CN103265345A - Method for regulating drought resistance of festuca arundinacea by sludge/rare-earth mixed seed soaking solution - Google Patents
Method for regulating drought resistance of festuca arundinacea by sludge/rare-earth mixed seed soaking solution Download PDFInfo
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Abstract
The invention discloses a method for regulating the maximum proline content by a sludge/rare-earth mixed seed soaking solution under the seed soaking concentration of a 300 cerium+sludge leaching solution. The sludge/rare-earth mixed seed soaking solution is composed of a sludge leaching solution and a rare-earth solution in a weight ratio of 1:1, wherein the sludge leaching solution is prepared by mixing sludge and distilled water in a weight ratio of 1:2, standing for 24 hours and filtering; and the rare-earth solution is a water solution containing 200-400 mg.L<-1> Ce(NO3)3.6H2O or La(NO3)3.6H2O. The experimental result indicates that as the drought stress intensity increases, the proline content in both the control plant and the treated plant respectively increases, and the proline content reaches the maximum value under the seed soaking concentration of the 300 cerium+sludge leaching solution. The invention provides a certain technical support for drought-resistant growth and maintenance management of lawn plants in arid areas.
Description
Technical field
The invention belongs to environmental protection technical field, relate to urban afforestation, particularly a kind of method that adopts mud rare earth mixing seed-soaking liquid regulation and control drought resistance of festuca arundinacea.
Background technology
Rare earth is described as " nutrient substance " and " growth regulator ", can promote growth and development of plants, improve crop yield, improve the activity of crop amylase, proteolytic enzyme, lipase, peroxidase, nitrate reductase and esterase isoreactivity and soil enzyme, enhance metabolism, illustrate that rare earth is a kind of novel plant-growth regulator.There are some researches show that an amount of rare earth element can promote plant-growth, improves seed vitality, promotes seed germination.Rare earth element (La, Ce, Nd, Sm etc.) and the title complex (Ln-Gly, Ln-Gly-VB6) thereof of executing an amount of concentration outward can strengthen the resistance of plant, alleviate environment stress to the injury of plant.Rare earth is exactly lanthanon in the periodic table of chemical element, 15 kinds of elements such as the quite similar lanthanum of its do as one likes matter, cerium, praseodymium, neodymium with to the very similar scandium of lanthanon character, yttrium totally 17 kinds elementary composition, be referred to as rare earth element, be called for short rare earth (RE or R).Rare earth also can be described as " benefit is planted element ".China has abundant rare earth Mineral resources, and minerogentic condition is superior, can be rated as richly endowed by nature.
The sewage disposal industry that China is present develop construction and running condition with Sewage Plant rapidly, can conservative estimation in nearly ten years, the mud generation will increase substantially, so sludge disposal will become outstanding more environmental problem of China.Sewage sludge is rich in organic and the useful source of nutrition of plant such as N, P, K.It can substitute fertilizer and improve crop yield.The growth of using in right amount plant has promoter action preferably, promotes economic benefit, increases economic benefit.Show that according to relevant report the sewage sludge of China nearly 45% is as agricultural use.The main characteristic of mud is the water ratio height, and organic content height, mud are by the solid organic matter that obtains in the sewage treatment process, and can special as fertilizer sources usually and other materials be used.
Lawn plant is the important composition of urban landscaping, and the lawn area coverage is one of important symbol of estimating the modern city its construction level.Urban ground whole available lawns except road, square cover, and it constitutes multi-level greening layout with arbor, shrub, draft flowers and trees etc., form green shade covering, height is straggly, flowers blooming like a piece of brocade graceful view.The effect of lawn plant in urban afforestation can be summarized as three major functions: i.e. natural functions, social function and beautifying functions.
The most general worldwide natural disaster that the arid problem is.According to statistics, global arid area accounts for 1/3rd of land area, and the trend that increases is year by year arranged.And China's area 53% all belong to annual precipitation less than the arid of 400mm, semiarid and ecological environment frailty is regional.The natural disaster that soil desertification wherein, soil erosion, sandstorm etc. cause because of arid is more and more serious, has jeopardized China's ecological environment security.Plant (as arid) under adverse circumstance can cause the increase of membrane damage and membrane permeability, and the accumulation of active oxygen radical, and the interior a series of antioxidant reductase of plant materials and small-molecule substance can be removed these active oxygen radicals, and namely plant is to the adaptation reaction of poor environment.After plant is subjected to drought stress, can produce a series of forms and biochemical reactions.On the form, along with the dehydration of plant materials, plant begins slowly to wilt, and blade or herbaceous plant over-ground part all can slowly become withered and yellow, and finally along with further arid, plant may be final dead.
Lawn plant plays a significantly greater role in the construction of urban green space, is bringing into play very important effect the greening of city neighborhood, public lawn, road bank protection, closed viewing and admiring aspect lawn and the soil conservation.In order to make the lawn plant healthy growth and to have the ultimate aim that good quality is turf management, its landscape effect, ornamental value and pay attention to for the mankind provide comfortable safe superiority such as motor activity place more and more to be subjected to people.
The production of China lawn plant at present exists problems, can't satisfy the needs of urban lawn planting, and wherein the germination rate of lawn plant and under adverse environmental factor existence be exactly an important limiting factor.In the production process of lawn, add mud vat liquor and rare earth element mixing seed soaking, mainly be to utilize mud vat liquor and rare earth element to have the effect of enhancing plant seed vigor, promotion plant-growth, enhancing stress resistance of plant, mud vat liquor and rare earth element are applied to lawn plant, improve the ability of the resistance of the vigor of lawn plant seed, the growth of accelerating lawn plant, raising lawn plant; And the application of this respect technology does not still have bibliographical information.Not only can widen the use approach of city sewage and sludge, can also reduce the production cost on lawn, be an economically viable approach.
The present invention discovers, the mud of handling has all been postponed leaf withering under water stress, tall fescue turf quality and root biomass have been improved, the use of data presentation biological sludge has changed the content of bio-hormone and tryptophane, and has improved the drought resistance of plant, and, contain some physiologically active substances in the mud, along with the increase of mud consumption, its effect is strengthened gradually, so resistance increases.Present technique research adopts mud and rare earth seed-soaking liquid to the Festuca Arundinacea processing of soaking seed, by index Changing Patterns such as the chlorophyllous content of Festuca Arundinacea, biomass, proline(Pro), mdas, analyze mud and the seed soaking of rare earth seed-soaking liquid and handle afterwards Festuca Arundinacea to the adaptive faculty of drought stress, for planting and the maintenance management of arid area lawn plant provides certain technical support.
Summary of the invention
The invention discloses a kind of mud rare earth seed-soaking liquid, it is characterized in that it is that mud vat liquor and the earth solution of 1:1 formed by parts by weight; Wherein said mud vat liquor refers to mud: the ratio of weight and number of distilled water is that 1:2 mixes back placement 24h filtration, obtains required mud vat liquor; It is 200-400 mg.L that earth solution refers to concentration
-1Ce (NO
3)
36H
2O or La (NO
3)
36H
2O solution.Ce (NO wherein
3)
36H
2The concentration of O earth solution is 200-400 mg.L
-1La (NO
3)
36H
2The concentration of O earth solution is 200-400 mg.L
-1
The present invention further discloses the application of mud rare earth seed-soaking liquid in preparation regulation and control proline content.
The present invention further discloses the method that adopts mud rare earth mixing seed-soaking liquid regulation and control drought resistance of festuca arundinacea, it is characterized in that being undertaken by following step:
(1) with the mud of fetching, air-dry under field conditions (factors), go the bulk particle standby with 2-4 mm sieve sieve;
(2) then with mud in distilled water with parts by weight be 1:2 be loaded in the container place 24h after, filter, obtain required mud vat liquor; Wherein contain Cr:0.5875 μ gl in the mud vat liquor
-1, Cu:3.3 μ g.l
-1, Pb:26.6 μ g.l
-1, Zn:36.9 μ g.l
-1, K:1754.9 μ g.l
-1, PH is 6.5-6.7.Soil organic matter content 4.68%, total nitrogen content 0.21%, available phosphorus 22.03 mg/kg, full potassium amount 324.3 mg/kg, pH 7.44, saturated aqueous rate 0.58 ml/g.
(3) respectively with distilled water with Ce (NO
3)
36H
2O, La (NO
3)
36H
2The O dissolving, and then make concentration 200-400 mg.L respectively
-1Earth solution standby;
(4) with the earth solution of above-mentioned mud vat liquor and the different concns mixed with 1:1, obtain mud and rare earth seed-soaking liquid;
(5) take by weighing full seed, uniform tall fescue seed 0.4g, place culture dish, do not have seed soaking 24h with above-mentioned solution respectively, evenly place the culture dish that the 100g garden mould is housed, at the sowing initial stage, fully water, the assurance seed can be sprouted and the seedling primary growth smoothly, after sprouting 5 days, the beginning drought stress, adopting weighs waters; The soil moisture content of garden mould should be the 35%-50% of saturation moisture capacity, be that the 100g soil moisture content should be 15.28-21.67g, coerce for moderate this moment, the heavy 37g of culture dish, if will keep moderate to coerce, then whole culture dish heavily is about 157g, guarantees that plant is in moderate and coerces process, remains on the 157g that weighs when namely water every day, be 40d experimental period, laboratory temperature is 23-27 ℃ between incubation period, and humidity is 45%-60%, and intensity of illumination is 470-750 μ molm
-2S
-1
(6) behind plant culturing 40 d, the neat root of Festuca Arundinacea is cradled, over-ground part is clean with distilled water flushing, and thieving paper blots surperficial moisture content, puts into baking oven, and 1 h that completes under 105 ℃ is dried to constant weight for 80 ℃, measures its over-ground part dry weight, is considered as biomass.
The more detailed preparation method of the present invention and test situation are as follows:
1 development materials and methods
1.1 experiment material
The mud that experiment is selected for use is taken from Jizhuangzi Waste Water Treatment Plant, Tianjin, and lawn plant is selected the common Festuca Arundinacea of northern China for use.Rare earth element is cerium and lanthanum, adopts triumphant Tonghua, Tianjin to learn the cerous nitrate that reagent company limited produces, and its molecular formula is: Ce (NO
3)
36H
2The lanthanum nitrate that O and Tianjin, Tianjin fine chemistry industry institute of section produce, its molecular formula is: La (NO
3)
36H
2O.Test used garden mould and pick up from the Tianjin Normal University campus, wherein, soil organic matter content 4.68%, total nitrogen content 0.21%, available phosphorus 22.03 mg/kg, full potassium amount 324.3 mg/kg, pH 7.44, saturated aqueous rate 0.58 ml/g.
The preparation of vat liquor: the mud of fetching, air-dry dirt is under field conditions (factors) removed the bulk particle with 2 mm sieves sieve.Then with mud: distilled water=1:2.Be loaded in the Erlenmeyer flask place 24 h after, filter then, obtain required mud vat liquor.
With distilled water with Ce (NO
3)
36H
2O and La (NO
3)
36H
2After the O dissolving, then the earth solution of above-mentioned mud vat liquor and different concns is obtained mud and rare earth seed-soaking liquid with the mixed of 1:1.
1.2 experimental design
8 processing are established in experiment altogether, are respectively pure distilled water, pure mud vat liquor, 200 mg.L
-1Cerium+mud vat liquor, 300 mg.L
-1Cerium+mud vat liquor, 400 mg.L
-1Cerium+mud vat liquor, 200 mg.L
-1Lanthanum+mud vat liquor, 300 mg.L-1 lanthanum+mud vat liquor, 400 mg.L
-1Lanthanum+mud vat liquor is contrast (CK) with distilled water, and all processing are three repetitions.Take by weighing full seed, uniform tall fescue seed 0.4g, place 5.5 cm culture dish, do not have seed soaking 24h with above-mentioned solution respectively, will soak good seed then and remove surperficial moisture content with the filter paper suction, evenly place the culture dish of the diameter 9cm that 100 g garden moulds are housed, add water to native surface wettability.The sowing initial stage, fully water, the assurance seed can be sprouted and the seedling primary growth smoothly.After sprouting 5 days, the beginning drought stress, adopting weighs waters.
The soil moisture content of garden mould should be the 35%-50% of saturation moisture capacity.Be that the 100g soil moisture content should be 15.28-21.67g, coerce for moderate this moment, the heavy 37g of culture dish.If will keep moderate to coerce, then whole culture dish heavily is about 157 g.Guarantee that plant is in moderate and coerces process, remain on when namely water every day and weigh about 157 g.Be 40 d experimental period, and laboratory temperature is 23-27 ℃ between incubation period, and humidity is 45%-60%, and intensity of illumination is 470-750 μ molm
-2S
-1
1.3 index determining method
1.3.1 the mensuration of plant height and biomass
Behind plant culturing 40 d, the neat root of Festuca Arundinacea is cradled, over-ground part is clean with distilled water flushing, and thieving paper blots surperficial moisture content, puts into baking oven, and 1 h that completes under 105 ℃ is dried to constant weight for 80 ℃, measures its over-ground part dry weight, is considered as biomass.
1.3.2 the mensuration of chlorophyll content
The mensuration of chlorophyll content adopts acetone-alcohol mixeding liquid method: accurately takes by weighing 0.1 g, shreds, put into the little black box of experiment, add acetone-ethanol (1:1) mixed extract 10 mL, and capping plug, at room temperature 24 h are placed in the dark place, bleach up to material.Under 663 nm and 645 nm wavelength, measure optical density(OD) then.Calculate according to formula.
Chl a=12.71A663-2.59A645 Chl b=22.88A645-4.67A663
Chl a+b= Chl a + Chl b=8.04 A663+20.29 A645
Leaf green cubage formula (mgg
-1)=
1.3.3 the mensuration of mda content
Take by weighing 0.5 g blade, extract constant volume 10 ml, 4000 r.min with 10%TCA
-1Centrifugal 10 min get the 0.6%TBA that liquid 2 ml in upper strata add 2 ml again, 15min in the boiling water, and centrifugal 10 min get supernatant liquor and survey light absorption value under 532 nm and 450 nm.
1.3.4 the mensuration of relative conductivity
Relative conductivity adopts the conductivitimeter method to measure: the deionized water of 10 ml is put into numbered test tube, measure conductivity value I
0Each sample takes by weighing blade 0.2 g, is cut into 0.5 cm
2Fritter place test tube, accurately add deionized water 10 ml, soak 24 h, measure specific conductivity with DDS-11D direct-reading type conductivity meter and be measured as I
1After the mensuration test tube is placed on boiling water bath 0.5 h, kill tissues is surveyed I under room temperature
2Calculate relative conductivity (%)=(I
1-I
0)/(I
2-I
0) * 100%.
1.3.5 the mensuration of proline content
Take by weighing 0.5 g blade, shred, place Boiling tube respectively, add the sulphosalicylic acid of 10 ml 3% then respectively, boiling water extraction 10 min, 2 ml are drawn in another clean test tube with ground stopper in the cooling back, add 2 ml Glacial acetic acid and 2 ml acid ninhydrines, heating 30 min in the boiling water, cooling, with 4 ml methylbenzene extraction, centrifugal 5 min of 3000 rmin-1 get upper strata liquid and survey light absorption value under 520 nm.
1.4 data processing
Data analysis adopts EXCEL and SPSS17.0 analysis software to handle
2 development results and analysis
2.1 mud and the seed soaking of rare earth mixed solution are to the influence of Festuca Arundinacea plant height and biomass under the drought stress
By table 1 as seen, during the drought stress, the Festuca Arundinacea plant height of mixed solution seed soaking illustrates that apparently higher than contrast the mixed solution seed soaking obviously helps to alleviate the influence of plant drought stress.Wherein in the cerium mixed solution seed soaking, 300 ceriums+mud vat liquor exceeds 10.0%, 9.6%, 8.7% than contrast respectively at after planting 10d, 20d, 25d, with the contrast differences significantly (
P﹤ 0.05).For lanthanum mixed solution seed soaking, except 200 lanthanums are handled the plant height of 5d, other plant height of handling each minute all be significantly higher than contrast (
P﹤ 0.05), wherein, 400 lanthanums+mud vat liquor exceeds 53.0% in 5d ratio contrast after planting.
By table 2 as seen, the mixed solution seed soaking is to the dry weight fresh weight obvious effect of Festuca Arundinacea, and especially the high concentration rare earth element has significantly improved the biomass of Festuca Arundinacea under the drought stress.300 ceriums in the fresh weight+mud vat liquor is that 56.22%, the 400 lanthanum+mud vat liquor of contrast is 58.5% of contrast.300 ceriums in the dry weight+mud vat liquor is that 156.22%, the 400 lanthanum+mud vat liquor of contrast is 158.52% of contrast.
Table 1 mud and rare earth mixed solution are to the influence (cm) of Festuca Arundinacea plant height under the drought stress
Table 2 mud and rare earth mixed solution are to the influence of Festuca Arundinacea ground biomass under the drought stress
| Concentration (mgL-1) | Fresh weight (g/ ware) | Dry weight (g/ ware) | Fresh and dried ratio |
| CK | 2.17±0.36b | 0.14±0.04b | 0.06±0.01b |
| Pure mud vat liquor | 2.64±0.20ab | 0.23±0.02ab | 0.09±0.01ab |
| 200 ceriums+mud vat liquor | 2.18±0.45b | 0.21±0.02ab | 0.10±0.02a |
| 300 ceriums+mud vat liquor | 3.39±0.03a | 0.29±0.02a | 0.09±0.01ab |
| 400 ceriums+mud vat liquor | 3.18±0.09a | 0.28±0.02a | 0.09±0.01ab |
| CK | 2.17±0.36b | 0.14±0.04b | 0.06±0.01a |
| Pure mud vat liquor | 2.64±0.20ab | 0.23±0.02ab | 0.09±0.01a |
| 200 lanthanums+mud vat liquor | 3.07±0.26ab | 0.24±0.02ab | 0.08±0.01a |
| 300 lanthanums+mud vat liquor | 3.43±0.51a | 0.28±0.08ab | 0.08±0.01a |
| 400 lanthanums+mud vat liquor | 3.44±0.07a | 0.30±0.04a | 0.09±0.01a |
2.2 mud and the seed soaking of rare earth mixed solution are to the influence of Festuca Arundinacea chlorophyll content under the drought stress
Chlorophyll content and leave photosynthetic ability size are closely related, and drought stress can cause the chlorophyll content of Festuca Arundinacea to reduce, add lanthanum after drought stress Festuca Arundinacea chlorophyll a content all be higher than contrast, but difference not significantly (
P0.05) (seeing Table 3).Drought stress reduces the photosynthesis ability of plant by all means.As can be seen from Table 4, during the drought stress, the chlorophyll content of each treatment group contrasts the rising that all has in various degree.And the cerium mixed solution handle in total chlorophyll content of 200 ceriums+mud vat liquor reach significant difference (
P﹤ 0.05), exceed 152.13% than contrast.200 lanthanums in handling for the lanthanum mixed solution ++ total chlorophyll content of mud vat liquor reach significant difference (
P﹤ 0.05), be 1.27 times that contrast.
Table 3 mud and rare earth mixed solution are to the influence (mgg of Festuca Arundinacea chlorophyll content under the drought stress
-1FW)
2.3 mud and the seed soaking of rare earth mixed solution are to the influence of Festuca Arundinacea mda, proline(Pro) and relative conductivity under the drought stress
The mixed solution seed soaking sees Table 4 to the influence of Festuca Arundinacea MDA content under the drought stress.The result shows: the Festuca Arundinacea of 300 ceriums+mud vat liquor and 300 lanthanums+mud vat liquor reaches maximum difference, has descended 35.79% and 34.74% than contrast respectively.As can be seen from Table 5, there were significant differences compared with the control (P ﹤ 0.05) for each proline content of handling, and wherein, 300 ceriums+mud vat liquor is 1.90 times of control group.Test-results shows that during drought stress, the specific conductivity of each treatment group descends compared with the control to some extent.300 ceriums+mud vat liquor compared with the control, difference has reached conspicuous level, descends 33.33% than contrast.For the lanthanum mixed solution, significant difference shows in 300 lanthanums+mud vat liquor, reduction amplitude maximum in managing throughout than contrast decline 22.22% its specific conductivity.
Table 4 mud and rare earth mixed solution are to the influence of Festuca Arundinacea mda, proline(Pro) and relative conductivity under the drought stress
3 development conclusions
In this research, along with the increase of drought stress intensity, adjoining tree all presents ascendant trend with the proline content of handling plant, and the content of proline(Pro) is the highest with content under the seed soaking concentration of 300 ceriums+mud vat liquor.
Embodiment:
The present invention will be further described below in conjunction with specific embodiment, and following each embodiment only is used for explanation the present invention and is not limitation of the present invention.
Embodiment 1
A kind of mud rare earth seed-soaking liquid, it is that mud vat liquor and the earth solution of 1:1 formed by parts by weight; Wherein said mud vat liquor refers to mud: the ratio of weight and number of distilled water is that 1:2 mixes back placement 24h filtration, obtains required mud vat liquor; Earth solution refers to Ce (NO
3)
36H
2O or La (NO
3)
36H
2The O aqueous solution.
Ce (NO wherein
3)
36H
2The concentration of O earth solution is 200,300,400 mg.L
-1
La (NO
3)
36H
2The concentration of O earth solution is 200,300,400 mg.L
-1
Regulate and control method:
(1) with the mud of fetching, air-dry under field conditions (factors), go the bulk particle standby with 2 mm sieves sieve;
(2) then with mud in distilled water with parts by weight be 1:2 be loaded in the container place 24h after, filter, obtain required mud vat liquor;
(3) respectively with distilled water with Ce (NO
3)
36H
2O, La (NO
3)
36H
2The O dissolving, and then make concentration 200-400 mg.L respectively
-1Earth solution standby;
(4) with the earth solution of above-mentioned mud vat liquor and the different concns mixed with 1:1, obtain mud and rare earth seed-soaking liquid;
(5) take by weighing full seed, uniform tall fescue seed 0.4g, place 5.5 cm culture dish, do not have seed soaking 24h with above-mentioned solution respectively, to soak good seed then and remove surperficial moisture content with the filter paper suction, evenly place the culture dish of the diameter 9cm that 100 g garden moulds are housed, add water to native surface wettability.The sowing initial stage, fully water, the assurance seed can be sprouted and the seedling primary growth smoothly.After sprouting 5 days, the beginning drought stress, adopting weighs waters.The soil moisture content of garden mould should be 50% of saturation moisture capacity.Be that the 100g soil moisture content should be 15.28-21.67g, coerce for moderate this moment, the heavy 37g of culture dish.If will keep moderate to coerce, then whole culture dish heavily is about 157 g.Guarantee that plant is in moderate and coerces process, remain on when namely water every day and weigh about 157 g.Be 40 d experimental period, and laboratory temperature is 23 ℃ between incubation period, and humidity is 45%, and intensity of illumination is 500 μ molm
-2S
-1
(6) behind plant culturing 40 d, the neat root of Festuca Arundinacea is cradled, over-ground part is clean with distilled water flushing, and thieving paper blots surperficial moisture content, puts into baking oven, and 1 h that completes under 105 ℃ is dried to constant weight for 80 ℃, measures its over-ground part dry weight, is considered as biomass.
Claims (5)
1. mud rare earth seed-soaking liquid is characterized in that it is that mud vat liquor and the earth solution of 1:1 formed by parts by weight; Wherein said mud vat liquor refers to mud: the ratio of weight and number of distilled water is that 1:2 mixes back placement 24h filtration, obtains required mud vat liquor; Earth solution refers to Ce (NO
3)
36H
2O or La (NO
3)
36H
2The O aqueous solution.
2. the described mud rare earth of claim 1 seed-soaking liquid, wherein Ce (NO
3)
36H
2The concentration of O earth solution is 200-400 mg.L
-1
3. the described mud rare earth of claim 1 seed-soaking liquid, wherein La (NO
3)
36H
2The concentration of O earth solution is 200-400 mg.L
-1
4. the application of the described mud rare earth of claim 1 seed-soaking liquid in preparation regulation and control proline content.
One kind adopt mud rare earth mixing seed-soaking liquid regulation and control drought resistance of festuca arundinacea method, it is characterized in that being undertaken by following step:
(1) with the mud of fetching, air-dry under field conditions (factors), go the bulk particle standby with 2-4 mm sieve sieve;
(2) then with mud in distilled water with parts by weight be 1:2 be loaded in the container place 24h after, filter, obtain required mud vat liquor;
(3) respectively with distilled water with Ce (NO
3)
36H
2O, La (NO
3)
36H
2The O dissolving, and then make concentration 200-400 mg.L respectively
-1Earth solution standby;
(4) with the earth solution of above-mentioned mud vat liquor and the different concns mixed with 1:1, obtain mud and rare earth seed-soaking liquid;
(5) take by weighing full seed, uniform tall fescue seed 0.4g, place culture dish, do not have seed soaking 24h with above-mentioned solution respectively, evenly place the culture dish that the 100g garden mould is housed, at the sowing initial stage, fully water, the assurance seed can be sprouted and the seedling primary growth smoothly, after sprouting 5 days, the beginning drought stress, adopting weighs waters; The soil moisture content of garden mould should be the 35%-50% of saturation moisture capacity, be that the 100g soil moisture content should be 15.28-21.67g, coerce for moderate this moment, the heavy 37g of culture dish, if will keep moderate to coerce, then whole culture dish heavily is about 157g, guarantees that plant is in moderate and coerces process, remains on 157 g that weigh when namely water every day, be 40 d experimental period, laboratory temperature is 23-27 ℃ between incubation period, and humidity is 45%-60%, and intensity of illumination is 470-750 μ molm
-2S
-1
(6) behind plant culturing 40 d, the neat root of Festuca Arundinacea is cradled, over-ground part is clean with distilled water flushing, and thieving paper blots surperficial moisture content, puts into baking oven, and 1 h that completes under 105 ℃ is dried to constant weight for 80 ℃, measures its over-ground part dry weight.
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| CN108651195A (en) * | 2018-04-20 | 2018-10-16 | 绍兴文理学院 | A method of improving the growth of Rhizoma Atractylodis Macrocephalae seedling and drought-resistant ability |
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| CN108651195B (en) * | 2018-04-20 | 2020-08-07 | 绍兴文理学院 | Method for improving growth and drought resistance of bighead atractylodes rhizome seedlings |
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