CN113273467A - Microbial inoculum combination for pseudo-ginseng planting and use method thereof - Google Patents
Microbial inoculum combination for pseudo-ginseng planting and use method thereof Download PDFInfo
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- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 132
- 244000131316 Panax pseudoginseng Species 0.000 title claims abstract description 81
- 235000003181 Panax pseudoginseng Nutrition 0.000 title claims abstract description 80
- 238000000034 method Methods 0.000 title claims description 22
- 241000180649 Panax notoginseng Species 0.000 claims abstract description 37
- 235000003143 Panax notoginseng Nutrition 0.000 claims abstract description 37
- 241000223260 Trichoderma harzianum Species 0.000 claims abstract description 24
- 229920001661 Chitosan Polymers 0.000 claims abstract description 20
- 239000000126 substance Substances 0.000 claims abstract description 17
- 239000002023 wood Substances 0.000 claims abstract description 17
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 14
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 14
- 241000589540 Pseudomonas fluorescens Species 0.000 claims abstract description 14
- 239000003337 fertilizer Substances 0.000 claims abstract description 12
- 241000233866 Fungi Species 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 74
- 239000003085 diluting agent Substances 0.000 claims description 56
- 241000894006 Bacteria Species 0.000 claims description 37
- 238000007865 diluting Methods 0.000 claims description 32
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- 238000002156 mixing Methods 0.000 claims description 32
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- 239000003814 drug Substances 0.000 claims description 21
- 239000002689 soil Substances 0.000 claims description 20
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- 238000005507 spraying Methods 0.000 claims description 18
- 238000002360 preparation method Methods 0.000 claims description 16
- 239000012895 dilution Substances 0.000 claims description 14
- 238000010790 dilution Methods 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 14
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- 238000002054 transplantation Methods 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 5
- 230000007480 spreading Effects 0.000 claims description 4
- 238000003892 spreading Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 abstract description 3
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- 239000000447 pesticide residue Substances 0.000 abstract description 2
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- 244000052616 bacterial pathogen Species 0.000 description 5
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- 241000208340 Araliaceae Species 0.000 description 1
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- 229920002678 cellulose Polymers 0.000 description 1
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- 150000001875 compounds Chemical class 0.000 description 1
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- 238000009776 industrial production Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
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- 229910052698 phosphorus Inorganic materials 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Abstract
The microbial inoculum composition for planting pseudo-ginseng comprises one or more of wood rot fungi, bacillus subtilis, pseudomonas fluorescens, trichoderma harzianum and chitosan. The application discloses a series of microbial inoculum combinations for pseudo-ginseng planting aiming at the environment and self factors of pseudo-ginseng planting, and the microbial inoculum combinations comprise four microbial inoculum A, four microbial inoculum B, four microbial inoculum C and four microbial inoculum D according to different planting environments and product quality requirements, so that different microbial inoculum environment requirements of pseudo-ginseng planting on old land multiple planting backgrounds, organic pseudo-ginseng planting backgrounds and green pseudo-ginseng planting backgrounds are met. Under the background of old land multiple planting management, the root rot of the pseudo-ginseng can be effectively inhibited, and the survival rate of the pseudo-ginseng is improved; under the background of organic panax notoginseng planting, the yield of panax notoginseng is obviously improved; under the background of green panax notoginseng planting, the use amount of chemical fertilizer is greatly reduced, the risk of pesticide residue at the root of panax notoginseng is reduced, and the quality of panax notoginseng is improved.
Description
Technical Field
The invention relates to the technical field of pseudo-ginseng planting, in particular to a microbial inoculum combination for pseudo-ginseng planting and a using method thereof.
Background
Notoginseng, the name of traditional Chinese medicine. Is dried root of Panax notoginseng (Burk.) F.H.Chen of Araliaceae. Has effects of removing blood stasis, stopping bleeding, promoting blood circulation, and relieving pain. It can be used for treating hemorrhage, traumatic injury, and swelling and pain due to blood stasis.
Pseudo-ginseng is mainly produced in counties of wenshan, yunnan, inkstone, guan, west, guan, mahuang, mahonia, funing, qibei, etc., and is also planted in Guangxi Tianyang, Jingxi, Tiandong, Debao, etc. Yunnan Wenshanzhou has long history, large yield and good quality, and is commonly called as Wen pseudo-ginseng and Tian Qi, which are famous genuine medicinal materials.
The microbial agent is a viable bacteria preparation prepared by processing fermentation liquor for adsorbing bacteria by using porous substances as adsorbents (such as turf and vermiculite) after industrial production and propagation of target microorganisms (effective bacteria). The bactericide is used for dressing seeds or dipping roots, and has the effects of directly or indirectly improving soil, restoring land capability, preventing soil-borne diseases, maintaining rhizosphere microflora balance, degrading toxic and harmful substances and the like. The agricultural microbial agent can be properly used for improving the yield of agricultural products, improving the quality of the agricultural products, reducing the using amount of chemical fertilizers, reducing the cost, improving the soil and protecting the ecological environment.
In order to improve the yield and the quality of pseudo-ginseng and ensure the soil quality, a microbial inoculum needs to be applied to a pseudo-ginseng field, the growth requirement of pseudo-ginseng cannot be met by a single microbial inoculum, and the existing microbial inoculum combination is not suitable for planting of pseudo-ginseng, so that a microbial inoculum needs to be prepared to meet the growth requirement of pseudo-ginseng. The current pseudo-ginseng planting has the following outstanding problems.
Firstly, pseudo-ginseng has serious continuous sitting obstacle, and continuous cultivation on the same land can cause partial non-seedling, non-vigorous seedling or dead seedling of pseudo-ginseng. At present, although the soil fumigation technology can be used for treating the soil to reduce the damage of the panax notoginseng continuous sitting obstacle and realize the old land multiple cropping, the probability of seedling death caused by panax notoginseng root rot in the old land multiple cropping management is very high, so that one of key control points in the old land multiple cropping management is generated by how to prevent the panax notoginseng root rot in the old land multiple cropping management.
Secondly, the production is carried out according to organic planting management regulations, the requirement of organic production on input products is strict, so that the yield of the organic pseudo-ginseng is low, and the problem of how to solve the yield of the organic pseudo-ginseng without violating the organic planting management regulations is a big pain point of organic pseudo-ginseng planting management.
Thirdly, cultivation is carried out according to pollution-free planting management standards, and the investment of fertilizers and chemical pesticides is advocated to be reduced in the green panax notoginseng planting and cultivation process, so that the residues of harmful substances in panax notoginseng products are reduced. Reducing the input of fertilizer pesticides can cause a substantial reduction in yield.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a microbial inoculum combination for pseudo-ginseng planting and a using method thereof.
In order to achieve the purpose, the invention adopts the technical scheme that:
the microbial inoculum composition for planting pseudo-ginseng comprises one or more of wood rot fungi, bacillus subtilis, pseudomonas fluorescens, trichoderma harzianum and chitosan.
Further, the microbial inoculum combination comprises a microbial inoculum A, a microbial inoculum B, a microbial inoculum C and a microbial inoculum D;
and (3) microbial inoculum A: comprises bacillus subtilis, trichoderma harzianum, chitosan and water;
and (3) bacterial agent B: consists of Trichoderma harzianum, Pseudomonas fluorescens and water;
and (3) microbial inoculum C: consists of wood decay bacteria liquid, sugar and water;
and (3) microbial inoculum D: consists of wood decay bacteria liquid and water.
Further, the preparation method of the microbial inoculum A comprises the following steps: weighing Trichoderma harzianum and Trichoderma harzianum according to the proportion of 1:1 in the amount of 600-1300 g/mu respectively per mu, and diluting with 5-10L of clear water to obtain a diluent a; measuring 1-2L of chitosan, and diluting with 5-10L of clear water to obtain a diluent b; mixing the diluent a and the diluent b, adding clear water for secondary dilution to 400-500L, and uniformly mixing for later use;
the preparation method of the microbial inoculum B comprises the following steps: weighing bacillus subtilis and pseudomonas fluorescens at a ratio of 1:1 in an amount of 600-1300 g/mu respectively per mu, and diluting with 5-10L of clear water to obtain a diluent c; measuring 1-2L of chitosan, and diluting with 5-10L of clear water to obtain a diluent d; mixing the diluent c and the diluent d, adding clear water for secondary dilution to 400-500L, and uniformly mixing for later use;
the preparation method of the microbial inoculum C comprises the following steps: measuring wood decay bacteria liquid according to the use amount of 3-4L/mu per mu, and diluting with clear water to obtain a diluent e; taking 8-12 kg of sugar, and dissolving with 10-20L of clear water to obtain a diluent f; mixing the diluent e and the diluent f, adding clear water for secondary dilution to 400-500L, and uniformly mixing for later use;
the preparation method of the microbial inoculum D comprises the following steps: measuring the wood decay bacteria liquid according to the use amount of 1-2L/mu per mu, diluting the wood decay bacteria liquid with clear water, diluting the wood decay bacteria liquid to 400-500L for the second time, and uniformly mixing the wood decay bacteria liquid for later use.
The use method of the microbial inoculum combination for planting the pseudo-ginseng comprises the following steps:
under the background of old land multiple cropping management, after the transplantation of pseudo-ginseng seedlings is completed, selecting a microbial inoculum A, preparing a bacterial solution, connecting a medicine inlet and a watering opening, adjusting the size of the medicine inlet, opening a water pump, and spraying pseudo-ginseng; the prepared microbial inoculum A is applied once every 30-60 days, 4-6 times in the whole year and is continuously used for 2 years;
under the background of organic panax notoginseng planting management, after the panax notoginseng seedlings are transplanted, selecting a microbial inoculum B, preparing a bacterial liquid, connecting a medicine inlet and a watering opening, adjusting the size of the medicine inlet, opening a water pump, and spraying panax notoginseng; the prepared microbial inoculum B is applied once every 30-60 days, 4-6 times in the whole year and is continuously used for 2 years;
under the background of green pseudo-ginseng planting management, after pseudo-ginseng seedlings are transplanted, uniformly spreading a layer of wood particles on the soil surface, and covering pine needles on the upper layer of the wood particles; after covering the pine needles, starting to apply the bacteria solution, selecting the microbial inoculum C for the first time, and spraying pseudo-ginseng by using an application machine; applying a second microbial inoculum after 7-10 days of interval, selecting microbial inoculum D for the second time, preparing a bacterial solution, and spraying pseudo-ginseng by using an application machine; after the second application, the prepared microbial inoculum D is applied once every 10-20 days, is used for 20-24 times every year and is continuously used for 2 years; chemical fertilizer is not added in the planting process, and the chemical pesticide input is reduced by half.
Further, when the microbial inoculum is used, the temperature in the greenhouse is 18-25 ℃.
Furthermore, when the microbial inoculum is used, the soil humidity is controlled to be 50-70 percent and not more than 85 percent.
Further, under the background of organic panax notoginseng planting management, 400-500L of the prepared fungicide B is applied to each mu, and the irrigation quantity is 4-6 m 3.
Further, under the background of green panax notoginseng planting management, the consumption of wood particles is 1300-2600 kg/mu; the pine needle dosage is 1200-1800 kg/mu.
Further, 400-500L of the prepared fungicide C is applied to each mu; the prepared microbial inoculum D is applied by 300-400L per mu.
Compared with the prior art, the invention has at least the following beneficial effects:
the application discloses a series of microbial inoculum combinations for pseudo-ginseng planting aiming at the environment and self factors of pseudo-ginseng planting, and the microbial inoculum combinations comprise four microbial inoculum A, four microbial inoculum B, four microbial inoculum C and four microbial inoculum D according to different planting environments and product quality requirements, so that different microbial inoculum environment requirements of pseudo-ginseng planting on old land multiple planting backgrounds, organic pseudo-ginseng planting backgrounds and green pseudo-ginseng planting backgrounds are met. Under the background of old land multiple planting management, the root rot of the pseudo-ginseng can be effectively inhibited, and the survival rate of the pseudo-ginseng is improved; under the background of organic panax notoginseng planting, the yield of panax notoginseng is obviously improved; under the background of green panax notoginseng planting, the use amount of chemical fertilizer is greatly reduced, the risk of pesticide residue at the root of panax notoginseng is reduced, and the quality of panax notoginseng is improved.
Detailed Description
Example 1:
the microbial inoculum composition for planting pseudo-ginseng comprises one or more of wood rot fungi, bacillus subtilis, pseudomonas fluorescens, trichoderma harzianum and chitosan.
Example 2:
on the basis of the embodiment 1, the microbial inoculum combination comprises a microbial inoculum A, a microbial inoculum B, a microbial inoculum C and a microbial inoculum D;
and (3) microbial inoculum A: comprises bacillus subtilis, trichoderma harzianum, chitosan and water;
the chitosan can enhance the self-resistance (immunity) of plants, and the bacillus and the trichoderma are two beneficial biological bacteria which can compete with pathogenic bacteria, occupy the positions of pathogenic bacteria in soil, enable the pathogenic bacteria not to grow, achieve the aim of plant health, activate nutrient elements such as nitrogen, phosphorus, potassium and the like in the soil, improve the soil and improve the yield.
And (3) bacterial agent B: consists of Trichoderma harzianum, Pseudomonas fluorescens and water;
trichoderma is mainly used for fungus, a fluorescent rod is used for bacteria, and two colonies of the trichoderma are propagated in the soil in a large amount to occupy the positions of root rot pathogenic bacteria in the soil, so that fungus and bacterial infection in the soil can be prevented.
And (3) microbial inoculum C: consists of wood decay bacteria liquid, sugar and water;
and (3) microbial inoculum D: consists of wood decay bacteria liquid and water.
The wood-decay fungi has very strong reproductive capacity, and can be applied to the surface of a soil layer and around leaves in preference to aerobic pathogenic bacteria and algae to realize an environment which takes the wood-decay fungi as a leading factor. The fertilizer can decompose cellulose and lignin in fallen leaves and dead wood, decompose dioxin which is a compound difficult to decompose, activate soil nutrient elements, provide nitrogen sources and carbon sources for pseudo-ginseng, enhance pseudo-ginseng plant resistance, promote plant growth and achieve the effect of reducing the input of pesticides and chemical fertilizers.
Example 3:
on the basis of the embodiment 1-2, the method for preparing the microbial inoculum combination for planting the pseudo-ginseng comprises the following steps:
the preparation method of the microbial inoculum A comprises the following steps: weighing bacillus subtilis and trichoderma harzianum according to the ratio of 1:1 by using the dosage of 600-1300 g/mu respectively per mu, and diluting with 5-10L of clear water to obtain a diluent a; measuring 1-2L of chitosan, and diluting with 5-10L of clear water to obtain a diluent b; mixing the diluent a and the diluent b, adding clear water for secondary dilution to 400-500L, and uniformly mixing for later use;
the preparation method of the microbial inoculum B comprises the following steps: weighing Trichoderma harzianum and pseudomonas fluorescens at a ratio of 1:1 in an amount of 600-1300 g/mu respectively per mu, and diluting with 5-10L of clear water to obtain a diluent c; measuring 1-2L of chitosan, and diluting with 5-10L of clear water to obtain a diluent d; mixing the diluent c and the diluent d, adding clear water for secondary dilution to 400-500L, and uniformly mixing for later use;
the preparation method of the microbial inoculum C comprises the following steps: measuring wood decay bacteria liquid according to the use amount of 3-4L/mu per mu, and diluting with clear water to obtain a diluent e; taking 8-12 kg of sugar, and dissolving with 10-20L of clear water to obtain a diluent f; mixing the diluent e and the diluent f, adding clear water for secondary dilution to 400-500L, and uniformly mixing for later use;
the preparation method of the microbial inoculum D comprises the following steps: measuring the wood decay bacteria liquid according to the use amount of 1-2L/mu per mu, diluting the wood decay bacteria liquid with clear water, diluting the wood decay bacteria liquid to 400-500L for the second time, and uniformly mixing the wood decay bacteria liquid for later use.
Example 4:
on the basis of the embodiments 1-3, the use method of the microbial inoculum combination for planting the pseudo-ginseng comprises the following steps:
under the background of old land multiple cropping management, after the transplantation of pseudo-ginseng seedlings is completed, selecting a microbial inoculum A, preparing a bacterial solution, connecting a medicine inlet and a watering opening, adjusting the size of the medicine inlet, opening a water pump, and spraying pseudo-ginseng; the prepared microbial inoculum A is applied once every 30-60 days, 4-6 times in the whole year and is continuously used for 2 years;
under the background of organic panax notoginseng planting management, after the panax notoginseng seedlings are transplanted, selecting a microbial inoculum B, preparing a bacterial liquid, connecting a medicine inlet and a watering opening, adjusting the size of the medicine inlet, opening a water pump, and spraying panax notoginseng; the prepared microbial inoculum B is applied once every 30-60 days, 4-6 times in the whole year and is continuously used for 2 years;
under the background of green pseudo-ginseng planting management, after pseudo-ginseng seedlings are transplanted, uniformly spreading a layer of wood particles on the soil surface, and covering pine needles on the upper layer of the wood particles; after covering the pine needles, starting to apply the bacteria solution, selecting the microbial inoculum C for the first time, and spraying pseudo-ginseng by using an application machine; applying a second microbial inoculum after 7-10 days of interval, selecting microbial inoculum D for the second time, preparing a bacterial solution, and spraying pseudo-ginseng by using an application machine; after the second application, the prepared microbial inoculum D is applied once every 10-20 days, is used for 20-24 times every year and is continuously used for 2 years; chemical fertilizer is not added in the planting process, and the chemical pesticide input is reduced by half.
Example 5:
in addition to the examples 1 to 4, the temperature in the greenhouse was 18 ℃ to 25 ℃ when the microbial inoculum was used.
Example 6:
on the basis of the embodiments 1-5, when the microbial inoculum is used, the soil humidity is controlled to be 50% -70%, and is not more than 85%.
Example 7:
on the basis of the embodiments 1-6, under the background of organic panax notoginseng planting management, 400-500L of the prepared fungicide B is applied per mu, and the irrigation amount is 4-6 m3。
Example 8:
on the basis of the embodiments 1-7, under the background of old land multiple cropping management, 400-500L of the prepared fungicide A is applied per mu, and the irrigation amount is 4-6 m3。
Example 9:
on the basis of the embodiments 1-8, in the background of green panax notoginseng planting management, the dosage of the wood particles is 1300-2600 kg/mu; the pine needle dosage is 1200-1800 kg/mu.
Example 10:
on the basis of the embodiments 1-9, 400-500L of the prepared fungicide C is applied per mu; the prepared microbial inoculum D is applied by 300-400L per mu.
Example 11:
and (3) microbial inoculum A: comprises bacillus subtilis, trichoderma harzianum, chitosan and water;
weighing bacillus subtilis and trichoderma harzianum according to the ratio of 1:1 by using the dosage of 600-1300 g/mu respectively per mu, and diluting with 5-10L of clear water to obtain a diluent a; measuring 1-2L of chitosan, and diluting with 5-10L of clear water to obtain a diluent b; mixing the diluent a and the diluent b, adding clear water for secondary dilution to 400-500L, and uniformly mixing for later use;
after the transplantation of the pseudo-ginseng seedlings is finished, selecting the microbial inoculum A, preparing a bacterial solution, connecting a medicine inlet and a watering opening, adjusting the size of the medicine inlet,opening a water pump, and spraying pseudo-ginseng; the prepared microbial inoculum A is applied once every 30-60 days, 4-6 times in the whole year and is continuously used for 2 years; the prepared microbial inoculum A is applied by 400-500L per mu, and the irrigation quantity is 4-6 m3。
Example 12:
and (3) bacterial agent B: consists of Trichoderma harzianum, Pseudomonas fluorescens and water;
the configuration method comprises the following steps: weighing Trichoderma harzianum and pseudomonas fluorescens at a ratio of 1:1 in an amount of 600-1300 g/mu respectively per mu, and diluting with 5-10L of clear water to obtain a diluent c; measuring 1-2L of chitosan, and diluting with 5-10L of clear water to obtain a diluent d; mixing the diluent c and the diluent d, adding clear water for secondary dilution to 400-500L, and uniformly mixing for later use;
after the transplantation of the pseudo-ginseng seedlings is finished, selecting a microbial inoculum B, preparing a bacterial solution, connecting a medicine inlet and a watering opening, adjusting the size of the medicine inlet, opening a water pump, and spraying pseudo-ginseng; the prepared microbial inoculum B is applied once every 30-60 days, 4-6 times in the whole year and is continuously used for 2 years; the prepared fungicide B is applied by 400-500L per mu, and the irrigation quantity is 4-6 m3。
Example 13:
and (3) microbial inoculum C: consists of wood decay bacteria liquid, sugar and water;
and (3) microbial inoculum D: consists of wood decay bacteria liquid and water.
The preparation method of the microbial inoculum C comprises the following steps: measuring wood decay bacteria liquid according to the use amount of 3-4L/mu per mu, and diluting with clear water to obtain a diluent e; taking 8-12 kg of sugar, and dissolving with 10-20L of clear water to obtain a diluent f; mixing the diluent e and the diluent f, adding clear water for secondary dilution to 400-500L, and uniformly mixing for later use;
the preparation method of the microbial inoculum D comprises the following steps: measuring the wood decay bacteria liquid according to the use amount of 1-2L/mu per mu, diluting the wood decay bacteria liquid with clear water, diluting the wood decay bacteria liquid to 400-500L for the second time, and uniformly mixing the wood decay bacteria liquid for later use.
After the transplantation of the pseudo-ginseng seedlings is finished, uniformly spreading a layer of wood particles on the soil surface, and covering pine needles on the upper layer of the wood particles; after covering the pine needles, starting to apply the bacteria solution, selecting the microbial inoculum C for the first time, and spraying pseudo-ginseng by using an application machine; applying a second microbial inoculum after 7-10 days of interval, selecting microbial inoculum D for the second time, preparing a bacterial solution, and spraying pseudo-ginseng by using an application machine; after the second application, the prepared microbial inoculum D is applied once every 10-20 days, is used for 20-24 times every year and is continuously used for 2 years; chemical fertilizer is not added in the planting process, and the chemical pesticide input is reduced by half. The using amount of the wood particles is 1300-2600 kg/mu; the pine needle dosage is 1200-1800 kg/mu. 400-500L of the prepared fungicide C is applied to each mu; the prepared microbial inoculum D is applied by 300-400L per mu.
Example 14:
using the microbial inoculum prepared in example 13, part of the experimental data in the background of green panax notoginseng planting is as follows:
table 1 fertilizer input in the background of green panax notoginseng planting:
table 2 chemical input (input per acre per year) in the background of green panax notoginseng planting:
example 12:
using example 11, part of the data in the context of old landfills is as follows:
table 3 survival of panax notoginseng against old field multiple cropping management:
example 13:
using example 12, the partial experimental data below the organic panax notoginseng planting background is as follows:
table 4: fresh yield of panax notoginseng under the organic panax notoginseng planting background:
example 14:
and (3) microbial inoculum A: comprises bacillus subtilis, trichoderma harzianum, chitosan and water;
weighing bacillus subtilis and trichoderma harzianum according to the ratio of 1:1 by using the dosage of 600-1300 g/mu respectively per mu, and diluting with 5-10L of clear water to obtain a diluent a; measuring 1-2L of chitosan, and diluting with 5-10L of clear water to obtain a diluent b; mixing the diluent a and the diluent b, adding clear water for secondary dilution to 400-500L, and uniformly mixing for later use; the prepared microbial inoculum A is applied by 400-500L per mu, and the irrigation quantity is 4-6 m3。
And (3) bacterial agent B: consists of Trichoderma harzianum, Pseudomonas fluorescens and water;
the configuration method comprises the following steps: weighing Trichoderma harzianum and pseudomonas fluorescens at a ratio of 1:1 in an amount of 600-1300 g/mu respectively per mu, and diluting with 5-10L of clear water to obtain a diluent c; measuring 1-2L of chitosan, and diluting with 5-10L of clear water to obtain a diluent d; mixing the diluent c and the diluent d, adding clear water for secondary dilution to 400-500L, and uniformly mixing for later use; the prepared fungicide B is applied by 400-500L per mu, and the irrigation quantity is 4-6 m3。
The using method comprises the following steps: after the transplantation of the pseudo-ginseng seedlings is finished, firstly selecting a microbial inoculum A, preparing a bacterial liquid, connecting a medicine inlet and a watering opening, adjusting the size of the medicine inlet, opening a water pump, and spraying pseudo-ginseng; the prepared microbial inoculum B is applied once every 30-60 days, 4-6 times in the whole year and is continuously used for 1 year; in the next year, selecting the microbial inoculum B, preparing a bacterial solution, connecting a medicine inlet and a watering opening, adjusting the size of the medicine inlet, opening a water pump, and spraying pseudo-ginseng; the prepared microbial inoculum B is applied once every 30-60 days, 4-6 times in the whole year and is continuously used for 2 years.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. The microbial inoculum combination for planting pseudo-ginseng is characterized by comprising one or more of wood rot fungi, bacillus subtilis, pseudomonas fluorescens, trichoderma harzianum and chitosan.
2. The microbial inoculum combination for pseudo-ginseng planting according to claim 1, wherein the microbial inoculum combination comprises
A microbial inoculum A, a microbial inoculum B, a microbial inoculum C and a microbial inoculum D;
and (3) microbial inoculum A: comprises bacillus subtilis, trichoderma harzianum, chitosan and water;
and (3) bacterial agent B: consists of Trichoderma harzianum, Pseudomonas fluorescens and water;
and (3) microbial inoculum C: consists of wood decay bacteria liquid, sugar and water;
and (3) microbial inoculum D: consists of wood decay bacteria liquid and water.
3. The microbial inoculum combination for pseudo-ginseng planting according to claim 2, wherein the preparation method comprises the following steps:
the preparation method of the microbial inoculum A comprises the following steps: weighing bacillus subtilis and trichoderma harzianum according to the ratio of 1:1 by using the dosage of 600-1300 g/mu respectively per mu, and diluting with 5-10L of clear water to obtain a diluent a; measuring 1-2L of chitosan, and diluting with 5-10L of clear water to obtain a diluent b; mixing the diluent a and the diluent b, adding clear water for secondary dilution to 400-500L, and uniformly mixing for later use;
the preparation method of the microbial inoculum B comprises the following steps: weighing Trichoderma harzianum and pseudomonas fluorescens at a ratio of 1:1 in an amount of 600-1300 g/mu respectively per mu, and diluting with 5-10L of clear water to obtain a diluent c; measuring 1-2L of chitosan, and diluting with 5-10L of clear water to obtain a diluent d; mixing the diluent c and the diluent d, adding clear water for secondary dilution to 400-500L, and uniformly mixing for later use;
the preparation method of the microbial inoculum C comprises the following steps: measuring wood decay bacteria liquid according to the use amount of 3-4L/mu per mu, and diluting with clear water to obtain a diluent e; taking 8-12 kg of sugar, and dissolving with 10-20L of clear water to obtain a diluent f;
mixing the diluent e and the diluent f, adding clear water for secondary dilution to 400-500L, and uniformly mixing for later use;
the preparation method of the microbial inoculum D comprises the following steps: measuring the wood decay bacteria liquid according to the use amount of 1-2L/mu per mu, diluting the wood decay bacteria liquid with clear water, diluting the wood decay bacteria liquid to 400-500L for the second time, and uniformly mixing the wood decay bacteria liquid for later use.
4. The use method of the microbial inoculum combination for planting the pseudo-ginseng is characterized by comprising the following steps:
under the background of old land multiple cropping management, after the transplantation of pseudo-ginseng seedlings is completed, selecting a microbial inoculum A, preparing a bacterial solution, connecting a medicine inlet and a watering opening, adjusting the size of the medicine inlet, opening a water pump, and spraying pseudo-ginseng; the prepared microbial inoculum A is applied once every 30-60 days, 4-6 times in the whole year and is continuously used for 2 years;
under the background of organic panax notoginseng planting management, after the panax notoginseng seedlings are transplanted, selecting a microbial inoculum B, preparing a bacterial liquid, connecting a medicine inlet and a watering opening, adjusting the size of the medicine inlet, opening a water pump, and spraying panax notoginseng; the prepared microbial inoculum B is applied once every 30-60 days, 4-6 times in the whole year and is continuously used for 2 years;
under the background of green pseudo-ginseng planting management, after pseudo-ginseng seedlings are transplanted, uniformly spreading a layer of wood particles on the soil surface, and covering pine needles on the upper layer of the wood particles; after covering the pine needles, starting to apply the bacteria solution, selecting the microbial inoculum C for the first time, and spraying pseudo-ginseng by using an application machine; applying a second microbial inoculum after 7-10 days of interval, selecting microbial inoculum D for the second time, preparing a bacterial solution, and spraying pseudo-ginseng by using an application machine; after the second application, the prepared microbial inoculum D is applied once every 10-20 days, is used for 20-24 times every year and is continuously used for 2 years; chemical fertilizer is not added in the planting process, and the chemical pesticide input is reduced by half.
5. The use method of the microbial inoculum combination for pseudo-ginseng planting according to claim 4, wherein the temperature in the greenhouse is 18-25 ℃ when the microbial inoculum is used.
6. The use method of the microbial inoculum combination for pseudo-ginseng planting according to claim 4, wherein when the microbial inoculum is used, the soil humidity is controlled to be 50-70% and not more than 85%.
7. The use method of the microbial inoculum combination for pseudo-ginseng planting according to claim 4, wherein 400-500L of prepared microbial inoculum B is applied to each mu of land with the irrigation amount of 4-6 m under the background of organic pseudo-ginseng planting management3。
8. The use method of the microbial inoculum combination for pseudo-ginseng planting according to claim 4, wherein 400-500L of prepared microbial inoculum A is applied to each mu of land with the irrigation amount of 4-6 m under the background of old land multiple cropping management3。
9. The use method of the microbial inoculum combination for pseudo-ginseng planting according to claim 4, wherein in the background of green pseudo-ginseng planting management, the dosage of wood particles is 1300-2600 kg/mu; the pine needle dosage is 1200-1800 kg/mu.
10. The use method of the microbial inoculum combination for pseudo-ginseng planting according to claim 4, wherein 400-500L of the prepared microbial inoculum C is applied to each mu in the background of green pseudo-ginseng planting management; the prepared microbial inoculum D is applied by 300-400L per mu.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115191168A (en) * | 2022-08-05 | 2022-10-18 | 文山苗乡三七科技有限公司 | Method for restoring pseudo-ginseng continuous cropping soil biochar |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103125257A (en) * | 2013-03-26 | 2013-06-05 | 云南省农业科学院药用植物研究所 | Environment-friendly prevention and control method of panax notoginseng diseases |
| JP2013146255A (en) * | 2012-01-23 | 2013-08-01 | Mogami Ranen Kk | Mass propagation method of chrysanthemum pacificum, greening method using chrysanthemum pacificum, and cultivation method of wood rotting fungus used therefor |
| CN103392490A (en) * | 2013-08-14 | 2013-11-20 | 文山市苗乡三七实业有限公司 | Method for preventing pseudo-ginsheng root rot |
| CN106576866A (en) * | 2016-12-26 | 2017-04-26 | 昆明邹党农业科技有限公司 | Stereoscopic cultivation method of panax notoginseng |
| CN108887122A (en) * | 2018-07-27 | 2018-11-27 | 上海环垦生态科技股份有限公司 | Notoginseng planting method |
| CN108967095A (en) * | 2018-08-14 | 2018-12-11 | 文山市泽禄生物科技有限公司 | A kind of method that Radix Notoginseng is continuously cultivated |
| CN110012802A (en) * | 2017-09-11 | 2019-07-16 | 南阳中科国通生态科技有限公司 | A kind of continuous cropping implantation methods of Radix Notoginseng |
| CN110204050A (en) * | 2019-05-29 | 2019-09-06 | 农业农村部环境保护科研监测所 | The agricultural ecological intercepting system and method for preventing area source pollutants from spreading |
-
2021
- 2021-06-25 CN CN202110711272.2A patent/CN113273467B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2013146255A (en) * | 2012-01-23 | 2013-08-01 | Mogami Ranen Kk | Mass propagation method of chrysanthemum pacificum, greening method using chrysanthemum pacificum, and cultivation method of wood rotting fungus used therefor |
| CN103125257A (en) * | 2013-03-26 | 2013-06-05 | 云南省农业科学院药用植物研究所 | Environment-friendly prevention and control method of panax notoginseng diseases |
| CN103392490A (en) * | 2013-08-14 | 2013-11-20 | 文山市苗乡三七实业有限公司 | Method for preventing pseudo-ginsheng root rot |
| CN106576866A (en) * | 2016-12-26 | 2017-04-26 | 昆明邹党农业科技有限公司 | Stereoscopic cultivation method of panax notoginseng |
| CN110012802A (en) * | 2017-09-11 | 2019-07-16 | 南阳中科国通生态科技有限公司 | A kind of continuous cropping implantation methods of Radix Notoginseng |
| CN108887122A (en) * | 2018-07-27 | 2018-11-27 | 上海环垦生态科技股份有限公司 | Notoginseng planting method |
| CN108967095A (en) * | 2018-08-14 | 2018-12-11 | 文山市泽禄生物科技有限公司 | A kind of method that Radix Notoginseng is continuously cultivated |
| CN110204050A (en) * | 2019-05-29 | 2019-09-06 | 农业农村部环境保护科研监测所 | The agricultural ecological intercepting system and method for preventing area source pollutants from spreading |
Non-Patent Citations (2)
| Title |
|---|
| 于占湖;: "大型真菌多样性及在森林生态系统中的作用", 中国林副特产, no. 03, 15 June 2007 (2007-06-15), pages 81 - 85 * |
| 于占湖;: "大型真菌多样性及在森林生态系统中的作用", 中国林副特产, no. 03, pages 81 - 85 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115191168A (en) * | 2022-08-05 | 2022-10-18 | 文山苗乡三七科技有限公司 | Method for restoring pseudo-ginseng continuous cropping soil biochar |
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