CN105230455B - Fang Shi begonia water planting propagation methods - Google Patents
Fang Shi begonia water planting propagation methods Download PDFInfo
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- CN105230455B CN105230455B CN201510652729.1A CN201510652729A CN105230455B CN 105230455 B CN105230455 B CN 105230455B CN 201510652729 A CN201510652729 A CN 201510652729A CN 105230455 B CN105230455 B CN 105230455B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The present invention discloses a kind of Fang Shi begonias water planting propagation method, and mature leaf disinfection, one layer of absorbent cotton is spread in sterile petri dish, repaves one layer of sterile qualitative filter paper thereon；Blade is lain against on sterile qualitative filter paper, absorbent cotton is soaked with culture of rootage liquid and filter paper carries out culture of rootage, until petiole director goes out 4~7 adventitious roots；Supplement adventitious bud inducing liquid wetting absorbent cotton and filter paper carries out culture of rootage, until 5~10 buds are sprouted at place of taking root, the 1/2 MS grown cultures liquid that supplement sterile water is prepared continues to cultivate, until the bud development sprouted grows leaflet；Blade is sterilized in shading shed, is planted in matrix, spraying increase air humidity, obtains begonia seedling into shading shed.The Fang Shi begonias bred using this method, survival rate 99~100%；Germination rate improves 40%, and survival rate improves 30%, and nearly 1 times of cycle time, has dissemination in begonia conservation and production.
Description
Technical field
The present invention relates to technical field of plant asexual propagation, and in particular to a kind of Fang Shi begonias water planting propagation method.
Background technology
Fang Shi begonias（Begonia fangii）Compound leaf, blade leathery, vein is red, has higher ornamental value.
It can use at present using distilled water or sterile water as nutrient solution, the absorbent cotton culture dish using cut-off blade or leaf with petiol as material
Interior water planting propagation method, at blade cuts or petiole is taken root slowly, and 20~25 days, radical was few, 2~3, elongated；Do not sent out after taking root
Bud, even if germination, germinating time length, needs 30~35 days, and bud number is few, and only 3~5, germination percentage is low, and only 30~40%；During transplanting,
Root stretches into absorbent cotton, be easy to cause and is completely cured, and using peat or garden mould as cultivation matrix, soil easy ponding or hardened, causes root
Rot, and adventitious root water absorbing capacity is weak, photosynthesis is not strong, survival rate 60~70%, breeding cycle length, 60~65 days.
Therefore, it is necessary to improve the propagation method of Fang Shi begonias.
The content of the invention
The problems such as germination percentage is low, survival rate is low, the breeding cycle is long, the present invention are bred to solve current Fang Shi begonias water planting
There is provided it is a kind of easily take root, easily germination, breeding cycle short Fang Shi begonia water planting propagation methods.
The present invention is realized by following technical proposal：A kind of Fang Shi begonias water planting propagation method, by following each step：
（1）The mature leaf of healthy growth is chosen, with the alcohol disinfecting that volumetric concentration is 70%, petiole is cut short, makes each leaf
Piece retains the petiolule or common petiole of 0.5~1.0cm of length；
（2）The absorbent cotton of one layer of 0.3~0.5cm thickness is spread in sterile petri dish, repaves one layer of sterile qualitative filter paper thereon；
By step（1）Gained blade is lain against on sterile qualitative filter paper, soaks absorbent cotton and filter paper with following culture of rootage liquid：Sterile water
+ 10~30mg/L vitamin B1+10~20mg/L root-inducing powders（ABT）Solution or 10~30mg/L vitamin B1+1mg/L NAA+
0.1~0.3mg/L KT, and make sterile qualitative filter paper surface not ponding；Temperature be 23~25 DEG C, intensity of illumination be 3000~
Culture of rootage, daily optical culture 10h, light culture 14h, and supplement once above-mentioned life in every 2~3 days are carried out under conditions of 5000lux
Root nutrient solution, within control nutrient solution hydrops 1mL, keeps absorbent cotton and filter paper moistening, until petiole director go out 4~7 it is indefinite
Root；10~11 days after nutrient solution addition, and adventitious root is sturdy；
（3）After adventitious root is grown, following adventitious bud inducing liquid wetting absorbent cotton and filter paper are supplemented：Sterile water+10~20mg/
L vitamin B compound+2.0mg/L 6-BA, under conditions of temperature is 23~25 DEG C, intensity of illumination is 3000~5000lux into
Once above-mentioned adventitious bud inducing liquid, control are trained for row culture of rootage, daily optical culture 10h, light culture 14h, and every 2~3 days supplements
Within nutrient solution hydrops 1mL, absorbent cotton and filter paper moistening are kept, until 5~10 buds are sprouted at place of taking root；Adventitious bud inducing liquid adds
12~14 days afterwards, 5~10 buds are sprouted, and germination percentage is up to 70~80%；
（4）After germination, the 1/2 MS grown cultures liquid prepared with aforesaid operations supplement sterile water continues culture 8~10 days,
Until the bud development sprouted grows leaflet；
（5）In shading shed, with 800~1000 times of liquid of Bravo wettable powder of content 75%, above-mentioned blade is sterilized
3~5min, clear water are rinsed one time, planted in following matrix：Thick fertile soil:Garden mould or peat:Perlite=2:1:1, it is fixed to irrigate
After root water, manage under the following conditions：More than 15 DEG C of temperature, covering transparent plastic film keep humidity, spray into shading shed
Increase air humidity, air humidity is maintained at 75~85%, and daytime sprays water once every 2h, and film is taken off after 3 days, reduces water spray
Number makes air humidity be maintained at 65~75% to four times a day, until spire is grown, up to begonia seedling.Survival rate 99~
100%。
The present invention possesses advantages below and effect compared with conventional method：
1st, culture of rootage liquid of the invention uses vitamin B1, root-inducing powder or plant growth regulator, there is hestening rooting
Effect, take root fast, 10~11 days, root was healthy and strong；Adventitious bud inducing liquid uses vitamin b complex solution+2.0mg/L 6-BA,
Be conducive to adventitious bud formation, adventitious bud number is more, germination rate 70~80%；1/2MS grown cultures liquid promotes plant strain growth healthy and strong.
2nd, water planting breeding is improved, one layer of absorbent cotton upper berth aseptic filter paper, avoids root and stretch into absorbent cotton and cause to be completely cured.
3rd, water planting breeding is improved, during transplanting, blade 75% Bravo wettable powder, 800~1000 times of liquid disinfectants, are cultivated
Matrix uses thick fertile soil：Garden mould（Or peat）：Perlite 2：1：1, not only water conservation breathe freely but also be rich in nutrition, to keep air wet
Degree, covers transparent plastic film, sprays a water, survival rate 99~100% every 2h daytime.
4th, the Fang Shi begonias bred using this method, germination rate improve 40%, and survival rate improves 30%, cycle time nearly 1
Times, there is dissemination in begonia conservation and production.
Embodiment
Below by embodiment, the present invention will be further described.
Embodiment 1
（1）On June 10th, 2015, chooses the mature leaf of healthy growth, with the alcohol disinfecting that volumetric concentration is 70%, cuts
Short petiole, the petiolule or common petiole for each blade is retained 0.5~1.0cm of length；
（2）The absorbent cotton of one layer of 0.3~0.5cm thickness is spread in sterile petri dish, repaves one layer of sterile qualitative filter paper thereon；
Blade is lain against on sterile qualitative filter paper, soaks absorbent cotton and filter paper with following culture of rootage liquid：Sterile water+10mg/L dimension lifes
Plain B1+10mg/L root-inducing powders（ABT）Solution, and make sterile qualitative filter paper surface not ponding；Temperature be 23 DEG C, intensity of illumination be
Culture of rootage, daily optical culture 10h, light culture 14h, and supplement once above-mentioned life in every 2~3 days are carried out under conditions of 3000lux
Root nutrient solution, controls within nutrient solution hydrops 1mL, keeps absorbent cotton and filter paper moistening, on June 21st, 2015, petiole director went out 4
~7 sturdy adventitious roots；
（3）June 22, supplements following adventitious bud inducing liquid wetting absorbent cotton and filter paper：The compound dimension lifes of sterile water+10mg/L
Plain B+2.0mg/L 6-BA, culture of rootage is carried out under conditions of temperature is 23 DEG C, intensity of illumination is 3000lux, is trained per the daylight
10h, light culture 14h are supported, and supplements within every 2~3 days once above-mentioned adventitious bud inducing liquid, within control nutrient solution hydrops 1mL, is kept
5~10 buds are sprouted at absorbent cotton and filter paper moistening, place of taking root July 4；Germination percentage is up to 78%；
（4）July 5, the 1/2 MS grown cultures liquid prepared with aforesaid operations supplement sterile water continue culture 10 days, July
The bud development sprouted for 16th grows leaflet；
（5）July 17, in shading shed, with 800 times of liquid of Bravo wettable powder of content 75%, disappears above-mentioned blade
3~5min of poison, clear water are rinsed one time, planted in following matrix：Thick fertile soil:Garden mould:Perlite=2:1:1, irrigate root water
Afterwards, manage under the following conditions：More than 15 DEG C of temperature, covering transparent plastic film keep humidity, increase of spraying into shading shed
Air humidity, makes air humidity be maintained at 75~85%, and daytime sprays water once every 2h, and film is taken off after 3 days, reduces water spray number
To four times a day, air humidity is set to be maintained at 65~75%, until spire is grown, up to begonia seedling.Survival rate 100%, breeding
36 days cycles.
Embodiment 2
（1）The mature leaf of healthy growth is chosen, with the alcohol disinfecting that volumetric concentration is 70%, petiole is cut short, makes each leaf
Piece retains the petiolule or common petiole of 0.5~1.0cm of length；
（2）The absorbent cotton of one layer of 0.3~0.5cm thickness is spread in sterile petri dish, repaves one layer of sterile qualitative filter paper thereon；
Blade is lain against on sterile qualitative filter paper, soaks absorbent cotton and filter paper with following culture of rootage liquid：30mg/L vitamin B1s+
1mg/L NAA+0.3mg/L KT, and make sterile qualitative filter paper surface not ponding；Temperature be 23 DEG C, intensity of illumination be
Culture of rootage, daily optical culture 10h, light culture 14h, and supplement once above-mentioned life in every 2~3 days are carried out under conditions of 5000lux
Root nutrient solution, within control nutrient solution hydrops 1mL, keeps absorbent cotton and filter paper moistening, petiole director go out 4~7 it is sturdy indefinite
Root；
（3）Supplement following adventitious bud inducing liquid wetting absorbent cotton and filter paper：Sterile water+15mg/L vitamin B compounds+
2.0mg/L 6-BA, culture of rootage, daily optical culture are carried out under conditions of temperature is 23 DEG C, intensity of illumination is 5000lux
10h, light culture 14h, and once above-mentioned adventitious bud inducing liquid is supplemented within every 2~3 days, within control nutrient solution hydrops 1mL, keep de-
5~10 buds are sprouted at fat cotton and filter paper moistening, place of taking root；12~14 days after the addition of adventitious bud inducing liquid, 5~10 buds are sprouted,
And germination percentage is up to 80%；
（4）The 1/2 MS grown cultures liquid prepared with aforesaid operations supplement sterile water continues culture 8~10 days, the bud of sprouting
Development grows leaflet；
（5）In shading shed, with 1000 times of liquid of Bravo wettable powder of content 75%, to above-mentioned blade disinfection 3~
5min, clear water are rinsed one time, planted in following matrix：Thick fertile soil:Garden mould:Perlite=2:1:1, after irrigating root water,
Managed under the conditions of following：More than 15 DEG C of temperature, covering transparent plastic film keep humidity, and into shading shed, spraying increase air is wet
Degree, makes air humidity be maintained at 75~85%, and daytime sprays water once every 2h, and film is taken off after 3 days, reduces water spray number to one day 4
It is secondary, air humidity is maintained at 65~75%, until spire is grown, up to begonia seedling.Survival rate 100%, breeding cycle 33
My god.
Embodiment 3
（1）On June 26th, 2015, chooses the mature leaf of healthy growth, with the alcohol disinfecting that volumetric concentration is 70%, cuts
Short petiole, the petiolule or common petiole for each blade is retained 0.5~1.0cm of length；
（2）The absorbent cotton of one layer of 0.3~0.5cm thickness is spread in sterile petri dish, repaves one layer of sterile qualitative filter paper thereon；
Blade is lain against on sterile qualitative filter paper, soaks absorbent cotton and filter paper with following culture of rootage liquid：Sterile water+30mg/L dimension lifes
Plain B1+20mg/L root-inducing powders（ABT）Solution, and make sterile qualitative filter paper surface not ponding；Temperature be 24 DEG C, intensity of illumination be
Culture of rootage, daily optical culture 10h, light culture 14h, and supplement once above-mentioned life in every 2~3 days are carried out under conditions of 4000lux
Root nutrient solution, within control nutrient solution hydrops 1mL, keeps absorbent cotton and filter paper moistening, on July 8th, 2015 petiole director go out 4~
7 sturdy adventitious roots；
（3）July 9, supplements following adventitious bud inducing liquid wetting absorbent cotton and filter paper：The compound dimension lifes of sterile water+20mg/L
Plain B+2.0mg/L 6-BA, culture of rootage is carried out under conditions of temperature is 24 DEG C, intensity of illumination is 4000lux, is trained per the daylight
10h, light culture 14h are supported, and supplements within every 2~3 days once above-mentioned adventitious bud inducing liquid, within control nutrient solution hydrops 1mL, is kept
5~10 buds are sprouted at absorbent cotton and filter paper moistening, place of taking root July 23；Germination percentage is up to 80%；
（4）July 24, the 1/2 MS grown cultures liquid prepared with aforesaid operations supplement sterile water continue culture 9 days, August 2
The bud development that day sprouts grows leaflet；
（5）August 3 days, in shading shed, with 900 times of liquid of Bravo wettable powder of content 75%, disappears above-mentioned blade
3~5min of poison, clear water are rinsed one time, planted in following matrix：Thick fertile soil:Peat:Perlite=2:1:1, irrigate root water
Afterwards, manage under the following conditions：More than 15 DEG C of temperature, covering transparent plastic film keep humidity, increase of spraying into shading shed
Air humidity, makes air humidity be maintained at 75~85%, and daytime sprays water once every 2h, and film is taken off after 3 days, reduces water spray number
To four times a day, air humidity is set to be maintained at 65~75%, until spire is grown, up to begonia seedling.Survival rate 100%, breeding
38 days cycles.
Embodiment 4
（1 chooses the mature leaf of healthy growth, with the alcohol disinfecting that volumetric concentration is 70%, cuts short petiole, makes each leaf
Piece retains the petiolule or common petiole of 0.5~1.0cm of length；
（2）The absorbent cotton of one layer of 0.3~0.5cm thickness is spread in sterile petri dish, repaves one layer of sterile qualitative filter paper thereon；
Blade is lain against on sterile qualitative filter paper, soaks absorbent cotton and filter paper with following culture of rootage liquid：10mg/L vitamin B1s+
1mg/L NAA+0.1mg/L KT, and make sterile qualitative filter paper surface not ponding；Temperature be 25 DEG C, intensity of illumination be
Culture of rootage, daily optical culture 10h, light culture 14h, and supplement once above-mentioned life in every 2~3 days are carried out under conditions of 5000lux
Root nutrient solution, within control nutrient solution hydrops 1mL, keeps absorbent cotton and filter paper moistening, petiole director go out 4~7 it is sturdy indefinite
Root；
（3）Supplement following adventitious bud inducing liquid wetting absorbent cotton and filter paper：Sterile water+20mg/L vitamin B compounds+
2.0mg/L 6-BA, culture of rootage is carried out under conditions of temperature is 25 DEG C, intensity of illumination is 3000~5000lux, per the daylight
10h, light culture 14h are cultivated, and supplements within every 2~3 days once above-mentioned adventitious bud inducing liquid, within control nutrient solution hydrops 1mL, is protected
Absorbent cotton and filter paper moistening are held, 5~10 buds are sprouted at place of taking root；12~14 days after the addition of adventitious bud inducing liquid, 5~10 are sprouted
Bud, and germination percentage is up to 77%；
（4）The 1/2 MS grown cultures liquid prepared with aforesaid operations supplement sterile water continues culture 8 days, the bud development of sprouting
Grow leaflet；
（5）In shading shed, with 1000 times of liquid of Bravo wettable powder of content 75%, to above-mentioned blade disinfection 3~
5min, clear water are rinsed one time, planted in following matrix：Thick fertile soil:Peat:Perlite=2:1:1, after irrigating root water,
Managed under the conditions of following：More than 15 DEG C of temperature, covering transparent plastic film keep humidity, and into shading shed, spraying increase air is wet
Degree, makes air humidity be maintained at 75~85%, and daytime sprays water once every 2h, and film is taken off after 3 days, reduces water spray number to one day 4
It is secondary, air humidity is maintained at 65~75%, until spire is grown, up to begonia seedling.Survival rate 100%, breeding cycle 32
My god.
Claims (1)
1. a kind of Fang Shi begonias water planting propagation method, it is characterised in that pass through following each step：
（1）The mature leaf of healthy growth is chosen, with the alcohol disinfecting that volumetric concentration is 70%, petiole is cut short, protects each blade
Stay the petiolule or common petiole of 0.5~1.0cm of length；
（2）The absorbent cotton of one layer of 0.3~0.5cm thickness is spread in sterile petri dish, repaves one layer of sterile qualitative filter paper thereon；Will step
Suddenly（1）Gained blade is lain against on sterile qualitative filter paper, soaks absorbent cotton and filter paper with following culture of rootage liquid：Sterile water+10
~30mg/L+10~20mg/L of vitamin B1 rooting powder solutions or 10~30mg/L vitamin B1+1mg/L NAA+0.1~
0.3mg/L KT；Culture of rootage is carried out under conditions of temperature is 23~25 DEG C, intensity of illumination is 3000~5000lux, daily
Optical culture 10h, light culture 14h, and once above-mentioned culture of rootage liquid, holding absorbent cotton and filter paper moisten every 2~3 days supplements, directly
To petiole, director goes out adventitious root；
（3）After adventitious root is grown, following adventitious bud inducing liquid wetting absorbent cotton and filter paper are supplemented：Sterile water+10~20mg/L is multiple
Vitamin B+2.0mg/L 6-BA are closed, are carried out under conditions of temperature is 23~25 DEG C, intensity of illumination is 3000~5000lux
Culture of rootage, daily optical culture 10h, light culture 14h, and every 2~3 days supplement once above-mentioned adventitious bud inducing liquid, keep degreasing
Cotton and filter paper moistening, until 5~10 buds are sprouted at place of taking root；
（4）After germination, the 1/2 MS grown cultures liquid that supplement sterile water is prepared continues culture 8~10 days, until the bud hair sprouted
Educate and grow leaflet；
（5）In shading shed, with 800~1000 times of liquid of Bravo wettable powder of content 75%, to step（4）Leaflet disappear
3~5min of poison, clear water are rinsed one time, planted in following matrix：Thick fertile soil:Garden mould or peat:Perlite=2:1:1, irrigate
After root water, manage under the following conditions：More than 15 DEG C of temperature, covering transparent plastic film keep humidity, are sprayed into shading shed
Mist increases air humidity, air humidity is maintained at 75~85%, and daytime sprays water once every 2h, and film is taken off after 3 days, reduces spray
Waterside number makes air humidity be maintained at 65~75% to four times a day, until spire is grown, up to begonia seedling.
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105815196B (en) * | 2016-03-29 | 2019-06-28 | 河南省农业科学院经济作物研究所 | Utilize the method for the quick child care breeding sesame of sesame blade |
| CN105746322B (en) * | 2016-03-29 | 2019-06-28 | 河南省农业科学院经济作物研究所 | Utilize the method for the quick child care breeding cotton of cotton leaf |
| CN106134956A (en) * | 2016-06-29 | 2016-11-23 | 百色学院 | A kind of foliage plant black beauty ciltivating process |
| CN107306770A (en) * | 2017-06-27 | 2017-11-03 | 福建百卉花艺有限公司 | A kind of ciltivating process of money tree |
| CN107535351B (en) * | 2017-09-26 | 2019-08-27 | 中国科学院昆明植物研究所 | The cultivation of ' three split ' begonia and cultural method |
| CN108901800A (en) * | 2018-08-30 | 2018-11-30 | 常熟市魔方园艺科技有限公司 | A kind of water planting root system induction method of Rieger Begonia |
| CN112400584A (en) * | 2019-08-19 | 2021-02-26 | 陈芳淼 | Asexual rapid propagation method for dicotyledons |
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