CN103583360B - A kind of directional induction improves the method for Abelia biflora nursery stock salt resistance - Google Patents
A kind of directional induction improves the method for Abelia biflora nursery stock salt resistance Download PDFInfo
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Abstract
The invention discloses a kind of method that directional induction improves Abelia biflora nursery stock salt resistance, comprise the following steps: transplant seedlings in the induction of (1) material sterilizing, (2) indefinite bud, the differentiation of (3) bud and squamous subculture, (4) culture of rootage, (5) hardening, (6), (7) seedling management.The present invention utilizes tissue cultures and container nursery means, the NaCl of variable concentrations is progressively added in differentiation and increment medium, root media and matrix that after transplanting seedlings, nursery stock is cultivated, make the salt resistance of nursery stock from about 0.2% bringing up to about 0.6% (one year seedling matrix NaCl content 0.4-0.5% can normal growth, NaCl content 0.5-0.6% can normal growth in biennial seedling matrix), be enhanced about more than once at the planting survival rates in saline land.
Description
Technical field
The present invention relates to ornamental trees and shrubs Cultivating techniques field, specifically refer to utilize modern seedling growing process means to obtain the method for Abelia biflora nursery stock raising Saline alkali tolerance.
Background technology
Abelia biflora originates in China, is Caprifoliaceae Abelia plant, machaka, is the foremost color leaf ornamental tree species of China.Abelia biflora has stronger adaptability, low temperature resistant, and resistance to soil is barren, has certain Saline alkali tolerance and Drought resistance.Abelia biflora is one of important Landscape Trees of China's southeastern coast, but southeastern coast is located in subtropics, and annual rainfall is large, and drench salt by rainwater and desalinate salinization soil gradually, salt is grouped into based on chloride, and salt content is 0.1%-0.8%.
Summary of the invention
Though the salt resistance that Abelia biflora tool is certain, the present inventor research team years of researches confirm, soil salt content is Abelia biflora growth retardation more than 0.2%, and 0.3% is the threshold values that its grows.The breeding of Abelia biflora generally takes cuttage, grow directly from seeds and the method such as tissue cultures, test shows, if adopt cuttage and Seedling propagation, the salt content progressively increased in seedling medium, also the salt resistance of nursery stock can be improved, but by the means of tissue cultures and Fast-propagation, the matrix from the matrix of tissue cultures to nursery increases salt content gradually, the nursery stock that the nursery stock salt resistance of producing obtains higher than cuttage and Seedling propagation.The present invention is based on the theory that plant is progressively tamed, from differential period of tissue cultures bud to the nursery stock stage, improved the salt resistance of Abelia biflora by manual intervention domestication.
Technical problem to be solved by this invention is the situation high for ground, existing China's southeastern coast tidal land seabeach salt content, Abelia biflora seedling planting survival rate is low, provides a kind of directional induction to improve the method for Abelia biflora nursery stock salt resistance.The present invention utilizes tissue cultures and container nursery means, the NaCl of variable concentrations is progressively added in differentiation and increment medium, root media and matrix that after transplanting seedlings, nursery stock is cultivated, make the salt resistance of nursery stock from about 0.2% bringing up to about 0.6% (one year seedling matrix NaCl content 0.4-0.5% can normal growth, NaCl content 0.5-0.6% can normal growth in biennial seedling matrix), be enhanced about more than once at the planting survival rates in saline land.
The present invention solves the problems of the technologies described above adopted technical scheme: the method comprises the induction of Abelia biflora bud, differentiation and proliferation is cultivated, the induction of root system and the cultivation etc. of nursery stock,
(1) material sterilizing: get Abelia biflora edible tender branch, is cut into 4-5cm stem segment with axillary bud, first in cleaning solution, soaks 5-6 minute, then cuts off the blade of expansion after rinsing 2-3 hour with water, is cut into the stem segment with axillary bud of 1.5cm; Superclean bench soaks 20 seconds with 75% alcoholic solution, and then proceeding to mass fraction is sterilizing 8-10 minute in the mercuric chloride solution of 0.1%, then uses aseptic water washing 5-8 time;
(2) induction of indefinite bud: the stem section after described step (1) sterilizing is received on No. 1 medium and cultivate 40-50 days;
Wherein, described No. 1 medium adds the NaCl that mass fraction is 0.1% on MS medium base;
(3) differentiation of bud and squamous subculture: select the good stem section of Bud polarization to receive on No. 2 medium, the bud that growth selection is good squamous subculture on same described No. 2 medium of transferring once, is then transferred on No. 3 medium and cultivated for 2 generations; Multiple Buds is there is after a few culture;
Wherein, described No. 2 medium add the NaCl that mass fraction is 0.1% on MS medium base; Described No. 3 medium add the NaCl that mass fraction is 0.2% on MS medium base;
(4) culture of rootage: choose well-grown Multiple Buds and be divided into individual plant, goes to root induction on No. 4 medium; Cultivate 15-20 days, root system more than 5, height of seedling 4-6cm transplants seedlings; Described No. 4 medium add the NaCl that mass fraction is 0.3% on the basis of 1/2MS medium;
(5) hardening: the bottleneck of well-grown seedling of taking root is opened, at room temperature uncork hardening 2-3 days, by test-tube plantlet take out, after rinsing out root medium with clear water, be transplanted to be provided with sea sand husky bed on carry out hardening; Described sea sand salt content 0.4%; During hardening, distance between rows and hills is 10 × 10cm; New root grows and is transplanted in container and cultivates after extraction young leaves; Salt content in nurturing period chien shih sea sand is held constant at 0.4%;
(6) transplant seedlings: within 1 year, raw Abelia biflora container substrate composition is peat according to volume ratio: sea sand=7:3, slow-release compound fertilizer 1kgm
-3; Within 2 years, raw Abelia biflora container substrate composition is peat according to volume ratio: sea sand=6:4, slow-release compound fertilizer 2kg.m
-3; Add NaCl during preparation matrix, the NaCl content in one year seedling matrix is 0.5%, and the NaCl content in biennial seedling matrix is 0.6%;
(7) seedling management: container seedling is placed in beam-connected greenhouse, the temperature in beam-connected greenhouse remains on 20-25 DEG C, and relative moisture of the soil remains on 65-75%.
In described step (2), described No. 1 medium also adds following material: the kinetin of 0.8-1.0mg/L, the indolebutyric acid of 0.08-0.12mg/L and mass fraction are the sucrose of 3%.
In described step (3), describedly contain No. 2 medium and also add following material: methyl α-naphthyl acetate and the mass fraction of the kinetin of 0.4-0.6mg/L, the 6-benzyl aminoadenine of 0.08-0.12mg/L, 0.05mg/L are the sucrose of 3%.Wherein, mg/L represents the material milligram number contained in often liter of medium.
In described step (3), describedly contain No. 3 medium and also add following material: methyl α-naphthyl acetate and the mass fraction of the kinetin of 0.4-0.6mg/L, the 6-benzyl aminoadenine of 0.08-0.12mg/L, 0.04-0.06mg/L are the sucrose of 3%.
In described step (4), described No. 4 medium also add following material: the indolebutyric acid of 0.4-0.6mg/L and mass fraction are the sucrose of 2%.
The condition of culture of described step (2), step (3) and step (4) is 24-25 DEG C, relative air humidity 70-75%, light intensity 2500-3000Lx, illumination 10 hours/day.
In described step (5), be the sunshade net of 50% with light transmittance within transplanting seedlings latter 10 days.
In described step (5), described husky bed from bottom to top comprises bottom, intermediate layer and upper strata; The earth that the cobble that described bottom is 8-15cm, described intermediate layer are 5cm, described upper strata are the sea sand of 20cm.
In described step (6), within 1 year, raw container for plant growth uses material mesh bag container, and diameter is 4-5cm, height 8-10cm; Within 2 years, raw container for plant growth uses material mesh bag container or plastic containers, and diameter is 18-20cm, is highly 300-310cm.
In described step (7), within the 2nd year, carry out changing basin according to the upgrowth situation of nursery stock, select material mesh bag container or plastic containers, diameter is 18-20cm, is highly 300-310cm later, basin soil amount 800-1000g.
In described step (7), the spraying facility of beam-connected greenhouse is utilized to spray.
In described step (7), carry out sunshade during summer high temperature to container seedling seedling, sunshade light transmittance is 60% of full exposure.
Described step (5), step (6) and step (7) period air themperature is more than or equal to 18 DEG C, soil matrix temperature is not less than 15 DEG C.
Explanation of nouns
Cleaning solution: refer to washing powder mass percentage concentration 0.4% ~ 1% detergent solution, described washing powder can be washing powder market can being purchased available common brand, and described washing powder also can replace with neopelex.
MS medium: composition is as shown in table 1
The basic ingredient table of table 1:MS medium
1/2MS medium: refer to that, on the basis of above-mentioned MS medium, the concentration of macroelement reduced by half, all the other compositions remain unchanged.
Salt content: " salt content " mentioned in the application refers to the mass percentage concentration of NaCl.
Content: " content " mentioned in the application refers to mass percentage concentration.
Material mesh bag container: the container that the poroid material of degradable fleece referred to is made.
Plastic containers: refer to the container that plastic material is made.
Removable plastic containers: be cultivating container common in nursery stock cultivation technique without soil, can buy approach by business and obtain.Its surrounding is cellular, and during nursery, container two ends draw-in groove is fixed, and container is cave-shaped, and when field planting, draw-in groove is removed, and container is flat condition.
Basin soil amount: the matrix weight contained in a container.
Advantage of the present invention and beneficial effect:
1. the present invention successfully adopts tissue cultures and container nursery means, progressively increases salt content and carry out salt resistance domestication in matrix, obtains its salt resistance of nursery stock and improves more than 60% than conventional seedbed system.The present invention to obtain in the one year seedling matrix of Abelia biflora NaCl content 0.4%-0.5% can normal growth, NaCl content 0.5%-0.6% can normal growth in biennial seedling matrix.
2. the present invention is by determining the induction of Abelia biflora bud, differentiation, subculture and culture of rootage and hardening, the matrix formulations migrated in container, progressively increases the salt content in matrix, obtains the raising Abelia biflora Salt And Alkali Tolerance seedling-cultivating method of complete set.Key of the present invention be salt content in the configuration of each stage medium and medium control and each in stage plant screening.
3. although the means of the present invention by progressively taming, obtain the high nursery stock of salt resistance, but do not mean utilize this nursery stock for female parent carry out follow-on Sterile culture (as cuttage, grafting and seminal propagation) obtain nursery stock there is higher salt resistance.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but the present invention is not only confined to following examples.
Embodiment 1 improves Abelia biflora " Edward " " (Abelia'EdwardGoucher') method that is salt resistance
1. materials disinfection: get Abelia biflora " Edward " edible tender branch, be cut into 4-5cm stem segment with axillary bud, first in the cleaning solution (cleaning solution refers to the aqueous solution containing mass fraction about 0.5% neopelex) being added with washing powder, soak 5-6 minute, again with the blade cutting off expansion after tap water 2-3 hour, be cut into 1.5cm left and right belt leaf stem section.On superclean bench, soak 20 seconds with 75% alcoholic solution, then proceeding to mass fraction is sterilizing 8-10 minute in the mercuric chloride solution of 0.1%, and with aseptic water washing 5-8 time.
2. the induction of indefinite bud: the stem section of sterilizing is received MS+ kinetin 1.0mg/L+ indolebutyric acid 0.1mg/L+ mass fraction be 3% sucrose+mass fraction be the NaCl of 0.1% medium on cultivate 40-50 days.Constantly the well-grown stem section of Bud polarization is screened in the Induction Process of bud.
3. the differentiation of bud and squamous subculture: select the good stem section of Bud polarization receive MS+6-benzyl aminoadenine 0.1mg/L+ kinetin 0.5mg/L+ methyl α-naphthyl acetate 0.05mg/L+ mass fraction be 3% sucrose+mass fraction be on the proliferated culture medium of the NaCl of 0.1%, the bud that growth selection is good transfer same medium (MS+6-benzyl aminoadenine 0.1mg/L+ kinetin 0.5mg/L+ methyl α-naphthyl acetate 0.05mg/L+ mass fraction be 3% sucrose+mass fraction be the NaCl of 0.1%) upper squamous subculture once, be then transferred to MS+6-benzyl aminoadenine 0.1mg/L+ kinetin 0.5mg/L+ methyl α-naphthyl acetate 0.05mg/L+ mass fraction be 3% sucrose+mass fraction be the NaCl of 0.2% medium on cultivated for 2 generations.Multiple Buds is there is after a few culture.
4. culture of rootage: choose well-grown Multiple Buds and be divided into individual plant, go to 1/2MS+ indolebutyric acid 0.5mg/L+ mass fraction be 2% sucrose+mass fraction be 0.3% NaCl root media on root induction.Cultivate 15-20 days, root system more than about 5, height of seedling 4-6cm transplants seedlings.
More than group training room condition of culture be about 25 DEG C, relative air humidity about 70%, about light intensity 2500Lx, illumination 10 hours/day.
5. hardening: the plantlet in vitro eliminating growth difference, opens the bottleneck of well-grown seedling of taking root, at room temperature uncork hardening 2-3 days, is taken out by test-tube plantlet, after rinsing out root medium, is transplanted on husky bed and carries out hardening with clear water.The sea sand (sea sand salt content about 0.4%) that husky bed bottom is the cobble of about 10cm, the middle earth for about 5cm, upper strata are about 20cm.During hardening, distance between rows and hills is 10 × 10cm, carries out automatic irrigation aperiodically according to soil and nursery stock leaf wetness, keeps the moistening not dehydration of nursery stock blade.Progressively reduce the humidity of husky bed after new root grows, be transplanted in container after extraction young leaves and cultivate.Period notices that the salt content in matrix keeps constant substantially, adds appropriate NaCl when salt content reduces in matrix in sprinkling irrigation water.
6. transplant seedlings: with sunshade net (light transmittance about 50%) within transplanting seedlings latter about 10 days.Within 1 year, raw container for plant growth material mesh bag container diameter is 4-5cm, height 8-10cm; 2 years raw container for plant growth material mesh bag containers and removable plastic containers diameter are 18-20cm, are highly 300-310cm.General Abelia biflora 1 year raw chamber test matrix is peat according to volume ratio proportioning: sea sand=7:3, slow-release compound fertilizer (1kgm-3); Within 2 years, raw container matrix is peat according to volume ratio proportioning: sea sand=6:4, slow-release compound fertilizer (2kg.m-3).For prevention nursery stock generation damage by disease and insect, matrix palpus strict sterilization, add NaCl during preparation matrix, the NaCl content in one year seedling matrix is about 0.4%, and the NaCl content in biennial seedling matrix is about 0.5%.
7. seedling management: container seedling is placed in the beam-connected greenhouse of configuration sunshade and spraying facility, and the temperature in booth remains on 20-25 DEG C, and relative moisture of the soil remains on 65-75%.Within 2nd year, carry out changing basin according to the upgrowth situation of nursery stock, when biennial seedling matrix changes basin, matrix is sea sand=6:4 according to volume ratio proportioning, slow-release compound fertilizer (2kg.m-3) later; NaCl content in biennial seedling matrix is 0.5%.The container used can be material mesh bag container or removable plastic containers, and diameter is 18-20cm, is highly 300-310cm, basin soil amount 800-1000g.To fill the gaps with seedlings in time to being short of seedling or growing poor container seedling.To water immediately after filling the gaps with seedlings.Adopt to spray in large-scale production into good, beam-connected greenhouse spraying facility can be utilized to spray.Pour water according to the change of synoptic season temp. and humidity, water and will irrigate, the Best Times that waters is morning or dusk.
Above hardening, to transplant seedlings and between nursery stage, air themperature is not less than 18 DEG C, soil (matrix) temperature is not less than 15 DEG C.
Slow-release compound fertilizer is applied in matrix, generally without the need to refertilizing, but can in conjunction with suitable top dressing of pouring water according to container seedling growth situation.Need during summer high temperature to carry out sunshade to container seedling seedling, sunshade light transmittance is about 60% of full exposure.Occur if any disease, remove diseased plant in time, and use corresponding pesticide spraying sterilizing.As damaged by vermin occurs, prevent and treat in time.Find that in container, matrix is sunk, and must add matrix in time, prevent root exposed seedling stage.In seedling management, also want the salt content in nota bone matrix substantially to keep constant, when salt content reduces in matrix, in sprinkling irrigation water, add appropriate NaCl.
Facts have proved: soil salt content is Abelia biflora " Edward " growth retardation more than 0.2%, 0.3% is the threshold values that its grows.After the method process of the present embodiment 1, in the one year seedling matrix of Abelia biflora " Edward ", in NaCl content 0.4% energy normal growth, biennial seedling matrix, NaCl content 0.5% can normal growth.
Embodiment 2 improves the method for floral leaf Abelia biflora (formal name used at school: Abeliagrandiflora " FrancisMason ") salt resistance
1. materials disinfection: get floral leaf Abelia biflora edible tender branch, be cut into 4-5cm stem segment with axillary bud, first in the cleaning solution (cleaning solution refers to the aqueous solution of the neopelex containing mass fraction 0.5%) being added with washing powder, soak 5-6 minute, cut off the blade of expansion again with tap water after 3 hours, be cut into the stem with bud of 1.5cm.On superclean bench, soak 20 seconds with 75% alcoholic solution, then proceeding to mass fraction is sterilizing 8-10 minute in the mercuric chloride solution of 0.1%, and with aseptic water washing 6-8 time.
2. the induction of indefinite bud: the stem section of sterilizing is received MS+ kinetin 1.0mg/L+ indolebutyric acid 0.1mg/L+ mass fraction be 3% sucrose+mass fraction be the NaCl of 0.1% medium on cultivate 40-50 days.Constantly the well-grown stem section of Bud polarization is screened in the Induction Process of bud.
3. the differentiation of bud and squamous subculture: select the good stem section of Bud polarization receive MS+6-benzyl aminoadenine 0.1mg/L+ kinetin 0.5mg/L+ methyl α-naphthyl acetate 0.05mg/L+ mass fraction be 3% sucrose+mass fraction be on the proliferated culture medium of the NaCl of 0.1%, the bud that growth selection is good transfer same medium (MS+6-benzyl aminoadenine 0.1mg/L+ kinetin 0.5mg/L+ methyl α-naphthyl acetate 0.05mg/L+ mass fraction be 3% sucrose+mass fraction be the proliferated culture medium of the NaCl of 0.1%) upper squamous subculture once, be then transferred to MS+6-benzyl aminoadenine 0.1mg/L+ kinetin 0.5mg/L+ methyl α-naphthyl acetate 0.05mg/L+ mass fraction be 3% sucrose+mass fraction be the NaCl of 0.2% medium on cultivated for 2 generations.Multiple Buds is there is after a few culture.
4. culture of rootage: choose well-grown Multiple Buds and be divided into individual plant, go to 1/2MS+ indolebutyric acid 0.5mg/L+ mass fraction be 2% sucrose+mass fraction be 0.3% NaCl root media on root induction.Cultivate 15-20 days, root system more than about 5, height of seedling 4-6cm transplants seedlings.
More than group training room condition of culture be 25 DEG C, relative air humidity 75%, light intensity 3000Lx, illumination 10 hours/day.
5. hardening: the plantlet in vitro eliminating growth difference, opens the bottleneck of well-grown seedling of taking root, at room temperature uncork hardening 2-3 days, is taken out by test-tube plantlet, after rinsing out root medium, is transplanted on husky bed and carries out hardening with clear water.The sea sand (sea sand salt content about 0.4%) that husky bed bottom is the cobble of about 10cm, the middle earth for about 5cm, upper strata are about 20cm.During hardening, distance between rows and hills is 10 × 10cm, carries out automatic irrigation aperiodically according to soil and nursery stock leaf wetness, keeps the moistening not dehydration of nursery stock blade.New root is transplanted in container after extracting young leaves out and cultivates.Period notices that the salt content in matrix keeps constant substantially, adds appropriate NaCl when salt content reduces in matrix in sprinkling irrigation water.
6. transplant seedlings: with sunshade net (light transmittance about 50%) within transplanting seedlings latter 10 days.Within 1 year, raw container for plant growth material mesh bag container diameter is 4-5cm, height 8-10cm; 2 years raw container for plant growth material mesh bag containers and removable plastic containers diameter are 18-20cm, are highly 300-310cm.Abelia biflora 1 year raw chamber test matrix is peat according to volume ratio proportioning: sea sand=7:3, slow-release compound fertilizer (1kgm-3); Within 2 years, raw container matrix is peat according to volume ratio proportioning: sea sand=6:4, slow-release compound fertilizer (2kg.m-3).For prevention nursery stock generation damage by disease and insect, matrix palpus strict sterilization, add NaCl during preparation matrix, the NaCl content in one year seedling matrix is 0.5%, and the NaCl content in biennial seedling matrix is 0.6%.
7. seedling management: container seedling is placed in the beam-connected greenhouse of configuration sunshade and spraying facility, and the temperature in booth remains on 20-25 DEG C, and relative moisture of the soil remains on 65-75%.Within 2nd year, carry out changing basin according to the upgrowth situation of nursery stock, when biennial seedling matrix changes basin, matrix is sea sand=6:4 according to volume ratio proportioning, slow-release compound fertilizer (2kg.m-3) later; NaCl content in biennial seedling matrix is 0.6%.The container used is material mesh bag container, and diameter is 18-20cm, is highly 300-310cm, basin soil amount 800-1000g.
Above hardening, to transplant seedlings and between nursery stage, air themperature is 18 DEG C, soil (matrix) temperature is 15 DEG C.
Slow-release compound fertilizer is applied in matrix, generally without the need to refertilizing, but can in conjunction with suitable top dressing of pouring water according to container seedling growth situation.Need during summer high temperature to carry out sunshade to container seedling seedling, sunshade light transmittance is about 60% of full exposure.Occur if any disease, remove diseased plant in time, and use corresponding pesticide spraying sterilizing.As damaged by vermin occurs, prevent and treat in time.Find that in container, matrix is sunk, and must add matrix in time, prevent root exposed seedling stage.In seedling management, also want the salt content in nota bone matrix substantially to keep constant, when salt content reduces in matrix, in sprinkling irrigation water, add appropriate NaCl.
Facts have proved: soil salt content is floral leaf Abelia biflora growth retardation more than 0.2%, 0.3% is the threshold values that its grows.After the method process of the present embodiment 1, in the one year seedling matrix of floral leaf Abelia biflora, in NaCl content 0.5% energy normal growth, biennial seedling matrix, NaCl content 0.6% can normal growth.
As mentioned above, just the present invention can be realized preferably.
Claims (8)
1. directional induction improves a method for Abelia biflora nursery stock salt resistance, it is characterized in that: comprise the following steps:
(1) material sterilizing: get Abelia biflora edible tender branch, is cut into 4-5cm stem segment with axillary bud, first in cleaning solution, soaks 5-6 minute, then cuts off the blade of expansion after rinsing 2-3 hour with water, is cut into the stem segment with axillary bud of 1.5cm; Superclean bench soaks 20 seconds with 75% alcoholic solution, and then proceeding to mass fraction is sterilizing 8-10 minute in the mercuric chloride solution of 0.1%, then uses aseptic water washing 5-8 time;
(2) induction of indefinite bud: the stem section after described step (1) sterilizing is received on No. 1 medium and cultivate 40-50 days;
Wherein, described No. 1 medium adds the NaCl that mass fraction is 0.1% on MS medium base;
(3) differentiation of bud and squamous subculture: select the good stem section of Bud polarization to receive on No. 2 medium, the bud that growth selection is good squamous subculture on same described No. 2 medium of transferring once, is then transferred on No. 3 medium and cultivated for 2 generations; Multiple Buds is there is after a few culture;
Wherein, described No. 2 medium add the NaCl that mass fraction is 0.1% on MS medium base; Described No. 3 medium add the NaCl that mass fraction is 0.2% on MS medium base;
(4) culture of rootage: choose well-grown Multiple Buds and be divided into individual plant, goes to root induction on No. 4 medium; Cultivate 15-20 days, root system more than 5, transplants seedlings during height of seedling 4-6cm; Described No. 4 medium add the NaCl that mass fraction is 0.3% on the basis of 1/2MS medium;
(5) hardening: the bottleneck of well-grown seedling of taking root is opened, at room temperature uncork hardening 2-3 days, by test-tube plantlet take out, after rinsing out root medium with clear water, be transplanted to be provided with sea sand husky bed on carry out hardening; Described sea sand salt content 0.4%; During hardening, distance between rows and hills is 10 × 10cm; New root grows and is transplanted in container and cultivates after extraction young leaves; Salt content in nurturing period chien shih sea sand is held constant at 0.4%;
(6) transplant seedlings: within 1 year, raw Abelia biflora container substrate composition is peat according to volume ratio: sea sand=7:3, slow-release compound fertilizer 1kgm
-3; Within 2 years, raw Abelia biflora container substrate composition is peat according to volume ratio: sea sand=6:4, slow-release compound fertilizer 2kg.m
-3; Add NaCl during preparation matrix, the NaCl content in one year seedling matrix is 0.5%, and the NaCl content in biennial seedling matrix is 0.6%;
(7) seedling management: container seedling is placed in beam-connected greenhouse, the temperature in beam-connected greenhouse remains on 20-25 DEG C, and relative moisture of the soil remains on 65-75%;
In described step (2), described No. 1 medium also adds following material: the kinetin of 0.8-1.0mg/L, the indolebutyric acid of 0.08-0.12mg/L and mass fraction are the sucrose of 3%;
In described step (3), describedly contain No. 2 medium and also add following material: methyl α-naphthyl acetate and the mass fraction of the kinetin of 0.4-0.6mg/L, the 6-benzyl aminoadenine of 0.08-0.12mg/L, 0.05mg/L are the sucrose of 3%;
In described step (3), describedly contain No. 3 medium and also add following material: methyl α-naphthyl acetate and the mass fraction of the kinetin of 0.4-0.6mg/L, the 6-benzyl aminoadenine of 0.08-0.12mg/L, 0.04-0.06mg/L are the sucrose of 3%;
In described step (4), described No. 4 medium also add following material: the indolebutyric acid of 0.4-0.6mg/L and mass fraction are the sucrose of 2%.
2. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: the condition of culture of described step (2), step (3) and step (4) is 24-25 DEG C, relative air humidity 70-75%, light intensity 2500-3000Lx, illumination 10 hours/day.
3. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: in described step (5), is the sunshade net of 50% with light transmittance within transplanting seedlings latter 10 days.
4. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, and it is characterized in that: in described step (5), described husky bed from bottom to top comprises bottom, intermediate layer and upper strata; The earth that the cobble that described bottom is 8-15cm, described intermediate layer are 5cm, described upper strata are the sea sand of 20cm.
5. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: in described step (6), and within 1 year, raw container for plant growth uses material mesh bag container, and diameter is 4-5cm, height 8-10cm; Within 2 years, raw container for plant growth uses material mesh bag container or plastic containers, and diameter is 18-20cm, is highly 300-310cm.
6. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: in described step (7), within 2nd year, carry out changing basin according to the upgrowth situation of nursery stock later, select material mesh bag container or plastic containers, diameter is 18-20cm, be highly 300-310cm, basin soil amount 800-1000g.
7. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: in described step (7), utilizes the spraying facility of beam-connected greenhouse to spray.
8. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, and it is characterized in that: in described step (7), carry out sunshade during summer high temperature to container seedling, sunshade light transmittance is 60% of full exposure.
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