CN102599037A - Method for spraying cerium to leaves to improve mineral nutrition absorption for ryegrass - Google Patents
Method for spraying cerium to leaves to improve mineral nutrition absorption for ryegrass Download PDFInfo
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- CN102599037A CN102599037A CN2012100811445A CN201210081144A CN102599037A CN 102599037 A CN102599037 A CN 102599037A CN 2012100811445 A CN2012100811445 A CN 2012100811445A CN 201210081144 A CN201210081144 A CN 201210081144A CN 102599037 A CN102599037 A CN 102599037A
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Abstract
The invention discloses a method for spraying cerium to leaves to improve mineral nutrition absorption for ryegrass, which includes: covering containers with a plastic net, allowing the surface of the net to recess inward, adding Hoagland's nutrient solution into each container, allowing the concave surface of the plastic net to contact with liquid surfaces, covering the plastic net with two gauze layers to form a germination bed, and allowing seeds to germinate on the germination bed; sowing full seeds of lawn plants, adding distilled water once to supplement lost water due to vaporization, and changing the nutrient solution once a week; after the seeds germinate and grow for two weeks, and spraying or dipping different concentrations of rare-earth cerium to the leaves; dipping the plant leaves into rear-earth cerium solution for 3-5 seconds prior to taking out, wherein the concentration of the rear-earth cerium is set as 300mg/L-1-900mg/L-1; spraying the rear-earth cerium solution for three times, once two weeks, totally culturing for 56 days, and testing indexes. Results show that foliar spray of the rear-earth cerium helps the lawn plants absorb mineral elements.
Description
Technical field
The present invention relates to adopt the foliage spray cerium to improve the method that the perennial ryegrass mineral nutrition absorbs.
Background technology
The lawn is as an ecosystem, and it is a huge carbon source depots, and grassland soil can absorb deposition 0.01 ~ 0.3Gt carbon every year in the world.Therefore reduced CO in the air
2, alleviate greenhouse effect to a certain extent.Secondly, the face of land cover layer of the formed densification in lawn and grass roots layer have the effect of the good control soil erosion; The temperature difference per day on the face of land can also be reduced in the lawn, therefore can alleviate the soil avalanche that soil causes because of " frost heave " effectively.There is huge floating dust adsorption capacity on the lawn, can effectively prevent reentrainment of dust, can also reduce wind speed, has prevented dirt etc.The lawn has CO in the absorbed air
2, SO
2And NO
2Etc. virulent gas etc.The lawn also has the absorption ability of dirt, so the lawn has and purify air, and reduces the ability of polluting.Urban lawn is the product of human civilization, and it both can be beautified the environment, and quiet happy attitude that the lawn is special and bright and beautiful tone are given the sense of back to nature, gives spirit and moulds.
The city artificial lawn is drought-enduring unlike the natural meadow that kind, especially anti-thirsty poor ability.The lawn root system is more shallow, is not enough in soil, absorb enough moisture, can only depend on the manual watering.Dry season the lawn to water primary water every day, even in rainy season, at least also to water twice water in every month.In case lack of water can cause lawn growth bad.Especially in the northern China city, will consume a large amount of water every year, and a year irrigation quantity is approximately 0.6 ~ 1.0 m
3/ m
2So expensive northern China urban lawn water deficient that often makes of lawn water management; Make the quality on lawn descend, long-term insufficient water, the degeneration that the lawn will be slowly; Thereby cause the lawn to lose original value and function, this time, people's selection can only be to abandon the lawn.Therefore, in today of face of mankind water resources shortage, the allotment of water resource will be that turf establishment is gone up the ultimate challenge that people face with management with utilizing.
Rare earth element is applied to field crop, increases seed vitality, promotes plant growing; Increase crop yield, a large amount of research shows that also rare earth element helps increasing the activity of antioxidase in the plant corpus, improves the anti-adversity ability of plant greatly.Show through long-term rare earth application fundamental research: rare earth element can improve chlorophyll content, the enhancing photosynthesis of plant, and the growth of promotion root system, increase root system are to nutrient absorbing.Rare earth can also promote seed germination, increases seed germination rate, promote growth of seedling.
Application about rare earth element; Existing document is coerced down the report of the influence of barley seed sprouting to acid rain relevant for cerium; Also have cerous nitrate that rice paddy seed vigor and duration of germination enzymic activity influenced document such as report; They all belong to the crops subject, do not see report as yet but relevant foliage spray cerium improves the research of perennial ryegrass mineral nutrition absorption.Also have document to have report " effect of rare earth in the meadow produces to be arranged " in addition, affiliated professional domain is the herding subject, and the meadow produces specialty, and forage grass that relates to or herbage and lawn are irrelevant.The soil (or location) that meadow wherein refers to and can herd, mows and develop animal husbandry, its purpose provide animal product such as meat, milk, hair for people live.Professional domain under the lawn is the gardening subject, and the ornamental horticulture specialty refers to and is used for urban afforestation and ecology service is provided for the city.There is essential difference on lawn and meadow.Lawn plant the root system vigor of seedling phase a little less than; The overground part poor growth; And the more weak characteristic of this early growth of lawn plant often makes lawn plant in the competition of growth early stage and weeds, be in a disadvantageous position, and such result can cause the turf establishment failure; In addition, this situation is under the environmental condition of drought and water shortage, and the phenomenon that lawn plant is in a disadvantageous position in the competition of growth early stage and weeds is particularly outstanding; This directly has influence on the survival rate of lawn plant; This also is that turf establishment is used greatest difficulty in practice, i.e. the problem place of turf establishment experience technology " bottleneck " restriction.The inventor was on December 05th, 2006; Applied for that " application (2006101298780) of cerous nitrate in turf establishment technology " emphasis discloses the application of cerous nitrate aspect the raising seed vitality, the inventor has further studied the application that the rare earth cerium improves perennial ryegrass mineral nutrition absorption aspect again on this basis.
Summary of the invention
A kind of foliage spray cerium that adopts of the present invention improves the method that the perennial ryegrass mineral nutrition absorbs, and it is characterized in that being undertaken by following step:
(1) plastic covering net on container lets wire side cave inward, and adds Hoagland ' s nutrient solution in the container, and the plastic wire concave surface contacts with liquid level, covers two-layer gauze above the plastic wire, as germinating bed, lets seed sprout in the above;
(2) with the thickness of sowing be 35.0g/m
2-45.0g/m
2, the lawn plant seed that the sowing grain is full adds first water and replenishes the moisture lost by evaporation, and jede Woche is changed one time of nutrition liquid;
(3) seed germination and after the fortnight of growing is at foliage-spray or dip the rare earth cerium of variable concentrations; The rare earth cerium concentration is made as 300 mgL
-1-900 mgL
-1The rare earth cerium of foliage-spray variable concentrations or the amount of dipping are as the criterion so that the blade face is moistening;
(4) when changing nutrient solution, all pour out nutrient solution, fill 3-5 second in the rare earth cerium solution to the plant leaf blade immersion then, take out then, the rare earth cerium concentration is made as 300 mgL
-1-900 mgL
-1
(5) add per fortnight 1 time, totally 3 times, cultivate 56d altogether, measure each index then.
Wherein plant is at the experiment indoor cultivation, every day plant to receive the time of illumination be 7-8 h, the room temperature mean temperature is 18 ℃-20.7 ℃; Minimum temperature is 15.3 ℃, and medial humidity is 37.1% ,-39.7%; Average minimum humidity 34.5%, average light quantum density is 600 ~ 800
μMolm
-2S
-1
Preferred rare earth cerium concentration is made as 300 mgL
-1-600 mgL
-1
The more detailed preparation method of the present invention is following:
When plant absorbs moisture from soil, also from soil, absorbing various mineral elements, is to keep normal vital movement significant process on the one hand, and the opposing party can regulate the osmotic pressure in the plant corpus and improve the resistance (Wu Weihua, 2003) of plant.Rare earth element is not the necessary element of plant, but its useful element that is plant, it helps growth and development of plant, promotes the metabolism of plant.The method that experiment adopts the blade face to apply the rare earth cerium has been studied lawn plant growth and mineral element absorption and effect.
1 materials and methods
1.1 experiment material
This experiment select for use English ryegrass (
Lolium perenneL.) lawn plant.Ce elements is a cerous nitrate, and its molecular formula is: Ce (NO
3)
36H
2O.Training method is a water planting, and nutrient solution is a Hoagland ' s nutrient solution.
1.2 experimental technique
(1) the no earth culture of plant is supported
The plastic covering net ties with rubber band on the beaker of 100 ml, lets wire side cave inward; Add pancebrin in the beaker; The plastic wire concave surface contacts with liquid level, covers two-layer gauze above the plastic wire, as germinating bed; Let seed sprout in the above, the lawn plant seed of 100 full seeds of every glass of sowing.The liquid level place uses the marking pen marking, sooner or later respectively adds first water and replenishes the moisture lost by evaporation, and jede Woche is changed one time of nutrition liquid.Seed germination and after the fortnight of growing applies the rare earth cerium of variable concentrations on the blade face, adopt the method for dipping to substitute liquid level and spray; When changing nutrient solution; All pour out nutrient solution, immerse 3 seconds in the large beaker that fills rare earth cerium solution to plant leaf blade then, take out then.The rare earth cerium concentration is made as 0 mgL
-1, 300 mgL
-1, 600 mgL
-1, 900 mgL
-1Add per fortnight 1 time, totally 3 times, cultivate 56d altogether, measure each index then.
(2) environmental condition
Plant is at the experiment indoor cultivation, be placed on window by the laboratory sunny side before, every day plant to receive the time of illumination be 7-8 h.The room temperature mean temperature is 18 ℃, and average maximum is 20.7 ℃, and minimum temperature is 15.3 ℃.Medial humidity is 37.1%, and average maximal humidity is 39.7%, average minimum humidity 34.5%.Average light quantum density is 600 ~ 800
μMolm
-2S
-1
(3) index determining
Plant height adopts each cup of centimeter scale chi to survey 5 plant heights at random; The mensuration of chlorophyll content (Su Zhengshu, 1989) is soaked with absolute ethyl alcohol and acetone 1:1 mixed solution 25 mL, with spectrophotometric determination 630 nm, the light absorption value of 654 nm.The same 2.1.2.2 of chlorophyll content computational methods.Adopt the portable photosynthetic appearance measuring light curve of Li-6400.The mensuration of biomass divides the ground to put to baking oven with underground two parts plant, and 80 ℃ dry to constant weight.Adopt ICP-AES measurement lawn plant acrial part and under ground portion to macroelement and trace elements absorbed then.Macroelement: P, K, Ca, Mg; Trace element: Zn, Mn.
(4) data
Data analysis adopts EXCEL and SPSS 12.0 analysis software to handle.
Result and analysis
2.1 the blade face applies the influence of rare earth cerium to the lawn plant plant height
The blade face applies the growth (see figure 1) that the rare earth cerium helps promoting the lawn plant plant height.Along with the increase of rare earth cerium concentration, the trend that the lawn plant plant height is shown is to rise earlier and afterwards descend 300 mgL
-1Effect is best, and it is the highest that the perennial ryegrass plant height has all reached, and under this concentration, the comparison of perennial ryegrass plant height is according to exceeding 21.5%, when cerium concentration reaches 900 mgL
-1During concentration, obviously suppressed the plant height growth of perennial ryegrass.
2.2 the blade face applies the influence of rare earth cerium to the ground biomass
The blade face applies Ce elements and helps the accumulation (see figure 2) of lawn plant to biomass, and perennial ryegrass ground dry weight is along with first rising of the increase of Ce elements concentration afterwards descends 600 mgL
-1Effect after the processing is best, and ground biomass exceeds contrast 28.4% under this concentration, significant difference (
P<0.05).
2.3 the blade face applies the influence of rare earth cerium to root growth
Cerium can obviously increase the growth (seeing table 1) of lawn plant root system.When the rare earth cerium concentration is 300 mgL
-1, the rye grass roots is heavy, root long and the fibrous root number all reaches peak, surpasses contrast 50%, 24.6% and 14.3% respectively, root/shoot ratio is also compared according to high by 28.6%, but through one-way analysis of variance, difference not significantly (
P>0.05).
2.4 the blade face applies the influence of rare earth cerium to chlorophyll content
Cerium can obviously increase the chlorophyllous content of lawn plant (seeing table 2), and cerium all has obvious facilitation to perennial ryegrass chlorophyll a content and total chlorophyll content, and the increase of chlorophyll b content is then not obvious.Cerium concentration is 600 mgL
-1The time chlorophyll a content and total chlorophyll content all the highest, the chlorophyll a comparison is according to exceeding 11.3%, total chlorophyll content exceeds 11.2%, significantly increases absorption and the transformation efficiency of lawn plant to luminous energy.
Table 1 cerium is to the influence of lawn plant root system
Table 2 variable concentrations cerium is to the influence of lawn plant chlorophyll content
2.5 the light intensity response of lawn plant under the effect of cerium
(1) cerium is to the influence of lawn plant light compensation point and dark respiration speed
Cerium is handled lawn plant and is helped reducing light compensation point and the dark respiration speed (seeing table 3) of lawn plant.Perennial ryegrass is at 900 mgL
-1Light compensation point was minimum when cerium was handled, and comparison has been according to having reduced by 18.2%, and dark respiration speed is to be 600 mgL at cerium concentration
-1The time minimum, comparison has been according to having reduced by 33.6%, all with contrast the generation evident difference (
P<0.05)..
Table 3 cerium is to light compensation point and the dark respiration speed of lawn plant
(2) light intensity of photosynthetic rate response
Can find out 600 mgL from the light intensity response curve of photosynthetic rate
-1Perennial ryegrass after the rare earth cerium is handled is 800 in light intensity
μMolm
-2S
-1During the left and right sides, photosynthetic rate is higher.Perennial ryegrass is through 300 mgL
-1With 600 mgL
-1After cerium is handled, light under the high strength illumination condition, occurred and suppressed phenomenon, with 600 mgL
-1Phenomenon under handling is the most obvious, and contrast and 900 mgL
-1Light then do not occur and suppress the phenomenon (see figure 3).
(3) light intensity of stomatal conductance response
The stomatal conductance of lawn plant is 200
μMolm
-2S
-1Below sharply increase along with the increase of photosynthetic active radiation, light intensity is 200 ~ 1000
μMolm
-2S
-1Between do not have obvious variation, when light intensity surpasses 1000
μMolm
-2S
-1Along with the increase of light intensity and significantly increase.When photosynthetic active radiation surpasses 1000
μMolm
-2S
-1The time, the perennial ryegrass stomatal conductance is with 600 mgL
-1Degree of leading under the cerium concentration is minimum, 900 mgL
-1The highest (see figure 4) of handling.
(4) light intensity of intercellular gas concentration lwevel response
The intercellular gas concentration lwevel of perennial ryegrass in light intensity less than 200
μMolm
-2S
-1The time, reduce along with the increase of light intensity, maybe be in this photosynthetic active radiation scope, photosynthetic rate increases along with the increase of photosynthetic active radiation, has increased their availabilitys to the intercellular carbonic acid gas, makes the intercellular gas concentration lwevel reduce; And work as light intensity greater than 1000
μMolm
-2S
-1The time intercellular gas concentration lwevel along with the increase of light intensity and the trend of increase is arranged, possibly be the light inhibitory action when light intensity is very high, to have occurred, cause the increase (see figure 5) of the intercellular gas concentration lwevel of lawn plant.
(5) light intensity of blade face transpiration rate response
The blade face transpiration rate of lawn plant is 200
μMolm
-2S
-1Below sharply increase along with the increase of photosynthetic active radiation, light intensity is 200 ~ 1000
μMolm
-2S
-1Between rise slower, and surpass 1000 when light intensity
μMolm
-2S
-1Along with the increase of light intensity and tangible increase is arranged.When photosynthetic active radiation surpasses 1000
μMolm
-2S
-1The time, perennial ryegrass blade face transpiration rate is with 600 mgL
-1Degree of leading under the cerium concentration is minimum, 900 mgL
-1The highest (see figure 6) under the concentration.
2.6 to the mineral element absorption and effect
(1) to the influence of calcium absorption
Rare-earth element cerium helps the absorption of lawn plant to calcium, the calcium constituent that the perennial ryegrass cauline leaf is partly accumulated surpass root, (see figure 7).Perennial ryegrass obviously promotes the absorption to calcium constituent when 600 mgL-1 concentration, cauline leaf part content surpasses contrast 13.7%, and root content surpasses contrast 77%, all with contrast the generation notable difference (
P<0.05).
(2) to the potassium absorption and effect
Perennial ryegrass cauline leaf part all significantly surpasses the root (see figure 8) to the cumulant of potassium.Perennial ryegrass is at 600 mgL
-1Cerium concentration handle down, potassium content is the highest in the body, cauline leaf partly is up to 48.5 mgg
-1, the high-load of root is 40.3 mgg
-1, surpass contrast 12.8% and 26.3% respectively, all with contrast generation notable difference (
P<0.05)
(3) to the phosphorus absorption and effect
The content of phosphorus in the perennial ryegrass cauline leaf is with 600 mgL
-1Concentration is handled best, 300 mgL
-1Concentration is handled and to be taken second place, but all with contrast produce notable difference (
P<0.05), comparison is according to high 9.7% and 8.3% (see figure 9) respectively; Content in root is with 300 mgL
-1Handle preferably, comparison is according to high by 83.3%, significant difference (
P<0.05).
(4) to the magnesium absorption and effect
Magnesium is to form chlorophyllous important element, and the content of part is apparently higher than the content of under ground portion on the ground.This experiment has also proved this point, and content of magnesium is apparently higher than the heavy content (see figure 10) of root in the perennial ryegrass.Perennial ryegrass is at 600 mgL
-1Cerium concentration is handled down, and content of magnesium is the highest, and cauline leaf and root are compared respectively according to high 13.8% and 60.2%, all with contrast the generation notable difference (
P<0.05).
(5) to the manganese absorption and effect
Manganese content in the lawn plant body is along with first afterwards descend (the seeing Figure 11) that rise of the increase of cerium concentration, 300 mgL
-1With 600 mgL
-1Concentration under, content is higher relatively.Perennial ryegrass leaves and stems manganese content is 600 mgL
-1Cerium concentration is handled the highest, is 2.3 times of contrast, and root is 300 mgL
-1Cerium concentration is handled the highest, surpasses contrast 48.5%.
(6) to the zinc absorption and effect
The main root of content of zinc in the lawn plant body, cauline leaf part less (Figure 12), and along with the increase of cerium concentration, its content increases afterwards earlier and reduces.Zinc content is with 600 mgL in the rye cursive script
-1The content that cerium is handled is the highest, and content exceeds contrast 22.9% in the cauline leaf, and root exceeds 32.4%.
Brief summary
Experimental study the blade face apply the rare earth cerium to the growth of lawn plant perennial ryegrass, photosynthetic characteristic and part mineral element absorption and effect, the result shows: 300 mgL
-1The rare earth cerium can obviously promote the growth of perennial ryegrass plant height, is higher than contrast 21.5%; Best cerium concentration to promoting the perennial ryegrass ground biomass to increase is 600 mgL
-1, comparison is according to having increased by 28.4%; Ce elements does not produce significant difference with contrast to promoting the perennial ryegrass root growth not obvious; The rare earth cerium is at 600 mgL
-1Can obviously increase the chlorophyllous content of perennial ryegrass.
Record light compensation point and the dark respiration speed that the plant light curve can obtain plant by Li-6400, this measuring is the result show, the rare earth cerium has reduced the light compensation point of perennial ryegrass, for perennial ryegrass, and 600 mgL
-1Light compensation point and the dark respiration speed handled all reach minimum, and can know 600 mgL from the light intensity response curve of photosynthetic rate
-1The perennial ryegrass that concentration is handled has higher relatively maximum photosynthesis rate.
The rare earth cerium concentration is 600 mgL
-1The time can obviously promote the absorption of perennial ryegrass to macroelement, under this concentration, the calcium of perennial ryegrass acrial part and under ground portion, potassium, phosphorus, content of magnesium are the highest, macroelement then is at 300 mgL at perennial ryegrass acrial part and under ground portion content
-1Higher under the concentration, all produce notable difference with contrast.Perennial ryegrass cauline leaf part manganese content all is to be 600 mgL at cerium concentration
-1Shi Hanliang is the highest, then all is 300 mgL at root
-1Content is the highest, 600 mgL
-1Take second place.The distribution of zinc in the lawn plant body be under ground portion apparently higher than acrial part, 600 mgL
-1The rare earth cerium handle perennial ryegrass, increased its absorption to trace element zinc, in a word, the blade face applies the rare earth cerium and helps the absorption of lawn plant to mineral element.
Description of drawings:
Fig. 1 variable concentrations cerium is to the influence of lawn plant plant height;
Fig. 2 variable concentrations cerium influences the lawn plant ground biomass;
The lawn plant photosynthetic rate was to the response of light intensity under the different cerium concentrations of Fig. 3 were handled;
The lawn plant stomatal conductance was to the response of light intensity under the different cerium concentrations of Fig. 4 were handled;
The different cerium concentrations of Fig. 5 are handled lawn plant intercellular CO down
2Concentration is to the response of light intensity;
Lawn plant blade face transpiration rate was to the response of light intensity under Fig. 6 cerium was handled;
Fig. 7 variable concentrations cerium is handled lawn plant down to the response to light intensity of the contributive rate of calcium absorption;
Lawn plant was to the potassium absorption and effect under Fig. 8 variable concentrations cerium was handled;
Lawn plant was to the phosphorus absorption and effect under Fig. 9 variable concentrations cerium was handled;
Lawn plant was to the magnesium absorption and effect under Figure 10 variable concentrations cerium was handled;
Lawn plant was to the manganese absorption and effect under Figure 11 variable concentrations cerium was handled;
Lawn plant was to the zinc absorption and effect under Figure 12 variable concentrations cerium was handled.
Embodiment
Below in conjunction with embodiment the present invention is described; The scheme of embodiment described here; Do not limit the present invention; One of skill in the art can make improvements and change according to spirit of the present invention, and described these improvement and variation all should be regarded as within the scope of the invention, and scope of the present invention and essence are limited claim.Cerous nitrate, Hoagland ' s nutrient solution have commercially available, and other used reagent also have commercially available.
(1) plastic covering net on container lets wire side cave inward, and adds Hoagland ' s nutrient solution in the container, and the plastic wire concave surface contacts with liquid level, covers two-layer gauze above the plastic wire, as germinating bed, lets seed sprout in the above;
(2) with the thickness of sowing be 35.0g/m
2, the lawn plant seed (perennial ryegrass) that the sowing grain is full adds first water and replenishes the moisture lost by evaporation, and jede Woche is changed one time of nutrition liquid;
(3) seed germination and after the fortnight of growing is at foliage-spray or dip the rare earth cerium of variable concentrations; The rare earth cerium concentration is made as 300 mgL
-1The rare earth cerium of foliage-spray variable concentrations or the amount of dipping are as the criterion so that the blade face is moistening
(4) when changing nutrient solution, all pour out nutrient solution, fill 3-5 second in the rare earth cerium solution to the plant leaf blade immersion then, take out then, the rare earth cerium concentration is made as 300 mgL
-1
(5) add per fortnight 1 time, totally 3 times, cultivate 56d altogether, measure each index then.
Wherein plant is at the experiment indoor cultivation, every day plant to receive the time of illumination be 8 h, the room temperature mean temperature is 20.7 ℃, minimum temperature is 15.3 ℃, medial humidity is 37.1%, average minimum humidity 34.5%, average light quantum density is 600
μMolm
-2S
-1
(1) plastic covering net on container lets wire side cave inward, and adds Hoagland ' s nutrient solution in the container, and the plastic wire concave surface contacts with liquid level, covers two-layer gauze above the plastic wire, as germinating bed, lets seed sprout in the above;
(2) with the thickness of sowing be 45.0g/m
2, the lawn plant seed (perennial ryegrass) that the sowing grain is full adds first water and replenishes the moisture lost by evaporation, and jede Woche is changed one time of nutrition liquid;
(3) seed germination and after the fortnight of growing is at foliage-spray or dip the rare earth cerium of variable concentrations; The rare earth cerium concentration is made as 900 mgL
-1The rare earth cerium of foliage-spray variable concentrations or the amount of dipping are as the criterion so that the blade face is moistening;
(4) when changing nutrient solution, all pour out nutrient solution, fill 3-5 second in the rare earth cerium solution to the plant leaf blade immersion then, take out then, the rare earth cerium concentration is made as 900 mgL
-1
(5) add per fortnight 1 time, totally 3 times, cultivate 56d altogether, measure each index then.
Claims (4)
1. one kind is adopted the foliage spray cerium to improve the method that the perennial ryegrass mineral nutrition absorbs, and it is characterized in that being undertaken by following step:
(1) plastic covering net on container lets wire side cave inward, and adds Hoagland ' s nutrient solution in the container, and the plastic wire concave surface contacts with liquid level, covers two-layer gauze above the plastic wire, as germinating bed, lets seed sprout in the above;
(2) with the thickness of sowing be 35.0g/m
2-45.0g/m
2, the lawn plant seed that the sowing grain is full adds first water and replenishes the moisture lost by evaporation, and jede Woche is changed one time of nutrition liquid;
(3) seed germination and after the fortnight of growing is at foliage-spray or dip the rare earth cerium of variable concentrations; The rare earth cerium concentration is made as 300 mgL
-1-900 mgL
-1Its middle rare earth cerium refers to cerous nitrate, Ce (NO
3)
36H
2O;
(4) when changing nutrient solution, all pour out nutrient solution, fill 3-5 second in the rare earth cerium solution to the plant leaf blade immersion then, take out then, the rare earth cerium concentration is made as 300 mgL
-1-900 mgL
-1
(5) add per fortnight 1 time, totally 3 times, cultivate 56d altogether, measure each index then.
2. the described method of claim 1, wherein plant is at the experiment indoor cultivation, every day plant to receive the time of illumination be 7-8 h; The room temperature mean temperature is 18 ℃-20.7 ℃; Minimum temperature is 15.3 ℃, and medial humidity is 37.1% ,-39.7%; Average minimum humidity 34.5%, average light quantum density is 600 ~ 800
μMolm
-2S
-1
3. the described method of claim 1, its middle rare earth cerium refers to cerous nitrate, Ce (NO
3)
36H
2Concentration is made as 300 mgL
-1-600 mgL
-1
4. the described method of claim 1, the rare earth cerium of foliage-spray variable concentrations or the amount of dipping are as the criterion so that the blade face is moistening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100811445A CN102599037A (en) | 2012-03-26 | 2012-03-26 | Method for spraying cerium to leaves to improve mineral nutrition absorption for ryegrass |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100811445A CN102599037A (en) | 2012-03-26 | 2012-03-26 | Method for spraying cerium to leaves to improve mineral nutrition absorption for ryegrass |
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|---|---|
| CN102599037A true CN102599037A (en) | 2012-07-25 |
Family
ID=46517121
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012100811445A Pending CN102599037A (en) | 2012-03-26 | 2012-03-26 | Method for spraying cerium to leaves to improve mineral nutrition absorption for ryegrass |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109329015A (en) * | 2018-09-17 | 2019-02-15 | 南京农业大学 | A kind of method and its application using Pseudomonas fluorescence category bacterial strain Ca02 water planting rye grass |
-
2012
- 2012-03-26 CN CN2012100811445A patent/CN102599037A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 滕萌: "稀土铈在草坪中的应用以及草坪植物抗旱生理生态响应", 《天津师范大学硕士学位论文》 * |
| 滕萌等: "土培草坪植物对外源铈的生态响应特征", 《稀土》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109329015A (en) * | 2018-09-17 | 2019-02-15 | 南京农业大学 | A kind of method and its application using Pseudomonas fluorescence category bacterial strain Ca02 water planting rye grass |
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