CN104054427A - Method for enhancing germination rate of fimbristylis dichotoma seed - Google Patents
Method for enhancing germination rate of fimbristylis dichotoma seed Download PDFInfo
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- CN104054427A CN104054427A CN201410247321.1A CN201410247321A CN104054427A CN 104054427 A CN104054427 A CN 104054427A CN 201410247321 A CN201410247321 A CN 201410247321A CN 104054427 A CN104054427 A CN 104054427A
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Abstract
The invention discloses a method for enhancing the germination rate of fimbristylis dichotoma seeds. The method comprises the following steps of: A, manually cleaning the fimbristylis dichotoma seeds, and removing impurities; B, loading the fimbristylis dichotoma seeds into a single-layer gauze bag, and frapping a bag opening by using a rubber band; C, placing the single-layer gauze bag into an alkali solution, and carrying out seed soaking at room temperature; D, taking the single-layer gauze bag out of the alkali solution, completely throwing the attached alkali solution, then placing the single-layer gauze bag into distilled water to soak for 20 minutes, then washing for three times by using the distilled water, taking the fimbristylis dichotoma seeds out of the single-layer gauze bag, and uniformly scattering into a culture dish with two layers of filter papers paved; E, placing the culture dish into a 25 DEG C illumination culture box for 3-5 days, and keeping the filter papers wet by frequently spraying water in the meantime. The method for enhancing the germination rate of the fimbristylis dichotoma seeds, which is provided by the invention, is simple, easy and convenient to operate and shortened in time and can break the dormancy of the fimbristylis dichotoma seeds, promote the germination of the fimbristylis dichotoma seeds and effectively enhance the germination of the seeds of cyperaceae plants, such as the fimbristylis dichotoma.
Description
Technical field
The present invention relates to a kind of method that improves percentage of seedgermination, be specifically related to a kind of method that improves dichotomous fimbristylis herb percentage of seedgermination.
Background technology
Not tally dichotomous fimbristylis herb is sedge family genus fimbristylis plant, and not only herb can be used as medicine, and heavy metal cadmium contaminated soil is had to the effect of absorption cleaning.Not tally dichotomous fimbristylis herb (Fimbristylis dichotoma (L) Vahl) is annual herb.Stalk is grown thickly, high 20-50cm.Complete stool is without hair or have thin pubescence.Leaf is linear, wide 1-2.5mm, the anxious point of tip or blunt; Sheath base portion keratin.Lobate bract 3-4 under inflorescence, normal 1-2 sheet, is longer than inflorescence.Cyme is appeared again or simply; Small ear is avette or oval shape is avette, long 5-12mm, and wide 2-3mm, has most flowers; Avette or the Long Circle of scale, long 2-2.5mm, sepia, glossy, there is 3-5 arteries and veins, tip has short point; Stamen 2-3; Style is flat, and echinid is arranged at top; Column cap 2.The wide obovate of nutlet, biconvex shape, long 1-1.2mm, white is to filbert, the surface circular reticulate pattern of growing crosswise, vertical rib 7-9 bar, significantly protuberance, has brown short handle.
Not tally dichotomous fimbristylis herb is as a kind of medicinal plant and heavy metal adsorption plant, the general seminal propagation method of using, there is establishing in large scale though existing, but there is dormancy in the seed of not tally dichotomous fimbristylis herb, the percentage of seedgermination of clear water processing only only has 5-7 grain seed sprouting in 5%-7%(100 grain seed), and conventional hot-water soak germinating method also can only be brought up to germination rate 37-50%, affect exploitation and the spread of dichotomous fimbristylis herb.The germination rate that how to improve dichotomous fimbristylis herb is the matter of utmost importance that needs solution in production at present and environmental protection.
Summary of the invention
Problem to be solved by this invention is, for the deficiency of above-mentioned technology, to design a kind of method that improves dichotomous fimbristylis herb percentage of seedgermination.
The present invention for the technical scheme that its problem of solution adopts is:
A method that improves dichotomous fimbristylis herb percentage of seedgermination, comprises the following steps:
A, dichotomous fimbristylis herb seed is manually cleaned, impurity is removed;
B, pack: dichotomous fimbristylis herb seed is packed in individual layer gauze bag, and sack is tightened with rubber band;
C, seed soaking: individual layer gauze bag is put into aqueous slkali, at room temperature soak seed;
D, flushing: individual layer gauze bag is taken out from aqueous slkali, and dry the aqueous slkali that only attaches, then put it into and in distilled water, soak 20min, and then with distilled water flushing 3 times, dichotomous fimbristylis herb seed is evenly spilt in the culture dish that is covered with 2 metafiltration paper from taking out in individual layer gauze bag;
E, cultivation: culture dish is put into 25 DEG C of illumination box 3-5 days, often need during this period water spray to make filter paper keep moistening.
In C step of the present invention, the sodium hydroxide solution that the concentration of described aqueous slkali is 5%-80%, the processing time of seed soaking is 5min-2h.
In C step of the present invention, the sodium hydroxide solution that the concentration of described aqueous slkali is 20%, the processing time of seed soaking is 40min.
The invention has the beneficial effects as follows: the present invention is applied to the germination rate that improves seed, adopt the method for raising percentage of seedgermination provided by the invention, the dormancy that can break seed, promotes its sprouting.Meanwhile, the inventive method is simple, easy and simple to handle, time shorten, can effectively improve the germination of the sedge seeds such as similar dichotomous fimbristylis herb by the method.
In addition, the inventive method by cleaning, pack, soak seed, flushing, incubation step processing, make dichotomous fimbristylis herb seed have higher germination rate, improved 30-50% than conventional seed germinating method, have obvious advantage.Therefore adopt the present invention to improve the method for dichotomous fimbristylis herb seed sprouting, can effectively promote the germination of dichotomous fimbristylis herb seed.
Embodiment
Below with reference to embodiment, the technique effect of design of the present invention, generation is carried out to clear, complete description, to understand fully object of the present invention, feature and effect.Obviously; described embodiment is a part of embodiment of the present invention, instead of whole embodiment, based on embodiments of the invention; other embodiment that those skilled in the art obtains under the prerequisite of not paying creative work, all belong to the scope of protection of the invention.
A kind of method that improves percentage of seedgermination provided by the present invention, comprises the following steps:
A, clean: dichotomous fimbristylis herb seed is manually cleaned, impurity is removed;
B, pack: dichotomous fimbristylis herb seed is packed in individual layer gauze bag, and sack is tightened with rubber band;
C, seed soaking: individual layer gauze bag is put into aqueous slkali, at room temperature soak seed;
D, flushing: individual layer gauze bag is taken out from aqueous slkali, and dry the aqueous slkali that only attaches, then put it into and in distilled water, soak 20min, and then with distilled water flushing 3 times, dichotomous fimbristylis herb seed is evenly spilt in the culture dish that is covered with 2 metafiltration paper from taking out in individual layer gauze bag;
E, cultivation: culture dish is put into 25 DEG C of illumination box 3-5 days, often need during this period water spray to make filter paper keep moistening.
Further, in C step, the sodium hydroxide solution that the concentration of described aqueous slkali is 5%-80%, the processing time of seed soaking is 5min-2h.
As preferred embodiment one, in C step, the sodium hydroxide solution that the concentration of described aqueous slkali is 20%, the processing time of seed soaking is 40min.Will be according to this parameter value dichotomous fimbristylis herb planting seed after treatment, sowing area is herbage base, Village, treasured island, Danzhou, Hainan, be positioned at 109 ° 30 of east longitude ', 19 ° 30 of north latitude ', belong to tropical monsoon climate, year-round average temperature is 25.0 DEG C of left and right, and annual rainfall is 2052.0mm, and a year active accumulated temperature is 8652.0 DEG C; Its cultivating soil type is the brick red loam that granite is grown, and the plow layer that soil pH is 5.5,0-20cm is containing organic 1.4%.Reach 90% at the germination rate of this parameter value dichotomous fimbristylis herb seed after treatment, seed germination coefficient is up to 18.00.
As preferred embodiment two to five:
| Embodiment | Two | Three | Four | Five |
| Concentration of sodium hydroxide solution (%) | 5 | 10 | 40 | 80 |
| The sodium hydroxide solution processing time | 2h | 1h | 20min | 5min |
Undertaken by above-mentioned parameter, remaining operating procedure, planting environment are all identical with embodiment one.
Can reach 70-85%, seed germination coefficient at 12.25-16.00 at the germination rate of above-mentioned parameter value dichotomous fimbristylis herb seed after treatment.
Above preferred embodiments of the present invention is illustrated, but the present invention is not limited to described embodiment, those of ordinary skill in the art also can make all equivalent modifications or replacement under the prerequisite without prejudice to spirit of the present invention, and the modification that these are equal to or replacement are all included in the application's claim limited range.
Claims (3)
1. improve a method for dichotomous fimbristylis herb percentage of seedgermination, it is characterized in that comprising the following steps:
A, clean: dichotomous fimbristylis herb seed is manually cleaned, impurity is removed;
B, pack: dichotomous fimbristylis herb seed is packed in individual layer gauze bag, and sack is tightened with rubber band;
C, seed soaking: individual layer gauze bag is put into aqueous slkali, at room temperature soak seed;
D, flushing: individual layer gauze bag is taken out from aqueous slkali, and dry the aqueous slkali that only attaches, then put it into and in distilled water, soak 20min, and then with distilled water flushing 3 times, dichotomous fimbristylis herb seed is evenly spilt in the culture dish that is covered with 2 metafiltration paper from taking out in individual layer gauze bag;
E, cultivation: culture dish is put into 25 DEG C of illumination box 3-5 days, often need during this period water spray to make filter paper keep moistening.
2. a kind of method that improves dichotomous fimbristylis herb percentage of seedgermination according to claim 1, is characterized in that: in C step, and the sodium hydroxide solution that the concentration of described aqueous slkali is 5%-80%, the processing time of seed soaking is 5min-2h.
3. a kind of method that improves dichotomous fimbristylis herb percentage of seedgermination according to claim 2, is characterized in that: in C step, and the sodium hydroxide solution that the concentration of described aqueous slkali is 20%, the processing time of seed soaking is 40min.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410247321.1A CN104054427B (en) | 2014-06-06 | 2014-06-06 | A kind of method improving Herba Fimbristylis dichotomae percentage of seedgermination |
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| CN201410247321.1A CN104054427B (en) | 2014-06-06 | 2014-06-06 | A kind of method improving Herba Fimbristylis dichotomae percentage of seedgermination |
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| CN104054427A true CN104054427A (en) | 2014-09-24 |
| CN104054427B CN104054427B (en) | 2016-07-06 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557121A (en) * | 2015-12-24 | 2016-05-11 | 中国热带农业科学院热带作物品种资源研究所 | Mariscus umbellatus seed depth dormancy breaking method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4624694A (en) * | 1983-08-15 | 1986-11-25 | Westvaco Corporation | Seed treatment method with aqueous suspension of alkali lignin |
| US4752319A (en) * | 1983-08-15 | 1988-06-21 | Westvaco Corporation | Seed treatment method with aqueous suspension of alkali lignin |
| CN102845158A (en) * | 2012-08-27 | 2013-01-02 | 中国热带农业科学院热带作物品种资源研究所 | Method for rapid release of Callicarpa nudiflora seed dormancy |
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2014
- 2014-06-06 CN CN201410247321.1A patent/CN104054427B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4624694A (en) * | 1983-08-15 | 1986-11-25 | Westvaco Corporation | Seed treatment method with aqueous suspension of alkali lignin |
| US4752319A (en) * | 1983-08-15 | 1988-06-21 | Westvaco Corporation | Seed treatment method with aqueous suspension of alkali lignin |
| CN102845158A (en) * | 2012-08-27 | 2013-01-02 | 中国热带农业科学院热带作物品种资源研究所 | Method for rapid release of Callicarpa nudiflora seed dormancy |
Non-Patent Citations (2)
| Title |
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| 房丽宁 等: "打破苔草种子休眠方法的研究", 《草业科学》 * |
| 房丽宁 等: "苔草种子休眠和萌发特性的研究", 《草地学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557121A (en) * | 2015-12-24 | 2016-05-11 | 中国热带农业科学院热带作物品种资源研究所 | Mariscus umbellatus seed depth dormancy breaking method |
| CN105557121B (en) * | 2015-12-24 | 2018-06-29 | 中国热带农业科学院热带作物品种资源研究所 | A kind of method for breaking conferted sawgrass herb seed deep-sleep |
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| CN104054427B (en) | 2016-07-06 |
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