CN108651195A - A method of improving the growth of Rhizoma Atractylodis Macrocephalae seedling and drought-resistant ability - Google Patents
A method of improving the growth of Rhizoma Atractylodis Macrocephalae seedling and drought-resistant ability Download PDFInfo
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- CN108651195A CN108651195A CN201810358302.4A CN201810358302A CN108651195A CN 108651195 A CN108651195 A CN 108651195A CN 201810358302 A CN201810358302 A CN 201810358302A CN 108651195 A CN108651195 A CN 108651195A
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- atractylodis macrocephalae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
Abstract
The present invention relates to a kind of raising Rhizoma Atractylodis Macrocephalae seedling growth and the methods of drought-resistant ability,Belong to the process field of Rhizoma Atractylodis Macrocephalae seedling,This method is by using Glomus mosseae as the first strain,Using Glomus etunicatum as the second strain,And it is mixed after carrying out the unique numerous work of expansion to the first strain and the second strain,Obtained arbuscular mycorrhizal fungi is expanded numerous mixture to apply in the Rhizoma Atractylodis Macrocephalae seedling medium added with dried poultrymanure,Atractylodes macrocephala seed is handled by using solution of cerium chloride by oxidation,And Rhizoma Atractylodis Macrocephalae seedling is sprayed using special spray water,To overcome in the prior art Rhizoma Atractylodis Macrocephalae mostly generation breeding after occur serious kind of sexual involution cause its resistance weaken,Quality declines,The high problem of the seedling death rate,And then significantly improve the growth ability and drought-resistant ability of Rhizoma Atractylodis Macrocephalae seedling,It is strong growth ability can be provided for Rhizoma Atractylodis Macrocephalae production,The strong seedling of drought-resistant ability,And then expand the development of Rhizoma Atractylodis Macrocephalae production,Expand the utilization of Rhizoma Atractylodis Macrocephalae resource.
Description
Technical field
The present invention relates to the processing technology field of Rhizoma Atractylodis Macrocephalae seedling more particularly to a kind of improving the growth of Rhizoma Atractylodis Macrocephalae seedling and drought-resistant ability
Method.
Background technology
Rhizoma Atractylodis Macrocephalae (scientific name：Atractylodes macrocephalaKoidz.)：Composite family [WTBX herbaceos perennial,
It is high 60 centimetres reachable, nodositas root-like stock.Stem is upright, whole Glabrous.Leaf alternate, blade pinniform are totally cleaved, and side sliver is draped over one's shoulders
Aciculiform, ellipse or oblong, Capping piece is bigger than side sliver, thin, the papery in whole food value of leaf ground, two sides green, hairless, cephaloid
Sequence list gives birth to stem branch top, bract green, and needle prick shape pinniform is totally cleaved.The wide mitriform of involucre, top aubergine.Achene inverted cone-shaped, 8-
October yields positive results.Ground, this kinds such as distribution Jiangsu Province, China, Zhejiang, Fujian, Jiangxi, Anhui, Sichuan, Hubei and Hunan have cultivation.
This kind also has numerous commercialization titles, such as according to the root-like stock shape of crude drug or crane shape art, gold thread art or Rhizoma Atractylodis Macrocephalae leg, by production
Ground is named, and such as emblem art, is named by root-like stock unearthed season, such as winter art.To be preferred in art (Zhejiang is in latent) quality.
The medical value of Rhizoma Atractylodis Macrocephalae has more than 2100 years long history, is common help class Chinese medicine, has invigorating the spleen benefit
The work(of gas, eliminate dampness and have diuretic effect, hidroschesis, tocolysis, custom have " north ginseng southern art ", " ten sides, nine art " to say.Rhizoma Atractylodis Macrocephalae principle active component is to wave
Hair oil and polysaccharide component, such as atractylone, atractylodes lactone, needle juniper ester and palmitic acid, modern research shows that Rhizoma Atractylodis Macrocephalae active ingredient is also
Immune function can be enhanced, have the effects that hypoglycemic, antibacterial anti-inflammatory, anti-aging and antitumor.
The sales volume of Rhizoma Atractylodis Macrocephalae occupies first of the large parts of generic medicinal plants in the whole nation in the past 10 years, and Rhizoma Atractylodis Macrocephalae annual requirement has reached at present
1.2 ten thousand tons or more.Domestic market Rhizoma Atractylodis Macrocephalae is mainly used for medicine materical crude slice and Chinese patent drug product, and Rhizoma Atractylodis Macrocephalae and its preparation are clinically extensive
Using.Rhizoma Atractylodis Macrocephalae is the key medicine invigorated the spleen and benefited qi, and clinical application mostly occurs with compound, some traditional prescription such as four gentleman of the tonifying Qi side head
Soup, invigorating the spleen will side seven taste Rhizoma Atractylodis Macrocephalaes dissipate, shenling baizhu powder, Rhizoma Atractylodis Macrocephalae is all important component therein.Rhizoma Atractylodis Macrocephalae has anti-aging, drop
Blood glucose, it is antitumor the effects that, so by external medicine, the extensive use of health products manufacturing enterprise.
In the course of cultivation, the breeding of Rhizoma Atractylodis Macrocephalae is mostly serious for being often resulted in after generative propagation always based on generative propagation
The problems such as kind sexual involution shows as resistance weakens, quality declines etc., and the seedling death rate is high, also seriously constrains Rhizoma Atractylodis Macrocephalae production
Development.As medicinal material demand constantly increases, the seedling problem of genunie medicinal materials Rhizoma Atractylodis Macrocephalae is more and more prominent, greatlys restrict Rhizoma Atractylodis Macrocephalae
The utilization of resource.
Invention content
In view of the above problems, the present invention provide it is a kind of raising Rhizoma Atractylodis Macrocephalae seedling growth and drought-resistant ability method, with gram
Clothes in the prior art Rhizoma Atractylodis Macrocephalae mostly generation breeding after occur serious kind of sexual involution cause its resistance weaken, quality decline, seedling it is dead
The high problem of rate is died, to significantly improve the growth ability and drought-resistant ability of Rhizoma Atractylodis Macrocephalae seedling, can be produced for Rhizoma Atractylodis Macrocephalae and growth energy is provided
The seedling that power is strong, drought-resistant ability is strong, and then the development of Rhizoma Atractylodis Macrocephalae production is expanded, expand the utilization of Rhizoma Atractylodis Macrocephalae resource.
To achieve the goals above, the technical solution that the present invention takes is：
A method of improving the growth of Rhizoma Atractylodis Macrocephalae seedling and drought-resistant ability, wherein including：
(1) the numerous matrix of expansion of arbuscular mycorrhizal fungi is configured
It is 2 by volume by turfy soil, river sand, humus：2：1 carries out being mixed to get substrate mixture, after crossing 20 mesh sieve,
It is sterilized and is dried, obtain the numerous matrix of expansion of arbuscular mycorrhizal fungi；
(2) expansion of arbuscular mycorrhizal fungi is numerous
The arbuscular mycorrhizal fungi expansion it is numerous include the first arbuscular mycorrhizal fungi numerous and the second arbuscular mycorrhizal fungi of expansion
Expand numerous；
a：The expansion of first arbuscular mycorrhizal fungi is numerous
1. using Glomus mosseae as the first strain, first bacterium of 20g is put into the numerous matrix of the expansion per 3kg
Kind, it is carried out disinfection 10-15min to butch clover and wheat seed with 10% hydrogen peroxide；
2. the numerous matrix of expansion is fitted into flowerpot, when the numerous matrix of expansion is filled to the 3/4 of flowerpot, by first bacterium
Kind is uniformly sprinkling upon in flowerpot, then the numerous matrix of expansion is filled flowerpot, then presses the butch clover and the wheat seed
According to 1:1 ratio uniform is sprinkling upon the numerous stromal surface of expansion, and one layer is covered in the butch clover and the wheat seed surface
It is described to expand numerous matrix, the expansion unabridged version body of the first arbuscular mycorrhizal fungi is obtained, is then irrigated；
3. using MS a great number of elements as nutrient solution, 1.65gNH is added in every liter of water4NO3、1.9g KNO3, 0.44g
CaCl2·2H2O、0.37g MgSO4·7H2O and 0.17g KH2PO4 obtains dilution water, dilutes the nutrient solution with dilution water
Spray water is obtained after 10 times；
4. the expansion unabridged version body that each week sprays first arbuscular mycorrhizal fungi using the spray water is primary, 3 months
Afterwards, the soil of the expansion unabridged version surface layer of first arbuscular mycorrhizal fungi, the soil of 3-10cm depths below surface layer and plant are harvested
The root system of plant is broken into root system of plant powder, the soil of the expansion unabridged version surface layer of the first arbuscular mycorrhizal fungi of mixing by root system
The soil and root system of plant powder of 3-10cm depths below earth, surface layer obtains and embraces son, Glomus mosseae rich in Glomus mosseae fungi
Mycelia, the numerous object of expansion for expanding numerous matrix and the first arbuscular mycorrhizal fungi of root system of plant；
b：The expansion of second arbuscular mycorrhizal fungi is numerous
1. using Glomus etunicatum as the second strain, second bacterium of 20g is put into the numerous matrix of the expansion per 3kg
Kind, it is carried out disinfection 10-15min to butch clover and wheat seed with 10% hydrogen peroxide；
2. the numerous matrix of expansion is fitted into flowerpot, when the numerous matrix of expansion is filled to the 3/4 of flowerpot, by second bacterium
Kind is uniformly sprinkling upon in flowerpot, then the numerous matrix of expansion is filled flowerpot, then presses the butch clover and the wheat seed
According to 1:1 ratio uniform is sprinkling upon the numerous stromal surface of expansion, and one layer is covered in the butch clover and the wheat seed surface
It is described to expand numerous matrix, the expansion unabridged version body of the second arbuscular mycorrhizal fungi is obtained, is then irrigated；
3. using MS a great number of elements as nutrient solution, 1.65gNH4NO3,1.9g KNO3,0.44g are added in every liter of water
CaCl22H2O, 0.37g MgSO47H2O and 0.17g KH2PO4 obtain dilution water, dilute the nutrition with dilution water
Spray water is obtained after 10 times of liquid；
4. the expansion unabridged version body that each week sprays second arbuscular mycorrhizal fungi using the spray water is primary, 3 months
Afterwards, the soil of the expansion unabridged version surface layer of second arbuscular mycorrhizal fungi, the soil of 3-10cm depths below surface layer and plant are harvested
The root system of plant is broken into root system of plant powder, the soil of the expansion unabridged version surface layer of the second arbuscular mycorrhizal fungi of mixing by root system
The soil and root system of plant powder of 3-10cm depths below earth, surface layer obtains and embraces son, Glomus etunicatum rich in Glomus etunicatum fungi
Mycelia, the numerous object of expansion for expanding numerous matrix and the second arbuscular mycorrhizal fungi of root system of plant；
(3) processing of Atractylodes macrocephala seed
Full Atractylodes macrocephala seed is selected, 2-4h is impregnated in the solution of cerium chloride by oxidation of 3-6mmol/L, later with clear water by Rhizoma Atractylodis Macrocephalae
Seed dries spare after rinsing 2 times；
(4) selection and sowing of Rhizoma Atractylodis Macrocephalae seedling medium are cultivated
1. choosing the good glutenite soil of loose permeability as Rhizoma Atractylodis Macrocephalae seedling medium, per basin, addition 50-100g is decomposed
Dried poultrymanure；
2. the Rhizoma Atractylodis Macrocephalae seedling medium is filled in flowerpot, when Rhizoma Atractylodis Macrocephalae seedling medium is filled to the 3/4 of flowerpot, by first clump
The numerous object of expansion of mycorrhizal fungi and the numerous object mixed in equal amounts of expansion of the second arbuscular mycorrhizal fungi obtain arbuscular mycorrhizal fungi and expand numerous mixing
Object, a basin expand numerous mixture using the arbuscular mycorrhizal fungi of 30-50g and are uniformly sprinkling upon in flowerpot, then by Rhizoma Atractylodis Macrocephalae seedling medium
Flowerpot is filled, the Atractylodes macrocephala seed that solution of cerium chloride by oxidation impregnated then is sprinkling upon Rhizoma Atractylodis Macrocephalae seedling medium surface, on Atractylodes macrocephala seed surface
One layer of Rhizoma Atractylodis Macrocephalae seedling medium is gently covered, is finally irrigated；
3. using MS a great number of elements as nutrient solution, 1.65gNH4NO3,1.9g KNO3,0.44g are added in every liter of water
CaCl22H2O, 0.37g MgSO47H2O and 0.17g KH2PO4 obtain dilution water, dilute the nutrition with dilution water
Spray water is obtained after 10 times of liquid；
4. after Atractylodes macrocephala seed germination and emergence, in the greenhouse by the flowerpot placement of the Atractylodes macrocephala seed with germination and emergence, when
When Rhizoma Atractylodis Macrocephalae seedling has 3-5 piece leaves, every 2 week sprays a Rhizoma Atractylodis Macrocephalae seedling using the spray water, is grown after 2 months
And the Rhizoma Atractylodis Macrocephalae seedling that drought-resistant ability is strong.
The above-mentioned method for improving the growth of Rhizoma Atractylodis Macrocephalae seedling and drought-resistant ability, wherein the process of disinfecting action is carried out in step (1)
It is：The substrate mixture after 20 mesh sieve will be crossed and be put into high-pressure sterilizing pot the 20min that sterilizes under conditions of 121 DEG C.
Above-mentioned technical proposal has the following advantages that or advantageous effect：
A kind of method improving the growth of Rhizoma Atractylodis Macrocephalae seedling and drought-resistant ability provided by the invention, by using Glomus mosseae conduct
First strain carries out the unique numerous work of expansion using Glomus etunicatum as the second strain, and to the first strain and the second strain
After mixed, obtained arbuscular mycorrhizal fungi is expanded into numerous mixture and is applied in the Rhizoma Atractylodis Macrocephalae seedling medium added with dried poultrymanure,
By using solution of cerium chloride by oxidation to handle Atractylodes macrocephala seed, and Rhizoma Atractylodis Macrocephalae seedling is sprayed using special spray water, it is existing to overcome
Having in technology Rhizoma Atractylodis Macrocephalae occur after mostly generation breeding, serious kind of sexual involution causes its resistance to weaken, quality declines, the seedling death rate
High problem, and then the growth ability and drought-resistant ability of Rhizoma Atractylodis Macrocephalae seedling are significantly improved, it can be produced for Rhizoma Atractylodis Macrocephalae and growth ability is provided
By force, the strong seedling of drought-resistant ability, and then the development of Rhizoma Atractylodis Macrocephalae production is expanded, expand the utilization of Rhizoma Atractylodis Macrocephalae resource.
Specific implementation mode
With reference to specific embodiment, the present invention is further illustrated, but not as limiting to the invention.
Embodiment 1：The selection of the numerous matrix of expansion of arbuscular mycorrhizal fungi
In order to select the numerous matrix of the expansion of best arbuscular mycorrhizal fungi, the present embodiment 1 to use turfy soil, river sand, corruption respectively
Grow matter and mixed-matrix (turfy soil, river sand, humus by volume 2：2：1 mixing) these four are as the first strain " Moses
Sacculus is mould " and the second strain " Glomus etunicatum " the numerous matrix of expansion, above-mentioned four kinds are expanded after numerous matrix cross 20 mesh sieve, and high pressure is put into
Sterilize in autoclave under the conditions of 121 DEG C 20min, thoroughly kills the arbuscular mycorrhizal fungi expanded in numerous matrix, four kinds of expansions after sterilizing
Numerous matrix is dried spare.
The expansion of arbuscular mycorrhizal fungi is numerous include the first arbuscular mycorrhizal fungi numerous and the second arbuscular mycorrhizal fungi the expansion of expansion it is numerous；
a：The expansion of first arbuscular mycorrhizal fungi is numerous
1. using Glomus mosseae as the first strain, the first strain of 20g is put into the numerous matrix of expansion per 3kg, with
10% hydrogen peroxide carries out disinfection 10-15min to butch clover and wheat seed；
2. numerous matrix will be expanded to be fitted into flowerpot, when the numerous matrix of expansion is filled to the 3/4 of flowerpot, the first strain is uniformly sprinkling upon flower
In basin, then numerous matrix will be expanded and fill flowerpot, then by butch clover and wheat seed according to 1:1 ratio uniform, which is sprinkling upon, expands numerous base
Matter surface covers one layer in butch clover and wheat seed surface and expands numerous matrix, obtains the expansion unabridged version of the first arbuscular mycorrhizal fungi
Body then irrigates；
3. using MS a great number of elements as nutrient solution, 1.65gNH4NO3,1.9g KNO3,0.44g are added in every liter of water
CaCl22H2O, 0.37gMgSO47H2O and 0.17g KH2PO4 obtain dilution water, dilute 10 times of nutrient solution with dilution water
After obtain spray water；
4. the expansion unabridged version body that each week sprays the first arbuscular mycorrhizal fungi using spray water is primary, after 3 months, harvest the
The soil and root system of plant of 3-10cm depths below the expansion soil of unabridged version surface layer of one arbuscular mycorrhizal fungi, surface layer, by plant roots
System is broken into root system of plant powder, 3-10cm below the soil of the expansion unabridged version surface layer of the first arbuscular mycorrhizal fungi of mixing, surface layer
Deep soil and root system of plant powder obtains and embraces son rich in Glomus mosseae fungi, Glomus mosseae mycelia, expands numerous matrix and plant
The numerous object of expansion of first arbuscular mycorrhizal fungi of object root system；
b：The expansion of second arbuscular mycorrhizal fungi is numerous
1. using Glomus etunicatum as the second strain, the second strain of 20g is put into the numerous matrix of expansion per 3kg, with
10% hydrogen peroxide carries out disinfection 10-15min to butch clover and wheat seed；
2. numerous matrix will be expanded to be fitted into flowerpot, when the numerous matrix of expansion is filled to the 3/4 of flowerpot, the second strain is uniformly sprinkling upon flower
In basin, then numerous matrix will be expanded and fill flowerpot, then by butch clover and wheat seed according to 1:1 ratio uniform, which is sprinkling upon, expands numerous base
Matter surface expands numerous matrix in butch clover and wheat seed surface covering one, obtains the expansion unabridged version body of the second arbuscular mycorrhizal fungi,
Then irrigate；
3. using MS a great number of elements as nutrient solution, 1.65gNH4NO3,1.9g KNO3,0.44g are added in every liter of water
CaCl22H2O, 0.37gMgSO47H2O and 0.17g KH2PO4 obtain dilution water, dilute 10 times of nutrient solution with dilution water
After obtain spray water；
4. the expansion unabridged version body that each week sprays the second arbuscular mycorrhizal fungi using spray water is primary, after 3 months, harvest the
The soil and root system of plant of 3-10cm depths below the expansion soil of unabridged version surface layer of two arbuscular mycorrhizal fungi, surface layer, by plant roots
System is broken into root system of plant powder, 3-10cm below the soil of the expansion unabridged version surface layer of the second arbuscular mycorrhizal fungi of mixing, surface layer
Deep soil and root system of plant powder obtains and embraces son rich in Glomus etunicatum fungi, Glomus etunicatum mycelia, expands numerous matrix and plant
The numerous object of expansion of second arbuscular mycorrhizal fungi of object root system.
Respectively to the spore count and four kind of second arbuscular mycorrhizal fungi in the numerous object of expansion of four kind of first arbuscular mycorrhizal fungi
The spore count expanded in numerous object carries out sediments microscope inspection, finds to use mixed-matrix (turfy soil：River sand：Humus volume ratio Wei 2：
2：1) expand numerous effect preferably (referring to table 1), Glomus mosseae is to have 134-153 spore in every gram of numerous object of expansion, expands numerous children
Mould every gram of set sacculus, which expands in numerous object, 94-118 spore.
Table 1：Difference expands numerous matrix and expands Glomus mosseae and Glomus etunicatum numerous influence
Different lowercase letter P in table 1<0.05 level difference is notable.
Embodiment 2：Glomus mosseae and Glomus etunicatum expand numerous host plant selection
Glomus mosseae and Glomus etunicatum are all arbuscular mycorrhizal fungi, need with root system of plant symbiosis, so selection close
Suitable host plant is numerous to the expansion of Glomus mosseae and Glomus etunicatum most important.Butch clover, wheat are chosen as host,
It chooses Glomus mosseae (Glomus mosseae) and Glomus etunicatum (Glomus ovata) is used as strain, select mixed-matrix
(turfy soil, river sand, humus by volume 2：2：1 mixing) the numerous matrix of expansion as Glomus mosseae and Glomus etunicatum, always
Meter needs 6 basins, and Glomus mosseae is used in three basins, and three basins in addition use Glomus etunicatum, and 20g bacterium are put in every basin 3kg matrix
Kind of (Glomus mosseae or Glomus etunicatum) carries out disinfection 15min to butch clover and wheat seed with 10% hydrogen peroxide, uses
Butch clover and wheat are individually sowed and butch clover and wheat 1：1 ratio mixed planting, relatively individually sowing and mixing are broadcast
Kind expands arbuscular mycorrhizal fungi numerous effect.The strain and host plant used in 6 basins combines as follows：
When the numerous matrix of expansion fills flowerpot 3/4, strain is uniformly sprinkling upon in flowerpot, then numerous matrix will be expanded and fill flowerpot, most
The butch clover and uniform wheat of independent sowing and mixed seeding are sprinkling upon afterwards and expand numerous stromal surface, will be planted with numerous matrix is expanded a little
Son gently cover, obtain the expansion unabridged version body of arbuscular mycorrhizal fungi, watering irrigates, in every liter of water be added 1.65g NH4NO3,
1.9g KNO3,0.44g CaCl22H2O, 0.37g MgSO47H2O and 0.17g KH2PO4 obtain dilution water, and use is dilute
Spray water is obtained after releasing water 10 times of nutrient solution of dilution, each week sprays primary using spray water.After 3 months, harvest clump branch
The soil and root system of plant of 3-10cm depths, root system of plant is smashed below the expansion soil of unabridged version surface layer of mycorrhizal fungi, surface layer
At root system of plant powder, mix the soil of the expansion unabridged version surface layer of arbuscular mycorrhizal fungi, 3-10cm depths below surface layer soil and
Root system of plant powder obtains and embraces son, mycelia, the numerous object of expansion for expanding numerous matrix and the arbuscular mycorrhizal fungi of root system of plant rich in fungi.
By sediments microscope inspection, it is found that butch clover and wheat miscegenation expand numerous Glomus mosseae and Glomus etunicatum effect
Preferably (referring to table 2), expand in the numerous object of expansion of numerous Glomus mosseae has 127-143 spore in every gram of numerous object of expansion, expands numerous children's set ball
There is 96-120 spore in every gram of numerous object of expansion in the numerous object of the mould expansion of capsule.
Table 2：Numerous influence is expanded Glomus mosseae and Glomus etunicatum in different hosts plant and combination
Different lowercase letter P in table 2<0.05 level difference is notable.
Embodiment 3：The influence that different chlorination cerium concentration dialogue art seeds are sprouted
Full Atractylodes macrocephala seed is selected, the solution of cerium chloride by oxidation of 1,3,6,9,12mmol/L is respectively configured, Atractylodes macrocephala seed is soaked
The 2h in the solution of cerium chloride by oxidation of various concentration is steeped, it is spare after later being rinsed Atractylodes macrocephala seed 2 times with clear water.Choose loose gas permeability
Glutenite soil that can be good is Rhizoma Atractylodis Macrocephalae seedling medium, the dried poultrymanure decomposed addition 50-100g per basin, when Rhizoma Atractylodis Macrocephalae seedling medium is filled
When flowerpot 3/4, numerous good Glomus mosseae and Glomus etunicatum strain mixed in equal amounts will be expanded, be uniformly sprinkling upon in flowerpot, per basin 30-
50g, then flowerpot is filled into Rhizoma Atractylodis Macrocephalae seedling medium, the Atractylodes macrocephala seed that earth solution impregnated finally is sprinkling upon stromal surface, with less
Perhaps Rhizoma Atractylodis Macrocephalae seedling medium gently covers seed, and watering irrigates, and germinating energy, the solution of cerium chloride by oxidation leaching of 3-6mmol/L are counted after 5d
The Atractylodes macrocephala seed germinating energy highest steeped, counts Rhizoma Atractylodis Macrocephalae germination rate after 15d, and the solution of cerium chloride by oxidation of 3-6mmol/L impregnated white
Art percentage of seedgermination highest, 92%-95%, higher than other processing (referring to table 3).
Table 3：Influence of the various concentration cerium chloride to Rhizoma Atractylodis Macrocephalae germination
| Earth solution processing | Germinating energy (after sowing 5d) % | Percentage of seedgermination (after sowing 10d) % |
| Distilled water | 24 | 75bc |
| The cerium chloride of 1mmol/L | 39 | 85ab |
| The cerium chloride of 3mmol/L | 41 | 92a |
| The cerium chloride of 6mmol/L | 42 | 95a |
| The cerium chloride of 9mmol/L | 30 | 80b |
| The cerium chloride of 12mmol/L | 30 | 70c |
Different lowercase letter P in table 3<0.05 level difference is notable.
Embodiment 4：The selection and sowing of Rhizoma Atractylodis Macrocephalae seedling medium are cultivated
For the nursery effect of more different arbuscular mycorrhizal fungi, we will expand numerous good Glomus mosseae and children's set sacculus
Mould species use individually and mixed in equal amounts uniformly uses afterwards.It is white to choose the good glutenite soil of loose permeability
Art seedling medium, the dried poultrymanure decomposed addition 50-100g per basin, when Rhizoma Atractylodis Macrocephalae seedling medium fills flowerpot 3/4, it is numerous good to expand
Strain is uniformly sprinkling upon in flowerpot, per basin 30-50g, then flowerpot is filled Rhizoma Atractylodis Macrocephalae seedling medium, was finally impregnated earth solution
Atractylodes macrocephala seed is sprinkling upon Rhizoma Atractylodis Macrocephalae seedling medium surface, is gently covered seed with a little Rhizoma Atractylodis Macrocephalae seedling medium, watering irrigates；Equal Rhizoma Atractylodis Macrocephalaes
After germination emergence, when seedling about 3-5 piece leaves, using MS a great number of elements as nutrient solution, 1.65g is added in every liter of water
NH4NO3,1.9g KNO3,0.44g CaCl22H2O, 0.37g MgSO47H2O and 0.17g KH2PO4 are diluted
Water obtains spray water after diluting 10 times of nutrient solution with dilution water, every 2 week sprayed using spray water primary.It is measured after 2 months
The aerial part and under ground portion fresh weight of Rhizoma Atractylodis Macrocephalae seedling, as shown in Table 4, what Glomus mosseae and Glomus etunicatum microbial inoculum were used in mixed way
The Rhizoma Atractylodis Macrocephalae root tuber mean fresh that seedling medium is cultivated is 1.1g, and aerial part fresh weight is about 13.5g, is significantly higher than control group and list
The processing solely used.
Table 4：The sowing influence of different arbuscular mycorrhizal fungi and its proportioning to Rhizoma Atractylodis Macrocephalae yield after two months
Different lowercase letter P in table 4<0.05 level difference is notable.
Embodiment 5：The measurement of Rhizoma Atractylodis Macrocephalae seedling drought tolerance
It is test material to choose 2 months big Rhizoma Atractylodis Macrocephalae seedling, and it is not use chlorination that control group and processing group, control group, which is arranged,
Cerium solution treatment crosses seed and without the Rhizoma Atractylodis Macrocephalae seedling using bush mycorrhizal fungi preparation nursery, and processing group is to use 3mmol/L
Cerium chloride processes seed and the Rhizoma Atractylodis Macrocephalae seedling using bush mycorrhizal fungi preparation nursery, is planted in greenhouse kind, continuous 20d is not poured
Water observes the survival rate of two processing Rhizoma Atractylodis Macrocephalae seedling, as shown in Table 5, the processing of solution of cerium chloride by oxidation and arbuscular mycorrhizal fungi combination
Rhizoma Atractylodis Macrocephalae seedling survival rate be 63%, be significantly higher than control.Restore after watering after normally watering, processing group has the life of 90% Rhizoma Atractylodis Macrocephalae seedling
It is long to restore normal, and after control group restores watering, only 40% Rhizoma Atractylodis Macrocephalae seedling restore normal growth.Simultaneously to the white of each processing
The arbuscular mycorrhizal fungi infection rate of art seedling root system is determined, and as a result shows what solution of cerium chloride by oxidation and arbuscular mycorrhizal fungi combined
The Rhizoma Atractylodis Macrocephalae seedling root invasion rate of processing is significantly higher than control group.
Table 5：The measurement of Rhizoma Atractylodis Macrocephalae seedling drought tolerance
Different lowercase letter P in table 5<0.05 level difference is notable.
Embodiment 6：
The embodiment of the present invention 6 provides a kind of method improving the growth of Rhizoma Atractylodis Macrocephalae seedling and drought-resistant ability, including：
(1) the numerous matrix of expansion of arbuscular mycorrhizal fungi is configured
It is 2 by volume by turfy soil, river sand, humus：2：1 carries out being mixed to get substrate mixture, after crossing 20 mesh sieve,
It is put into high-pressure sterilizing pot the 20min that sterilizes under conditions of 121 DEG C, is then dried, the numerous matrix of expansion of arbuscular mycorrhizal fungi is obtained；
(2) expansion of arbuscular mycorrhizal fungi is numerous
The expansion of arbuscular mycorrhizal fungi is numerous include the first arbuscular mycorrhizal fungi numerous and the second arbuscular mycorrhizal fungi the expansion of expansion it is numerous；
a：The expansion of first arbuscular mycorrhizal fungi is numerous
1. using Glomus mosseae as the first strain, the first strain of 20g is put into the numerous matrix of expansion per 3kg, with
10% hydrogen peroxide carries out disinfection 12min to butch clover and wheat seed；
2. numerous matrix will be expanded to be fitted into flowerpot, when the numerous matrix of expansion is filled to the 3/4 of flowerpot, the first strain is uniformly sprinkling upon flower
In basin, then numerous matrix will be expanded and fill flowerpot, then by butch clover and wheat seed according to 1:1 ratio uniform, which is sprinkling upon, expands numerous base
Matter surface covers one layer in butch clover and wheat seed surface and expands numerous matrix, obtains the expansion unabridged version of the first arbuscular mycorrhizal fungi
Body then irrigates；
3. using MS a great number of elements as nutrient solution, 1.65gNH is added in every liter of water4NO3、1.9g KNO3, 0.44g
CaCl2·2H2O、0.37g MgSO4·7H2O and 0.17g KH2PO4 obtains dilution water, dilutes 10 times of nutrient solution with dilution water
After obtain spray water；
4. the expansion unabridged version body that each week sprays the first arbuscular mycorrhizal fungi using spray water is primary, after 3 months, harvest the
The soil and root system of plant of 3-10cm depths below the expansion soil of unabridged version surface layer of one arbuscular mycorrhizal fungi, surface layer, by plant roots
System is broken into root system of plant powder, 3-10cm below the soil of the expansion unabridged version surface layer of the first arbuscular mycorrhizal fungi of mixing, surface layer
Deep soil and root system of plant powder obtains and embraces son rich in Glomus mosseae fungi, Glomus mosseae mycelia, expands numerous matrix and plant
The numerous object of expansion of first arbuscular mycorrhizal fungi of object root system；
b：The expansion of second arbuscular mycorrhizal fungi is numerous
1. using Glomus etunicatum as the second strain, second strain of 20g is put into the numerous matrix of expansion per 3kg,
It is carried out disinfection 12min to butch clover and wheat seed with 10% hydrogen peroxide；
2. numerous matrix will be expanded to be fitted into flowerpot, when the numerous matrix of expansion is filled to the 3/4 of flowerpot, the second strain is uniformly sprinkling upon flower
In basin, then numerous matrix will be expanded and fill flowerpot, then by butch clover and wheat seed according to 1:1 ratio uniform, which is sprinkling upon, expands numerous base
Matter surface covers one layer in butch clover and wheat seed surface and expands numerous matrix, obtains the expansion unabridged version of the second arbuscular mycorrhizal fungi
Body then irrigates；
3. using MS a great number of elements as nutrient solution, 1.65gNH4NO3,1.9g KNO3,0.44g are added in every liter of water
CaCl22H2O, 0.37g MgSO47H2O and 0.17g KH2PO4 obtain dilution water, dilute nutrient solution 10 with dilution water
Spray water is obtained after times；
4. the expansion unabridged version body that each week sprays the second arbuscular mycorrhizal fungi using spray water is primary, after 3 months, harvest the
The soil and root system of plant of 3-10cm depths below the expansion soil of unabridged version surface layer of two arbuscular mycorrhizal fungi, surface layer, by plant roots
System is broken into root system of plant powder, 3-10cm below the soil of the expansion unabridged version surface layer of the second arbuscular mycorrhizal fungi of mixing, surface layer
Deep soil and root system of plant powder obtains and embraces son rich in Glomus etunicatum fungi, Glomus etunicatum mycelia, expands numerous matrix and plant
The numerous object of expansion of second arbuscular mycorrhizal fungi of object root system；
The spore count of the numerous object of expansion of the numerous object of expansion and the second arbuscular mycorrhizal fungi to the first arbuscular mycorrhizal fungi of acquisition into
Row sediments microscope inspection, it is 2 to find to use by volume：2：1 turfy soil, the numerous effect of expansion of river sand and humus are preferable, expand numerous
Glomus mosseae be every gram and expand in numerous object and have 134-153 spore, expanding in every gram of numerous object of expansion of numerous Glomus etunicatum has 94-
118 spores.
(3) processing of Atractylodes macrocephala seed
Full Atractylodes macrocephala seed is selected, 2h is impregnated in the solution of cerium chloride by oxidation of 3mmol/L, later with clear water by Atractylodes macrocephala seed
It is dried after rinsing 2 times spare；
(4) selection and sowing of Rhizoma Atractylodis Macrocephalae seedling medium are cultivated
1. choosing the good glutenite soil of loose permeability as Rhizoma Atractylodis Macrocephalae seedling medium, per basin, addition 50-100g is decomposed
Dried poultrymanure；
2. Rhizoma Atractylodis Macrocephalae seedling medium is filled in flowerpot, when Rhizoma Atractylodis Macrocephalae seedling medium is filled to the 3/4 of flowerpot, by first clump of branch bacterium
The numerous object of expansion of mycorrhiza fungi and the numerous object mixed in equal amounts of expansion of the second arbuscular mycorrhizal fungi obtain arbuscular mycorrhizal fungi and expand numerous mixture, and one
Basin expands numerous mixture using the arbuscular mycorrhizal fungi of 30-50g and is uniformly sprinkling upon in flowerpot, then Rhizoma Atractylodis Macrocephalae seedling medium is filled flowerpot,
The Atractylodes macrocephala seed that solution of cerium chloride by oxidation impregnated then is sprinkling upon Rhizoma Atractylodis Macrocephalae seedling medium surface, one is gently covered on Atractylodes macrocephala seed surface
Layer Rhizoma Atractylodis Macrocephalae seedling medium, finally irrigates；
3. using MS a great number of elements as nutrient solution, 1.65gNH4NO3,1.9g KNO3,0.44g are added in every liter of water
CaCl22H2O, 0.37g MgSO47H2O and 0.17g KH2PO4 obtain dilution water, dilute nutrient solution 10 with dilution water
Spray water is obtained after times；
4. after Atractylodes macrocephala seed germination and emergence, in the greenhouse by the flowerpot placement of the Atractylodes macrocephala seed with germination and emergence, when
When Rhizoma Atractylodis Macrocephalae seedling has 3-5 piece leaves, every 2 week using spray water spray a Rhizoma Atractylodis Macrocephalae seedling, can be grown after 2 months and
The strong Rhizoma Atractylodis Macrocephalae seedling of drought-resistant ability.Rhizoma Atractylodis Macrocephalae seedling aerial part and under ground portion fresh weight, Glomus mosseae and children are measured after 2 months
The Rhizoma Atractylodis Macrocephalae root tuber mean fresh that the seedling medium that set sacculus mould agent is used in mixed way is cultivated is 1.1g, and aerial part fresh weight is about
13.5g。
In conclusion a kind of method improving the growth of Rhizoma Atractylodis Macrocephalae seedling and drought-resistant ability provided in an embodiment of the present invention, by adopting
Use Glomus mosseae as the first strain, using Glomus etunicatum as the second strain, and to the first strain and the second strain into
It is mixed after the unique numerous work of expansion of row, obtained arbuscular mycorrhizal fungi is expanded into numerous mixture and is applied added with dried poultrymanure
In Rhizoma Atractylodis Macrocephalae seedling medium, Atractylodes macrocephala seed is handled by using solution of cerium chloride by oxidation, and using special spray water sprinkling Rhizoma Atractylodis Macrocephalae children
Seedling, to overcome in the prior art Rhizoma Atractylodis Macrocephalae mostly generation breeding after occur serious kind of sexual involution cause its resistance weaken, quality
Decline, the problem that the seedling death rate is high, and then significantly improve the growth ability and drought-resistant ability of Rhizoma Atractylodis Macrocephalae seedling, can be that Rhizoma Atractylodis Macrocephalae is given birth to
Production provides the seedling that growth ability is strong, drought-resistant ability is strong, and then expands the development of Rhizoma Atractylodis Macrocephalae production, expands opening for Rhizoma Atractylodis Macrocephalae resource
Hair utilizes.
It should be appreciated by those skilled in the art that those skilled in the art combine the prior art and above-described embodiment can be real
The existing change case, it will not be described here.Such change case does not affect the essence of the present invention, and it will not be described here.
Presently preferred embodiments of the present invention is described above.It is to be appreciated that the invention is not limited in above-mentioned
Particular implementation；Any technical person familiar with the field makes many possible changes not departing from technical solution of the present invention
Dynamic and modification, or it is revised as the equivalent embodiment of equivalent variations, this is not affected the essence of the present invention.Therefore, it is every not
Be detached from technical solution of the present invention content, according to the technical essence of the invention it is made to the above embodiment it is any it is simple modification,
Equivalent variations and modification, still fall within technical solution of the present invention protection in the range of.
Claims (2)
1. a kind of method improving the growth of Rhizoma Atractylodis Macrocephalae seedling and drought-resistant ability, which is characterized in that including：
(1) the numerous matrix of expansion of arbuscular mycorrhizal fungi is configured
It is 2 by volume by turfy soil, river sand, humus：2：1 carries out being mixed to get substrate mixture, after crossing 20 mesh sieve, carries out
It sterilizes and dries, obtain the numerous matrix of expansion of arbuscular mycorrhizal fungi；
(2) expansion of arbuscular mycorrhizal fungi is numerous
The expansion of the arbuscular mycorrhizal fungi is numerous include the first arbuscular mycorrhizal fungi numerous and the second arbuscular mycorrhizal fungi the expansion of expansion it is numerous；
a：The expansion of first arbuscular mycorrhizal fungi is numerous
1. using Glomus mosseae as the first strain, first strain of 20g is put into the numerous matrix of the expansion per 3kg,
It is carried out disinfection 10-15min to butch clover and wheat seed with 10% hydrogen peroxide；
2. the numerous matrix of expansion is fitted into flowerpot, it is when the numerous matrix of expansion is filled to the 3/4 of flowerpot, first strain is equal
It is even to be sprinkling upon in flowerpot, then the numerous matrix of expansion is filled into flowerpot, then by the butch clover and the wheat seed according to 1:1
Ratio uniform be sprinkling upon it is described expand numerous stromal surface, cover one layer of expansion in the butch clover and the wheat seed surface
Numerous matrix obtains the expansion unabridged version body of the first arbuscular mycorrhizal fungi, then irrigates；
3. using MS a great number of elements as nutrient solution, 1.65g NH are added in every liter of water4NO3、1.9g KNO3, 0.44g CaCl2·
2H2O、0.37g MgSO4·7H2O and 0.17g KH2PO4 obtains dilution water, after diluting 10 times of the nutrient solution with dilution water
Obtain spray water；
4. the expansion unabridged version body that each week sprays first arbuscular mycorrhizal fungi using the spray water is primary, after 3 months, receive
Obtain the expansion soil of unabridged version surface layer of first arbuscular mycorrhizal fungi, 3-10cm depths below surface layer soil and root system of plant,
The root system of plant is broken into root system of plant powder, soil, the table of the expansion unabridged version surface layer of the first arbuscular mycorrhizal fungi of mixing
Layer or less 3-10cm depths soil and root system of plant powder, obtain rich in Glomus mosseae fungi embrace son, Glomus mosseae mycelia,
Expand the numerous object of expansion of numerous matrix and the first arbuscular mycorrhizal fungi of root system of plant；
b：The expansion of second arbuscular mycorrhizal fungi is numerous
1. using Glomus etunicatum as the second strain, second strain of 20g is put into the numerous matrix of the expansion per 3kg,
It is carried out disinfection 10-15min to butch clover and wheat seed with 10% hydrogen peroxide；
2. the numerous matrix of expansion is fitted into flowerpot, it is when the numerous matrix of expansion is filled to the 3/4 of flowerpot, second strain is equal
It is even to be sprinkling upon in flowerpot, then the numerous matrix of expansion is filled into flowerpot, then by the butch clover and the wheat seed according to 1:1
Ratio uniform be sprinkling upon it is described expand numerous stromal surface, cover one layer of expansion in the butch clover and the wheat seed surface
Numerous matrix obtains the expansion unabridged version body of the second arbuscular mycorrhizal fungi, then irrigates；
3. using MS a great number of elements as nutrient solution, 1.65g NH4NO3,1.9g KNO3,0.44g CaCl2 are added in every liter of water
2H2O, 0.37g MgSO47H2O and 0.17g KH2PO4 obtain dilution water, after diluting 10 times of the nutrient solution with dilution water
Obtain spray water；
4. the expansion unabridged version body that each week sprays second arbuscular mycorrhizal fungi using the spray water is primary, after 3 months, receive
Obtain the expansion soil of unabridged version surface layer of second arbuscular mycorrhizal fungi, 3-10cm depths below surface layer soil and root system of plant,
The root system of plant is broken into root system of plant powder, soil, the table of the expansion unabridged version surface layer of the second arbuscular mycorrhizal fungi of mixing
Layer or less 3-10cm depths soil and root system of plant powder, obtain rich in Glomus etunicatum fungi embrace son, Glomus etunicatum mycelia,
Expand the numerous object of expansion of numerous matrix and the second arbuscular mycorrhizal fungi of root system of plant；
(3) processing of Atractylodes macrocephala seed
Full Atractylodes macrocephala seed is selected, 2-4h is impregnated in the solution of cerium chloride by oxidation of 3-6mmol/L, later with clear water by Atractylodes macrocephala seed
It is dried after rinsing 2 times spare；
(4) selection and sowing of Rhizoma Atractylodis Macrocephalae seedling medium are cultivated
1. choosing the good glutenite soil of loose permeability as Rhizoma Atractylodis Macrocephalae seedling medium, per basin, addition 50-100g is decomposed does
Chicken manure；
2. the Rhizoma Atractylodis Macrocephalae seedling medium is filled in flowerpot, when Rhizoma Atractylodis Macrocephalae seedling medium is filled to the 3/4 of flowerpot, by first clump of branch bacterium
The numerous object of expansion of mycorrhiza fungi and the numerous object mixed in equal amounts of expansion of the second arbuscular mycorrhizal fungi obtain arbuscular mycorrhizal fungi and expand numerous mixture, and one
Basin expands numerous mixture using the arbuscular mycorrhizal fungi of 30-50g and is uniformly sprinkling upon in flowerpot, then Rhizoma Atractylodis Macrocephalae seedling medium is filled flower
The Atractylodes macrocephala seed that solution of cerium chloride by oxidation impregnated then is sprinkling upon Rhizoma Atractylodis Macrocephalae seedling medium surface by basin, is gently covered on Atractylodes macrocephala seed surface
One layer of Rhizoma Atractylodis Macrocephalae seedling medium of lid, finally irrigates；
3. using MS a great number of elements as nutrient solution, 1.65g NH4NO3,1.9g KNO3,0.44g CaCl2 are added in every liter of water
2H2O, 0.37g MgSO47H2O and 0.17g KH2PO4 obtain dilution water, after diluting 10 times of the nutrient solution with dilution water
Obtain spray water；
4. after Atractylodes macrocephala seed germination and emergence, the flowerpot of the Atractylodes macrocephala seed with germination and emergence is placed in the greenhouse, Rhizoma Atractylodis Macrocephalae is worked as
When seedling has 3-5 piece leaves, every 2 week sprays a Rhizoma Atractylodis Macrocephalae seedling using the spray water, is grown and is resisted after 2 months
The strong Rhizoma Atractylodis Macrocephalae seedling of non-irrigated ability.
2. as described in claim 1 improve Rhizoma Atractylodis Macrocephalae seedling growth and drought-resistant ability method, which is characterized in that in step (1) into
The process of row disinfecting action is：The substrate mixture after 20 mesh sieve will be crossed and be put into high-pressure sterilizing pot and gone out under conditions of 121 DEG C
Bacterium 20min.
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