CN109548502A - A kind of slotting fast numerous method of radix tetrastigme leaf - Google Patents
A kind of slotting fast numerous method of radix tetrastigme leaf Download PDFInfo
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- CN109548502A CN109548502A CN201811532118.3A CN201811532118A CN109548502A CN 109548502 A CN109548502 A CN 109548502A CN 201811532118 A CN201811532118 A CN 201811532118A CN 109548502 A CN109548502 A CN 109548502A
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- China
- Prior art keywords
- radix tetrastigme
- blade
- cuttage
- fast numerous
- sunshade
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
Abstract
The invention discloses a kind of radix tetrastigme leaves to insert fast numerous method, the petiole of radix tetrastigme blade after disinfection is entirely insertable in the slotting machine matrix of cutting bed, blade patch ground, temperature is maintained at 20~25 DEG C when cuttage, air humidity is controlled 85~95%, with the crape net sunshade of sunshade rate 70%；To spraying water 2-3 times daily during taking root after cuttage, temperature is maintained at 20~25 DEG C, and guarantee air humidity is 75%-90%, and 1-2 days water sprays are primary after taking root, with the crape net sunshade of sunshade rate 70%；After the cuttage 2-3 month, the plant shoots grown up to are transplanted in crop field, normal field management is then carried out.Propagation method is inserted using leaf of the invention, since radix tetrastigme there are a large amount of blades, the quick large-scale production of radix tetrastigme can be realized in a short time, reduce the production cost of radix tetrastigme nursery.
Description
(1) technical field
The present invention relates to a kind of leaves of radix tetrastigme to insert propagation method.
(2) background technique
Radix tetrastigme, scientific name: tetratigma hemsleyanum (Tetrastigma hemsleyanum Diels et Gilg), You Mingjin
Line hoist, hemsley rockvine root, the flat rattan of three leaves, hemsley rockvine root, Shi Baozi, tetrastigma hemsleyanum etc. belong to Vitaceae liana, are Chinese special
Some valuable ingredients of traditional Chinese medicine, are distributed mainly on that Zhejiang, Guizhou, Yunnan and Taiwan etc. are regional, and formulation application is extensive.Radix tetrastigme is as the people
Between common Chinese herbal medicine, possess the good reputations such as " king of medicine ", " anticancer god grass ", with clearing heat and detoxicating, dispelling pathogenic wind and eliminating phlegm, promoting blood circulation and stopping pain function
Effect.Underground root tuber or herb can be used as medicine, and clinical application is extensive, to all kinds of cancers (breast cancer, lung cancer, gastric cancer, liver cancer etc.), heat
Disease (high fever convulsions, pathologic high fever), oedema (inflammatory edema, snakebite) have peculiar curative effect.Modern pharmacology research also proves
Radix tetrastigme has the multiple pharmacological effects such as antipyretic, anti-inflammatory, antitumor, antiviral, liver protection, immunological regulation, has preferable exploitation
Utilization prospects.
Wild radix tetrastigme growing environment requires height, is distributed only over the in the shade face of remote, thickly forested mountains and mountain forest, scattering is arranged at top
There is streams on light, side, and ground is moist.Variation due to environment and mankind's milli it is immoderate it is mad adopt indiscriminate digging, wild resource gradually disappears
It loses, is no longer satisfied the demand in market.Traditional radix tetrastigme requires growing environment high, artificial a large amount of cultivation poor qualities,
And picking is inconvenient, medicinal effects are also undesirable, and demand of the existing market to radix tetrastigme is very big, and how much direct passes of nursery amount
It is to apparent availability.
The existing propagation method of radix tetrastigme is reported in the literature seminal propagation, and cutting propagation, three kinds of Plant Tissue Breeding numerous
Grow mode.But radix tetrastigme seed is few and the speed of growth is slow, at this stage mainly using tissue cultures and cutting propagation to radix tetrastigme into
Row quickly breeding.The technology of radix tetrastigme tissue cultures reaches its maturity, and the propagation in scale of radix tetrastigme seedling may be implemented substantially, but
Cost is also high, and somatic variation frequency increases, and is unsuitable for multiple squamous subculture.Instantly the traditional artificial cultivation of radix tetrastigme is more
For cutting propagation, there is 2-3 stem segment that can be used for cuttage, research shows that radix tetrastigme cuttage survival rate can be by genetic background and the external world
Various influences such as treatment conditions, but radix tetrastigme explant used needs a large amount of radix tetrastigme branch, and breeding amount receives influence.
Radix tetrastigme leaf inserts propagation method, due to radix tetrastigme there are a large amount of blades, it can be achieved that radix tetrastigme plant it is extensive fast
Speed expands numerous, seedling plant type rounding and maternal plant uniform quality.Leaf is inserted than tissue cultures than at low cost, has breeding speed with cuttage ratio
The advantages that degree is fast, amount is big, at low cost, can fast implement the sustainable supply of radix tetrastigme resource.
(3) summary of the invention
It is an object of the present invention to provide a kind of fast breeding methods of radix tetrastigme blade seedling, it can quickly breed largely
It is suitble to the excellent radix tetrastigme seedling of transplanting, meets production needs.
The technical solution adopted by the present invention is that:
The present invention provides a kind of slotting fast numerous method of radix tetrastigme leaf, and the method carries out as follows:
(1) acquisition and pretreatment of blade:
Make in the mature radix tetrastigme blade with petiole of spring or summer and autumn, clip normal growth 1 year or more no disease and pests harm
For cuttings；By the blade after disinfection in 1.0-1.5mgL-1Soaking at room temperature 30min in NAA (α-naphthylacetic acid) aqueous solution, in cool place
Place is dried, and obtains pretreated blade, spare；
(2) cutting bed prepares:
Faintly acid sand is selected, is leveled land, seedbed is built, ditching between seedbed is laid with slotting machine base on seedbed surface
Matter, it is stand-by as cutting bed after sprinkling profoundly water；The slotting machine matrix is 3~5:1 of weight ratio~3:2~4 vegetable garden soil: peat: leech
Stone composition；The vegetable garden soil is common cultivation soil, and fertility is higher, and crumb structure is good, and the peat is turf or mud coal, nutrition
Abundant, venting capability is good, and the vermiculite is natural, inorganic minerals, size 3-6mm, has a chesson, good permeability,
The features such as water absorbing force is strong, and temperature change is small；
(3) cuttage:
The petiole of step (1) pretreatment rear blade is entirely insertable in the slotting machine matrix of step (2) cutting bed, blade patch
Ground, temperature is maintained at 20~25 DEG C when cuttage, and air humidity is controlled 85~95%, with the crape net sunshade of sunshade rate 70%；
(4) it is managed after cuttage:
To spraying water 2-3 times daily during taking root after cuttage, temperature is maintained at 20~25 DEG C, and guarantee air humidity is 75%-
90%, 1-2 days water sprays are primary after taking root, with the crape net sunshade of sunshade rate 70%；
(5) management in later period is transplanted:
After the cuttage 2-3 month, the plant shoots grown up to are transplanted in crop field, normal field management is then carried out.
Further, the long 5-12cm of blade, the long 3-5cm of petiole in step (1) as cuttings.
Further, step (1) disinfection is that (being purchased from Sichuan state light agrochemical share has with 25% carbendazol wettable powder
Limit company) 400 times of v aqueous solutions impregnate blade 30min and carry out disinfection.
Further, the width in step (2) described seedbed is 1.2m, is highly 0.15m.The furrow width of ditching between the seedbed
30cm, depth 20cm.
Further, step (2) faintly acid sand pH6.2~6.7.
Further, 10~15cm of thickness of step (2) the slotting machine matrix.
Further, step (2) the slotting machine matrix is the vegetable garden soil of weight ratio 4:2:3: peat: vermiculite composition.
Compared with prior art, beneficial effect of the present invention is mainly reflected in:
(1) propagation method is inserted using leaf of the invention, it, can be real in a short time since radix tetrastigme has a large amount of blades
The existing quick large-scale production of radix tetrastigme, reduces the production cost of radix tetrastigme nursery；
(2) same radix tetrastigme maternal plant product can be produced due to being the mode of nourishing and generating by inserting propagation method using leaf of the invention
The consistent radix tetrastigme plant of matter.
(4) specific embodiment
The present invention is described further combined with specific embodiments below, but protection scope of the present invention is not limited in
This:
1 radix tetrastigme leaf of embodiment inserts quick-breeding method
1) acquisition and processing of blade
In spring (the 4-5 month) or autumn (the 9-11 month), clip normal growth 1 year or more no disease and pests harm with petiole
Mature radix tetrastigme blade is as cuttings, the long 5-12cm of blade, the long 3-5cm of petiole；(four are purchased from 25% carbendazol wettable powder
Chuan Guoguang agrochemical limited liability company) 400 times of v aqueous solutions impregnate blade 30min and carry out disinfection, then the blade that will be disinfected
Using 1.5mgL-1NAA aqueous solution soaking 30min, dries in the air surface moisture in shady place, and as pretreated blade is spare.
Same radix tetrastigme blade is selected, is impregnated without carbendazim, leaf is done without NAA aqueous solution soaking and inserts control；
2) cutting bed prepares
Faintly acid (pH6.2~6.7) sand is selected, is leveled land, building seedbed, (width 1.2m is highly
0.15m).Ditching between seedbed, furrow width 30cm, depth 20cm.The slotting machine matrix of 10~15cm thickness, slotting machine are laid on seedbed surface
The weight allocation ratio of matrix is vegetable garden soil: peat: vermiculite=4:2:3, stand-by as cutting bed after sprinkling profoundly water；The vegetable garden soil
For common cultivation soil, fertility is higher, and crumb structure is good, and the peat is turf or mud coal, and full of nutrition, venting capability is good,
The vermiculite is natural, inorganic minerals, and size 3-6mm has chesson, and good permeability, water absorbing force is strong, temperature change
The features such as small；
3) cuttage
Using entire leaf cutting, the pretreated petiole of step 1) is entirely insertable to the slotting machine matrix of step 2) cutting bed, blade
Patch ground.Squirt slotting machine matrix with water when cuttage, temperature is maintained at 20~25 DEG C when cuttage, air humidity control 85~95%,
With the crape net sunshade of sunshade rate 70%.Radix tetrastigme blade and blade inserts control and uses same method cuttage；
4) it is managed after cuttage
To spraying water 2-3 times daily during taking root after cuttage, temperature is maintained at 20~25 DEG C, and guarantee air humidity is 75%-
90%, 1-2 days water sprays are primary after taking root, with the crape net sunshade of sunshade rate 70%.Radix tetrastigme blade and blade inserts control using same
Method management；
5) management in later period is transplanted
After the cuttage 2-3 month, the radix tetrastigme blade survival rate of carbendazim and NAA processing reaches 90%, and well developed root system, sprouting sends out
Out；And leaf inserts control group survival rate 15%, root system is few, has no that sprouting issues.The radix tetrastigme leaf transplanting strain seedling replanting that will grow up to
In crop field, normal field management is then carried out.
Finally it is pointed out that above example is only the more representational example of the present invention.Obviously, technical side of the invention
Case is not limited to examples detailed above, can also be there are many deformation, and those skilled in the art can be from present disclosure
All deformations for directly exporting or associating, are considered as protection scope of the present invention.
Claims (7)
1. a kind of radix tetrastigme leaf inserts fast numerous method, it is characterised in that the method carries out as follows:
(1) acquisition and pretreatment of blade:
In spring or summer and autumn, the mature radix tetrastigme blade with petiole of clip normal growth 1 year or more no disease and pests harm is as slotting
Fringe；By the blade 1.0-1.5mgL after disinfection-1NAA aqueous solution soaking 30min, dries in shady place, after being pre-processed
Blade；
(2) cutting bed prepares:
Selection sand containing faintly acid, which is leveled land, builds seedbed, ditching between seedbed；It is laid with slotting machine matrix on seedbed surface, is poured
Cutting bed is used as after permeable, for use；The slotting machine matrix is 3~5:1 of weight ratio~3:2~4 vegetable garden soil: peat: vermiculite group
At；
(3) cuttage:
The petiole of step (1) pretreatment rear blade is entirely insertable in step (2) slotting machine matrix, blade patch ground, temperature when cuttage
20~25 DEG C are maintained at, air humidity is controlled 85~95%, with the crape net sunshade of sunshade rate 70%；
(4) it is managed after cuttage:
To spraying water 2-3 times daily during taking root after cuttage, temperature is maintained at 20~25 DEG C, and guarantee air humidity is 75%-90%,
1-2 days water sprays are primary after taking root, with the crape net sunshade of sunshade rate 70%；
(5) management in later period is transplanted:
After the cuttage 2-3 month, the plant shoots grown up to are transplanted in crop field, normal field management is then carried out.
2. radix tetrastigme leaf as described in claim 1 inserts fast numerous method, it is characterised in that the long 5- of blade of step (1) described cuttings
12cm, the long 3-5cm of petiole.
3. radix tetrastigme leaf as described in claim 1 inserts fast numerous method, it is characterised in that step (1) described sterilization method: using
25% 400 times of carbendazol wettable powder v aqueous solution impregnates blade 30min and carries out disinfection.
4. radix tetrastigme leaf as described in claim 1 inserts fast numerous method, it is characterised in that sandy soil containing faintly acid described in step (2)
Earth pH6.2~6.7.
5. radix tetrastigme leaf as described in claim 1 inserts fast numerous method, it is characterised in that step (2) slotting machine matrix laying depth 10
~15cm.
6. radix tetrastigme leaf as described in claim 1 inserts fast numerous method, it is characterised in that the width in step (2) described seedbed is
1.2m is highly 0.15m.The furrow width 30cm of ditching between the seedbed, depth 20cm.
7. radix tetrastigme leaf as described in claim 1 inserts fast numerous method, it is characterised in that step (2) the slotting machine matrix is weight
Vegetable garden soil than 4:2:3: peat: vermiculite composition.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811532118.3A CN109548502A (en) | 2018-12-14 | 2018-12-14 | A kind of slotting fast numerous method of radix tetrastigme leaf |
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|---|---|---|---|
| CN201811532118.3A CN109548502A (en) | 2018-12-14 | 2018-12-14 | A kind of slotting fast numerous method of radix tetrastigme leaf |
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| CN109548502A true CN109548502A (en) | 2019-04-02 |
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Citations (9)
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|---|---|---|---|---|
| CN102144484A (en) * | 2011-01-11 | 2011-08-10 | 江苏九久环境科技有限公司 | Cutting propagation method of Photinia lochengensis |
| CN104521714A (en) * | 2015-01-07 | 2015-04-22 | 张红 | Leaf cuttage rapid propagation method of jonquil |
| CN104642073A (en) * | 2013-11-20 | 2015-05-27 | 上海辰山植物园 | Leaf micro-cuttage propagation technology for rare and endangered plant Begonia coptidifolia |
| CN104782416A (en) * | 2015-03-30 | 2015-07-22 | 浙江省林业科学研究院 | Cultivation medium special for radix tetrastigme seedling |
| CN105494092A (en) * | 2015-12-08 | 2016-04-20 | 杭州市农业科学研究院 | Induction and propagating method of Tetrastigma hemsleyanum Diels et Gilg adventitious root |
| CN107624397A (en) * | 2017-10-20 | 2018-01-26 | 百色学院 | A kind of cuttage breeding method of Pasania cuspidata |
| CN108012683A (en) * | 2017-11-29 | 2018-05-11 | 广州市新茂生态农业发展有限公司 | A kind of red autumnal leaves podocarpus blade cuttage breeding method |
| CN108323423A (en) * | 2017-08-14 | 2018-07-27 | 温州科技职业学院 | The water cutting propagation method of radix tetrastigme |
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2018
- 2018-12-14 CN CN201811532118.3A patent/CN109548502A/en active Pending
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| CN104642073A (en) * | 2013-11-20 | 2015-05-27 | 上海辰山植物园 | Leaf micro-cuttage propagation technology for rare and endangered plant Begonia coptidifolia |
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| CN105494092A (en) * | 2015-12-08 | 2016-04-20 | 杭州市农业科学研究院 | Induction and propagating method of Tetrastigma hemsleyanum Diels et Gilg adventitious root |
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| CN108012683A (en) * | 2017-11-29 | 2018-05-11 | 广州市新茂生态农业发展有限公司 | A kind of red autumnal leaves podocarpus blade cuttage breeding method |
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Application publication date: 20190402 |