CN107889576A - A kind of Scaevola aemula seedling quick efficient breeding method - Google Patents
A kind of Scaevola aemula seedling quick efficient breeding method Download PDFInfo
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- CN107889576A CN107889576A CN201711281828.9A CN201711281828A CN107889576A CN 107889576 A CN107889576 A CN 107889576A CN 201711281828 A CN201711281828 A CN 201711281828A CN 107889576 A CN107889576 A CN 107889576A
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- Prior art keywords
- seed
- scaevola
- scaevola aemula
- aemula
- seedling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The invention discloses a kind of Scaevola aemula seedling quick efficient breeding method.It is that Scaevola aemula (Scaevola sericea Vahl) seed is soaked into 0~48h in the 0~400mg/L gibberellin aqueous solution, Scaevola aemula seed after immersion is seeded in sand bed, sand bed humid control is 60%~90%, the Germination Temperature of seed is 25 DEG C~30 DEG C, and seed is sprouted and grown until growing up to Scaevola aemula seedling.The present invention directly carries out rapidly and efficiently nursery with Scaevola aemula seed, the beginning sprout time for realizing Scaevola aemula seed shifts to an earlier date more than 12 days, improve seed and sprout bud ratio to more than 92%, seedling transplanting survival rate has exceeded 80.2%, and produces the high-quality Scaevola aemula seedling of neat and consistent.To meet the active demand of this important island plant seedling of Scaevola aemula and recovering protection technical support is provided with resources development and utilization.
Description
Technical field：
The invention belongs to breeding plant technical field, and in particular to a kind of Scaevola aemula seedling quick efficient breeding method.
Background technology：
Scaevola aemula (Scaevola sericea Vahl) is Goodeniaceae (Goodeniaceae) Scaevola aemula category
(Scaevola) upright or paving dissipates the mangrove associates of shrub.There are 11 category, 400 kind (Carolin et al.Flora in full section
of Australia,volume 35,Brunoniaceae,Goodeniaceae.Australian Government
Publishing Service, Canberra, Australian Capital Territory Australia, 1992), almost
All origin and existing species mostly concentrate on Australia, and only Scaevola aemula category is widely distributed in the whole world.Scaevola aemula category has 130
Kind, only 40 kinds being distributed in beyond Australia, tropical Island (Howarth et are sporadically distributed in mostly
al.Phylogenetics of the genus Scaevola(Goodeniaceae):implication for
dispersal patterns across the Pacific Basin and colonization of the Hawaiian
Islands.American Journal of Botany,2003,90:915-923.).South China is coastal 2 kinds, respectively
It is Scaevola aemula (Scaevola sericea Vahl) and Hainan Scaevola aemula (Scaevola hainanensis Hance), mainly
It is distributed in the ground such as Taiwan, Fujian, Guangdong, Guangxi and Hainan and opens on the cliff of spacious seashore sand ground or seashore (peaceful generation river etc., Guangxi
The investigation GUIHAIAs of island Mangrove resource, 1995,15:139-145), have and check winds and fix drifting sand, recover the heat degenerated
Have the function that with island ecosystem important, be one of dominant tree of tropical island plant.The pattern of Scaevola aemula is gorgeous, flower
Phase is longer, and branch type is graceful, therefore is often ornamental type plant.Scaevola aemula also has important medical value, and its blade contains abundant
The chemical compositions such as palmitic acid, leukotrienes, phytol, cumarin, diterpene, triterpene, glycocide, has antibacterial effect simultaneously to there is knife
Wound, animal bite, cataract, flaky skin, tinea, stomach trouble, improve eye congestion and swelling pain, contraception and enhancing sexual function etc. also have
Certain effect (Li Min, wait Scaevola aemula leaves main volatile chemical composition and bacteriostatic activity Chemical Engineering Technologies with exploitation, 2015,
44:10-14.)。
Due to the mankind conservation of nature is realized not enough, the reason such as is destroyed in habitat makes the natural resources of this kind of plant very
It is limited.Has information, the seed of Scaevola aemula platymiscium is very difficult to sprout under field conditions (factors), and generally use cuttage mode is come
Breed (Wrigley and Fagg, Australian Native Plants.W.Collins Pty Ltd:Sydney.)(1988)
.3rd Eds).We found in on-the-spot investigation on the island of Hainan Province Wenchang City Scaevola aemula be local group sociales or
Common Species, the plant of adult has mast-fruiting event, but the seed germination rate for being scattering into underground is very low, less than 10%, this also with money
Material is recorded consistent.
The content of the invention：
It is an object of the invention to provide the Scaevola aemula seedling that a kind of sprout time is short, seed germination rate is high is rapidly and efficiently numerous
Educate method.
The Scaevola aemula seedling quick efficient breeding method of the present invention, comprises the following steps：Collect ripe Scaevola aemula
(Scaevola sericea Vahl) fruit, the exocarp of fruit is removed after natural air drying, obtains Scaevola aemula (Scaevola
Sericea Vahl) seed, Scaevola aemula (Scaevola sericea Vahl) seed is soaked in the gibberellin aqueous solution, will
Scaevola aemula seed after immersion is seeded in sand bed, and seed is sprouted and grown until growing up to Scaevola aemula seedling.
The concentration of the described gibberellin aqueous solution is preferably 0~400mg/L.
The concentration of the described gibberellin aqueous solution is more preferably 100~400mg/L.
The concentration of the described gibberellin aqueous solution is more preferably 200~400mg/L.
Described soaks Scaevola aemula seed in the gibberellin aqueous solution, and its soak time is preferably 0~48h.Described
Soak time is more preferably 4~48h.Described soak time is more preferably 24~48h.
It is preferred that the relative humidity control of described sand bed, 60%~90%, the Germination Temperature of described seed is 25 DEG C
~30 DEG C.
Exocarp (direct method) can be removed or by nature by artificial single-handedly peel off by removing the exocarp of Scaevola aemula fruit
Air-dried Scaevola aemula fruit makes pericarp fully absorb water after softening through clear water immersion 30min or so, with hand to rub with the hands remove exocarp (
Connection).
Compared with prior art, its advantage is the present invention：
1st, the breeding of method of the Scaevola aemula seedling of system rapidly and efficiently is established first.
2nd, Scaevola aemula seed has been drawn the reason for natural germination rate is low and proposes corresponding solution.
3rd, rapidly and efficiently nursery is directly carried out with Scaevola aemula seed, the beginning sprout time for realizing Scaevola aemula seed shifts to an earlier date
More than 12 days, improve seed and sprout bud ratio to more than 92%, seedling transplanting survival rate has exceeded 80.2%, and produces neat one
The high-quality Scaevola aemula seedling caused.For meet the active demand of this important island plant seedling of Scaevola aemula and recover protection with
Resources development and utilization provides technical support.
Brief description of the drawings：
Fig. 1 is Scaevola aemula fresh fruit design sketch；
Fig. 2 is Scaevola aemula fruit natural air drying design sketch after 2 weeks；
Fig. 3 is that Scaevola aemula whole fruit the rotten design sketch that goes mouldy occurs in seeding process；
Fig. 4 is to remove exocarp design sketch indirectly after clear water soaks 30min；
Fig. 5 is the design sketch after Scaevola aemula seed is sprouted 50 days；
Fig. 6 is design sketch of the seedling replanting after 1 month.
Embodiment：
Following examples are that the present invention is further illustrated, rather than limitation of the present invention.
The reagent materials such as gibberellin, beaker, graduated cylinder used in seed treatment are commercially available, as shown in catalogue
Conventional method adapted, it is general for industry.All experiments have carried out repeating to test three times, result of the test will be put down three times
, final result of the test is obtained.
Embodiment 1：
The influence experiment sprouted for determining Scaevola aemula exocarp to seed, collects Scaevola aemula fruit in October, 2016 and enters
Row Seed Germination Test, is comprised the following steps that：
(1) collecting fruit：Collect ripe Scaevola aemula (Scaevola sericea Vahl) fruit (figure in October, 2016
1) it is locality, Hainan Province Sanya Xi Dao, natural air drying 2 weeks is with standby in laboratory.
(2) fruit is chosen：Take Scaevola aemula fruit (see Fig. 2) 100 of the natural air drying after 2 weeks.
(3) fruit removes exocarp processing：Scaevola aemula fruit is uniformly divided into two group 1~2 at random, the grass sea of one of which
Paulownia fruit directly removes its exocarp.Test group 1 is not remove the whole fruit of exocarp, and test group 2 is its loose richness of removal
The seed of nutritious exocarp.
(4) fruit soaks：Fruits and seedses do not carry out immersion treatment.
(5) seed is sprouted：Fruit and seed are seeded in the sand bed of moistening respectively and carry out seed sprouting incubation.Seed
Germination Temperature is 25 DEG C~30 DEG C.Appropriate clear water is sprayed daily, is ensured sand bed humidity between 60%~90% and is observed note
Record seed sprouting situation.The time is observed and recorded as 60 days.
Seed Germination Test is carried out using the above method, resulting the results are shown in Table 1：
The Scaevola aemula seed of table 1. sow 60 days after sprouting situation
As shown in Table 1, the seed of the whole fruit of test group 1 starts to sprout after sowing 32 days, the seed of test group 2
Start to sprout after sowing 22 days, seed germination rate is respectively 12.1% and 45.3%.Understand fruit except the going of test group 2
The seed of skin start sprout time than test group 1 be advanced by 10 days and seed germination rate is significantly higher than test group 1.Thus may be used
Know the exocarp of Scaevola aemula Scaevola aemula seed is sprouted it is inhibited, remove exocarp can alleviate this inhibitory action so as to
Make seed start sprout time to shift to an earlier date and improve seed germination rate.In the big portion of fruit of the visible test group 1 of Seed Germination
Distribute mould rotten (Fig. 3).Because Scaevola aemula exocarp contains abundant nutriment, easy mould growth and mouldy make kind
The rotten death of son, the seed of the test group 2 after exocarp is removed then without this phenomenon, it is test group 1 to illustrate that the mold of seed is rotted
One of the reason for seed germination rate is relatively low.
By embodiment 1, we know one of the reason for Scaevola aemula seed germination rate is low, and determine to remove outside Scaevola aemula
Pericarp plays the role of positive to seed sprout time and germination rate, but the beginning germination process for managing lower seed in this place is time-consuming longer
And germination rate is relatively low, only 45.3%.This reaches far away the requirement of efficient preferable seedling breeding, therefore also needs to enter this traveling one
The discussion of step.
Embodiment 2：
For determining whether clear water immersion different time has an impact to seed sprouting, natural air drying 2 is taken in October, 2016
Scaevola aemula fruit after week carries out Seed Germination Test, comprises the following steps that：
(1) fruit is chosen：Take Scaevola aemula (the Scaevola sericea Vahl) fruit of natural air drying after 2 weeks (see Fig. 2)
200.
(2) fruit goes exocarp to handle：Fruit is soaked in 30min or so in clear water, pericarp is fully absorbed water after softening,
The exocarp of Scaevola aemula can be removed clean (see Fig. 4) easily to rubbing with the hands with hand.
(3) fruit soaks：The seed for removing exocarp is got from clear water, 4 parts is divided into, 50 every part, divides
It Biao Ji not be 2~5, and be individually placed to soak 0h, 4h, 12h, 24h and 48h in fresh clear water.
(4) seed is sprouted：Seed is seeded in respectively in the sand bed of moistening and carries out seed sprouting incubation.The sprouting temperature of seed
Spend for 25 DEG C~30 DEG C.Appropriate clear water is sprayed daily, ensures that sand bed relative humidity between 60%~90% and observes and records
Seed sprouting situation.The time is observed and recorded as 60 days.
Seed Germination Test is carried out using the above method, resulting the results are shown in Table 2：
Seed after the Scaevola aemula of table 2. removal exocarp soaks the seed sprouting situation after different time in clear water
As shown in Table 2, directly it is seeded into without the seed of immersion in sand bed, its seed germination rate ratio is by immersion (4-
24h) much lower.The clear water immersion treatment 4h seed of test group 3 started to sprout at after planting 22 days, and soaked 12h and 24h
Test group 4 and test group 5 started to sprout at 20 days, the beginning sprout time than the seed of test group 3 is advanced by 2 days.Test group 3
~6 are respectively provided with higher seed germination rate after 60d is sowed, seed germination rate is respectively 83.5%, 85.4%, 87.6%,
87.4%.Understand to be remarkably improved Scaevola aemula seed germination rate with clear water immersion Scaevola aemula seed 4~48h processing, and when immersion
Time can make seed start sprout time to shift to an earlier date when reaching more than 12h.Show that plantation immersion can remove some Inhibiting germinations
Chemical substance, seed is promoted to sprout.Soak time is longer (within 24-48h), and seed germination rate is higher.
The germination rate of seed can be effectively improved up to more than 87.6% by immersion treatment, because the immersion of seed
Seed can be made to carry out quick imbitition and some chemical compositions for suppressing seed sprouting is discharged from seed.
Although having higher seed germination rate, seed start sprout time still take it is longer, in order to contract
The time required to short Seed Germination, also need further to inquire into the Germination characteristics of seed.
Embodiment 3：
For the influence for determining to sprout seed through various concentrations gibberellin immersion treatment, on October 10th, 2016, take
Scaevola aemula fruit of the natural air drying after 2 weeks carries out Seed Germination Test, comprises the following steps that：
(1) fruit is chosen：Take Scaevola aemula (the Scaevola sericea Vahl) fruit of natural air drying after 2 weeks (see Fig. 2)
200.
(2) fruit goes exocarp to handle：Scaevola aemula fruit is directly removed into its exocarp and is uniformly divided into 4 parts, every part 50
It is individual, it is respectively labeled as test group 7~10.
(3) fruit soaks：By the seed of test group 7~10 be soaked in respectively concentration for 0,100mg/L, 200mg/L,
24h in the 400mg/L gibberellin aqueous solution.
(4) seed is sprouted：Seed after immersion is seeded in the sand bed of moistening respectively and carries out seed sprouting incubation.Seed
Germination Temperature be 25 DEG C~30 DEG C.Appropriate clear water is sprayed daily, ensures sand bed relative humidity between 60%~90% simultaneously
Observe and record seed sprouting situation.The time is observed and recorded as 60 days.
Seed Germination Test is carried out using the above method, resulting the results are shown in Table 3：
The influence that the various concentrations gibberellin of table 3. immersion 24h processing Scaevola aemula seeds are sprouted to it
As shown in Table 3, the Scaevola aemula seed soaked through 200-400mg/L gibberellin starts to sprout in 18d, and its elsewhere
The Scaevola aemula seed of reason group is to start to sprout at 20 days, illustrates that Scaevola aemula seed sprout time can carry after gibberellin soaks
Sprout within first 2 days.After planting 60d statistics finds that the seed germination rate highest through 200mg/L gibberellin immersion treatment groups is reachable
92.3%, the seed germination rate through 400mg/L gibberellin immersion treatment groups is also 92.2%.But the seed of other treatment groups is sprouted
Rate is not also low, reaches more than 89.6%.It follows that the germination rate of Scaevola aemula seed is in various concentrations gibberellin immersion treatment
Test group between be respectively provided with higher germination rate, but the immersion of high concentration gibberellin can make that Scaevola aemula seed starts to sprout when
Between in advance, therefore 200mg/L gibberellin immersion 24h be Scaevola aemula seed sprout optimal immersion treatment.
Embodiment 4：
Tested for the transplantation of seedlings of Scaevola aemula kind, specific method is as follows：
By the test group 9 of embodiment 3 handle obtained well-grown Scaevola aemula seedling move to (see Fig. 5) appropriate sunshade and
Tame 1 week in ventilated greenhouse, then by the seedling after domestication be carefully transplanted to equipped with matrix (matrix be by peat soil,
Yellow mud and vermiculite by volume 1:1:1 well mixed forms) black Seedling bag (12cm × 14cm) in a bag seedling is made.Move
Enough root waters are poured during cultivation.
Scaevola aemula kind transplantation of seedlings is carried out using the above method, it is as a result as follows：
Survival rate is 86.1% during 15 days after transplanting of Scaevola aemula seedling, to 30d after when survival rate be but 80.2%.Children
Seedling is because a small amount of plant is rotted the reason for the survival rate after transplanting is cultivated 15 days is reduced, and is primarily determined that to pour
Caused by dilutional hyponatremia, reduce after this irrigation amount to make soil conservation moisten but tack-free humidity (relative humidity 60% is left
It is right) find that Scaevola aemula seedling does not occur rotten phenomenon and well-grown again (see Fig. 6).
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail with reference to preferred embodiment to the present invention, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent substitution, without departing from the essence of technical solution of the present invention
And scope.
Claims (9)
1. a kind of Scaevola aemula seedling quick efficient breeding method, it is characterised in that collect the Scaevola aemula (Scaevola of maturation
Sericea Vahl) fruit, the exocarp of fruit is removed after natural air drying, Scaevola aemula seed is obtained, by Scaevola aemula seed in red
Soaked in the mycin aqueous solution, the Scaevola aemula seed after immersion is seeded in sand bed, seed is sprouted and grown until growing up to Cao Hai
Paulownia seedling.
2. Scaevola aemula seedling quick efficient breeding method according to claim 1, it is characterised in that described gibberellin water
The concentration of solution is 0~400mg/L.
3. Scaevola aemula seedling quick efficient breeding method according to claim 2, it is characterised in that described gibberellin water
The concentration of solution is 100~400mg/L.
4. Scaevola aemula seedling quick efficient breeding method according to claim 3, it is characterised in that described gibberellin water
The concentration of solution is 200~400mg/L.
5. Scaevola aemula seedling quick efficient breeding method according to claim 1 or 2, it is characterised in that described by grass
Pittosporum tobira seed soaks in the gibberellin aqueous solution, and its soak time is 0~48h.
6. Scaevola aemula seedling quick efficient breeding method according to claim 5, it is characterised in that described soak time
For 4~48h.
7. Scaevola aemula seedling quick efficient breeding method according to claim 6, it is characterised in that described soak time
For 24~48h.
8. Scaevola aemula seedling quick efficient breeding method according to claim 1 or 2, it is characterised in that described sand bed
Relative humidity control 60%~90%, the Germination Temperature of described seed is 25 DEG C~30 DEG C.
9. Scaevola aemula seedling quick efficient breeding method according to claim 1 or 2, it is characterised in that described removal
The exocarp of fruit is to remove exocarp by artificial single-handedly stripping or make the Scaevola aemula fruit of natural air drying through clear water immersion
Pericarp is fully absorbed water after softening, and exocarp is removed to rubbing with the hands with hand.
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| CN201711281828.9A CN107889576B (en) | 2017-12-07 | 2017-12-07 | Method for quickly and efficiently breeding pittosporum tobira seedlings |
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| CN201711281828.9A CN107889576B (en) | 2017-12-07 | 2017-12-07 | Method for quickly and efficiently breeding pittosporum tobira seedlings |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108739320A (en) * | 2018-05-24 | 2018-11-06 | 中国科学院华南植物园 | A kind of wind resistance paulownia seed is sprouted and the method for nursery |
| CN113080051A (en) * | 2021-03-26 | 2021-07-09 | 厦门海石生态环境股份有限公司 | Method for promoting recovery of shallow sea seaweed bed |
| CN114097511A (en) * | 2021-11-12 | 2022-03-01 | 海南大学 | Method for rapidly greening tropical and subtropical coastal sandy land |
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| WO2007121518A1 (en) * | 2006-04-21 | 2007-11-01 | The State Of Western Australia Through Its Department Of Agriculture | Improved plant culture methods using a modified auxin treatment step |
| CN103004323A (en) * | 2013-01-16 | 2013-04-03 | 广西壮族自治区林业科学研究院 | Method for promoting germination of cinnamon seed |
| CN106879275A (en) * | 2017-01-10 | 2017-06-23 | 中国科学院华南植物园 | A kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007121518A1 (en) * | 2006-04-21 | 2007-11-01 | The State Of Western Australia Through Its Department Of Agriculture | Improved plant culture methods using a modified auxin treatment step |
| CN103004323A (en) * | 2013-01-16 | 2013-04-03 | 广西壮族自治区林业科学研究院 | Method for promoting germination of cinnamon seed |
| CN106879275A (en) * | 2017-01-10 | 2017-06-23 | 中国科学院华南植物园 | A kind of quick method for effectively improving Hainan Chinese scholartree percentage of seedgermination |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108739320A (en) * | 2018-05-24 | 2018-11-06 | 中国科学院华南植物园 | A kind of wind resistance paulownia seed is sprouted and the method for nursery |
| CN113080051A (en) * | 2021-03-26 | 2021-07-09 | 厦门海石生态环境股份有限公司 | Method for promoting recovery of shallow sea seaweed bed |
| CN113080051B (en) * | 2021-03-26 | 2023-10-20 | 厦门海石生态环境股份有限公司 | Method for promoting recovery of shallow sea seaweed bed |
| CN114097511A (en) * | 2021-11-12 | 2022-03-01 | 海南大学 | Method for rapidly greening tropical and subtropical coastal sandy land |
| WO2023082439A1 (en) * | 2021-11-12 | 2023-05-19 | 海南大学 | Method for quickly greening tropical and subtropical coastal sandy land |
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