CN115989784A - Method for improving germination rate and seedling survival rate of lonicera macranthoides seeds - Google Patents
Method for improving germination rate and seedling survival rate of lonicera macranthoides seeds Download PDFInfo
- Publication number
- CN115989784A CN115989784A CN202310112137.5A CN202310112137A CN115989784A CN 115989784 A CN115989784 A CN 115989784A CN 202310112137 A CN202310112137 A CN 202310112137A CN 115989784 A CN115989784 A CN 115989784A
- Authority
- CN
- China
- Prior art keywords
- seeds
- germination
- lonicera
- matrix
- hours
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000035784 germination Effects 0.000 title claims abstract description 77
- 238000000034 method Methods 0.000 title claims abstract description 58
- 241001170076 Lonicera macranthoides Species 0.000 title claims abstract description 35
- 230000004083 survival effect Effects 0.000 title claims abstract description 14
- 239000011159 matrix material Substances 0.000 claims abstract description 29
- 229920000742 Cotton Polymers 0.000 claims abstract description 15
- 241000245240 Lonicera Species 0.000 claims abstract description 12
- 238000002791 soaking Methods 0.000 claims abstract description 12
- 239000003415 peat Substances 0.000 claims abstract description 10
- 239000010451 perlite Substances 0.000 claims abstract description 10
- 235000019362 perlite Nutrition 0.000 claims abstract description 10
- 239000010455 vermiculite Substances 0.000 claims abstract description 10
- 235000019354 vermiculite Nutrition 0.000 claims abstract description 10
- 229910052902 vermiculite Inorganic materials 0.000 claims abstract description 10
- 239000003337 fertilizer Substances 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 238000011282 treatment Methods 0.000 claims description 54
- 238000005286 illumination Methods 0.000 claims description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 36
- 230000007613 environmental effect Effects 0.000 claims description 18
- 239000012153 distilled water Substances 0.000 claims description 17
- 239000010410 layer Substances 0.000 claims description 14
- 239000002245 particle Substances 0.000 claims description 14
- 235000013399 edible fruits Nutrition 0.000 claims description 9
- 239000000835 fiber Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 241000628997 Flos Species 0.000 claims description 5
- 229920006395 saturated elastomer Polymers 0.000 claims description 5
- 239000000758 substrate Substances 0.000 claims description 5
- 239000002344 surface layer Substances 0.000 claims description 4
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 3
- 241001570521 Lonicera periclymenum Species 0.000 claims description 3
- 238000009825 accumulation Methods 0.000 claims description 3
- 238000007605 air drying Methods 0.000 claims description 3
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 3
- 239000006013 carbendazim Substances 0.000 claims description 3
- 239000012286 potassium permanganate Substances 0.000 claims description 3
- 238000007781 pre-processing Methods 0.000 claims description 3
- -1 salt ions Chemical class 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- 238000009395 breeding Methods 0.000 claims description 2
- 230000001488 breeding effect Effects 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 230000001502 supplementing effect Effects 0.000 claims description 2
- 240000002734 Lonicera caerulea Species 0.000 abstract description 2
- 235000001387 Lonicera caerulea Nutrition 0.000 abstract description 2
- 230000007226 seed germination Effects 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 230000004720 fertilization Effects 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 241000208828 Caprifoliaceae Species 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 244000167230 Lonicera japonica Species 0.000 description 1
- 235000017617 Lonicera japonica Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for improving germination rate and seedling survival rate of lonicera macranthoides seeds. The method comprises the following steps: the lonicera caerulea seeds are placed between sterile cotton germination accelerating papers at the upper part and the lower part respectively, and are placed on the sterile cotton germination accelerating papers after seed soaking; accelerating germination; mixing perlite, vermiculite and peat into a matrix; when the seeds of lonicera macranthoides sprout, only radicle of the lonicera macranthoides are embedded into a matrix; placing the planted seedlings in a climatic chamber; when the cotyledons of lonicera flos lonicera seedlings begin to wither, applying N on the surface matrix: p: k slow release mixed fertilizer. The invention obviously improves the seed germination rate, the seedling survival rate and the seedling quality, and greatly increases the population quantity.
Description
Technical Field
The invention relates to the technical field of plant breeding, in particular to a method for improving germination rate and seedling survival rate of lonicera macranthoides seeds.
Background
Lonicera lilacina (Lonicera obata) is a Lonicera japonica (Lonicera) deciduous shrub of Caprifoliaceae (Caprifoliaceae), and is mainly distributed on a hillside with an altitude of 1200-1400 m. The lonicera macranthoides has unique taxonomic status in the lonicera plant group, so that important scientific value can be provided for the lonicera plant origin and phylogenetic development.
The species has very few field distribution, and is important for protecting wild plants.
For many years, scientific researchers try to artificially propagate lonicera macranthoides in various modes such as cutting, tissue culture, seed sowing and the like, but the effect is not ideal. Thus, the development of germination accelerating and seedling cultivating technology for lonicera caerulea seeds is helpful for increasing the number of individuals in the extremely small population and maintaining the genetic diversity of the species.
Therefore, how to provide a method for improving the germination rate and the survival rate of seedlings of lonicera macranthoides seeds is a problem to be solved by the person skilled in the art.
Disclosure of Invention
In view of this, the present invention provides a method for improving the germination rate and seedling survival rate of lonicera macranthoides seeds.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a method for improving germination rate and seedling survival rate of lonicera macranthoides seeds comprises the following steps:
(1) Collecting mature lonicera macranthoides fruits, and preprocessing to obtain seeds;
(2) Placing the seeds obtained in the step (1) between 3 layers of sterile cotton germination accelerating paper on the upper and lower sides, dripping distilled water to saturate the germination accelerating paper with water, then placing the seeds into a culture dish, soaking the seeds in the environment of 25 ℃ for 24 hours, taking the seeds out of the germination accelerating paper, placing the seeds on 2 layers of sterile cotton germination accelerating paper laid on the culture dish, dripping distilled water until the germination accelerating paper is saturated with water, and no obvious water accumulation exists in the culture dish; keeping the culture dish closed by 90%, accelerating germination, and periodically supplementing water;
(3) Perlite, vermiculite and peat are mixed according to the volume ratio of 1:1:2.5, mixing the materials into a matrix, sterilizing, filling the matrix into a container, pouring enough water, and digging a hole with the diameter of 2-3 mm and the depth of 1-1.5 cm on the surface of the matrix in the middle of the container for standby;
(4) After the seeds of lonicera macranthoides sprout, burying radicle of lonicera macranthoides into the hole in the step (3);
(5) Placing planted seedlings in a climatic chamber under the following environmental conditions: day 28 ℃,12 hours, and illumination intensity 300 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 60-70%;
(6) When the cotyledons of the lonicera flos lonicerae seedlings begin to wither, the substrate is on the surface layer according to 0.026g/cm 2 N is applied in the ratio of: p: the mass ratio of K is 1:1: 1.
Preferably: step (1) pretreatment: naturally air-drying the fruit in the environment without direct sunlight, and removing pulp after the fruit is dried and shrunk.
Preferably: step (2) environment: placing in a seed incubator; accelerating germination: is placed in a climatic chamber.
Preferably: the germination accelerating conditions in the step (2) are as follows: day 25 ℃,12 hours, and illumination intensity of 100 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 70-80%; the culture dish cover was opened 1 time a day during germination, and distilled water was appropriately supplemented depending on the wet condition of the germination paper.
Preferably: step (3) perlite particle size: 1-3 mm; vermiculite particle size: 1-3 mm; peat fibers with the length of 0-6 mm and the pH of 5.5-6.0; and (3) disinfection: the carbendazim or potassium permanganate is adopted to be sprayed and disinfected according to the conventional dosage.
Preferably: the step (4) is specifically as follows: when the honeysuckle flower seed germinates until the length of the radicle is equal to the length of the seed, vertically placing the radicle into the hole, enabling the hypocotyl to be level with the surface of the matrix, and then extruding the surrounding matrix to enable the radicle to be tightly combined with the matrix.
Preferably: step (5) further comprises: when the soil surface is dry, pouring tap water for removing a large amount of salt ions, so that the EC value is between 0.015 and 0.030ms/cm.
Preferably: step (6) further comprises: and (5) fertilizing regularly according to the fertilizer use requirement.
The invention also provides application of any one of the methods in forestry breeding.
Compared with the prior art, the invention discloses a method for improving the germination rate and the survival rate of seedlings of lonicera fragrantis seeds, and the obtained technical effects are that the germination rate, the survival rate of seedlings and the quality of seedlings are obviously improved, and the population quantity is greatly increased.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.
FIG. 1 is a drawing of a representative plant of the treatments provided by the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The embodiment of the invention discloses a method for improving germination rate and seedling survival rate of lonicera macranthoides seeds.
Example 1
A method for improving germination rate and seedling survival rate of lonicera macranthoides seeds comprises the following steps:
collecting mature lonicera macranthoides fruits in 10 months, and naturally air-drying the fruits in an environment without direct sunlight. Removing pulp after the fruits are dried and shrunk;
the seeds are placed in the middle of 3 layers of sterile cotton germination accelerating paper respectively at the upper and lower layers, distilled water is dripped to saturate the germination accelerating paper with water, and then the seeds are placed in a culture dish and placed in a seed incubator at 25 ℃. After 24h of seed soaking, the seeds are taken out of the germination accelerating paper and placed on 2 layers of sterile cotton germination accelerating paper, placed in a culture dish, and distilled water is dripped until the germination accelerating paper is saturated in water absorption, and no obvious water accumulation exists in the culture dish. Keeping the culture dish closed by 90%, and placing the culture dish in a climatic chamber for germination, wherein the environmental conditions are as follows: day 25 ℃,12 hours, and illumination intensity of 100 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 70-80%. The culture dish cover is opened for 1 time every day during the germination accelerating period, and distilled water is properly supplemented according to the wet condition of germination accelerating paper;
perlite (particle size 1-3 mm): vermiculite (particle size 1-3 mm): peat (fiber length 0-6 mm, pH 5.5-6.0) with volume ratio 1:1:2.5, adopting carbendazim or potassium permanganate to spray and sterilize according to the conventional dosage, filling the mixture into a container, pouring water, and digging a hole with the diameter of 2-3 mm and the depth of 1-1.5 cm on the surface of the medium in the middle of the container for standby. When the seeds of lonicera flos caryophyllata sprout until the length of radicle is equal to the length of the seed, only the radicle is embedded into the matrix (vertically placed in the hole), the hypocotyl is flush with the surface of the matrix, and then the surrounding matrix is extruded, so that the radicle and the matrix are tightly combined.
Placing planted seedlings in a climatic chamber under the following environmental conditions: day 28 ℃,12 hours, and illumination intensity 300 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 60-70%. When the soil surface is dry, tap water (EC value between 0.015-0.030 ms/cm) with a large amount of salt ions removed is irrigated.
When the cotyledons of the lonicera flos lonicerae seedlings begin to wither, the substrate is on the surface layer according to 0.026g/cm 2 N is applied in the ratio of: p: the mass ratio of K is 1:1:1 and applying fertilizer regularly according to the fertilizer application requirement.
Comparative test 1
To verify the best mode of seed soaking, 60 seeds are equally divided into 3 parts, and 3 treatments are respectively carried out:
1. immersing the dried lonicera macranthoides seeds in distilled water;
2. placing the dried lonicera macranthoides seeds in the shade on 3 layers of sterile cotton germination accelerating paper, placing the seeds in a culture dish together, and dripping distilled water to saturate the germination accelerating paper with water;
3. the method comprises the steps of placing the dried lonicera macranthoides seeds in the middle of sterile cotton germination accelerating paper with 3 layers at the upper and lower parts respectively, placing the germination accelerating paper in a culture dish, and dripping distilled water to saturate the germination accelerating paper with water.
Then, 3 treatments are all placed in a seed incubator at 25 ℃, seeds are taken out from the germination accelerating paper after soaking for 24 hours, placed on 2 layers of sterile cotton germination accelerating paper, placed in a culture dish, distilled water is dripped until the germination accelerating paper is saturated in water absorption, and no obvious ponding exists in the culture dish. Keeping the culture dish closed by 90%, and placing the culture dish in a climatic chamber for germination, wherein the environmental conditions are as follows: day 25 ℃,12 hours, and illumination intensity of 100 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Air humidity 70-to-70%80%. The culture dish cover was opened 1 time a day during germination, and distilled water was appropriately supplemented depending on the wet condition of the germination paper. Each treatment was repeated 3 times.
After 1 month, the germination rate of each treatment was counted, and specific data are shown in Table 1, and it can be seen that the germination rate was highest by immersing seeds of lonicera macranthoides in the middle of 3 layers of sterile cotton germination accelerating paper on the upper and lower sides.
TABLE 1 influence of seed soaking modes on germination rate
Comparative test 2
To verify the optimal time for soaking, 80 pieces of dry lonicera macranthoides seeds in the shade are placed between 3 layers of sterile cotton germination accelerating paper on the upper and lower sides, placed in a culture dish, and distilled water is dripped to saturate the germination accelerating paper with water for soaking the seeds (different treatment time).
Thereafter, every 12 hours, the seed mass was measured until the seed mass of 2 interval measurements no longer increased. 20 seeds are selected during each measurement, are placed on a layer of 2 sterile cotton germination accelerating paper, are placed in a culture dish, and are dripped with distilled water until the germination accelerating paper is saturated in water absorption, and no obvious ponding exists in the culture dish. Keeping the culture dish closed by 90%, and placing the culture dish in a climatic chamber for germination, wherein the environmental conditions are as follows: day 25 ℃,12 hours, and illumination intensity of 100 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 70-80%. The culture dish cover was opened 1 time a day during germination, and distilled water was appropriately supplemented depending on the wet condition of the germination paper. Each treatment was repeated 3 times.
After 1 month, the germination rate of each treatment is counted, and specific data are shown in Table 2, and the soaking time of lonicera macranthoides seeds is preferably 24 hours.
TABLE 2 seed quality measured at different seed soaking times
Comparative test 3
In order to verify the optimal environmental conditions for germination, 80 seeds of lonicera macranthoides seeds after seed soaking are equally divided into 4 groups, the 4 groups of seeds are placed on 2 layers of sterile cotton germination accelerating paper, the germination accelerating paper is placed in a climatic chamber for germination accelerating, and 4 treatments are arranged in the germination accelerating environment:
1. keeping the dish closed by 90% and the environmental conditions are: day 25 ℃,12 hours, and illumination intensity of 100 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 70-80%;
2. closing the culture dish, wherein the environmental conditions are as follows: day 25 ℃,12 hours, and illumination intensity of 100 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 70-80%;
3. keeping the dish closed by 90% and the environmental conditions are: day 25 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 70-80%;
4. keeping the dish closed by 90% and the environmental conditions are: day 15 ℃,12 hours, and the illumination intensity is 100 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 70-80%. Each treatment was repeated 3 times.
After 1 month, the germination rate of each treatment is counted, the specific data are shown in Table 3, and the optimal environmental condition of lonicera macranthoides seeds is 90% of the closed culture dish, and the environmental condition is: day 25 ℃,12 hours, and illumination intensity of 100 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 70-80%.
TABLE 3 germination rates under different germination Environment conditions
Comparative test 4
To verify the best matrix mix for seeding, 3 matrix mix were set:
1. perlite (particle size 1-3 mm): vermiculite (particle size 1-3 mm): peat (fiber length 0-6 mm, pH 5.5-6.0) with volume ratio 1:1:2.5, mixing the materials into a matrix;
2. perlite (particle size 1-3 mm): vermiculite (particle size 1-3 mm): peat (fiber length 0-6 mm, pH 5.5-6.0) with volume ratio 1:0:2.5, mixing the materials into a matrix;
3. perlite (particle size 1-3 mm): vermiculite (particle size 1-3 mm): peat (fiber length 0-6 mm, pH 5.5-6.0) with volume ratio of 0:1:2.5, and mixing the materials into a matrix.
Then after the 3 substrates were sterilized, 20 pieces of germinated honeysuckle seeds were sown. Each treatment was repeated 3 times.
After 1 month, the survival rate of each treatment is counted, the specific data are shown in Table 4, and the optimal matrix proportion for sowing lonicera macranthoides is shown as follows: perlite (particle size 1-3 mm): vermiculite (particle size 1-3 mm): peat (fiber length 0-6 mm, pH 5.5-6.0) with volume ratio 1:1:2.5, and mixing the materials into a matrix.
TABLE 4 germination rates under different germination Environment conditions
Comparative test 5
To verify the optimal environmental conditions for seedling cultivation, 4 environmental configurations were set:
1. day 28 ℃,12 hours, and illumination intensity 300 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 60-70%;
2. day 20 ℃,12 hours, and illumination intensity of 300 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Empty spaceThe air humidity is 60-70%;
3. day 28 ℃,12 hours, and the illumination intensity is 100 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 60-70%;
4. day 28 ℃,12 hours, and illumination intensity 300 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 30-40%.
Under different environmental conditions, 10 lonicera macranthoides seedlings were cultivated each, and each treatment was repeated 3 times.
The plant height, ground diameter and leaf status of each treatment were counted after 1 month, and specific data are shown in table 5. The optimal environmental conditions for cultivation of lonicera macranthoides seedlings are as follows: day 28 ℃,12 hours, and illumination intensity 300 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 60-70%.
TABLE 5 seedling State under different environmental conditions
Comparative test 6
To verify the optimal fertilization formula for seedling cultivation, when the cotyledons of lonicera macranthoides seedlings begin to wither, 4 fertilization treatments were set:
1. the surface matrix was in the order of 0.026g/cm 2 Applying N: p: the mass ratio of K is 1:1:1, a slow-release mixed fertilizer;
2. the surface matrix was in the order of 0.052g/cm 2 Applying N: p: the mass ratio of K is 1:1:1, a slow-release mixed fertilizer;
3. the surface matrix was in the order of 0.013g/cm 2 Applying N: p: the mass ratio of K is 1:1:1, a slow-release mixed fertilizer;
4. no fertilizer is applied.
Under 4 fertilization conditions, 10 lonicera macranthoides seedlings were cultivated each, and each treatment was repeated 3 times. After 1 month, the plant height, ground diameter and leaf status of each treatment were counted, and specific data are shown in Table 6, everywhereA picture of a representative plant is shown in FIG. 1 (left and right). The optimal fertilization formula for cultivating lonicera macranthoides seedlings is as follows: when the cotyledons of the lonicera flos lonicerae seedlings begin to wither, the substrate is on the surface layer according to 0.026g/cm 2 Applying N: p: the mass ratio of K is 1:1: 1.
TABLE 6 seedling State under different fertilization conditions
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (9)
1. A method for improving germination rate and seedling survival rate of lonicera macranthoides seeds, which is characterized by comprising the following steps:
(1) Collecting mature lonicera macranthoides fruits, and preprocessing to obtain seeds;
(2) Placing the seeds obtained in the step (1) between 3 layers of sterile cotton germination accelerating paper on the upper and lower sides, dripping distilled water to saturate the germination accelerating paper with water, then placing the seeds into a culture dish, soaking the seeds in the environment of 25 ℃ for 24 hours, taking the seeds out of the germination accelerating paper, placing the seeds on 2 layers of sterile cotton germination accelerating paper laid on the culture dish, dripping distilled water until the germination accelerating paper is saturated with water, and no obvious water accumulation exists in the culture dish; keeping the culture dish closed by 90%, accelerating germination, and periodically supplementing water;
(3) Perlite, vermiculite and peat are mixed according to the volume ratio of 1:1:2.5, mixing the materials into a matrix, sterilizing, filling the matrix into a container, pouring enough water, and digging a hole with the diameter of 2-3 mm and the depth of 1-1.5 cm on the surface of the matrix in the middle of the container for standby;
(4) After the seeds of lonicera macranthoides sprout, burying radicle of lonicera macranthoides into the hole in the step (3);
(5) Placing planted seedlings in a climatic chamber under the following environmental conditions: day 28 ℃,12 hours, and illumination intensity 300 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 60-70%;
(6) When the cotyledons of the lonicera flos lonicerae seedlings begin to wither, the substrate is on the surface layer according to 0.026g/cm 2 N is applied in the ratio of: p: the mass ratio of K is 1:1: 1.
2. The method of claim 1, wherein the preprocessing of step (1): naturally air-drying the fruit in the environment without direct sunlight, and removing pulp after the fruit is dried and shrunk.
3. The method of claim 2, wherein the environment of step (2) is: placing in a seed incubator; the germination accelerating: is placed in a climatic chamber.
4. A method according to claim 3, wherein the germination accelerating conditions of step (2) are: day 25 ℃,12 hours, and illumination intensity of 100 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the Night 15 ℃,12 hours, and illumination intensity of 0 mu mol.m -2 ·s -1 The method comprises the steps of carrying out a first treatment on the surface of the The air humidity is 70-80%; the culture dish cover was opened 1 time a day during germination, and distilled water was appropriately supplemented depending on the wet condition of the germination paper.
5. The method of claim 4, wherein the perlite in step (3) has a particle size of: 1-3 mm; the vermiculite has the particle size: 1-3 mm; the length of the peat fibers is 0-6 mm, and the pH value is 5.5-6.0; the disinfection: the carbendazim or potassium permanganate is adopted to be sprayed and disinfected according to the conventional dosage.
6. The method of claim 5, wherein step (4) is specifically: when the honeysuckle flower seed germinates until the length of the radicle is equal to the length of the seed, vertically placing the radicle into the hole, enabling the hypocotyl to be level with the surface of the matrix, and then extruding the surrounding matrix to enable the radicle to be tightly combined with the matrix.
7. The method of claim 6, wherein step (5) further comprises: when the soil surface is dry, pouring tap water for removing a large amount of salt ions, so that the EC value is between 0.015 and 0.030ms/cm.
8. The method of claim 7, wherein step (6) further comprises: and (5) fertilizing regularly according to the fertilizer use requirement.
9. Use of the method of any one of claims 1-8 in forestry breeding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310112137.5A CN115989784A (en) | 2023-02-14 | 2023-02-14 | Method for improving germination rate and seedling survival rate of lonicera macranthoides seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310112137.5A CN115989784A (en) | 2023-02-14 | 2023-02-14 | Method for improving germination rate and seedling survival rate of lonicera macranthoides seeds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115989784A true CN115989784A (en) | 2023-04-21 |
Family
ID=85990196
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310112137.5A Pending CN115989784A (en) | 2023-02-14 | 2023-02-14 | Method for improving germination rate and seedling survival rate of lonicera macranthoides seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115989784A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117378317A (en) * | 2023-12-11 | 2024-01-12 | 潍坊市农业科学院(山东省农业科学院潍坊市分院) | Method for improving germination rate of aged seeds of clove |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102612889A (en) * | 2012-04-09 | 2012-08-01 | 常熟市海虹苗木花卉有限公司 | Method for increasing seedling survival rate of Lonicera sempervirens seeds |
| CN103283345A (en) * | 2013-06-04 | 2013-09-11 | 中国农业科学院棉花研究所 | Method for identifying activity of cotton seeds |
| CN105210625A (en) * | 2015-10-09 | 2016-01-06 | 陕西省西安植物园 | A kind ofly pass through a moon method for Seeds pression nursery |
| CN105359815A (en) * | 2015-12-15 | 2016-03-02 | 中国农业科学院棉花研究所 | Method for identifying cold resistance of cotton in germination period |
| CN115486317A (en) * | 2022-10-28 | 2022-12-20 | 乌兰察布市农林科学研究所 | Efficient seedling raising technology for lonicera caerulea |
-
2023
- 2023-02-14 CN CN202310112137.5A patent/CN115989784A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102612889A (en) * | 2012-04-09 | 2012-08-01 | 常熟市海虹苗木花卉有限公司 | Method for increasing seedling survival rate of Lonicera sempervirens seeds |
| CN103283345A (en) * | 2013-06-04 | 2013-09-11 | 中国农业科学院棉花研究所 | Method for identifying activity of cotton seeds |
| CN105210625A (en) * | 2015-10-09 | 2016-01-06 | 陕西省西安植物园 | A kind ofly pass through a moon method for Seeds pression nursery |
| CN105359815A (en) * | 2015-12-15 | 2016-03-02 | 中国农业科学院棉花研究所 | Method for identifying cold resistance of cotton in germination period |
| CN115486317A (en) * | 2022-10-28 | 2022-12-20 | 乌兰察布市农林科学研究所 | Efficient seedling raising technology for lonicera caerulea |
Non-Patent Citations (1)
| Title |
|---|
| 和子森;陈苏依勒;程明;温晋芳;蔡靖;姜在民;: "濒危植物羽叶丁香种子休眠与萌发特性研究", 植物生理学报, no. 04, 20 April 2016 (2016-04-20), pages 560 - 568 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117378317A (en) * | 2023-12-11 | 2024-01-12 | 潍坊市农业科学院(山东省农业科学院潍坊市分院) | Method for improving germination rate of aged seeds of clove |
| CN117378317B (en) * | 2023-12-11 | 2024-04-02 | 潍坊市农业科学院(山东省农业科学院潍坊市分院) | Method for improving germination rate of aged seeds of clove |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103141265B (en) | Pansy-industrialized plug seedling method | |
| CN100426952C (en) | Tissue culture seedling-growing method for bird's-net fern | |
| CN107135927B (en) | Cuttage and rapid propagation method for alum roots | |
| CN101084716A (en) | Multi-strain co-planting seedlings growing method for ornamental plant | |
| CN105519417A (en) | Method for raising seedlings in plugs by cutting | |
| CN103125386B (en) | Industrial horseradish planting method | |
| CN102511283A (en) | Method of artificially rearing seedlings of Cardamine hupingshanensis | |
| CN115226615A (en) | Rare rhododendron seed breeding method | |
| KR20190055706A (en) | Method for cultivating high quality Astragalus membranaceus plug seedling using nutrition solution having controlled electrical conductivity | |
| CN106613274B (en) | Aconitum sinomontanum seed seedling raising method | |
| CN115989784A (en) | Method for improving germination rate and seedling survival rate of lonicera macranthoides seeds | |
| CN110692472A (en) | Method for field transplanting and breeding stock seeds of potato low latitude plateau tissue culture seedlings | |
| CN103039240B (en) | Seeding and seedling raising method for waterbirch in Three Gorges Reservoir region | |
| CN107278421A (en) | A kind of seed growing method of P. delavayi | |
| CN117136782B (en) | Container seedling raising method for introducing coastal pine postsetting seeds in north | |
| CN109452127B (en) | Seedling raising substrate for improving emergence rate of fructus aurantii and application thereof | |
| CN109511534B (en) | Berry seed sowing and breeding method | |
| CN114586596B (en) | Breeding method of special ornamental plant Libo rhododendron seed in China | |
| CN107409896B (en) | Sowing and seedling raising method for golden panda trees | |
| CN103081606B (en) | Method for sugarcane spica seed sprouting and sprouted seedling temporary-planting | |
| CN109479692A (en) | A kind of citrus being suitable under laboratory condition grows directly from seeds the device and method of seedling water planting | |
| Sharma et al. | Growing media and cow urine influence the seed germination and seedling growth of papaya (Carica papaya L.). | |
| CN109769676B (en) | Soilless culture method for promoting rapid flowering of rhododendron lapponicum good seedlings | |
| Herrera et al. | Optimization of substrate and nutrient solution for organic cultivation of medicinal transplants in multicell flats | |
| Park et al. | Influence of pre-plant root substrate nitrogen levels on growth of selected fruit bearing vegetable seedlings in cylindrical paper pots |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |