CN109618852B - Propagation method of arbuscular mycorrhizal fungi and application thereof - Google Patents
Propagation method of arbuscular mycorrhizal fungi and application thereof Download PDFInfo
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Abstract
The invention discloses a propagation method of arbuscular mycorrhizal fungi and application thereof, wherein the propagation method comprises the following steps: mixing sandy soil and arbuscular mycorrhizal fungi to obtain mixed sandy soil, sowing clover seeds on the mixed sandy soil, irrigating with plant source tobacco water every 10 days from 3 months after sowing, and stopping irrigating from the fifth month until overground parts of the clovers wither. Then, the American ginseng seeds are treated by the high-concentration cluster-planted mycorrhizal fungi agent obtained by propagation, the seedlings are treated by the plant-derived tobacco water, the plant-derived tobacco water contains a signal substance for inducing the inoculation of the cluster-planted mycorrhizal fungi, and the agent obtained by propagation contains the high-concentration cluster-planted mycorrhizal fungi, so that the dip-dyeing rate of the cluster-planted mycorrhizal fungi in the American ginseng is greatly improved, the proportion of mycorrhizal symbionts is improved, the disease resistance and the survival rate of the American ginseng seedlings are improved, the use amount of chemical pesticides is reduced, and the yield and the quality of the American ginseng are improved.
Description
Technical Field
The invention relates to an expanding propagation method of arbuscular mycorrhizal fungi, and also relates to a method for improving the survival rate of American ginseng seedlings, belonging to the technical field of expanding propagation and application of arbuscular mycorrhizal fungi.
Background
Arbuscular Mycorrhizal Fungi (AMF) are obligate biotrophic fungi that can form symbiotic relationships with the roots of most higher plants. Arbuscular Mycorrhiza (AM) formed by symbiosis of plants and AMF has a structure and a function different from a simple plant root system, has important effects on plant growth, mineral metabolism, water absorption, resistance and yield and quality improvement, and especially has important significance in improving the transplanting survival rate and resistance in the seedling stage. At present, a plurality of reports related to plant cultivation using arbuscular mycorrhizal fungi, such as tobacco, traditional Chinese medicinal materials and the like, exist.
Patent 201310085299.0 discloses an application method of arbuscular mycorrhizal fungi in large-area tobacco cultivation, which comprises the steps of inoculating and propagating an initial arbuscular mycorrhizal fungi agent independently, then carrying out mixed inoculation on the arbuscular mycorrhizal fungi agent on tobacco by adopting a floating seedling raising method to obtain mycorrhizal tobacco seedlings, and directly applying the mycorrhizal tobacco seedlings to field production. Patent 201110366781.2 discloses a biological control method for improving disease resistance of cultivated traditional Chinese medicinal materials, which adopts Arbuscular Mycorrhizal Fungi (AMF) as biocontrol bacteria, and improves disease resistance of cultivated traditional Chinese medicinal materials by mycorrhizal seedling treatment before cultivation of the traditional Chinese medicinal materials. In the method, the AMF is contacted with the plant seeds or the root systems of seedlings for inoculation, the inoculation method easily causes the AMF to form a competitive relationship with indigenous bacteria in soil, the infection rate of the AMF is reduced, if the inoculation rate of the AMF is low, the proportion of the AMF is lower, the beneficial effects of mycorrhizal symbionts are less, and the promotion of growth effect of the AMF is directly influenced.
American ginseng is derived from Panax quinquefolium of AraliaceaePanax quinquefoliumThe dry root of L. belongs to common and rare medicinal materials, has cool property, sweet taste and slight bitter taste, and has the effects of tonifying qi and yin, clearing fire and relieving restlessness. Ginsenoside is the main effective component, and has strong anticancer and blood pressure lowering effectsAnd improving immunity. The pharmacological properties of replacing American ginseng for people who are not affected by the warm tonifying of ginseng, tonifying without dryness and the like enable the American ginseng to be widely used, and the economic value in the fields of medicines, foods, health care products, cosmetics and the like is gradually remarkable. American ginseng is successfully introduced in China in the 80 th of the 20 th century, and at present, China becomes the main producing area and the marketing big country of American ginseng. Shandong province is the main production area of American ginseng in China, the planting area reaches more than 70 percent of the whole country, 10000 more than one ton of fresh ginseng is produced every year, and the planting of American ginseng becomes an important support for regional economic development. However, American ginseng mainly adopts seeds or seedlings (1 year seedlings) as a main breeding mode, large-area seedling shortage is caused by serious diseases, poor resistance and the like of American ginseng seedlings in a seedling stage, and the direct economic loss caused by the seedling shortage is more than 30 percent every year. In order to prevent seedling shortage, ginseng farmers use a large amount of chemical pesticides, and the problems cause that the yield and the quality of American ginseng are greatly reduced, pesticide residues and heavy metals exceed standards, and the clinical medication safety of the ginseng is seriously threatened. The establishment of a high-quality American ginseng seedling breeding system by adopting effective measures is an important link for improving the yield and the quality of American ginseng medicinal materials.
Disclosure of Invention
The invention aims to provide an expanding propagation method of arbuscular mycorrhizal fungi, which is characterized in that during the expanding propagation process of the arbuscular mycorrhizal fungi, plant-derived tobacco water is used for processing, so that the infection rate and the infection strength of the arbuscular mycorrhizal fungi in a root system of clover are improved, the beneficial effects of mycorrhizal symbionts are exerted to a greater extent, and the method has the advantages of ecology, environmental protection, high inoculation rate and the like.
The plant source tobacco water is an aqueous solution formed by dissolving tobacco generated by stewing plant materials in water, the physiological and ecological effects of the plant source tobacco water become hot points of the international ecological community, the plant source tobacco water can effectively improve the germination rate of crop seeds, improve the vitality of seedlings and improve the accumulation of medicinal active substances in medicinal plants, and other effects are not reported at present. The inventor finds that the inoculation rate of the arbuscular mycorrhizal fungi can be improved to a great extent by adding a proper amount of plant-derived tobacco water in a proper time in the process of inoculating the Arbuscular Mycorrhizal Fungi (AMF), and the effect of the plant-derived tobacco water is not reported at present, so that the invention belongs to the initiative. Meanwhile, the high inoculation rate is more beneficial to the arbuscular mycorrhizal fungi accounting for more proportion, is beneficial to fully exerting the arbuscular mycorrhizal fungi to improve the photosynthesis and the nutrition metabolism of the plants and the resistance of the plants and better exerting the beneficial effects of mycorrhizal symbionts, so that the invention provides a new idea for the application of the arbuscular mycorrhizal fungi in plant cultivation.
The invention provides a propagation method of arbuscular mycorrhizal fungi, which comprises the following steps: mixing sandy soil and arbuscular mycorrhizal fungi to obtain mixed sandy soil, sowing clover seeds on the mixed sandy soil, irrigating with plant source tobacco water every 10 days from 3 months after sowing, and stopping irrigating from the fifth month until overground parts of the clovers wither.
In the present invention, the arbuscular mycorrhizal fungus used is preferably Endocalamus capsulatus (A)Glomus intraradices,G.I)。
In the invention, the preparation method of the plant source tobacco water comprises the following steps: firstly introducing smoke generated by 6-7kg of plant source smoldering for 50-60min into 500ml of cold water, and diluting the obtained water solution with water according to the volume ratio of 1:500 plus 5000 to obtain plant source smoke water; the plant source is a mixture of hawthorn branches and leaves, wild jujube branches and leaves and chinaberry branches and leaves in a mass ratio of 4-6:1-3: 1.
Further, the plant source tobacco water is prepared by the following method: airing the hawthorn branches and leaves, the wild jujube branches and leaves and the chinaberry branches and leaves, stacking the dried hawthorn branches and leaves, irradiating the dried hawthorn branches and leaves and the wild jujube branches and leaves and the chinaberry branches and leaves in a black room by using ultraviolet light for 20 to 24 hours, and then treating the mixture by using hot air at the temperature of between 80 and 90 ℃ for 7 to 8 hours; after treatment, mixing the hawthorn branches and leaves, the wild jujube branches and leaves and the chinaberry branches and leaves according to the mass ratio of 4-6:1-3:1, taking 6-7kg of the mixture as a plant source, introducing smoke generated by stewing 6-7kg of the plant source for 50-60min into 500ml of cold water, and diluting the obtained water solution with water according to the volume ratio of 1:500 plus 5000 to obtain the plant source smoke water.
Furthermore, the plant source tobacco water is poured from the 3 rd month after sowing, and the plant source tobacco water is stopped in the fifth month, and the tobacco water is used for 2 months. From the fifth month, neither tobacco water nor water was applied to the plant source, and the clovers were allowed to wither naturally.
Further, the sandy soil and the arbuscular mycorrhizal fungi are mixed according to the volume ratio of 8-12:1 to obtain mixed sandy soil.
Furthermore, in the propagation method, after the clover is sowed, besides the irrigation mode of the clover, the sowing mode and the management mode of the clover seeds are common modes in the prior art.
Further, within 2 months after sowing, watering is carried out once every 5 days.
Furthermore, watering and plant source smoke water each time, namely, sand fully absorbs moisture. Because the plant source tobacco water contains a large amount of water, watering is not needed after the plant source tobacco water is watered.
Furthermore, after the overground part of the clover withers, the lower part of the clover and the mixed sandy soil contain a large amount of arbuscular mycorrhizal fungi, and the lower part of the clover and the mixed sandy soil are the arbuscular mycorrhizal fungi after propagation. The method uses plant source tobacco water for treatment in the propagation expanding process, and stops irrigating after the plant source tobacco water is irrigated for a period of time, so that the clovers are in a water-deficient environment, the infection rate of arbuscular mycorrhizal fungi in the root systems of the clovers is improved, and the high-concentration arbuscular mycorrhizal fungi microbial inoculum is obtained.
The invention also provides a method for improving the survival rate of the American ginseng seedlings, which comprises the following steps:
(1) carrying out propagation on the arbuscular mycorrhizal fungi by adopting the propagation method of the arbuscular mycorrhizal fungi, and after the overground part withers, taking the rest mixed sandy soil and the lower part of the three-grassland as the propagated arbuscular mycorrhizal fungi;
(2) removing the aril of the American ginseng seeds, washing with water, burying in wet sand, refrigerating in the wet sand at-2-3 ℃, and performing wet sand lamination;
(3) soaking the treated American ginseng seeds in plant-derived tobacco water, mixing the soaked seeds with the propagated arbuscular mycorrhizal fungi, and sowing after mixing;
(4) and (3) spraying plant source tobacco water on the leaf surfaces every 10 days from the second month after sowing, stopping spraying the plant source tobacco water at the 5 th month, and performing normal management on the rest leaves until the American ginseng seedlings meet the requirements.
Further, in the step (3), the plant-derived tobacco water used is the plant-derived tobacco water used for propagation of the arbuscular mycorrhizal fungi. Generally, seeds of American ginseng are soaked in tobacco water of plant origin for 1-2 h.
Further, in the step (4), the seedlings are basically completely emerged in one month after sowing, and the plant source tobacco water is sprayed from the second month. Except for spraying plant source tobacco water regularly, the cultivation of the American ginseng is normally managed according to the conventional management mode of the prior art, for example, watering, fertilizing, deinsectization and the like are managed according to the normal conventional management mode.
Furthermore, the water is watered once every 5 days within one month after the sowing, and the water is thoroughly watered every time.
Further, plant source tobacco water is regularly sprayed on the American ginseng leaves, and the spraying is performed each time on the leaves with the liquid drops dropping. The plant source tobacco water is sprayed, and meanwhile, the plant source tobacco water is watered regularly according to the water demand condition of seedlings.
In the process of arbuscular mycorrhizal propagation, the clover is irrigated by using plant-derived tobacco water, so that the infection rate of arbuscular mycorrhizal fungi in the root system of the clover is improved, the infection strength and the arbuscular abundance of the arbuscular mycorrhizal fungi are improved, and the high-concentration arbuscular mycorrhizal fungi microbial inoculum is formed. Then, the American ginseng seeds are treated by the high-concentration cluster-planted mycorrhizal fungi agent obtained by propagation, the seedlings are treated by plant-derived tobacco water, the plant-derived tobacco water contains a signal substance for attracting the inoculation of the cluster-planted mycorrhizal fungi, and the high-concentration cluster-planted mycorrhizal fungi is contained in the agent obtained by propagation, so that the infection rate of the cluster-planted mycorrhizal fungi in the American ginseng is greatly improved, the proportion of mycorrhizal symbionts is improved, the disease resistance and the survival rate of the American ginseng seedlings are improved, the using amount of chemical pesticides is reduced, and the yield and the quality of the American ginseng are improved.
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FIG. 1 shows the infestation of arbuscular mycorrhizal fungi in the roots of American ginseng.
Detailed Description
The present invention is further illustrated by the following specific examples, which are intended to be purely exemplary and are not intended to be limiting.
In the following examples, Endocalamus nodosum (A)Glomus intraradices,G.I) Purchased from institute of plant nutrition and resources, academy of agriculture and forestry, Beijing.
Example 1
The plant source tobacco water of the invention can be prepared by adopting the device described in patent 201310631035.0, and the preparation method comprises the following steps:
taking hawthorn branches and leaves, wild jujube branches and leaves and chinaberry branches and leaves, airing, mixing according to a mass ratio of 5:2:1, stacking the mixture in a black room, irradiating for 24 hours by using ultraviolet light, and treating for 8 hours by using hot air at 80-90 ℃; after treatment, 6kg of mixture is taken as a plant source, the 6kg of plant source is ignited, oxygen supply is controlled after ignition so that the plant source is smoldered without generating open fire, and the plant source can be burned only by sparks, so that a large amount of smoke is generated. The smoke generated by braising for 60min is introduced into 500mL cold water for absorption, the obtained water solution is used as mother liquor, and the mother liquor is preserved at 4 ℃ in the dark for standby.
Example 2
Using a potting method with glomus intraradices (Glomus intraradices,G.I) As arbuscular mycorrhiza, carrying out propagation on the arbuscular mycorrhiza, and comprising the following steps of:
using clover to perform propagation, firstly, mixing sandy soil (sand to soil volume ratio =1:1, water content 60 wt%) and sacculus mildew in roots (G.I) And uniformly mixing according to the volume ratio of 10:1 to obtain the mixed sandy soil. Adding the mixed sandy soil into a flowerpot (diameter is 20 cm), then spreading clover seeds on the mixed sandy soil, covering the mixed sandy soil with the thickness of about 1cm, placing the mixture in a greenhouse, watering, and performing conventional management. And watering every 5 days within 2 months after sowing, and watering 50mL once. Watering 50mL of plant source tobacco water every 10 days from month 3, stopping watering the plant source tobacco water at the end of month 4, and not watering until the overground part withers. After the overground part withers, removing the overground part, and obtaining the underground part of the root system of the clover and the mixed sandy soil as the arbuscular mycorrhiza obtained by propagation.
The preparation method of the plant source tobacco water comprises the following steps: the mother liquor of example 1 was diluted with water at a volume ratio of 1:1000 to obtain a plant-derived liquid tobacco.
Example 3
Endocalamus capsulatus (2)Glomus intraradices,G.I) Propagation is carried out with the difference that: the preparation method of the plant source tobacco water comprises the following steps: the mother liquor of example 1 was diluted with water at a volume ratio of 1:500 to obtain a plant-derived liquid tobacco.
Example 4
Endocalamus capsulatus (2)Glomus intraradices,G.I) Propagation is carried out with the difference that: the preparation method of the plant source tobacco water comprises the following steps: the mother liquor obtained in example 1 was diluted with water at a volume ratio of 1:2000 to obtain a plant-derived liquid tobacco.
Example 5
Endocalamus capsulatus (2)Glomus intraradices,G.I) Propagation is carried out with the difference that: the preparation method of the plant source tobacco water comprises the following steps: the mother liquor of example 1 was diluted with water at a volume ratio of 1:4000 to obtain a plant-derived liquid tobacco.
Example 6
Endocalamus capsulatus (2)Glomus intraradices,G.I) Propagation is carried out with the difference that: the preparation method of the plant source tobacco water comprises the following steps: the mother liquor obtained in example 1 was diluted with water at a volume ratio of 1:5000 to obtain a plant-derived liquid tobacco.
Example 7
After the American ginseng seeds are harvested, the aril outside the American ginseng seeds are removed, the American ginseng seeds are washed by water, then the American ginseng seeds are mixed with wet sand with the water content of 60wt%, a layer of sand is formed, a layer of American ginseng seeds is placed in a cold storage room at the temperature of-2-3 ℃ for 3 months, and wet sand lamination treatment is carried out.
The mother liquor of example 1 was diluted with water at a volume ratio of 1:1000 to obtain a plant-derived liquid tobacco. The seeds after the wet sand stratification are taken out, soaked in plant source tobacco water for 2 hours, and then the seeds are propagated with the seeds obtained in the example 2 (Gliocladium incarnatum: (Glomus intraradices,G.I) Mixing and then sowing.
Watering once every 5 days after sowing until watering is thorough, spraying plant source smoke and water to leaves of American ginseng seedlings from 2 months after sowing once every 10 days until the leaves drip downwards, stopping spraying the plant source smoke and water in 5 months, and performing conventional management except spraying the plant source smoke and water.
Example 8
After the American ginseng seeds are harvested, the aril outside the American ginseng seeds are removed, the American ginseng seeds are washed by water, then the American ginseng seeds are mixed with wet sand with the water content of 60wt%, a layer of sand is formed, a layer of American ginseng seeds is placed in a cold storage room at the temperature of-2-3 ℃ for 3 months, and wet sand lamination treatment is carried out.
The mother liquor obtained in example 1 was diluted with water at a volume ratio of 1:5000 to obtain a plant-derived liquid tobacco. The seeds after the wet sand stratification are taken out, soaked in plant source tobacco water for 2 hours, and then the seeds are propagated with the seeds obtained in the example 2 (Gliocladium incarnatum: (Glomus intraradices,G.I) Mixing and then sowing.
Watering once every 5 days after sowing until watering is thorough, spraying plant source smoke and water to leaves of American ginseng seedlings from 2 months after sowing once every 10 days until the leaves drip downwards, stopping spraying the plant source smoke and water in 5 months, and performing conventional management except spraying the plant source smoke and water.
Example 9
American ginseng seedlings were cultured as in example 7, except that: and taking out the seeds after the wet sand stratification treatment, and soaking the seeds in the plant source tobacco water for 1 hour.
Example 10
American ginseng seedlings were cultured as in example 7, except that: the plant source tobacco water is prepared by the following method: the mother liquor of example 1 was diluted with water at a volume ratio of 1:500 to obtain a plant-derived liquid tobacco.
Example 11
American ginseng seedlings were cultured as in example 7, except that: the plant source tobacco water is prepared by the following method: the mother liquor obtained in example 1 was diluted with water at a volume ratio of 1:2000 to obtain a plant-derived liquid tobacco.
Comparative example 1
Using clover to perform propagation, firstly, mixing sandy soil (sand to soil volume ratio =1:1, water content 60 wt%) and sacculus mildew in roots (G.I) And uniformly mixing according to the volume ratio of 10:1 to obtain the mixed sandy soil. Adding the mixed sandy soil into a flowerpot (diameter is 20 cm), then spreading clover seeds on the mixed sandy soil, covering the mixed sandy soil with the thickness of about 1cm, placing the mixture in a greenhouse, watering, and performing conventional management. And watering every 5 days within 2 months after sowing, and watering 50mL once. Watering was started every 10 days from month 3 and stopped at the end of month 4 until the aerial parts withered. And after the overground part withers, obtaining the underground part of the clover root system and the mixed sandy soil, namely the AMF after propagation.
Comparative example 2
A mother liquor was prepared as in example 1, except that: the plant source is 6kg of Chinese scholartree, yellow rose and weeping willow with the mass ratio of 3:2: 1.
Endocalamus capsulatus (2)Glomus intraradices,G.I) Propagation is carried out with the difference that: the plant source liquid tobacco is prepared by diluting the mother liquor obtained from the Chinese scholartree, the yellow thorn rose and the weeping willow with water according to the volume ratio of 1: 1000.
Comparative example 3
Endocalamus capsulatus (2)Glomus intraradices,G.I) Propagation is carried out with the difference that: watering 50mL of the plant-derived tobacco water every 10 days from month 2 until the end of month 4, watering 50mL of the plant-derived tobacco water every 10 days from month five until the end of month six until the overground part withers.
Comparative example 4
After the American ginseng seeds are harvested, the aril outside the American ginseng seeds are removed, the American ginseng seeds are washed by water, then the American ginseng seeds are mixed with wet sand with the water content of 60wt%, a layer of sand is formed, a layer of American ginseng seeds is placed in a cold storage room at the temperature of-2-3 ℃ for 3 months, and wet sand lamination treatment is carried out.
The seeds after the wet sand stratification were taken out, soaked in water for 2 hours, and then mixed with the intraradicular glomus obtained after propagation of example 2, followed by sowing.
Watering once every 5 days after sowing until the water is completely watered, and meanwhile, carrying out conventional management.
Comparative example 5
American ginseng seedlings were cultured as in example 7, except that: the plant source liquid tobacco is prepared by diluting mother liquor obtained from Chinese scholartree, yellow thorn rose and weeping willow in a volume ratio of 1:1000 in comparative example 2.
Comparative example 6
After the American ginseng seeds are harvested, the aril outside the American ginseng seeds are removed, the American ginseng seeds are washed by water, then the American ginseng seeds are mixed with wet sand with the water content of 60wt%, a layer of sand is formed, a layer of American ginseng seeds is placed in a cold storage room at the temperature of-2-3 ℃ for 3 months, and wet sand lamination treatment is carried out.
The seeds after the wet sand stratification were taken out, the seeds were soaked in water for 2 hours, and then the seeds were mixed with the intraradicular glomus obtained after propagation of comparative example 1, followed by sowing.
Watering once every 5 days after sowing until the water is completely watered, and meanwhile, carrying out conventional management.
The infection conditions of the ascochyta radicis in clover and American ginseng seedlings are researched, and the method and the results are as follows:
1. after the overground part of the clover withers, taking the root system of the clover, detecting the mycorrhizal infection rate, mycorrhizal infection strength, mycorrhizal dependence and absolute arbuscular abundance of ascomycete in roots, wherein the detection method refers to a method recorded in Chinese arbuscular mycorrhizal fungi resource and germplasm resource (Wangshoushan, Zhang Shuban, Zhang Meiqing, Chinese arbuscular mycorrhizal fungi resource and germplasm resource, China agricultural publisher, 2012).
Mycorrhiza infection rate F% = (number of infected root segments/number of total root segments) × 100.
Mycorrhizal infection strength M% = (95n5+70n4+30n3+5n2+ n 1)/number of all root segments x 100; n5 represents: the number of roots infected by 5 grades is more than 90 percent; n4 represents: the number of root segments infected by 4 grades and the infection proportion are more than 50 percent; n3 represents: the number of 3-grade infected root segments, and the infection proportion is less than 50%; n2 represents: the number of 2-grade infected root segments, wherein the infection proportion is less than 10%; n1 represents: the number of roots infected by grade 1 is less than 1%.
Mycorrhiza dependence = (dry weight of inoculated plant-dry weight of missed plant)/dry weight of inoculated plant x 100.
Absolute arbuscular abundance a% = a × (M/100); a represents: the abundance of the shoots at the root segment of the infestation.
The impregnation of clover by the Endocalamus capsulatus after propagation in each example and comparative example is shown in Table 1 below:
2. after the American ginseng is sown for 5 and a half months, taking the root system of the American ginseng, and detecting the mycorrhizal infection rate, the mycorrhizal infection strength, the mycorrhizal dependence and the absolute arbuscular abundance of the intracapsular myceliophthora according to the same method. The infestation of arbuscular mycorrhizal fungi in the roots of American ginseng is shown in FIG. 1.
The conditions of the inroot capsular fungus staining of American ginseng for each example and comparative example are shown in Table 2 below:
Claims (7)
1. a method for propagating arbuscular mycorrhizal fungi is characterized by comprising the following steps: mixing sandy soil and arbuscular mycorrhizal fungi to obtain mixed sandy soil, then sowing clover seeds on the mixed sandy soil, irrigating by using plant source tobacco water every 10 days from 3 months after sowing, and stopping irrigating from the fifth month until overground parts of the clovers wither; the preparation method of the plant source tobacco water comprises the following steps: firstly introducing smoke generated by 6-7kg of plant source smoldering for 50-60min into 500ml of cold water, and diluting the obtained water solution with water according to the volume ratio of 1:500 plus 5000 to obtain plant source smoke water; the plant source is a mixture of hawthorn branches and leaves, wild jujube branches and leaves and chinaberry branches and leaves in a mass ratio of 4-6:1-3: 1.
2. The propagation method according to claim 1, wherein: mixing sandy soil and arbuscular mycorrhizal fungi according to the volume ratio of 8-12: 1.
3. The propagation method according to claim 1, wherein: watering every 5 days within 2 months after sowing.
4. The propagation method according to any one of claims 1 to 3, wherein: the arbuscular mycorrhizal fungus is Endocalamus capsulatus (A)Glomus intraradices,G.I)。
5. A method for improving the survival rate of American ginseng seedlings is characterized by comprising the following steps:
(1) expanding propagation of the arbuscular mycorrhizal fungi by using the method for expanding propagation of arbuscular mycorrhizal fungi according to any one of claims 1 to 4, wherein after the overground part withers, the rest mixed sandy soil and the lower part of the three-grassland are the arbuscular mycorrhizal fungi after expanding propagation;
(2) removing the aril of the American ginseng seeds, washing with water, burying in wet sand, refrigerating in the wet sand at-2-3 ℃, and performing wet sand lamination;
(3) soaking the treated American ginseng seeds in plant-derived tobacco water, mixing the soaked seeds with the propagated arbuscular mycorrhizal fungi, and sowing after mixing;
(4) and (3) spraying plant source tobacco water on the leaf surfaces every 10 days from the second month after sowing, stopping spraying the plant source tobacco water at the 5 th month, and performing normal management on the rest leaves until the American ginseng seedlings meet the requirements.
6. The method of claim 5, wherein: in the step (3), the American ginseng seeds are soaked in the plant-derived tobacco water for 1-2 h.
7. The method of claim 5, wherein: watering every 5 days after sowing.
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Effective date of registration: 20211117 Address after: 264400 East head of Tianmu Road, Zhangjiachan Town, Wendeng District, Weihai City, Shandong Province Patentee after: Weihai Wendeng Daodi ginseng industry development Co.,Ltd. Address before: 250022 No. 336, South Xin Zhuang West Road, Shizhong District, Ji'nan, Shandong Patentee before: University of Jinan |