CN107306626A - A kind of method for culturing seedlings of Chinese podophyllum root - Google Patents
A kind of method for culturing seedlings of Chinese podophyllum root Download PDFInfo
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- CN107306626A CN107306626A CN201710464758.4A CN201710464758A CN107306626A CN 107306626 A CN107306626 A CN 107306626A CN 201710464758 A CN201710464758 A CN 201710464758A CN 107306626 A CN107306626 A CN 107306626A
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- chinese podophyllum
- podophyllum root
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- Soil Sciences (AREA)
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Abstract
The present invention relates to planting technical field, a kind of specifically method for culturing seedlings of Chinese podophyllum root;The technical scheme of use includes seed collection → seed treatment → nursery selection → site preparation → sowing → field management → transplanting;The present invention breaks Chinese podophyllum root seed dormancy by carrying out low temperature Sand culture to Chinese podophyllum root seed, improves percentage of seedgermination, the Chinese podophyllum root percentage of seedgermination handled by this method is up to more than 95%, and Chinese podophyllum root seedling percent reaches more than 90%.
Description
Technical field
The present invention relates to planting technical field, a kind of specifically method for culturing seedlings of Chinese podophyllum root.
Background technology
Chinese podophyllum root (Sinopodophyllum hexandrum) is Podophylloideae Sinopodophyllum, a kind of perennial
Herbaceous plant, is distributed in the Yunnan, Sichuan, Shaanxi, Gansu, Tibet, Hubei of China, the ground such as India, Nepal abroad
It is distributed.Its root, rhizome, leaf and fruit are traditional herbal medicines, are the medicinal plants with long applicating history, grinding in recent years
Study carefully and show there is the higher podophyllotoxin of content (Podophyllotoxin) in Chinese podophyllum root root and rhizome, be that synthesis VP16 (relies on pool
Glycosides) and a variety of cancer therapy drugs such as VM26 (Teniposide) precursor, while being also the head for treating cutaneum carcinoma and sexually transmitted disease condyloma
Select medicine.Chinese podophyllum root is medicinal at present, and one is that under ground portion (Podophyllum emodi var chinense) is used to extract podophyllotoxin (synthesis cancer therapy drug VP-16/
The precursor component of Etoposide, VM-26/ Teniposides), it is " day lucky star " III anticarcinogens that main ingredient is made with " Chinese podophyllum root ",
Therapeutic effect for breast cancer is particularly evident, and clinical effective rate is fruit as Tibetan medicine material up to 88.5%, two, is claimed " Himalayan mayapple fruit "
(Tibetan's language claims " Ao Mosai "), for various gynecological diseases, also there is the custom of its edible fruit in Tibetan.Chinese podophyllum root is in China master
It is distributed in the Mount Taibai area in Shaanxi, the county such as Tianzhu Area, Gansu and political affairs, Jone, Wen County, western Sichuan, Yunnan Deqie, Shangri-la
Etc. ground, the ground such as Tibet Changdu, Linzhi is generally born in height above sea level l500~4300m high mountain thick grass or woods side, moistened suitable for cold
Ecological environment.
In recent years, its under ground portion is largely on the one hand excavated due to the place of production and extracts podophyllotoxin, cause wild resource amount sharp
Subtract and fit raw environmental destruction, its fruit of another aspect Tibetan medicine and pharmacology also directly affects the natural renovation of its resource, and at present
Large-scale cultivation production is there is no, Chinese podophyllum root population quantity is just drastically less, and asexual and generative propagation ability weakens significantly, resource
Condition in imminent danger is faced, has been put into《Chinese rare or endangered species register》, and by《Chinese Plants Red Data Book》Include, be three-level
Protect plant.
It is therefore desirable to study the whole growth phase of Chinese podophyllum root, artificial culture mode is taken, Chinese podophyllum root is improved
Seedling setting ratio research.
The content of the invention
It is an object of the invention to provide a kind of nursery for the Chinese podophyllum root for increasing Chinese podophyllum root percentage of seedgermination and seedling percent
Method.
To achieve the above object, the technical solution adopted by the present invention is:A kind of method for culturing seedlings of Chinese podophyllum root, in the steps below
Complete:
(1) seed collection:In mid-August to Chinese podophyllum root fruit full maturity by the end of September, is treated, harvested when fruit is red, fruit
Removed the peel after real harvesting, the pulp on seed is cleaned by clear water, remove not full seed, the moisture for drying the surface of the seed is standby;
(2) seed treatment:Seed will be dried described in above-mentioned steps (1) to be soaked 2 hours with 0.5% liquor potassic permanganate,
Cleaned up with clear water;Then seed and river sand are mixed, the mixture mixed is loaded into mesh bag, mesh bag width of mesh is to fill
Enter river sand and do not spill to be advisable, select a permeable good plot to dig pit and be buried in the earth the mesh bag equipped with mixture, between mesh bag
Tamped with soil, mesh bag top can be sowed covered with soil layer when seed shows money or valuables one carries unintentionally Second Year by the end of March;
(3) nursery is selected:Select high altitude localities draining good, loosing soil, the plot rich in humus;
(4) site preparation:Above-mentioned nursery plot is deep ploughed, while applying base manure, and soil disinfection is carried out, is flattened after deep ploughing
Rake is thin, does furrow, wide 1 meter, furrow are high 10~15 centimetres;
(5) sow:Sowing time selection the Ching Ming Festival front and rear 5 days, it is during sowing that the seed showed money or valuables one carries unintentionally is public by every mu 45~55
Jin sowing quantity, uniform broadcasting is in furrow face, then covers the fine earth of 1~1.5 cm thick, and is capped the pine needle of one layer of 5 cm, fits
When watering, shade density is built on seedbed for 70% shade, canopy is high 1.8 meters, and Chinese podophyllum root starts to emerge for after planting 15 days, 30
It or so all sprouts substantially, during which notes progressively removing the pine needle of Chinese podophyllum root seedling top covering, is removed in three times before and after selection sunset
The pine needle of covering, removes about 1/3, every minor tick 2 days will be sprayed water after removing pine needle every time;
(6) field management
A weedings:Chinese podophyllum root young plant weeding, based on artificial uprooting weed, has just been sprouted in weeds, and root system is pulled out in time when not yet length is opened
Remove, accomplish to see that grass is just removed, reduce the injury to Chinese podophyllum root seedlings root;
B water and fertilizer managements:Water based on sprinkling irrigation, to keep seedbed to moisten, ponding is not advisable, the later stage sprays phosphorus with reference to sprinkling irrigation
Acid dihydride potassium is topdressed;
The C prevention and control of plant diseases, pest control:The pest and disease damage of Chinese podophyllum root Seedling Stage is more rare, mainly aphid damage, finds aphid damage
When, it can be sprayed and prevented and treated with the solution of 40% flolimat emulsion 0.067%;
(7) transplant:The field-transplanting of Chinese podophyllum root Second Year early spring after nursery is carried out, and selection bud is carried out before sprouting, and is transplanted
It is preceding that nursery is first poured permeable, to avoid hurting seedling root and bud when excavating seedling.
Further:Seed and river sand are according to 1 in step (2) seed treatment:3 ratio mixing, the river sand contains
Water is 25%~35%, and the mesh bag top overlying soil thickness is 15~25cm.
Further:3207 meters of Shangri-la little Zhong Dian height above sea level, annual gas are selected during step (3) the nursery selection
5.8 DEG C of temperature, 849.8 millimeters of annual rainfall, 120 days frost-free periods, draining is good, loosing soil, the plot rich in humus.
Further:It is every mu using base manure consumption during step (4) site preparation and uses well-rotted farmyard manure 2500kg and multiple
Hefei 40kg soil disinfections are diluted using 1200 times of liquid of 70% wettable powder of Bravo, 600 times of liquid and thiophanate methyl.
The method have the benefit that:This method breaks peach by carrying out low temperature Sand culture to Chinese podophyllum root seed
The seed dormancy of youngster seven, improves percentage of seedgermination, the Chinese podophyllum root percentage of seedgermination handled by this method is up to 100%, Chinese podophyllum root
Seedling percent is up to more than 95%;Simultaneously by the research to Chinese podophyllum root growth cycle and breeding experiment, a whole set of has been formulated
Chinese podophyllum root seedling management method, scientific management is greatly enhanced Chinese podophyllum root seedling percent.
Embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, this area is common
The every other embodiment that technical staff is obtained under the premise of creative work is not made, belongs to the model that the present invention is protected
Enclose.
Embodiment 1:
A kind of method for culturing seedlings of Chinese podophyllum root, is completed in the steps below:
(1) seed collection:Typically adopted in mid-August to Chinese podophyllum root fruit full maturity by the end of September, is treated when fruit is red
Receive, removed the peel after fruit harvesting, the pulp on seed is cleaned by clear water, remove not full seed, dry the moisture of the surface of the seed
It is standby;
(2) seed treatment:The Chinese podophyllum root seed that the moisture of the surface of the seed will be dried soaks 2 with 0.5% liquor potassic permanganate
Hour, cleaned up with clear water, by seed and river sand 1:3 ratio mixing, the river sand water content is 30% or so, will be mixed
Good mixture loads mesh bag, and mesh bag width of mesh is not spilt with to load river sand and is advisable, select a permeable good plot dig pit by
The mesh bag is buried in the earth, and is tamped between mesh bag with soil, and the soil layer of one layer of 20 cm thick is covered above, Second Year seed by the end of March is treated
It can be sowed when showing money or valuables one carries unintentionally;Chinese podophyllum root seed has its rest period under stronger hibernation feature, natural conditions to be 10 months, we
Method can effectively break the dormancy of seed using the method for low temperature sand storage;
(3) nursery is selected:Select the loyal great wild small Zhong Dian planting bases of Chinese medicine plant Co., Ltd in Xianggelila country, sea
Pull out 3207 meters, 5.8 DEG C of average temperature of the whole year, 849.8 millimeters of annual rainfall, 120 days frost-free periods, soil loose, rich in humus
Environment;
(4) site preparation:By above-mentioned selection draining is good, the soil is porous, fertile plot, is deep ploughed, applied with reference to deep ploughing
Base manure, every mu makes well-rotted farmyard manure 2500kg and composite fertilizer 40kg, and the composite fertilizer is the common composite fertilizer that in the market is bought, its
Micronutrient levels ratio is N:P:K contents are 15:15:15, and with 70% wettable powder of Bravo, 600 times of liquid and methyl support
The liquid dilution of 1200 times of cloth Tianjin carries out soil disinfection, and leveling rake is thin after deep ploughing, does furrow, wide 1 meter, long to be determined with ground block length, and furrow are high
10~15 centimetres;
(5) sow:It is during sowing that the seed showed money or valuables one carries unintentionally is public by every mu 50 front and rear 5 days the Ching Ming Festival sowing time is typically chosen in
Jin sowing quantity, uniform broadcasting is in furrow face, then covers the fine earth of 1~1.5 cm thick, and is capped one layer about 5 centimetres of pine needle, keeps
The temperature and humidity in seedbed, beneficial to the sprouting of Chinese podophyllum root seed, are suitably watered afterwards, and shade density is built on seedbed and is hidden for 70%
Cloudy canopy, canopy is high 1.8 meters, and shade main function causes seedling to burn to avoid the straight Sai Chinese podophyllum roots seedling of the sun, influence seedling into
Living, Chinese podophyllum root starts to emerge, all sprouted substantially within 30 days or so for after planting 15 days, and Chinese podophyllum root starts that the pine of covering should be removed after emerging
Pin, removes the pine needle of covering in three times before and after selection sunset, about 1/3 is removed every time, every minor tick 2 days removes pine needle effect one
It is to avoid the column seedling caused by humidity is too high, two be to avoid the Chinese podophyllum root seedling deformity caused by covering pine needle, to be entered after removing pine needle
Row water spray;
(6) field management
A weedings:Chinese podophyllum root young plant weeding, based on artificial uprooting weed, pulls out in weeds Seedling Stage, has just been sprouted in weeds, root
System pulls out in time when not yet length is opened, and accomplishes to see that grass is just removed, reduces the injury to Chinese podophyllum root seedlings root;
B water and fertilizer managements:Water based on sprinkling irrigation, to keep seedbed to moisten, ponding is not advisable, the later stage sprays phosphorus with reference to sprinkling irrigation
Acid dihydride potassium is topdressed;
The C prevention and control of plant diseases, pest control:The pest and disease damage of Chinese podophyllum root Seedling Stage is more rare, common aphid damage, and aphid is in Chinese podophyllum root
Whole seedling stage can occurrence injury, be particularly the peak period occurred in arid season, main harm blade causes blade when serious
Flavescence comes off, and causes Chinese podophyllum root death of seedling;It was found that during aphid damage, can be sprayed with the solution of 40% flolimat emulsion 0.067%
Mist is prevented and treated, and preventive effect is up to more than 95%;
(7) transplant:The field-transplanting of Chinese podophyllum root Second Year early spring after nursery is carried out, and is typically chosen before bud is sprouted and is carried out,
Nursery is first poured permeable before transplanting, avoids hurting seedling root and bud when excavating seedling.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God is with principle, and any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (4)
1. a kind of method for culturing seedlings of Chinese podophyllum root, it is characterised in that:Complete in the steps below:
(1) seed collection:In mid-August to Chinese podophyllum root fruit full maturity by the end of September, is treated, harvested when fruit is red, fruit is adopted
Removed the peel after receipts, the pulp on seed is cleaned by clear water, remove not full seed, the moisture for drying the surface of the seed is standby;
(2) seed treatment:Seed will be dried described in above-mentioned steps (1) to be soaked 2 hours with 0.5% liquor potassic permanganate, with clear
Water is cleaned up;Then seed and river sand are mixed, the mixture mixed is loaded into mesh bag, mesh bag width of mesh is to load river
Sand, which is not spilt, to be advisable, and selects a permeable good plot to dig pit and is buried in the earth the mesh bag equipped with mixture, with soil between mesh bag
Tamp, mesh bag top can be sowed covered with soil layer when seed shows money or valuables one carries unintentionally Second Year by the end of March;
(3) nursery is selected:Select high altitude localities draining good, loosing soil, the plot rich in humus;
(4) site preparation:Above-mentioned nursery plot is deep ploughed, while applying base manure, and soil disinfection is carried out, rake is flattened after deep ploughing
Carefully, furrow are done, wide 1 meter, furrow are high 10~15 centimetres;
(5) sow:Sowing time selection the Ching Ming Festival front and rear 5 days, during sowing by the seed showed money or valuables one carries unintentionally by 45~55 kilograms every mu with
Amount is planted, uniform broadcasting is in furrow face, then covers the fine earth of 1~1.5 cm thick, and is capped the pine needle of one layer of 5 cm, suitably pours
Water, builds shade density for 70% shade, canopy is high 1.8 meters, and Chinese podophyllum root starts to emerge for after planting 15 days, 30 days or so on seedbed
Substantially all sprout, during which note progressively removing the pine needle of Chinese podophyllum root seedling top covering, covering is removed in three times before and after selection sunset
Pine needle, removes about 1/3, every minor tick 2 days will be sprayed water after removing pine needle every time;
(6) field management
A weedings:Chinese podophyllum root young plant weeding, based on artificial uprooting weed, has just been sprouted in weeds, and root system is pulled out in time when not yet length is opened,
Accomplish to see that grass is just removed, reduce the injury to Chinese podophyllum root seedlings root;
B water and fertilizer managements:Water based on sprinkling irrigation, to keep seedbed to moisten, ponding is not advisable, the later stage sprays di(2-ethylhexyl)phosphate with reference to sprinkling irrigation
Hydrogen potassium is topdressed;
The C prevention and control of plant diseases, pest control:The pest and disease damage of Chinese podophyllum root Seedling Stage is more rare, mainly aphid damage, can when finding aphid damage
Sprayed and prevented and treated with the solution of 40% flolimat emulsion 0.067%;
(7) transplant:The field-transplanting of Chinese podophyllum root Second Year early spring after nursery is carried out, and selection bud is carried out before sprouting, before transplanting first
Nursery is poured permeable, to avoid hurting seedling root and bud when excavating seedling.
2. a kind of method for culturing seedlings of Chinese podophyllum root according to claim 1, it is characterised in that:Step (2) seed treatment
Middle seed and river sand are according to 1:3 ratio mixing, the river sand water content is 25%~35%, mesh bag top overlying soil
Thickness is 15~25cm.
3. a kind of method for culturing seedlings of Chinese podophyllum root according to claim 1, it is characterised in that:Step (3) the nursery choosing
3207 meters of Shangri-la little Zhong Dian height above sea level, 5.8 DEG C of average temperature of the whole year, 849.8 millimeters of annual rainfall, frost-free period 120 are selected when selecting
My god, draining is good, loosing soil, the plot rich in humus.
4. a kind of method for culturing seedlings of Chinese podophyllum root according to claim 1, it is characterised in that:Make during step (4) site preparation
It is every mu with base manure consumption and uses the wettable of Bravo 70% using well-rotted farmyard manure 2500kg and composite fertilizer's 40kg soil disinfections
1200 times of liquid of 600 times of liquid of pulvis and thiophanate methyl dilute.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109042195A (en) * | 2018-09-27 | 2018-12-21 | 云南农业大学 | A kind of easy method for culturing seedlings of Chinese podophyllum root |
CN109496657A (en) * | 2018-12-10 | 2019-03-22 | 怀化正好制药有限公司 | The method of callicarpa kwangtungensis Chun nursery |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109042195A (en) * | 2018-09-27 | 2018-12-21 | 云南农业大学 | A kind of easy method for culturing seedlings of Chinese podophyllum root |
CN109496657A (en) * | 2018-12-10 | 2019-03-22 | 怀化正好制药有限公司 | The method of callicarpa kwangtungensis Chun nursery |
CN109496657B (en) * | 2018-12-10 | 2021-04-06 | 怀化正好制药有限公司 | Callicarpa kwangtungensis seedling culture method |
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