CN104025762B - A kind of method promoting purple striae lip post lettuce tongue seed germination - Google Patents
A kind of method promoting purple striae lip post lettuce tongue seed germination Download PDFInfo
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- CN104025762B CN104025762B CN201410244221.3A CN201410244221A CN104025762B CN 104025762 B CN104025762 B CN 104025762B CN 201410244221 A CN201410244221 A CN 201410244221A CN 104025762 B CN104025762 B CN 104025762B
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Abstract
The invention discloses a kind of method promoting purple striae lip post lettuce tongue seed germination.The method to be germinateed the purple striae lip post lettuce tongue seed cultivated and obtain sprouting by the sterilization of the collection of (1) seed, the cleaning of (2) seed, (3) seed, (4) seed soaking and (5).Benefit effect of the present invention: 1. the inventive method is simple to operate, cost is low, be easy to manual operation, be easy to the advantages such as popularization, is suitable for production application.2. the inventive method improves the germination rate of purple striae lip post lettuce tongue seed significantly; the seed germination time ahead of time; improve the regularity of seed germination, for the sexual propagation of purple striae lip post lettuce tongue provides good basis, be applicable to the scaled artificial planting of purple striae lip post lettuce tongue.
Description
Technical field
The invention belongs to field of plant growing technology, be specifically related to a kind of method promoting purple striae lip post lettuce tongue seed germination.
Background technology
Purple striae lip post lettuce tongue (ChiritapseudoeburneaW.T.Wang) is Gesneriaceae (Gesneriaceae) Chirita (Chirita) herbaceos perennial, for Guangxi endemic species, only there is distribution in Tiandong County, Guangxi, be born on limestone.Be used as medicine with root, have effect of preventing phlegm from forming and stopping coughing, be used for the treatment of asthma.Meanwhile, purple striae lip post lettuce tongue purple, corolla is comparatively large, and the fleshy leaf of Ye Weihou, has very high ornamental value.For a long time, purple striae lip post lettuce tongue medicinal material all depends on wild plant resource, excessively unorderedly for a long time excavates, and its wild resource is reduced gradually and even faces extinction risk.
Purple striae lip post lettuce tongue grain weight is large, seed storage, convenient transportation, adopts the mode of seeding and seedling raising can obtain a large amount of seedling, is applicable to large-area seedling breeding.But; superfine little (the seed ellipse of purple striae lip post lettuce tongue seed; long 0.40 ~ 0.55mm; wide 0.14 ~ 0.22mm); and seed exists hibernation feature, the percentage of seedgermination of newly gathering is only 35%, seriously hinders popularization and the development of large area artificial planting; therefore, be badly in need of carrying out protection of resources and the research of artificial seedling quick propagating technology to it.
Summary of the invention
The object of the present invention is to provide a kind of method promoting purple striae lip post lettuce tongue seed germination, remove purple striae lip post lettuce tongue seed dormancy, promote seed germination, for the seedling breeding of purple striae lip post lettuce tongue lays the foundation.
The technical scheme that the present invention solves the problems of the technologies described above is as follows:
Promote a method for purple striae lip post lettuce tongue seed germination, operating procedure is as follows:
1. the collection of seed: annual July, when purple striae lip post lettuce tongue capsule browning look, when middle suture has a little cracking, adopts down capsule, puts indoor shady and coolly dryly to locate, after capsule natural cracking, collects seed;
2. the cleaning of seed: the seed collected with two-layer square hole screen screening step (1) that sieve aperture is 0.15mm and 0.60mm, stays the seed clean for purple striae lip post lettuce tongue between two layer screen;
3. sterilize: the purple striae lip post lettuce tongue seed mass percentage concentration that step (2) is cleaned be 0.1% liquor potassic permanganate soak 5 minutes, then use distilled water flushing 3 times;
4. soak seed: the Gibberellins solution of step (3) through the seed 100 ~ 600mg/L disinfected is soaked, cleaner with distilled water flushing; The volume ratio of seed and Gibberellins solution is 1:3, soaking temperature 20 ~ 25 DEG C, seed soaking time 12 ~ 24 hours;
5. germinate and cultivate: step (4) is placed in through the purple striae lip post lettuce tongue seed of seed soaking the culture dish that diameter is 9cm, sprout under constant temperature and alternate illumination condition, using 24 hours as a cycle period, cultivate 30 days, purple striae lip post lettuce tongue seed germination; Described constant temperature and alternate illumination condition are: constant temperature 25 DEG C, and 0:00 ~ 12:00 illumination every day 1000 ~ 3000lx, 12:00 ~ 24:00 are dark.
Utilize preferred seed soaking measure of the present invention and germination condition, namely soak seed 12 hours with the Gibberellins solution of 400mg/L, again at 25 DEG C of constant temperature, doing under the condition of 0:00 ~ 12:00 illumination every day 1000 ~ 3000lx, 12:00 ~ 24:00 dark germinates cultivates, the sprout time first of purple striae lip post lettuce tongue seed is the 6th day, within 14th day, sprout and terminate, lasting duration only 8 days, final germination rate reaches more than 95%.The contrast seed not doing any process first sprout time is the 12nd day, and within the 30th day, sprout and terminate, lasting duration is 18 days, and final germination rate is only 35%.The preferred method of this patent makes the sprout time first of purple striae lip post lettuce tongue do sth. in advance 6 days, and the time span continuing to sprout shortens 10 days, and makes final germination rate add more than 60%.
Benefit effect of the present invention:
1. the inventive method is simple to operate, cost is low, be easy to manual operation, be easy to the advantages such as popularization, is suitable for production application.
2. the inventive method improves the germination rate of purple striae lip post lettuce tongue seed significantly; the seed germination time ahead of time; improve the regularity of seed germination, for the sexual propagation of purple striae lip post lettuce tongue provides good basis, be applicable to the scaled artificial planting of purple striae lip post lettuce tongue.
Embodiment
Below in conjunction with embodiment, the invention will be further described, but the present invention is not limited thereto.
Experimental technique described in following embodiment, if no special instructions, is conventional method; Described reagent and material, if no special instructions, all can obtain from commercial channels.
Embodiment 1
Promote the method for purple striae lip post lettuce tongue seed germination, operating procedure is as follows:
1. seed collection and cleaning: on July 10th, 2013, the purple striae lip post lettuce tongue capsule of brown of gathering in Medicinal Plants of Guangxi garden maturation, capsule is adopted lower after be positioned over indoor shady and cool dry place, after capsule season crack, screen with the two-layer square hole bushing screen that sieve aperture is 0.15mm and 0.60mm, the ash bits that removing bottom sifts out and the flat kind of superfine little sky, the non-seed parts such as the capsule shell simultaneously also removing top layer remnants and other withes, stay the clean seed 10g between two layer screen.
2. sterilize: 0.1% liquor potassic permanganate getting the purple striae lip post lettuce tongue seed 1g 10ml cleaned soaks 5 minutes, then rinses 3 times with clear water.
3. soak seed: soak 12 hours under the Gibberellins solution of the 400mg/L now joined through the purple striae lip post lettuce tongue seed 3ml that disinfects being placed in 25 DEG C of constant temperatures, with distilled water flushing 3 times after taking out.
4. germinate and cultivate: be JZ1 by the purple striae lip post lettuce tongue seed tag through Gibberellins solution seed soaking, compare with the clean seed without seed soaking, be labeled as CK.It is in the culture dish of 9cm that the seed of two process is evenly put in diameter respectively, and with two-layer qualitative filter paper for germinating bed, every ware puts 100, each repetition 4 times.Be placed in by culture dish in illumination box again and cultivate 30 days, regular moisturizing keeps filter paper moistening, and the temperature and light of illumination box is according to being set to 25 DEG C of constant temperature, and 0:00 ~ 12:00 illumination every day 1000 ~ 3000lx, 12:00 ~ 24:00 are dark.
5. data statistics: the percentage that the seed grain number breaking through seed coat with radicle accounts for for planting experimentally seed number is designated as percentage of seedgermination, gets the average repeated for 4 times.The seed of 400mg/L Gibberellins solution seed soaking JZ1 first sprout time is the 6th day, and within the 14th day, sprout and terminate, lasting duration is 8 days, and final germination rate is 95.7%.The seed of control treatment CK first sprout time is the 12nd day, and within the 30th day, sprout and terminate, lasting duration is 18 days, and final germination rate is 35%.This patent method makes the sprout time first of purple striae lip post lettuce tongue do sth. in advance 6 days, and the time span continuing to sprout shortens 10 days, and makes final germination rate add 60.7%.
Embodiment 2
Promote the method for purple striae lip post lettuce tongue seed germination:
This example operation process with seed collection described in embodiment 1, clean, sterilize, to sprout condition of culture identical, but soaking 18 hours under the Gibberellins solution that seed soaking operation is the 400mg/L now joined by the purple striae lip post lettuce tongue seed 3ml through disinfecting is placed in 22 DEG C of constant temperatures, taking out rear distilled water flushing 3 times.Be JZ2 by the purple striae lip post lettuce tongue seed tag through Gibberellins solution seed soaking.
The seed of Gibberellins solution seed soaking JZ2 first sprout time is the 8th day, and within the 18th day, sprout and terminate, lasting duration is 10 days, and final germination rate is 93.5%.Compared with the seed of the non-seed soaking of contrast, this patent method makes the sprout time first of purple striae lip post lettuce tongue do sth. in advance 4 days, and the time span continuing to sprout shortens 8 days, and makes final germination rate add 58.5%.
Embodiment 3
Promote the method for purple striae lip post lettuce tongue seed germination:
This example operation process with seed collection described in embodiment 1, clean, sterilize, to sprout condition of culture identical, but soaking 24 hours under the Gibberellins solution that seed soaking operation is the 200mg/L now joined by the purple striae lip post lettuce tongue seed 3ml through disinfecting is placed in 20 DEG C of constant temperatures, taking out rear distilled water flushing 3 times.Be JZ3 by the purple striae lip post lettuce tongue seed tag through Gibberellins solution seed soaking.
The seed of Gibberellins solution seed soaking JZ3 first sprout time is the 8th day, and within the 18th day, sprout and terminate, lasting duration is 10 days, and final germination rate is 93.7%.Compared with the seed of the non-seed soaking of contrast, this patent method makes the sprout time first of purple striae lip post lettuce tongue do sth. in advance 4 days, and the time span continuing to sprout shortens 8 days, and makes final germination rate add 58.7%.
Embodiment 4
Promote the method for purple striae lip post lettuce tongue seed germination:
This example operation process with seed collection described in embodiment 1, clean, sterilize, to sprout condition of culture identical, but seed soaking operation is the Gibberellins solution of the 100mg/L now joined through the purple striae lip post lettuce tongue seed 3ml that disinfects by 1g be placed in 25 DEG C of constant temperatures under soak 24 hours, with distilled water flushing 3 times after taking out.Be JZ4 by the purple striae lip post lettuce tongue seed tag through Gibberellins solution seed soaking.
The seed of Gibberellins solution seed soaking JZ4 first sprout time is the 7th day, and within the 20th day, sprout and terminate, lasting duration is 13 days, and final germination rate is 87.7%.Compared with the seed of the non-seed soaking of contrast, this patent method makes the sprout time first of purple striae lip post lettuce tongue do sth. in advance 5 days, and the time span continuing to sprout shortens 5 days, and makes final germination rate add 52.7%.
Embodiment 5
Promote the method for purple striae lip post lettuce tongue seed germination:
This example operation process with seed collection described in embodiment 1, clean, sterilize, to sprout condition of culture identical, but seed soaking operation is the Gibberellins solution of the 600mg/L now joined through the purple striae lip post lettuce tongue seed 3ml that disinfects by 1g be placed in 25 DEG C of constant temperatures under soak 12 hours, with distilled water flushing 3 times after taking out.Be JZ5 by the purple striae lip post lettuce tongue seed tag through Gibberellins solution seed soaking.
The seed of Gibberellins solution seed soaking JZ5 first sprout time is the 7th day, and within the 16th day, sprout and terminate, lasting duration is 9 days, and final germination rate is 81.0%.Compared with the seed of the non-seed soaking of contrast, this patent method makes the sprout time first of purple striae lip post lettuce tongue do sth. in advance 5 days, and the time span continuing to sprout shortens 9 days, and makes final germination rate add 46.0%.
Embodiment 6
Promote the method for purple striae lip post lettuce tongue seed germination:
This example operation process cleans with seed described in embodiment 1, sterilize, soak seed, to sprout condition of culture identical, but experiment material is the wild purple striae lip post lettuce tongue seed collected from Tiandong County, Guangxi Zhuang Autonomous Region in July, 2013.Be JZ6 by the purple striae lip post lettuce tongue seed tag through Gibberellins solution seed soaking, compare with the clean seed without seed soaking, be labeled as CK1.
The seed of Gibberellins solution seed soaking JZ6 first sprout time is the 7th day, and within the 14th day, sprout and terminate, lasting duration is 7 days, and final germination rate is 97.0%.The seed of wild species control treatment CK1 first sprout time is the 17th day, and within the 30th day, sprout and terminate, lasting duration is 13 days, and final germination rate is 29.0%.This patent method makes the sprout time first of purple striae lip post lettuce tongue do sth. in advance 10 days, and the time span continuing to sprout shortens 6 days, and makes final germination rate add 68.0%.
Claims (1)
1. promote a method for purple striae lip post lettuce tongue seed germination, it is characterized in that, operating procedure is as follows:
(1) collection of seed: annual July, when purple striae lip post lettuce tongue capsule browning look, when middle suture has a little cracking, adopts down capsule, puts indoor shady and coolly dryly to locate, after capsule natural cracking, collects seed;
(2) the cleaning of seed: the seed collected with two-layer square hole screen screening step (1) that sieve aperture is 0.15mm and 0.60mm, leaves the seed clean for purple striae lip post lettuce tongue between two layer screen;
(3) sterilization of seed: the purple striae lip post lettuce tongue seed mass percentage concentration that step (2) is cleaned be 0.1% liquor potassic permanganate soak 5 minutes, then use distilled water flushing 3 times;
(4) soak seed: step (3) is soaked through the Gibberellins solution of seed 400mg/L of disinfecting, then with distilled water flushing clean 3 times; The volume ratio of seed and Gibberellins solution is 1:3, soaking temperature 20 ~ 25 DEG C, seed soaking time 12 ~ 24 hours;
(5) germination is cultivated: step (4) is placed in through the purple striae lip post lettuce tongue seed of seed soaking the culture dish that diameter is 9cm, sprout under constant temperature and alternate illumination condition, using 24 hours as a cycle period, cultivate 30 days, purple striae lip post lettuce tongue seed germination; Described constant temperature and alternate illumination condition are: constant temperature 25 DEG C, and 0:00 ~ 12:00 illumination every day 1000 ~ 3000lx, 12:00 ~ 24:00 are dark.
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| CN104025762B true CN104025762B (en) | 2015-12-02 |
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| CN104541672B (en) * | 2015-01-06 | 2016-08-24 | 广西壮族自治区药用植物园 | A kind of method improving medicinal lip post lettuce tongue seed germination rate |
| CN113950892A (en) * | 2021-12-06 | 2022-01-21 | 中国科学院东北地理与农业生态研究所 | Pretreatment method for promoting germination of zoysia japonica seeds |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101120635A (en) * | 2007-09-07 | 2008-02-13 | 兰州大学 | Method for promoting nitraria seed quick germination |
| CN102119594A (en) * | 2010-12-13 | 2011-07-13 | 江苏省农业科学院 | Method for promoting rhododendron parvifolium seed germination |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101120635A (en) * | 2007-09-07 | 2008-02-13 | 兰州大学 | Method for promoting nitraria seed quick germination |
| CN102119594A (en) * | 2010-12-13 | 2011-07-13 | 江苏省农业科学院 | Method for promoting rhododendron parvifolium seed germination |
Non-Patent Citations (2)
| Title |
|---|
| 吊石苣苔和盾叶粗筒苣苔的种子萌发及染色体数目观察;赵大克等;《云南农业大学学报(自然科学版)》;20100331;第25卷(第2期);第173-177页 * |
| 外源激素处理对线叶唇柱苣苔种子萌发的影响;潘春柳等;《种子》;20110131;第30卷(第1期);第24-27页 * |
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Inventor after: Pan Chunliu Inventor after: Huang Yanfen Inventor after: Zhu Yanxia Inventor after: Dong Qingsong Inventor after: Ke Fang Inventor after: Jiu Jianhua Inventor before: Pan Chunliu Inventor before: Huang Yanfen Inventor before: Zhu Yanxia Inventor before: Dong Qingsong Inventor before: Ke Fang |
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