CN104429224B - One is broken the seed dormant method of radix tetrastigme - Google Patents
One is broken the seed dormant method of radix tetrastigme Download PDFInfo
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Abstract
The invention belongs to agricultural plant cultivation technique field, specially refer to one and break the seed dormant method of radix tetrastigme, comprise pretreated radix tetrastigme seed is soaked in seed soaking agent completely, the active ingredient of described seed soaking agent and proportioning thereof are: gibberellin 50-200mg/L, spirit hair element 10-50mg/L, NAA 110-280mg/L and NaOH 3-5g/L, all the other are water, soak time is 1-24 hour; Seed after soaking is put into and in constant incubator, carries out illumination and dark alternate culture. The present invention can remove radix tetrastigme seed dormancy fast; significantly improve its percentage of seedgermination; sprout neat; seedling stalwartness; the inventive method is easy and simple to handle simultaneously, cost is low, practical, pollution-free and easily popularization; for breeding and the large-scale planting of radix tetrastigme provide sound assurance, there is good economic benefit and social benefit.
Description
Technical field
The invention belongs to field of plant growing technology, being specifically related to one, to break radix tetrastigme seed dormantMethod.
Background technology
Radix tetrastigme is Vitaceae Tetrastigma plant tetratigma hemsleyanum (TetrastigmahemsleyanumDielsetGilg), have another name called stone monkey, stone mouse, hemsley rockvine root, gold thread hoist, silk thread and hang Jin Zhong, threeThe flat rattan of leaf, little flat rattan, soil are through ball etc. Radix tetrastigme is with piece root or all herbal medicine, for strong, precious jade is among the people conventionalChinese herbal medicine, bitter, pungent, cool in nature. Have antiviral, anti-inflammatory, analgesia and antipyretic, expelling wind and activating blood flow,The effect such as protecting the liver, is Western medicine irreplaceable " bouvardin ". Radix tetrastigme is distributed in China Guangxi, wideThe provinces and regions such as east, Jiangsu, Zhejiang, Fujian, mainly originate in Guangxi XiLin, Tianlin County, work in peace and contentment, reach the clouds,Jingxi, Long Lin, Yizhou, Huan Jiang, Luo Cheng, Nandan, ground such as peace, melt water, Quanzhou all.
Radix tetrastigme is rare and endangered medicinal plant, and Regional Distribution is scattered and reserves are rare. Along with three leavesGreen grass or young crops be primary raw material the series of products such as antineoplastic Chinese traditional medicine " golden stilbene sheet ", " spun gold ground capsule " constantlyExploitation, the market demand of radix tetrastigme is soaring year by year, and wild resource suffers excessively to excavate, and is on the verge of exhaustion.Radix tetrastigme mainly relies on seed to breed, but the seed amount of radix tetrastigme is very rare, and exists notDormancy characteristic, under nature, sprout time is long, germination percentage is low, gives scientific research and introducing and planting bandCarry out greatly difficulty. Current domestic prior art is for breaking the seed dormant research of radix tetrastigme.
Summary of the invention
One object of the present invention is to solve at least the problems referred to above or defect, and will after providing at leastThe advantage illustrating.
A further object of the present invention is to provide a kind of seed soaking agent, and it can efficient solution remove radix tetrastigme seed notThe various factors of sleeping, improves radix tetrastigme seed water absorbing capacity, finally promotes seed breaking dormancy, improves and plantsSub-germination percentage.
A further object of the present invention is to provide the method by water seed soaking processing and constant temperature culture, its combinationSeed soaking agent has further improved again the germination percentage of radix tetrastigme seed.
A further object of the invention is to provide one and breaks the seed dormant method of radix tetrastigme, and it operates letterSingle, cost is low, environmentally friendly, has stronger practicality and promotional value.
In order to realize according to these objects of the present invention and other advantage, provide one to break radix tetrastigme kindThe method of sub-dormancy, is soaked in pretreated radix tetrastigme seed in seed soaking agent described seed soaking agent completelyActive ingredient and proportioning thereof be: gibberellin 50-200mg/L, spirit hair element 10-50mg/L, NAA110-280mg/L and NaOH 3-5g/L, all the other are water, soak time is 1-24 hour.
Preferably, described breaks in the seed dormant method of radix tetrastigme, effective one-tenth of described seed soaking agentPoint and proportioning be: gibberellin 100mg/L, spirit hair element 40mg/L, NAA 115mg/L and hydrogen-oxygenChange sodium 4g/L, all the other are water, and invading the bubble time is 12 hours.
Preferably, described breaks in the seed dormant method of radix tetrastigme, before soaking radix tetrastigme seedNeed to anticipate radix tetrastigme seed, comprise the following steps:
Step 1, gather ripe radix tetrastigme fruit, rub with the hands and remove pericarp, clean, therefrom select full, nothingDamaged seed is for subsequent use;
It is that 2% liquor natrii hypochloritis soaks that step 2, the seed that described step 1 is selected are placed in mass fractionSteep 25 minutes, then to be placed in mass fraction be that 1% hydrogenperoxide steam generator soaks 30 minutes distilled water flushing5 times; And
Step 3, the seed that described step 2 was disinfected are put into warm water and are soaked 24 hours, warm waterTemperature is 35-70 DEG C.
Preferably, described breaks in the seed dormant method of radix tetrastigme, and described warm water temperature is 45 DEG C.
Preferably, described breaks in the seed dormant method of radix tetrastigme, also comprises three after soakingThe blue or green seed of leaf is placed on two-layer fully moistening sprouting paper, then described sprouting paper is put in to clear glass trainingSupport in ware cover lid; Again described clear glass culture dish is put in constant incubator, carry out illumination andDark alternate culture, described constant incubator temperature is 18-28 DEG C, described constant incubator humidity is60～70%, incubation time is 30 days.
Preferably, described breaks in the seed dormant method of radix tetrastigme, described constant incubator temperatureIt is 23 DEG C.
Preferably, described breaks in the seed dormant method of radix tetrastigme, described illumination and alternately darkCultivation is divided into three phases: the first stage is illumination 4 hours, and intensity of illumination is 1750-2050lx and dark trainingSupport 3 hours; Second stage is illumination 5 hours, and intensity of illumination is 2050-3500lx and dark cultivation 3 hours;Phase III is illumination 6 hours, and intensity of illumination is 3500-4500lx and dark cultivation 3 hours;
Wherein, the constant incubator humidity of described first stage is 60%, the constant temperature training of described second stageSupporting case humidity is 65%, and the constant incubator humidity of described phase III is 70%.
Preferably, described breaks in the seed dormant method of radix tetrastigme, described illumination and alternately darkCultivation is: the first stage is illumination 4 hours, and intensity of illumination is 2000lx and dark cultivation 3 hours; SecondStage is illumination 5 hours, and intensity of illumination is 3000lx and dark cultivation 3 hours; Phase III is illumination 6Hour, intensity of illumination is 4000lx and dark cultivation 3 hours.
The present invention at least comprises following beneficial effect::
1, the invention provides a kind of seed soaking agent, it can efficient solution remove the seed dormant various factors of radix tetrastigme,Improve radix tetrastigme seed water absorbing capacity, finally promote seed breaking dormancy, improve percentage of seedgermination.
2, the invention provides the method by water seed soaking processing and constant temperature culture, it enters again one in conjunction with seed soaking agentStep has improved the germination percentage of radix tetrastigme seed.
3, the method for illumination provided by the invention and dark alternate culture, while carrying out difference to radix tetrastigme seedBetween different light, also effectively removed the dormancy of radix tetrastigme seed.
4, the invention provides one and break the seed dormant method of radix tetrastigme, it is simple to operate, cost is low,Environmentally friendly, there is stronger practicality and promotional value.
Detailed description of the invention
Below in conjunction with embodiment, technical scheme of the present invention is described in further detail, to make this areaTechnical staff with reference to description word can according to enforcement.
The radix tetrastigme seed that the present invention tests use is the maturation in the collection in November of Xilin County, City: A Case Study in BaiseRadix tetrastigme seed.
Wherein, percentage of seedgermination refers to germination test final period, the whole seeds that germinate within the date of regulationNumber accounts for for planting experimentally sub percentage. In germination percentage (%)=fixed date chitting piece/confession plant experimentally seed number ×100％。
Embodiment 1
One is broken the seed dormant method of radix tetrastigme, comprises the following steps:
Before step a, immersion radix tetrastigme seed, need to anticipate radix tetrastigme seed, by ripe radix tetrastigmeFruit, rubs with the hands and removes pericarp, cleans, and therefrom selects full, unabroken seed for subsequent use; By the seed of selectingBe placed in mass fraction and be 2% liquor natrii hypochloritis and soak 25 minutes, then to be placed in mass fraction be 1% mistakeHydrogen peroxide solution is soaked 30 minutes, distilled water flushing 5 times; The seed of disinfecting is put into 45 DEG CIn warm water, soak 24 hours.
Step b, pretreated radix tetrastigme seed is divided into two parts, is aly immersed in phase as blankIn the water of same volume, another part is soaked in seed soaking agent completely, the active ingredient of described seed soaking agent and joiningThan being: gibberellin 100mg/L, spirit hair element 40mg/L, NAA 115mg/L and NaOH 4g/L,All the other are water, soak 12 hours.
Step c, by the radix tetrastigme seed of blank and soak after radix tetrastigme seed be placed in respectively two-layer fillingDivide on moistening sprouting paper, then described sprouting paper is put in clear glass culture dish to cover lid;Again described clear glass culture dish is put in and in constant incubator, carries out illumination and dark alternate culture, described inConstant incubator temperature is 23 DEG C, and described illumination and dark alternate culture are divided into three phases: the first stageFor illumination 4 hours, intensity of illumination was 2000lx and dark cultivation 3 hours; Second stage is illumination 5 hours,Intensity of illumination is 3000lx and dark cultivation 3 hours; Phase III is illumination 6 hours, and intensity of illumination is4000lx and dark cultivation 3 hours;
Wherein, the constant incubator humidity of described first stage is 60%, the constant temperature training of described second stageSupporting case humidity is 65%, and the constant incubator humidity of described phase III is 70%.
Incubation time is 30 days, after 30 days, adds up germination percentage.
In embodiment 2 to embodiment 9, the active ingredient of seed soaking agent and proportioning thereof are carried out to different selections,All the other steps are identical with above-described embodiment 1, the seed soaking agent in embodiment 2 to embodiment 9 effectivelyIn composition and proportioning difference table 1 below thereof, show. Statistics Implementation example 2 to embodiment 9 after 30 daysGermination percentage.
The germination percentage of embodiment 1 to embodiment 9 shows in table 1 below seeing.
Table 1: seed soaking agent active ingredient and proportioning thereof and corresponding germination percentage.
Find from the data analysis of table 1, seed soaking agent has significant impact to the germination percentage of radix tetrastigme seed,Wherein best seed soaking agent solution ratio is: gibberellin 100mg/L, spirit hair element 40mg/L, NAA115mg/L, NaOH 4g/L, the germination rate under this condition is up to 95%, far above blankGermination percentage (33%).
Embodiment 10
One is broken the seed dormant method of radix tetrastigme, comprises the following steps:
Before step a, immersion radix tetrastigme seed, need to anticipate radix tetrastigme seed, by ripe radix tetrastigmeFruit, rubs with the hands and removes pericarp, cleans, and therefrom selects full, unabroken seed for subsequent use; By the seed of selectingBe placed in mass fraction and be 2% liquor natrii hypochloritis and soak 25 minutes, then to be placed in mass fraction be 1% mistakeHydrogen peroxide solution is soaked 30 minutes, distilled water flushing 5 times; The seed of disinfecting is put into 35 DEG CIn warm water, soak 24 hours.
Step b, pretreated radix tetrastigme seed is divided into two parts, is aly immersed in phase as blankIn the water of same volume, another part is soaked in seed soaking agent completely, the active ingredient of described seed soaking agent and joiningThan being: gibberellin 100mg/L, spirit hair element 40mg/L, NAA 115mg/L and NaOH 4g/L,All the other are water, soak 12 hours.
Step c, by the radix tetrastigme seed of blank and soak after radix tetrastigme seed be placed in respectively two-layer fillingDivide on moistening sprouting paper, then described sprouting paper is put in clear glass culture dish to cover lid;Again described clear glass culture dish is put in and in constant incubator, carries out illumination and dark alternate culture, described inConstant incubator temperature is 23 DEG C, and described illumination and dark alternate culture are divided into three phases: the first stageFor illumination 4 hours, intensity of illumination was 2000lx and dark cultivation 3 hours; Second stage is illumination 5 hours,Intensity of illumination is 3000lx and dark cultivation 3 hours; Phase III is illumination 6 hours, and intensity of illumination is4000lx and dark cultivation 3 hours;
Wherein, the constant incubator humidity of described first stage is 60%, the constant temperature training of described second stageSupporting case humidity is 65%, and the constant incubator humidity of described phase III is 70%.
Incubation time is 30 days, after 30 days, adds up germination percentage.
In embodiment 11 to embodiment 13, the temperature of emerge in worm water is carried out to different selections, all the other stepsRapid identical with above-described embodiment 10, the different warm water temperature in embodiment 11 to embodiment 13 dividesIn table 2 not below, show.
The germination percentage of Statistics Implementation example 11 to embodiment 13 after 30 days.
The germination percentage of embodiment 10 to embodiment 13 shows in table 2 below seeing.
Table 2: different warm water temperatures and corresponding germination percentage.
Above-mentioned test contrasts selection to the emerge in worm water temperature of pretreatment radix tetrastigme seed, from above-mentioned table2 data that show are known, and this step of emerge in worm water has significant shadow to the germination percentage of radix tetrastigme seedRing, although the temperature difference in embodiment 11 to embodiment 13 at best seed soaking agent solution ratio is:Gibberellin 100mg/L, spirit hair element 40mg/L, NAA 115mg/L, under NaOH 4g/L conditionMinimum germination percentage be 74%, still higher than blank germination percentage (33%), but embodiment 11Germination percentage to embodiment 13 is still the germination percentage 95% of the embodiment 1 of 45 DEG C lower than warm water temperature,It is best warm water temperature that final selection warm water temperature is 45 DEG C.
Embodiment 14
One is broken the seed dormant method of radix tetrastigme, comprises the following steps:
Before step a, immersion radix tetrastigme seed, need to anticipate radix tetrastigme seed, by ripe radix tetrastigmeFruit, rubs with the hands and removes pericarp, cleans, and therefrom selects full, unabroken seed for subsequent use; By the seed of selectingBe placed in mass fraction and be 2% liquor natrii hypochloritis and soak 25 minutes, then to be placed in mass fraction be 1% mistakeHydrogen peroxide solution is soaked 30 minutes, distilled water flushing 5 times; The seed of disinfecting is put into 45 DEG CIn warm water, soak 24 hours.
Step b, pretreated radix tetrastigme seed is divided into two parts, is aly immersed in phase as blankIn the water of same volume, another part is soaked in seed soaking agent completely, the active ingredient of described seed soaking agent and joiningThan being: gibberellin 100mg/L, spirit hair element 40mg/L, NAA 115mg/L and NaOH 4g/L,All the other are water, soak 12 hours.
Step c, by the radix tetrastigme seed of blank and soak after radix tetrastigme seed be placed in respectively two-layer fillingDivide on moistening sprouting paper, then described sprouting paper is put in clear glass culture dish to cover lid;Again described clear glass culture dish is put in and in constant incubator, carries out illumination and dark alternate culture, described inConstant incubator temperature is 23 DEG C, and described illumination and dark alternate culture are divided into three phases: the first stageFor illumination 4 hours, intensity of illumination was 1750lx and dark cultivation 3 hours; Second stage is illumination 5 hours,Intensity of illumination is 2050lx and dark cultivation 3 hours; Phase III is illumination 6 hours, and intensity of illumination is3500lx and dark cultivation 3 hours;
Wherein, the constant incubator humidity of described first stage is 60%, the constant temperature training of described second stageSupporting case humidity is 65%, and the constant incubator humidity of described phase III is 70%.
Incubation time is 30 days, after 30 days, adds up germination percentage.
In embodiment 15 to embodiment 17, the illumination during insulating box is cultivated and the bar of dark alternate culturePart changes, and all the other steps are identical with above-described embodiment 1, in embodiment 15 to embodiment 17Illumination and the condition of dark alternate culture in table 3 below, show respectively.
After 30 days, the germination percentage of Statistics Implementation example 15 to embodiment 17.
The germination percentage of embodiment 15 to embodiment 17 shows in table 3 below seeing.
Table 3: the different condition of illumination and dark alternate culture and corresponding germination percentage.
Illumination and the dark alternate culture condition of above-mentioned test to radix tetrastigme seed contrasts selection, from upperState the data that table 3 shows known, illumination and the germination of this step of dark alternate culture to radix tetrastigme seedRate has significant impact, although the illumination condition in embodiment 14 to embodiment 17 is different,Good seed soaking agent solution ratio is: gibberellin 100mg/L, and spirit hair element 40mg/L, NAA 115mg/L,Germination percentage under NaOH 4g/L condition is minimum is 75%, still higher than blank germination percentage(33%), but the germination percentage in embodiment 14 to embodiment 17 or lower than the germination percentage of embodiment 195%, final selection is that described illumination and dark alternate culture are: the first stage is illumination 4 hours, illuminationIntensity is 2000lx and dark cultivation 3 hours; Second stage is illumination 5 hours, and intensity of illumination is 3000lxWith dark cultivation 3 hours; Phase III is illumination 6 hours, and intensity of illumination is that 4000lx and dark cultivation 3 are littleTime be best illumination and dark alternate culture condition.
The present invention has also been contrast test A, contrast test B and contrast test C is as follows:
Contrast test A
One is broken the seed dormant method of radix tetrastigme, comprises the following steps:
Before step a, immersion radix tetrastigme seed, need to anticipate radix tetrastigme seed, by ripe radix tetrastigmeFruit, rubs with the hands and removes pericarp, cleans, and therefrom selects full, unabroken seed for subsequent use; By the seed of selectingBe placed in mass fraction and be 2% liquor natrii hypochloritis and soak 25 minutes, then to be placed in mass fraction be 1% mistakeHydrogen peroxide solution is soaked 30 minutes, distilled water flushing 5 times;
Step b, pretreated radix tetrastigme seed is divided into two parts, is aly immersed in phase as blankIn the water of same volume, another part is soaked in seed soaking agent completely, the active ingredient of described seed soaking agent and joiningThan being: gibberellin 100mg/L, spirit hair element 40mg/L, NAA 115mg/L and NaOH 4g/L,All the other are water, soak 12 hours.
Step c, by the radix tetrastigme seed of blank and soak after radix tetrastigme seed be placed in respectively two-layer fillingDivide on moistening sprouting paper, then described sprouting paper is put in clear glass culture dish to cover lid;Again described clear glass culture dish is put in constant incubator and is cultivated, described constant incubator temperature is 23DEG C, incubation time is 30 days.
After 30 days, adding up germination percentage is 70%, although than germination percentage (33%) height of blank test,But still there is no germination percentage (95%) height in embodiment 1.
Contrast test B
One is broken the seed dormant method of radix tetrastigme, comprises the following steps:
Before step a, immersion radix tetrastigme seed, need to anticipate radix tetrastigme seed, by ripe radix tetrastigmeFruit, rubs with the hands and removes pericarp, cleans, and therefrom selects full, unabroken seed for subsequent use; By the seed of selectingBe placed in mass fraction and be 2% liquor natrii hypochloritis and soak 25 minutes, then to be placed in mass fraction be 1% mistakeHydrogen peroxide solution is soaked 30 minutes, distilled water flushing 5 times; The seed of disinfecting is put into 35 DEG CIn warm water, soak 24 hours.
Step b, pretreated radix tetrastigme seed is divided into two parts, is aly immersed in phase as blankIn the water of same volume, another part is soaked in seed soaking agent completely, the active ingredient of described seed soaking agent and joiningThan being: gibberellin 100mg/L, spirit hair element 40mg/L, NAA 115mg/L and NaOH 4g/L,All the other are water, soak 12 hours.
Step c, by the radix tetrastigme seed of blank and soak after radix tetrastigme seed be placed in respectively two-layer fillingDivide on moistening sprouting paper, then described sprouting paper is put in clear glass culture dish to cover lid;Again described clear glass culture dish is put in constant incubator and is cultivated, described constant incubator temperatureBe 23 DEG C, incubation time is 30 days.
After 30 days, adding up germination percentage is 80%, although than germination percentage (33%) height of blank test,But still there is no germination percentage (95%) height in embodiment 1.
Contrast test C
One is broken the seed dormant method of radix tetrastigme, comprises the following steps:
Before step a, immersion radix tetrastigme seed, need to anticipate radix tetrastigme seed, by ripe radix tetrastigmeFruit, rubs with the hands and removes pericarp, cleans, and therefrom selects full, unabroken seed for subsequent use; By the seed of selectingBe placed in mass fraction and be 2% liquor natrii hypochloritis and soak 25 minutes, then to be placed in mass fraction be 1% mistakeHydrogen peroxide solution is soaked 30 minutes, distilled water flushing 5 times;
Step b, pretreated radix tetrastigme seed is divided into two parts, is aly immersed in as blankIn the water of same volume, another part is soaked in seed soaking agent completely, the active ingredient of described seed soaking agent andProportioning is: gibberellin 100mg/L, spirit hair element 40mg/L, NAA 115mg/L and NaOH4g/L, all the other are water, soak 12 hours.
Step c, by the radix tetrastigme seed of blank and soak after radix tetrastigme seed be placed in respectively two-layer fillingDivide on moistening sprouting paper, then described sprouting paper is put in clear glass culture dish to cover lid;Again described clear glass culture dish is put in and in constant incubator, carries out illumination and dark alternate culture, described inConstant incubator temperature is 23 DEG C, and described illumination and dark alternate culture are divided into three phases: the first stageFor illumination 4 hours, intensity of illumination was 2000lx and dark cultivation 3 hours; Second stage is illumination 5 hours,Intensity of illumination is 3000lx and dark cultivation 3 hours; Phase III is illumination 6 hours, and intensity of illumination is4000lx and dark cultivation 3 hours;
Wherein, the constant incubator humidity of described first stage is 60%, the constant temperature training of described second stageSupporting case humidity is 65%, and the constant incubator humidity of described phase III is 70%.
Incubation time is 30 days.
After 30 days, adding up germination percentage is 85%, although than germination percentage (33%) height of blank test,But still there is no germination percentage (95%) height in embodiment 1.
The present invention adopts the seed soaking agent seed soaking of autogamy, can efficient solution except radix tetrastigme seed dormant eachThe factor of kind, comprises seed coat obstacle and germination inhibitor etc., improves radix tetrastigme seed water absorbing capacity, finally shortEnter seed breaking dormancy, improve seed germination rate, and the present invention is environmentally friendly, have operation letter simultaneously concurrentlyJust, advantage that cost is low, there is stronger practicality and promotional value, and utilize water seed soaking placeReason and the method for constant temperature culture, further improve the germination rate of radix tetrastigme seed.
The invention belongs to first breaking the achievement in research of radix tetrastigme seed dormancy technology, remove fast radix tetrastigmeSeed dormancy, significantly improved its seed germination rate, simultaneously the inventive method is easy and simple to handle, cost is low,Practical, pollution-free and easily promote, for breeding and the large-scale planting of radix tetrastigme provide strong guarantorBarrier, has good economic benefit and social benefit.
Although embodiment of the present invention are open as above, it is not restricted to description and enforcement sideListed utilization in formula, for those skilled in the art, can easily realize other amendment, because ofThis is not deviating under the universal that claim and equivalency range limit, and the present invention is not limited to specificDetails and illustrate here and the example of describing.
Claims (6)
1. break the seed dormant method of radix tetrastigme, it is characterized in that, comprise the following steps:
Step 1, gather ripe radix tetrastigme fruit, rub with the hands and remove pericarp, clean, therefrom select full, unabroken seed for subsequent use;
It is that 2% liquor natrii hypochloritis soaks 25 minutes that step 2, the seed that described step 1 is selected are placed in mass fraction, then to be placed in mass fraction be that 1% hydrogenperoxide steam generator soaks 30 minutes, distilled water flushing 5 times;
Step 3, the seed that described step 2 was disinfected are put into warm water and are soaked 24 hours, and warm water temperature is 35-70 DEG C;
Step 4, radix tetrastigme seed pretreated described step 3 is soaked in seed soaking agent completely, the active ingredient of described seed soaking agent and proportioning thereof are: gibberellin 50-200mg/L, spirit hair element 10-50mg/L, NAA 110-280mg/L and NaOH 3-5g/L, all the other are water, and soak time is 1-24 hour;
Step 5, the radix tetrastigme seed after soaking is placed on two-layer fully moistening sprouting paper, then described sprouting paper is put in clear glass culture dish to cover lid; Again described clear glass culture dish is put in constant incubator and carries out illumination and dark alternate culture, described constant incubator temperature is 18-28 DEG C, and described constant incubator humidity is 60～70%, and incubation time is 30 days.
2. the seed dormant method of radix tetrastigme of breaking as claimed in claim 1, is characterized in that, the active ingredient of described seed soaking agent and proportioning thereof are: gibberellin 100mg/L, spirit hair element 40mg/L, NAA 115mg/L and NaOH 4g/L, all the other are water, soak time is 12 hours.
3. the seed dormant method of radix tetrastigme of breaking as claimed in claim 1, is characterized in that, described warm water temperature is 45 DEG C.
4. the seed dormant method of radix tetrastigme of breaking as claimed in claim 1, is characterized in that, described constant incubator temperature is 23 DEG C.
5. the seed dormant method of radix tetrastigme of breaking as claimed in claim 1, is characterized in that, described illumination and dark alternate culture are divided into three phases: the first stage is illumination 4 hours, and intensity of illumination is 1750-2050lx and dark cultivation 3 hours; Second stage is illumination 5 hours, and intensity of illumination is 2050-3500lx and dark cultivation 3 hours; Phase III is illumination 6 hours, and intensity of illumination is 3500-4500lx and dark cultivation 3 hours;
Wherein, the constant incubator humidity of described first stage is 60%, and the constant incubator humidity of described second stage is 65%, and the constant incubator humidity of described phase III is 70%.
6. the seed dormant method of radix tetrastigme of breaking as claimed in claim 5, is characterized in that, described illumination and dark alternate culture are: the first stage is illumination 4 hours, and intensity of illumination is 2000lx and dark cultivation 3 hours; Second stage is illumination 5 hours, and intensity of illumination is 3000lx and dark cultivation 3 hours; Phase III is illumination 6 hours, and intensity of illumination is 4000lx and dark cultivation 3 hours.
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| CN105052304B (en) * | 2015-08-13 | 2017-07-21 | 云南省农业科学院甘蔗研究所 | A kind of method for promoting the old hybrid seed of sugarcane to sprout |
| CN107637215A (en) * | 2017-10-17 | 2018-01-30 | 吴小良 | Break the method for ardisia gigantifolia Stapf seed dormancy |
| CN107615939A (en) * | 2017-10-17 | 2018-01-23 | 吴小良 | Break the method for centering rattan seed dormancy |
| CN112789965A (en) * | 2021-01-14 | 2021-05-14 | 上饶师范学院 | Method for promoting germination of radix tetrastigme seeds |
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