CN111183887A - Rapid cutting propagation method for zinc-cadmium super-accumulation plant Sedum plumbizincicola - Google Patents
Rapid cutting propagation method for zinc-cadmium super-accumulation plant Sedum plumbizincicola Download PDFInfo
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- CN111183887A CN111183887A CN201811355814.1A CN201811355814A CN111183887A CN 111183887 A CN111183887 A CN 111183887A CN 201811355814 A CN201811355814 A CN 201811355814A CN 111183887 A CN111183887 A CN 111183887A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
Abstract
A rapid cutting propagation method for repairing plant Sedum plumbizincicola comprises the following steps: intercepting the stem segment of the Sedum plumbizincicola, inserting the base into a rooting culture solution, culturing for 7-20 days at 15-23 ℃, transplanting into a seedling culture medium, managing according to a traditional farming method, harvesting the overground part after the plant grows to 15-30 cm, cutting the overground part as a seedling into stem segments with axillary buds of 6-10cm, and cutting the stem segments into the seedling culture medium or a nutrient solution; the rooting culture solution is a basic nutrient solution added with auxin analogs and cytokinin analogs in a concentration ratio of 1:2, the basic nutrient solution is 50% of Japanese gardening formula nutrient solution, and the seedling culture substrate is vermiculite: perlite or organic soil substrate in a ratio of 3: 1. The method for cutting propagation of sedum plumbizincicola greatly improves the cutting propagation rate, the rooting speed and the survival rate of sedum plumbizincicola, solves the key technical problem of rapid propagation of sedum plumbizincicola, and has important value for rapid propagation of sedum plumbizincicola and industrialized seedling production and popularization.
Description
Technical Field
The invention belongs to the technical field of rapid propagation of heavy metal hyperaccumulator plants, and particularly relates to a rapid cutting propagation method of zinc-cadmium hyperaccumulator plants Sedum plumbizincicola.
Background
The Sedum plumbizincicola is perennial fleshy herb; the rhizome is long, transverse: root and beard shape. The sterile branches are upright, densely clustered and multi-branched; usually 1-3 scapes grow from sterile branch clusters, usually slightly higher than the sterile branches, the height can reach more than 35 cm, and the thickness can reach 0.8 cm. The leaves grow alternately, the lower leaves of the stem usually fall early, the top leaves are dense, the shape of an oval or an inverted oval is long and long, the length is l-5 cm, the width is 0.5-1.5 cm, the tip is blunt and sharp, the base is wedge-shaped, the stem gradually narrows to form a long false stem, and the base on the back has an unobvious short moment. The inflorescence grows from the top of the stem and is a poly-umbrella inflorescence with 2-3 branches (sometimes 4 branches). Flower without handle, narrow triangle, 1-2 mm, blunt tip: 4, yellow petals, which are needle-shaped, have the length of 4-6 mm and the width of 1-1.5 mm; the stamen 8 and 2 wheels are slightly shorter than the petals, and the base of the inner wheel is connected with the petals. Blind fluorescent fruit has many seeds; the seeds are oblong, wrinkled, dark brownish-brown, about 1 mm long, and have a finger-like protrusion formed by the remaining bead stalk at one end. The flowering period is 6-7 months, and the fruit period is 8 months.
The treatment of the problem that farmland soil in China is polluted by heavy metal is increasingly emphasized. The physical and chemical remediation technology (including isolation embedding technology, solidification stabilizing technology, chemical stabilizing technology, electric remediation technology and the like) is adopted to treat heavy metal soil pollution, so that the aim of removing pollution is not achieved fundamentally, and secondary pollution of soil can be caused by the use of chemical agents. Phytoremediation is a new environmental remediation technology for treating soil pollution by using heavy metal super-accumulation or resistant plants, and has the advantages of low cost, no secondary pollution, land resource saving, environmental friendliness, sustainability and the like. The key point of phytoremediation is to search or cultivate specific germplasm resources which can tolerate and super accumulate one or more heavy metals, have large biomass, wide adaptability and rapid propagation. The Sedum plumbizincicola is a newly discovered heavy metal tolerant and hyper-enriched plant in China, is favored to be grown in Pb and Zn-rich ore areas, and has the capacity of super-accumulating zinc and cadmium. The growth habit of the sedum aizoon is basically similar to that of other sedum aizoon, the sedum aizoon naturally distributes in places such as Zhejiang, and can grow normally in most of China, and the sedum aizoon has wide adaptability, large biomass and high growth speed. Therefore, the method has the advantages of low restoration cost, small disturbance to the environment, no secondary pollution, strong sustainability and the like when being applied to restoration of heavy metal polluted soil, and has the function of beautifying the environment, thereby having wide prospect.
In view of the wide application prospect of the Sedum plumbizincicola, the seedling demand of the Sedum plumbizincicola is gradually increased. The seeds of the Sedum plumbizincicola are tiny, the sowing and seedling raising technology is complicated, the period is long, the seedling is slow, the cutting propagation technology is usually adopted in production, and direct cutting is usually slow in rooting and easy to infect and attack, so that the growth and the propagation are slow, and the large-scale popularization and planting of the Sedum plumbizincite is influenced. Therefore, how to solve the difficult problems of difficult rooting and slow propagation of the sedum plumbizincicola is the key of the rapid propagation of the sedum plumbizincicola and has important value for the industrialized seedling production and the large-scale popularization and application of the sedum plumbizincicola.
Disclosure of Invention
The invention aims to provide a rapid cutting propagation method of a zinc-cadmium hyperaccumulation plant Sedum plumbizincicola, aiming at solving some technical problems mentioned in the background.
The invention provides a rapid cutting propagation method of Sedum plumbizincicola, which comprises the following steps: intercepting stem segment of disease-free and insect-free Sedum plumbizincicola, inserting the base of the stem segment into rooting culture solution, and culturing at 15-23 deg.C for 7-20 days; during the period, the culture solution is replaced every 5-10 days, after the root system grows out, the plant is transplanted into a seedling culture medium, the growth is managed according to the traditional farming method, the overground part is harvested when the plant grows to 15-30 cm, the plant is used as a seedling and is cut into stem segments with axillary buds of more than 6cm, the stem segments are inserted into the seedling culture medium or nutrient solution, and the growth is managed according to the traditional farming method, so that the seedling is obtained.
The rooting culture solution is a basic nutrient solution added with a plant growth regulator, the plant growth regulator comprises auxin analogue sodium naphthalene acetate and cytokinin analogue potassium indolebutyrate with a concentration ratio of 1:2, and the basic nutrient solution is 50% of a Japanese gardening formula balanced nutrient solution.
The balanced nutrient solution for the Japanese gardening formula comprises 945mg/L calcium nitrate tetrahydrate, 809mg/L potassium chloride, 153mg/L ammonium dihydrogen phosphate, 493mg/L magnesium sulfate heptahydrate, 3mg/L boric acid, 2mg/L manganese sulfate monohydrate, 0.22mg/L zinc sulfate heptahydrate, 0.05mg/L copper sulfate pentahydrate, 0.02mg/L ammonium molybdate heptahydrate and 20mg/L sodium iron ethylenediaminetetraacetate, and the nutrient solution is diluted by 50% after being prepared.
The plant growth regulator preferably comprises 0.2mg/L of sodium naphthalene acetate and 0.4mg/L of potassium indolebutyrate.
The length of the stem section is preferably 6-10cm, the upper end of the stem section is flattened, and the base of the stem section is cut into an inclined plane.
The length of the stem section inserted into the rooting culture solution is preferably 2-3 cm.
As is conventional in the art, the rooting medium is typically contained in a glass or plastic container and sterilized for use, preferably, the rooting medium is contained in a glass or plastic container and sterilized for culturing the stem segments, and the glass or plastic container is covered with a light-shielding material at a position below the container mouth to ensure that only the part of the plant stem segment outside the container is visible and the rest is invisible after the stem segment is inserted for culturing.
The Sedum plumbizincicola seedling culture medium is a vermiculite-perlite matrix or an organic matter matrix with the ratio of 3: 1.
The sedum plumbizincicola used in the technical scheme of the invention has stronger enrichment capacity on heavy metal zinc and cadmium in soil, the sedum plumbizincicola is used for repairing cadmium-polluted farmland soil and has the characteristics of low investment cost, extensive management, prevention of soil exposure in winter and the like, and bright yellow clusters are formed in summer, so that the florescence is longer, and the sedum plumbizincicola has better ornamental value and environmental benefit, and therefore, the sedum plumbizincicola has wide repair potential and popularization and application prospects. The method for cultivating the sedum plumbizincicola by combining water culture cutting rooting with seedling culture medium provided by the invention obviously improves the cutting propagation rate, rooting speed and survival rate of the sedum plumbizincicola, solves the key technical problem of rapid propagation of the sedum plumbizincicola and has important value for rapid propagation of the sedum plumbizincicola and industrial seedling culture production and popularization.
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FIG. 1 shows a flow chart of a rapid water culture cutting propagation method of Sedum plumbizincicola according to the present invention.
Detailed Description
To more clearly illustrate the objects, technical solutions and advantages of the present invention, the present invention is further described in detail with reference to specific examples below.
Example 1, using the indoor hydroponic cutting test method, the base nutrient solution was a 50% balanced nutrient solution of the japanese garden formula, including 945mg/L calcium nitrate tetrahydrate, 809mg/L potassium chloride, 153mg/L ammonium dihydrogen phosphate, 493mg/L magnesium sulfate heptahydrate, 3mg/L boric acid, 2mg/L manganese sulfate monohydrate, 0.22mg/L zinc sulfate heptahydrate, 0.05mg/L copper sulfate pentahydrate, 0.02mg/L ammonium molybdate heptahydrate, and 20mg/L ferric ethylenediaminetetraacetate sodium, all at 1/2 doses. Plant growth regulators of sodium naphthalene acetate and potassium indolebutyrate were added to the base nutrient solution to concentrations of 0.2mg/L and 0.4mg/L, respectively.
Specifically, first, a basic nutrient solution is prepared: respectively preparing 100 times of concentrated mother liquor I (94.5 g of calcium nitrate tetrahydrate and 80.9g of potassium chloride are respectively weighed, the water volume is 1000mL), 100 times of concentrated mother liquor II (15.3 g of ammonium dihydrogen phosphate and 49.3g of magnesium sulfate heptahydrate are respectively weighed and dissolved in 1000mL of water), 1000 times of concentrated mother liquor III (3 g of boric acid and 0.02g of ammonium molybdate heptahydrate are respectively weighed and dissolved in 1000mL of water), 1000 times of concentrated mother liquor IV (2 g of manganese sulfate monohydrate, 0.22g of zinc sulfate heptahydrate and 0.05g of copper sulfate pentahydrate are respectively weighed and dissolved in 1000mL of water), and 1000 times of concentrated mother liquor V (20g of sodium ferric ethylenediamine tetraacetate is dissolved in 1000mL of water).
Then, preparing a plant growth regulator mother solution: for sodium naphthylacetate, 0.1g of sodium naphthylacetate is dissolved in ultrapure water, and the volume is determined to 1L to obtain 0.1g/L of mother liquor; for potassium indolebutyrate, 0.0842g of indolebutyric acid and 0.0232g of potassium hydroxide are dissolved in water, and the volume is determined to be 1L after the indole butyric acid and the potassium indolebutyrate are fully dissolved, so that 0.1g/L of mother liquor is obtained.
Then, preparing a rooting culture solution by using each mother solution of the basic nutrient solution and a mother solution of a plant growth regulator: respectively taking 5mL, 0.5mL and 0.5mL of the mother liquor from the basic nutrient solution A to the mother liquor E, then taking a plurality of plant growth regulator sodium naphthylacetate mother liquor and indole butyric acid potassium mother liquor (the final concentrations are respectively 0.2mg/L and 0.4mg/L), fully and uniformly mixing all the components, and diluting to 1L for later use. According to the same method, the dosage of one to five mother liquor is not changed, and the final concentrations of sodium naphthalene acetate and potassium indolebutyrate are respectively as follows: 0mg/L and 0mg/L, 0.2mg/L and 0.2mg/L, 0.4mg/L and the like of working culture solution with different plant growth regulator concentrations), selecting a transparent plastic basin with the capacity of 1L of 18 multiplied by 12cm as a culture container, wrapping tin foil paper outside the container to shade light and avoid green algae breeding, soaking and disinfecting for 10-15min by using 0.5% potassium permanganate solution, and washing with clear water for later use. And (3) taking a polyethylene foam board with the thickness of 1cm as a cutting board for the rapid water culture cutting rooting of the sedum plumbizincicola, cutting the sedum plumbizincicola into a cutting board slightly smaller than a plastic pot, and punching 40 holes with the thickness of about 0.5cm on each cutting board for cutting branches.
Selecting annual strong and disease and insect pest-free Sedum plumbizincicola, cutting stem into 6cm length, cutting the upper end of stem into flat, cutting the lower end into inclined plane, and cutting more than 200 branches for use.
2/3 volumes of rooting culture solution (about 600mL) is added into the culture container, 40 rhodiola rosea branches are inserted into each cutting plate, cutting slips are inserted into holes of a foam plate 1/3 and are fixed by sponge, and the foam plate is placed on a bottle or a pot, so that stem segments 2-3cm from the base of the cutting slips can contact with the rooting culture solution. Then culturing at 15-23 deg.C, and continuously observing rooting condition of Sedum plumbizincicola. During the period, the culture medium was replaced every 5 to 10 days.
The result shows that the rhodiola crenulata hydroponic root is sensitive to the proportion and the concentration of the plant growth regulator, and the test result can be obviously influenced by slight adjustment. In the example, the sodium naphthalene acetate and the potassium indolebutyrate are cultured under the conditions that the concentrations are 0.2mg/L and 0.4mg/L respectively, the effect is optimal, the stem section of the Sedum plumbizincicola starts to root in about one week, and the root quantity is very rich after 20 days of culture.
Subsequently, the sedum plumbizincicola with good root systems is transferred to a vermiculite: perlite seedling culture medium with a ratio of 3: 1, the sedum plumbizincicola is managed according to a traditional farming method, during the period, a small amount of organic fertilizer is applied every 15 days, and the transferred cuttings grow healthily and rapidly on the seedling culture medium. After culturing for 40-50 days, the Sedum plumbizincicola grows to 15-30 cm, the overground part is harvested, the Sedum plumbizincicola is used as a seedling, the seedling is cut into more than 6cm stem segments with axillary buds, the stem segments are inserted into a seedling culture medium or nutrient solution, and the growth is managed according to the traditional farming method. By the method, a large amount of rhodiola rosea rooting seedlings can be obtained in a short time.
Example 2, indoor hydroponic cuttage experiments were conducted using the method of example 1 to prepare 50% balanced nutrient solution for Japanese horticulture formulations with plant growth regulators sodium naphthaleneacetate and potassium indolebutyrate at concentrations of 0.2mg/L and 0.4mg/L, respectively.
Selecting a transparent plastic basin with a capacity of 2L and a length of 36 multiplied by 24cm as a culture container, shading the container with black cloth, disinfecting the container with 0.3 percent of 84 disinfectant for 20 to 30min, and washing the container with clear water for later use. A polyethylene foam board with the thickness of 1.5cm is used as a cuttage board, the cuttage board is cut into a size slightly smaller than that of the plastic basin, and 100 small holes of about 0.2cm are punched on each cuttage board for cuttage branches.
Selecting annual strong and disease and insect pest-free Sedum plumbizincicola, cutting stem into 9cm length, cutting the upper end of the stem into flat, cutting the lower end into inclined plane, and cutting more than 400 branches for standby.
The culture container holds 2/3 volumes of rooting culture solution (about 1200mL), each cutting plate is inserted with 100 rhodiola rosea branches, the cutting slips are inserted into the holes of the foam plate, the foam plate is placed in the basin and slightly supported by a wooden frame, the air permeability of the foam plate is kept, and the stem section of 2-3cm of the base of the cutting slips is contacted with the rooting culture solution. Then culturing at room temperature, and observing the rooting condition of the Sedum plumbizincicola. During the period, the culture medium was replaced every 5 to 10 days.
The stem segment of the Sedum plumbizincicola starts to root in about one week, and the root amount is very rich after 20 days of culture. And then, the sedum plumbizincicola with good root systems is transferred to nutrient soil sprayed with 0.05 percent potassium permanganate in advance for cultivation, and is managed according to a traditional method, and a small amount of compound fertilizer is applied. Culturing for 40-50 days, harvesting aerial parts after the Sedum plumbizincicola grows to about 20cm, cutting into 9cm above stem segments with axillary buds, cutting into nutrient solution, performing rooting culture, and transferring the rooted cuttings into pre-sterilized nutrient soil after 20 days for cultivation. The method can make a small amount of cuttings left after withering during substrate cultivation regenerate in water, and can also obtain a large amount of rhodiola rosea rooting seedlings.
The foregoing is a description of embodiments of the invention only, and is not intended to be limiting, since any modifications or equivalents may be resorted to, falling within the scope of the invention.
Claims (7)
1. A rapid cutting propagation method for a zinc-cadmium hyper-accumulation plant Sedum plumbizincicola mainly comprises two stages of water culture cutting rooting culture and seedling culture medium culture of Sedum plumbizincicola stem segments, and is characterized by mainly comprising the following steps: intercepting disease and pest free Sedum plumbizincicola stems with over 6cm axillary buds, inserting the base parts of the stems into a rooting culture solution, and culturing for 7-20 days at 15-23 ℃; during the period, the culture solution is replaced every 5-10 days, after the root system grows, the plant is transplanted into a seedling substrate, the growth is managed according to the traditional farming method, when the plant grows to 15-30 cm and the overground part is harvested, the plant is cut into 6-10cm stem segments with axillary buds as seedlings, the stem segments are inserted into the seedling substrate or nutrient solution, the growth is managed according to the traditional farming method, and the seedlings which can be planted outdoors are obtained;
the rooting culture solution is a basic nutrient solution added with a plant growth regulator, the plant growth regulator comprises auxin analogue sodium naphthalene acetate and cytokinin analogue potassium indolebutyrate with a concentration ratio of 1:2, and the basic nutrient solution is 50% of a Japanese gardening formula balanced nutrient solution.
2. The method for water culture cutting rooting of Sedum plumbizincicola according to claim 1, wherein the balanced nutrient solution in the Japanese gardening formula comprises 945mg/L calcium nitrate tetrahydrate, 809mg/L potassium chloride, 153mg/L ammonium dihydrogen phosphate, 493mg/L magnesium sulfate heptahydrate, 3mg/L boric acid, 2mg/L manganese sulfate monohydrate, 0.22mg/L zinc sulfate heptahydrate, 0.05mg/L copper sulfate pentahydrate, 0.02mg/L ammonium molybdate heptahydrate and 20mg/L sodium iron ethylenediaminetetraacetate, and the nutrient solution is diluted by 50% after being prepared.
3. The method for hydroponically cutting and rooting culture of the Sedum plumbizincicola according to claim 1, wherein the plant growth regulator comprises 0.2mg/L of sodium naphthaleneacetate and 0.4mg/L of potassium indolebutyrate.
4. The method for the hydroponic cutting rooting culture of the Sedum plumbizincicola according to claim 1, wherein the length of the stem section is 6-10cm, the upper end of the stem section is cut flat, and the base of the stem section is cut into a slope.
5. The method for water culture, cutting and rooting culture of the Sedum plumbizincicola according to claim 1, wherein the length of the stem segment inserted into the rooting culture solution is 2-3 cm.
6. The method for the hydroponic cutting rooting culture of the rhodiola rosea according to claim 1, wherein the rooting culture solution is contained in a glass or plastic container and is used for culturing the stem segments after being sterilized, and the part below the container opening of the glass or plastic container is wrapped by a shading material.
7. The rhodiola crenulata seedling culture medium according to claim 1 is a vermiculite-perlite medium or an organic soil medium in a ratio of 3: 1.
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| CN201811355814.1A CN111183887A (en) | 2018-11-14 | 2018-11-14 | Rapid cutting propagation method for zinc-cadmium super-accumulation plant Sedum plumbizincicola |
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| CN201811355814.1A CN111183887A (en) | 2018-11-14 | 2018-11-14 | Rapid cutting propagation method for zinc-cadmium super-accumulation plant Sedum plumbizincicola |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113336577A (en) * | 2021-04-19 | 2021-09-03 | 生态环境部华南环境科学研究所 | Preparation and application of plant source special water fertilizer for sedum plumbizincicola |
| CN115997566A (en) * | 2022-12-22 | 2023-04-25 | 延边大学 | Rapid cutting propagation method for rhodiola sachalinensis |
-
2018
- 2018-11-14 CN CN201811355814.1A patent/CN111183887A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113336577A (en) * | 2021-04-19 | 2021-09-03 | 生态环境部华南环境科学研究所 | Preparation and application of plant source special water fertilizer for sedum plumbizincicola |
| CN115997566A (en) * | 2022-12-22 | 2023-04-25 | 延边大学 | Rapid cutting propagation method for rhodiola sachalinensis |
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