CN106489503A - Oil tree peony Clonal regeneration method for culturing seedlings - Google Patents
Oil tree peony Clonal regeneration method for culturing seedlings Download PDFInfo
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- CN106489503A CN106489503A CN201610983359.4A CN201610983359A CN106489503A CN 106489503 A CN106489503 A CN 106489503A CN 201610983359 A CN201610983359 A CN 201610983359A CN 106489503 A CN106489503 A CN 106489503A
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- tree peony
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The present invention relates to a kind of oil tree peony Clonal regeneration method for culturing seedlings, belongs to oil tree peony new varieties nursery stock fast breeding field.The method comprising the steps of：(1) bulbil explant is selected；(2) break bulbil explant dormancy；(3) seedling tissue cultures；(4) addressing in nursery；(5) construction of planting greenhouse；(6) seedbed is built；(7) tissue culture transplantation of seedlings nursery；(8) seedling is cultivated after tissue culture transplantation of seedlings.The method of the invention can realize the asexual quick breeding of tree peony nursery stock, and tree peony seedling propagation coefficient is significantly increased, and shorten seedling cultivation period, effectively alleviate market supply pressure of the current oil with tree peony new varieties nursery stock, promote oil tree peony new varieties to update；This method improves 35 times than tree peony nursery stock tradition mating system, breeding coefficient, and growing-seedling period shortens 20%, improves more than 15% into shoot survival percent.
Description
Technical field
The present invention relates to a kind of oil tree peony Clonal regeneration method for culturing seedlings, belongs to oil tree peony new varieties nursery stock fast breeding
Field.
Background technology
With economic development and the raising of scientific and technological level, the series research and development of peony product are substantially improved with deep processing level,
Tree peony industry by the past single view and admire expansion to multiple fields such as edible, medicinal, health cares, particularly oily sight is had concurrently with which with tree peony
Reward is worth the unique advantage with economic worth, shows huge potentiality to be exploited and vast potential for future development.Tree peony industry into
For our province growth of agricultural efficiency, the specialty industries of increasing peasant income and important industry.The beginning of this year, general office of provincial government issue《With regard to printing and distributing
The notice of Shandong Province's tree peony industrial development planning (2015-2020)》(Lu Zheng does word (2015) 7), by Huanghe delta ground
The northwest in Shandong Province Yellow River floodplain Industrial Area of tree peony estate planning is listed in area in, is that the whole province tree peony gives priority to region, with oil with tree peony is
Main, take into account view and admire with medicinal.《Planning》Regulation：Strive that each counties and districts sets up 1 more than 500 mu of demonstration field.Use for meeting oil
The ever-increasing market demand of tree peony new varieties nursery stock, for traditional tree peony seedling-wood breeding method (seed growing) breeding cycle
Long, breed the low problem of coefficient, using tissue cultures can a large amount of breed good strains in a short time advantage, test sufficiently
On the basis of research, summarize and define " oil tree peony Clonal regeneration method for culturing seedlings ".
Content of the invention
According to deficiency of the prior art above, it is an object of the invention to provide a kind of oil tree peony Clonal regeneration nursery side
Method, can realize the asexual quick breeding of tree peony nursery stock, and tree peony seedling propagation coefficient is significantly increased, and shorten seedling cultivation period, have
Effect alleviates market supply pressure of the current oil with tree peony new varieties nursery stock, promotes oil tree peony new varieties to update.
Oil tree peony Clonal regeneration method for culturing seedlings of the present invention, comprises the steps：
(1) bulbil explant is selected：It is explant to select cane bottom bulbil；
(2) break bulbil explant dormancy：First bulbil is sterilized, is then soaked using biological bactericide；
(3) seedling tissue cultures：In culturing room, proliferated culture medium, root induction culture medium, root growth culture is adopted successively
Base is cultivated to the bulbil after step (2) process, obtains plantlet in vitro；
(4) addressing in nursery：Nursery selects open level land on the sunny side, it is desirable to traffic convenience, has abundant water resources, totally, power supply
Convenient, away from epidemic-stricken area；
(5) construction of planting greenhouse：Planting greenhouse be flat roof type booth, frame be provided with degree of shading be 70-80% screening
Shady net, hydropower installation are used in place afterwards；
(6) seedbed is built：Bed surface requires smooth, bedside 1-1.2m, seedbed spacing 50-60cm；The fertilizer for becoming thoroughly decomposed is filled
Enter the nutrition cup that specification is 12cm × 10cm to be placed in seedbed, at least 7d before transplanting is poured with 500-800 times of carbendazim solution
Wet, open film after 3-5d in film cover, after irrigating by seedling；
(7) tissue culture transplantation of seedlings nursery：
Plantlet in vitro is transferred in planting greenhouse from culturing room, 7-10d hardenings are placed, then, with clear water by the training of root
Foster base is rinsed well, then with planting in nutrition cup after the mixed solution immersion of 800-1000 times of carbendazim and root-inducing powder, with punching
With kind；
(8) seedling is cultivated after tissue culture transplantation of seedlings：
Plantlet in vitro is planted to after nutrition cup, and then seedbed plastic covering film 7-10d takes off film, is taken off after film to planting greenhouse
Shelter from heat or light；Take off film at the same pour permeable, see later dry just pour, watering every time irrigates, and plantlet in vitro grows into 5-6 strains/clump, 10-
When 15cm is high, basin plant division is changed；Plantlet in vitro grows into long more than the 4cm of branch, and more than branch footpath 0.25cm, long more than the 10cm of root, root are thick
During more than 0.3cm, go out garden, listing.
In step (1), the next bottom for stalk of described stalk.
In step (1), the phase of drawing materials that bulbil explant is selected is the 3-4 months.
In step (2), sterilization be adopt mass concentration for 0.1% HgCl2Immersion 7-9min.
In step (2), soaked twice using biological bactericide, each soak time is 10-20min, and biological bactericide is
Mountain agriculture one.
In step (3), proliferated culture medium is：MS+6-BA1.0mg/L+NAA0.4-0.6mg/L+3-4mg/LAgNO3；Root
Inducing culture is：WPM+IBA6.0mg/L+ sucrose 20g/L；Root growth culture medium is：WPM+IBA 6.0mg/L+ sucrose 40g/
L.
In step (5), described planting greenhouse highly preferred for more than 2m, be easy to operate.
In step (5), the trend of planting greenhouse is east-west, so as to daylighting, insulation, windproof.Planting greenhouse requires flat
Whole, without weeds, long-term nursery can do skeleton with galvanized pipe, and interim canopy can do skeleton with bamboo and wood materials.
In step (6), the fertilizer loading specification that becomes thoroughly decomposed expires to about ninety percent for the nutrition cup of 12cm × 10cm, the battalion for installing
Foster cup vertically, is neatly put, and often capable cup number is consistent, is easy to statistical magnitude.
In step (7), in the mixed solution of carbendazim and root-inducing powder, carbendazim is 1 with the mass ratio of root-inducing powder:1.
In step (8), during the plastic covering film of seedbed, nocturnal temperature is kept for more than 15 DEG C, and the temperature on daytime is less than 30
℃.
In step (8), the prevention and control of plant diseases, pest control after tissue culture transplantation of seedlings, is carried out, method is as follows：Using 1000 times of carbendazim solutions or
The high-pressure fog of Mancozeb solution carries out the preventing and treating of disease；Using 1000 times of 20% Fenpropathrin, 1000 times of liquid and 48% chlopyrifos
Liquid is used alternatingly control aphid, mite class harm.
In step (8), apply fertilizer after tissue culture transplantation of seedlings, method is as follows：Plantlet in vitro starts to apply fertilizer after extracting 1-2 piece young leaves out, applies
Fertile species is urea or one or two the mixture in composite fertilizer, and the mass concentration of fertilising is 0.1%-0.3%, applies fertilizer dense
Spend and be incremented by with the growth of plantlet in vitro.
Preferably, early stage mainly applies urea, and concentration is 0.1%, and the later stage can properly increase 0.2%～0.3% concentration, match somebody with somebody
A small amount of composite fertilizer is applied in conjunction.
Planting greenhouse is sheltered from heat or light after taking off film, prevent sunshine from burning blade.
In the present invention, position and the time, break medicament and formula used by sprout dormancy that tree peony bulbil explant is selected, this
It is the key technology for realizing tissue cultures.
Tissue cultures, the selection, formula including culture medium are to realize oil tree peony nursery stock fast-propagation, shorten and cultivate week
The key technology of phase.
Nursery standard garden is built, including nursery standard nursery facility, device configuration and construction.This be seedling quickly breeding,
Shorten growing-seedling period, improve the facility guarantee of plant percent.
Cultivate after tissue culture transplantation of seedlings, including seedling separation specification, rich water quality management, go out the complete seedling technique for quickly cultivating such as garden standard
System.This is sprouting and rooting, improves the technical guarantee of seedling quality.
Compared with prior art, the invention has the beneficial effects as follows：
1st, the present invention defines complete set, brand-new oil tree peony quick reproduction technique system, and thinking is novel, and linking is closed
Reason, easily realizes, and the oil a variety of property holdings of tree peony new product, breeding efficiency can be greatly improved, and can reduce oil and be educated with tree peony
Seedling cost, improves breeding efficiency.
2nd, the present invention can improve rapidly the purity of oil tree peony new varieties, the quality of steady production oil peony seeds and commodity
Uniformity, improves crudy.
3rd, oil tree peony Clonal regeneration method for culturing seedlings of the present invention, than tree peony nursery stock tradition mating system, line of breeding
Number improves 3-5 times, and growing-seedling period shortens 20%, improves more than 15% into shoot survival percent.
Description of the drawings
Fig. 1 is the schematic flow sheet of oil tree peony Clonal regeneration method for culturing seedlings of the invention.
Specific embodiment
Embodiment 1
Oil tree peony Clonal regeneration method for culturing seedlings, comprises the steps：
(1) bulbil explant is selected：It is explant to select cane bottom bulbil, and the phase of drawing materials that bulbil explant is selected is 3
Month；
(2) break bulbil explant dormancy：Initially with the HgCl that mass concentration is 0.1%2Immersion 8min, then adopts
Agriculture number biological bactericide in mountain soaks twice, and each soak time is 15min；
(3) seedling tissue cultures：In culturing room, proliferated culture medium, root induction culture medium, root growth culture is adopted successively
Base is cultivated to the bulbil after step (2) process, obtains plantlet in vitro；
Proliferated culture medium is：MS+6-BA1.0mg/L+NAA0.5mg/L+3.5mg/LAgNO3；
Root induction culture medium is：WPM+IBA 6.0mg/L+ sucrose 20g/L；
Root growth culture medium is：WPM+IBA 6.0mg/L+ sucrose 40g/L；
(4) addressing in nursery：Nursery selects open level land on the sunny side, it is desirable to traffic convenience, has abundant water resources, totally, power supply
Convenient, away from epidemic-stricken area；
(5) construction of planting greenhouse：Planting greenhouse is the flat roof type booth of east-west height 2m, and frame is provided with shade
The shading screen for 75% is spent, hydropower installation is used in place afterwards；
(6) seedbed is built：Bed surface requires smooth, bedside 1.1m, seedbed spacing 55cm；The fertilizer for becoming thoroughly decomposed is loaded specification
For the nutrition cup of 12cm × 10cm, nutrition cup is placed in seedbed, 7d before transplanting is watered with 650 times of carbendazim solutions, film
Film is opened after covering 4d, after irrigating by seedling；
(7) tissue culture transplantation of seedlings nursery：
Plantlet in vitro is transferred in planting greenhouse from culturing room, 8d hardenings are placed, then, with clear water by the culture medium of root
Rinse well, then with planting in nutrition cup after the mixed solution immersion of 900 times of carbendazim and root-inducing powder, with punching with kind；
(8) seedling is cultivated after tissue culture transplantation of seedlings：
Plantlet in vitro is planted to after nutrition cup, and then seedbed plastic covering film 8d takes off film, planting greenhouse is hidden after taking off film
Cloudy；Take off film at the same pour permeable, see later dry just pour, watering every time irrigates, and plantlet in vitro grows into 5 plants/clump, when 12cm is high,
Change basin plant division；Plantlet in vitro grows into long more than the 4cm of branch, more than branch footpath 0.25cm, long more than the 10cm of root, during thick more than the 0.3cm of root,
Go out garden, listing.
Wherein, during plastic covering film, nocturnal temperature is kept for 20 DEG C or so, and the temperature on daytime is less than 30 DEG C.
The prevention and control of plant diseases, pest control is carried out after tissue culture transplantation of seedlings, and method is as follows：Carried out using 1000 times of carbendazim solution high-pressure fogs
The preventing and treating of disease；Control aphid, mite class is used alternatingly using 1000 times of liquid of 20% Fenpropathrin, 1000 times of liquid and 48% chlopyrifos
Harm.
Apply fertilizer after tissue culture transplantation of seedlings, method is as follows：Plantlet in vitro starts to apply fertilizer after extracting 2 young leaves out, and early stage mainly applies urea,
Concentration is 0.1%, and the later stage properly increases 0.2%-0.3% concentration, coordinates and applies a small amount of composite fertilizer.
Embodiment 2
Oil tree peony Clonal regeneration method for culturing seedlings, comprises the steps：
(1) bulbil explant is selected：It is explant to select cane bottom bulbil, and the phase of drawing materials that bulbil explant is selected is 4
Month；
(2) break bulbil explant dormancy：Initially with the HgCl that mass concentration is 0.1%2Immersion 9min, then adopts
Agriculture number biological bactericide in mountain soaks twice, and each soak time is 20min；
(3) seedling tissue cultures：In culturing room, proliferated culture medium, root induction culture medium, root growth culture is adopted successively
Base is cultivated to the bulbil after step (2) process, obtains plantlet in vitro；
Proliferated culture medium is：MS+6-BA1.0mg/L+NAA0.6mg/L+4mg/LAgNO3；
Root induction culture medium is：WPM+IBA 6.0mg/L+ sucrose 20g/L；
Root growth culture medium is：WPM+IBA 6.0mg/L+ sucrose 40g/L；
(4) addressing in nursery：Nursery selects open level land on the sunny side, it is desirable to traffic convenience, has abundant water resources, totally, power supply
Convenient, away from epidemic-stricken area；
(5) construction of planting greenhouse：Planting greenhouse is the flat roof type booth of east-west height 2m, and frame is provided with shade
The shading screen for 80% is spent, hydropower installation is used in place afterwards；
(6) seedbed is built：Bed surface requires smooth, bed 1.2m, seedbed spacing 60cm；By the fertilizer for becoming thoroughly decomposed loading specification it is
The nutrition cup of 12cm × 10cm, nutrition cup is placed in seedbed, 9d before transplanting, and is watered with 800 times of carbendazim solutions, film cover
Film is opened after upper 5d, after irrigating by seedling；
(7) tissue culture transplantation of seedlings nursery：
Plantlet in vitro is transferred in planting greenhouse from culturing room, 10d hardenings are placed, then, with clear water by the culture of root
Base is rinsed well, then with planting in nutrition cup after the mixed solution immersion of 1000 times of carbendazim and root-inducing powder, with punching with kind；
(8) seedling is cultivated after tissue culture transplantation of seedlings：
Plantlet in vitro is planted to after nutrition cup, and then seedbed plastic covering film 10d takes off film, planting greenhouse is hidden after taking off film
Cloudy；Take off film at the same pour permeable, see later dry just pour, watering every time irrigates, and plantlet in vitro grows into 6 plants/clump, when 15cm is high,
Change basin plant division；Plantlet in vitro grows into long more than the 4cm of branch, more than branch footpath 0.25cm, long more than the 10cm of root, during thick more than the 0.3cm of root,
Go out garden, listing.
Wherein, during plastic covering film, nocturnal temperature is kept for 15 DEG C, and the temperature on daytime is less than 30 DEG C.
The prevention and control of plant diseases, pest control is carried out after tissue culture transplantation of seedlings, and method is as follows：Entered using 1000 times of Mancozeb solution high-pressure fogs
The preventing and treating of row disease；Control aphid, mite is used alternatingly using 1000 times of liquid of 20% Fenpropathrin, 1000 times of liquid and 48% chlopyrifos
Class endangers.
Apply fertilizer after tissue culture transplantation of seedlings, method is as follows：Plantlet in vitro starts to apply fertilizer after extracting 2 young leaves out, and early stage mainly applies urea,
Concentration is 0.1%, and the later stage properly increases 0.2%-0.3% concentration.
Embodiment 3
Oil tree peony Clonal regeneration method for culturing seedlings, comprises the steps：
(1) bulbil explant is selected：It is explant to select cane bottom bulbil, and the phase of drawing materials that bulbil explant is selected is 4
Month；
(2) break bulbil explant dormancy：Initially with the HgCl that mass concentration is 0.1%2Immersion 7min, then adopts
Agriculture number biological bactericide in mountain soaks twice, and each soak time is 10min；
(3) seedling tissue cultures：In culturing room, proliferated culture medium, root induction culture medium, root growth culture is adopted successively
Base is cultivated to the bulbil after step (2) process, obtains plantlet in vitro；
Proliferated culture medium is：MS+6-BA1.0mg/L+NAA0.4mg/L+3mg/LAgNO3；
Root induction culture medium is：WPM+IBA 6.0mg/L+ sucrose 20g/L；
Root growth culture medium is：WPM+IBA 6.0mg/L+ sucrose 40g/L；
(4) addressing in nursery：Nursery selects open level land on the sunny side, it is desirable to traffic convenience, has abundant water resources, totally, power supply
Convenient, away from epidemic-stricken area；
(5) construction of planting greenhouse：Planting greenhouse is the flat roof type booth of east-west height 2m, and frame is provided with shade
The shading screen for 70% is spent, hydropower installation is used in place afterwards；
(6) seedbed is built：Bed surface requires smooth, bedside 1m, seedbed spacing 50cm；By the fertilizer for becoming thoroughly decomposed loading specification it is
The nutrition cup of 12cm × 10cm, nutrition cup is placed in seedbed, 7d before transplanting, and is watered with 500 times of carbendazim solutions, film cover
Film is opened after upper 3d, after irrigating by seedling；
(7) tissue culture transplantation of seedlings nursery：
Plantlet in vitro is transferred in planting greenhouse from culturing room, 7d hardenings are placed, then, with clear water by the culture medium of root
Rinse well, then with planting in nutrition cup after the mixed solution immersion of 800 times of carbendazim and root-inducing powder, with punching with kind；
(8) seedling is cultivated after tissue culture transplantation of seedlings：
Plantlet in vitro is planted to after nutrition cup, and then seedbed plastic covering film 7d takes off film, planting greenhouse is hidden after taking off film
Cloudy；Take off film at the same pour permeable, see later dry just pour, watering every time irrigates, and plantlet in vitro grows into 5 plants/clump, when 10cm is high,
Change basin plant division；Plantlet in vitro grows into long more than the 4cm of branch, more than branch footpath 0.25cm, long more than the 10cm of root, during thick more than the 0.3cm of root,
Go out garden, listing.
Wherein, during plastic covering film, nocturnal temperature is kept for more than 15 DEG C, and the temperature on daytime is less than 30 DEG C.
The prevention and control of plant diseases, pest control is carried out after tissue culture transplantation of seedlings, and method is as follows：Carried out using 1000 times of carbendazim solution high-pressure fogs
The preventing and treating of disease；Control aphid, mite class is used alternatingly using 1000 times of liquid of 20% Fenpropathrin, 1000 times of liquid and 48% chlopyrifos
Harm.
Apply fertilizer after tissue culture transplantation of seedlings, method is as follows：Plantlet in vitro starts to apply fertilizer after extracting 1-2 piece young leaves out, and fertilization type is multiple
Hefei, the mass concentration of fertilising is 0.1%-0.3%, and concentration of applying fertilizer is incremented by with the growth of plantlet in vitro.
Oil described in embodiment tree peony Clonal regeneration method for culturing seedlings, than tree peony nursery stock tradition mating system, breeding coefficient
3-5 times is improved, growing-seedling period shortens 20%, more than 15% is improved into shoot survival percent.
Claims (9)
1. a kind of oily with tree peony Clonal regeneration method for culturing seedlings, it is characterised in that：Comprise the steps：
(1) bulbil explant is selected：It is explant to select cane bottom bulbil；
(2) break bulbil explant dormancy：First bulbil is sterilized, is then soaked using biological bactericide；
(3) seedling tissue cultures：In culturing room, proliferated culture medium, root induction culture medium, root growth culture medium pair is adopted successively
Bulbil after step (2) process is cultivated, and obtains plantlet in vitro；
(4) addressing in nursery：Nursery selects open level land on the sunny side, it is desirable to traffic convenience, has abundant water resources, totally, and power supply is convenient,
Away from epidemic-stricken area；
(5) construction of planting greenhouse：Planting greenhouse be flat roof type booth, frame be provided with degree of shading be 70-80% shading screen,
Hydropower installation is used in place afterwards；
(6) seedbed is built：Bed surface requires smooth, bedside 1-1.2m, seedbed spacing 50-60cm；The fertilizer for becoming thoroughly decomposed is loaded rule
Lattice are placed in seedbed for the nutrition cup of 12cm × 10cm, at least 7d before transplanting, and are watered with 500-800 times of carbendazim solution, thin
Film is opened after 3-5d on membrane cover, after irrigating by seedling；
(7) tissue culture transplantation of seedlings nursery：
Plantlet in vitro is transferred in planting greenhouse from culturing room, 7-10d hardenings are placed, then, with clear water by the culture medium of root
Rinse well, then with planting in nutrition cup after the mixed solution immersion of 800-1000 times of carbendazim and root-inducing powder, with punching with kind；
(8) seedling is cultivated after tissue culture transplantation of seedlings：
Plantlet in vitro is planted to after nutrition cup, and then seedbed plastic covering film 7-10d takes off film, planting greenhouse is sheltered from heat or light after taking off film；
Take off film at the same pour permeable, see later dry just pour, watering every time irrigates, and plantlet in vitro grows into 5-6 strains/clump, and 10-15cm is high
When, change basin plant division；Plantlet in vitro grows into long more than the 4cm of branch, more than branch footpath 0.25cm, long more than the 10cm of root, thick more than the 0.3cm of root
When, go out garden, listing.
2. according to claim 1 oily with tree peony Clonal regeneration method for culturing seedlings, it is characterised in that：In step (1), outside bulbil
The phase of drawing materials that implant is selected is the 3-4 months.
3. according to claim 1 oily with tree peony Clonal regeneration method for culturing seedlings, it is characterised in that：In step (2), sterilizing is
Adopt mass concentration for 0.1% HgCl2Immersion 7-9min.
4. according to claim 1 oily with tree peony Clonal regeneration method for culturing seedlings, it is characterised in that：In step (2), using life
Thing bactericide soaks twice, and each soak time is 10-20min, and biological bactericide is mountain agriculture one.
5. according to claim 1 oily with tree peony Clonal regeneration method for culturing seedlings, it is characterised in that：In step (3), propagation training
Foster base is：MS+6-BA1.0mg/L+NAA0.4-0.6mg/L+3-4mg/LAgNO3；Root induction culture medium is：WPM+IBA
6.0mg/L+ sucrose 20g/L；Root growth culture medium is：WPM+IBA 6.0mg/L+ sucrose 40g/L.
6. according to claim 1 oily with tree peony Clonal regeneration method for culturing seedlings, it is characterised in that：In step (5), plantation is big
The trend of canopy is east-west.
7. according to claim 1 oily with tree peony Clonal regeneration method for culturing seedlings, it is characterised in that：In step (8), seedbed is covered
During lid plastic sheeting, nocturnal temperature is kept for more than 15 DEG C, and the temperature on daytime is less than 30 DEG C.
8. according to claim 1 oily with tree peony Clonal regeneration method for culturing seedlings, it is characterised in that：In step (8), plantlet in vitro
The prevention and control of plant diseases, pest control is carried out after transplanting, and method is as follows：Carried out using 1000 times of carbendazim solutions or the high-pressure fog of Mancozeb solution
The preventing and treating of disease；Control aphid, mite class is used alternatingly using 1000 times of liquid of 20% Fenpropathrin, 1000 times of liquid and 48% chlopyrifos
Harm.
9. according to claim 1 oily with tree peony Clonal regeneration method for culturing seedlings, it is characterised in that：In step (8), plantlet in vitro
Apply fertilizer after transplanting, method is as follows：Plantlet in vitro starts to apply fertilizer after extracting 1-2 piece young leaves out, and fertilization type is in urea or composite fertilizer
One or two mixture, the mass concentration of fertilising is 0.1%-0.3%, and concentration of applying fertilizer is incremented by with the growth of plantlet in vitro.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108094197A (en) * | 2017-11-30 | 2018-06-01 | 安徽心缘康生物科技有限公司 | A kind of oil tree peony phoenix pellet asexual multiplication seedling method |
| CN108935100A (en) * | 2018-07-20 | 2018-12-07 | 河南农业大学 | A kind of method of apple rootstock T337 tissue-cultured seedling rooting induction |
| CN111727879A (en) * | 2019-07-11 | 2020-10-02 | 中国科学院植物研究所 | Culture medium and method for oil peony propagation |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102217543A (en) * | 2011-05-24 | 2011-10-19 | 崔刚 | Open plant tissue culture and factory rapid propagation method |
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| CN108935100A (en) * | 2018-07-20 | 2018-12-07 | 河南农业大学 | A kind of method of apple rootstock T337 tissue-cultured seedling rooting induction |
| CN108935100B (en) * | 2018-07-20 | 2021-09-28 | 河南农业大学 | Apple rootstock T337 tissue culture seedling rooting induction method |
| CN111727879A (en) * | 2019-07-11 | 2020-10-02 | 中国科学院植物研究所 | Culture medium and method for oil peony propagation |
| CN111727879B (en) * | 2019-07-11 | 2021-10-15 | 中国科学院植物研究所 | Culture medium and method for oil peony propagation |
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