CN104642073A - Leaf micro-cuttage propagation technology for rare and endangered plant Begonia coptidifolia - Google Patents
Leaf micro-cuttage propagation technology for rare and endangered plant Begonia coptidifolia Download PDFInfo
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- CN104642073A CN104642073A CN201310582499.7A CN201310582499A CN104642073A CN 104642073 A CN104642073 A CN 104642073A CN 201310582499 A CN201310582499 A CN 201310582499A CN 104642073 A CN104642073 A CN 104642073A
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- begonia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Abstract
The invention discloses a leaf micro-cuttage propagation technology for the rare and endangered plant Begonia coptidifolia. According to the invention, innovation on the size of a cutting is realized, a propagation coefficient is greatly improved, and the technology is the first research report about leaf cuttage propagation of the rare and endangered plant Begonia coptidifolia; the technology can be carried out in an appropriate temperature range of 20 to 30 DEG C; perlite, vermiculite or other matrixes are used for leaf micro-cuttage propagation of the rare and endangered plant Begonia coptidifolia in China; and a cutting with a size of as small as 0.5 cm can undergo normal differentiation and development and grow into a seedling. The technology makes a breakthrough to the conventional concept about the size limit of cuttings, greatly improves the propagation coefficient, is improvement and innovation of asexual reproductive technology and is the first research report about leaf cuttage propagation of the rare and endangered plant Begonia coptidifolia. The technology is also applicable to begonia and other plant species suitable for leaf cuttage propagation.
Description
Technical field
The present invention relates to a kind of rare or endangered species " begonia in spring " (
begonia coptidifolia) the micro-slotting propagation technique of blade.
Background technology
Spring begonia (
begonia coptidifolia) be only one many recurrent lobes kind in domestic Begonia plant, there is very important scientific research value.This kind of very narrow limitation of distribution; only on the part stone of several the Qing Gou in E Huangzhang Provincial Nature Reserve, Yangchun City, Guangdong Province, there is a small amount of distribution; the very special fragility in habitat, is therefore listed in national rare endangered plants, is also one of focused protection species of this nature reserve.Therefore, this kind is carried out introduce a fine variety, amount reproduction, in situ conservation and the Research Significance such as recurrence is great naturally.For this reason, we have carried out research to the reproductive biology of this kind in the world first, although find that seed propagation can be sprouted, emergence rate is not high, and seedling early growth is very slow.In addition, due to seed the fruit maturation such as to only have after could gather, seriously restrict by the time.And use blade cottage propagation instead; not only overcome time restriction; just a large amount of nursery stock can be bred due to available a small amount of blade material with in the minimum cuttings short time; serve the effect that allied organization cultivates; do not need to gather plant thus the destruction of at utmost reducing wild resource; not only solve the Fast-propagation problem of this kind, the protection and the field that are also conducive to this species wild resource return.The method introducing a fine variety protection and studying very important other kind of Begonia; as long as gather the least possible blade under not having seed-bearing situation in the wild to carry out micro-slotting breeding and just can hit pay dirk and introduce a fine variety object, also can promote and should produce in the nursery stock Fast-propagation of the high begonia commercial variety of ornamental value.
Summary of the invention
The object of the invention is to solve rare or endangered species " begonia in spring " in prior art (
begonia coptidifolia) rely on seminal propagation, the collection of seed is very difficult, and it is very low to there is seed germination rate, cannot meet rare or endangered species self Fast-propagation and expand the problem of cultivated area, provide a kind of rare or endangered species " begonia in spring " (
begonia coptidifolia) the micro-slotting propagation technique of blade.The present invention design a kind of rare or endangered species " begonia in spring " (
begonia coptidifolia) the micro-slotting propagation technique of blade, it is characterized in that, comprise the following steps:
Step one, choose begonia in ripe disease-free spring (
begonia coptidifolia) blade makes cuttings material;
Step 2, is cut into square cuttings with the knife blade of sterilization by blade, and with 70% thiophanate methyl wetting powder of dilution 800 times and the 50% carbendazol wettable powder solution equal-volume mixed liquid dipping sterilization in ten minutes of diluting 800 times;
Step 3, cuttage: hold cutting medium with the seedling-raising box of clean, upper transparent or other closed containers in greenhouse, by cuttings oblique cutting or be laid in cutting medium surface, vinyl cover or film on cuttage bonnet, cover, make regular check on and spray the suitable humidity of clean water maintenance; Step 4, upper basin: will root system be formed and basin in the cuttings growing seedling, and carry out normal cultivation management.In step 2: with the knife blade of sterilization, blade is cut into the size that square cuttings is of a size of 0.5-2 centimeter square.In step 3: cutting medium can with pure perlite, pure vermiculite, perlite and vermiculite mixture, river sand or other common cultivation matrixes, make cutting medium with pure perlite, pure vermiculite, perlite and vermiculite mixture, river sand not few containing germ in organic principle, matrix because of it, thus contribute to improving cuttings motility rate and reproduction rate.In step 3: cuttage environment makes regular check on water spray under will keeping more than 80% higher air humidity and light scattering environment, keep matrix moistening, and keep cuttage environmental temperature 20-30 DEG C.Advantage of the present invention is and substantially increases reproduction coefficient: cuttage respectively forms motility rate and is up to 91.66%, and minimum is 75%, and average survival is 81.96%.Seedling speed is the same with the cuttage of routine large cuttings.The method not only breaches the cuttings size limit that people's conventional thought is assert, also substantially increase reproduction coefficient, possesses the high efficiency of method for tissue culture, and overall process does not all require gnotobasis, being the improvement and bring new ideas of vegetative propagation technique, is also the report of research first of relevant endangered species " begonia in spring " blade cottage propagation.This method can be applicable to Begonia and other can insert the floristics of breeding by leaf.
Accompanying drawing explanation
Accompanying drawing 1 rare and endangered species " begonia in spring " and habitat thereof.
Accompanying drawing 2 rare and endangered species " begonia in spring " monolithic compound leaf.
The leaflet of accompanying drawing 3 pruning of the present invention and cuttings.
Accompanying drawing 4 cuttage of the present invention 1st day, 12 oblique cuttings, 13 be laid in vermiculite surface.
The situation of taking root of accompanying drawing 5 cuttage of the present invention cuttings after 43 days.
Bud polarization and seedling developmental state after 110 days after accompanying drawing 6 cuttage of the present invention.
The seedling that the micro-slotting breeding of accompanying drawing 7 blade of the present invention is formed.
The seedling situation of healthy growth after accompanying drawing 8 transplanting of the present invention.
Embodiment
A kind of rare or endangered species " begonia in spring " (
begonia coptidifolia) the micro-slotting propagation technique of blade, it is characterized in that, comprise the following steps:
Step one, choose begonia in ripe disease-free spring (
begonia coptidifolia) blade makes cuttings material;
Step 2, is cut into square cuttings with the knife blade of sterilization by blade, and with 70% thiophanate methyl wetting powder of dilution 800 times and the 50% carbendazol wettable powder solution equal-volume mixed liquid dipping sterilization in ten minutes of diluting 800 times;
Step 3, cuttage: hold cutting medium with the seedling-raising box of clean, upper transparent or other closed containers in greenhouse, by cuttings oblique cutting or be laid in cutting medium surface, vinyl cover or film on cuttage bonnet, cover, make regular check on and spray the suitable humidity of clean water maintenance; Step 4, upper basin: will root system be formed and basin in the cuttings growing seedling, and carry out normal cultivation management.In step 2: with the knife blade of sterilization, blade is cut into the size that square cuttings is of a size of 0.5-2 centimeter square.In step 3: cutting medium can with pure perlite, pure vermiculite, perlite and vermiculite mixture, river sand or other common cultivation matrixes, make cutting medium with pure perlite, pure vermiculite, perlite and vermiculite mixture, river sand not few containing germ in organic principle, matrix because of it, thus contribute to improving cuttings motility rate and reproduction rate.In step 3: under the air humidity that cuttage environment will keep more than 80% and light scattering environment, make regular check on water spray, keep matrix moistening, and keep cuttage environmental temperature 20-30 DEG C.
The present invention's leaf that follows conventional lines inserts propagation technique, but has innovation in cuttings size, and substantially increases reproduction coefficient: cuttage respectively forms motility rate and is up to 91.66%, and minimum is 75%, and average survival is 81.96%.Under normal circumstances a length of a film 15-17 ㎝ mature leaf can propagating plantlets 120(calculate by 0.5 × 0.5 cm cuttings specification and 81.96% survival rate) about, be 10 times of normal cutting propagation reproduction coefficient; Within cuttings 25-30 days, take root, within 35-40 days, differentiate bud, within 60-70 days, can basin be transplanted; Without significant difference, (each group of cuttings methyl α-naphthyl acetate of 2,4,10 ㎝ specifications, indolebutyric acid 50 mg/L, clear water soak base portion one minute three kinds and process with not processing control group survival rate in growth regulator process; 0.5 × 0.5 ㎝ blade cuttings is too little, does not do growth regulator process).Under normal circumstances the cuttage of begonia blade use whole lamina or area larger leaf section be cuttings, reproduction coefficient is not high.This technology in the Suitable ranges of 20-30 DEG C, by the cuttings of 0.5 × 0.5 centimetre of size, still can normal differentiation development seedling, seedling speed is the same with the cuttage of routine large cuttings.The method not only breaches the cuttings size limit that people's conventional thought is assert, also substantially increase reproduction coefficient, possesses the high efficiency of method for tissue culture, and overall process does not all require gnotobasis, being the improvement and bring new ideas of vegetative propagation technique, is also the report of research first of relevant endangered species " begonia in spring " blade cottage propagation.This method can be applicable to Begonia and other can insert the floristics of breeding by leaf.
Claims (4)
1. the micro-slotting propagation technique of blade of rare or endangered species " begonia in spring ", is characterized in that, comprise the following steps:
Step one, choose begonia in ripe disease-free spring (
begonia coptidifolia) blade make cuttings material;
Step 2, is cut into square cuttings with the knife blade of sterilization by blade, and with 70% thiophanate methyl wetting powder of dilution 800 times and the 50% carbendazol wettable powder equal-volume mixed liquid dipping sterilization in ten minutes of diluting 800 times;
Step 3, cuttage: hold cutting medium with the seedling-raising box of clean, upper transparent or other closed containers in greenhouse, by cuttings oblique cutting or be laid in cutting medium surface, vinyl cover or film on cuttage bonnet, makes regular check on and sprays clean water and keep suitable humidity;
Step 4, upper basin: will root system be formed and basin in the cuttings growing seedling, and carry out normal cultivation management.
2. the micro-slotting propagation technique of blade of a kind of Precious, Rare, Endangered Begonia plant " begonia in spring " according to claim 1, it is characterized in that, in step 2: with the knife blade of sterilization, blade is cut into the size that square cuttings is of a size of 0.5-2 centimeter square.
3. the micro-slotting propagation technique of blade of a kind of rare or endangered species according to claim 1 " begonia in spring ", it is characterized in that, in step 3: cutting medium can with pure perlite, pure vermiculite, perlite and vermiculite mixture, river sand or other common cultivation matrixes, make cutting medium with pure perlite, pure vermiculite, perlite and vermiculite mixture, river sand not few containing germ in organic principle, matrix because of it, thus contribute to improving cuttings motility rate and reproduction rate.
4. the micro-slotting propagation technique of blade of a kind of rare or endangered species according to claim 1 " begonia in spring ", it is characterized in that, in step 3: under the air humidity that cuttage environment will keep more than 80% and light scattering environment, make regular check on water spray, keep matrix moistening, and keep cuttage environmental temperature 20-30 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310582499.7A CN104642073A (en) | 2013-11-20 | 2013-11-20 | Leaf micro-cuttage propagation technology for rare and endangered plant Begonia coptidifolia |
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| CN201310582499.7A CN104642073A (en) | 2013-11-20 | 2013-11-20 | Leaf micro-cuttage propagation technology for rare and endangered plant Begonia coptidifolia |
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| CN201310582499.7A Pending CN104642073A (en) | 2013-11-20 | 2013-11-20 | Leaf micro-cuttage propagation technology for rare and endangered plant Begonia coptidifolia |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904603A (en) * | 2015-07-02 | 2015-09-16 | 中国科学院华南植物园 | Spring begonia tissue culture breeding method |
| CN105230455A (en) * | 2015-10-11 | 2016-01-13 | 云南省农业科学院花卉研究所 | Method for cultivating begonia fangii using water |
| CN106332626A (en) * | 2016-08-19 | 2017-01-18 | 广西鑫雅皇庭园林工程有限责任公司 | Potting method of begonia |
| CN107318623A (en) * | 2017-08-04 | 2017-11-07 | 贵州省林业科学研究院 | A kind of method of Begonia masoniana cutting propagation |
| CN109548502A (en) * | 2018-12-14 | 2019-04-02 | 浙江中医药大学 | A kind of slotting fast numerous method of radix tetrastigme leaf |
| CN115486363A (en) * | 2022-11-02 | 2022-12-20 | 岭南师范学院 | Seedling raising method for acanthus ilicifolius leaves in water culture |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940151A (en) * | 2010-10-12 | 2011-01-12 | 江苏省中国科学院植物研究所 | New leaf cuttage method for Begonia rex |
| WO2013054272A2 (en) * | 2011-10-11 | 2013-04-18 | Universidad Nacional De Quilmes | Method for controlling leaf-cutting ants |
-
2013
- 2013-11-20 CN CN201310582499.7A patent/CN104642073A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101940151A (en) * | 2010-10-12 | 2011-01-12 | 江苏省中国科学院植物研究所 | New leaf cuttage method for Begonia rex |
| WO2013054272A2 (en) * | 2011-10-11 | 2013-04-18 | Universidad Nacional De Quilmes | Method for controlling leaf-cutting ants |
Non-Patent Citations (3)
| Title |
|---|
| 杜启兰: "丽格秋海棠的繁殖与管理技术", 《林业实用技术》 * |
| 远凌威等: "丽格海棠的组织培养与快速繁殖研究", 《信阳师范学院学报》 * |
| 黄波: "丽格海棠栽培技术详解", 《农村实用工程技术》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904603A (en) * | 2015-07-02 | 2015-09-16 | 中国科学院华南植物园 | Spring begonia tissue culture breeding method |
| CN104904603B (en) * | 2015-07-02 | 2017-05-17 | 中国科学院华南植物园 | Spring begonia tissue culture breeding method |
| CN105230455A (en) * | 2015-10-11 | 2016-01-13 | 云南省农业科学院花卉研究所 | Method for cultivating begonia fangii using water |
| CN105230455B (en) * | 2015-10-11 | 2018-05-04 | 云南省农业科学院花卉研究所 | Fang Shi begonia water planting propagation methods |
| CN106332626A (en) * | 2016-08-19 | 2017-01-18 | 广西鑫雅皇庭园林工程有限责任公司 | Potting method of begonia |
| CN107318623A (en) * | 2017-08-04 | 2017-11-07 | 贵州省林业科学研究院 | A kind of method of Begonia masoniana cutting propagation |
| CN109548502A (en) * | 2018-12-14 | 2019-04-02 | 浙江中医药大学 | A kind of slotting fast numerous method of radix tetrastigme leaf |
| CN115486363A (en) * | 2022-11-02 | 2022-12-20 | 岭南师范学院 | Seedling raising method for acanthus ilicifolius leaves in water culture |
| CN115486363B (en) * | 2022-11-02 | 2024-03-26 | 岭南师范学院 | Seedling raising method for water planting of acanthus praecox leaves |
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Application publication date: 20150527 |
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