CN104472062A - Method for promoting cymbariae seeds to germinate - Google Patents
Method for promoting cymbariae seeds to germinate Download PDFInfo
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- CN104472062A CN104472062A CN201410844940.9A CN201410844940A CN104472062A CN 104472062 A CN104472062 A CN 104472062A CN 201410844940 A CN201410844940 A CN 201410844940A CN 104472062 A CN104472062 A CN 104472062A
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- fragrant plant
- plant seed
- core fragrant
- germination
- cymbariae
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- 238000000034 method Methods 0.000 title claims abstract description 32
- 230000001737 promoting effect Effects 0.000 title claims abstract description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 10
- 230000001954 sterilising effect Effects 0.000 claims abstract description 9
- 238000011081 inoculation Methods 0.000 claims abstract description 6
- 230000007226 seed germination Effects 0.000 claims description 22
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims description 5
- 239000000645 desinfectant Substances 0.000 claims description 4
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 4
- 230000001771 impaired effect Effects 0.000 claims description 3
- 239000004576 sand Substances 0.000 claims description 3
- 230000035784 germination Effects 0.000 abstract description 26
- 230000000694 effects Effects 0.000 abstract description 4
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 34
- 238000002474 experimental method Methods 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 208000032843 Hemorrhage Diseases 0.000 description 2
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- 239000003814 drug Substances 0.000 description 2
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- 238000011835 investigation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 244000296912 Ageratum conyzoides Species 0.000 description 1
- 235000004405 Ageratum conyzoides Nutrition 0.000 description 1
- 235000016535 Capraria biflora Nutrition 0.000 description 1
- 241000155170 Cymbaria mongolica Species 0.000 description 1
- 208000034507 Haematemesis Diseases 0.000 description 1
- 235000017309 Hypericum perforatum Nutrition 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 206010030124 Oedema peripheral Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 230000001139 anti-pruritic effect Effects 0.000 description 1
- 239000003908 antipruritic agent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 235000019636 bitter flavor Nutrition 0.000 description 1
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- 210000004369 blood Anatomy 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000012297 crystallization seed Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 208000035861 hematochezia Diseases 0.000 description 1
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Landscapes
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for promoting cymbariae seeds to germinate, belongs to the technical field of plant cultivation, and relates to a method for promoting plants to germinate by using a breeding seedling growing technique. The method comprises the following steps: damaging the episperm of soaked cymbariae seeds, and subsequently performing ordinary sterilization and inoculation as well as constant-temperature culture so as to promote the seeds to germinate. By the adoption of the method disclosed by the invention, germination of cymbariae seeds is effectively promoted, and the problems that the germination activity is poor and the germination rate is low are solved. The method further has the characteristics of simplicity in operation, low cost, high germination rate and easiness in popularization, and has very high application values in practical and effective on-scale planting, protection and continuous cymbariae resource utilization.
Description
Technical field
The invention belongs to field of plant growing technology, relating to the method being promoted plant germination by breeding and seedling technology, is a kind of method promoting core fragrant plant seed germination in particular.
Technical background
Anaesthetic core fragrant plant, anaesthetic name " I Tan-A gives " is goatweed Da Wuli core fragrant plant
cymbariadahuricaand Radix Cymbariae mongolicae L.
cymbariamongolicamaxim. dry herb is Mongolian medicine's medicine dedicated.Originate in the provinces and regions such as China Heilungkiang, the Inner Mongol, Hebei and be born in hillslope between height above sea level 620-1100 rice with on gravel, grassland.Its mildly bitter flavor, cool in nature, have dry " Xieri Wusu Symptom ", heat-clearing, pain relieving, the effect such as antipruritic, detumescence, hemostasis." national Chinese herbal medicine compilation " record core fragrant plant have dispel rheumatism, inducing diuresis to reduce edema, cooling blood and hemostasis effect, be mainly used in treatment rheumatism, two edema of lower extremity, yellow water blister, menorrhalgia, haematemesis, bleeding from five sense organs or subcutaneous tissue clinically, have blood in stool, the disease such as traumatism and bleeding.In recent years, due to the raising of core fragrant plant medical value, the market demand constantly increases, and causes transition to be excavated, and cause wild resource reserves increasingly to reduce, resource is on the verge of exhaustion.In order to alleviate the contradiction day by day increased between shortage of resources and exploitation, carrying out artificial planting is the most effective approach of one.Find in preliminary breeding and seedling work, core fragrant plant exists in seeding process that germination rate is low, the situation such as irregular of germinateing, and seriously governs standardized planting and the production of core fragrant plant.Therefore, in order to extensive cultivation and the standardization production of core fragrant plant, how research shortens core fragrant plant seed germinating time, raising percentage of seedgermination becomes this area technical problem urgently to be resolved hurrily.
Summary of the invention
The object of the present invention is to provide a kind of method that can promote core fragrant plant seed germination.
Technical solution of the present invention:
Core fragrant plant seed after immersion through destroying exosper process, is then carried out routine disinfection inoculation by the present invention, constant temperature culture makes seed germination.
Further: core fragrant plant seed is for long avette, and exosper yellowish-brown, surface is more smooth and have gloss, and quality is softer, and its thousand kernel weight is 865-885mg.
Further: the exosper of core fragrant plant seed is removed or at least cracks, but secundine will be kept complete not impaired.
The temperature of constant temperature culture of the present invention is 25 DEG C, and humidity is 60.
Further: core fragrant plant seed exosper destroys process and adopts mechanical failure or chemical reagent to soak, and mechanical failure adopts machine to roll or sand friction.
Because of the difference of habitat and kind, the Main Factors affecting seed germination is not identical yet, and find through on-the-spot investigation, the dominant factor affecting seed germination is temperature, humidity, pericarp thickness, kernel maturing.Find through Primary Study, in the self-reproduction of core fragrant plant seed field, pericarp thickness is the key factor of restriction core fragrant plant seed germination.Therefore the present invention does core fragrant plant seed and destroys exosper process.Find out the suitableeest method of core fragrant plant seed germination, provide a kind of germination rate high, germinating time is short, core fragrant plant seed germination method simple to operate, is intended to for efficiently and effectively protection and sustainable utilization core fragrant plant resource provide necessary basic data.
The good effect adopting the method for promotion core fragrant plant seed germination of the present invention to have is:
(1) the effective time shortening core fragrant plant seed germination, solve that its germination is weak, the low problem of germination rate;
(2) the present invention also have simple to operate, cost is low, germination rate is high, be easy to promote feature, to efficiently and effectively protection and sustainable utilization large-scale planting core fragrant plant resource have very high using value.
Embodiment
The experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent, if no special instructions, all can reach from commercial channels.
Embodiment 1,
core fragrant plant seed: picking up from Baotou periphery for planting experimentally son in the 6-7 month in 2014, being placed in paper envelopes, preserving under room temperature.
seed is selected: core fragrant plant seed is for long avette, and exosper yellowish-brown, surface is more smooth and have gloss, and quality is softer.Its thousand kernel weight is 874.500 ± 4.186mg.The full core fragrant plant seed that we select seed coat complete is as experiment material.
3. seed pre-treatment: be soaked in water the seed selected 3-4 hour, after taking out, covering of a seed keeps moistening, is divided into experimental group and control group.
4. exosper process is destroyed: adopt mechanical failure (machine rolls or sand friction) or chemical reagent process (reagent immersion) to be removed by the exosper of experimental group seed, but secundine will be kept complete not impaired.Control group seed does not do this process.
5. sterilization and inoculation: before inoculation, Seed sterilization sterilization be carried out, the core fragrant plant seed of experimental group and control group is placed in the triangular flask after sterilizing respectively, use aseptic water washing seed.Add 20% hypochlorite disinfectant 7 ~ 8 min, discard clorox, then add 20% hypochlorite disinfectant 7 ~ 8 min.After sterilization, discard clorox, with rinsed with sterile water seed 3 times, each 5 min.
6. the investigation of seed germination germination rate: spread one deck absorbent cotton in the culture dish that diameter is 9 cm, upper cover one deck filter paper, allow absorbent cotton and filter paper all suction moisture (sterile water), above each group seed is sprinkling upon on filter paper uniformly, seed is connected together as far as possible but does not suffer.Lid layer filter paper again on seed.50, the every ware of each process, repeats 3 times, is placed in 25 DEG C of climatic cabinates and cultivates, and keeps germinating bed moistening.Every day observes, and every 24 h record 1 time.Growing as seed length one half germination standard with plumule, 30 days is one-period, and that adds up seed starts the number of days that germinates, germination rate, germination vigor and germination index.
Germination rate=(seed sprouting sum/for planting experimentally subnumber) × 100%; Germination vigor=(subnumber is planted experimentally in the normal germination grain number/confession in germination vigor number of days) × 100%; Germination index (GI)=∑ (corresponding with Dt every day chitting piece number and germination number of days Gt/ germination number of days Dt)
7.
measurement result:through an experimental period (30 days), the seed of experimental group and control group all has sprouting in various degree, and sprouting appearred in experimental group seed from the 4th day, and sprouting appears in control group seed from the 7th talent.In the experimental period, the germination rate of experimental group seed is 93.63%, apparently higher than 43.33% of control group.
Core fragrant plant seed germination situation statistics (x=
± s, (n=3))
Note: * represents compared with control group, and P=0.05 level has pole significant difference.
Experiment conclusion:
(1) method for treating seeds that the present invention is used significantly can shift to an earlier date the sprouting of core fragrant plant seed;
(2) germination rate of the core fragrant plant seed after going exosper process is apparently higher than the core fragrant plant percentage of seedgermination do not processed and germination vigor and germination index.
Claims (6)
1. promote a method for core fragrant plant seed germination, it is characterized in that, core fragrant plant seed, through destroying exosper process, then carries out routine disinfection, inoculation, constant temperature culture makes seed germination.
2. a kind of method promoting core fragrant plant seed germination according to claim 1, is characterized in that, core fragrant plant seed is for long avette, and exosper yellowish-brown, surface is more smooth and have gloss, and quality is softer, and its thousand kernel weight is 865-885mg.
3. a kind of method promoting core fragrant plant seed germination according to claim 1, is characterized in that, the exosper of core fragrant plant seed is removed or at least cracked, but secundine will be kept complete not impaired.
4. a kind of method promoting core fragrant plant seed germination according to claim 1, is characterized in that, the temperature of constant temperature culture is 25 DEG C, and humidity is 60.
5. a kind of method promoting core fragrant plant seed germination according to claim 1, is characterized in that, core fragrant plant seed exosper destroys process and adopts mechanical failure or chemical reagent to soak, and mechanical failure adopts machine to roll or sand friction.
6. a kind of method promoting core fragrant plant seed germination according to claim 1, it is characterized in that, before inoculation, carry out Seed sterilization sterilization, adding mass concentration is 20% hypochlorite disinfectant 7 ~ 8 min, discard clorox, adding mass concentration is again 20% hypochlorite disinfectant 7 ~ 8 min, after sterilization, discards clorox, with water rinse seed 3 times, each 5 min.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410844940.9A CN104472062B (en) | 2014-12-31 | 2014-12-31 | A kind of method promoting core fragrant plant seed germination |
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| CN201410844940.9A CN104472062B (en) | 2014-12-31 | 2014-12-31 | A kind of method promoting core fragrant plant seed germination |
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| CN104472062A true CN104472062A (en) | 2015-04-01 |
| CN104472062B CN104472062B (en) | 2016-11-23 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145371A (en) * | 2015-10-12 | 2015-12-16 | 广西壮族自治区药用植物园 | Tissue culture breeding method for cymbaria dahurica |
| CN105165623A (en) * | 2015-10-12 | 2015-12-23 | 广西壮族自治区药用植物园 | Cymbaria mongolica maxim tissue culture breeding method |
| CN106489643A (en) * | 2016-10-19 | 2017-03-15 | 莱州市宏顺梅花种植科技有限公司 | A kind of Prunus mume var.bungo Makino breeding method |
| CN116870068A (en) * | 2023-08-07 | 2023-10-13 | 内蒙古科技大学包头医学院 | Application of shinyleaf yellowhorn leaf in preparation of medicine for preventing and treating hyperuricemia nephropathy |
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|---|---|---|---|---|
| CN101278650A (en) * | 2007-04-05 | 2008-10-08 | 上海市农业科学院园艺研究所 | Method for cultivating seedling and propagating tomato seeds |
| CN101451119A (en) * | 2008-12-26 | 2009-06-10 | 南京师范大学 | Phytolacca americana seed quick-speed germination method and its application in aseptic seedlings culture |
| CN101940158A (en) * | 2009-07-09 | 2011-01-12 | 正安县绿野科技产业有限责任公司 | Method for rapidly propagating plantlets by utilizing bletilla striata seeds |
| CN102487627A (en) * | 2011-12-09 | 2012-06-13 | 四川农业大学 | Method for effectively breaking dormancy of Paris polyphylla seeds |
-
2014
- 2014-12-31 CN CN201410844940.9A patent/CN104472062B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101278650A (en) * | 2007-04-05 | 2008-10-08 | 上海市农业科学院园艺研究所 | Method for cultivating seedling and propagating tomato seeds |
| CN101451119A (en) * | 2008-12-26 | 2009-06-10 | 南京师范大学 | Phytolacca americana seed quick-speed germination method and its application in aseptic seedlings culture |
| CN101940158A (en) * | 2009-07-09 | 2011-01-12 | 正安县绿野科技产业有限责任公司 | Method for rapidly propagating plantlets by utilizing bletilla striata seeds |
| CN102487627A (en) * | 2011-12-09 | 2012-06-13 | 四川农业大学 | Method for effectively breaking dormancy of Paris polyphylla seeds |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145371A (en) * | 2015-10-12 | 2015-12-16 | 广西壮族自治区药用植物园 | Tissue culture breeding method for cymbaria dahurica |
| CN105165623A (en) * | 2015-10-12 | 2015-12-23 | 广西壮族自治区药用植物园 | Cymbaria mongolica maxim tissue culture breeding method |
| CN105165623B (en) * | 2015-10-12 | 2017-04-26 | 广西壮族自治区药用植物园 | Cymbaria mongolica maxim tissue culture breeding method |
| CN105145371B (en) * | 2015-10-12 | 2017-06-23 | 广西壮族自治区药用植物园 | A kind of Da Wuli cores fragrant plant tissue culture method for breeding |
| CN106489643A (en) * | 2016-10-19 | 2017-03-15 | 莱州市宏顺梅花种植科技有限公司 | A kind of Prunus mume var.bungo Makino breeding method |
| CN116870068A (en) * | 2023-08-07 | 2023-10-13 | 内蒙古科技大学包头医学院 | Application of shinyleaf yellowhorn leaf in preparation of medicine for preventing and treating hyperuricemia nephropathy |
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| CN104472062B (en) | 2016-11-23 |
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Granted publication date: 20161123 |