CN102119621A - Artificial seedling cultivation method of endangered medicinal plant Himalayan mayapple - Google Patents
Artificial seedling cultivation method of endangered medicinal plant Himalayan mayapple Download PDFInfo
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- CN102119621A CN102119621A CN201010578491XA CN201010578491A CN102119621A CN 102119621 A CN102119621 A CN 102119621A CN 201010578491X A CN201010578491X A CN 201010578491XA CN 201010578491 A CN201010578491 A CN 201010578491A CN 102119621 A CN102119621 A CN 102119621A
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Abstract
The invention discloses an artificial seedling cultivation method of an endangered medicinal plant Himalayan mayapple to solve the problems of a low seed germination rate, poor emergence and a long seedling cultivation period at a seedling cultivation stage in the conventional planting process of the Himalayan mayapple. The artificial seedling cultivation method comprises comprehensive methods such as potassium permanganate seed disinfection, growth promoter seed soaking and pregermination, promotion of seedling euphylla generation and growth, and the like; and compared with the conventional seedling cultivation technology, the artificial seedling cultivation method has the advantages that: the seed germination rate of the Himalayan mayapple is doubled, the seedling cultivation rate is doubled, the seedling cultivation time is shortened to 8 months, and the seedling transplantation time is advanced by one growth period so as to lay the foundation for shortening the forming time of a Himalayan mayapple commercial medicinal material.
Description
Technical field
The invention belongs to wild plant artificial cultivation, acclimatization technology field, relate to and use plant growth regulator to carry out polytechnic reasonable utilizations such as presprouting of seeds and promotion growth of seedling.
Background technology
Chinese podophyllum root is that the Berberidaceae Chinese podophyllum root belongs to herbaceos perennial, contain a large amount of lignanoids materials in its root and the rhizome with active anticancer, wherein the podophyllotoxin active anticancer is the highest, be synthetic GP7, therefore the initial substance of cancer therapy drug such as VP-16 and VM-26 becomes one of important anti-cancer medicament raw material medicine.Since the nineties in last century, along with the increase of world market demand, wild Chinese podophyllum root is suffered to excavate on a large scale, in addition himself fertility a little less than, wild resource is endangered, population quantity sharply descends.Chinese podophyllum root was included by " Chinese Plants Red Data Book " in 1992.Therefore adopting artificial domesticating cultivation is one of effective way that solves the in imminent danger and market supply of its resource.
Chinese podophyllum root mainly is grown in the alpine meadow, cheuch sylvan life, shrubbery of height above sea level 2700-4500m, in the rock crevice or spacious meadow, based on self-pollination, short (the early and middle ten days sprouting in May of year growth cycle, mid or late October is withered), the fate of effectively growing in year is 90-120 days, height above sea level, amblent air temperature, soil condition is required very harsh.Chinese podophyllum root is generally based on seminal propagation, and individual month resting stage could sprout but seed demand passes through 6-9, grows up to the commodity medicinal material and needs the 5-6 year usually.
The tame record of Chinese podophyllum root starts from 19th-century, and the traditional artificial cultivation method that continues into the present is: the Chinese podophyllum root seed that mid or late August gathers fresh, and do the seedbed drilling of high furrow or broadcast sowing.Seed began to germinate in mid or late April next year, mid or late September grows up to 1-2 sheet true leaf (most 1) seedling, transplant immediately or transplant the 3rd year spring, i.e. at least one year of seedling raise period continuity is to a year and a half, and has low, irregular, the problem such as the transplanted seedling true leaf is few of emerging of percentage of seedgermination.Therefore, improve percentage of seedgermination and shorten seedling raise period, cultivate and just become one of key link in the Chinese podophyllum root artificial cultivation technique strong sprout.
Summary of the invention
The purpose of this invention is to provide a kind of medicinal plant Chinese podophyllum root artificial raise seedling method in imminent danger, to solve the problem that low, the neat seedling of stage percentage of seedgermination is poor, growing-seedling period is long of growing seedlings in the Chinese podophyllum root tradition planting process.
The present invention includes following steps:
A, presprouting of seeds: use wild Chinese podophyllum root seed, after sorting, dry surface moisture; Can adopt weight percent concentration to wild Chinese podophyllum root seed separation is the saline solution of 25%-30%.The seed weight percent concentration of drying surface moisture after sorting is that 0.5% liquor potassic permanganate at room temperature soaks 2h, uses Gibberellins solution that concentration is 200mg/L, 4 ℃ again instead and soaks 46h; Remove the moisture of the surface of the seed, can sow;
Wild Chinese podophyllum root seed is through at least 2 months wild Chinese podophyllum root seed of 4 ℃ of low temperature storages after the fresh wild seed gathered then or the fresh then collection.
B, cave dish sowing: humus soil is packed in the seedling culture hole plate, with concentration is that 50% carbendazol wettable powder is diluted with water to 800-1000 solution doubly, soil is 2 times in the dish of spray disinfectant cave, 2 seeds after the vernalization are sowed in every cave at random, degree of depth 2cm gets final product, earthing coils the cave short its sprouting in the environment that is positioned over temperature 17-22 ℃;
C, promote true leaf to take place and growth: waiting to germinate finish after, the continuous spraying concentration of cotyledon is the gibberellin 7 times of 200mg/L, totally 7 days once a day, root was executed the kinetin 3 times that concentration is 40mg/L then, executes 1 time in per 5 days; The growth of seedling environmental temperature is 17-22 ℃;
D, transplanting: above seedling is kept away the high light direct projection in early and middle ten days transplanting land for growing field crops in May.
The present invention is directed to the biological property of medicinal plant Chinese podophyllum root in imminent danger, the method that adopts physics (temperature) and biological control measure (plant growth regulator) to combine, key has been considered concentration and the processing time and the treatment temperature of gibberellin, pass through application concentration, time, the scientific arrangement in temperature and period, integrated application presprouting of seeds and growth technology, compare traditional seedling-cultivating method, patent success of the present invention the Chinese podophyllum root percentage of seedgermination is brought up to 70% from 35%, the beginning germinating time advanceed to 40 days from 7 months, emergence rate brings up to 62% from 31%, seedling raise period foreshortens to 4 months, and the time of seedling replanting is carried previous growth cycle.Behind the seedling replanting, in vegetative period then, the true leaf number has only the 1-2 sheet to be increased to the 2-4 sheet from traditional seedling growing process, helps the plant growth of later stage.
Because the underground root-like stock prosperity of Chinese podophyllum root, so the present invention promotes the differentiation of its underground bud original hase, thereby helps the formation of plant true leaf by Chinese podophyllum root seedling root being executed kinetin (N6-furans methylamino purine), cultivates strong sprout.
Technical solution of the present invention has the following advantages:
1) shortened germinating time, improved germination rate: after planting begin about 40 days to germinate, left and right sides germination rate reached 70% in 80 days;
2) seedling raise period shortens, and the emergence rate height: seedling raise period foreshortens to 4 months, and emergence rate is brought up to about 62%;
3) transplanted seedling stalwartness: behind the seedling replanting, in vegetative period then, the true leaf number reaches the 2-4 sheet, helps the raising of the biomass of plant;
4) use Chinese podophyllum root artificial raise seedling method of the present invention; not only improve seed germination rate, shorten seedling raise period 8 months; and the transplanted seedling true leaf number that obtains is many; helping shortening the commodity medicinal material becomes a useful person the time; increase output; for the medicinal herb grower increases income, the present situation that also can realize protecting wild Chinese podophyllum root habitat ecotope and solve its scarcity of resources, social benefit and economic benefit are obvious.
Embodiment
Embodiment 1:
1) seed treatment: get fresh wild Chinese podophyllum root seed, through tintometry (25% saline solution) sorting, running water is cleaned, and is 0.5% potassium permanganate soaking at room temperature 2h with weight percent concentration, use the Gibberellins solution that concentration is 200mg/L, 4 ℃ of immersion 46h again instead, dry moisture;
2) cave dish sowing: get the plastics cave dish in 72 holes, the filling humus soil is 2 sterilizations of carbendazol wettable powder dilute with water 800-1000 spray solution soil doubly of 50% with concentration, treats soil moisture content suitable back sowing.2 seeds in every cave, the about 2cm of the degree of depth, earthing (through sterilization).The control temperature is urged its germination between 17-22 ℃.Begin before and after after planting 40 days to germinate, germination rate reaches about 70% before and after 80 days.
3) promote true leaf to take place and growth: will go up the Chinese podophyllum root seedling that sprouts in the dish of step cave, cotyledon sprays the gibberellin 7 times (once a day) of 200mg/L continuously, root is executed the kinetin 3 times (per 5 days once) of 40mg/L then, and the growth of seedling environmental temperature is between 17-22 ℃; From counting the day of sowing, begin to grow rough leaf before and after 98 days, go out the true leaf seedling before and after 120 days and reach about 90%;
4) transplant: above-mentioned seedling is taken the shade net lucifuge after May, early and middle ten days was transplanted the land for growing field crops then, and 3 first quarter moons of growing are to early September, plant true leaf number 2-4 sheet.
Embodiment 2
Gather then, 2 months wild Chinese podophyllum root seed of 4 ℃ of low temperature storages, after tintometry (30% saline solution) sorting, running water is cleaned, with weight percent concentration 0.5% potassium permanganate soaking at room temperature 2h, use the gibberellin of 200mg/L, 4 ℃ of immersion 46h instead, dry moisture, cave dish sowing (method is with embodiment 1), the control room temperature is between 17-22 ℃.40 days left and right sides seeds begin to germinate, and left and right sides germination rate reached about 70% in 80 days; The seedling cotyledon that germinates is sprayed the gibberellin 7 times (once a day) of 200mg/L continuously, and root is executed the kinetin 3 times (per 5 days once) of 40mg/L then.From counting the day of sowing, begin to grow rough leaf about 98 days, go out the true leaf seedling about 120 days and reach 62%; May, the shade net lucifuge was taken in early and middle ten days seedling replanting land for growing field crops, and 3 first quarter moons of growing are to early and middle ten days in September, field seedling true leaf number 2-4 sheet, late September adventive root number 3-4 bar.
Be proof effect of the present invention, the spy does following five Comparative Examples.
Comparative Examples 1
Fresh wild Chinese podophyllum root seed, through cleaning, after the sorting be 0.5% potassium permanganate soaking at room temperature 2h sterilization with weight percent concentration, running water soaks cave dish sowing (type of seeding is coiled with embodiment 1 in the cave) behind the 46h.Seed begins before and after 54 days to germinate, and germination rate is about 5% before and after 80 days.
Comparative Examples 2:
The wild Chinese podophyllum root seed of gathering then, 4 ℃ of low temperature is husky to be hidden 2 months, and through cleaning, after the sorting being 0.5% potassium permanganate soaking at room temperature 2h sterilization with weight percent concentration, the cave dish was sowed (type of seeding is coiled with embodiment 1 in the cave) after running water soaked 46h.Seed begins before and after 47 days to germinate, and germination rate is about 35% before and after 80 days.
Comparative Examples 3
Fresh wild Chinese podophyllum root seed is through cleaning, after the sorting be 0.5% potassium permanganate soaking at room temperature 2h sterilization with weight percent concentration, 0.5% soaking through sodium carbonate 24h, cave dish sowing (type of seeding is coiled with embodiment 1 in the cave) behind the clear water bubble 22h.Begin before and after 50 days to germinate, germination rate is about 15% before and after 80 days.
Comparative Examples 4
The Chinese podophyllum root seedling that sprouts in the embodiment 1 described cave dish is not used HORMONE TREATMENT, from counting the day of sowing, begins to grow true leaf before and after 118 days, goes out the true leaf seedling and reaches 90% in about 163 days.May, early and middle ten days was transplanted the land for growing field crops then, took the shade net lucifuge, and 3 first quarter moons of grow are to early September, plant true leaf number 1-2 sheet (most of 1).
Comparative Examples 5
Gather then, the husky wild Chinese podophyllum root seeds of hiding 2 months of 4 ℃ of low temperature, after 30% saline solution sorting, running water is cleaned, do the ground late September drilling or broadcast sowing of growing seedlings of high furrow, take shed next year by the end of February, late April, seed began to germinate (7 months), late June germination rate reach about 35% (9 months); Mid or late July begins to grow true leaf (10 months), and it is about 31% that late September (12 months) goes out the true leaf seedling, seedling true leaf number 1-2 sheet (most 1), adventive root number 1-2 bar.
Comparative Examples 1-5 explanation, in the seedling raising process of Chinese podophyllum root, if adopt the conventional method different with patent of the present invention, seed begins sprout time and extends to 7 months, germination rate just reaches 35%, 10 month and grows rough leaf in the time of 9 months, goes out the true leaf seedling in the time of 12 months and has only 31%, therefore seedling raise period prolongs 8 months, and emergence rate reduces half; And seedling true leaf and adventive root number few (having only 1-2 sheet and 1-2 bar), the late growing stage of back seedling is transplanted in influence.
Claims (4)
1. medicinal plant Chinese podophyllum root artificial raise seedling method in imminent danger is characterized in that it may further comprise the steps:
A, presprouting of seeds: use wild Chinese podophyllum root seed, after sorting, dry surface moisture; The seed weight percent concentration is that 0.5% liquor potassic permanganate at room temperature soaks 2h, uses Gibberellins solution that concentration is 200mg/L, 4 ℃ again instead and soaks 46h; Remove the moisture of the surface of the seed, can sow;
B, cave dish sowing: humus soil is packed in the seedling culture hole plate, with concentration is that 50% carbendazol wettable powder is diluted with water to 800-1000 solution doubly, soil is 2 times in the dish of spray disinfectant cave, 2 seeds after the vernalization are sowed in every cave at random, degree of depth 2cm gets final product, earthing coils the cave short its sprouting in the environment that is positioned over temperature 17-22 ℃;
C, promote true leaf to take place and growth: waiting to germinate finish after, the continuous spraying concentration of cotyledon is the gibberellin 7 times of 200mg/L, totally 7 days once a day, root was executed the kinetin 3 times that concentration is 40mg/L then, executes 1 time in per 5 days; The growth of seedling environmental temperature is 17-22 ℃;
D, transplanting: above seedling is kept away the high light direct projection in early and middle ten days transplanting land for growing field crops in May.
2. medicinal plant Chinese podophyllum root artificial raise seedling method in imminent danger according to claim 1 is characterized in that: the fresh wild seed of the wild Chinese podophyllum root seed in the described steps A for gathering then.
3. medicinal plant Chinese podophyllum root artificial raise seedling method in imminent danger according to claim 1 is characterized in that: the wild Chinese podophyllum root seed in the described steps A is through at least 2 months wild Chinese podophyllum root seed of 4 ℃ of low temperature storages after the fresh then collection.
4. medicinal plant Chinese podophyllum root artificial raise seedling method in imminent danger according to claim 1 is characterized in that: in the described steps A wild Chinese podophyllum root seed separation being adopted weight percent concentration is the saline solution of 25%-30%.
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| CN201010578491XA CN102119621A (en) | 2010-12-08 | 2010-12-08 | Artificial seedling cultivation method of endangered medicinal plant Himalayan mayapple |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102763599A (en) * | 2012-08-20 | 2012-11-07 | 甘肃省科学院生物研究所 | Podophyllum hexandrum tissue culture seedling and exercising seedling transplanting acclimatization method |
| CN105309190A (en) * | 2015-11-20 | 2016-02-10 | 甘肃农业大学 | Winter seedling culture method for Tibetan herb podophyllum hexandrum Royle |
| CN105379535A (en) * | 2015-11-20 | 2016-03-09 | 甘肃农业大学 | Introduction and cultivation method for Tibetan medicine sinopodophyllumhexandrum |
| CN106489477A (en) * | 2016-10-12 | 2017-03-15 | 甘肃省科学院生物研究所 | A kind of Ecology Chinese podophyllum root method for culturing seedlings |
| CN107173001A (en) * | 2017-05-26 | 2017-09-19 | 香格里拉市雪域天赐电子商务有限公司 | The wild long column Smilacina japonica implantation methods of NW YUNNAN |
| CN107306626A (en) * | 2017-06-19 | 2017-11-03 | 香格里拉市忠浩野生中药种植有限责任公司 | A kind of method for culturing seedlings of Chinese podophyllum root |
| CN109042195A (en) * | 2018-09-27 | 2018-12-21 | 云南农业大学 | A kind of easy method for culturing seedlings of Chinese podophyllum root |
-
2010
- 2010-12-08 CN CN201010578491XA patent/CN102119621A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 《Biological Conservation》 20000131 M.Nadeem, L.M.S.Palni, A.N.Purohit, H.Pandey, S.K.Nandi Propagation and conservation of Podophyllum hexandrum Royle: an important medicinal herb 121-129 1-4 第92卷, 第1期 2 * |
| 《农业科学研究》 20100331 韩彩萍，余治家，樊亚鹏，胡永强，袁彩霞 六盘山野生濒危植物--桃儿七播种育苗技术研究 29-30,35 1-4 第31卷, 第1期 2 * |
| 《种子》 20080430 陈伟，杨楼，马绍宾 濒危药用植物桃儿七种子的萌发特性初探 49-51 1-4 第27卷, 第4期 2 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102763599A (en) * | 2012-08-20 | 2012-11-07 | 甘肃省科学院生物研究所 | Podophyllum hexandrum tissue culture seedling and exercising seedling transplanting acclimatization method |
| CN102763599B (en) * | 2012-08-20 | 2013-09-04 | 甘肃省科学院生物研究所 | Podophyllum hexandrum tissue culture seedling and exercising seedling transplanting acclimatization method |
| CN105309190A (en) * | 2015-11-20 | 2016-02-10 | 甘肃农业大学 | Winter seedling culture method for Tibetan herb podophyllum hexandrum Royle |
| CN105379535A (en) * | 2015-11-20 | 2016-03-09 | 甘肃农业大学 | Introduction and cultivation method for Tibetan medicine sinopodophyllumhexandrum |
| CN105379535B (en) * | 2015-11-20 | 2017-12-01 | 甘肃农业大学 | A kind of Tibetan medicine Chinese podophyllum root introduction and cultivation method |
| CN105309190B (en) * | 2015-11-20 | 2018-01-16 | 甘肃农业大学 | A kind of Tibetan medicine Chinese podophyllum root winter method for culturing seedlings |
| CN106489477A (en) * | 2016-10-12 | 2017-03-15 | 甘肃省科学院生物研究所 | A kind of Ecology Chinese podophyllum root method for culturing seedlings |
| CN106489477B (en) * | 2016-10-12 | 2019-10-25 | 甘肃省科学院生物研究所 | A kind of Ecology Chinese podophyllum root method for culturing seedlings |
| CN107173001A (en) * | 2017-05-26 | 2017-09-19 | 香格里拉市雪域天赐电子商务有限公司 | The wild long column Smilacina japonica implantation methods of NW YUNNAN |
| CN107306626A (en) * | 2017-06-19 | 2017-11-03 | 香格里拉市忠浩野生中药种植有限责任公司 | A kind of method for culturing seedlings of Chinese podophyllum root |
| CN109042195A (en) * | 2018-09-27 | 2018-12-21 | 云南农业大学 | A kind of easy method for culturing seedlings of Chinese podophyllum root |
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Application publication date: 20110713 |