preparation is diluted with water 400-1000 times, or in potato planting
When seed is impregnated with above-mentioned dilution after plant.
Embodiment 2
It is a kind of for preventing and treating the biological pesticide preparation of the late blight of potato, raw material group becomes（By weight percentage）：
Amino-oligosaccharide 1.0%, carvacrol 3%, sophorolipid 0.5%, edible alcohol 8%, acetum 5%, distilled water complements to
100%；
Wherein, the concentration expressed in percentage by volume of acetum is 2%.
Preparation method and application method are the same as embodiment 1.
Embodiment 3
It is a kind of for preventing and treating the biological pesticide preparation of the late blight of potato, raw material group becomes（By weight percentage）：
Amino-oligosaccharide 3%, carvacrol 2%, sophorolipid 1.5%, edible alcohol 12%, acetum 10%, distilled water complements to
100%；
Wherein, the concentration expressed in percentage by volume of acetum is 2%.
Preparation method and application method are the same as embodiment 1.
Embodiment 4
It is a kind of for preventing and treating the biological pesticide preparation of the late blight of potato, raw material group becomes（By weight percentage）：
Amino-oligosaccharide 1.6%, carvacrol 6%, sophorolipid 2%, edible alcohol 10%, acetum 8%, distilled water complements to
100%；
Wherein, the concentration expressed in percentage by volume of acetum is 1.5%.
Preparation method and application method are the same as embodiment 1.
Embodiment 5
It is a kind of for preventing and treating the biological pesticide preparation of the late blight of potato, raw material group becomes（By weight percentage）：
Amino-oligosaccharide 5%, carvacrol 10%, sophorolipid 1.0%, edible alcohol 10%, acetum 10%, distilled water is supplied
To 100%；
Wherein, the concentration expressed in percentage by volume of acetum is 0.5%.
Preparation method and application method are the same as embodiment 1.
One, the virulence experiment of biological pesticide preparation Phytophthora infestans of the present invention
The virulence of biological pesticide preparation Phytophthora infestans of the present invention is carried out by taking 2 biological pesticide preparation of embodiment as an example below
Effect experiment.
1. germ selects
Phytophthora infestans（Phytophthora infestans）Gansu Province Dingxi City is picked up from, by Gansu Agriculture University's grass cultivation
The separation of institute's pesticide laboratory, and carry out biological test experiment.
The setting of reagent agent concentration is as shown in table 1：
The setting of 1 reagent agent concentration of table
| Number | Processing | Extension rate multiple | Concentration（μg/mL） | Volume is added（μL） |
| 1 | Clear water compares CK | - | - | - |
| 2 | Chemicals treatment | 500 | 200 | 200 |
| 3 | Chemicals treatment | 1000 | 100 | 100 |
| 4 | Chemicals treatment | 2000 | 50 | 50 |
| 5 | Chemicals treatment | 4000 | 25 | 25 |
| 6 | Chemicals treatment | 8000 | 12.5 | 12.5 |
2. experimental method
The preparation of 2.1 rye culture mediums：50 g of rye seed is weighed to impregnate in 1000 mL distilled water after 24 h at 121 DEG C
30 min of high pressure steam sterilization removes slag through 4 layers of filtered through gauze；Supernatant moisturizing is added 20 g of agar and boils, divide to 1000 mL
Loaded in 150 mL triangular flasks, every bottle of packing 100 mL are spare after high pressure sterilization at 121 DEG C.
The preparation of 2.2 pastille culture mediums：When culture medium subject to sterilization is cooled to 50-55 °C, volume shown in numbering is handled by table 1
Reagent agent is sequentially added, after sufficiently shaking up, 20 mL pastille culture mediums is measured with 50 mL graduated cylinders and is placed in culture dish, carry out medicine
Agent and concentration markers.Blank control group is done with clear water, each processing is repeated 3 times, spare after culture medium solidification.
2.3 inoculation pathogens：Aseptically, shift to an earlier date the horse activated on no medicine culture medium with sterilized punch
Bell potato late disease bacteria colony edge takes fungus block（For colony edge strain than comparatively fresh, viability is strong, and more consistent, colony diameter
For 5 mm）；Fungus block is accessed to the pastille culture medium of solidification center prepared by step 2.2, the upper table of fungus block with transfer needle or tweezers
Down；The culture dish for connecting bacterium is placed in 20 °C of insulating boxs and is cultivated.
After the bacterium colony in blank control group close to after covering with culture dish, the straight of each processing bacterium colony is measured with crossing method
Diameter finds out each concentration of medicament to the inhibiting rate of germ with following formula.The bacterium colony growth inhibition ratio of each concentration is converted into probability
Value, concentration are converted into logarithm.Then linear regression equation, EC are found out with log concentration-inhibition percentage probit value50Value.
3. result and analysis
2 bacteriostasis rate result of table
The The Toxicity Determination result of 2 pesticidal preparations Phytophthora infestans of embodiment is as shown in table 2.From table 2 it can be seen that
The pesticidal preparations Phytophthora infestans has stronger activity inhibition, on the rye culture medium of drug containing, each dosage
The phytophthora infestans growth of processing is suppressed, and inhibiting rate is increased with the increase of drug concentration, drug concentration with
Inhibiting effect is positively correlated, regression equation Y=1.421X+2.646.The EC of the pesticidal preparations Phytophthora infestans50Concentration value
For 45.35 μ g/mL.
Two, 2 biological pesticide preparation of embodiment tests the field late blight of potato
1. test material
Mandipropamid（Switzerland-Syngenta China Investment Ltd.）；Fluoxastrobin（Britain-Syngenta China limited investment is public
Department）；Alkene acyl pyrazoles ester（Germany-Basf China Co., Ltd）；60% the third gloomy cymoxanils（Shanghai favour luminous environment science and technology has
Limit company）；50% MnZn flumorph wettable powder（State's light three is controlled, Shenyang Sciencreat Chemicals Co., Ltd.）；Embodiment 2 contains
The biological pesticide preparation of 4% amino-oligosaccharide and carvacrol（Lanzhou Shichuang Biotechnology Co., Ltd.）；58% state's light first frost
MnZn（State's light, Sichuan Guoguang Agrochemical Co., Ltd.）；58% Jiangsu first frost MnZn (precious big gloomy, Jiangsu treasured etherealize work stock
Part Co., Ltd).
2. experimental design and method：
Test site selects the Lintao County in one of Gansu Province mulch potato planting major production areas, specifically in Lintao County gantry town
In three villages Shi Lipu arable land, which is 1930m, and annual rainfall 410.5ml, frost-free period 146d, soil fertility is uniform, and soil is more fertile
Fertile, test variety is local mulch potato Main Cultivars --- Zhuangshu 3, using the full plastic film mulching cultivation of ridge culture, kind
4500 plants/acre of density in planting or so, other cultivation techniques etc. are identical as locality.
Test uses single factor test RANDOMIZED BLOCK DESIGN, if 8 processing groups, every group of 3 cells amount to 24 cells.Plot area
For 2.1m × 14.3m=30m2。
3000~4000kg/ mus of farmyard manure are applied before sowing, applying quantity of chemical fertilizer is unified for 25kg/ mus of urea, calcium superphosphate
50kg/ mus, 15kg/ mus of potassium sulfate, 3kg/ mus of zinc sulfate.Whole phosphate fertilizer and nitrogenous fertilizer are weighed by cell, are once applied before broadcasting by base manure
Enter.
3. test process
In potato seedling stage, by following pharmaceutical quantities, throughout reason group cell internal spraying is primary respectively, sprays again every 7~10 days once,
Even spray 3 times：
Processing group 1：40g/ mus of mandipropamid suspending agent；
Processing group 2：40g/ mus of Fluoxastrobin suspending agent；
Processing group 3：18.7% 100g/ mus of ester water dispersible granules of alkene acyl pyrazoles；
Processing group 4：60% the third 80g/ mus of gloomy cymoxanil wettable powders；
Processing group 5：50% 100g/ mus of MnZn flumorph wettable powder；
Processing group 6：100g/ mus of 2 biological pesticide preparation of embodiment；
Processing group 7：- 58% 100g/ mus of first frost mancozeb wettable powder of Sichuan state light；
Processing group 8：Jiangsu is precious gloomy 100g/ mus of -58% first frost mancozeb wettable powder big（As a control group）；
Processing group 9：Blank control group（CK）, any medicament is not sprayed.
4. calculation formula
Late blight of potato grade scale:
0 grade：Disease-free spot
1 grade：Lesion area accounts for entire 5% or less leaf area；
3 grades：Lesion area accounts for entire leaf area 6%-10%；
5 grades：Lesion area accounts for entire leaf area 11%-20%；
7 grades：Lesion area accounts for entire leaf area 21%-50%；
9 grades：Lesion area accounts for entire 50% or more leaf area.
Wherein：
CK0To be administered preceding check plot disease index；
PT0To be administered pre-treatment area disease index；
CK1For check plot disease index after application；
PT1To be administered post processing zone disease index.
5 interpretations of result
The analysis of 5.1 protection effects：
The effect of statistics prevention and treatment and second of prevention and treatment for the first time respectively, and average preventive effect is calculated, result is included in table 3.
From table 3 it can be seen that -58% 100g/ mus of first frost mancozeb wettable powder of Sichuan state light is averaged twice, preventive effect is
71.64%, occupy first；Averagely preventive effect is 70.27% to 100g/ mus of 2 biological pesticide preparation of embodiment twice, occupies second；Secondly
For 50% MnZn flumorph wettable powder, the 60% the third 80g/ mus of gloomy cymoxanil wettable powders, 18.7% alkene acyl pyrazoles ester
100g/ mus of water dispersible granules, Fluoxastrobin suspending agent, mandipropamid suspending agent, the preventive effect of above-mentioned preparation is respectively 69.15%,
66.61%,66.26%,65.79%,62.73%；And the preventive effect that Jiangsu produces 58% 100g/ mus of first frost mancozeb wettable powder is minimum,
Only 60.69%.
Record analysis of results table in 3 late blight of potato field of table
The analysis of 5.2 yield results
From table 4, it can be seen that several chemicals treatments all have apparent the effect of protective yield to potato, increase production in 3.5-16.63%.Its
- 58% 100g/ mus of spraying yield of first frost mancozeb wettable powder of middle Sichuan state light are 3180.2kg/ mus, compare and increase than clear water
16.63% is produced, big gloomy -58% first frost mancozeb wettable powder volume increase 7.84% more precious than Jiangsu occupies first；The biological agriculture of embodiment 2
100g/ mus of spraying yield of medicine preparation are 3104.6kg/ mus, than clear water control volume increase 13.86%, big gloomy -58% first more precious than Jiangsu
White mancozeb wettable powder volume increase 5.28%, occupies second；50% MnZn flumorph wettable powder volume increase 13.69%, 56%
Third 80g/ mus of gloomy cymoxanil wettable powder volume increase 10.43%；The volume increase of 18.7% 100g/ mus of ester water dispersible granules of alkene acyl pyrazoles
9.12%, remaining medicament yield is below precious big gloomy -58% first frost mancozeb wettable powder in Jiangsu.
Each processing group yield result table of table 4
Comprehensive analysis, -58% 100g/ mus of first frost mancozeb wettable powder of Sichuan state light spraying preventive effect and yield occupy first
Position, 2 biological pesticide preparation of embodiment 100g/ mus of spraying preventive effects and yield occupy second, 50% MnZn flumorph wettable
100g/ mus of pulvis spraying preventive effects and yield occupy third position, spraying anti-of the 60% the third 80g/ mus of gloomy cymoxanil wettable powders
Effect and yield occupy the 4th, and 18.7% alkene acyl pyrazoles 100g/ mus of ester water dispersible granules spraying preventive effects and yield occupy the 5th
Position, should widely popularize application；Precious big gloomy -58% first frost mancozeb wettable powder in Jiangsu, Fluoxastrobin suspending agent, mandipropamid are outstanding
Floating agent can be used as collocation pesticide alternating spray and use.
In conclusion the pesticidal preparations that amino-oligosaccharide and carvacrol rational mixture are prepared are used to prevent and treat by the present invention
The late blight of potato, control efficiency is significant, and can be improved yield, environmentally friendly noresidue, is Modern Green ecological agriculture development
The ideal substitute of middle chemical pesticide.
The above various embodiments has done more description in detail to the present invention, but on the basis of the present invention, can make one to it
Modify or improve, this will be apparent to those skilled in the art, therefore, without departing from the present invention claims on the basis of
Modifications or improvements belong to the scope of protection of the invention.
Claims (8)
1. a kind of for preventing and treating the biological pesticide preparation of the late blight of potato, which is characterized in that including following weight percent
Raw material components：Amino-oligosaccharide 1-5%, carvacrol 2-10%, solvent complement to 100%.
2. according to claim 1 a kind of for preventing and treating the biological pesticide preparation of the late blight of potato, which is characterized in that institute
Stating solvent includes edible alcohol and distilled water.
3. according to claim 2 a kind of for preventing and treating the biological pesticide preparation of the late blight of potato, which is characterized in that institute
Stating solvent further includes acetum.
4. according to claim 1 a kind of for preventing and treating the biological pesticide preparation of the late blight of potato, which is characterized in that institute
State the sophorolipid that raw material components further include 0.5-2%.
5. according to claim 2 a kind of for preventing and treating the biological pesticide preparation of the late blight of potato, which is characterized in that institute
The weight percent for stating edible alcohol is 8-12%, and distilled water complements to 100%.
6. according to claim 3 a kind of for preventing and treating the biological pesticide preparation of the late blight of potato, which is characterized in that institute
The concentration expressed in percentage by volume for stating acetum is 0.5-2%, and weight percent of the acetum in raw material components is 5-10%.
7. for preventing and treating the preparation method of the biological pesticide preparation of the late blight of potato described in a kind of claim 1, feature exists
In detailed process is as follows：
It requires to weigh carvacrol and amino-oligosaccharide by weight ratio, carvacrol is dissolved with edible alcohol, be A liquid；By amino
Oligosaccharide is dissolved with acetum, is B liquid；A liquid and B liquid and sophorolipid are added in distilled water, high speed shear 15-25 minutes,
It is completely dispersed mixture uniformly to get product biological pesticide preparation.
8. it is any described for preventing and treating the application method of the biological pesticide preparation of the late blight of potato in a kind of claim 1-6,
It is characterized in that, carrying out potato leaf surface sprinkling after preparation is diluted with water 400-1000 times, or used in potato planting
It states after dilution impregnates seed and plants.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112314282A (en) * | 2020-10-13 | 2021-02-05 | 中国农业科学院植物保护研究所 | Green prevention and control method for potato late blight |
| CN116058368A (en) * | 2023-03-01 | 2023-05-05 | 华植河北生物科技有限公司 | Pesticide composition containing carvacrol, preparation and application thereof |
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