CN115486317A - Efficient seedling raising technology for lonicera caerulea - Google Patents

Efficient seedling raising technology for lonicera caerulea Download PDF

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CN115486317A
CN115486317A CN202211335886.6A CN202211335886A CN115486317A CN 115486317 A CN115486317 A CN 115486317A CN 202211335886 A CN202211335886 A CN 202211335886A CN 115486317 A CN115486317 A CN 115486317A
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seeds
seed
sand
days
lonicera caerulea
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CN115486317B (en
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田稼穑
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Ulanqab Institute Of Agriculture And Forestry
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Ulanqab Institute Of Agriculture And Forestry
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08Immunising seed
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Botany (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention provides a high-efficiency seedling raising technology for lonicera caerulea, and belongs to the technical field of lonicera caerulea seedling raising. The high-efficiency seedling raising technology for lonicera caerulea comprises the steps of seed harvesting and pretreatment, seed soaking, seed disinfection treatment, river sand treatment, seed sand mixing, temperature change treatment, hormone treatment, greenhouse environment control, nutrition pot substrate disinfection, seeding, management after seeding and transplanting, through adopting temperature change germination accelerating, namely, first medium temperature treatment and then low temperature treatment, and utilizing the modes of nutrition pot seeding and hormone treatment, the germination rate and the emergence rate of lonicera caerulea can be obviously improved, the germination rate is over 85 percent, the emergence rate is over 90 percent, and finally the purpose of improving the yield of lonicera caerulea is achieved. In addition, the lonicera caerulea cultivated in the nutrition pot is convenient to transport, the transplantation is not limited by time, the cultivation time and space of the lonicera caerulea are greatly widened, the market demand is further met, and the method is suitable for popularization and application.

Description

Efficient seedling raising technology for lonicera caerulea
Technical Field
The invention belongs to the technical field of honeysuckle seedling culture, and particularly relates to an efficient honeysuckle seedling culture technology.
Background
Lonicera caerulea L.var.edulis Turcz.ex Herd.is a variety of Lonicera caerulea of Lonicera of Caprifoliaceae. The tree shape is beautiful, the flower and the fruit can be observed, the drought resistance and the cold resistance are strong, and the tree is rare and excellent landscaping tree species. The oval purple berry has high edible value and medicinal value, is sour, sweet and delicious, contains 7 amino acids and vitamin C, can be eaten raw, can extract pigment, and can be used for brewing wine, making beverage and jam. The Lonicera edulis plant contains aucubin, has the effects of clearing away damp-heat, promoting urination, relieving pain, reducing blood pressure, protecting liver, resisting tumors and the like, can promote stem cell regeneration, obviously inhibits the replication of hepatitis B virus DNA, has a certain curative effect on treating infantile anorexia, and has extremely high economic value. The seed contains active substances such as rutin and anthocyanin, and has effects of resisting oxidation, resisting allergy, scavenging free radicals in vivo, inhibiting lipid peroxidation and platelet aggregation, preventing diabetes, reducing weight, and protecting vision.
At present, the cost of cutting propagation and tissue culture propagation of lonicera caerulea is high. In the prior art, the germination acceleration of the lonicera caerulea is realized by mixing sand and seeds, digging a pit outdoors in winter for storage for about 3-4 months, and taking out the mixture for sowing in a field, and the germination acceleration method has low germination rate of about 65 percent. Although the cost of sowing and breeding in the field is low, the emergence rate of germinated seeds is about 70 percent even in the growth process due to the restriction of climatic factors. This results in a low yield of lonicera caerulea, which cannot be put into practice in large quantities.
Disclosure of Invention
The invention aims to provide a seedling raising technology capable of remarkably improving the germination rate, emergence rate and survival rate of lonicera edulis.
The invention provides a high-efficiency seedling raising technology of lonicera caerulea, which comprises the following steps of seed harvesting and pretreatment, seed soaking, seed disinfection treatment, river sand treatment, seed and sand mixing, temperature change treatment, hormone treatment, greenhouse environment control, nutrition pot matrix disinfection, sowing, management after sowing and transplanting, and specifically comprises the following steps:
s1, seed harvesting and pretreatment: harvesting and mashing mature fruits, repeatedly rinsing with water to separate seeds from peels and pulps, drying the seeds in the shade, and selecting full seeds without plant diseases and insect pests for later use, wherein the purity is over 80 percent.
S2, soaking seeds: soaking the seeds obtained in the step S1 in warm water of 35-45 ℃, continuously stirring, stopping stirring when the water temperature is reduced to 25 ℃, soaking for 3 days, changing water once a day in the soaking period, and repeating the operation each time.
S3, seed disinfection treatment: draining water from the seeds soaked in the S2, putting the seeds into 0.4-0.6% potassium permanganate solution for disinfection for 2 hours, taking out the seeds after disinfection, and repeatedly washing the seeds with a large amount of clear water until the seeds are not attached with the potassium permanganate light pink solution any more.
S4, river sand treatment: and (3) putting the river sand into 0.4-0.6% potassium permanganate solution for disinfection for 25-35min, and repeatedly leaching the river sand with clear water until the potassium permanganate solution is not attached to the river sand any more.
S5, mixing seed sand: and (3) uniformly mixing the seeds treated by the S3 and the river sand treated by the S4 according to the volume ratio of the seeds to the river sand being 1.
S6, temperature change treatment: placing the seed sand in an environment of 10-15 ℃ for 28-32 days, turning over once every 5 days, replenishing water in time, and keeping the water content of the seed sand at 45-55%;
placing the seed sand in an environment of 0-5 deg.C for 95-105 days, controlling the water content of the seed sand at 45-55%, and turning over once every 19-21 days;
finally, placing the seed sand in an environment of 20-25 ℃, controlling the water content of the seed sand to be 40-50%, turning over once every day, and when 30-40% of seeds germinate;
s7, hormone treatment: uniformly spraying 110-130mg/L double-Geer (GGR) seed sand until the seed sand is thoroughly poured, and covering a layer of air-permeable bag for seed sealing for 1-2h;
s8, greenhouse environment control and nutrition pot substrate disinfection: controlling the temperature of a greenhouse at 10-25 ℃, controlling the humidity at 30-60%, spraying 0.4-0.6% potassium permanganate solution on the nutrition pot substrate for disinfection, and airing for 3-5 days;
s9, sowing: irrigating the nutrition pot matrix in the greenhouse with clear water one day in advance, sowing 3-4 seeds treated by S7 in each nutrition pot with the depth of 1-1.5cm, covering soil and slightly pressing;
s10, managing after broadcasting: after sowing, before the seedlings come out of the soil, the nutrition pots are sprayed with clear water once in the morning and at night every day, after the seedlings grow out, watering is carried out according to soil moisture content, a shading net is covered, the light transmittance of the shading net is 30% -50%, hardening is carried out when 3-4 true leaves grow out from the seedlings, an arched shed is pried up for 3-5 small seams for ventilation for 2-3 days in the evening, if the seedlings do not have abnormal reaction, the ventilation openings are gradually enlarged, weeding is carried out regularly, and no weed is ensured.
S11, transplanting: and (4) watering the nutrition pot thoroughly one day before transplanting, and removing the nutrition pot during transplanting to transplant the nursery stock.
In a specific embodiment of the present invention, the temperature of the warm water used for S2 is 40 ℃.
In a specific embodiment of the invention, the S6 seed sand is placed in an environment of 10-15 ℃ for 30 days and then in an environment of 0-5 ℃ for 100 days.
In a specific embodiment of the present invention, the concentration of bis-gill (GGR) used in S7 is 120mg/L.
In a specific embodiment of the invention, the nutrition pot matrix in S8 is formed by mixing garden soil, river sand and decomposed sheep manure in a volume ratio of 4.
In a specific embodiment of the invention, the breathable bags in S5 and S7 are both breathable woven bags.
In a specific embodiment of the invention, the row spacing and the plant spacing of the seedlings transplanted by the S11 are 60cm-80cm, and the depth is 35cm-45cm.
In a specific embodiment of the invention, when S11 is transplanted, the nursery stock is righted, covered with soil and compacted, and watered thoroughly, and the transplanting time is selected in a cloudy day.
By adopting variable-temperature germination acceleration, namely, firstly carrying out medium-temperature and then carrying out high-temperature treatment, and utilizing the modes of nutrition pot sowing and hormone treatment, the germination rate and the emergence rate of lonicera caerulea can be obviously improved, the germination rate reaches more than 85%, the emergence rate reaches more than 90%, the survival rate of nursery stocks reaches more than 90%, and finally the purpose of improving the yield of lonicera caerulea is achieved. In addition, the lonicera caerulea cultivated in the nutrition pot is convenient to transport, the transplantation is not limited by time, the cultivation time and space of the lonicera caerulea are greatly widened, the market demand is further met, and the method is suitable for popularization and application.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It should be apparent that the described embodiments are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The specific implementation mode of the high-efficiency seedling raising technology for lonicera caerulea provided by the invention is as follows:
s1, seed harvesting and pretreatment, generally, firstly, harvesting mature fruits, and mashing the fruits in a container, wherein the strength is not too high in the mashing process, so that seeds are prevented from being damaged. Then repeatedly rinsing with water until the seeds are separated from the peel and the pulp, and then drying the seeds in the shade in a cool and ventilated place, wherein the seeds need to be frequently turned over to avoid mildewing. And finally, selecting plump seeds without diseases and insect pests for later use, wherein the cleanliness of the selected seeds is over 80 percent.
S2, soaking the seeds, soaking the selected seeds in warm water of 35-45 ℃, continuously stirring, stopping stirring when the water temperature is reduced to 25 ℃, soaking for 3 days, changing water once a day, and repeating the operation each time, namely, changing the water into the warm water of 35-45 ℃ when the water is changed, continuously stirring, stopping stirring when the water temperature is reduced to 25 ℃, and continuously soaking after the seeds are naturally cooled.
And S3, performing seed disinfection treatment, namely draining the soaked seeds, putting the seeds into 0.4-0.6% potassium permanganate solution for disinfection for 2 hours, taking out the seeds after disinfection, and repeatedly washing the seeds with a large amount of clear water until the potassium permanganate solution is not attached to the seeds any more.
And S4, treating the river sand, namely putting the river sand into a 0.4-0.6% potassium permanganate solution for disinfection for 25-35min, and repeatedly leaching the river sand with clear water until the river sand is not attached with the potassium permanganate light pink solution.
S5, mixing the seed sand treated by the S3 with the river sand treated by the S4 according to the volume ratio of the seed to the river sand of 1. In a specific implementation process, according to the experience of a technician, the seed sand can be held in a holding and loosening state, and of course, a specific method for controlling the water content can be thought of by the technician in the field, and is not described in detail herein. After the water content is controlled, the seed sand is filled into a breathable bag for storage, and in a specific embodiment, the breathable bag can be a breathable woven bag.
S6, performing temperature change treatment, namely placing the seed sand in an environment with the temperature of 10-15 ℃ for 28-32 days, turning over the seed sand once every 5 days, and supplementing water in time to keep the water content of the seed sand at 45-55%; then placing the seed sand in an environment of 0-5 deg.C for 95-105 days, controlling the water content of the seed sand to be 45-55%, turning over once every 19-21 days to prevent mildew, and treating for 95-105 days; then placing the seed sand in an environment of 20-25 ℃, controlling the water content of the seed sand to be 40-50%, turning over once every day, and when 30-40% of seeds germinate, the seed sand is obtained.
S7, hormone treatment, namely uniformly spraying 110-130mg/L double Gille (GGR) seed sand until the seed sand is poured through, covering a layer of breathable bag for seed sealing for 1-2 hours, so as to be beneficial to exerting the drug effect, and also adopting a breathable woven bag.
S8, greenhouse environment control and nutrition pot substrate disinfection, wherein the temperature of a greenhouse is controlled to be 10-25 ℃, the humidity is controlled to be 30-60%, the nutrition pot substrate is sprayed with 0.4-0.6% potassium permanganate solution for disinfection, and the nutrition pot substrate is aired for 3-5 days. In a specific embodiment, the nutrition pot matrix can be prepared by mixing garden soil, river sand and decomposed sheep manure in a volume ratio of 4. The nutrition pot is generally selected to be 9cm high and 10cm in diameter.
S9, sowing, wherein the nutrient pot substrate in the greenhouse is irrigated with clear water one day before sowing. The next day, 3-4 seeds treated by S7 are sown in each nutrition pot with the depth of 1-1.5cm, covered with soil and lightly pressed.
And S10, managing after sowing, and spraying the clean water to the nutrition bowls once every morning and evening before the seedlings come out of the soil after sowing. The nutrition pot can be sprayed by a water spray gun or a water sweeping kettle, and cannot be watered by overlarge water so as to prevent seeds from being washed out, and the seedlings are watered again according to the soil moisture content after growing. In order to prevent sun burn caused by direct sunlight, the seedlings are covered with a shading net, wherein the light transmittance of the shading net is preferably 30% -50%. Hardening the seedlings when 3-4 true leaves grow out from the seedlings, prying up the arched shed for 3-5 small seams to ventilate for 2-3 days in the evening, gradually enlarging ventilation openings if the seedlings have no abnormal reaction, and periodically and manually weeding to ensure no weeds.
S11, transplanting, and watering the nutrition pot thoroughly one day before transplanting. And (4) removing the nutrition pot during transplanting, and transplanting the nursery stock. In a specific embodiment, the row spacing and the plant spacing of the transplanted nursery stocks are 60cm-80cm, and the depth is about 40cm, so that the better growth of the nursery stocks can be ensured. Meanwhile, during transplanting, the nursery stock is preferably straightened, covered with soil and compacted, and watered thoroughly, and the transplanting time is selected in a cloudy day, so that transpiration of the nursery stock can be reduced, the seedling revival is facilitated, and the survival rate of the nursery stock is further improved.
According to the high-efficiency seedling raising technology for the lonicera caerulea provided by the invention, under different treatment conditions, the germination rate and the emergence rate of seeds are slightly different, and comparative analysis is carried out through a plurality of typical specific examples selected from a plurality of tests.
Example 1
Seed harvesting and pretreatment: harvesting and mashing mature fruits, repeatedly rinsing with water to separate seeds from peels and pulps, drying the seeds in the shade, and selecting full seeds without plant diseases and insect pests for later use, wherein the purity is over 80 percent.
Seed soaking: soaking the seeds obtained in the last step in warm water of 35 ℃, continuously stirring, stopping stirring when the water temperature is reduced to 25 ℃, soaking for 3 days, changing water once a day, and repeating the operation each time.
Seed disinfection treatment: draining water from the soaked seeds, putting the seeds into 0.4-0.6% potassium permanganate solution for disinfection for 2h, taking out the seeds after disinfection, and repeatedly washing the seeds with a large amount of clear water until the seeds are not attached with the potassium permanganate light pink solution any more.
Treating river sand: and (3) putting the river sand into 0.4-0.6% potassium permanganate solution for disinfection for 25-35min, and repeatedly leaching the river sand with clear water until the potassium permanganate solution is not attached to the river sand any more.
Seed sand mixing: uniformly mixing the disinfected seeds and river sand according to a volume ratio of the seeds to the river sand of 1.
Temperature change treatment: placing the seed sand in an environment of 10-15 ℃ for 28 days, turning over once every 5 days, and replenishing water in time to keep the water content of the seed sand at 45-55%; placing the seed sand in an environment of 0-5 deg.C for 95 days, controlling the water content of the seed sand at 45-55%, and turning over once every 19-21 days; finally, the seed sand is placed in an environment with the temperature of 20-25 ℃, the water content of the seed sand is controlled to be 40-50%, the seed sand is turned over once every day, and when 30-40% of seeds germinate, the seed sand can be obtained.
Taking a proper amount of seeds subjected to temperature change treatment, respectively placing the seeds in a plurality of culture dishes, adding filter paper, pouring tap water to soak the filter paper, preferably to prevent the occurrence of visible water, placing 100 seeds in each culture dish in an environment at the temperature of 20-25 ℃, adding water every day to keep the filter paper moist, timely cleaning out the mildewed seeds, counting the germination quantity when the seeds do not germinate for 3 consecutive days, and calculating the germination rate. (Germination test 1)
Hormone treatment: uniformly spraying the seed sand subjected to temperature change treatment by adopting 110mg/L double Gill (GGR) until the seed sand is poured through, and covering a layer of breathable bag for seed sealing for 1-2h.
Greenhouse environment control and nutrition pot substrate disinfection: controlling the temperature of the greenhouse at 10-25 deg.C, controlling the humidity at 30-60%, spraying 0.4-0.6% potassium permanganate solution on the nutrition pot substrate for sterilization, and air drying for 3-5 days.
Sowing: irrigating the nutrition pot matrix in the greenhouse with clear water one day in advance, sowing 3-4 seeds treated by the hormone in each nutrition pot with the depth of 1-1.5cm, covering soil and slightly pressing.
Managing after broadcasting: after sowing, before the seedlings come out of the soil, the nutrition pots are sprayed with clear water once in the morning and at night every day, after the seedlings come out, watering is carried out according to soil moisture content, the number of seedlings is counted, and the rate of emergence is calculated. (emergence test 1)
Covering the seedlings with a shading net, wherein the light transmittance of the shading net is 30% -50%, hardening the seedlings when 3-4 true leaves grow out from the seedlings, prying up an arched shed for 3-5 small gaps to ventilate for 2-3 days in the evening, gradually increasing the ventilation openings if the seedlings have no abnormal reaction, regularly weeding to ensure no weed, counting survival number 20-30 days after emergence of seedlings, and calculating survival rate. (survival test 1)
Transplanting: and (4) watering the nutrition pot thoroughly one day before transplanting, and removing the nutrition pot during transplanting to transplant the nursery stock.
Example 2
Seed harvesting and pretreatment: collecting mature fruits, mashing, repeatedly rinsing with water to separate seeds from pericarp and pulp, drying in the shade, and selecting plump seeds without diseases and insect pests for use, wherein the purity is more than 80%.
Seed soaking: soaking the seeds obtained in the last step in warm water of 40 ℃, continuously stirring, stopping stirring when the water temperature is reduced to 25 ℃, soaking for 3 days, changing water once a day, and repeating the operation each time.
Seed disinfection treatment: draining off water from the soaked seeds, putting the seeds into 0.4-0.6% potassium permanganate solution for disinfection for 2h, taking out the seeds after disinfection, and repeatedly washing the seeds with a large amount of clear water until the seeds are not attached with the potassium permanganate light pink solution.
Treating river sand: and (3) putting the river sand into 0.4-0.6% potassium permanganate solution for disinfection for 25-35min, and repeatedly leaching the river sand with clear water until the potassium permanganate solution is not attached to the river sand any more.
Seed sand mixing: uniformly mixing the disinfected seeds and river sand according to the volume ratio of the seeds to the river sand being 1.
Temperature change treatment: placing the seed sand in an environment of 10-15 ℃ for 30 days, turning over once every 5 days, supplementing water in time, and keeping the water content of the seed sand at 45-55%; placing the seed sand in an environment of 0-5 deg.C for 100 days, controlling the water content of the seed sand at 45-55%, and turning over once every 19-21 days; finally, the seed sand is placed in an environment with the temperature of 20-25 ℃, the water content of the seed sand is controlled to be 40-50%, the seed sand is turned over once every day, and when 30-40% of seeds germinate, the seed sand can be obtained.
Taking a proper amount of seeds subjected to temperature change treatment, respectively placing the seeds in a plurality of culture dishes, adding filter paper, pouring tap water to soak the filter paper, preferably to prevent the occurrence of visible water, placing 100 seeds in each culture dish in an environment at the temperature of 20-25 ℃, adding water every day to keep the filter paper moist, timely cleaning out the mildewed seeds, counting the germination quantity when the seeds do not germinate for 3 consecutive days, and calculating the germination rate. (Germination test 2)
Hormone treatment: uniformly spraying 120mg/L double Gill (GGR) with seed sand subjected to temperature change treatment until the seed sand is thoroughly poured, and covering with a layer of air permeable bag for seed sealing for 1-2h.
Greenhouse environment control and nutrition pot substrate disinfection: controlling the temperature of a greenhouse at 10-25 ℃, controlling the humidity at 30-60%, spraying 0.4-0.6% potassium permanganate solution on the nutrition pot substrate for sterilization, and airing for 3-5 days.
Sowing: irrigating the nutrition pot matrix in the greenhouse with clear water one day in advance, sowing 3-4 seeds treated by the hormone in each nutrition pot with the depth of 1-1.5cm, covering soil and slightly pressing.
Managing after broadcasting: after sowing, before the seedlings come out of the soil, the seedlings are sprayed with clear water once in the morning and evening each day, after the seedlings come out, watering is carried out according to soil moisture content, the number of seedlings is counted, and the rate of emergence is calculated. (emergence test 2)
Covering the seedlings with a shading net, wherein the light transmittance of the shading net is 30% -50%, hardening the seedlings when 3-4 true leaves grow out from the seedlings, prying up an arched shed for 3-5 small gaps to ventilate for 2-3 days in the evening, gradually increasing the ventilation openings if the seedlings have no abnormal reaction, regularly weeding to ensure no weed, counting survival number 20-30 days after emergence of seedlings, and calculating survival rate. (survival test 2)
Transplanting: and (4) watering the nutrition pot thoroughly one day before transplanting, and removing the nutrition pot during transplanting to transplant the nursery stock.
Example 3
Seed harvesting and pretreatment: collecting mature fruits, mashing, repeatedly rinsing with water to separate seeds from pericarp and pulp, drying in the shade, and selecting plump seeds without diseases and insect pests for use, wherein the purity is more than 80%.
Seed soaking: soaking the seeds obtained in the last step in 45 ℃ warm water, continuously stirring, stopping stirring when the water temperature is reduced to 25 ℃, soaking for 3 days, changing water once a day, and repeating the operation each time.
Seed disinfection treatment: draining water from the soaked seeds, putting the seeds into 0.4-0.6% potassium permanganate solution for disinfection for 2h, taking out the seeds after disinfection, and repeatedly washing the seeds with a large amount of clear water until the seeds are not attached with the potassium permanganate light pink solution any more.
Treating river sand: and (3) putting the river sand into 0.4-0.6% potassium permanganate solution for disinfection for 25-35min, and repeatedly leaching the river sand with clear water until the potassium permanganate solution is not attached to the river sand any more.
Seed sand mixing: uniformly mixing the disinfected seeds and river sand according to the volume ratio of the seeds to the river sand being 1.
Temperature change treatment: placing the seed sand in an environment of 10-15 ℃ for 32 days, turning over once every 5 days, supplementing water in time, and keeping the water content of the seed sand at 45-55%; placing the seed sand in an environment of 0-5 deg.C for 105 days, controlling the water content of the seed sand at 45-55%, and turning over once every 19-21 days; finally, the seed sand is placed in an environment with the temperature of 20-25 ℃, the water content of the seed sand is controlled to be 40-50%, the seed sand is turned over once every day, and when 30-40% of seeds germinate, the seed sand can be obtained.
Taking a proper amount of seeds subjected to temperature change treatment, respectively placing the seeds in a plurality of culture dishes, adding filter paper, pouring tap water to soak the filter paper, preferably, no visible water is available, placing 100 seeds in each culture dish in an environment at 20-25 ℃, adding water every day to keep the filter paper moist, timely cleaning out the mildewed seeds, counting the germination number when the seeds do not germinate for 3 consecutive days, and calculating the germination rate. (Germination test 3)
Hormone treatment: uniformly spraying 130mg/L double Gill (GGR) with seed sand subjected to temperature change treatment until the seed sand is completely poured, and covering with a layer of air permeable bag for seed sealing for 1-2h.
Greenhouse environment control and nutrition pot substrate disinfection: controlling the temperature of the greenhouse at 10-25 deg.C, controlling the humidity at 30-60%, spraying 0.4-0.6% potassium permanganate solution on the nutrition pot substrate for sterilization, and air drying for 3-5 days.
Sowing: irrigating the nutrition pot matrix in the greenhouse with clear water one day in advance, sowing 3-4 seeds treated by the hormone in each nutrition pot with the depth of 1-1.5cm, covering soil and slightly pressing.
Managing after broadcasting: after sowing, before the seedlings come out of the soil, the nutrition pots are sprayed with clear water once in the morning and at night every day, after the seedlings come out, watering is carried out according to soil moisture content, the number of seedlings is counted, and the rate of emergence is calculated. (emergence test 3)
Covering the seedlings with a shading net, wherein the light transmittance of the shading net is 30% -50%, hardening the seedlings when 3-4 true leaves grow out from the seedlings, prying up an arched shed for 3-5 small gaps to ventilate for 2-3 days in the evening, gradually increasing the ventilation openings if the seedlings have no abnormal reaction, regularly weeding to ensure no weed, counting survival number 20-30 days after emergence of seedlings, and calculating survival rate. (survival test 3)
Transplanting: and (4) watering the nutrition pot thoroughly one day before transplanting, and removing the nutrition pot during transplanting to transplant the nursery stock.
Summary of test data
1. The test adopts a data summarization mode, and the germination rate, the emergence rate and the survival rate obtained by calculation after data is observed and recorded in the germination tests 1, 2 and 3, the emergence tests 1, 2 and 3 and the survival tests 1, 2 and 3 are compared and analyzed with the related data of a common germination accelerating and field sowing mode.
Wherein, germination rate = number of seeds germination/total number of seeds;
emergence rate = number of seedlings/number of seeds sowed;
survival = number of surviving/number of emerged seedlings.
2. Data logging results
TABLE 1 comparison of germination rates of seeds under different treatment conditions
Figure BDA0003914633380000141
As can be seen from table 1, the germination rate of the seeds can be significantly improved by adopting a variable-temperature germination accelerating mode, and the germination rate of the seeds can reach more than 85% by applying the technology of the present invention. In addition, under the conditions of the germination test 2, the germination rate of the seeds is higher than that of the germination test 1 and the germination test 3, so the following conditions are adopted as relatively optimal choices: when the seeds are soaked, warm water is adopted, and the temperature is 40 ℃; the temperature-changing treatment comprises placing the seed sand in 10-15 deg.C environment for 30 days, and then in 0-5 deg.C environment for 100 days.
TABLE 2 comparison of emergence rates of seeds under different treatment conditions
Figure BDA0003914633380000142
As can be seen from Table 2, the emergence rate of lonicera caerulea can be significantly improved by using the modes of nutrition pot sowing and hormone treatment, and the emergence rate of the seeds can reach more than 90% by applying the technology of the invention. Moreover, under the condition of the emergence test 2, the emergence rate of the seeds is higher than that of the emergence test 1 and the emergence test 3, so the following conditions are adopted as relatively optimal choices: the concentration of double Gill (GGR) used for the treatment of seed hormones was 120mg/L.
TABLE 3 comparison of survival rates of seedlings under different treatment conditions
Figure BDA0003914633380000151
As can be seen from Table 3, the survival rate of the seedlings can reach more than 90% by applying the technology of the present invention.
According to the embodiment of the invention, the germination rate and the emergence rate of lonicera caerulea can be obviously improved by adopting variable-temperature germination acceleration on seeds, sowing in a nutrition pot and hormone treatment, and the tests show that the germination rate of the seeds can reach more than 85%, the emergence rate can reach more than 90%, the survival rate of seedlings can reach more than 90%, and finally the aim of improving the yield of the lonicera caerulea is achieved. In addition, the lonicera caerulea cultivated in the nutrition pot is convenient to transport, the transplantation is not limited by time, the cultivation time and space of the lonicera caerulea are greatly widened, the market demand is further met, and the method is suitable for popularization and application.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.

Claims (8)

1. An efficient seedling raising technology for lonicera caerulea is characterized by comprising the following steps:
s1, seed harvesting and pretreatment: harvesting and mashing mature fruits, repeatedly rinsing with water to separate seeds from peels and pulps, drying the seeds in the shade, and selecting full seeds without plant diseases and insect pests for later use, wherein the purity is over 80 percent;
s2, soaking seeds: soaking the seeds obtained in the step S1 in warm water of 35-45 ℃, continuously stirring, stopping stirring when the water temperature is reduced to 25 ℃, soaking for 3 days, changing water once a day in the soaking period, and repeating the operation each time.
S3, seed disinfection treatment: draining the seeds soaked in the S2, putting the seeds into 0.4-0.6% potassium permanganate solution for disinfection for 2 hours, taking out the seeds after disinfection, and repeatedly washing the seeds with a large amount of clear water until the seeds are not attached with the potassium permanganate light pink solution any more;
s4, river sand treatment: placing river sand into 0.4-0.6% potassium permanganate solution for disinfection for 25-35min, and repeatedly leaching with clear water until no potassium permanganate light pink solution adheres to the river sand;
s5, mixing seed sand: uniformly mixing the seeds treated by the S3 and the river sand treated by the S4 according to the volume ratio of the seeds to the river sand being 1;
s6, temperature change treatment: placing the seed sand in an environment of 10-15 ℃ for 28-32 days, turning over once every 5 days, replenishing water in time, and keeping the water content of the seed sand at 45-55%;
placing the seed sand in an environment of 0-5 deg.C for 95-105 days, controlling the water content of the seed sand at 45-55%, and turning over once every 19-21 days;
finally, placing the seed sand in an environment of 20-25 ℃, controlling the water content of the seed sand to be 40-50%, turning over once every day, and when 30-40% of seeds germinate;
s7, hormone treatment: uniformly spraying 110-130mg/L double Gill (GGR) with seed sand until the seed sand is poured thoroughly, and covering with a layer of air-permeable bag for seed sealing for 1-2h;
s8, greenhouse environment control and nutrition pot substrate disinfection: controlling the temperature and humidity of a greenhouse at 10-25 ℃, controlling the humidity at 30-60%, spraying a 0.4-0.6% potassium permanganate solution on a nutrition pot substrate for disinfection, and airing for 3-5 days;
s9, sowing: irrigating the nutrition pot matrix in the greenhouse with clear water one day in advance, sowing 3-4 seeds treated by S7 in each nutrition pot with the depth of 1-1.5cm, covering soil and slightly pressing;
s10, managing after broadcasting: after sowing, before the seedlings come out of the soil, the nutrition pots are sprayed with clear water once in the morning and at night every day, after the seedlings grow out, watering is carried out according to soil moisture content, a shading net is covered, the light transmittance of the shading net is 30% -50%, hardening is carried out when 3-4 true leaves grow out from the seedlings, an arched shed is pried up for 3-5 small seams for ventilation for 2-3 days in the evening, if the seedlings do not have abnormal reaction, the ventilation openings are gradually enlarged, weeding is carried out regularly, and no weed is ensured;
s11, transplanting: and (4) watering the nutrition pot thoroughly one day before transplanting, and removing the nutrition pot during transplanting to transplant the nursery stock.
2. The high-efficiency seedling raising technology of lonicera caerulea according to claim 1, wherein the temperature of warm water adopted in S2 is 40 ℃.
3. The high-efficiency seedling raising technology of lonicera caerulea according to claim 1, wherein S6 seed sand is placed in an environment of 10-15 ℃ for 30 days and then in an environment of 0-5 ℃ for 100 days.
4. The high-efficiency seedling raising technology of lonicera caerulea according to claim 1, wherein the concentration of digger (GGR) adopted in S7 is 120mg/L.
5. The efficient lonicera caerulea seedling raising technology according to claim 1, wherein the nutrition pot matrix in S8 is formed by mixing garden soil, river sand and decomposed sheep manure in a volume ratio of 4.
6. The high-efficiency seedling raising technology of lonicera caerulea according to claim 1, wherein the breathable bags in S5 and S7 are breathable woven bags.
7. The efficient lonicera caerulea seedling raising technology of claim 1, wherein the row spacing and the plant spacing of seedlings transplanted in S11 are 60cm-80cm, and the depth is 35cm-45cm.
8. The high-efficiency indigo honeysuckle seedling growing technology of claim 1, wherein during S11 transplanting, nursery stocks are righted, covered with soil and compacted, and watered thoroughly, and transplanting time is selected in a cloudy day.
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CN105210625A (en) * 2015-10-09 2016-01-06 陕西省西安植物园 A kind ofly pass through a moon method for Seeds pression nursery
CN111937525A (en) * 2020-08-11 2020-11-17 湖南农佳好伙计农业科技发展有限公司 Paris polyphylla seed sand storage, temperature change and medicament treatment germination accelerating method
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