CN105557509A - Cold-hardiness breeding method for camellia - Google Patents
Cold-hardiness breeding method for camellia Download PDFInfo
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- CN105557509A CN105557509A CN201510939902.6A CN201510939902A CN105557509A CN 105557509 A CN105557509 A CN 105557509A CN 201510939902 A CN201510939902 A CN 201510939902A CN 105557509 A CN105557509 A CN 105557509A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a cold-hardiness breeding method for camellia. According to the breeding method, a mode of combining radiation breeding and chemical mutation breeding is adopted. The breeding method comprises seed selection, radiation breeding, seed germination culture, screening, chemical mutation breeding, secondary screening and seedling culture. The breeding method is capable of performing high cold resistance culture on camellia and has wide market prospect; the mode of combining radiation breeding and chemical mutation breeding is adopted, so that the mutation effectiveness can be ensured, breakage treatment is not performed on seeds or seedlings, and the treated seedlings are high in survival rate.
Description
Technical field
The invention belongs to field of plant breeding, particularly relate to a kind of cold-tolerance breeding method of camellia.
Background technology
The requirement of modern horticultural to kind is more and more higher, not only requires that flowers have high yield, high-quality, disease-resistant, also will have series of products in pattern, flower pattern, plant type, florescence etc.Camellia is one of large famous flower of China ten, and cultivation history is long, and flower appearance is graceful, and pattern is bright-coloured, and bloom early, the florescence is long, become in a lot of place beautify the environment, decorative family is requisite views and admires treasure.Along with the development of flowers cause, application booth, greenhouse, the measure such as potted plant universal, the region of introducing and planting expands further, all the more extensively.Camellia natural distributed is in south China, and outdoor cropping is mainly distributed in the Yangtze river basin, is many especially with Zhejiang, Fujian, Sichuan, Hunan, Jiangxi, Anhui Zhu Sheng.Microclimate conditions good place in the Huanghe valley also can grow, and Laoshan region following the line of the sea has wild, and there is cultivation on the ground such as Xi'an.How potted plant various places, the north are, and winter enters the room.Therefore, be necessary to develop a kind of can cold-resistant, the camellia breeding method that can adapt to outdoor weather winter, to meet the demand in market.
Chinese patent CN201310594357.2 discloses the propagation method of a kind of camellia management.Sexual propagation method (seminal propagation method), every seed of being obtained by sexual hybridization and single-lobe camellia seed, and half polyphyll camellia even can be solid seed, can in order to breeding.Seed is ripe in October.After fruit harvesting, in time seed should be taken out from capsule, be placed in the wet sand of water content 5% and preserve, in order to nursery when Second Year spring sow by (February).Or in order to carry out winter sowing, but winter sowing than spring sowing emerge Zao, neat, growth is fast.But winter sowing must prevent the plague of rats.If take vernalization to sow when sowing in spring, the effect of winter sowing can be reached equally.After seedling all sprouts, throw off above cover grass, fertilising of can loosening the soil.Start, with fertile, as well thinner.To 7 ~ August, once to cut root, to promote the development growth of side root and fibrous root.Second Year spring, carry out transplanting seedlings potted plant, the further development growth of root system can be made, be conducive to strong sprout.Individual plant camellia flower seedling after transplanting, can carry out routine servicing management by well-established law.But this breeding method cycle is long, cultivate the camellia flower seedling quality instability obtained.
Chinese patent CN201110123557.0 discloses a kind of method of Fast-propagation camellia, comprising: prune giant tea tree branch, and carry out specially treated; Gather required kind scion; Grafting, cuttage, tight tube sealing reason; Specific practice is: (1) gathers branch; (2) scion is gathered; (3) grafting; (4) cuttage; (5) canopy management is entered.But the method can not realize the cold-resistant cultivation of camellia, restricted application.
Therefore a kind of effective camellia cold-tolerance breeding method is badly in need of, to adapt to plant demand widely.
Summary of the invention
The present invention is directed to the problems referred to above, a kind of cold-tolerance breeding method of camellia is provided, the mode that breeding method adopts radiation breeding and chemical mutation to combine, breeding method comprises seed selection, radiation breeding, seed germination cultivation, screening, chemical mutation, postsearch screening and seedling culture; The present invention can carry out the cold performance cultivation of height to camellia, there are market prospects widely, the mode adopting radioinduction and mutagenesis to combine, the validity ensureing mutagenesis can be crossed, do not carry out breakage process to seed or seedling, the seedling percent after process is high.Seed selection is for selecting full grains, camellia seed without mildew; Radiation breeding adopts co-60 radiation mutation breeding, screens and cultivates 7 days ~ 10 days under-7 DEG C ~-10 DEG C conditions into sprouting the seedling after cultivating, choose survival seedling; Being operating as of radiation breeding: with dose rate be 2000 ~ 8000 roentgens/hour 60Co γ-x ray irradiation x camellia seed 2 hours ~ 4 hours, then overturn camellia seed, with dose rate be 2000 ~ 4000 roentgens/hour x-ray irradiation camellia seed 2 hours ~ 4 hours.
Further, seed germination is cultivated and is: seed soaked by radiation breeding in 10 days, vernalization.
Further, seed soaks and is: soaked 2 days ~ 3 days under 25 DEG C ~ 30 DEG C conditions by the seed after radiation breeding.
Further, vernalization is: wrapped by the seed hygenic towelette after soaking, and put into sealed plastic bag, keep 3 days ~ 5 days under 25 DEG C ~ 30 DEG C conditions, open plastic sack every day and ventilate half an hour, then spray a small amount of water, guarantee hygenic towelette is moistening, but without ponding in plastic sack.
Further, the mutagen of chemical mutation comprise: the intermixture of nitrous acid, azanol, base sulfonic acid, sodium azide, 5 bromouracil, 5 bromine deoxyribonucleosides, 5-purine, and the weight portion of described mutagen consists of: nitrous acid 1 part ~ 3 parts, azanol 0.1 part ~ 0.3 part, base sulfonic acid 0.1 part ~ 0.3 part, sodium azide 0.2 part ~ 0.5 part, 5 bromouracil 0.1 part ~ 0.3 part, 5 bromine deoxyribonucleoside 0.1 part ~ 0.3 part, 1 part ~ 5 parts, 5-purine, pure water 1000 parts ~ 2000 parts.
Further, the mutagen compound method of chemical mutation is: be dissolved in the half pure water of according to dosage part by the 5 bromouracil of according to dosage part, 5 bromine deoxyribonucleosides, 5-purine, then nitrous acid, azanol, base sulfonic acid, sodium azide is added successively, add the pure water of second half according to dosage part again, finally regulating the pH of mutagen solution by saturated HCl solution and saturated NaOH solution, is 6.5 ~ 7.0 to pH.
Further, after mutagen configure, put and keep in Dark Place at normal temperatures.
Further, chemical mutation adopts semar technique, being operating as of semar technique: the seedling apical tender shoots sprouted with the cotton balls wiping being moistened with mutagen, every day 2 times ~ 5 times, continuous 5 days ~ 7 days.
Further, postsearch screening is: cultivated 7 days ~ 10 days under-17 DEG C ~-20 DEG C conditions by the seedling after chemical mutation process, and choose survival seedling, selecting further can resistance to high and cold camellia seed.
Further, seedling culture is: by the seedling after postsearch screening temperature 25 DEG C ~ 30 DEG C, sunshine every day 6h ~ 8h, intensity of illumination 80 μm of olm
-2s
-1~ 100 μm of olm
-2s
-1condition under cultivate 15 days, grow under field conditions (factors) after 15 days.The inventive method, while enhancing camellia cold performance, can also improve the resistance to elevated temperatures of camellia further, make the planting range of camellia wider.
Advantage of the present invention is:
1. the present invention can carry out the cold performance cultivation of height to camellia, has market prospects widely;
2. breeding method of the present invention is while enhancing camellia cold performance, can also improve the resistance to elevated temperatures of camellia further, make the planting range of camellia wider;
3. breeding method of the present invention adopts the mode that radioinduction and mutagenesis combine, and can cross the validity ensureing mutagenesis;
4. breeding method of the present invention does not carry out breakage process to seed or seedling, and the seedling percent after process is high.
Except object described above, feature and advantage, the present invention also has other object, feature and advantage.Below with reference to embodiment, the present invention is further detailed explanation.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail, but the multitude of different ways that the present invention can be defined by the claims and cover is implemented.
Embodiment 1
A kind of cold-tolerance breeding method of camellia
Be specially: the mode that breeding method adopts radiation breeding and chemical mutation to combine, breeding method comprises seed selection, radiation breeding, seed germination cultivation, screening, chemical mutation, postsearch screening and seedling culture; Seed selection is for selecting full grains, camellia seed without mildew; Radiation breeding adopts co-60 radiation mutation breeding, screens and cultivates 7 days under-7 DEG C of conditions into sprouting the seedling after cultivating, choose survival seedling; Being operating as of radiation breeding: with dose rate be 2000 roentgens/hour 60Co γ-x ray irradiation x camellia seed 2 hours, then overturn seed, with dose rate be 2000 roentgens/hour x-ray irradiation camellia seed 2 hours.Seed germination is cultivated: seed soaked by radiation breeding in 10 days, vernalization, seed soaks: soaked 2 days under 25 DEG C of conditions by the seed after radiation breeding, vernalization is: wrapped by the seed hygenic towelette after soaking, and put into sealed plastic bag, keep 3 days under 25 DEG C of conditions, open plastic sack every day and ventilate half an hour, then spray a small amount of water, guarantee hygenic towelette is moistening, but without ponding in plastic sack.The weight portion of the mutagen of chemical mutation consists of: nitrous acid 1 part, azanol 0.1 part, base sulfonic acid 0.1 part, sodium azide 0.2 part, 5 bromouracil 0.1 part, 5 bromine deoxyribonucleoside 0.1 part, 1 part, 5-purine, pure water 1000 parts.The mutagen compound method of chemical mutation is: be dissolved in the half pure water of according to dosage part by the 5 bromouracil of according to dosage part, 5 bromine deoxyribonucleosides, 5-purine, then nitrous acid, azanol, base sulfonic acid, sodium azide is added successively, add the pure water of second half according to dosage part again, the pH of mutagen solution is finally regulated by saturated HCl solution and saturated NaOH solution, be 6.5 to pH, after mutagen configure, put and keep in Dark Place at normal temperatures; Chemical mutation adopts semar technique, being operating as of semar technique: the seedling apical tender shoots sprouted with the cotton balls wiping being moistened with mutagen, every day 2 times, continuous 5 days.Postsearch screening is: cultivated 7 days under-17 DEG C of conditions by the seedling after chemical mutation process, chooses survival seedling.Seedling culture is: by the seedling after postsearch screening temperature 25 DEG C, sunshine every day 6h, intensity of illumination 80 μm of olm
-2s
-1condition under cultivate 15 days, grow under field conditions (factors) after 15 days.
Embodiment 2
A kind of cold-tolerance breeding method of camellia
Be specially: the mode that breeding method adopts radiation breeding and chemical mutation to combine, breeding method comprises seed selection, radiation breeding, seed germination cultivation, screening, chemical mutation, postsearch screening and seedling culture; Seed selection is for selecting full grains, camellia seed without mildew; Radiation breeding adopts co-60 radiation mutation breeding, screens and cultivates 10 days under-10 DEG C of conditions into sprouting the seedling after cultivating, choose survival seedling; Being operating as of radiation breeding: with dose rate be 8000 roentgens/hour 60Co γ-x ray irradiation x camellia seed 4 hours, then overturn camellia seed, with dose rate be 4000 roentgens/hour x-ray irradiation camellia seed 4 hours.Seed germination is cultivated: seed soaked by radiation breeding in 10 days, vernalization, seed soaks: soaked 3 days under 30 DEG C of conditions by the seed after radiation breeding, vernalization is: wrapped by the seed hygenic towelette after soaking, and put into sealed plastic bag, keep 5 days under 30 DEG C of conditions, open plastic sack every day and ventilate half an hour, then spray a small amount of water, guarantee hygenic towelette is moistening, but without ponding in plastic sack.The weight portion of chemical mutation consists of: nitrous acid 3 parts, azanol 0.3 part, base sulfonic acid 0.3 part, sodium azide 0.5 part, 5 bromouracil 0.3 part, 5 bromine deoxyribonucleoside 0.3 part, 5 parts, 5-purine, pure water 2000 parts.The mutagen compound method of chemical mutation is: be dissolved in the half pure water of according to dosage part by the 5 bromouracil of according to dosage part, 5 bromine deoxyribonucleosides, 5-purine, then nitrous acid, azanol, base sulfonic acid, sodium azide is added successively, add the pure water of second half according to dosage part again, the pH of mutagen solution is finally regulated by saturated HCl solution and saturated NaOH solution, be 7.0 to pH, after mutagen configure, put and keep in Dark Place at normal temperatures; Chemical mutation adopts semar technique, being operating as of semar technique: the seedling apical tender shoots sprouted with the cotton balls wiping being moistened with mutagen, every day 5 times, continuous 7 days.Postsearch screening is: cultivated 10 days under-20 DEG C of conditions by the seedling after chemical mutation process, chooses survival seedling.Seedling culture is: by the seedling after postsearch screening temperature 30 DEG C, sunshine every day 8h, intensity of illumination 100 μm of olm
-2s
-1condition under cultivate 15 days, grow under field conditions (factors) after 15 days.
Embodiment 3
A kind of cold-tolerance breeding method of camellia
Be specially: the mode that breeding method adopts radiation breeding and chemical mutation to combine, breeding method comprises seed selection, radiation breeding, seed germination cultivation, screening, chemical mutation, postsearch screening and seedling culture; Seed selection is for selecting full grains, camellia seed without mildew; Radiation breeding adopts co-60 radiation mutation breeding, screens and cultivates 8.5 days under-8.5 DEG C of conditions into sprouting the seedling after cultivating, choose survival seedling; Being operating as of radiation breeding: with dose rate be 5000 roentgens/hour 60Co γ-x ray irradiation x camellia seed 3 hours, then overturn seed, with dose rate be 3000 roentgens/hour x-ray irradiation camellia seed 3 hours.Seed germination is cultivated: seed soaked by radiation breeding in 10 days, vernalization, seed soaks: soaked 2.5 days under 27.5 DEG C of conditions by the seed after radiation breeding, vernalization is: wrapped by the seed hygenic towelette after soaking, and put into sealed plastic bag, keep 4 days under 27.5 DEG C of conditions, open plastic sack every day and ventilate half an hour, then spray a small amount of water, guarantee hygenic towelette is moistening, but without ponding in plastic sack.The weight portion of the mutagen of chemical mutation consists of: nitrous acid 2 parts, azanol 0.2 part, base sulfonic acid 0.2 part, sodium azide 0.35 part, 5 bromouracil 0.2 part, 5 bromine deoxyribonucleoside 0.2 part, 3 parts, 5-purine, pure water 1500 parts.The mutagen compound method of chemical mutation is: be dissolved in the half pure water of according to dosage part by the 5 bromouracil of according to dosage part, 5 bromine deoxyribonucleosides, 5-purine, then nitrous acid, azanol, base sulfonic acid, sodium azide is added successively, add the pure water of second half according to dosage part again, the pH of mutagen solution is finally regulated by saturated HCl solution and saturated NaOH solution, be 6.75 to pH, after mutagen configure, put and keep in Dark Place at normal temperatures; Chemical mutation adopts semar technique, being operating as of semar technique: the seedling apical tender shoots sprouted with the cotton balls wiping being moistened with mutagen, every day 3 times, continuous 6 days.Postsearch screening is: cultivated 8.5 days under-18.5 DEG C of conditions by the seedling after chemical mutation process, chooses survival seedling.Seedling culture is: by the seedling after postsearch screening temperature 27.5 DEG C, sunshine every day 7h, intensity of illumination 90 μm of olm
-2s
-1condition under cultivate 15 days, grow under field conditions (factors) after 15 days.
Embodiment 4
A kind of cold-tolerance breeding method of camellia
Be specially: the mode that breeding method adopts radiation breeding and chemical mutation to combine, breeding method comprises seed selection, radiation breeding, seed germination cultivation, screening, chemical mutation, postsearch screening and seedling culture; Seed selection is for selecting full grains, camellia seed without mildew; Radiation breeding adopts co-60 radiation mutation breeding, screens and cultivates 8 days under-8 DEG C of conditions into sprouting the seedling after cultivating, choose survival seedling; Being operating as of radiation breeding: with dose rate be 2800 roentgens/hour 60Co γ-x ray irradiation x camellia seed 3 hours, then overturn seed, with dose rate be 2400 roentgens/hour x-ray irradiation camellia seed 3 hours.Seed germination is cultivated: seed soaked by radiation breeding in 10 days, vernalization, seed soaks: soaked 2 days under 27 DEG C of conditions by the seed after radiation breeding, vernalization is: wrapped by the seed hygenic towelette after soaking, and put into sealed plastic bag, keep 4 days under 28 DEG C of conditions, open plastic sack every day and ventilate half an hour, then spray a small amount of water, guarantee hygenic towelette is moistening, but without ponding in plastic sack.The weight portion of the mutagen of chemical mutation consists of: nitrous acid 1 part, azanol 0.2 part, base sulfonic acid 0.3 part, sodium azide 0.4 part, 5 bromouracil 0.3 part, 5 bromine deoxyribonucleoside 0.2 part, 1 part, 5-purine, pure water 1200 parts.The mutagen compound method of chemical mutation is: be dissolved in the half pure water of according to dosage part by the 5 bromouracil of according to dosage part, 5 bromine deoxyribonucleosides, 5-purine, then nitrous acid, azanol, base sulfonic acid, sodium azide is added successively, add the pure water of second half according to dosage part again, the pH of mutagen solution is finally regulated by saturated HCl solution and saturated NaOH solution, be 6.8 to pH, after mutagen configure, put and keep in Dark Place at normal temperatures; Chemical mutation adopts semar technique, being operating as of semar technique: the seedling apical tender shoots sprouted with the cotton balls wiping being moistened with mutagen, every day 3 times, continuous 6 days.Postsearch screening is: cultivated 9 days under-19 DEG C of conditions by the seedling after chemical mutation process, chooses survival seedling.Seedling culture is: by the seedling after postsearch screening temperature 28 DEG C, sunshine every day 7h, intensity of illumination 89 μm of olm
-2s
-1condition under cultivate 15 days, grow under field conditions (factors) after 15 days.
Experimental example
In this experimental example, test the breeding effect of the present invention 1 ~ 4 method by experiment.
1. test material
Same camellia seed 2kg, 1kg is as experimental group, and 1kg as a control group.
Experimental group: according to the camellia growth of seedling situation of seedling after the breeding of the embodiment of the present invention 1 ~ 4 method;
Control group: the camellia growth of seedling situation obtained with common breeding method process and output.
2. test method
Camellia growing state after the test embodiment of the present invention 1 ~ 4 breeding, the situation statistics of camellia growth of seedling situation obtained with common breeding method process.
3. result of the test
Analyze after the situation statistics of the camellia growing state after the embodiment of the present invention 1 ~ 4 breeding, the camellia growing state obtained with common breeding method process and output, experimental result is in table 1.
Table 1. test result
| Material | Minimum tolerable temperature (DEG C) | Florescence | The highest tolerable temperature (DEG C) |
| The seedling that embodiment 1 breeding method is cultivated | -27 | The 4-6 month | 40 |
| The seedling that embodiment 2 breeding method is cultivated | -28 | The 4-6 month | 42 |
| The seedling that embodiment 3 breeding method is cultivated | -26 | The 4-6 month | 43 |
| The seedling that embodiment 4 breeding method is cultivated | -26 | The 4-6 month | 41 |
| The seedling that common breeding method is cultivated | -9 | The 2-3 month | 35 |
Experimental result shows, compared with the camellia seedling cultivated with common breeding method, the highest tolerable temperature of the seedling that low, the highest tolerable temperature of minimum tolerable temperature of the embodiment of the present invention 1 ~ 4 breeding method seedling that the minimum tolerable temperature of camellia is cultivated than common breeding method under square one is cultivated than common breeding method is high, florescence is longer, and the planting range be suitable for is wider.
These are only the preferred embodiments of the present invention and experimental example, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., within the protection domain that all should be included in invention.
Claims (10)
1. the cold-tolerance breeding method of a camellia, the mode that described breeding method adopts radiation breeding and chemical mutation to combine, it is characterized in that, described breeding method comprises seed selection, radiation breeding, seed germination cultivation, screening, chemical mutation, postsearch screening and seedling culture; Described seed selection is select full grains, camellia seed without mildew; Described radiation breeding adopts co-60 radiation mutation breeding, and described screening is sprout the seedling after cultivating to cultivate 7 days ~ 10 days under-7 DEG C ~-10 DEG C conditions, chooses survival seedling; Being operating as of described radiation breeding: with dose rate be 2000 ~ 8000 roentgens/hour
60co γ-x ray irradiation x camellia seed 2 hours ~ 4 hours, then overturns camellia seed, with dose rate be 2000 ~ 4000 roentgens/hour x-ray irradiation camellia seed 2 hours ~ 4 hours.
2. breeding method according to claim 1, is characterized in that, described seed germination is cultivated and is: seed soaked by radiation breeding in 10 days, vernalization.
3. breeding method according to claim 2, is characterized in that, described seed soaks and is: soaked 2 days ~ 3 days under 25 DEG C ~ 30 DEG C conditions by the seed after radiation breeding.
4. breeding method according to claim 2, it is characterized in that, described vernalization is: wrapped by the seed hygenic towelette after soaking, and put into sealed plastic bag, keep 3 days ~ 5 days under 25 DEG C ~ 30 DEG C conditions, open plastic sack every day and ventilate half an hour, then spray a small amount of water, guarantee hygenic towelette is moistening, but without ponding in plastic sack.
5. breeding method according to claim 1, it is characterized in that, the mutagen of described chemical mutation comprise: the intermixture of nitrous acid, azanol, base sulfonic acid, sodium azide, 5 bromouracil, 5 bromine deoxyribonucleosides, 5-purine, and the weight portion of described mutagen consists of: nitrous acid 1 part ~ 3 parts, azanol 0.1 part ~ 0.3 part, base sulfonic acid 0.1 part ~ 0.3 part, sodium azide 0.2 part ~ 0.5 part, 5 bromouracil 0.1 part ~ 0.3 part, 5 bromine deoxyribonucleoside 0.1 part ~ 0.3 part, 1 part ~ 5 parts, 5-purine, pure water 1000 parts ~ 2000 parts.
6. breeding method according to claim 5, it is characterized in that, the mutagen compound method of described chemical mutation is: be dissolved in the half pure water of according to dosage part by the 5 bromouracil of according to dosage part, 5 bromine deoxyribonucleosides, 5-purine, then nitrous acid, azanol, base sulfonic acid, sodium azide is added successively, add the pure water of second half according to dosage part again, finally regulating the pH of mutagen solution by saturated HCl solution and saturated NaOH solution, is 6.5 ~ 7.0 to pH.
7. breeding method according to claim 6, is characterized in that, after described mutagen configure, puts and keeps in Dark Place at normal temperatures.
8. breeding method according to claim 1, is characterized in that, described chemical mutation adopts semar technique, being operating as of described semar technique: the seedling apical tender shoots sprouted with the cotton balls wiping being moistened with mutagen, every day 2 times ~ 5 times, continuous 5 days ~ 7 days.
9. breeding method according to claim 1, is characterized in that, described postsearch screening is: cultivated 7 days ~ 10 days under-17 DEG C ~-20 DEG C conditions by the seedling after chemical mutation process, chooses survival seedling.
10. breeding method according to claim 1, is characterized in that, described seedling culture is: by the seedling after postsearch screening temperature 25 DEG C ~ 30 DEG C, sunshine every day 6h ~ 8h, intensity of illumination 80 μm of olm
-2s
-1~ 100 μm of olm
-2s
-1condition under cultivate 15 days, grow under field conditions (factors) after 15 days.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106856706A (en) * | 2016-12-26 | 2017-06-20 | 姚春光 | A kind of processing method of three-coloured amaranth seed |
| CN107396800A (en) * | 2017-08-24 | 2017-11-28 | 广西沙田仙人滩农业投资有限公司 | A kind of cultural method of camellia |
-
2015
- 2015-12-16 CN CN201510939902.6A patent/CN105557509A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106856706A (en) * | 2016-12-26 | 2017-06-20 | 姚春光 | A kind of processing method of three-coloured amaranth seed |
| CN107396800A (en) * | 2017-08-24 | 2017-11-28 | 广西沙田仙人滩农业投资有限公司 | A kind of cultural method of camellia |
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Application publication date: 20160511 |
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