CN102511283A - Method of artificially rearing seedlings of Cardamine hupingshanensis - Google Patents
Method of artificially rearing seedlings of Cardamine hupingshanensis Download PDFInfo
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Abstract
The invention discloses a method of artificially rearing seedlings of Cardamine hupingshanensis, which is characterized by including steps: firstly, soaking seeds, namely soaking seeds of Cardamine hupingshanensis for 1 to 30 hours with soaking fluid, pouring the soaking fluid, and washing the seeds with water; secondly, accelerating germination, namely moving the soaked seeds to gauze, washing the seeds with germination fluid for 1-10 minutes, keeping the seeds humid and ventilated, and washing the seeds with the germination fluid once a day for 1-10 minutes to obtain germinating seeds of Cardamine hupingshanensis; and thirdly, seeding and rearing seedlings, namely sowing germinated seeds into seedling trays, placing the seeding trays at a place with the temperature 10-25 DEG C, the relative humidity 60%-95% and the illumination intensity 1000-3000lx, and irrigating the seedlings with irrigation fluid once to four times a day, and rearing the seedlings for 30-40 days and transferring the seedlings to a field when each seedling of Cardamine hupingshanensis grow 3-4 true leaves. By the method, seed germination rate and seedling survivals rate of Cardamine hupingshanensis are increased, and phyisologicla conditions of the seedlings are evidently superior to those of seedlings with unknown selenium content.
Description
Technical field
The present invention relates to a kind of wild plant---the artificial raise seedling method of tall bottle with spout Hairy Bittercress.Belong to the agricultural planting technique field
Technical background
Tall bottle with spout Hairy Bittercress (Cardamine hupingshanensis K.M.Liu.L.B.Chen.H.F.Bai&L.H.Liu); The Cruciferae Cardamine; Life in 2 years or herbaceos perennial survive the winter; Shady wet, the non-refractory of happiness low temperature, the adult branch in the back of blooming is prone to withered in being higher than 30 ℃ of environment, and seed is difficult to sprout in being higher than 30 ℃ of environment.The tall bottle with spout Hairy Bittercress is a kind of wild vegetable, and its delicious flavour is of high nutritive value, and is an artificial cultivation novel vegetable that is worthy to be popularized.
The tall bottle with spout Hairy Bittercress is a kind of plant of accumulating of selenium, and the highest Se content of tall bottle with spout Hairy Bittercress under the natural environment can reach 800mg/kg.Tall bottle with spout Hairy Bittercress edible can be used as the high selenium material of a kind of high-quality simultaneously, is used for food interpolation or feed and adds.But because the tall bottle with spout Hairy Bittercress is higher to the growing environment conditional request, its in the original producton location distribution very narrow, live in narrow mountain stream streams or the moistening rock of noss grown mountain stream, flowing water throughout the year, water quality is limpid.Wild tall bottle with spout Hairy Bittercress seed maturity is 6～July, and seed germination is extremely difficult under field conditions (factors) to be sprouted and becomes seedling, and seedling stage is longer, about needs 3～4 months under the natural conditions, and the high temperature in 8～September causes its seedling mortality.Its modes of reproduction is mainly that the breeding of ripe plant nutrition is main in the former habitat, and diffusion velocity and the scope of population are all very little.
Hunan Normal University's Master's thesis " research that the tall bottle with spout Hairy Bittercress is bred " (Dai Jianping, 2002) disclose to the vigor of tall bottle with spout Hairy Bittercress seed under the different holding conditions preserve, the research of the characteristic of growing of different stage of ripeness seed germination situation and tall bottle with spout Hairy Bittercress different phase etc.But in this paper the method that discloses a kind of suitable tall bottle with spout Hairy Bittercress artificial breeding.
" the sprouting characteristic research of tall bottle with spout Hairy Bittercress seed " (Bai Hongfeng etc.; 2010, Anhui agricultural science) when disclosing in the tall bottle with spout Hairy Bittercress seedling raising process, should account temperature and the factor of illumination; The germination process temperature should not surpass 25 ℃, and seed is preserved the rearing condition that needs low temperature drying.But concrete steps and method to tall bottle with spout Hairy Bittercress artificial breeding do not relate to.Therefore, through artificial propagation tall bottle with spout Hairy Bittercress, producing in batches, is the necessary ways of this precious resources of development and use.The present invention therefore.
Summary of the invention
The present invention provides a kind of artificial raise seedling method of tall bottle with spout Hairy Bittercress, but goes out to be fit to the seedling of field-transplanting with tall bottle with spout Hairy Bittercress cultivating seeds with the method scale.
Technical scheme of the present invention is following:
A kind of artificial raise seedling method of tall bottle with spout Hairy Bittercress is characterized in that said method comprising the steps of:
(1) seed soaking step: soak tall bottle with spout Hairy Bittercress seed 1～30h with seed-soaking liquid, outwell seed-soaking liquid, water flushing seed;
(2) vernalization step: the back seed of will soaking seed is transferred on the gauze, and with vernalization liquid flushing seed, washing time is 1～10 minute, keeps the moistening ventilation of seed, and every day, washing time was 1～10 minute tall bottle with spout Hairy Bittercress seed that obtains sprouting with vernalization liquid flushing one time;
(3) seeding and seedling raising step: the tall bottle with spout Hairy Bittercress seed that sprouts after the vernalization is broadcast into seedling-cultivating tray, and it is 10～25 ℃ that seedling-cultivating tray is placed temperature, and relative moisture is 60%～95%, and intensity of illumination is in 1000～3000lx environment, waters irrigation liquid every day 1～4 time; Cultivated 30～40 days, tall bottle with spout Hairy Bittercress seedling grows 3～4 true leaves can transplant the land for growing field crops.
Preferably, the seed-soaking liquid that adopts in the said method comprises that selenium concentration is the selenium source of 1～100 μ g/L.
Preferably, the plant hormone and single the kind or multiple mineral element that also comprise effective vernalization amount in the seed-soaking liquid that adopts in the said method.
Preferably, also comprise the gibberellin of concentration in the seed-soaking liquid that adopts in the said method at 1～50mg/L.
Preferably, the selenium concentration of selenium source is 5～50 μ g/L in the said method; The concentration of said gibberellin is at 5～20mg/L.
Preferably, contain selenium source in the vernalization liquid in the said method, selenium concentration is 1～100 μ g/L in the said vernalization liquid.
Preferably, contain selenium source in the irrigation liquid in the said method, selenium concentration is 0.01～10mg/L in the said irrigation liquid.
Preferably, selenium source is selected from one or more combination in any of selenite, selenate and seleno-amino acids in the said method.
Preferably, seedling-cultivating tray is selected the cave dish for use in the said method, and the environmental temperature of using of growing seedlings is 15～20 ℃, and relative air humidity is 80%～90%, and intensity of illumination is 1500～2000lx.
Preferably, seed carries out seed disinfection in the said method before the seed soaking step, and the step of said seed disinfection comprises with thimerosal soaks 1～60s, the step of then thimerosal being rinsed well with tall bottle with spout Hairy Bittercress seed.
The present invention comprises with the step that tall bottle with spout Hairy Bittercress cultivating seeds goes out to be fit to the field-transplanting seedling: seed disinfection, seed soaking, vernalization, the several steps of sowing, grow seedlings.The used thimerosal of the present invention, seed-soaking liquid, vernalization spray liquid, the irrigation liquid of growing seedlings, and the used vessel that directly contact with seed in the invention, matrix, the trial period all puts constant temperature to 10 in the insulating box～25 ℃ in advance, preferably constant temperature to 15～20 ℃.
The seed disinfection step comprises:
With thimerosal tall bottle with spout Hairy Bittercress seed is soaked 1～60s, preferred 20～40s.Thimerosal is rinsed well.Thimerosal adopts the sterilization of 70%～75% alcohol water blend.The volatile heat absorption of alcohol reduces the surface of the seed temperature, helps keeping seed vitality.
The seed soaking step comprises:
Soak tall bottle with spout Hairy Bittercress seed 1～30h, preferred 6～24h with seed-soaking liquid.Outwell seed-soaking liquid then, water flushing seed 5～6 times.Tall bottle with spout Hairy Bittercress seed-soaking liquid is a Se solution, and said Se solution can be inorganic selenium solution (selenite solution or a selenate solution), also can be organic selenium solution (seleno-amino acids solution), or both is with the mixing of any ratio.Said Se solution concentration be 1～100 μ g/L contain the selenium aqueous solution.Be preferably that selenium concentration is 5～50 μ g/L in the seed-soaking liquid.
The plant hormone (gibberellin, methyl etc. can promote the seed germination parahormone) and the single of suitable amount that can add suitable concentration in the tall bottle with spout Hairy Bittercress seed-soaking liquid simultaneously plant or multiple mineral element.As preferably, plant hormone is used gibberellin in the tall bottle with spout Hairy Bittercress seed-soaking liquid, and interpolation concentration is 1～50mg/L, and more preferably gibberellin concentration is 5～20mg/L.
The vernalization step comprises:
The back seed of will soaking seed is transferred on the gauze, and with vernalization liquid flushing seed, washing time is 1～10 minute, preferred 3～7 minutes.Keep the moistening ventilation of seed 3 days, every day, washing time was identical with the liquid of vernalization for the first time washing time with vernalization liquid flushing one time.
The used vernalization liquid of the present invention is Se solution, and said Se solution can be inorganic selenium solution (selenite solution or a selenate solution), also can be organic selenium solution (seleno-amino acids solution), or both is with the mixing of any ratio.Said Se solution selenium concentration is 1～100 μ g/L, is preferably that selenium concentration is 5～50 μ g/L in the vernalization liquid.
The seeding and seedling raising step comprises:
The tall bottle with spout Hairy Bittercress seed that sprouts after the vernalization is broadcast into seedling-cultivating tray, and it is 10～25 ℃ that seedling-cultivating tray is placed temperature, and relative moisture is 60%～95%, and intensity of illumination is in 1000～3000lx environment, waters irrigation liquid every day 1～4 time.Cultivated 30～40 days, tall bottle with spout Hairy Bittercress seedling grows 3～4 true leaves can transplant the land for growing field crops.
As preferably, seedling-cultivating tray is selected the cave dish for use, and seedling medium is that the content of organic matter is high, and air capacity of soils is good.Environmental temperature is preferably 15～20, and relative air humidity is preferred 80%～90%, and intensity of illumination is 1500～2000lx.The used irrigation liquid of the present invention is a Se solution, and preferred selenium concentration is the aqueous solution of 0.01～10mg/L, more preferably selenium concentration 0.02～5mg/L in the irrigation liquid.
Compare prior art, the present invention has following beneficial effect:
1, with various vessel and preparation used in the seedling raising process, through constant temperature in advance to the preference temperature of tall bottle with spout Hairy Bittercress seed and seedling.For the comparatively responsive tall bottle with spout Hairy Bittercress of high temperature, suitable temperature is its seed germination and the necessary condition of seedling growth.The various utensils in the seedling raising process and the adjusting of formulation temperature make the environment of growing seedlings at utmost near tall bottle with spout Hairy Bittercress primitive environment, and this processing process can significantly promote tall bottle with spout Hairy Bittercress seed germination rate and seedling percent.
2, the seed-soaking liquid and the vernalization liquid of the present invention's use are certain density Se solution, and temperature is suitable, can significantly strengthen its seed vitality and germination rate.
3, the present invention is in tall bottle with spout Hairy Bittercress growth of seedling process; The higher selenium solution of working concentration is as irrigation liquid; Find that through experiment selenium has tangible promotion growth effect to tall bottle with spout Hairy Bittercress seedling, shortened its seedling-growing time, and can significantly promote its growth of seedlings speed; And use certain density selenium solution as irrigation liquid, the physiological situation of its seedling obviously is superior to not adding the seedling of selenium.
Embodiment:
Following examples are used to explain the present invention, but can not be used for limiting scope of the present invention.The implementation condition that adopts among the embodiment can be done further adjustment according to the condition of concrete producer, and unaccounted implementation condition is generally the condition in the normal experiment.
Embodiment 1
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Carry out disinfection with conventional short time hot-water soak, use vessel without Temperature Treatment, water etc. to carry out seed-soaking, vernalization, seeding and seedling raising, after planting seedling raising process is same adopts clear water without Temperature Treatment as irrigation liquid.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 2
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 10 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.It is 10 ℃ clear water that seed-soaking liquid, vernalization liquid use through Temperature Treatment, and after planting seedling raising process adopts 10 ℃ of clear water through Temperature Treatment as irrigation liquid.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 3
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 15 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.It is 15 ℃ clear water that seed-soaking liquid, vernalization liquid use through Temperature Treatment, and after planting seedling raising process adopts 15 ℃ of clear water through Temperature Treatment as irrigation liquid.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 4
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 20 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.It is 20 ℃ clear water that seed-soaking liquid, vernalization liquid use through Temperature Treatment, and after planting seedling raising process adopts 20 ℃ of clear water through Temperature Treatment as irrigation liquid.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 5
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 25 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.It is 25 ℃ clear water that seed-soaking liquid, vernalization liquid use through Temperature Treatment, and after planting seedling raising process adopts 25 ℃ of clear water through Temperature Treatment as irrigation liquid.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 1～5 experimental result is seen table 1.Wherein emergence rate is after planting 10 days statistical datas.Plant height, root length, dry matter are after planting 30 days statistical datas.
Table 1
Embodiment 6
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 20 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.Seed-soaking liquid, vernalization liquid use through Temperature Treatment be 20 ℃ 1 μ g/L contain the selenium aqueous solution, after planting seedling raising process adopts 20 ℃ of clear water through Temperature Treatment as irrigation liquid.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 7
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 20 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.Seed-soaking liquid, vernalization liquid use through Temperature Treatment be 20 ℃ 5 μ g/L contain the selenium aqueous solution, after planting seedling raising process adopts 20 ℃ of clear water through Temperature Treatment as irrigation liquid.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 8
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 20 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.Seed-soaking liquid, vernalization liquid use through Temperature Treatment be 20 ℃ 10 μ g/L contain the selenium aqueous solution, after planting seedling raising process adopts 20 ℃ of clear water through Temperature Treatment as irrigation liquid.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 9
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 20 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.Seed-soaking liquid, vernalization liquid use through Temperature Treatment be 20 ℃ 20 μ g/L contain the selenium aqueous solution, after planting seedling raising process adopts 20 ℃ of clear water through Temperature Treatment as irrigation liquid.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 10
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 20 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.Seed-soaking liquid, vernalization liquid use through Temperature Treatment be 20 ℃ 50 μ g/L contain the selenium aqueous solution, after planting seedling raising process adopts 20 ℃ of clear water through Temperature Treatment as irrigation liquid.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 11
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 20 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.Seed-soaking liquid, vernalization liquid use through Temperature Treatment be 20 ℃ 100 μ g/L contain the selenium aqueous solution, after planting seedling raising process adopts 20 ℃ of clear water through Temperature Treatment as irrigation liquid.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 6～11 experimental results are seen table 2.Wherein emergence rate is after planting 10 days statistical datas.Plant height, root length, dry matter are after planting 30 days statistical datas.
Table 2
Embodiment 12
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 20 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.Seed-soaking liquid, vernalization liquid use through Temperature Treatment be 20 ℃ 20 μ g/L contain the selenium aqueous solution, after planting seedling raising process adopts and contains the selenium aqueous solution as irrigation liquid through 20 ℃ of 0.01mg/L of Temperature Treatment.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 13
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 20 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.Seed-soaking liquid, vernalization liquid use through Temperature Treatment be 20 ℃ 20 μ g/L contain the selenium aqueous solution, after planting seedling raising process adopts and contains the selenium aqueous solution as irrigation liquid through 20 ℃ of 0.05mg/L of Temperature Treatment.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 14
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 20 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.Seed-soaking liquid, vernalization liquid use through Temperature Treatment be 20 ℃ 20 μ g/L contain the selenium aqueous solution, after planting seedling raising process adopts and contains the selenium aqueous solution as irrigation liquid through 20 ℃ of 0.1mg/L of Temperature Treatment.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 15
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 20 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.Seed-soaking liquid, vernalization liquid use through Temperature Treatment be 20 ℃ 20 μ g/L contain the selenium aqueous solution, after planting seedling raising process adopts and contains the selenium aqueous solution as irrigation liquid through 20 ℃ of 1mg/L of Temperature Treatment.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 16
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 20 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.Seed-soaking liquid, vernalization liquid use through Temperature Treatment be 20 ℃ 20 μ g/L contain the selenium aqueous solution, after planting seedling raising process adopts and contains the selenium aqueous solution as irrigation liquid through 20 ℃ of 2mg/L of Temperature Treatment.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 17
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 20 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.Seed-soaking liquid, vernalization liquid use through Temperature Treatment be 20 ℃ 20 μ g/L contain the selenium aqueous solution, after planting seedling raising process adopts and contains the selenium aqueous solution as irrigation liquid through 20 ℃ of 5mg/L of Temperature Treatment.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 18
Get 300 tall bottle with spout Hairy Bittercress seeds, be divided into 3 groups.Use is 20 ℃ 75% alcohol disinfecting seed through Temperature Treatment in advance.Seed-soaking liquid, vernalization liquid use through Temperature Treatment be 20 ℃ 20 μ g/L contain the selenium aqueous solution, after planting seedling raising process adopts and contains the selenium aqueous solution as irrigation liquid through 20 ℃ of 10mg/L of Temperature Treatment.Write down seed germination rate, seedling-growing time, emergence rate in the experimentation, after the completion of growing seedlings, record seedling plant height, long, the dry matter of root.
Embodiment 12～18 experimental results are seen table 3.Wherein emergence rate is after planting 10 days statistical datas.Plant height, root length, dry matter are after planting 30 days statistical datas.
Table 3
Above-mentioned instance only is explanation technical conceive of the present invention and characteristics, and its purpose is to let the people who is familiar with this technology can understand content of the present invention and enforcement according to this, can not limit protection scope of the present invention with this.All equivalent transformations that spirit is done according to the present invention or modification all should be encompassed within protection scope of the present invention.
Claims (10)
1. the artificial raise seedling method of a tall bottle with spout Hairy Bittercress is characterized in that said method comprising the steps of:
(1) seed soaking step: soak tall bottle with spout Hairy Bittercress seed 1 ~ 30 h with seed-soaking liquid, outwell seed-soaking liquid, water flushing seed;
(2) vernalization step: the back seed of will soaking seed is transferred on the gauze, and with vernalization liquid flushing seed, washing time is 1 ~ 10 minute, keeps the moistening ventilation of seed, and every day, washing time was 1 ~ 10 minute tall bottle with spout Hairy Bittercress seed that obtains sprouting with vernalization liquid flushing one time;
(3) seeding and seedling raising step: the tall bottle with spout Hairy Bittercress seed that sprouts after the vernalization is broadcast into seedling-cultivating tray, and it is 10 ~ 25 ℃ that seedling-cultivating tray is placed temperature, and relative moisture is 60% ~ 95%, and intensity of illumination is in 1000 ~ 3000 lx environment, waters irrigation liquid every day 1 ~ 4 time; Cultivated 30 ~ 40 days, tall bottle with spout Hairy Bittercress seedling grows 3 ~ 4 true leaves can transplant the land for growing field crops.
2. method according to claim 1 is characterized in that the seed-soaking liquid that adopts in the said method comprises that selenium concentration is the selenium source of 1 ~ 100 μ g/L.
3. method according to claim 2 also comprises the plant hormone and single the kind or multiple mineral element of effective vernalization amount in the seed-soaking liquid that it is characterized in that adopting in the said method.
4. method according to claim 2 also comprises the gibberellin of concentration at 1 ~ 50 mg/L in the seed-soaking liquid that it is characterized in that adopting in the said method.
5. method according to claim 4, the selenium concentration that it is characterized in that selenium source in the said method are 5 ~ 50 μ g/L; The concentration of said gibberellin is at 5 ~ 20 mg/L.
6. method according to claim 1 is characterized in that containing selenium source in the vernalization liquid in the said method, and selenium concentration is 1 ~ 100 μ g/L in the said vernalization liquid.
7. method according to claim 1 is characterized in that containing selenium source in the irrigation liquid in the said method, and selenium concentration is 0.01 ~ 10 mg/L in the said irrigation liquid.
8. according to claim 2 or 6 or 7 described methods, it is characterized in that selenium source in the said method is selected from one or more combination in any of selenite, selenate and seleno-amino acids.
9. method according to claim 1 is characterized in that seedling-cultivating tray is selected the cave dish for use in the said method, and the environmental temperature of using of growing seedlings is 15 ~ 20 ℃, and relative air humidity is 80% ~ 90%, and intensity of illumination is 1500 ~ 2000 lx.
10. method according to claim 1; It is characterized in that seed carries out seed disinfection in the said method before the seed soaking step; The step of said seed disinfection comprises with thimerosal soaks 1 ~ 60 s, the step of then thimerosal being rinsed well with tall bottle with spout Hairy Bittercress seed.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103053805A (en) * | 2012-11-30 | 2013-04-24 | 湖北盛硒生物科技有限公司 | Selenium-rich feed additive and preparation method thereof |
| CN103651114A (en) * | 2012-12-14 | 2014-03-26 | 湖北盛硒生物科技有限公司 | Tissue culture and rapid propagation method for cardamine hupingshanesis |
| WO2014059951A1 (en) * | 2012-10-16 | 2014-04-24 | Zheng Jianzhong | Methods for producing foods having germinated-seed activity, germinated seeds enriched with selenium, or germinated seeds enriched with trace elements |
| CN104067802A (en) * | 2014-06-19 | 2014-10-01 | 安徽百卉园林有限公司 | Method for increasing germination rate of nandina seeds |
| CN107347293A (en) * | 2017-06-20 | 2017-11-17 | 金寨新天地现代农业有限公司 | A kind of method for culturing seedlings of summer kohlrabi strong sprout |
| CN108738466A (en) * | 2018-05-14 | 2018-11-06 | 安徽过湾农业科技有限公司 | A kind of Hairy Bittercress seed seedling-raising method |
| CN110449460A (en) * | 2018-05-08 | 2019-11-15 | 中环循环境技术有限责任公司 | A kind of engineer application method using the restoring cadmium polluted agricultural land soil of tall bottle with spout Hairy Bittercress |
| CN111345202A (en) * | 2018-12-23 | 2020-06-30 | 陕西安康燚甸园聚硒植物科技开发有限公司 | Greenhouse cultivation method for cardamine hupingshanensis |
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| CN103053805A (en) * | 2012-11-30 | 2013-04-24 | 湖北盛硒生物科技有限公司 | Selenium-rich feed additive and preparation method thereof |
| CN103053805B (en) * | 2012-11-30 | 2014-04-16 | 湖北盛硒生物科技有限公司 | Selenium-rich feed additive and preparation method thereof |
| CN103651114A (en) * | 2012-12-14 | 2014-03-26 | 湖北盛硒生物科技有限公司 | Tissue culture and rapid propagation method for cardamine hupingshanesis |
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| CN107347293A (en) * | 2017-06-20 | 2017-11-17 | 金寨新天地现代农业有限公司 | A kind of method for culturing seedlings of summer kohlrabi strong sprout |
| CN110449460A (en) * | 2018-05-08 | 2019-11-15 | 中环循环境技术有限责任公司 | A kind of engineer application method using the restoring cadmium polluted agricultural land soil of tall bottle with spout Hairy Bittercress |
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