CN102119625B - Artificial cultivation method of hemsley rockvine root - Google Patents
Artificial cultivation method of hemsley rockvine root Download PDFInfo
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Abstract
The invention discloses an artificial cultivation method of hemsley rockvine root. The artificial cultivation method comprises the following steps of: 1) selecting an annual or biennial branch which vigorously grows and is well developed and not damaged by diseases or pests, cutting the branch with the length of 4 nodes, keeping 2 leaves on the upper part of the cutting, shearing a lower cut of the cutting into a smooth diagonal plane, and soaking in 500mg/L rooting powder solution; 2) selecting field sandy soil, obliquely cutting the cutting at an angle of 35 to 45 DEG with the ground with the depth of 2 nodes of a lower part, slightly compacting the surrounding substrate and immediately watering after the cutting is completed, keeping the cutting soil humid and keeping shading and ventilation within 15 days after the cutting; and 3) transplanting the cut seedlings to fields in March and April each year, controlling cultivation density, shading by using a sunshade screen in the cultivation period, and applying a calcium fertilizer after 15 days of transplantation. By the technical scheme, the hemsley rockvine root is successfully bred and cultivated, so that a wild hemsley rockvine root resource is protected, artificial excessive collection and digging are avoided, and the requirement of clinical and scientific research medication can be met.
Description
Technical field
The present invention relates to field of plant growing technology, relate in particular to the artificial cultivation method of a kind of radix tetrastigme.
Background technology
China is the cradle of traditional Chinese medicine, and the cultivation and the production history of traditional Chinese medicine are long.Along with the arrival and human the surging of hope that go back to nature of 21 century, China's traditional Chinese herbal medicine treatment method obtains paying attention in the world again.The Chinese medicine industry that has traditional cultural feature and unique advantage to China has been brought unprecedented opportunity to develop, has also brought enormous pressure to natural resources of Chinese medicinal materials simultaneously.On the one hand, along with the development of society and the continuous deterioration of living environment, the cry of human " back to nature " is increasingly high, and the tide of " green food ", " green medicine " is risen in the whole world, and traditional medicine gains great popularity.On the other hand, along with the increase of Chinese medicine demand, enlarging day by day and backward relatively to conservation of natural environment of mankind's activity scope causes the large tracts of land vegetation to be ruined, and ecotope sharply worsens, and has aggravated the speed of wild animal and plant species extinction.The stock number of more than 100 kind of medicinal materials such as Radix Glycyrrhizae, radix tetrastigme, radix stellariae dichotomae, saline cistanche generally descends.Because the change of man's activity and ecotope, wild resource reduces day by day, and wild animals and plants herb resource commonly used is short, and the endangered species kind increases, cause frequent use 400 surplus kind of medicinal material 20% shortage is arranged every year.The needs that the requirement and the Chinese medicine of natural drug quality gone to the world in the face of countries in the world; China puts into effect laws and regulations in succession, implements the modernization of Chinese medicine, and requirement must be through the GAP of traditional Chinese medicine; Promptly pick up, to reach the purpose that increases output and stabilised quality from the source of natural drug production.
Radix tetrastigme for climbing the dried root of Calamus plant tetratigma hemsleyanum Tetrastigma hemsleyanum Diels etl Gilg in the Vitaceae precipice.Be distributed in China Zhejiang, Jiangxi, Fujian, Taiwan, Guangxi, Yunnan, provinces and regions such as Sichuan, its piece root is used as medicine, have clearing heat and detoxicating, swelling and pain relieving, antineoplastic action.Radix tetrastigme requires very harsh to growing environment, floor file growth or climb up by holding on on undershrub under the wild state generally is grown in remote, thickly forested mountains and overhanging cliff in the shade; But crown density again can not be too high; Need scattered light, it is high that soil moisture is wanted, but ventilative again; Growth cycle is longer, and natural ripening rate is extremely low.In recent years, along with going deep into of research, the antitumor action of radix tetrastigme thinks that everybody generally acknowledges, clinically and dosage among the people sharply strengthen, price of medicinal material also climbs up and up, and by one kilogram of initial dozens of yuan, rises to one kilogram of nearly 1,000 yuan.Ordering about of interests makes wild radix tetrastigme dug by mad adopting excessively, makes wild radix tetrastigme resource endangered.The radix tetrastigme wild resource is difficult to satisfy growing needs of medical treatment at all at present, even the research medication all can not ensure.
At present, the artificial cultivation of radix tetrastigme does not almost have the precedent and the experience of success.Because the radix tetrastigme ripening rate is extremely low, rely on seed growing hardly maybe.In the existing report, utilize method for tissue culture that radix tetrastigme is carried out fast breeding, but only limit to the laboratory, do not see and apply.Tissue culture is one of method of traditional Chinese medicine fast breeding at present; Its characteristics are to produce that seedling is fast, output greatly, does not receive season limit; But tissue culture also has scale of investment bigger, and technical threshold is higher, and energy resource consumption is huge; Seedling bury survival rate low with the more high shortcoming of seedling production cost, especially be inappropriate for to the medicinal herb grower of basic unit and promote.In addition, flavonoids and phenols component content are higher in the radix tetrastigme plant, very easily produce brown stain in the group training process, are difficult to set up lasting, stable high efficiency quick breeding system.Also do not see at present the radix tetrastigme field cultivation research report of standard, more do not see the report of artificial cultivation radix tetrastigme certain scale and output.In order to protect wild radix tetrastigme resource; Can give full play to simultaneously the effective use of radix tetrastigme again; Be badly in need of to form that technology is easy, the radix tetrastigme artificial breeding technology that is suitable for promoting and the method for standardized cultivation, both protected wild resource, ensured clinical application again.
Summary of the invention
In order to solve above-mentioned technical problem, the purpose of this invention is to provide a kind of radix tetrastigme artificial cultivation method.
In order to reach above-mentioned purpose, the technical scheme below the present invention has adopted:
The artificial cultivation method of a kind of radix tetrastigme comprises the steps:
1) select robust growth, physically well develop, do not have 1～2 year livings branch of damage by disease and insect, the slotting fringe of 4 joints of length keeps 2 leaves in top, the lower cut of inserting fringe is cut into a smooth scarf, with the rooting powder solution immersion of 500mg/L;
2) select the farmland sandy loam, will insert fringe and become 35 °～45 oblique cutting with ground, the degree of depth is bottom 2 joints, and cuttage finishes the compacting and watering immediately a little of matrix on every side, keeps slotting earth moistening after the cuttage within 15 days, the maintenance ventilation of shading;
3) annual 3～April cuttage seeding is transplanted into the field, the control planting density adopts the sunshade net to cover during the cultivation, transplant and use calcareous fertilisers after 15 days.
As preferably, insert fringe in the said step 1) with the rooting powder solution immersion of 500mg/L 1 hour.
As preferably, sunshade net shield coverage is 70% in the said step 3).
As preferably, planting density is seeding row spacing 20cm * 30cm in the said step 3).
As preferably, use composite fertilizer 30Kg/ mu in the said step 3), adopt the foliage spray of 100mg/L calcium nitrate solution simultaneously, amount of application 500L/ mu.
We utilize radix tetrastigme is herbaceous species, and the acrial part biomass is bigger, and saves and go up the characteristics that most adventive root are arranged, and thinks and adopts traditional cuttage to breed comparatively feasible.Simultaneously with radix tetrastigme medicinal material output, average single heavy and general flavone content as index, planting density, shield coverage, the element of investigating radix tetrastigme replenishes and factor such as transplanting time, finally confirmed the field cultivation condition.Through analytical test repeatedly, successfully set up above-mentioned artificial cultivation method, this technical method is easy and simple to handle, scale of investment is little; Promotion and implementation easily, the radix tetrastigme survival rate after the cultivation is high, and adaptability is strong; Growth cycle is short; Output is high, and bright medicine per mu yield can reach more than the 200Kg after 12 months, should be more than 50Kg after giving money as a gift.Solved the deficient difficult problem of current radix tetrastigme herb resource, can not only effectively protect wild radix tetrastigme resource, can ensure clinical again and the needs scientific research medication.
Below be our analysis and experiment to each factor in the artificial cultivation method of radix tetrastigme:
1, cutting medium is preferred:
Different plants are widely different with the conditional request of taking root to cutting medium, because substrates with different, its moisture capacity, permeability, moisture retention and gas content have nothing in common with each other.Generally require the matrix moisture capacity moderate little, also will possess good drainage and air capacity of soils, cleaning simultaneously, to contain pathogen few and be unfavorable for germ activity with variations in temperature.Visible from table 1 and table 2 result, different cutting mediums have tangible influence to the rooting rate of radix tetrastigme cuttage with the root quantity of inserting fringe of taking root, but to not obviously influence of root length.Rooting rate is arranged as from high to low: peat soil+perlite (5: 3), farmland sandy loam, farmland sandy loam+rice husk powder (1: 1), peat soil+vermiculite (5: 3), vermiculite, perlite.See that from the result to make cutting medium with peat soil+perlite (5: 3) and farmland sandy loam ideal, but consider cost factor, it is ideal to make cutting medium with the farmland sandy loam.
Table 1 different substrates is to the influence of cuttage root-taking
Table 2 different substrates is to the variance analysis of the influence of cuttage root-taking
Annotate:
* *P≤0.005
2, hormone kind and concentration is preferred
The success or failure of plant cuttage breeding except requiring certain temperature, humidity, air and nutriment condition, also need some micro-physiological activators (plant hormone).Rationally correct use hormone can improve rooting rate.Table 3 and Fig. 1 can find out, certain HORMONE TREATMENT is favourable to improving radix tetrastigme cuttage root-taking rate, along with the raising rooting rate of hormone concentration presents the back downward trend that raises earlier.When concentration is 50mg/L; The rooting rate of ABT (root-inducing powder), IAA (heteroauxin) and NAA (naa) is respectively 70.7%, 65.3% and 60.7%; When concentration is 500mg/L; Three kinds of hormone-treated radix tetrastigme rooting percents all reach peak, and the rooting rate of ABT, IAA and NAA is respectively 90.0%, 80.0% and 72.7%.Wherein, use the ABT of 500mg/L to handle slotting fringe, rooting rate is the highest.When concentration was 2000mg/L, ABT, IAA and NAA rooting rate were respectively 50.0%, 46.0% and 41.3%, have shown the poisoning effect.Variance analysis (table 4) is the result show, hormone kind and hormone concentration, and hormone kind * hormone concentration interaction all has utmost point remarkable influence to rooting rate.Synthesis result shows that the slotting fringe of ABT solution soaking that uses 500mg/L is a comparatively reasonably HORMONE TREATMENT mode of radix tetrastigme cuttage.(blank control group in the table 3 refers to and is soaked in water, and does not add any hormone)
Table 3 hormone kind and concentration are to the influence of radix tetrastigme cuttage root-taking rate
Table 4 hormone kind and concentration are to the variance analysis that influences of epicormic branch cuttage rooting rate
Annotate:
* *P≤0.005
3, different leaf areas preferred in the cutting
Blade in the cutting and terminal bud are to the important function that has of base portion adventive root.No leaf green wood cutting do not take root usually or rooting rate extremely low, experiment will be inserted fringe and is divided into five kinds and stay the leaf mode, its result of taking root sees table 5, table 6 is seen in variance analysis.Data are visible in the table, and the different fringes of inserting stay the leaf amount that the rooting rate of the slotting fringe of radix tetrastigme, root quantity and the root length of inserting fringe of taking root are had utmost point remarkable influence.Stay the rooting percent of 2 leaves the highest with the root quantity of the slotting fringe of taking root.Do not stay the rooting percent of leaf extremely low, the rooting rate that stays the entire leaf cutting fringe is 53%.The test explanation is suitably wiped out partial blade and can be promoted cuttage rooting and become to live, and then improves cuttage efficient.The blade of right quantity helps inserting the water sorption of fringe, the suction approach that has increased slotting fringe and water absorption can, make simultaneously and insert fringe and can make full use of photosynthesis and make nutrient, additional cuttage rooting is sprouted leaf to nutrient demand, sprouts leaf and becomes alive thereby be beneficial to cuttage rooting.Though and too much blade also can be made nutrient, do not form as yet owing to insert the fringe root system, and transpiration is strong, causes inserting a large amount of dehydrations of fringe and wilts dead.
The different leaf area branches of table 5 are to the influence of cuttage root-taking
The different leaf area branches of table 6 are to the variance analysis of the influence of cuttage root-taking
Annotate:
*P＜0.05,
* *P＜0.005
4, different cottage methods is preferred
Adopt straight cutting, flat inserting and three kinds of methods of oblique cutting, table 7 result can find out that flat inserting with the oblique cutting rooting rate is more or less the same; Be respectively 63% and 65%, effect obviously is superior to straight cutting, and the straight cutting rooting rate is minimum; Be merely 43.5%, the trend and the rooting rate of root quantity and root length are similar.Table 8 result can find out that different cottage methods have remarkable influence to rooting rate and root quantity, and the influence of root length is not remarkable.So select flat inserting or oblique cutting for use.Radix tetrastigme is a liane, and liane joint is in suitable environmental condition and is easy to generate aerial root, so flat the inserting of radix tetrastigme almost makes each stipes all closely contact with matrix with oblique cutting; Humidity and air capacity of soils are all better, therefore on each joint, nearly all can take root and radical more, each saves rootage duration will be early than the slotting fringe of straight cutting; Therefore average root is long also longer relatively; The flat simultaneously slotting fringe of inserting all is buried in the matrix because of whole slotting fringe almost, therefore can guarantee absorption of water, makes blade be not easy wilting; Keep strong photosynthesis, thereby promote cuttage rooting to become to live.
The different cottage methods of table 7 are to the influence of cuttage root-taking
The different cottage methods of table 8 are to the variance analysis of the influence of cuttage root-taking
Annotate:
* *P＜0.005
5, different system fringe modes is preferred
The difference preparation of inserting fringe has remarkable influence to cuttage, when fringe is inserted in preparation, requires lower cut level and smooth, in order to avoid ponding rots, influences callus and takes root.Different slotting fringe lower cut shapes have a significant effect to the radix tetrastigme cuttage root-taking, and table 9 result is visible, and it is the highest that lower cut is cut into an inclined-plane rooting rate, is 69%; But the root quantity on two inclined-planes is the highest, and the quantity of taking root that flat nick is inserted fringe is few, and table 10 The results of analysis of variance is visible; The lower cut shape has remarkable influence to rooting rate, the quantity of taking root and root length, and observes in test and find, flat nick is inserted the callus phase of fringe and the slotting fringe late 1～2d of phase than an inclined-plane of taking root; It is former because the flat nick area is too small; Be difficult for to form callus, and lower cut has increased the contact area of otch and matrix when being an inclined-plane, has promoted the formation of callus.The quantity of taking root of two slotting fringes in inclined-plane is the highest, is because the corresponding increase of otch area.Only have about 2mm but radix tetrastigme inserts the average diameter of fringe, be cut into two inclined-planes and be difficult for the processing ease splitting, perishable when cuttage, influence rooting rate.So, select to insert fringe to radix tetrastigme aborning and be cut into an inclined-plane.
The different influences of inserting fringe lower cut shape of table 9 to the radix tetrastigme cuttage root-taking
The different variance analyses of inserting fringe lower cut shape of table 10 to the influence of radix tetrastigme cuttage root-taking
Annotate:
*P＜0.05,
* *P＜0.005
What 6, all ages and classes was transplanted a cutting is preferred
Radix tetrastigme different spike branch in age has very big influence to cuttage root-taking, and its result can be found out that by table 11 1-2 gives birth to the rooting rate of inserting fringe and is up to 72%, is one of key technology of cutting survival so 1～2 year living branch selecting growth to enrich is done to transplant a cutting.Result of the test is analyzed, can be found out by table 12, fringe has utmost point remarkable influence to rooting rate age, and is not remarkable to the influence of root quantity and root length.The tender slotting fringe degree of lignification of children is minimum; But because its nutrient accumulation is lower; Its nutrient component approach exhaustion just when it also can not have enough time to grow new root system often, thus cause survival rate on the low side slightly, give birth to more than 3 years lignification insert fringe because of the meristematic capacity of the high cell of degree of lignification extremely a little less than; And on-bladed and is given birth on the slotting fringe, thereby influences its ability of taking root and quality.Therefore, should select 1-2 to give birth in the production and insert fringe to guarantee higher cuttage survival rate.
Table 11 different spike branch in age is to the influence of cuttage root-taking
Table 12 different spike branch in age is to the variance analysis of the influence of cuttage root-taking
Annotate:
*P＜005,
* *P＜0.005
What 7, different length was transplanted a cutting is preferred
Table 13 is the result show, uses the slotting fringe cuttage of 4 joints, and the mean value of rooting rate, root quantity, root length is all the highest, uses the slotting fringe cuttage of 2 joints, and the mean value of rooting rate, root quantity, root length is all minimum.The results of analysis of variance (table 14) shows that different length is inserted fringe has utmost point remarkable influence to rooting rate, and root quantity and the root length of inserting fringe of taking root had remarkable influence.
Three kinds of different stipes numbers cutting cuttage test explanations select suitable stipes number can improve rooting rate and survival rate.When analysis of experiments is thought the radix tetrastigme cuttage, transplant a cutting and be advisable, too short cutting survival is impacted with 4 stipes.Too short cutting body is little, moisture, nutrient storage deficiency; It is oversize and evaporation discharge is big to stay acrial part after the long cutting cuttage, also is prone to cause moisture, nutrient is not enough and influence rooting rate, wastes cutting bed area and cutting material simultaneously.
Table 13 different length branch is to the influence of cuttage survival rate
Table 14 different length branch is to the variance analysis of the influence of cuttage root-taking
Annotate:
* *P＜0.005
8, different cutting times is preferred
Remove June～October temperature higher, be difficult for into outside the time alive, carried out the cuttage test in every month according to the method described above; Mean temperature adopts the sunshade of sunshade net more than 25 ℃, warming with the steelframe booth when minimum temperature is lower than 0 ℃, every district organizes 50 strains; Repeat 4 times, add up the situation of taking root.Table 15 result is visible, rooting rate with the cuttage in December for the highest, May is minimum.Table 16 The results of analysis of variance, the rooting rate of different cutting times and root are long does not see notable difference, and root length difference is fairly obvious, traces it to its cause, and possibly be that 5 month temperatures are suitable, and rainwater is abundant, and it is more vigorous to grow, so the root system growing way is better.Except that the higher some months of 6-10 month temperature, the radix tetrastigme cuttage that all suits of all the other times.
The different cutting times of table 15 are to the influence of cuttage survival rate
The different cutting times of table 16 are to the variance analysis of the influence of cuttage survival rate
Annotate:
* *P＜0.005
9, different planting densities is preferred
Table 17 result is visible, the planting density seeding row spacing be controlled at 20cm * 30cm per mu yield and general flavone content the highest, single average weight is the highest with 40cm * 40cm.Table 18 The results of analysis of variance, different planting density output and single weight have than significant difference, and general flavone content difference is not obvious.Planting density is excessive, and radix tetrastigme piece root forms just less, influences the grade of medicinal material, and output is also had certain influence.The too small output that then influences of planting density has also reduced the availability in soil, so analysis-by-synthesis radix tetrastigme planting density is advisable with 20cm * 30cm.
The different planting densities of table 17 are to the influence of output and general flavone content
The different planting densities of table 18 are to the variance analysis of output and general flavone content influence
Annotate:
*P＜0.01,
* *P＜0.005
10, different shield coverages is preferred
Table 19 result is visible, and it is the highest with general flavone content that shield coverage is controlled at 70% per mu yield, and is single on average heavy the highest with 90%.Table 20 The results of analysis of variance, different shield coverage output, single weight and general flavone content difference are not obvious, but find relatively that in twos 70% covers and do not cover output notable difference is arranged.Do not cover under the situation, illumination is stronger, and the acrial part growing way is better, dark green leaf, and rattan is thicker, and internode is short, and underground part piecemeal radical amount is less, influences the output of medicinal material.During shield coverage 90%, illumination is not enough, and acrial part is elongated, the blade attenuation, and color is a light green color, and the rattan internode is longer, and diameter is thinner, and underground part piecemeal root is less, and the average weight reduction, so analysis-by-synthesis radix tetrastigme cultivation shield coverage is advisable with 70%.
The different shield coverages of table 19 are to the influence of output and general flavone content
The different shield coverages of table 20 are to the variance analysis of output and general flavone content influence
11, microelement-supplementing is preferred
Table 21 result is visible, replenishes Fe, Zn, Ca and can improve the output of radix tetrastigme, and is wherein the highest to mend Ca group output, and average single heavy and general flavone content also are that to mend the Ca group the highest.Table 22 The results of analysis of variance is visible, and replenishing different trace elements has considerable influence to radix tetrastigme output.The radix tetrastigme effect that vegetative period, spray had obvious raising output with Ca fertilizer on the blade face.
Table 21 replenishes the influence of different trace elements to output and general flavone content
Table 22 replenishes the variance analysis of different trace elements to output and general flavone content influence
Annotate:
*P＜0.05
12, transplanting time is preferred
Table 23 is visible, the radix tetrastigme output that March, April transplant and single heavy higher, and general flavone content is also higher; The radix tetrastigme output that June, December and January transplant is lower; Table 24 The results of analysis of variance, different transplanting time output have notable difference, and single heavy and general flavone content are not seen notable difference.Analysis-by-synthesis thinks that transplant March-April, and output and the general flavone content of radix tetrastigme are higher.The 5-6 month transplants, and owing to temperature raises, rainwater is more, causes rotting of transplanted seedling root easily, because temperature reduces, also influences the survival rate of transplanting December-February next year, so cause output to descend.
The different cutting times of table 23 are to the influence of cuttage survival rate
The different cutting times of table 24 are to the variance analysis of the influence of cuttage survival rate
Annotate:
* *P＜0.005
The present invention has following beneficial effect owing to adopted above technical scheme: 1, this method adopts the farmland sandy loam as cutting medium, and the cuttage process is carried out in the booth of field, and adaptability is strong, and equipment is simple, implements easily.2, take root behind the radix tetrastigme seedling replanting of survival, survival rate is high, and growth cycle is short, and output is high.3, this technology easy and simple to handle, be suitable for promoting, form industrialization easily.4, successfully breed and cultivate radix tetrastigme, both protected wild radix tetrastigme resource, avoided artificial mad adopting excessively to dig, can ensure clinical again and the needs scientific research medication.
Description of drawings
Fig. 1 is different hormone kinds and the hormone concentration sketch map that influences to rooting rate.
Embodiment
Do a detailed explanation below in conjunction with the accompanying drawing specific embodiments of the invention.
Embodiment 1:
The artificial cultivation method of a kind of radix tetrastigme comprises the steps:
1) select robust growth, physically well develop, do not have 1～2 year livings branch of damage by disease and insect, the slotting fringe of 4 joints of length keeps 2 leaves in top, the lower cut of slotting fringe is cut into a smooth scarf, the ABT of 500mg/L (root-inducing powder) solution soaking 1h.
2) select the farmland sandy loam, will insert fringe and become 35 °～45 oblique cutting with ground, the degree of depth is bottom 2 joints; Cuttage finishes the compacting and watering immediately a little of matrix on every side; Satisfy and insert the demand of fringe to moisture, and slotting fringe is fully contacted with matrix, it is moistening to keep inserting earth after the cuttage within the 15d; Keep shading ventilation, the cuttage rooting survival rate reaches more than 90% behind the 30d.
3) the annual 3-4 month transplants cuttage seeding into the field, and planting density is advisable with seeding row spacing 20cm * 30cm, adopts the sunshade net to cover during the cultivation; Shield coverage is advisable with 70%; Use composite fertilizer 30Kg/ mu after transplanting 15d, adopt the foliage spray of 100mg/L calcium nitrate solution simultaneously, amount of application 500L/ mu.Per mu yield can reach more than the 200Kg after 12 months.
Claims (4)
1. the artificial cultivation method of a radix tetrastigme is characterized in that, comprises the steps:
1) select robust growth, physically well develop, do not have 1～2 year livings branch of damage by disease and insect, the slotting fringe of 4 joints of length keeps 2 leaves in top, the lower cut of inserting fringe is cut into a smooth scarf, with the rooting powder solution immersion of 500mg/L;
2) select the farmland sandy loam, will insert fringe and become 35 °～45 oblique cutting with ground, the degree of depth is bottom 2 joints, and cuttage finishes the compacting and watering immediately a little of matrix on every side, keeps slotting earth moistening after the cuttage within 15 days, the maintenance ventilation of shading;
3) annual 3～April cuttage seeding is transplanted into the field, the control planting density adopts the sunshade net to cover during the cultivation, and shield coverage is 70%, transplants and uses calcareous fertilisers after 15 days.
2. the artificial cultivation method of a kind of radix tetrastigme according to claim 1 is characterized in that, slotting fringe soaked 1 hour with the rooting powder solution of 500mg/L in the said step 1).
3. the artificial cultivation method of a kind of radix tetrastigme according to claim 1 is characterized in that, planting density is seeding row spacing 20cm * 30cm in the said step 3).
4. the artificial cultivation method of a kind of radix tetrastigme according to claim 1 is characterized in that, uses composite fertilizer 30Kg/ mu in the said step 3), adopts the foliage spray of 100mg/L calcium nitrate solution simultaneously, amount of application 500L/ mu.
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