CN102845158A - Method for rapid release of Callicarpa nudiflora seed dormancy - Google Patents
Method for rapid release of Callicarpa nudiflora seed dormancy Download PDFInfo
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- CN102845158A CN102845158A CN2012103090212A CN201210309021A CN102845158A CN 102845158 A CN102845158 A CN 102845158A CN 2012103090212 A CN2012103090212 A CN 2012103090212A CN 201210309021 A CN201210309021 A CN 201210309021A CN 102845158 A CN102845158 A CN 102845158A
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Abstract
The invention relates to a method for rapid release of Callicarpa nudiflora seed dormancy. The method comprises the following steps: 1) impregnating Callicarpa nudiflora seeds for 12-24h with one or more selected from 5-20 wt% of a plant ash water solution, 100-300mg / L agibberellin (GA3) aqueous solution and 100-300mg / L alpha-naphthaleneacetic acid (NAA), and then washing the seedswith water; and step 2) germinating the impregnated seeds in the step 1). The method for rapid release of Callicarpa nudiflora seed dormancy provided by the invention can effectively relieve various factors hindering germination of the Callicarpa nudiflora seeds, including mechanical barrier formed by the seed coat, break Callicarpa nudiflora seed dormancy, and enhance seed germination rate to 95%. Compared with a traditional seedling raising way without treatment, the method has advantages of shortened breeding cycle, uniform seedlings, and simple operation and low cost, and can be widely used for breeding and cultivation of a Callicarpa nudiflora medicinal material.
Description
Technical field
The present invention relates to the germination field of Seeds of Medicinal Plants, specifically, the present invention relates to the callicarpa nudiflora seed dormant method of a kind of quick releasing.
Background technology
Callicarpa nudiflora (Callecarpa nudiflora Hook.et Arn) is the callicarpa nudiflora dry blade of Verenaceae Callicarpa plant, records in " Chinese pharmacopoeia version in 1977.Have the styptic powder stasis of blood, the effects such as anti-inflammation are the important source material that callicarpa nudiflora, dispersible tablet of naked flower beautyberry leaf, callicarpa nudiflora capsule, callicarpa nudiflora particle, LUOHUAZIZHU SHUAN etc. nearly more than 40 plant Chinese patent drugs.To the callicarpa nudiflora great demand as medical material, it is serious to cause wild resource to destroy, gather wild resource and can not satisfy need of production, carrying out standardization production is the effective way that solves the medicinal material shortage, and seedling breeding is the important component part that standardization is produced, because the callicarpa nudiflora fruit maturation cycle is longer, kernel maturing differs, add that seed is narrow and small, the kind skin is hard etc., cause the callicarpa nudiflora seedling breeding cycle longer, it is inconsistent to emerge, and has had a strong impact on callicarpa nudiflora standardized cultivation.
By the retrieval to domestic and foreign literature and patent, about callicarpa nudiflora seed sprouting and sapling multiplication retrieve 2 pieces of documents altogether, (the Huang Qiuyin such as Huang Qiuyin, blue ancestral plants, Pan Chunliu, Huang Hao. the research of callicarpa nudiflora Seed Germination factor. Agriculture of Anhui science, 2009 (25): 12006-7.) carry out the seed germination experiment of callicarpa nudiflora differing maturity, different substrates, different freshness.Found that, callicarpa nudiflora when the aobvious purple of fruit the seed sprouting best results, germination rate reaches as high as 73.0%; Seed do to be sprouted the matrix effect of can being germinateed preferably with river sand, and germination rate is 66.0%; Fresh seeds is sprouted effective than stored seeds, germination rate is up to 72.8%.(Nie's Yao such as Nie's Yao, Wu Yongzhong, Yu Baoping, Xiao Junping. callicarpa nudiflora seedling breeding and culture technique. modern horticultural 2010 (12): 23-4.) callicarpa nudiflora seedling breeding and field production technology are studied, wherein seedling breeding comprises that old branch cutting propagation and epicormic branch cuttage breed; The field production technology mainly comprises selection soil, plantation time and density, tree-like selection and field management and timely collecting.
Difference according to its processing method in the seed germination experiment can be divided into physical treatment and chemical treatment two classes, wherein physical treatment mainly comprises 3 kinds of mechanical treatments, Temperature Treatment, ultrasonic processing, chemical treatment can be divided into 5 classes such as acid treatment, alkaline treatment, oxidizer treatment, salt processing, HORMONE TREATMENT, can be divided into 3 classes again according to mechanism that seed dormancy is abolished: (1) softening kind of skin, improve Permeable characteristic between embryo and external environment etc., mainly contain Temperature Treatment, salt acid treatment, sodium bicarbonate is processed and hydrogen peroxide is processed 4 kinds of processing modes; (2) nutrition is processed, and mainly contains ash, calcium chloride processing, potassium nitrate processing, iron chloride processing and boric acid and processes 4 kinds of processing modes; (3) HORMONE TREATMENT mainly contains growth hormone processing, basic element of cell division processing and gibberellin and processes 3 double recipe formulas.Be the research of the callicarpa nudiflora Germination characteristics of comprehensive study, the present invention inquires into ash, growth hormone processing and gibberellin to the research of callicarpa nudiflora Germination Characteristics.
Summary of the invention
The purpose of this invention is to provide the callicarpa nudiflora seed dormant method of a kind of quick releasing.
In order to realize purpose of the present invention, the invention provides the callicarpa nudiflora seed dormant method of a kind of quick releasing, the method may further comprise the steps:
Step 1): callicarpa nudiflora seed is used respectively the ash aqueous solution of 5 % by weight ~ 20 % by weight, the gibberellin (GA of 100 ~ 300mg/L
3) one or more dippings 12 ~ 24h in α-naphthaleneacetic acid (NAA) aqueous solution of the aqueous solution and 100 ~ 300mg/L, then wash; And
Step 2): the callicarpa nudiflora seed after flooding in the described step 1) is germinateed.
Preferably, in described step 1), with the ash aqueous solution dipping 12 ~ 24h of callicarpa nudiflora seed with 5 % by weight ~ 20 % by weight.
Preferably, in described step 1), with the gibberellin (GA of callicarpa nudiflora seed with 100 ~ 300mg/L
3) aqueous solution dipping 12 ~ 24h.
Preferably, in described step 1), with α-naphthaleneacetic acid (NAA) the aqueous solution dipping 12 ~ 24h of callicarpa nudiflora seed with 100 ~ 300mg/L.
Preferably, in described step 1), with callicarpa nudiflora seed use respectively the ash aqueous solution dipping 12 ~ 24h of 5 % by weight ~ 20 % by weight, with the gibberellin (GA of 100 ~ 300mg/L
3) aqueous solution dipping 12 ~ 24h and flood 12 ~ 24h with α-naphthaleneacetic acid (NAA) aqueous solution of 100 ~ 300mg/L.
Herein, within the scope of the invention, term " difference " does not carry out any restriction to the order of operation.For example, callicarpa nudiflora seed is used respectively the ash aqueous solution dipping 12 ~ 24h of 5 % by weight ~ 20 % by weight, with the gibberellin (GA of 100 ~ 300mg/L
3) aqueous solution dipping 12 ~ 24h and with α-naphthaleneacetic acid (NAA) aqueous solution dipping 12 ~ 24h of 100 ~ 300mg/L, can at first use the ash aqueous solution processing of 5 % by weight ~ 20 % by weight, also can at first use the gibberellin (GA of 100 ~ 300mg/L
3) α-naphthaleneacetic acid (NAA) aqueous solution of the aqueous solution or 100 ~ 300mg/L processes.
As used in this article, term " is cleaned " and is referred to wash that chemical treatments is trace to the eluent.
As used in this article, term " dipping " refers to the state of seed under liquid level.
Preferably, in described step 2) in, callicarpa nudiflora seed behind the dipping in the described step 1) is changed in the illumination box of constant temperature or temperature match curing conditions, at vermiculite, wet sand, filter paper, sponge germinating bed or mix in the sand of 30 % by weight ~ 40 % by weight coconut palm chaffs and germinate.
More preferably, the callicarpa nudiflora seed behind the dipping in the described step 1) is changed in the illumination box of constant temperature or temperature match curing conditions, mix in the sand of 30 % by weight ~ 40 % by weight coconut palm chaffs and germinate.
Preferably, also be included in and carry out before the described step 1), carry out the selected step of separating with seed of fruit.
More preferably, in the selected step of described fruit, from healthy, pluck white fruit without the callicarpa nudiflora tree of damage by disease and insect and reach fruit more than 80%, then use 6 ~ 8 purpose sieve, can not be ripening fruits by the fruit of 6 ~ 8 eye mesh screens.
More preferably, in the step that described seed separates, with selected ripening fruits with after crossing the ratio mixing of quartz sand according to weight ratio 1:1.5 ~ 1:2 of 20 mesh sieves, repeatedly wash by rubbing with the hands, until seed is separated from fruit, then water is removed pericarp and fruit inclusion, after 20 mesh sieves, quartz sand is removed, namely got callicarpa nudiflora seed.
The method of the callicarpa nudiflora seed dormancy of quick releasing of the present invention, can efficient solution remove the various factors that hinders callicarpa nudiflora seed germination, comprise the formed mechanical obstacles of kind of skin, break callicarpa nudiflora seed dormancy, significantly improve percentage of seedgermination, germination rate can reach 95.0%, compares without the seedling raising manners of processing with tradition, have and shorten growing-seedling period, seedling neat and consistent, the advantage such as simple to operate, with low cost, can be widely used in seedling breeding and the cultivation of callicarpa nudiflora medicinal material.
Embodiment
Following examples are used for explanation the present invention, but are not used for limiting the scope of the invention.
Illustrate:
1. the germination test that adopts in the following example (except embodiment 5) is paper bed germination test method, specific as follows: diameter is the interior filter paper of placing of the culture dish of 9cm, water is drenched, unnecessary water is outwelled, take moisturizing not ponding as degree, choose 100 callicarpa nudiflora seeds and evenly put on the paper of culture dish, put into microcomputerized control illumination box (LRH-250-G11 after again culture dish being built; Guangdong Medical Apparatus and Instruments Factory produces), germinate according to the designed specified temp of each test example (constant temperature or alternating temperature) condition, all carry out illumination in the whole germination process; Detect 1 ~ 2 every day in the germination process, and suitably supply moisture, and callicarpa nudiflora seed is counted 1 time after beginning to germinate every day.Discovery has corrupt seed to sort out at any time, if mildew seed〉5% should in time change the filter paper in the culture dish, and put into again culture dish after seed cleaned with distilled water and continue to make its germination.
2. with reference to " international seed inspection procedure " (1985), the standard (to grow 1cm as germination) whether germinate as the evaluation seed " crop seeds inspection procedure " (1995) etc.
3. percentage of seedgermination refers to that germination test is final, and whole seed number of germinateing within the date of regulation account for for the percentage of planting experimentally son.Seed number * 100% is planted experimentally in chitting piece in germination rate (%)=fixed date/confession.The germination date in this test is 20 days from the germination paper bed being put into case.
4. this experiment attempts to break by several different methods the dormancy of callicarpa nudiflora seed, but only have ash, gibberellin and growth hormone can break the dormancy of callicarpa nudiflora seed, other method example hydrochloric acid processing, hydrogen peroxide processing etc. all can not be removed callicarpa nudiflora seed dormancy.
Experiment material
Used callicarpa nudiflora (C.nudiflora) seed of this experiment is the ripe callicarpa nudiflora fruit of white of gathering in cities and counties such as Danzhou, Hainan Province, the Wuzhi Mountain, white sand, Haikous year January in December, 2011 ~ 2012, with 6 ~ 8 purpose sieve, can not be for subsequent use by the fruit of 6 ~ 8 eye mesh screens.With selected ripening fruits with after crossing the ratio mixing of quartz sand according to weight ratio 1:2 of 20 mesh sieves, repeatedly wash by rubbing with the hands, until seed is separated from fruit, then water is removed pericarp and fruit inclusion, after 20 mesh sieves, quartz sand is removed, is namely got callicarpa nudiflora seed, with it as experiment material.
Embodiment 1
Experimental technique
Callicarpa nudiflora seed is divided into two groups, and one group with 0.1% hydrochloric acid (i.e. 0.1 volume % aqueous hydrochloric acid solution) dipping certain hour, one group of blank.Then with 1 % by weight aqueous sodium hypochlorite solution sterilization 15 minutes, rinsed with sterile water 5 times is to pH=7.Parallel four groups of each concentration, 100 every group place 30 ℃ of constant incubator full exposures to cultivate, add up germination rate every day.
The acid treatment of table 10.1% salt is on the impact of callicarpa nudiflora seed germination
Experimental result
As shown in table 1, when callicarpa nudiflora seed during without 0.1% salt acid treatment, about the 10th day, namely begin to sprout, but germination rate is lower, germination rate is lower than 5.0%; Begin to have one to sprout the phase of concentrating when about 20d, per day germination rate can be up to 12.0%; Reach peak value at 30d, the callicarpa nudiflora seed germination about 83.0% is arranged.After callicarpa nudiflora seed is with 0.1% hydrochloric acid, 1 ~ 30min, its sprout to concentrate the phase with without the acid-treated callicarpa nudiflora seed of 0.1% salt same period, about 20d, per day germination rate reaches 12.0% ± 2.0%; Reach peak value at 30d, be 81.0% ± 4.0% through the acid-treated callicarpa nudiflora seed germination rate of 0.1% salt, with without the acid-treated percentage of seedgermination of 0.1% salt without significant difference, so draw to draw a conclusion from this research: with the callicarpa nudiflora seed of 0.1% salt acid treatment, callicarpa nudiflora Seed germination time and germination rate are not all made significant difference.
Embodiment 2
Experimental technique
Callicarpa nudiflora seed is divided into two groups, one group of hydrogen peroxide dipping 2h with variable concentrations (W/W), one group of blank.Then with 1 % by weight aqueous sodium hypochlorite solution sterilization 15 minutes, rinsed with sterile water 5 times is to pH=7.Parallel four groups of each concentration, 100 every group placed 30 ℃ of constant incubator full exposures to cultivate, every statistics germination rate on the 5th.
Table 2 hydrogen peroxide is processed the impact on callicarpa nudiflora seed germination
Experimental result
As shown in table 2, when callicarpa nudiflora seed is processed without hydrogen peroxide, about the 10th day, namely begin to sprout, but germination rate is lower, germination rate is lower than 5.0%; Begin to have one to sprout the phase of concentrating when about 20d, per day germination rate can be up to 12.0%; Reach peak value at 30d, the callicarpa nudiflora seed germination about 83.0% is arranged.After with 0.05% ~ 0.3% hydrogen peroxide 2h, it sprouts the phase of concentrating and the callicarpa nudiflora seed same period of processing without hydrogen peroxide, and about 20d, per day germination rate reaches 12.0% ± 2.0%; Reach peak value at 30d, the callicarpa nudiflora seed germination rate of processing through hydrogen peroxide is 81.0% ± 3.0%, with the percentage of seedgermination of processing without hydrogen peroxide without significant difference, so draw to draw a conclusion from this research: process callicarpa nudiflora seed with hydrogen peroxide, callicarpa nudiflora Seed germination time and germination rate are not all made significant difference.
Embodiment 3
Experimental technique
Callicarpa nudiflora seed is divided into two groups, one group of ash aqueous solution dipping 24h with variable concentrations, one group of blank.Then with 1 % by weight aqueous sodium hypochlorite solution sterilization 15 minutes, rinsed with sterile water 5 times is to pH=7.Parallel four groups of each concentration, 100 every group placed 30 ℃ of constant incubator full exposures to cultivate, every statistics germination rate on the 5th.
Table 3 ash is processed the impact on callicarpa nudiflora seed germination
Experimental result
As shown in table 3, when callicarpa nudiflora seed is processed without the ash aqueous solution, about the 10th day, namely begin to sprout, but germination rate is lower, germination rate is lower than 5.0%; Begin to have one to sprout the phase of concentrating when about 20d, per day germination rate can be up to 12.0%; Reach peak value at 30d, the callicarpa nudiflora seed germination about 93.0% is arranged.After with 5% ~ 20% ash aqueous solution dipping 24h, it sprouts the phase of concentrating and the callicarpa nudiflora seed same period of processing without the ash aqueous solution, about 20d, but its per day germination rate is increased to 16.0% ± 1.0%, and reach peak value at 20d, the callicarpa nudiflora seed germination rate of processing through the ash aqueous solution is 95.0% ± 2.0%, there were significant differences with the percentage of seedgermination of processing without the ash aqueous solution, so draw to draw a conclusion from this research: process callicarpa nudiflora seed with the ash aqueous solution, all there are appreciable impact callicarpa nudiflora Seed germination time and germination rate.
Embodiment 4
Experimental technique
Callicarpa nudiflora seed is divided into two groups, one group of gibberellin aqueous solution dipping 24h with variable concentrations, one group of blank.Then with 1 % by weight aqueous sodium hypochlorite solution sterilization 15 minutes, rinsed with sterile water 5 times is to pH=7.Parallel four groups of each concentration, 100 every group placed 30 ℃ of constant incubator full exposures to cultivate, every statistics germination rate on the 5th.
Table 4 gibberellin is processed the impact on callicarpa nudiflora seed germination
Experimental result
As shown in table 4, when callicarpa nudiflora seed is processed without the gibberellin aqueous solution, about the 10th day, namely begin to sprout, but germination rate is lower, germination rate is lower than 5.0%; Begin to have one to sprout the phase of concentrating when about 20d, per day germination rate can be up to 12.0%; Reach peak value at 30d, the callicarpa nudiflora seed germination about 83.0% is arranged.After with 100 ~ 300mg/L gibberellin aqueous solution 24h, it just begins to sprout in 5d, and at 10d, callicarpa nudiflora Seed germination rate can reach about 10%, and 15d is that callicarpa nudiflora seed germination is concentrated the phase, and per day germination rate reaches 12.0% ± 2.0%; Reach peak value at 20d, the callicarpa nudiflora seed germination rate of processing through the gibberellin aqueous solution is 81.0% ± 3.0%, so draw to draw a conclusion from this research: process callicarpa nudiflora seed with the gibberellin aqueous solution, can shorten the callicarpa nudiflora Seed germination time, but callicarpa nudiflora percentage of seedgermination is not made significant difference.
Embodiment 5
Experimental technique
Callicarpa nudiflora seed is divided into two groups, one group of α-naphthaleneacetic acid (NAA) aqueous solution dipping 24h with variable concentrations, one group of blank.Then with 1 % by weight aqueous sodium hypochlorite solution sterilization 15 minutes, rinsed with sterile water 5 times is to pH=7.Parallel four groups of each concentration, 100 every group placed 30 ℃ of constant incubator full exposures to cultivate, every statistics germination rate on the 5th.
Table 5NAA processes the impact on callicarpa nudiflora seed germination
Experimental result
As shown in table 5, when callicarpa nudiflora seed is processed without the NAA aqueous solution, about the 10th day, namely begin to sprout, but germination rate is lower, germination rate is lower than 5.0%; Begin to have one to sprout the phase of concentrating when about 20d, per day germination rate can be up to 12.0%; Reach peak value at 30d, the callicarpa nudiflora seed germination about 83.0% is arranged.After processing 24h with 100 ~ 300mg/L NAA aqueous solution, it just begins to sprout in 5d, and at 10d, callicarpa nudiflora Seed germination rate can reach about 10%, and 15d is that callicarpa nudiflora seed germination is concentrated the phase, and per day germination rate reaches 12.0% ± 2.0%; Reach peak value at 20d, the callicarpa nudiflora seed germination rate of processing through the NAA aqueous solution is 85.0% ± 3.0%, so draw to draw a conclusion from this research: process callicarpa nudiflora seed with the NAA aqueous solution, not only can shorten the callicarpa nudiflora Seed germination time, can also improve to a certain extent the germination rate of callicarpa nudiflora seed.
Embodiment 6
Experimental technique
Callicarpa nudiflora seed is divided into 14 groups, test with reference to table 6 is processed, first callicarpa nudiflora seed is processed 24h with the ash aqueous solution of 5 % by weight ~ 15 % by weight concentration, use again the gibberellin aqueous solution of 100 ~ 300mg/L to process 24h, use at last α-naphthaleneacetic acid (NAA) aqueous solution of 100 ~ 300mg/L to process 24h, then with 1 % by weight aqueous sodium hypochlorite solution sterilization 15 minutes, rinsed with sterile water 5 times is to pH=7.Parallel 3 groups of each concentration, 100 every group placed 30 ℃ of constant incubator full exposures to cultivate, every statistics germination rate on the 5th.
The callicarpa nudiflora seed of table 6 " ash, gibberellin and NAA " three factors " 3414 " Combined Processing
Table 7 " ash, gibberellin and NAA " three factor Combined Processing are on the impact of callicarpa nudiflora germination rate
Experimental result
As shown in table 7, studied ash, gibberellin and growth hormone α-naphthaleneacetic acid (NAA) the various combination otherness to callicarpa nudiflora seed germination, the result shows: 1. callicarpa nudiflora seed germination and ash, gibberellin and α-naphthaleneacetic acid (NAA) are flat closely related for trying the water, and are in the medium level (B of design when gibberellin and α-naphthaleneacetic acid (NAA) consumption
2C
2) time, increasing the ash consumption and can significantly improve callicarpa nudiflora percentage of seedgermination, and shorten dormancy time, callicarpa nudiflora percentage of seedgermination highest point reason is A
3B
2C
2, high germination rate is 96.0% ± 1.0%; 2. be medium design amount of application (A at ash and growth hormone
2C
2) time, 100mg/L can significantly improve callicarpa nudiflora percentage of seedgermination, and shortens dormancy time, it just can reach 34.0% ± 2.0% at 10d, it can reach 93.0% ± 2.0% germination rate at 15d, but when further increasing gibberellin concentration, germination rate has the trend of reduction; 3. working as ash and gibberellin is medium design amount of application (B
2C
2) time, 100mg/L NAA can significantly improve callicarpa nudiflora percentage of seedgermination, and shortens the callicarpa nudiflora seed dormancy time, but when further improving NAA concentration, its facilitation becomes not obvious.
In ash, gibberellin and NAA Combined Processing, all meet processing and have all significantly promoted callicarpa nudiflora percentage of seedgermination, and have shortened callicarpa nudiflora seed germinating time, wherein with A
3B
2C
2, A
2B
1C
2And A
2B
1C
1The effect of 3 processing is the most remarkable, and it can be increased to 96.0% ± 1.0% with callicarpa nudiflora percentage of seedgermination; A
2B
1C
2And A
2B
2C
12 processing are to shortening the callicarpa nudiflora seed dormancy time, improving germination vigor and have remarkable result, and it just can reach 34.0% ~ 37.0% germination rate at 10d, can reach 91.0% ~ 93.0% germination rate at 15d.
Embodiment 7
Experimental technique
Callicarpa nudiflora seed is divided into 4 groups, the 1st group, directly callicarpa nudiflora seed directly is seeded in the cultivation matrix that is mixed with farmyard manure, 30 % by weight ~ 40 % by weight coconut palm chaffs, 15 % by weight ~ 20 % by weight vermiculites or fine sand and 30 % by weight ~ 40 % by weight black earth that 15 % by weight ~ 20 % by weight are become thoroughly decomposed; The 2nd group, process respectively 24h with being mixed with the 20 % by weight ash aqueous solution, the 200mg/L gibberellin aqueous solution and the 200mg/L NAA aqueous solution, then flowing water flushing 30min is seeded in the cultivation matrix that is mixed with farmyard manure, 30 % by weight ~ 40 % by weight coconut palm chaffs, 15 % by weight ~ 20 % by weight vermiculites or fine sand and 30 % by weight ~ 40 % by weight black earth that 15 % by weight ~ 20 % by weight are become thoroughly decomposed; The 3rd group, process respectively 24h with being mixed with the 10 % by weight ash aqueous solution, the 100mg/L gibberellin aqueous solution and the 200mg/L NAA aqueous solution, then flowing water flushing 30min is seeded in the cultivation matrix that is mixed with farmyard manure, 30 % by weight ~ 40 % by weight coconut palm chaffs, 15 % by weight ~ 20 % by weight vermiculites or fine sand and 30 % by weight ~ 40 % by weight black earth that 15 % by weight ~ 20 % by weight are become thoroughly decomposed; The 4th group, process respectively 24h with being mixed with the 10 % by weight ash aqueous solution, the 200mg/L gibberellin aqueous solution and the 100mg/L NAA aqueous solution, then flowing water flushing 30min is seeded in the cultivation matrix that is mixed with farmyard manure, 30 % by weight ~ 40 % by weight coconut palm chaffs, 15 % by weight ~ 20 % by weight vermiculites or fine sand and 30 % by weight ~ 40 % by weight black earth that 15 % by weight ~ 20 % by weight are become thoroughly decomposed.Each processes parallel 3 groups, and 100 every group, every statistics germination rate on the 10th.
Table 8 ash, gibberellin and NAA Combined Processing are on the impact of callicarpa nudiflora germination rate
Experimental result
As shown in table 8, to process in 1 in cultivation, it has callicarpa nudiflora seed germination about 30 days, in 70d, percentage of seedgermination can reach 83.0% ± 3.0%, in other 3 kinds of processing, at 20d after planting, namely begin to have 3.0% ~ 5.0% callicarpa nudiflora seed to begin to sprout, at 50d after planting, its germination rate can reach 87.0% ~ 92.0%, 50d after planting, its germination rate reaches the highest, and it can reach 92.0% ~ 94.0%.Compare with not treated callicarpa nudiflora seed, through processing method of the present invention, can improve callicarpa nudiflora percentage of seedgermination 9% ~ 13%, shorten seed germinating time 20 ~ 30d.
Claims (10)
1. remove fast callicarpa nudiflora seed dormant method for one kind, the method may further comprise the steps:
Step 1): callicarpa nudiflora seed is used respectively the ash aqueous solution of 5 % by weight ~ 20 % by weight, the gibberellin (GA of 100 ~ 300mg/L
3) one or more dippings 12 ~ 24h in α-naphthaleneacetic acid (NAA) aqueous solution of the aqueous solution and 100 ~ 300mg/L, then wash; And
Step 2): the callicarpa nudiflora seed after flooding in the described step 1) is germinateed.
2. the callicarpa nudiflora seed dormant method of quick releasing according to claim 1 is characterized in that: in described step 1), with the ash aqueous solution dipping 12 ~ 24h of callicarpa nudiflora seed with 5 % by weight ~ 20 % by weight.
3. the callicarpa nudiflora seed dormant method of quick releasing according to claim 1 is characterized in that: in described step 1), with the gibberellin (GA of callicarpa nudiflora seed with 100 ~ 300mg/L
3) aqueous solution dipping 12 ~ 24h.
4. the callicarpa nudiflora seed dormant method of quick releasing according to claim 1 is characterized in that: in described step 1), with α-naphthaleneacetic acid (NAA) the aqueous solution dipping 12 ~ 24h of callicarpa nudiflora seed with 100 ~ 300mg/L.
5. the callicarpa nudiflora seed dormant method of quick releasing according to claim 1, it is characterized in that: in described step 1), with callicarpa nudiflora seed use respectively the ash aqueous solution dipping 12 ~ 24h of 5 % by weight ~ 20 % by weight, with the gibberellin (GA of 100 ~ 300mg/L
3) aqueous solution dipping 12 ~ 24h and flood 12 ~ 24h with α-naphthaleneacetic acid (NAA) aqueous solution of 100 ~ 300mg/L.
6. the callicarpa nudiflora seed dormant method of each described quick releasing in 5 according to claim 1, it is characterized in that: in described step 2) in, callicarpa nudiflora seed behind the dipping in the described step 1) is changed in the illumination box of constant temperature or temperature match curing conditions, at vermiculite, wet sand, filter paper, sponge germinating bed or mix in the sand of 30 % by weight ~ 40 % by weight coconut palm chaffs and germinate.
7. the callicarpa nudiflora seed dormant method of quick releasing according to claim 6, it is characterized in that: the callicarpa nudiflora seed behind the dipping in the described step 1) is changed in the illumination box of constant temperature or temperature match curing conditions, mix in the sand of 30 % by weight ~ 40 % by weight coconut palm chaffs and germinate.
8. the callicarpa nudiflora seed dormant method of quick releasing according to claim 1 is characterized in that: also be included in and carry out before the described step 1), carry out the selected step of separating with seed of fruit.
9. the callicarpa nudiflora seed dormant method of quick releasing according to claim 8, it is characterized in that: in the selected step of described fruit, from healthy, pluck white fruit without the callicarpa nudiflora tree of damage by disease and insect and reach fruit more than 80%, then use 6 ~ 8 purpose sieve, can not be ripening fruits by the fruit of 6 ~ 8 eye mesh screens.
10. according to claim 8 or the callicarpa nudiflora seed dormant method of 9 described quick releasings, it is characterized in that: in the step that described seed separates, with selected ripening fruits with after crossing the ratio mixing of quartz sand according to weight ratio 1:1.5 ~ 1:2 of 20 mesh sieves, repeatedly wash by rubbing with the hands, until seed is separated from fruit, then water is removed pericarp and fruit inclusion, after 20 mesh sieves, quartz sand is removed, namely got callicarpa nudiflora seed.
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| CN103039250A (en) * | 2013-01-09 | 2013-04-17 | 吴嵩滨 | Method for planting callicarpa nudiflora seedlings |
| CN104054427A (en) * | 2014-06-06 | 2014-09-24 | 中国热带农业科学院热带作物品种资源研究所 | Method for enhancing germination rate of fimbristylis dichotoma seed |
| CN105961002A (en) * | 2016-05-25 | 2016-09-28 | 郎溪县天香油用牡丹种植专业合作社 | Seedling growing method for oil peonies |
| CN108496796A (en) * | 2018-03-09 | 2018-09-07 | 浙江省亚热带作物研究所 | A kind of Callicarpa bodinieri Levl. aseptic seeding rapid propagation method |
| CN109005744A (en) * | 2018-09-15 | 2018-12-18 | 烟台市林业科学研究所 | A method of Callicarpa bodinieri Levl. seed germination rate is improved using gibberellin |
| CN114946497A (en) * | 2022-04-27 | 2022-08-30 | 江西普正制药股份有限公司 | Seedling raising method for improving germination rate and regularity of callicarpa nudiflora |
| CN116171781A (en) * | 2022-09-06 | 2023-05-30 | 中国长江三峡集团有限公司 | Container seedling raising method for efficiently breeding photinia fraseri |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103039250A (en) * | 2013-01-09 | 2013-04-17 | 吴嵩滨 | Method for planting callicarpa nudiflora seedlings |
| CN104054427A (en) * | 2014-06-06 | 2014-09-24 | 中国热带农业科学院热带作物品种资源研究所 | Method for enhancing germination rate of fimbristylis dichotoma seed |
| CN104054427B (en) * | 2014-06-06 | 2016-07-06 | 中国热带农业科学院热带作物品种资源研究所 | A kind of method improving Herba Fimbristylis dichotomae percentage of seedgermination |
| CN105961002A (en) * | 2016-05-25 | 2016-09-28 | 郎溪县天香油用牡丹种植专业合作社 | Seedling growing method for oil peonies |
| CN108496796A (en) * | 2018-03-09 | 2018-09-07 | 浙江省亚热带作物研究所 | A kind of Callicarpa bodinieri Levl. aseptic seeding rapid propagation method |
| CN109005744A (en) * | 2018-09-15 | 2018-12-18 | 烟台市林业科学研究所 | A method of Callicarpa bodinieri Levl. seed germination rate is improved using gibberellin |
| CN114946497A (en) * | 2022-04-27 | 2022-08-30 | 江西普正制药股份有限公司 | Seedling raising method for improving germination rate and regularity of callicarpa nudiflora |
| CN116171781A (en) * | 2022-09-06 | 2023-05-30 | 中国长江三峡集团有限公司 | Container seedling raising method for efficiently breeding photinia fraseri |
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