CN102612946A - Method for increasing photosynthetic capacity of drought mixed matrix lawn plants by adopting rare-earth cerium - Google Patents
Method for increasing photosynthetic capacity of drought mixed matrix lawn plants by adopting rare-earth cerium Download PDFInfo
- Publication number
- CN102612946A CN102612946A CN2012100811498A CN201210081149A CN102612946A CN 102612946 A CN102612946 A CN 102612946A CN 2012100811498 A CN2012100811498 A CN 2012100811498A CN 201210081149 A CN201210081149 A CN 201210081149A CN 102612946 A CN102612946 A CN 102612946A
- Authority
- CN
- China
- Prior art keywords
- cerium
- rare earth
- mgkg
- under
- earth cerium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to a method for increasing the photosynthetic capacity of drought mixed matrix lawn plants by adopting rare-earth cerium. The method comprises the step of sowing seeds of the lawn plants on a mixed culture matrix for lawn planting, wherein 200mg/Kg-800mg/Kg of rare-earth cerium is contained in 1.5-2kg of mixed culture matrix soil and the mixed culture matrix is the compost of soil and municipal solid waste in a weight part ratio of 4:(1-2). According to the method provided by the invention, a certain amount of rare-earth cerium is applied, so that the photosynthetic rate of the lawn plants can be obviously increased. The application of the technology is expected to realize a high-efficiency and low-cost operating mode in large-area urban lawn planting.
Description
Technical field
The present invention relates to the rare earth cerium in urban lawn build and plant technology Application for Field, particularly relate to method and the application of adopting the rare earth cerium to improve arid mixed-matrix lawn plant photosynthetic capacity.
Background technology
The lawn is as an ecosystem, and it is a huge carbon source depots, and grassland soil can absorb deposition 0.01 ~ 0.3Gt carbon every year in the world.Therefore reduced CO in the air
2, alleviate greenhouse effect to a certain extent.Secondly, the face of land cover layer of the formed densification in lawn and grass roots layer have the effect of the good control soil erosion; The temperature difference per day on the face of land can also be reduced in the lawn, therefore can alleviate the soil avalanche that soil causes because of " frost heave " effectively.There is huge floating dust adsorption capacity on the lawn, can effectively prevent reentrainment of dust, can also reduce wind speed, has prevented dirt etc.The lawn has CO in the absorbed air
2, SO
2And NO
2Etc. virulent gas etc.The lawn also has the absorption ability of dirt, so the lawn has and purify air, and reduces the ability of polluting.Urban lawn is the product of human civilization, and it both can be beautified the environment, and quiet happy attitude that the lawn is special and bright and beautiful tone are given the sense of back to nature, gives spirit and moulds.
The city artificial lawn is drought-enduring unlike the natural meadow that kind, especially anti-thirsty poor ability.The lawn root system is more shallow, is not enough in soil, absorb enough moisture, can only depend on the manual watering.Dry season the lawn to water primary water every day, even in rainy season, at least also to water twice water in every month.In case lack of water can cause lawn growth bad.Especially in the northern China city, will consume a large amount of water every year, and a year irrigation quantity is approximately 0.6 ~ 1.0 m
3/ m
2So expensive northern China urban lawn water deficient that often makes of lawn water management; Make the quality on lawn descend, long-term insufficient water, the degeneration that the lawn will be slowly; Thereby cause the lawn to lose original value and function, this time, people's selection can only be to abandon the lawn.Therefore, in today of face of mankind water resources shortage, the allotment of water resource will be that turf establishment is gone up the ultimate challenge that people face with management with utilizing.
Rare earth element is applied to the land for growing field crops plant, increases seed vitality, promotes plant growing; Increase crop yield, a large amount of research shows that also rare earth element helps increasing the activity of antioxidase in the plant corpus, improves the anti-adversity ability of plant greatly.Show through long-term rare earth application fundamental research: rare earth element can improve chlorophyll content, the enhancing photosynthesis of plant, and the growth of promotion root system, increase root system are to nutrient absorbing.Rare earth can also promote seed germination, increases seed germination rate, promote growth of seedling.
Application about rare earth element; Existing document is coerced down the report of the influence of barley seed sprouting to acid rain relevant for cerium; Also have cerous nitrate that rice paddy seed vigor and duration of germination enzymic activity influenced document such as report; They all belong to the crops subject, but relevant rare earth element (rare earth cerium) is not seen report as yet to the research of lawn plant photosynthetic capacity.Also have document to have report " effect of rare earth in the meadow produces to be arranged " in addition, affiliated professional domain is the herding subject, and the meadow produces specialty, and forage grass that relates to or herbage and lawn are irrelevant.The soil (or location) that meadow wherein refers to and can herd, mows and develop animal husbandry, its purpose provide animal product such as meat, milk, hair for people live.Professional domain under the lawn is the gardening subject, and the ornamental horticulture specialty refers to and is used for urban afforestation and ecology service is provided for the city.There is essential difference on lawn and meadow.Lawn plant the root system vigor of seedling phase a little less than; The overground part poor growth; And the more weak characteristic of this early growth of lawn plant often makes lawn plant in the competition of growth early stage and weeds, be in a disadvantageous position, and such result can cause the turf establishment failure; In addition, this situation is under the environmental condition of drought and water shortage, and the phenomenon that lawn plant is in a disadvantageous position in the competition of growth early stage and weeds is particularly outstanding; This directly has influence on the survival rate of lawn plant; This also is that turf establishment is used greatest difficulty in practice, i.e. the problem place of turf establishment experience technology " bottleneck " restriction.The inventor was on December 05th, 2006; Applied for that " application (2006101298780) of cerous nitrate in turf establishment technology " emphasis discloses the application of cerous nitrate aspect the raising seed vitality; The inventor has further studied employing rare earth cerium again and has improved arid mixed-matrix lawn plant photosynthetic capacity method on this basis, and the application aspect photosynthetic to lawn plant under different gradient drought stress conditions.
Summary of the invention
The invention discloses a kind of method that adopts the rare earth cerium to improve arid mixed-matrix lawn plant photosynthetic capacity, it is characterized in that being undertaken by following step:
(1) the lawn plant seed is sowed on the Mixed culture matrix of turf establishment, thickness of sowing is 35.0g/m
2-45.0g/m
2, 1.5-2kg Mixed culture matrix soil contains rare earth cerium 200 mg/Kg～800 mg/Kg, and at the sowing initial stage, every basin fully waters, and the assurance seed can be sprouted the growth with the seedling initial stage smoothly;
According to coercing the degree quantitative water supply, each was weighed to two days and waters later on, to maintain in the scope of coercing after (2) three week; The wherein said degree quantitative water supply of coercing refers to: adopting weight method control normal water supply is the maxmun field capacity of 70～80% (weight), and it is 55～65% (weight) that moderate is coerced, and it is 35～50% (weight) that severe is coerced;
(3) culture condition is: in humidity is 68%～78%, carries out illumination daytime, and temperature is 22～26 ℃, and nocturnal temperature is 18～22 ℃, cultivates 40～60 days, and intensity of illumination is 800 μ mol m
-2s
-1-900 μ mol m
-2s
-1
(4) management expectancy by common lawn plant carries out in the lawn plant planting process; Treat that lawn plant grows into 40 days to 50 days, the clip blade is measured; The ratio of weight and number that wherein said Mixed culture matrix refers to is soil and the producing fertilizer from refuse in daily life of 4:1-2.Producing fertilizer from refuse in daily life wherein is meant: garbage compost is removed foreign material under 105 ℃ of conditions, dry to constant weight and use.
Method of the present invention, its middle rare earth cerium weight is 500mg/Kg.
Lawn plant seed of the present invention be English ryegrass (
Lolium perenne.L) or Festuca Arundinacea (draw
Festuca arundinacea.L).
Water of the present invention is coerced and referred to: it is 55～65% (weight) that moderate is coerced, and it is 35～50% (weight) that severe is coerced;
Rare earth cerium of the present invention refers to cerous nitrate, Ce (NO
3)
36H
2O.
Weight method is adopted in the control of degree of drought according to the invention.
The present invention further discloses and adopt rare earth cerium associating Mixed culture matrix turf plant plant in the application that improves the plant photosynthesis ability to, rare earth cerium weight wherein is 500mg/Kg.
The more detailed step of the present invention is following:
Water regime is the key factor that influences photosynthesis of plant, and the degree that wanes of soil moisture can be carried out through stomatal conductance photosynthetic influence, also can directly have influence on the photosynthetic capacity of mesophyll cell.This experimental study the influence of cerium and water utilization characteristic photosynthetic under different gradient drought stress conditions to lawn plant, from photosynthetic and of the influence of water utilization characteristic aspect reflection rare-earth element cerium to the lawn plant drought resistance.
1 materials and methods
1.1 experiment material
This experiment select for use the more common English ryegrass of northern China (
Lolium perenneL.) and Festuca Arundinacea (
Festuca arundinaceaL
.) be experiment material.Potted plant method is adopted in experiment, and basin mouth diameter is 18 cm, and height is 15cm.Lawn plant culture matrix weight is every basin 2.0Kg, 1.6Kg garden mould wherein, producing fertilizer from refuse in daily life 0.4Kg.Every basin seeding lawn grass seeds 0.9 g.Rare earth is a cerous nitrate, and molecular formula is: Ce (NO
3)
36H
2O.Producing fertilizer from refuse in daily life wherein is meant: garbage compost is removed foreign material under 105 ℃ of conditions, dry to constant weight and use.
1.2 experimental technique
Adopt the design of two factor randomized block experiments, one of factor is a drought stress, and soil moisture is made as three levels: normal water supply (T1), moderate are coerced (T2), severe is coerced (T3).The soil maxmun field capacity is 50.25%; Drought stress intensity does, contrast is that (Max Field Capacity is called for short: MFC), moderate is 55～65% MFC for 70～80% maxmun field capacity; Severe is 35～50% MFC, and weight method is adopted in the control of degree of drought.Two of factor is that the rare earth cerium concentration is made as 0,200,500,800 mgKg
-1Four levels.With 3 * 4 totally 12 processing respectively repeat 3 times.Contrast is normal water supply (T1) and 0 mgKg
-1Processing.
1.3 sample plot overview and turf establishment
Experiment is located to be carried out in the sample plot of Tianjin Normal University six Li Tai school districts, and in sample plot with bamboo pole and Plastic film greenhouse, the cultivation of whole lawn plant is tested in the greenhouse, carries out.The greenhouse is covered as the movable plastic film, and like this, fine day can be removed plastic film, so that plant can fully be accepted sunlight.The sample plot Natural situation is: July, mean temperature was that 26.6 ℃ of average relative humidities are 76%, and August, mean temperature was 25.6 ℃, and average relative humidity is 77%, and September, mean temperature was that 20.9 ℃ of average relative humidities are 68%.
The plant cultivation is carried out in this experiment since on July 26th, 2005 to September 26.At the sowing initial stage, every basin fully waters, and guarantees that seed can sprout the growth with the seedling initial stage smoothly, after three weeks according to coercing the degree quantitative water supply.Reached the various degree of coercing to August 26, each was weighed to two days and waters later on, to maintain in the scope of coercing.62d begins to measure each index: the determination of activity of proline, MDA, chlorophyll and protective enzyme.
1.4 index determining
Adopt the portable photosynthetic appearance of Li-6400 to measure stomatal conductance (Gs), intercellular CO
2Concentration (Ci), photosynthetic rate (Pn) and blade face transpiration rate indexs such as (Tr) are measured; Each is handled and respectively to survey 2 blades earlier, measures 10 data at every turn, repeats three circulations of branch and has surveyed for three times; 15 data of each replication; Minute is 9:00-10:30 in the morning, and it is fine to measure day, CO
2Concentration is 400 ± 50 μ mol CO
2Mol
-1, intensity of illumination is 900 ± 30 μ molm
-2s
-1Calculate WUEL with following formula: WUEL=photosynthetic rate/transpiration rate.Plant is cut at root, be divided into ground and underground two parts and place baking oven to dry to constant weight for 80 ℃, measure biomass.The mensuration of chlorophyll content (Su Zhengshu, 1989) is soaked with absolute ethyl alcohol and acetone 1:1 mixed solution 25 mL, with spectrophotometric determination 630 nm, the light absorption value of 654 nm.
2 results and analysis
2.1 cerium is to the influence of photosynthesis characteristics under the drought stress
(1) stomatal conductance
Pore is that plant leaf blade carries out gas exchange and the passage that carries out transpiration, and drought stress makes the stomatal conductance of perennial ryegrass blade descend (Fig. 1), and along with the increase stomatal conductance of degree of drought sharply descends, severe is coerced down 0 mgKg
-1Handle comparison according to descending 40.3%, after applying a certain amount of rare earth cerium, stomatal conductance increases to some extent, and along with the increase of the degree of coercing, rising earlier afterwards descends, and moderate is coerced air holes degree of leading and reached the highest, 500 mgKg
-1With 800 mgKg
-1Concentration effect the most obvious, degree of the leading maximum under various water conditions, moderate is coerced down and is respectively 0.163
mMolH
2Om
-2s
-1With 0.171
mMolH
2Om
-2s
-1, exceed contrast 32.5% and 39.4% respectively; Severe is coerced the serious stomatal conductance that reduces the perennial ryegrass blade, 500 mgKg
-1It is the highest to handle air holes degree of leading, and has also descended 33.9% but compare with contrast.
Under the normal water supply, along with the increase of rare earth cerium concentration, the stomatal conductance of Festuca Arundinacea increases afterwards earlier and reduces (Fig. 1), 500 mgKg
-1Cerium concentration is handled maximum, surpasses contrast 92.3%, and the Festuca Arundinacea stomatal conductance of cerium after handling all apparently higher than contrast and contrast produce notable difference (
P<0.05).Under the situation that does not apply the external source cerium, the Festuca Arundinacea stomatal conductance does not have significant change under each drought stress, and the Festuca Arundinacea stomatal conductance of cerium after handling be under same concentration, along with the increase of degree of drought reduces gradually.Remove Severe drought and coerce down 800 mgKg
-1Processing outside, the Festuca Arundinacea stomatal conductance is under each drought stress gradient, the degree of leading after handling through cerium is all apparently higher than there not being treated degree of leading.Festuca Arundinacea stomatal conductance after cerium is handled is with 500 mgKg
-1Stomatal conductance under handling is the highest, under each drought stress, all shows the highest stomatal conductance.
(2) intercellular gas concentration lwevel
Perennial ryegrass intercellular gas concentration lwevel descends along with the increase of drought stress (Fig. 2), but descend not significantly (
P>0.05).Under the different water conditions, different rare earth cerium concentrations are inconsistent to the influence of perennial ryegrass blade intercellular gas concentration lwevel, under the normal water supply condition, and 500 mgKg
-1The perennial ryegrass intercellular gas concentration lwevel of handling is minimum, is lower than contrast 11.4%; Moderate is coerced down, 800 mgKg
-1The perennial ryegrass intercellular gas concentration lwevel of handling is minimum; Severe is coerced down 200 mgKg
-1With 500 mgKg
-1Minimum; 0 mgKg
-1Perennial ryegrass intercellular gas concentration lwevel under each water conditions all is the highest.
In the processing that does not apply cerium, medium drought has increased Festuca Arundinacea intercellular gas concentration lwevel (Fig. 2); Under the normal water supply condition, cerium also increases Festuca Arundinacea intercellular gas concentration lwevel, 200 mgKg
-1Handle, concentration is maximum, is higher than contrast 49.6%.Festuca Arundinacea is coerced down in moderate, and stomatal conductance does not obviously become between each rare earth cerium concentration, do not produce significant difference (
P>0.05).Coerce down 800 mgKg in severe
-1The Festuca Arundinacea intercellular gas concentration lwevel that cerium is handled is minimum, is lower than 0 mgKg
-117.1% of processing, but each concentration does not produce notable difference between handling yet.
(3) Net Photosynthetic Rate
Under the situation that does not apply the rare earth cerium, the perennial ryegrass photosynthetic rate is along with the increase of degree of drought (Fig. 3) on a declining curve, and moderate and severe have descended 10.7% and 33.0% respectively.The perennial ryegrass photosynthetic rate obviously increases after applying the rare earth cerium, wherein with 500 mgKg
-1Rare earth cerium treatment effect is best, and perennial ryegrass blade light and speed are maximum under each water conditions, secondly are 800 mgKg
-1The rare earth cerium of concentration is handled.500 mgKg during normal water supply
-1The rare earth cerium is handled the comparison of perennial ryegrass photosynthetic rate according to having increased by 31.2%, and the medium drought comparison is according to having increased by 37.0%, and severe is coerced down and extensively closed speed also than 0 mgKg
-1Processing high by 59.7%.After applying the rare earth cerium, under the medium drought photosynthetic rate than corresponding rare earth concentration under the normal water supply handle all high, the drought stress of visible moderate has stimulated the increase of perennial ryegrass photosynthetic rate.
Cerium can obviously promote the increase (see figure 3) of Festuca Arundinacea photosynthetic rate, and under the normal water supply condition, Festuca Arundinacea is along with the increase of cerium concentration, and photosynthetic rate rises earlier and afterwards descends, at 200 mgKg
-1The photosynthetic rate increase was the most obvious when concentration was handled, and was 2 times of contrast, secondly was 500 mgKg
-1, also comparison all is higher than contrast according to the effect that 163.7%, three kind of cerium concentration processing of increase is produced, and produces evident difference with contrast.Do not applying the processing cerium of cerium; The photosynthetic rate of Festuca Arundinacea is along with first the minimizing afterwards of the increase of degree of drought increases; Possibly be to coerce down in severe, Festuca Arundinacea increases the absorption of luminous energy, the accumulation of organic substance is increased resistivity to external world through increasing photosynthetic rate.Take all factors into consideration, cerium concentration is 200 mgKg
-1With 500 mgKg
-1Two kinds of concentration treatment effects are best, and under each water conditions, the Festuca Arundinacea photosynthetic rate is all higher relatively, under medium drought, and 200 mgKg
-1With 500 mgKg
-1Photosynthetic rate after the processing is higher than 0 mgKg respectively
-1Handle 155.8% and 161.5%.Coerce down 200 mgKg in Severe drought
-1With 500 mgKg
-1Photosynthetic rate after the processing is higher than 0 mgKg respectively
-1Handle 80.0% and 33.3%.
(4) chlorophyll content
Chlorophyll is that plant carries out in the photosynthesis process important pigment that luminous energy is absorbed, transmits and transform, and the height of its content has tangible influence to photosynthesis of plants.The perennial ryegrass chlorophyll content changes sees table 1, and along with the rare earth cerium concentration increases, its content also increases chlorophyll a, 500 mgKg under the normal water supply situation
-1Shi Hanliang is the highest, exceeds contrast 36.4%.Apply in the absence of rare earth cerium, chlorophyll a content is along with the degree of coercing increases and reduces.Can obviously alleviate drought stress to the synthetic negative effect that causes of chlorophyll a after applying the rare earth cerium, the content under the rare earth cerium of same concentrations is handled does not have significant change.The content of chlorophyll b reduces along with the increase of drought stress, and applying behind the rare earth cerium content that causes chlorophyll b because of arid reduced also has tangible mitigation, and the chlorophyll b content is little under each rare earth cerium concentration, but the rare earth cerium is 800 mgKg
-1The processing of concentration is coerced down in severe and is sharply descended.The ratio of chlorophyll a/b does not have very big variation under drought stress, under the situation that does not apply the rare earth cerium, along with the increase of the degree of coercing, the ratio of chlorophyll a/b reduces afterwards earlier and increases; Under the normal water supply situation, the ratio of low chlorophyll a/b has fallen in the high concentration rare earth cerium, visible rare earth cerium to the synthetic facilitation of chlorophyll b greater than chlorophyll a, 200 mgKg
-1It is the highest to handle ratio, and the ratio that each concentration is handled chlorophyll a/b down possibly be under drought stress, to have stimulated chlorophyll a synthetic along with the increase of the degree of coercing has the trend of rising, improves the catching and transform to resist the influence of drought stress of luminous energy.
Table 1 cerium is to the influence (mgg of perennial ryegrass chlorophyll content under the drought stress
-1FW)
Cerium can obviously promote the chlorophyllous increase of Festuca Arundinacea (seeing table 2), and after cerium was handled, chlorophyll a significantly increased, the Festuca Arundinacea chlorophyll a content after three kinds of cerium concentrations are handled all with contrast produce notable difference (
P<0.05), 500 mgKg
-1Chlorophyll a content is the highest under the concentration, surpasses contrast 30%.But, 500 mgKg
-1The rare earth cerium is handled in moderate and Severe drought and is coerced chlorophyll a content suppression ratio very fast, and 200 mgKg
-1With 800 mgKg
-1Chlorophyll a content performance after two kinds of cerium concentrations are handled is relatively stable.Under the situation that does not apply the rare earth cerium; Increase along with the drought stress degree; Chlorophyll a content increases to some extent; Severe drought is coerced down comparison according to having increased by 24.%, possibly be that Festuca Arundinacea increases photosynthesis in order to resist extraneous adverse circumstance under the drought stress, increases photosynthetic product and energy in the own body.Coerce down with 200 mgKg in Severe drought
-1With 800 mgKg
-1Chlorophyll a content is higher under two kinds of cerium consumptions.Chlorophyll b content does not show evident difference under each cerium concentration processing and each drought stress.Total chlorophyll content is under normal water supply, with 500 mgKg
-1The concentration treatment effect is best, surpasses contrast 23.1%, secondly is 200 mgKg
-1With 800 mgKg
-1, all produce notable difference with contrast.And 800 mgKg
-1Total chlorophyll content under handling changes under each drought stress degree not quite, and content is more stable relatively.After cerium was handled Festuca Arundinacea, chlorophyll a reduced 500 mgKg under the normal water supply than the ratio of b along with the increase of cerium concentration increases afterwards earlier
-1Processing ratio the highest, comparison is according to high by 30.3%, and under this concentration, ratio afterwards increases along with the increase of drought stress reduces earlier, is " v " font.Chlorophyll a remains 200 mgKg than b ratio
-1With 800 mgKg
-1The most stable under two kinds of concentration, and ratio compares higher.
Table 2 cerium is to the influence (mgg of Festuca Arundinacea chlorophyll content under the drought stress
-1FW)
2.2 cerium is to the influence of perennial ryegrass water utilization characteristic under the drought stress
(1) blade face transpiration rate
Drought stress and rare earth cerium to the influence of blade face transpiration rate all compare significantly (
P<0.05), under the situation that does not apply the rare earth cerium, the blade face transpiration rate reduces along with the increase of the degree of coercing, and moderate is coerced down photosynthetic rate and is not significantly descended, but severe is coerced down comparison according to having descended 40.1% (Fig. 4).Increased perennial ryegrass blade face transpiration rate after applying the rare earth cerium, coerced down all with 500 mgKg at normal water supply and severe
-1The rising speed in perennial ryegrass blade face handled of rare earth cerium maximum; And moderate is coerced with 800 mgKg
-1It is the highest that concentration is handled perennial ryegrass blade face transpiration rate, 500 mgKg
-1Take second place, respectively than 0 mgKg
-1Under increased by 36.4% and 31.0%.
Under the situation that does not apply external source rare earth cerium, the blade face transpiration rate of Festuca Arundinacea descends earlier and afterwards rises along with the increase of degree of drought; Under the normal water supply condition, along with the increase of rare earth concentration, decline (see figure 4) after the blade face transpiration rate rises earlier, the variation tendency of its variation tendency and photosynthetic rate is consistent.Under the normal water supply, 200 mgKg
-1Transpiration rate after concentration is handled is the highest, and the increase transpiration rate along with concentration descends then; Under the medium drought condition, 500 mgKg
-1Blade face transpiration rate after the processing is the highest, secondly is 200 mgKg
-1Processing; Coerce down in Severe drought, remain 200 mgKg
-1Transpiration rate under handling is the highest, descends 800 mgKg then along with the increase of cerium concentration
-1That handles is minimum.Generally, the blade face transpiration rate descends along with the increase of drought stress.
(2) WUEL
Increase along with drought stress; First afterwards rise (table 3) that descend of perennial ryegrass WUEL; Moderate is coerced down the perennial ryegrass WUEL and is descended; Comparison is according to having descended 8.8%, and severe is coerced then and compared according to having increased by 9.6%, and possible severe is coerced has down stimulated the raising of perennial ryegrass to the utilization ratio of moisture; 500 mgKg
-1With 800 mgKg
-1The rare earth cerium concentration under also be that moderate is coerced the utilization of obvious inhibition perennial ryegrass to moisture, improved utilization ratio under the severe to moisture.Rare earth cerium consumption is with 500 mgKg
-1Concentration can significantly increase the utilization ratio of perennial ryegrass to moisture, the perennial ryegrass after this concentration is handled is all the highest under each water supply situation to the utilization ratio of moisture, during normal water supply, comparison is according to exceeding 29.3%, severe is coerced down the highest, exceeds to contrast 64.0%.
Table 3 cerium is to the influence (%) of lawn plant WUEL under the drought stress
Cerium can obviously increase the utilization ratio (see table 3) of Festuca Arundinacea to moisture, and along with the increase of cerium concentration, Festuca Arundinacea rises earlier to WUEL and afterwards descends, with 200 mgKg
-1The concentration treatment effect is best, and under this concentration, secondly the WUEL comparison is 500 mgKg according to increasing by 53.1%
-1, comparison is according to increasing by 45.0%.Arid then obviously suppress the utilization ratio of Festuca Arundinacea to moisture, along with the increase of degree of drought, WUEL rises again after descending earlier to some extent.All in all, 200 mgKg
-1It is the most obvious that Festuca Arundinacea is improved WUEL, under this concentration, each drought stress to the Festuca Arundinacea WUEL not significantly influence all keep the highest, all remain on more than 4.5%, when moderate is coerced with Severe drought respectively than 0 mgKg
-1Processing high 77.1% and 59.7%.
2.3 ground biomass
The accumulation (Fig. 5) of drought stress obvious suppression perennial ryegrass ground biomass, through two-way analysis of variance, drought stress and rare earth cerium to the influence of perennial ryegrass ground biomass all compare significantly (
P<0.05).Along with the increase of the degree of coercing, the accumulation of perennial ryegrass ground biomass significantly reduces.But after adding the rare earth cerium, significantly increase the accumulation of perennial ryegrass ground biomass, alleviate because of drought stress and work the mischief.The consumption of rare earth cerium is with 500 mgKg
-1Effect is best, applies in the absence of rare earth cerium, and moderate is coerced to coerce with severe and made ground biomass comparison according to having reduced 20.4% and 27.9% respectively, and at 500 mgKg
-1The processing ground biomass that consumption and severe are coerced is also compared according to high.200 and 800 mgKg
-1Concentration drought stress suppressed the perennial ryegrass ground biomass also have certain mitigation.
Under the situation that does not apply rare earth, Festuca Arundinacea ground biomass under moderate is coerced slightly comparison according to high, but when severe is coerced, the Festuca Arundinacea ground biomass (see figure 5) that significantly descends.Under identical water supply level, along with the increase of rare earth cerium concentration, the Festuca Arundinacea ground biomass also significantly increases.Under the normal water supply situation, the Festuca Arundinacea ground biomass is respectively 1.45 times, 1.48 times and 1.54 times of contrast all apparently higher than contrast under three kinds of rare earth cerium concentrations, all with contrast produce significant difference (
P<0.05).Coerce down in moderate and severe, with 500 mgKg
-1Biomass is the highest under the cerium concentration, surpasses 0 mgKg respectively
-1Concentration is handled 37.1% and 37.7% of following moderate and severe.
2.4 underground biomass
The drought stress obvious suppression perennial ryegrass underground biomass accumulation (Fig. 6), through two-way analysis of variance, drought stress and rare earth cerium to the influence of perennial ryegrass underground biomass all compare significantly (
P<0.05).Along with the increase of degree of drought, the perennial ryegrass underground biomass is tangible downward trend, and after applying a certain amount of rare earth cerium, downward trend is alleviated to some extent.When rare earth cerium consumption is 200 mgKg
-1The time, moderate is coerced down with normal water supply does not have difference to the influence of perennial ryegrass underground biomass, and severe is coerced then and is obviously descended, and has descended 19.4% than normal water supply.The rare earth cerium concentration is 500 mgKg
-1The time, moderate coerces to coerce with severe does not have remarkable influence to underground biomass, significantly decreases but compare all with normal water supply.800 mgKg
-1Concentration effect is the poorest, and it obviously suppresses the underground biomass accumulation.
When not applying the external source cerium, drought stress makes the Festuca Arundinacea underground biomass descend to some extent, but not with contrast produce notable difference (
P>0.05).Under the normal water supply situation, along with the increase of cerium concentration, the Festuca Arundinacea underground biomass raises earlier and afterwards reduces (see figure 6), with 500 mgKg
-1Cerium concentration is best, is 2.0 times of contrast.Under the medium drought, also be 500 mgKg
-1The cerium concentration effect is best, surpasses 0 mgKg
-1Concentration is handled 85.3% of following medium drought.Coerce down 800 mgKg in Severe drought
-1The concentration processing list reveals higher underground biomass.
2.5 influence to total biomass and root/shoot ratio
Drought stress, rare earth cerium concentration and drought stress and rare earth cerium concentration all compare significantly (Fig. 7) to the influence of perennial ryegrass biomass.Drought stress suppresses the accumulation of total biomass, applies in the absence of rare earth cerium, and moderate is coerced the biomass comparison according to having descended 15.4%, and severe is coerced down and descended 23.5%; Applying the rare earth cerium can obviously increase the accumulation of biomass, and 500 mgKg
-1The cerium consumption is the most obvious, and moderate and severe are coerced down respectively than 0 mgKg
-1Under improved 23.2% and 32.7%.Under the normal water supply situation, 200 mgKg
-1The rare earth cerium can obviously improve the perennial ryegrass root/shoot ratio, but 500 and 800 mgKg
-1Handle down, root/shoot ratio obviously descends, this explanation, and the rare earth cerium is more obvious than underground for promoting aerial growth.Moderate is coerced the increase (Fig. 7) that has then stimulated root/shoot ratio, can 500 and 800 mgKg
-1The cerium consumption do not coerce down not obviously effect in moderate and severe.
The Festuca Arundinacea total biomass is under the normal water supply situation, along with first afterwards descend (Fig. 7) that rise of the increase of cerium concentration, with 500 mgKg
-1It is best that concentration is handled, surpass contrast 70.0% and contrast produce notable difference (
P>0.05), 200 mgKg
-1With 800 mgKg
-1Two kinds of concentration results also with contrast produce notable difference (
P>0.05).Do not applying under the rare earth cerium situation, medium drought has stimulated the accumulation of Festuca Arundinacea to biomass, and Severe drought is coerced the accumulation that has suppressed biomass.500 mgKg
-1Cerium concentration is handled the biomass that makes moderate and Severe drought coerce and is all kept the highest, and comparison is according to high 65.0% and 20.0% respectively, and Severe drought is coerced down, 500 mgKg
-1Than 0 mgKg
-1The biomass of handling is high by 26.3%.The Festuca Arundinacea root/shoot ratio is under the normal water supply situation, along with first rising of the increase of cerium concentration afterwards descends 200 mgKg
-1With 500 mgKg
-1Ratio under handling is the highest, surpasses contrast 39.7% and 37.9% respectively, all produces notable difference (Fig. 7) with contrast.Under the situation that no rare earth cerium is handled, root/shoot ratio possibly be to coerce the growth that has stimulated root system down in Severe drought to come along with first decline of the increase of degree of drought afterwards rises, and after applying the rare earth cerium, root/shoot ratio all is to descend along with the increase of degree of drought.Normal water supply and moderate are coerced under two kinds of water supply situation, all with 500 mgKg
-1The root/shoot ratio that concentration is handled is the highest, and coercing in Severe drought does not then have clear regularity.
3 conclusions
The rare earth cerium has obvious facilitation to the perennial ryegrass photosynthetic rate, can alleviate the photosynthetic rate decline that arid causes, 500 mgKg
-1The concentration treatment effect best, normal water supply and moderate are coerced down comparison photograph increase by 31.2%, 30.7% respectively, when severe is coerced also than 0 mgKg
-1Processing high by 59.7%.The perennial ryegrass leaf stomatal conductance descends along with the increase of degree of drought, and the high concentration rare earth cerium then helps the perennial ryegrass stomatal conductance to be increased.Drought stress does not have appreciable impact to the intercellular gas concentration lwevel, but the intercellular gas concentration lwevel after the processing of rare earth cerium then decreases.Along with the increase of drought stress degree, perennial ryegrass afterwards rises to first decline of the utilization ratio of moisture, and severe is coerced has stimulated the increase of perennial ryegrass to WUEL on the contrary, and 500 mgKg
-1Perennial ryegrass WUEL after concentration is handled is all the highest under each drought stress gradient, and severe is coerced down comparison according to high by 64.0%.The blade face transpiration rate descends along with the increase of drought stress, and the influence that the rare earth cerium is coerced perennial ryegrass blade face transpiration rate under the gradient to different drought is also inconsistent, coerces down in moderate, and cerium concentration increases, and the blade face transpiration rate raises.The rare earth cerium can obviously increase the photosynthetic rate of Festuca Arundinacea, with 200 mgKg
-1With 500 mgKg
-1The concentration treatment effect is best, under three kinds of water supply levels, all shows higher photosynthetic rate, all with identical water supply level under 0 mgKg
-1Handle and produce significant difference.Drought stress has caused the increase of Festuca Arundinacea stomatal conductance, but not remarkable, equally at 200 mgKg
-1With 500 mgKg
-1Under two kinds of cerium concentrations, Festuca Arundinacea all has higher stomatal conductance under three kinds of water supply levels.Drought stress has reduced the utilization ratio of Festuca Arundinacea to moisture, and the rare earth cerium then can obviously increase the utilization ratio of Festuca Arundinacea to moisture, also is with 200 mgKg
-1With 500 mgKg
-1Two kinds of cerium concentrations are best, under the medium drought respectively than 0 mgKg
-1Handle high 77.1% and 75.4%.Be 200 mgKg equally
-1With 500 mgKg
-1Festuca Arundinacea blade face transpiration rate after two kinds of cerium concentrations are handled is higher.It is thus clear that the rare earth cerium can obviously increase the photosynthetic rate of Festuca Arundinacea.
Description of drawings:
Fig. 1 is the influence of cerium to lawn plant stomatal conductance under the drought stress;
Fig. 2 is that cerium is to lawn plant intercellular gas concentration lwevel under the drought stress;
Fig. 3 is that cerium influences photosynthetic rate to the lawn plant under the drought stress;
Fig. 4 cerium is to the rising influence in lawn plant drought stress blade face;
Fig. 5 is that cerium is to the influence of dry weight on the ground of lawn plant under the drought stress;
Fig. 6 variable concentrations cerium is to the influence of the underground dry weight of lawn plant under the drought stress;
Fig. 7 is the influence of variable concentrations cerium to lawn plant total biomass and root/shoot ratio under the drought stress.
Embodiment
Below in conjunction with embodiment the present invention is described; The scheme of embodiment described here; Do not limit the present invention; One of skill in the art can make improvements and change according to spirit of the present invention, and described these improvement and variation all should be regarded as within the scope of the invention, and scope of the present invention and essence are limited claim.Cerous nitrate has commercially available, and other used reagent also have commercially available.
(1) perennial ryegrass is sowed on the Mixed culture matrix of turf establishment, 2kg Mixed culture matrix soil contains rare earth cerium 300 mg/Kg, and thickness of sowing is 35.0g/m
2, at the sowing initial stage, every basin fully waters, and the assurance seed can be sprouted the growth with the seedling initial stage smoothly;
According to coercing the degree quantitative water supply, each was weighed to two days and waters later on, to maintain in the scope of coercing after (2) three week; The wherein said degree quantitative water supply of coercing refers to: adopting weight method control normal water supply is the maxmun field capacity of 70% (weight), and moderate is 65% (weight), and severe is 35% (weight);
(3) culture condition is: in humidity is 68%%, carries out illumination daytime, and temperature is 22 ℃, and nocturnal temperature is 18 ℃, cultivates 40 days, and intensity of illumination is 800 μ mol m
-2s
-1
(4) management expectancy by common lawn plant carries out in the lawn plant perennial ryegrass planting process; Treat that perennial ryegrass grows into 40 days, the clip blade is measured; The ratio of weight and number that wherein said Mixed culture matrix refers to is soil and the producing fertilizer from refuse in daily life of 4:1.Producing fertilizer from refuse in daily life refers to: garbage compost is removed foreign material under 105 ℃ of conditions, dry to constant weight.
(1) the lawn plant tall fescue seed is sowed on the Mixed culture matrix of turf establishment, 2kg Mixed culture matrix soil contains rare earth cerium 500 mg/Kg, 45.0g/m
2At the sowing initial stage, every basin fully waters, and the assurance seed can be sprouted the growth with the seedling initial stage smoothly;
According to coercing the degree quantitative water supply, each was weighed to two days and waters later on, to maintain in the scope of coercing after (2) three week; The wherein said degree quantitative water supply of coercing refers to: adopting weight method control normal water supply is the maxmun field capacity of 80% (weight), and moderate is 55% (weight), and severe is 40% (weight);
(3) culture condition is: in humidity is 68%, carries out illumination daytime, and temperature is 26 ℃, and nocturnal temperature is 22 ℃, cultivates 60 days, and intensity of illumination is 900 μ mol m
-2s
-1
(4) management expectancy by common lawn plant carries out in the lawn plant Festuca Arundinacea planting process; Treat growth of Festuca elata to 50 day, the clip blade is measured; The ratio of weight and number that wherein said Mixed culture matrix refers to is soil and the producing fertilizer from refuse in daily life of 4:1.5.Producing fertilizer from refuse in daily life refers to: garbage compost is removed foreign material under 105 ℃ of conditions, dry to constant weight.
Claims (6)
1. method that adopts the rare earth cerium to improve arid mixed-matrix lawn plant photosynthetic capacity is characterized in that being undertaken by following step:
(1) the lawn plant seed is sowed on the Mixed culture matrix of turf establishment, thickness of sowing is 35.0g/m
2-45.0g/m
2, 1.5-2kg Mixed culture matrix soil contains rare earth cerium 200 mg/Kg～800 mg/Kg, and at the sowing initial stage, every basin fully waters, and the assurance seed can be sprouted the growth with the seedling initial stage smoothly;
According to coercing the degree quantitative water supply, each was weighed to two days and waters later on, to maintain in the scope of coercing after (2) three week; The wherein said degree quantitative water supply of coercing refers to: adopting weight method control normal water supply is the maxmun field capacity of 70～80% (weight), and it is 55～65% (weight) that moderate is coerced, and it is 35～50% (weight) that severe is coerced;
(3) culture condition is: in humidity is 68%～78%, carries out illumination daytime, and temperature is 22～26 ℃, and nocturnal temperature is 18～22 ℃, cultivates 40～60 days, and intensity of illumination is 800 μ mol m
-2s
-1-900 μ mol m
-2s
-1
(4) management expectancy by common lawn plant carries out in the lawn plant planting process; Treat that lawn plant grows into 40 days to 50 days, the clip blade is measured; The ratio of weight and number that wherein said Mixed culture matrix refers to is soil and the producing fertilizer from refuse in daily life of 4:1-2.
2. the described method of claim 1, producing fertilizer from refuse in daily life wherein is meant: garbage compost is removed foreign material under 105 ℃ of conditions, dry to constant weight and use.
3. the described method of claim 1, its middle rare earth cerium weight is 500mg/Kg.
4. the application of the said method of claim 1 aspect raising lawn plant photosynthetic rate.
5. the described application of claim 4, rare earth cerium weight wherein is 500mg/Kg.
6. the described method of claim 1, lawn plant seed wherein be English ryegrass (
Lolium perenne.L) or Festuca Arundinacea (
Festuca arundinacea.L).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210081149 CN102612946B (en) | 2012-03-26 | 2012-03-26 | Method for increasing photosynthetic capacity of drought mixed matrix lawn plants by adopting rare-earth cerium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210081149 CN102612946B (en) | 2012-03-26 | 2012-03-26 | Method for increasing photosynthetic capacity of drought mixed matrix lawn plants by adopting rare-earth cerium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102612946A true CN102612946A (en) | 2012-08-01 |
| CN102612946B CN102612946B (en) | 2013-05-08 |
Family
ID=46553429
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210081149 Expired - Fee Related CN102612946B (en) | 2012-03-26 | 2012-03-26 | Method for increasing photosynthetic capacity of drought mixed matrix lawn plants by adopting rare-earth cerium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102612946B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103265345A (en) * | 2013-05-15 | 2013-08-28 | 天津师范大学 | Method for regulating drought resistance of festuca arundinacea by sludge/rare-earth mixed seed soaking solution |
| CN103270868A (en) * | 2013-05-15 | 2013-09-04 | 天津师范大学 | Method for improving salt tolerance of festuca ovina by hybrid seed soaking liquid of sludge and rare earth |
| CN104335805A (en) * | 2013-05-15 | 2015-02-11 | 天津师范大学 | Method for regulating growth of festuca arundinacea in summer-heat environment by sludge mixed seed soaking liquid |
| CN109748732A (en) * | 2019-03-14 | 2019-05-14 | 苏州科技大学 | A kind of microbial manure and preparation method thereof containing nano rare earth cerium |
| CN112616486A (en) * | 2016-04-28 | 2021-04-09 | 首尔伟傲世有限公司 | Growth and bioactive substance promoting system for medicinal crops |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1961634A (en) * | 2006-12-05 | 2007-05-16 | 天津师范大学 | Application of cerous nitrate in lawn planting technique |
-
2012
- 2012-03-26 CN CN 201210081149 patent/CN102612946B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1961634A (en) * | 2006-12-05 | 2007-05-16 | 天津师范大学 | Application of cerous nitrate in lawn planting technique |
Non-Patent Citations (3)
| Title |
|---|
| 李东林登: "高羊茅草坪草生长发育与抗逆效应研究进展", 《中国农学通报》, vol. 27, no. 19, 10 July 2011 (2011-07-10) * |
| 腾萌等: "土培草坪植物对外源铈的生态响应特征", 《稀土》, vol. 29, no. 1, 28 February 2008 (2008-02-28) * |
| 谢永荣等: "稀土元素对作物生长的影响", 《作物杂志》, no. 1, 28 February 2011 (2011-02-28) * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103265345A (en) * | 2013-05-15 | 2013-08-28 | 天津师范大学 | Method for regulating drought resistance of festuca arundinacea by sludge/rare-earth mixed seed soaking solution |
| CN103270868A (en) * | 2013-05-15 | 2013-09-04 | 天津师范大学 | Method for improving salt tolerance of festuca ovina by hybrid seed soaking liquid of sludge and rare earth |
| CN104335805A (en) * | 2013-05-15 | 2015-02-11 | 天津师范大学 | Method for regulating growth of festuca arundinacea in summer-heat environment by sludge mixed seed soaking liquid |
| CN103270868B (en) * | 2013-05-15 | 2015-04-22 | 天津师范大学 | Method for improving salt tolerance of festuca ovina by hybrid seed soaking liquid of sludge and rare earth |
| CN103265345B (en) * | 2013-05-15 | 2015-06-03 | 天津师范大学 | Method for regulating drought resistance of festuca arundinacea by sludge/rare-earth mixed seed soaking solution |
| CN112616486A (en) * | 2016-04-28 | 2021-04-09 | 首尔伟傲世有限公司 | Growth and bioactive substance promoting system for medicinal crops |
| CN112616486B (en) * | 2016-04-28 | 2022-11-22 | 首尔伟傲世有限公司 | Growth and physiological active substance promoting system of Ixeris denticulata |
| CN109748732A (en) * | 2019-03-14 | 2019-05-14 | 苏州科技大学 | A kind of microbial manure and preparation method thereof containing nano rare earth cerium |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102612946B (en) | 2013-05-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101884278B (en) | Method for improving activity of protective enzyme of festuca arundinacea under drought conditions | |
| CN101869031B (en) | Method for improving drought resistance of drought substrate tall fescue by adopting fine garbage compost | |
| CN103999671A (en) | Method for growing lantana montevidensis on wall of tea garden terrace to preserve water and soil | |
| CN102612946B (en) | Method for increasing photosynthetic capacity of drought mixed matrix lawn plants by adopting rare-earth cerium | |
| CN101869029B (en) | Method for enhancing drought resistance of festuca arundinacea by adopting domesticated plant seeds | |
| CN103694039B (en) | The light cultivation matrix of Hubei Province, a kind of river Camellia fraternal breeding and container seedling culture method thereof | |
| CN102612950B (en) | Method for planting lawn plants by combining rare earth cerium with mixed culture matrices | |
| CN102550271B (en) | Method for commercialized cultivation of container seedling of cold-resistant japanese cinnamon germplasm | |
| CN102598987B (en) | Method for using cerium to improve chlorophyll fluorescence dynamic of ryegrass in arid environments | |
| CN101147453A (en) | Artificial cultivation method for taxus chinenesis containing high content taxol | |
| CN103430843B (en) | A kind of pithecellobium clypearia seed seedling stem section quick breeding method for tissue culture | |
| CN102598986B (en) | Method for improving chlorophyll fluorescence power of festuca arundinacea under dry condition by cerium | |
| CN102612945B (en) | Method for increasing water use efficiency of drought mixed matrix lawn plants by adopting rare-earth cerium | |
| CN103299820A (en) | Culturing method for improving content of paclitaxel in taxus chinensis | |
| CN102301885A (en) | Method for planting Aegiceras corniculatum in fresh water basin | |
| CN103262860B (en) | Method for regulating growth of festuca arundinacea in hot summer environment by sludge mixed seed soaking solution | |
| CN107155697A (en) | It is a kind of to be used for the mixed culture matrix of southern roof greening | |
| CN102599025A (en) | Applications of rare earth Cerium in improving root growth rate and chlorophyll content of lawn plants | |
| CN102893802A (en) | Seedling strengthening method for rapidly cultivating cured tobacco seedlings by curing barns | |
| CN102598988A (en) | Method for improving aridity of ryegrass by using rare earth lanthanum | |
| CN102612949B (en) | Method for improving cultivating property of Festuca arundinacea turf on garbage compost substrate | |
| CN102227978B (en) | Method for adjusting malondialdehyde and proline in lolium perenne L. under water stress by adopting belt seeding | |
| CN111264342A (en) | Light matrix for raising seedlings of Quercus liaotungensis and method for artificially raising seedlings by utilizing light matrix for raising seedlings of Quercus liaotungensis | |
| CN103749202A (en) | Culture medium for container-culturing ilex cornuta | |
| CN102599037A (en) | Method for spraying cerium to leaves to improve mineral nutrition absorption for ryegrass |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130508 Termination date: 20140326 |