CN115486318A - Method for sexual propagation of Hainan pseudo-Shaosao - Google Patents
Method for sexual propagation of Hainan pseudo-Shaosao Download PDFInfo
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- CN115486318A CN115486318A CN202211459408.6A CN202211459408A CN115486318A CN 115486318 A CN115486318 A CN 115486318A CN 202211459408 A CN202211459408 A CN 202211459408A CN 115486318 A CN115486318 A CN 115486318A
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- pseudo
- seeds
- yannan
- shaosao
- shaoshanzi
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Links
- 238000000034 method Methods 0.000 title claims abstract description 37
- 230000001568 sexual effect Effects 0.000 title claims description 7
- 230000035784 germination Effects 0.000 claims abstract description 21
- 239000011159 matrix material Substances 0.000 claims abstract description 16
- 230000014639 sexual reproduction Effects 0.000 claims abstract description 7
- 238000012360 testing method Methods 0.000 claims abstract description 5
- 239000002689 soil Substances 0.000 claims abstract description 3
- 238000009331 sowing Methods 0.000 claims description 13
- 235000013399 edible fruits Nutrition 0.000 claims description 10
- 239000003630 growth substance Substances 0.000 claims description 9
- 238000010899 nucleation Methods 0.000 claims description 8
- 230000004083 survival effect Effects 0.000 claims description 8
- 239000012286 potassium permanganate Substances 0.000 claims description 7
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 6
- 244000060011 Cocos nucifera Species 0.000 claims description 6
- 239000000758 substrate Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000009395 breeding Methods 0.000 claims description 4
- 238000003973 irrigation Methods 0.000 claims description 4
- 230000002262 irrigation Effects 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- 238000005553 drilling Methods 0.000 claims description 3
- 238000002834 transmittance Methods 0.000 claims description 3
- 230000001488 breeding effect Effects 0.000 claims description 2
- 230000000249 desinfective effect Effects 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 239000010451 perlite Substances 0.000 claims description 2
- 235000019362 perlite Nutrition 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000011161 development Methods 0.000 abstract description 4
- 230000018109 developmental process Effects 0.000 abstract description 4
- 241000894007 species Species 0.000 description 12
- 229930191978 Gibberellin Natural products 0.000 description 6
- 239000003448 gibberellin Substances 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 5
- 239000000122 growth hormone Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 3
- 102000018997 Growth Hormone Human genes 0.000 description 2
- 108010051696 Growth Hormone Proteins 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011066 ex-situ storage Methods 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 239000005980 Gibberellic acid Substances 0.000 description 1
- 235000019738 Limestone Nutrition 0.000 description 1
- 244000183331 Nephelium lappaceum Species 0.000 description 1
- 235000015738 Nephelium lappaceum Nutrition 0.000 description 1
- 241000557736 Paranephelium Species 0.000 description 1
- 241001093760 Sapindaceae Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 239000006028 limestone Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Abstract
The invention discloses a method for sexual reproduction of Hainan pseudo-Shaoshanzi, which comprises a series of management steps of selecting test site conditions, seedbed matrix, seed collection method, seed treatment, soil matrix and the like. The invention solves the problems of small quantity of seeds, low germination rate, poor seedling stability and the like of the Yangtze Shaosao seeds, and has important scientific and practical significance for the protection and development of the extremely dangerous species.
Description
Technical Field
The invention relates to the technical field of seed seedling culture, in particular to a method for sexual propagation of Yannan pseudo-Shaoshanzi, which comprises the steps of collecting the seeds of the Yannan pseudo-Shaoshanzi and culturing seedlings.
Background
pseudo-Shaosao in Hainan province(Paranephelium hainanensis) Belongs to the sapindaceae pseudo-Shaoshanzi genus evergreen arbor tree species, and Chinese special species are only distributed in the county of south China sea cliff. Because the number of the plants is extremely rare and the distribution area is narrow, the plants are listed as the extremely dangerous species (CR) by IUCN at present and are also listed as II-grade protective plants and extremely small species of population in China. Early studies showed that the ecological system of the area has suffered to some extent from the sea-south false-nephelium lappaceum in tropical lowland secondary broadleaf forests, limestone mountains, whose habitat is extremely fragile, coupled with jamming activity. The research also shows that the wild population of the Yangtze Shaosao is in decline, the natural renewal is rare, and the renewal is difficult. Secondly, the number of seeds of the Yannan pseudo-Shaosao is small, the germination rate of the seeds is low, and large-scale seedling culture is difficult to achieve. The previous project group research shows that the average root length, the number of the adventitious roots and the longest root length after cuttage are the largest after the cuttage is carried out in the double Gelle-GGR with the growth regulator concentration of 400mg/L for 4 hours, the root system effect index is the highest, but the large-scale seedling raising cannot be met. The method is specifically characterized in that (1) in the aspect of ex-situ protection, the growth potential of the cutting seedlings is weaker than that of the seedling seedlings; (2) In the aspect of in-situ protection, the adaptability of the cutting seedlings to the habitat is weaker than that of the seedling. Therefore, there is a need for sexual propagation of the hainan pseudo-shaosao, which includes collecting the seeds of the hainan pseudo-shaosao, treating the seeds to grow seedlings, providing high-quality seedling number for field regression and ex-situ protection of the hainan pseudo-shaosao, and having important scientific and practical significance for protection and development of the extremely dangerous species.
Disclosure of Invention
The invention aims to: the method for sexual propagation of the Yannan pseudo-Shaoshanzi comprises a method from collection of Yannan pseudo-Shaoshanzi seeds to seedling raising of the Yannan pseudo-Shaoshanzi, improves the germination rate of the seeds, provides high-quality seedling quantity for the transition protection and field regression of the Yannan pseudo-Shaoshanzi, is beneficial to the protection and development of the extremely-dangerous species, and has important scientific and practical significance.
The invention is realized by adopting the following technical scheme: a method for sexual propagation of Yannan pseudo-Shaosao includes breeding and seedling raising, and is characterized in that the germination rate of the bred seeds is more than 40%, the survival rate of the seedling raising is more than 90%, and the breeding method includes the following steps:
(1) Collecting seeds of the Yangnan pseudo-Shaosao;
(2) Sowing seeds of the Yannan pseudo-Shaosao;
(3) Germination of the seeds of the Yangnan pseudo-Shaosao;
the method comprises the following specific steps:
(1) The method for collecting the seeds of the Yannan pseudo-Shaosao comprises the following steps:
(1.1) collecting naturally growing nearly mature fruits from the Yangtze Yangshao trees;
(1.2) airing the collected fruits until shells of the fruits naturally burst open, taking out the seeds of the Yannan pseudo-Shaoshanzi, disinfecting the seeds by using potassium permanganate with the concentration of 0.5%, and airing;
(1.3) soaking the dried Yannan pseudo-Shaosao seeds in a growth regulator double-Jier-GGR with the concentration of 200 mg/L, and treating to obtain the Yannan pseudo-Shaosao seeds for sowing;
(2) The method for sowing the seeds of the Yangnan pseudo-Shaosao seeds comprises the following steps:
(2.1) conditions of the test: a nursery garden where seeds of the Yangtze Shaoshanzi are sowed needs a shadow shed with light transmittance of 40 to 50 percent, and the temperature of the greenhouse is adjusted to be 25 to 35 ℃ and the humidity is adjusted to be 40 to 60 percent through a sprinkler irrigation system;
(2.2) seeding bed matrix: sterilizing the matrix of the seeding bed by adopting potassium permanganate with the concentration of 3-5 per mill, wherein the matrix of the seeding bed comprises coconut coir and sandy soil with the water content of 40-60%;
(2.3) sowing the Yannan pseudo-Shaoshanzi seeds for sowing obtained by the method for collecting the Yannan pseudo-Shaoshanzi seeds in a disinfected sowing seedbed matrix in a drilling manner;
(3) The method for germination of the seeds of the Yannan pseudo-Shaosao comprises the following steps:
and (3) sprinkling the sowed seedbed matrix by a sprinkling irrigation system regularly, keeping the humidity between 40 and 60 percent, and waiting for sprouting and seedling growing.
Preferably, the method for transplanting the germinated and grown seedlings of the seeds of the Yangtze Shaoshanzi to a seedling bag for seedling culture comprises the following steps:
(1) Making a seedling raising bag;
(2) Seedling raising;
the method specifically comprises the following steps:
(1) The method for manufacturing the seedling raising bag comprises the following steps:
(1.1) mixing the coconut husk, the perlite and the sandy loam according to the volume ratio of 1:1:2, mixing to prepare a seedling culture substrate;
(1.2) adopting a potassium permanganate solution with the concentration of 3 to 5 per mill to disinfect the seedling culture substrate;
(1.3) subpackaging the sterilized seedling raising matrix into small bags;
(2) The seedling raising method comprises the following steps:
(2.1) when the seedling of the Yannan pseudo-Shaoshanzi is higher than 5cm, growing 2 to 4 true leaves, and transferring the leaves into a seedling bag to finish the seedling of the Yannan pseudo-Shaoshanzi.
Preferably, in the step (2.2), the volume of coconut chaff and sandy loam in the matrix of the seeding bed is 2:1, mixing and preparing.
Preferably, the ripeness of the fruits collected in the step (1.2) is between 70 and 80 percent, and the fruits are naturally dried in the air for 3 to 5 days in a laboratory.
Preferably, the growth regulator bigill-GGR with the concentration of 200 mg/L in the step (1.3) is dissolved by warm water at 50-60 ℃, the soaking time is optimal for 6 h, and the germination rate of seeds is highest.
Preferably, the seeds of the Yangtze Yangshao in the step (2.3) are sowed in the depth of 0.5-1.0 cm of the matrix of the sowing seedbed.
In the invention, the method for collecting the Hainan pseudo-Shaosao seeds is explained and the influence of different hormone concentrations on the germination rate of the Hainan pseudo-Shaosao seeds is compared. Compared with a control group, the growth regulator can improve the germination rate of the seeds and the survival rate of the seedlings of the Yannan pseudo-Shaoshanzi, but the double-Jier-GGR with the concentration of 200 mg/L is used for soaking for 6 h optimally, so that the germination rate of the seeds is highest.
In the invention, the collection and seedling raising method is simple to operate, has obvious effect and has important application value.
Detailed Description
The invention is further illustrated by the following specific examples, the seedling raising method comprises
The method comprises the following steps:
(1) The experiments were carried out in the greenhouse of the institute of forestry science, third city, hainan province. The seeds of the Yangnan false Shaosao are disinfected by potassium permanganate with the concentration of 0.5 percent.
(2) And (3) experimental design: the seeds of the Yannan pseudo-Shaoshanzi are treated by 2 hormones of gibberellin and bigill-GGR with the concentrations of 50 mg/L, 100mg/L, 200 mg/L and 400mg/L respectively, and are soaked for 6 hours by treating the seeds with deionized water as a control group. Each treatment group was set to 3 replicates, each replicate 10.
(3) As a result: as shown in table 1 below:
TABLE 1 comparison of growth regulators at different concentrations on the germination rate of Yannan pseudo-Shaosao seeds
| Growth hormone types | 0 mg/L | 50 mg/L | 100 mg/L | 200 mg/L | 400 mg/L |
| Gibberellins | 3.33 | 10.00 | 20.00 | 30.00 | 23.33 |
| Double Gill-GGR | 3.33 | 13.33 | 23.33 | 43.33 | 10.00 |
The test result shows that the germination rate of the seeds in each treatment group is higher than that of the control group (0 mg/L) after the seeds are sown for 15 hours, and the germination rate of the double-Gel-GGR treatment group with the concentration of 200 mg/L is highest and is 43.3 percent. With the increase of the concentrations of gibberellin and double-Jier-GGR, the germination rates of the seeds of the Yannan pseudo-Shaoshanzi are in a trend of increasing first and then decreasing.
(4) And (3) after the seedlings grow to 2 to 4 true leaves with the height of more than 5cm, transferring the seedlings into a seedling growing bag, and curing the seedlings by a conventional method while keeping certain humidity and light transmittance.
(5) As a result: as shown in table 2 below:
TABLE 2 comparison of growth regulators of different concentrations on the survival rate of the young seedlings of Yannan pseudo-Shaosao seeds
| Growth hormone type | 0 mg/L | 50 mg/L | 100 mg/L | 200 mg/L | 400 mg/L |
| Gibberellins | 0 | 33.33 | 66.67 | 88.89 | 57.14 |
| Double Gill-GGR | 0 | 50.00 | 71.43 | 92.31 | 66.67 |
Test results show that after the seedlings obtained after the Yangtze Shaoshanzi seeds germinate grow for 30 days, the growth hormones with different concentrations also promote the survival rate of the seedlings, and the survival rate of the double-gill-GGR treatment group with the concentration of 200 mg/L is the highest and is 92.31%. With the increase of the concentrations of the two growth hormones, the survival rate of the seedlings also shows a trend of increasing first and then decreasing. Therefore, the concentration of 200 mg/L double Jier-GGR is the highest for the germination rate of the seeds and the survival rate of the seedlings of the Yannan pseudo-Shaoshan.
The gibberellin and the double-Gill-GGR growth regulator have different composition contents and are soluble powder. The content of the gibberellin effective component is more than or equal to 20 percent, and the content of the gibberellic acid is more than or equal to 3 percent; the double Gill-GGR contains more than or equal to 20 percent of amino acid, more than or equal to 2 percent of trace element, less than or equal to 5 mg/kg of limiting element Hg, less than or equal to 10 mg/kg of As, less than or equal to 10 mg/kg of Cd, less than or equal to 50 mg/kg of Pb and less than or equal to 50 mg/kg of Cr.
The breakthrough of the propagation technology is the core task of solving the extremely-dangerous research of rare and extremely-dangerous species and is the important basis for expanding the species population development of the species. Scientifically collecting the seeds of the Yangnan false nephelium lappaceum and performing sexual reproduction, improving the germination rate and the preservation rate of seedlings, and having important significance for breakthrough of the propagation technology of the extremely-dangerous species. In general, the Yangtze Shaosao is used as a critical species and a national II-grade protective plant, and in order to save, protect and recover the critical plant resource, the research of seed collection, seed germination, seedling growth and seedling raising methods is necessary to provide theoretical and technical support for protecting and recovering the resource of the species.
Claims (6)
1. A method for sexual propagation of Yannan pseudo-Shaosao includes breeding and seedling raising, and is characterized in that the germination rate of the bred seeds is more than 40%, the survival rate of the seedling raising is more than 90%, and the breeding method includes the following steps:
collecting seeds of the Yannan pseudo-Shaosao;
sowing seeds of the Yannan pseudo-Shaosao;
germination of seeds of the Yannan pseudo-Shaosao;
the method specifically comprises the following steps:
the method for collecting the seeds of the Yannan pseudo-Shaosao comprises the following steps:
(1.1) collecting naturally growing nearly mature fruits from the Yangtze Yangshao trees;
(1.2) airing the collected fruits until fruit shells are naturally cracked open, taking out the seeds of the Yannan pseudo-Shaoshanzi, and then disinfecting and airing the seeds by using potassium permanganate with the concentration of 0.5%;
(1.3) soaking the dried Yannan pseudo-Shaosao seeds in a growth regulator double-Jier-GGR with the concentration of 200 mg/L, and treating to obtain the Yannan pseudo-Shaosao seeds for sowing;
(2) The method for sowing the seeds of the Yangnan pseudo-Shaosao seeds comprises the following steps:
(2.1) conditions of the test: a nursery garden where seeds of the Yangtze Shaoshanzi are sowed needs a shadow shed with light transmittance of 40 to 50 percent, and the temperature of the greenhouse is adjusted to be 25 to 35 ℃ and the humidity is adjusted to be 40 to 60 percent through a sprinkler irrigation system;
(2.2) seeding bed matrix: sterilizing the matrix of the seeding bed by adopting potassium permanganate with the concentration of 3-5 per mill, wherein the matrix of the seeding bed comprises coconut coir and sandy soil with the water content of 40-60%;
(2.3) sowing the Yannan pseudo-Shaoshanzi seeds for sowing obtained by the method for collecting the Yannan pseudo-Shaoshanzi seeds in a disinfected sowing seedbed matrix in a drilling manner;
(3) The method for germination of the seeds of the Yannan pseudo-Shaosao comprises the following steps:
and (3) sprinkling the sowed seedbed matrix by a sprinkling irrigation system regularly, keeping the humidity within 40-60%, and waiting for sprouting and seedling growing.
2. The method for sexual reproduction of Yannan pseudo-Shaosao as claimed in claim 1, wherein the Yannan pseudo-Shaosao seeds are transplanted to a nursery bag for growing seedlings after germination and growing seedlings, comprising the following steps:
making a seedling raising bag;
seedling raising;
the method specifically comprises the following steps:
(1) The method for manufacturing the seedling raising bag comprises the following steps:
(1.1) mixing the coconut chaff, the perlite and the sandy loam according to the volume ratio of 1:1:2, mixing to prepare a seedling substrate;
(1.2) adopting potassium permanganate solution with the concentration of 3 to 5 per thousand to disinfect the seedling culture substrate;
(1.3) subpackaging the sterilized seedling raising matrix into small bags;
(2) The seedling raising method comprises the following steps:
(2.1) when the seedling of the Yannan pseudo-Shaoshanzi is higher than 5cm, growing 2 to 4 true leaves, and transferring the leaves into a seedling bag to finish the seedling of the Yannan pseudo-Shaoshanzi.
3. The method of sexual reproduction of haynangyao haoshanzi as claimed in claim 1, wherein in step (2.2) the volume of coconut coir and sandy loam in the seeding bed substrate is 2:1, mixing and preparing.
4. The method for sexual reproduction of the Hainan pseudo-Shaoshan as claimed in claim 1, wherein the maturity of the collected fruits in the step (1.2) is between 70 to 80%, and the collected fruits are naturally dried in the air in a laboratory for 3 to 5 days.
5. The method for sexual reproduction of haynangnan pseudo-nephelium lappaceum as claimed in claim 1, wherein said growth regulator bigill-GGR concentration of 200 mg/L in step (1.3) is dissolved in warm water at 50-60 ℃ for 6 h, and the germination rate of seeds is more than 40%.
6. The method for sexual reproduction of Hainan pseudo-Shaoshanzi as claimed in claim 1, wherein said step (2.3) of drilling said seeds of Hainan pseudo-Shaoshanzi at a depth of 0.5-1.0 cm in said bed of sown seedlings.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN117204165A (en) * | 2023-11-08 | 2023-12-12 | 三亚市林业科学研究院 | Sexual propagation method of Hainan pseudoshao seeds |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117204165A (en) * | 2023-11-08 | 2023-12-12 | 三亚市林业科学研究院 | Sexual propagation method of Hainan pseudoshao seeds |
| CN117204165B (en) * | 2023-11-08 | 2024-02-02 | 三亚市林业科学研究院 | Sexual propagation method of Hainan pseudoshao seeds |
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Application publication date: 20221220 |