CN112314282A - Green prevention and control method for potato late blight - Google Patents

Green prevention and control method for potato late blight Download PDF

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Publication number
CN112314282A
CN112314282A CN202011093060.4A CN202011093060A CN112314282A CN 112314282 A CN112314282 A CN 112314282A CN 202011093060 A CN202011093060 A CN 202011093060A CN 112314282 A CN112314282 A CN 112314282A
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dosage
prevention
potato
control method
green
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杨阳
郑永权
解钧
刘新刚
吴小虎
董丰收
徐军
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Institute of Plant Protection of Chinese Academy of Agricultural Sciences
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Institute of Plant Protection of Chinese Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08Oxygen or sulfur directly attached to an aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46N-acyl derivatives
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/72Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
    • A01N43/80Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/12Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/12Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
    • A01N47/14Di-thio analogues thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/24Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing the groups, or; Thio analogues thereof

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
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  • Agronomy & Crop Science (AREA)
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Abstract

A method for preventing and treating potato late blight in a green, safe and effective mode achieves the purposes of preventing and treating diseases and reducing the residual quantity of pesticides in potatoes and soil by selecting the potato variety and a bactericide in a matching manner and optimizing a spraying mode, and remarkably improves the effect of preventing and treating the late blight while controlling and reducing pesticide residues.

Description

Green prevention and control method for potato late blight
Technical Field
The invention relates to the technical field of prevention and control of late blight of crops, in particular to a green prevention and control technology of potato late blight.
Background
The inland abdominal land in the place of Dixi city has complex topography, large climate difference, drought and rain in the middle and north parts, high cold and yin-wet in the south part, the climate type of the inland abdominal land belongs to a south temperate zone semi-humid-middle temperate zone semi-arid region, and the continental monsoon climate is obvious. The solar energy and heat collecting device has the general characteristics of abundant light energy, insufficient heat resources, rare rainfall, large variability, dry climate and frequent meteorological disasters in the same season of rain and heat. The annual average temperature is 5.7-7.7 ℃, the annual precipitation is 400-600 mm, the frost-free period is 142 days, the sunshine is sufficient, and the potato planting method is suitable for planting potatoes. Dixi potato, a specialty of Dixi City of Gansu province, a geographical marker product of China. Over twelve years, the potato planting scale in Dengxi tends to be stable, the industrial benefit is increased continuously, the breeding system is improved continuously, the good variety promotion pace is accelerated, the industrial chain is extended effectively, and the technology integration is mature day by day. Through a continuous effort, the Mingxi city of Gansu province becomes one of three main production areas that the productivity of the virus-free seed potatoes is the first in the whole country and the potato planting is the first in the whole country, and the produced potatoes are good in quality. When 2016 is reached, 302.14 ten thousand mu of potato planting is completed in the city of Ding xi, wherein 120 ten thousand mu of special staple food variety is planted, and 271.04 ten thousand mu of standardized demonstration base is established; 93.6 thousands seats of various storage cellars (storehouses) are built, and the storage capacity of potatoes in the city of Dingxi steadily breaks through 350 thousands tons. With the increasing demand for the quality of potatoes in the market, the demand for green organic ecological potatoes is increasing day by day, and the pest and disease damage and pesticide residue pollution undoubtedly become obstacles for hindering the improvement of the quality of potatoes. The potatoes have various plant diseases and insect pests during planting and storage, and the current main prevention measures are chemical prevention and control. With the improvement of the potato on the food domination schedule in China, the planting scale of the potato is greatly increased, more pesticide preparations can be applied to the potato due to the purposes of saving labor, facilitating management, improving yield and the like in the planting process, and on the other hand, some pesticide products can be used due to the need of keeping a large number of products fresh in the storage process, so that the pesticide residue in the potato is greatly increased compared with the current pesticide residue.
The main diseases of the potato comprise potato late blight, potato virus diseases (leaf roll virus disease, mosaic virus disease, purple top and the like), potato ring rot, potato early blight, potato black shank, rhizoctonia disease and the like. The potato late blight occurs in different regions and varieties in the city of Dixi province to different degrees, and the average disease field rate is 68.2%. The occurrence area accounts for 45.35 percent of the sowing area of the potatoes, the average incidence rate is 78.4 percent, and the average yield loss rate is 17.15 percent. Therefore, a safe and effective method for preventing and treating potato late blight is urgently found, and green prevention and treatment means such as agricultural prevention and treatment, physical prevention and treatment, chemical prevention and treatment and the like are developed for preventing and treating the late blight.
Disclosure of Invention
The invention provides the following technical scheme:
a method for preventing and controlling potato late blight comprises the following steps,
(1) selecting potato variety as the virus-free seed potato No. 9;
(2) in the period from tuber growing period to mature period, the bactericide is applied for 6 times at an interval of 8-15 days, and the following bactericides are used alternately for each time:
firstly, the dosages of Baodanseng and the essence phenol are respectively 180-220 g/mu and 60-90 mL/mu;
amixida, dosage: 70-80 mL/mu;
③ arsenicum sablimatum, the dosage: 190-;
fourthly, the dosage of the syrup is: 180-220 g/mu;
green, dosage: 10-30 mL/mu;
sixthly, the consumption of the Yinfari, the jarosite and the pyraclostrobin is respectively 90-120 mL/mu, 50-80 g/mu and 60-90 mL/mu (the concentration is 25%).
According to the prevention and control method of the present invention, in the stage from the stem tuber growing period to the maturing period in step (2), the bactericide is applied once more after 6 times, and the following bactericides are used: seventhly, increasing the power and winning green, and the dosage: 10-30 mL/mu.
According to the prevention and control method, the unmanned aerial vehicle is used for implementing the medicine application in the step (2).
According to the prevention and control method, between the seedling stage before the step (2) and the tuber growing stage, the method also comprises the step (1-2) of mechanically or artificially weeding or further weeding by using a herbicide. Preferably, the herbicide is used 1 time. Preferably, the herbicide is applied to the field, and the dosage is 150-200 mL/mu.
According to the prevention and control method, after the step (1-2), the step (1-3) of using the pesticide is further included. Preferably, the mixture is applied by mixing 10% phoxim granules with fine soil during sowing.
According to the prevention and control method, before seeding, the steps of weeding, deep ploughing and fertilizing are also included. Preferably, the fertilization adopts 80 kg/mu of nitrogen, phosphorus and potassium compound fertilizer; 1.5 t/mu of organic fertilizer; and topdressing for three times. Preferably, the topdressing is carried out three times, wherein calcium ammonium nitrate (N/Ca/Mg-7/24/6) is sequentially applied at intervals of 20-25 days, and the dosage is 10 kg/mu; water-soluble fertilizer (N/P/K is 10/5/5), 10 kg/mu; and water-soluble fertilizer (N/P/K is 10/5/5), 10 kg/mu and zinc sulfate 200 g/mu.
According to the prevention and control method, irrigation is carried out for 4-5 times in the whole growth period. Preferably, the total irrigation amount is generally 1500-1875 m3/hm2. Preferably, the water is irrigated for 1 time after seedling emergence, 2 times in the flowering phase, 1-2 times in the tuber expansion phase, and the irrigation quantity is 375m each time3/hm2
The invention also provides a bactericide composition for preventing and treating potato late blight, which comprises the following components:
firstly, the dosages of Baodanseng and the essence phenol are respectively 180-220 g/mu and 60-90 mL/mu;
amixida, dosage: 70-80 mL/mu;
③ arsenicum sablimatum, the dosage: 190-;
fourthly, the dosage of the syrup is: 180-220 g/mu;
green, dosage: 10-30 mL/mu;
sixthly, the consumption of the Yinfari, the jarosite and the pyraclostrobin is respectively 90-120 mL/mu, 50-80 g/mu and 60-90 mL/mu (the concentration is 25%).
According to the composition of the present invention, the above components are applied alternately in sequence.
The composition according to the present invention, wherein component # Zengwei Yilv is applied again after component # The.
Has the advantages that: the invention provides a green, safe and effective method for preventing and treating potato late blight, integrates green prevention and treatment means such as agricultural prevention and treatment, physical prevention and treatment, chemical prevention and treatment and the like, reduces the dosage of a medicament, thereby achieving the purposes of preventing and treating diseases and reducing the residual quantity of pesticides in potatoes and soil, and the invention realizes the effects of remarkably improving the late blight epidemic prevention while controlling and reducing pesticide residues.
Detailed Description
The compounds of the present invention, methods for their preparation and their use are described in further detail in the following examples. It is to be understood that the following examples are only illustrative and explanatory of the present invention and should not be construed as limiting the scope of the present invention. All the technologies realized based on the above-mentioned contents of the present invention are covered in the protection scope of the present invention. Unless otherwise indicated, the raw materials and reagents used in the following examples are all commercially available products or can be prepared by known methods.
Microbial inoculum and chemical agent (the percentage is mass percentage content) referred to in the following examples.
The potato variety used in the following examples was the virus-free seed potato number 9.
And (3) field popularization: cangzhou Runde pesticide Co Ltd
Imidacloprid: generous agricultural chemical
Phoxim: shandong Shengbanglunan pesticide Co Ltd
Acetamiprid: guangxi Bye Biotech Co Ltd
Baodasen: jiangsu Baoling
Amixida: zuis Zhan Xianzhengda
And (3) killing alum: zuis Zhan Xianzhengda
And (3) g, syrup: dupont
Green in increasing power: dupont
Silver method: bayer process
25% pyraclostrobin suspension concentrate: pasf Germany
Example 1 evaluation of the prevention and control Effect of the Green prevention and control method of the present invention on the late blight of Potato
The plant used in this example was potato, variety # 9. 2 treatments were set, respectively for the green prevention and control group (demonstration area of the invention) and the control group (farmer base)
Green prevention and control group:
first stage (before sowing-seedling stage)
1) Before the planting activities are carried out, the seed selection link is strictly controlled, and the planted variety is selected according to local conditions by combining the actual conditions of the planting area. In the potato seed selection process, seeds need to be screened, and seeds which are disease-free and have high health are selected, so that the seeds are prevented from carrying germs. And selecting the virus-free seed potato number 9.
2) Weeding management is carried out on the field, and the pest growth environment is damaged to the greatest extent.
3) Deep ploughing the soil to improve the disease resistance. Provides effective fertilizer for potatoes, increases soil organic elements, such as potassium fertilizer and phosphate fertilizer content in soil, and comprehensively improves the disease resistance of plants. Compound fertilizer (N/P/K10/15/20, six countries chemical industry), 80 kg/mu; 1.5 t/mu of organic fertilizer; topdressing: three times, 6 months and 20 days: 10 kg/mu of calcium ammonium nitrate (N/Ca/Mg: 7/24/6); 7, month and 15 days: water-soluble fertilizer (N/P/K10/5/5, Fuqiang brand), 10 kg/mu; ③ 8 months and 5 days: water-soluble fertilizer (N/P/K10/5/5, Fuqiang brand), 10 kg/mu; zinc sulfate is 200 g/mu (drip irrigation).
4) And (3) reasonably performing rotation: the potato is a solanaceae plant, can not be in rotation with tobacco, pepper, eggplant and other plants, can not cause serious diseases, and can be in rotation with bean crops and fibrous plants.
5) Adopting a drip irrigation mode, irrigating for 4-5 times in the whole growth period, wherein the irrigation quota is generally 1500-1875 m3/hm2Left and right.
Irrigating for 1 time after seedling emergenceIrrigating for 2 times in the period, irrigating for 1-2 times in the tuber expansion period, and irrigating water quantity of 375m each time3/hm2
Second stage (seedling stage-tuber growth stage)
1) Mechanical or artificial weeding is adopted. Reducing the weeding of chemical drugs. If necessary, herbicides can be used for weeding.
Herbicide: 1 time, sealing the soil before seeding after sowing
And (3) field popularization: the recommended dosage of 330g/L pendimethalin, EC and potato is 150-
2) Chemical insect prevention: when sowing, 2.5 kg/mu of 10% phoxim granules are mixed with 20kg of fine soil for spreading.
Third stage (tuber growth-mature)
The stage is a key period for improving the yield and quality of the potato tubers, the plants are dense and closed, the physiological disease resistance of the potatoes reaches the lowest point, the growing environment becomes more humid along with the coming of rainy seasons, and the late blight is very easy to occur at the moment. The unmanned aerial vehicle is popularized to be applied to the aspect of potato disease and pest control, and multiple bactericides are used alternately, so that the drug resistance is reduced, and the use of chemical agents is reduced. The bactericide is applied 6-7 times (6 times in this example), and the following bactericide numbered (r) -c or the bactericide numbered (r) -c is used alternatively, and the interval between each application of the bactericide is 12 days. The first application is completed in 6 days due to the large planting area, and the first application date of the invention is 6 months and 15 days to 6 months and 20 days.
Actual dosage of DaSen Bao: 200 g/mu, and the actual dosage of the essence phenol: 80 mL/mu
Baodasen: metalaxyl 10%, mancozeb 48%;
WP (wettable powder);
② practical dosage of amisida: 70-80 mL/mu
Amixida: 250g/L azoxystrobin;
SC (suspending agent);
③ actual dosage of alum: 200 g/mu
And (3) killing alum: 11.2 percent of oxadixyl, 17.8 percent of mancozeb;
WP (wettable powder).
Fourthly, actual dosage of the syrup: 200 g/mu
And (3) g, syrup: 8% cymoxanil, 64% mancozeb;
WP (wettable powder);
increasing green actual dosage: 20 mL/mu
Green in increasing power: 10% oxathiapiprolin;
OD (oil suspension);
sixthly, the actual dosage of the silver method: 110 mL/mu, actual dosage of alum: 60-70 g/mu, and 25% pyraclostrobin suspending agent 80 ml/mu, 2500 times liquid
Silver method: 625g/L propamocarb hydrochloride, 62.5g/L fluopicolide;
SC (suspending agent);
and (3) killing alum: 11.2 percent of oxadixyl, 17.8 percent of mancozeb;
WP (wettable powder).
Seventhly, increasing the actual dosage of the Yingjiu green: 20 mL/mu
Green in increasing power: 10% oxathiapiprolin;
OD (oil suspension).
Control group:
weeds: after the membrane surface is covered with soil in the middle and last ten days of 5 months, 80-100ml of 2-methyl-pentaphospho sealant is added into each acre, 15kg of water is added into each acre for spraying, and the germ tissues of the weeds are damaged.
Aphids: 20 percent of virus A500-fold liquid, 1.5 percent of carbendazim 1000-fold liquid, 10 percent of imidacloprid 1500-fold liquid, 3 percent of acetamiprid 3000-fold liquid and other pesticides are selected to be alternately sprayed in combination with late blight prevention for preventing and controlling aphids.
Late blight: from the beginning of seedling stage (complete seedling emergence), the following medicaments are selected and applied in turn every 7-10 days, wherein the 25% metalaxyl wettable powder is 150 g/mu, and is diluted by 800-1000 times of liquid; 150 g/mu of 58% metalaxyl manganese zinc wettable powder and 400-500 times of liquid; 150 g/mu of 64% chalcanthite wettable powder and 400-500 times of solution; 150 g/mu of 72% gravy wettable powder and 600-800 times of liquid; 150 g/mu of 80% wettable zineb powder and 500-600 times of liquid; the Yinfari is 110 mL/mu; 80 mL/mu of 25% pyraclostrobin and 1500 times of solution; the pesticide is sprayed alternately in sequence to prevent and treat early and late blight as soon as possible. Spraying for 5-6 times in the whole growth period.
The result evaluation method comprises the following steps:
method for detecting final residual quantity in potatoes and soil
In the potato demonstration area of Gansu Dengxi, potatoes and soil at different sites of a control group and a green prevention and control group are sampled, and a 5-point method random sampling method is adopted for sampling according to pesticide registration residue test criteria (NY/T788-2004) and pesticide registration residue field test standard operating procedures (pesticide institute of Ministry of agriculture, 2007) to collect samples.
Potato tubers (at least 4-5, not less than 2 kg): the adhered soil and residue were gently brushed away with a brush, and the sample was cut into 1cm cubes with a stainless steel knife and thoroughly mixed in a stainless steel basin. Dividing by quartering method, dividing into 150g two parts, placing into sample container, and labeling (-20 deg.C) for storage.
Soil: and 5 sampling points are randomly selected for sampling, and each point presses the soil sampler into the depth of 10cm in a reciprocating rotation mode. And pulling out the soil drill to enable the opening of the side groove of the soil drill to be upward, removing the soil sample below the tip scale by using a screwdriver, and prying the soil core part in the sampler into the stainless steel basin. Collecting 1-2 kg of soil samples, and removing impurities such as broken stones, weeds, plant roots and stems. After the samples collected in the field are mixed evenly, two samples of 200g are reserved by a quartering method, and the samples are put into a sample container to be frozen at (-20 ℃) for storage.
The amount of pesticide residue in the sample was calculated according to the following formula:
Figure BDA0002722809470000071
wherein R is the residual quantity (mg/kg) of the pesticide to be detected in the sample; c is expressed as standard solution concentration (mg/L); v is labeled standard solution injection volume (μ L); v is finally the final constant volume (mL) of the sample solution; sample V is sample injection volume (μ L) of the sample solution; the S sample is the peak area of the sample solution; s is marked as the peak area of the standard solution; w is the mass (g) of the sample; a is the concentration multiple of the target in the method.
Test results
Potato tuber
In the potato demonstration area of Gansu Dingxi, the late blight of potato can be effectively prevented and controlled. We have proposed pesticide residue detection in a green integrated control scheme. Through detection, the index residual amounts of azoxystrobin, pyraclostrobin and imidacloprid in the potato tubers of the control group are respectively 0.067, 0.218 and 0.0093mg/kg, the corresponding indexes of the demonstration area are respectively 0.044mg/kg, and the indexes are not detected and detected, the residual rates are respectively reduced by 34%, 100% and 100%, and the expected requirements of 20% -25% are met (see table 1). The residual amount of oxadixyl in potato tubers in the demonstration area was 0.00321, which is below the maximum residual limit specified by the national standard despite being higher than the control group, with no dietary risk. The fitting degree (R2 is more than or equal to 0.9986) of the standard curve for detecting the pesticide in the potato tubers and the addition recovery rate (67.7-130.2%) meet the requirements.
TABLE 1 pesticide residue in potato tubers of Gansu Dingxi City and control group
Figure BDA0002722809470000072
Figure BDA0002722809470000081
Indicates significant difference compared to control group (p <0.05)
Potato soil
In the potato demonstration area of Gansu Dingxi, the late blight of potato can be effectively prevented and controlled. We performed residue detection on the pesticides suggested in the green integrated control scheme. In addition, pesticide residue detection is carried out on other potatoes which are conventionally used. Wherein the demonstration zone fluopicolide is higher than the control group, but lower than the maximum residual limit specified in the national standard, and has good control effect, is not detected in potato tubers, and has no diet risk (see table 2). The fitting degree (R2 is more than or equal to 0.9981) of the standard curve of the pesticide in the potato soil and the addition recovery rate (71.4-111.7%) meet the requirements.
TABLE 2 pesticide residue in soil of potatoes of Gansu Dingxi City green prevention and control group
Figure BDA0002722809470000082
Figure BDA0002722809470000091
Potato late blight in demonstration and control areas
In late 8 th month, evaluating the late blight incidence of the demonstration area and the control area (yellow brown leaves, brown strip spots on stems, and potatoes with brown or purple disease spots on potato blocks are regarded as late blight), and counting according to the disease area measurement: the area of the demonstration zone occupied by the late blight was about 5%, while the control zone was about 30% -35%.
According to the test results of the embodiment of the invention, the green prevention and control integration scheme of the potato late blight has the advantages that the pesticide residue is greatly reduced in the aspect of controlling the pesticide residue, especially the pesticide residue in the potato stem blocks, the diet risk is reduced, and the green prevention and control integration scheme of the invention has a remarkable improvement on disease epidemic prevention.
The embodiments of the present invention have been described above. However, the present invention is not limited to the above embodiment. Any modification, equivalent replacement, improvement and the like, which are within the spirit and principle of the present invention, should be included in the protection scope of the present invention.

Claims (10)

1. A method for preventing and controlling potato late blight comprises the following steps,
(1) selecting potato variety as the virus-free seed potato No. 9;
(2) in the period from tuber growing period to mature period, the bactericide is applied for 6 times at an interval of 8-15 days, and the following bactericides are used alternately for each time:
firstly, the dosages of Baodanseng and the essence phenol are respectively 180-220 g/mu and 60-90 mL/mu;
amixida, dosage: 70-80 mL/mu;
③ arsenicum sablimatum, the dosage: 190-;
fourthly, the dosage of the syrup is: 180-220 g/mu;
green, dosage: 10-30 mL/mu;
sixthly, the dosages of the Alum chloratum and the pyraclostrobin are respectively 90-120 mL/mu, 50-80 g/mu and 60-90 mL/mu (the concentration is 25%).
2. The prevention and control method according to claim 1, wherein the bactericide is administered once more after 6 times in the stage of the tuber growth to the maturity stage in step (2), and the following bactericides are used: seventhly, increasing the power and winning green, and the dosage: 10-30 mL/mu.
3. The prevention and control method according to claim 1, wherein the administering of step (2) is performed using an unmanned aerial vehicle.
4. The prevention and control method according to claim 1, further comprising the step of (1-2) applying mechanical or artificial weeding, or further weeding with a herbicide, between the seedling stage and the tuber-growing stage before the step (2). Preferably, the herbicide is used 1 time. Preferably, the herbicide is applied to the field application in an amount of 150-200 mL/mu.
5. The prevention and control method according to claim 1, further comprising the step of (1-3) applying an insecticide after the step of (1-2). Preferably, the mixture is applied by mixing 10% phoxim granules with fine soil during sowing.
6. The prevention and control method according to claim 1, further comprising the steps of weeding, deep ploughing, and fertilizing before sowing. Preferably, the fertilization adopts 80 kg/mu of nitrogen, phosphorus and potassium compound fertilizer; 1.5 t/mu of organic fertilizer; and topdressing for three times. Preferably, the topdressing is carried out three times, wherein calcium ammonium nitrate (N/Ca/Mg-7/24/6) is sequentially applied at intervals of 20-25 days, and the dosage is 10 kg/mu; water-soluble fertilizer (N/P/K is 10/5/5), 10 kg/mu; and water-soluble fertilizer (N/P/K is 10/5/5), 10 kg/mu and zinc sulfate 200 g/mu.
7. The prevention and control method according to claim 1,irrigating for 4-5 times in the whole growth period. Preferably, the total irrigation amount is generally 1500-1875 m3/hm2. Preferably, the water is irrigated for 1 time after seedling emergence, 2 times in the flowering phase, 1-2 times in the tuber expansion phase, and the irrigation quantity is 375m each time3/hm2
8. A bactericide composition for preventing and treating potato late blight contains the following components:
firstly, the dosages of Baodanseng and the essence phenol are respectively 180-220 g/mu and 60-90 mL/mu;
amixida, dosage: 70-80 mL/mu;
③ arsenicum sablimatum, the dosage: 190-;
fourthly, the dosage of the syrup is: 180-220 g/mu;
green, dosage: 10-30 mL/mu;
sixthly, the consumption of the Yinfari, the jarosite and the pyraclostrobin is respectively 90-120 mL/mu, 50-80 g/mu and 60-90 mL/mu (the concentration is 25%).
9. The composition according to claim 8, wherein said components are applied in succession.
10. A composition according to claim 9 wherein component # zengweilv is applied again after component # xiv.
CN202011093060.4A 2020-10-13 2020-10-13 Green prevention and control method for potato late blight Pending CN112314282A (en)

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