CN102119625A - Artificial cultivation method of hemsley rockvine root - Google Patents

Artificial cultivation method of hemsley rockvine root Download PDF

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CN102119625A
CN102119625A CN 201010606655 CN201010606655A CN102119625A CN 102119625 A CN102119625 A CN 102119625A CN 201010606655 CN201010606655 CN 201010606655 CN 201010606655 A CN201010606655 A CN 201010606655A CN 102119625 A CN102119625 A CN 102119625A
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cutting
radix tetrastigme
root
cuttage
plugged ear
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CN102119625B (en
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浦锦宝
郑军献
梁卫青
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浙江省中医药研究院
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Abstract

The invention discloses an artificial cultivation method of hemsley rockvine root. The artificial cultivation method comprises the following steps of: 1) selecting an annual or biennial branch which vigorously grows and is well developed and not damaged by diseases or pests, cutting the branch with the length of 4 nodes, keeping 2 leaves on the upper part of the cutting, shearing a lower cut of the cutting into a smooth diagonal plane, and soaking in 500mg/L rooting powder solution; 2) selecting field sandy soil, obliquely cutting the cutting at an angle of 35 to 45 DEG with the ground with the depth of 2 nodes of a lower part, slightly compacting the surrounding substrate and immediately watering after the cutting is completed, keeping the cutting soil humid and keeping shading and ventilation within 15 days after the cutting; and 3) transplanting the cut seedlings to fields in March and April each year, controlling cultivation density, shading by using a sunshade screen in the cultivation period, and applying a calcium fertilizer after 15 days of transplantation. By the technical scheme, the hemsley rockvine root is successfully bred and cultivated, so that a wild hemsley rockvine root resource is protected, artificial excessive collection and digging are avoided, and the requirement of clinical and scientific research medication can be met.

Description

The artificial cultivation method of a kind of radix tetrastigme
Technical field
The present invention relates to field of plant growing technology, relate in particular to the artificial cultivation method of a kind of radix tetrastigme.
Background technology
China is the cradle of traditional Chinese medicine, and the cultivation and the production history of traditional Chinese medicine are long.Along with arrival and human the surging of hope that go back to nature of 21 century, the traditional Chinese herbal medicine treatment method of China obtains paying attention in the world again.The Chinese medicine industry that has traditional cultural feature and unique advantage to China has been brought unprecedented opportunity to develop, has also brought enormous pressure to natural resources of Chinese medicinal materials simultaneously.On the one hand, along with the development of society and the continuous deterioration of living environment, the cry of human " back to nature " is more and more higher, and the tide of " green food ", " green medicine " is risen in the whole world, and traditional medicine gains great popularity.On the other hand, along with the increase of Chinese medicine demand, the mankind's activity scope enlarge relative backward with to conservation of natural environment day by day, cause the large tracts of land vegetation to be ruined, ecotope sharply worsens, and has aggravated the speed of wild animal and plant species extinction.The stock number of more than 100 kind of medicinal materials such as Radix Glycyrrhizae, radix tetrastigme, radix stellariae dichotomae, saline cistanche generally descends.Because the change of man's activity and ecotope, wild resource reduces day by day, wild animals and plants herb resource shortage commonly used, the endangered species kind increases, cause frequent use 400 surplus kind of medicinal material 20% shortage is arranged every year.The needs that the requirement and the Chinese medicine of natural drug quality gone to the world in the face of countries in the world, China puts into effect laws and regulations in succession, implements the modernization of Chinese medicine, and requirement must be by the standardized planting of traditional Chinese medicine, promptly pick up, to reach the purpose that increases output and stabilised quality from the source of natural drug production.
Radix tetrastigme for climbing the dried root of Calamus plant tetratigma hemsleyanum Tetrast igma hemsleyanumDiels et1 Gilg in the Vitaceae precipice.Be distributed in China Zhejiang, Jiangxi, Fujian, Taiwan, Guangxi, Yunnan, provinces and regions such as Sichuan, its piece root is used as medicine, have clearing heat and detoxicating, swelling and pain relieving, antineoplastic action.Radix tetrastigme requires very harsh to growing environment, floor file growth or climb up by holding on on undershrub under the wild state, generally be grown in remote, thickly forested mountains and overhanging cliff in the shade, but crown density again can not be too high, need scattered light, it is high that soil moisture is wanted, but ventilative again, growth cycle is longer, and natural ripening rate is extremely low.In recent years, along with going deep into of research, the antitumor action of radix tetrastigme thinks that everybody generally acknowledges, clinically and dosage among the people sharply strengthen, price of medicinal material also climbs up and up, and by one kilogram of initial dozens of yuan, rises to one kilogram of nearly 1,000 yuan.Ordering about of interests makes wild radix tetrastigme be dug by mad adopting excessively, makes wild radix tetrastigme resource endangered.The radix tetrastigme wild resource is difficult to satisfy growing needs of medical treatment at all at present, even the research medication all can not ensure.
At present, the artificial cultivation of radix tetrastigme does not almost have the precedent and the experience of success.Because the radix tetrastigme ripening rate is extremely low, rely on seed growing hardly may.In the existing report, utilize method for tissue culture that radix tetrastigme is carried out fast breeding, but only limit to the laboratory, do not see and apply.Tissue culture is one of method of traditional Chinese medicine fast breeding at present, its characteristics are to produce that seedling is fast, output greatly, be not subject to seasonal restrictions, but tissue culture also has scale of investment bigger, technical threshold is higher, energy resource consumption is huge, the seedling low and more high shortcoming of seedling production cost of survival rate of burying especially is unsuitable for promoting to the medicinal herb grower of basic unit.In addition, flavonoids and phenols component content are higher in the radix tetrastigme plant, very easily produce brown stain in the group training process, are difficult to set up lasting, stable high efficiency quick breeding system.Yet there are no the radix tetrastigme field cultivation research report of standard, more do not see the report of artificial cultivation radix tetrastigme certain scale and output.In order to protect wild radix tetrastigme resource; can give full play to simultaneously the effective use of radix tetrastigme again; be badly in need of to form that technology is easy, the radix tetrastigme artificial breeding technology that is suitable for promoting and the method for standardized cultivation, both protected wild resource, ensured clinical application again.
Summary of the invention
In order to solve above-mentioned technical problem, the purpose of this invention is to provide a kind of radix tetrastigme artificial cultivation method.
To achieve the above object, the present invention has adopted following technical scheme:
The artificial cultivation method of a kind of radix tetrastigme comprises the steps:
1) select robust growth, physically well develop, do not have 1~2 year livings branch of damage by disease and insect, the plugged ear of 4 joints of length keeps 2 leaves in top, the lower cut of plugged ear is cut into a smooth scarf, with the rooting powder solution immersion of 500mg/L;
2) select the farmland sandy loam, plugged ear is become 35 °~45 oblique cutting with ground, the degree of depth is bottom 2 joints, and cuttage finishes the compacting and watering immediately a little of matrix on every side, keeps slotting earth moistening after the cuttage within 15 days, the maintenance ventilation of shading;
3) annual 3~April cuttage seeding is transplanted into the field, the control planting density adopts the sunshade net to cover during the cultivation, transplant and use calcareous fertilisers after 15 days.
As preferably, plugged ear was soaked 1 hour with the rooting powder solution of 500mg/L in the described step 1).
As preferably, described step 2) sunshade net shield coverage is 70% in.
As preferably, planting density is seeding row spacing 20cm * 30cm in the described step 3).
As preferably, use composite fertilizer 30Kg/ mu in the described step 3), adopt the foliage spray of 100mg/L calcium nitrate solution simultaneously, amount of application 500L/ mu.
We utilize radix tetrastigme is herbaceous species, and the acrial part biomass is bigger, and saves and go up the characteristics that most adventive root are arranged, and thinks and adopts traditional cuttage to breed comparatively feasible.Simultaneously with radix tetrastigme medicinal material output, average single heavy and general flavone content as index, planting density, shield coverage, the element of investigating radix tetrastigme replenishes and factor such as transplanting time, finally determined the field cultivation condition.By analytical test repeatedly, successfully set up above-mentioned artificial cultivation method, this technical method is easy and simple to handle, scale of investment is little, promotion and implementation easily, the radix tetrastigme survival rate height after the cultivation, adaptability is strong, growth cycle is short, the output height, bright medicine per mu yield can reach more than the 200Kg after 12 months, should be more than 50Kg after giving money as a gift.Solved the difficult problem of current radix tetrastigme herb resource scarcity, can not only effectively protect wild radix tetrastigme resource, can ensure clinical again and the needs scientific research medication.
Below be our analysis and experiment to each factor in the artificial cultivation method of radix tetrastigme:
1, cutting medium is preferred:
Different plants are widely different to the cutting medium and the conditional request of taking root, because different matrix, its moisture capacity, permeability, moisture retention and gas content have nothing in common with each other.Generally require the moderate and variations in temperature of matrix moisture capacity little, also will possess good drainage and air capacity of soils, cleaning simultaneously, to contain pathogen few and be unfavorable for the germ activity.From table 1 and table 2 result as seen, different cutting mediums have tangible influence to the rooting rate of radix tetrastigme cuttage and the root quantity of the plugged ear of taking root, but to not obviously influence of root length.Rooting rate is arranged as from high to low: peat soil+perlite (5: 3), farmland sandy loam, farmland sandy loam+rice husk powder (1: 1), peat soil+vermiculite (5: 3), vermiculite, perlite.See that from the result to make cutting medium with peat soil+perlite (5: 3) and farmland sandy loam ideal, but consider cost factor, it is ideal to make cutting medium with the farmland sandy loam.
Table 1 different substrates is to the influence of cuttage root-taking
Table 2 different substrates is to the variance analysis of the influence of cuttage root-taking
Annotate: * *P≤0.005
2, hormone kind and concentration is preferred
The success or failure of plant cuttage breeding except requiring certain temperature, humidity, air and nutriment condition, also need some micro-physiological activators (plant hormone).Rationally correct use hormone can improve rooting rate.Table 3 and Fig. 1, as can be seen, certain HORMONE TREATMENT is favourable to improving radix tetrastigme cuttage root-taking rate, along with the raising rooting rate of hormone concentration presents the back downward trend that raises earlier.When concentration is 50mg/L, the rooting rate of ABT (root-inducing powder), IAA (heteroauxin) and NAA (naa) is respectively 70.7%, 65.3% and 60.7%, when concentration is 500mg/L, three kinds of hormone-treated radix tetrastigme rooting percents all reach peak, and the rooting rate of ABT, IAA and NAA is respectively 90.0%, 80.0% and 72.7%.Wherein, use the ABT of 500mg/L to handle plugged ear, rooting rate is the highest.When concentration was 2000mg/L, ABT, IAA and NAA rooting rate were respectively 50.0%, 46.0% and 41.3%, have shown the poisoning effect.Variance analysis (table 4) is the result show, hormone kind and hormone concentration, and hormone kind * hormone concentration interaction all has utmost point remarkable influence to rooting rate.Synthesis result shows that the ABT solution soaking plugged ear of using 500mg/L is a comparatively reasonably HORMONE TREATMENT mode of radix tetrastigme cuttage.(the blank group in the table 3 refers to and is soaked in water, and does not add any hormone)
Table 3 hormone kind and concentration are to the influence of radix tetrastigme cuttage root-taking rate
Table 4 hormone kind and concentration are to the variance analysis that influences of epicormic branch cuttage rooting rate
Annotate: * *P≤0.005
3, different leaf areas preferred in the cutting
Blade in the cutting and terminal bud are to the important function that has of base portion adventive root.No leaf green wood cutting do not take root usually or rooting rate extremely low, experiment is divided into five kinds with plugged ear and stays the leaf mode, it is taken root and the results are shown in Table 5, variance analysis sees Table 6.In the table data as seen, different plugged ears stay the leaf amount that the root quantity and the root length of the rooting rate of radix tetrastigme plugged ear, the plugged ear of taking root are had utmost point remarkable influence.Stay the root quantity of the rooting percent of 2 leaves and the plugged ear of taking root the highest.Do not stay the rooting percent of leaf extremely low, the rooting rate that stays the entire leaf cutting fringe is 53%.The test explanation is suitably wiped out partial blade and can be promoted cuttage rooting and survive, and then improves cuttage efficient.The blade of right quantity helps the water sorption of plugged ear, increased the suction approach of plugged ear and water absorption can, make plugged ear can make full use of photosynthesis simultaneously and make nutrient, additional cuttage rooting is sprouted leaf to nutrient demand, sprouts leaf and survives thereby be beneficial to cuttage rooting.Though and too much blade also can be made nutrient, because the formation as yet of plugged ear root system, and transpiration is strong, it is dead to cause a large amount of dehydrations of plugged ear to be wilted.
The different leaf area branches of table 5 are to the influence of cuttage root-taking
The different leaf area branches of table 6 are to the variance analysis of the influence of cuttage root-taking
Annotate: *P<0.05, * *P<0.005
4, different cottage methods is preferred
Employing straight cutting, flat inserting and three kinds of methods of oblique cutting, table 7 result puts down to insert with the oblique cutting rooting rate being more or less the same as can be seen, be respectively 63% and 65%, effect obviously is better than straight cutting, straight cutting rooting rate minimum, only be 43.5%, the trend and the rooting rate of root quantity and root length are similar.Table 8 result as can be seen, different cottage methods have remarkable influence to rooting rate and root quantity, the influence of root length is not remarkable.So select flat inserting or oblique cutting for use.Radix tetrastigme is a liane, and liane joint is in suitable environmental condition and is easy to generate aerial root, therefore flat the inserting of radix tetrastigme almost makes each stipes all closely contact with matrix with oblique cutting, humidity and air capacity of soils are all better, therefore on each joint, nearly all can take root and radical more, each saves rootage duration will be early than the plugged ear of straight cutting, therefore average root is long also longer relatively, flat simultaneously plugged ear of inserting all is buried in the matrix because of whole plugged ear almost, therefore can guarantee absorption to moisture, make blade be not easy to wilt, keep strong photosynthesis, thereby promote that cuttage rooting survives.
The different cottage methods of table 7 are to the influence of cuttage root-taking
The different cottage methods of table 8 are to the variance analysis of the influence of cuttage root-taking
Annotate: * *P<0.005
5, different system fringe modes is preferred
The difference preparation of plugged ear has remarkable influence to cuttage, when the preparation plugged ear, requires lower cut level and smooth, in order to avoid ponding rots, influences callus and takes root.Different plugged ear lower cut shapes have a significant effect to the radix tetrastigme cuttage root-taking, table 9 result as seen, it is the highest that lower cut is cut into an inclined-plane rooting rate, is 69%; But the root quantity on two inclined-planes is the highest, the quantity of taking root of flat nick plugged ear is few, table 10 The results of analysis of variance as seen, the lower cut shape has remarkable influence to rooting rate, the quantity of taking root and root length, and observe to find in test, callus phase of flat nick plugged ear and take root the phase than the late 1~2d of the plugged ear on an inclined-plane, it is former because the flat nick area is too small, be difficult for to form callus, and lower cut has increased the contact area of otch and matrix when being an inclined-plane, has promoted the formation of callus.The quantity of taking root of two inclined-plane plugged ears is the highest, is because the corresponding increase of otch area.But the average diameter of radix tetrastigme plugged ear only has about 2mm, is cut into two inclined-planes and is difficult for the processing ease splitting, and is perishable when cuttage, influences rooting rate.So, select aborning the radix tetrastigme plugged ear is cut into an inclined-plane.
The different plugged ear lower cut of table 9 shape is to the influence of radix tetrastigme cuttage root-taking
The different plugged ear lower cut of table 10 shape is to the variance analysis of radix tetrastigme cuttage root-taking influence
Annotate: *P<0.05, * *P<0.005
What 6, all ages and classes was transplanted a cutting is preferred
The different fringe branches in age of radix tetrastigme have very big influence to cuttage root-taking, its result as can be seen from Table 11, the rooting rate that 1-2 gives birth to plugged ear is up to 72%, is one of key technology of cutting survival so 1~2 year livings branch selecting growth to enrich is done cutting.Result of the test is analyzed, and as can be seen from Table 12, fringe has utmost point remarkable influence to rooting rate age, and is not remarkable to the influence of root quantity and root length.The tender plugged ear degree of lignification of children is minimum, but because its nutrient accumulation is lower, its nutrient component approach exhaustion just when it also can not have enough time to grow new root system often, thereby cause survival rate on the low side slightly, give birth to more than 3 years the lignification plugged ear because of the meristematic capacity of the high cell of degree of lignification extremely a little less than, and on-bladed and is given birth on the plugged ear, thereby influences its ability of taking root and quality.Therefore, should select 1-2 to give birth to plugged ear in the production to guarantee higher cuttage survival rate.
The different fringe of table 11 branch in age is to the influence of cuttage root-taking
The different fringe of table 12 branch in age is to the variance analysis of the influence of cuttage root-taking
Annotate: *P<005, * *P<0.005
What 7, different length was transplanted a cutting is preferred
Table 13 is the result show, uses the plugged ear cuttage of 4 joints, and the mean value of rooting rate, root quantity, root length is all the highest, uses the plugged ear cuttage of 2 joints, and the mean value of rooting rate, root quantity, root length is all minimum.The results of analysis of variance (table 14) shows that the different length plugged ear has utmost point remarkable influence to rooting rate, and the root quantity and the root length of the plugged ear of taking root had remarkable influence.
Three kinds of different stipes numbers cutting cuttage test explanations select suitable stipes number can improve rooting rate and survival rate.When analysis of experiments is thought the radix tetrastigme cuttage, transplant a cutting and be advisable, too short cutting survival is impacted with 4 stipes.Too short cutting body is little, moisture, nutrient storage deficiency; It is oversize and evaporation discharge is big to stay acrial part after the long cutting cuttage, also easily causes moisture, nutrient is not enough and influence rooting rate, wastes cutting bed area and cutting material simultaneously.
Table 13 different length branch is to the influence of cuttage survival rate
Table 14 different length branch is to the variance analysis of the influence of cuttage root-taking
Annotate: * *P<0.005
8, different cutting times is preferred
Remove June~October temperature higher, outside the time that is difficult for surviving, carried out the cuttage test in every month according to the method described above, mean temperature adopts the sunshade of sunshade net more than 25 ℃, warming with the steelframe booth when minimum temperature is lower than 0 ℃, every district organizes 50 strains, repeat 4 times, add up the situation of taking root.Table 15 result as seen, rooting rate with the cuttage in December for the highest, May is minimum.Table 16 The results of analysis of variance, the rooting rate of different cutting times and root are long does not see notable difference, and root length difference is fairly obvious, traces it to its cause, and may be that 5 month temperatures are suitable, and rainwater is abundant, and it is more vigorous to grow, so the root system growing way is better.Except that the higher some months of 6-10 month temperature, the radix tetrastigme cuttage that all suits of all the other times.
The different cutting times of table 15 are to the influence of cuttage survival rate
The different cutting times of table 16 are to the variance analysis of the influence of cuttage survival rate
Annotate: * *P<0.005
9, different planting densities is preferred
Table 17 result as seen, the planting density seeding row spacing be controlled at 20cm * 30cm per mu yield and general flavone content the highest, single average weight is the highest with 40cm * 40cm.Table 18 The results of analysis of variance, different planting density output and single weight have than significant difference, and general flavone content difference is not obvious.Planting density is excessive, and radix tetrastigme piece root forms just less, influences the grade of medicinal material, and output is also had certain influence.The too small output that then influences of planting density has also reduced the availability in soil, so analysis-by-synthesis radix tetrastigme planting density is advisable with 20cm * 30cm.
The different planting densities of table 17 are to the influence of output and general flavone content
The different planting densities of table 18 are to the variance analysis of output and general flavone content influence
Annotate: *P<0.01, * *P<0.005
10, different shield coverages is preferred
Table 19 result as seen, shield coverage be controlled at 70% per mu yield and general flavone content the highest, single average heavy the highest with 90%.Table 20 The results of analysis of variance, different shield coverage output, single weight and general flavone content difference are not obvious, but find relatively that in twos 70% covers and do not cover output notable difference is arranged.Do not cover under the situation, illumination is stronger, and the acrial part growing way is better, dark green leaf, and rattan is thicker, and internode is short, and underground part piecemeal radical amount is less, influences the output of medicinal material.During shield coverage 90%, illumination is not enough, and acrial part is elongated, the blade attenuation, and color is a light green color, and the rattan internode is longer, and diameter is thinner, and underground part piecemeal root is less, and the average weight reduction, so analysis-by-synthesis radix tetrastigme cultivation shield coverage is advisable with 70%.
The different shield coverages of table 19 are to the influence of output and general flavone content
The different shield coverages of table 20 are to the variance analysis of output and general flavone content influence
11, microelement-supplementing is preferred
As seen table 21 result replenishes Fe, Zn, Ca can improve the output of radix tetrastigme, and is wherein the highest to mend Ca group output, and average single heavy and general flavone content also is that to mend the Ca group the highest.Table 22 The results of analysis of variance as seen, replenishing different trace elements has considerable influence to radix tetrastigme output.The effect that radix tetrastigme vegetative period has obvious raising output in the blade face spray with Ca fertilizer.
Table 21 replenishes the influence of different trace elements to output and general flavone content
Table 22 replenishes the variance analysis of different trace elements to output and general flavone content influence
Annotate: *P<0.05
12, transplanting time is preferred
Table 23 as seen, the radix tetrastigme output that March, April transplant and single heavy higher, general flavone content is also higher, the radix tetrastigme output that June, December and January transplant is lower, table 24 The results of analysis of variance, different transplanting time output have notable difference, and single heavy and general flavone content is not seen notable difference.Analysis-by-synthesis thinks that transplant March-April, and output and the general flavone content of radix tetrastigme are higher.The 5-6 month transplants, and owing to temperature raises, rainwater is more, causes rotting of transplanted seedling root easily, because temperature reduces, also influences the survival rate of transplanting December-February next year, so cause output to descend.
The different cutting times of table 23 are to the influence of cuttage survival rate
The different cutting times of table 24 are to the variance analysis of the influence of cuttage survival rate
Annotate: * *P<0.005
The present invention has following beneficial effect owing to adopted above technical scheme: 1, this method adopts the farmland sandy loam as cutting medium, and the cuttage process is carried out in the booth of field, and adaptability is strong, and equipment is simple, implements easily.2, take root behind the radix tetrastigme seedling replanting of survival, the survival rate height, growth cycle is short, the output height.3, this technology easy and simple to handle, be suitable for promoting, form industrialization easily.4, successfully breed and cultivate radix tetrastigme, both protected wild radix tetrastigme resource, avoided artificial mad adopting excessively to dig, can ensure clinical again and the needs scientific research medication.
Description of drawings
Fig. 1 is different hormone kinds and the hormone concentration schematic diagram that influences to rooting rate.
Embodiment
Below in conjunction with accompanying drawing the specific embodiment of the present invention is made a detailed explanation.
Embodiment 1:
The artificial cultivation method of a kind of radix tetrastigme comprises the steps:
1) select robust growth, physically well develop, do not have 1~2 year livings branch of damage by disease and insect, the plugged ear of 4 joints of length, 2 leaves in reservation top, the lower cut of plugged ear is cut into a smooth scarf, the ABT of 500mg/L (root-inducing powder) solution soaking 1h.
2) select the farmland sandy loam, plugged ear is become 35 °~45 oblique cutting with ground, the degree of depth is bottom 2 joints, cuttage finishes the compacting and watering immediately a little of matrix on every side, satisfy the demand of plugged ear to moisture, and plugged ear is fully contacted with matrix, it is moistening to keep inserting earth after the cuttage within the 15d, keep shading ventilation, the cuttage rooting survival rate reaches more than 90% behind the 30d.
3) the annual 3-4 month is transplanted into the field with cuttage seeding, and planting density is advisable with seeding row spacing 20cm * 30cm, adopts the sunshade net to cover during the cultivation, shield coverage is advisable with 70%, use composite fertilizer 30Kg/ mu after transplanting 15d, adopt the foliage spray of 100mg/L calcium nitrate solution simultaneously, amount of application 500L/ mu.Per mu yield can reach more than the 200Kg after 12 months.

Claims (5)

1. the artificial cultivation method of a radix tetrastigme is characterized in that, comprises the steps:
1) select robust growth, physically well develop, do not have 1~2 year livings branch of damage by disease and insect, the plugged ear of 4 joints of length keeps 2 leaves in top, the lower cut of plugged ear is cut into a smooth scarf, with the rooting powder solution immersion of 500mg/L;
2) select the farmland sandy loam, plugged ear is become 35 °~45 oblique cutting with ground, the degree of depth is bottom 2 joints, and cuttage finishes the compacting and watering immediately a little of matrix on every side, keeps slotting earth moistening after the cuttage within 15 days, the maintenance ventilation of shading;
3) annual 3~April cuttage seeding is transplanted into the field, the control planting density adopts the sunshade net to cover during the cultivation, transplant and use calcareous fertilisers after 15 days.
2. the artificial cultivation method of a kind of radix tetrastigme according to claim 1 is characterized in that, plugged ear was soaked 1 hour with the rooting powder solution of 500mg/L in the described step 1).
3. the artificial cultivation method of a kind of radix tetrastigme according to claim 1 is characterized in that, described step 2) in sunshade net shield coverage be 70%.
4. the artificial cultivation method of a kind of radix tetrastigme according to claim 1 is characterized in that, planting density is seeding row spacing 20cm * 30cm in the described step 3).
5. the artificial cultivation method of a kind of radix tetrastigme according to claim 1 is characterized in that, uses composite fertilizer 30Kg/ mu in the described step 3), adopts the foliage spray of 100mg/L calcium nitrate solution simultaneously, amount of application 500L/ mu.
CN2010106066555A 2010-12-27 2010-12-27 Artificial cultivation method of hemsley rockvine root CN102119625B (en)

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CN102726203A (en) * 2012-07-20 2012-10-17 丽水市莲都区绿谷三叶青珍稀植物研究所 Method for propagating radix tetrastigme underground officinal tubers
CN102919013A (en) * 2012-10-31 2013-02-13 丽水市汉方生物科技有限公司 High-efficiency imitative wild cultivation method for Tetrastigma hemsleyanum Diels et Gilg
CN103004436A (en) * 2012-12-18 2013-04-03 胡馨尹 Method for planting tetrastigma hemsleyanum dielset gilg
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CN103988699A (en) * 2014-05-30 2014-08-20 杭州中泽生物科技有限公司 Radix tetrastigme facility and cultivation method
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CN104429224A (en) * 2014-12-18 2015-03-25 广西壮族自治区药用植物园 Method for interrupting dormancy of radix tetrastigme seeds
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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107567918A (en) * 2017-09-08 2018-01-12 黄能 The artificial cultivation method of Erythropalum Scandens Blume

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101103700B (en) * 2007-08-06 2010-08-04 浙江大学 Tissue culture quick reproduction method of radix tetrastigmae
CN101822166A (en) * 2010-05-24 2010-09-08 董爱兰 Artificial cultivation method of gold thread hanging calabash

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101103700B (en) * 2007-08-06 2010-08-04 浙江大学 Tissue culture quick reproduction method of radix tetrastigmae
CN101822166A (en) * 2010-05-24 2010-09-08 董爱兰 Artificial cultivation method of gold thread hanging calabash

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
《上饶日报》 20060908 叶赛洋等 人工栽培三叶青在弋阳喜获成功 1 1-5 , 2 *
《植物生理学通讯》 20080229 钱丽华 三叶青的离体快速繁殖 121-122 1-5 第44卷, 第1期 2 *
《浙江中医杂志》 20070630 钟毓倩 三叶青的组织培养与快速繁殖研究 363 1-5 第42卷, 第6期 2 *

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