CN105309190B - A kind of Tibetan medicine Chinese podophyllum root winter method for culturing seedlings - Google Patents
A kind of Tibetan medicine Chinese podophyllum root winter method for culturing seedlings Download PDFInfo
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- CN105309190B CN105309190B CN201510806053.7A CN201510806053A CN105309190B CN 105309190 B CN105309190 B CN 105309190B CN 201510806053 A CN201510806053 A CN 201510806053A CN 105309190 B CN105309190 B CN 105309190B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
A kind of Tibetan medicine Chinese podophyllum root winter seedling-raising technique, specific steps：A) the selection of seed；B) seedtime and environment；C) seed complete, that maturity is good, dry is immersed in the 5mol/L gibberellin aqueous solution 24~36 hours, sowing is in the seedling-growing container rich in humus soil, per basin 2 seeds, then 3~4cm of depth of planting will broadcast that seed-bearing seedling-growing container is neat to be placed in soil；D) seedling field management；E) seedling replanting：Autumn end Chinese podophyllum root aerial part turn yellow it is withered after, break seedling-growing container, by the Chinese podophyllum root root system with soil cultivate in 2200~3400m of height above sea level, 500~800mm of annual precipitation, translucency preferably, rich in the soil of humus.Percentage of seedgermination of the present invention is more than radical 2~3 when 96%, germinating energy is more than healthy and strong 85%, growth of seedling, transplanting, and root long reaches 18cm.
Description
Technical field
The invention belongs to Tibetan medicine Chinese podophyllum root seed seedling-raising technical field, specifically a kind of Tibetan medicine Chinese podophyllum root winter nursery
Method.
Background technology
Chinese podophyllum root (Podophyllum hexandrumRoyle) it is Berberidaceae（Berberidaceae）Sinopodophyllum
（Sinopodophyllum）Herbaceos perennial.It is distributed mainly on China Gansu, Qinghai, Shaanxi, Yunnan, Sichuan and Tibet
Etc. ground.Generally it is born in the m of height above sea level 1500~4300 flat mountain valley and the sylvan life of good transmittance, border or careless shrubbery；It is suitable to
Cold and moisten, Summer Low Temperature is rainy, the dry and cold weather of Winter-Spring.Research shows, podophyllotoxin content highest in Chinese podophyllum root rhizome,
Reach 2~10%.Modern medicine study shows that podophyllotoxin is a kind of lignans of viable commercial product, itself and its derivative
It is widely used in treatment condyloma acuminatum, anticancer with antitumor, anti-virus aspect.In addition, research is it is also shown that podophyllotoxin
Also have the function that to treat rheumatic arthritis, radioresistance, anti-oxidant etc..Lived because its is unique with significant pharmacology
Property, the extraction plant-- Chinese podophyllum root for causing people main to podophyllotoxin is excessively excavated for a long time and to face it in imminent danger.At present,
A large amount of podophyllotoxins are obtained by means such as chemical synthesis and biotechnologys to be still difficult to be commercialized.
Up to the present, study both at home and abroad in terms of the introduction and acclimatization cultivation of Podophyllum emodi var chinense class medicinal plant less.China is to peach
The nursery of youngster seven, artificial cultivation are at the early-stage, and only dormancy characteristics of different, germination percentage and germinating energy, planting density, nitrogen phosphorus proportion are applied
Primary Study has been carried out with influence to Chinese podophyllum root yield etc..Chinese podophyllum root seed is most, average seed number in each fruit
150.Due to the unique dormancy of Chinese podophyllum root seed and reproductive biology characteristic so that it is relatively low that survival rate is sprouted under natural conditions.
Therefore, it is necessary to utilize the most advantage of Chinese podophyllum root seed, according to its hibernation feature, grinding in terms of development Chinese podophyllum root seed seedling-raising
Study carefully, find the technologies such as suitable nursery approach, environment and field management, planted so as to accelerate the plantation of the large area of Tibetan medicine Chinese podophyllum root
Training and popularization, can not only meet wilderness demand of the market to podophyllotoxin, and may advantageously facilitate Chinese podophyllum root characteristic and provide naturally
The protection in source, utilize the sustainable development produced with podophyllotoxin.
The content of the invention
The technical problems to be solved by the invention are to provide one kind soon with promoting for Tibetan medicine Chinese podophyllum root large area growing and cultivation
Fast effective field method for culturing seedlings.
Used technical scheme is as follows：
A kind of Tibetan medicine Chinese podophyllum root winter method for culturing seedlings, is completed in the steps below：
(A) selection 1 of seed) the full ripe Chinese podophyllum root fruit of selection；2) seed strips out from fruit, clear with flowing water
Surface mucus is washed, is subsequently placed under illumination and dries；
(B) at the beginning of seedtime is the winter with environment seedtime, daily mean temperature selects height above sea level at -2 DEG C~4 DEG C
The preferable plot of 2200~3400m, 500~800mm of annual precipitation, translucency carries out nursery；
(C) sowing and seedling raising mannerses 1) by it is complete, maturity is good, the seed dried be soaked in 5.0 at 4 DEG C~
24~36 hours in the 8.0mol/L gibberellin aqueous solution；2) sowing is in the seedling-growing container rich in humus soil, per 2 kinds of basin
Son, 3~4cm of depth of planting；3) it will broadcast that seed-bearing seedling-growing container is neat to be placed in soil, seedling-growing container height and soil surface phase
It is flat；
(D) field management 1 of seedling) after spring temperature rise, the program request Broad Bean Seeds between seedling-growing container space；2) peach
After the germination of youngster seven, it is not necessary to weeding；
(E) transplanting of seedling breaks seedling-growing container after autumn end Chinese podophyllum root seedling aerial part turns yellow and withered, and will carry soil
The Chinese podophyllum root root system of earth cultivate in 2200~3400m of height above sea level, 500~800mm of annual precipitation, translucency preferably, rich in corruption
Grow in the soil of matter；Now Chinese podophyllum root seedling radical reaches 2-3 bars, and root long reaches 18cm.
Many researchs show that it is to cause the main original of Chinese podophyllum root seed dormancy to plant skin and endosperm limitation and physiology after-ripening
Cause.Winter nursery is selected, coordinates the Gibberellins solution processing of 5.0~8.0mol/L concentration, the physiological dormancy of Chinese podophyllum root seed exists
Released completely under conditions of natural low temperature and exogenous hormone so that its germination percentage and germinating energy are higher；In addition, favourable life
State environment promoted spring germinate after seedling grow.So the present invention gives birth to according to Tibetan medicine Chinese podophyllum root seed dormancy and breeding
Thing characteristic carries out field nursery, and it has：(A) germination percentage and germinating energy usury are more than with this method for culturing seedlings, percentage of seedgermination
96%th, germinating energy is more than 85%；(B) radical 2~3 when the good growth of seedling stalwartness of Seedling potential, transplanting, root long reaches 18cm
The characteristics of.
The present invention not only contributes to the large area growing and cultivation of Tibetan medicine Chinese podophyllum root with promoting, also to protecting wild Chinese podophyllum root certainly
Right resource is significant.
Embodiment
The present invention is made below by the Tibetan medicine Chinese podophyllum root winter seedling raising process done in Gansu Province Tibetan Autonomous Prefecture of Gannan
It is further described.
A kind of Tibetan medicine Chinese podophyllum root winter method for culturing seedlings, is completed in the steps below：
(A) selection 1 of seed) the full ripe peach that grows to the collection Gansu Province Xiahe County Bo La townshiies mountain forest of September 2 days
The fruit of youngster seven；2) seed is stripped out from fruit, and surface mucus is cleaned with flowing water, is subsequently placed under illumination and is dried.
(B) seedtime and environment seedtime are November 15, Gansu Province Tibetan Autonomous Prefecture of Gannan agriculture Hezuo City agriculture section
Institute's Experimental Base daily mean temperature is at 0.5 DEG C（Can also be at -2 DEG C, -1 DEG C, 1 DEG C, 2 DEG C, 3 DEG C or 4 DEG C）When, height above sea level
2863m, 600~800mm of annual precipitation, face south, the soil environment rich in humus.
(C) sowing with seedling raising mannerses 1) by it is complete, maturity is good, dry Chinese podophyllum root seed under the conditions of 4 DEG C, immersion
In 5.0mol/L, 6.0mol/L, 7.0mol/L or 8.0mol/L gibberellin（GA3）24 in the aqueous solution, 25,26,27,28,29,
30th, 31,32,33,34,35 or 36 hours；2) sowing rich in humus soil, diameter 6cm, height 10cm seedling-growing container in,
2 seeds, depth of planting 3cm, 3.5cm or 4cm are sowed per basin；3) seed-bearing seedling-growing container will be broadcast fitly to be placed in soil,
Space is filled with soil, and seedling-growing container height is equal with soil surface.
(D) field management 1 of seedling) after spring May 3 daily temperature go up after, the program request silkworm in soil between seedling-growing container space
Beans seed, density is 0.5m × 0.5m, and in bean plant growth and development process, its main function is that Chinese podophyllum root seedling is played
The effect of shade；2) after Chinese podophyllum root germination, field does not need weeding.
(E) transplanting 1 of seedling) in autumn Mo next year, Chinese podophyllum root seedling aerial part turn yellow it is withered after, now radical is reachable
To 2-3 bars, root long reaches 18cm；2) seedling-growing container is broken, the Chinese podophyllum root root system with soil is transplanted in above-mentioned Gansu Province Gannan
Tibetan autonomous prefecture agriculture Hezuo City institute of agricultural sciences Experimental Base.
Claims (1)
- A kind of 1. Tibetan medicine Chinese podophyllum root winter method for culturing seedlings, it is characterised in that：Complete in the steps below：(A) selection 1 of seed) the full ripe Chinese podophyllum root fruit of selection；2) seed is stripped out from fruit, and table is cleaned with flowing water Face mucus, it is subsequently placed under illumination and dries；(B) at the beginning of seedtime is the winter with environment seedtime, daily mean temperature selects height above sea level 2200 at -2 DEG C~4 DEG C The preferable plot of~3400m, 500~800mm of annual precipitation, translucency carries out nursery；(C) sow and seedling raising mannerses 1) seed complete, that maturity is good, dry is soaked in 5.0~8.0mol/L at 4 DEG C The gibberellin aqueous solution in 24~36 hours；2) sowing is in the seedling-growing container rich in humus soil, per 2 seeds of basin, sowing 3~4cm of depth；3) it will broadcast that seed-bearing seedling-growing container is neat to be placed in soil, seedling-growing container height is equal with soil surface；(D) field management 1 of seedling) after spring temperature rise, the program request Broad Bean Seeds between seedling-growing container space；2) Chinese podophyllum root After germination, it is not necessary to weeding；(E) transplanting of seedling breaks seedling-growing container, by with soil after autumn end Chinese podophyllum root seedling aerial part turns yellow and withered Chinese podophyllum root root system cultivate in 2200~3400m of height above sea level, 500~800mm of annual precipitation, translucency preferably, rich in humus Soil in；Now Chinese podophyllum root seedling radical reaches 2-3 bars, and root long reaches 18cm.
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| CN105309190B true CN105309190B (en) | 2018-01-16 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489477B (en) * | 2016-10-12 | 2019-10-25 | 甘肃省科学院生物研究所 | A kind of Ecology Chinese podophyllum root method for culturing seedlings |
| CN109042195A (en) * | 2018-09-27 | 2018-12-21 | 云南农业大学 | A kind of easy method for culturing seedlings of Chinese podophyllum root |
| CN111713403B (en) * | 2020-07-31 | 2022-02-18 | 金苑(北京)农业技术研究院有限公司 | Method for doubling corn haploid seedlings |
| CN115735665A (en) * | 2022-12-20 | 2023-03-07 | 甘肃本草元中药材有限公司 | Honeysuckle seedling cultivation method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102119621A (en) * | 2010-12-08 | 2011-07-13 | 甘肃省科学院生物研究所 | Artificial seedling cultivation method of endangered medicinal plant Himalayan mayapple |
| CN102763599A (en) * | 2012-08-20 | 2012-11-07 | 甘肃省科学院生物研究所 | Podophyllum hexandrum tissue culture seedling and exercising seedling transplanting acclimatization method |
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2015
- 2015-11-20 CN CN201510806053.7A patent/CN105309190B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102119621A (en) * | 2010-12-08 | 2011-07-13 | 甘肃省科学院生物研究所 | Artificial seedling cultivation method of endangered medicinal plant Himalayan mayapple |
| CN102763599A (en) * | 2012-08-20 | 2012-11-07 | 甘肃省科学院生物研究所 | Podophyllum hexandrum tissue culture seedling and exercising seedling transplanting acclimatization method |
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| 六盘山野生濒危物种—桃儿七的繁育技术研究;王再斌等;《陕西农业科学》;20080925(第5期);第115~116,186页 * |
| 桃儿七种子休眠与萌发特性的研究;李城德等;《中国种业》;20080215(第2期);第52~53页 * |
| 濒危药用植物桃儿七种子的萌发特性初探;陈伟等;《种子》;20080425(第4期);第49~51页 * |
| 西藏地区玉米行间作桃儿七模式化栽培技术;王军峰等;《现代农业科技》;20140325(第23期);第128,131页 * |
| 西藏野生桃儿七生物学特性及人工栽培技术研究;鲍隆友等;《中国林副特产》;20040820(第4期);第1~2页 * |
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Inventor after: Li Mengfei Inventor after: Ma Zhanjun Inventor after: Ding Yaolu Inventor after: Ge Li Inventor after: Yang Delong Inventor after: Wang Bohui Inventor after: Li Wei Inventor before: Li Mengfei Inventor before: Ding Yaolu Inventor before: Ge Li Inventor before: Yang Delong Inventor before: Wang Bohui Inventor before: Li Wei |
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Effective date of registration: 20191108 Address after: Shop on the first floor of building 6, Beichen Anxing, Oushi street, Ganzhou District, Zhangye City, Gansu Province Patentee after: ZHANGYE BOFENG AGRICULTURAL SCIENCE AND TECHNOLOGY CO., LTD. Address before: 730070 No. 1 village gate, Anning District, Gansu, Lanzhou Patentee before: Gansu Agricultural University |
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