CN104521714A - Leaf cuttage rapid propagation method of jonquil - Google Patents
Leaf cuttage rapid propagation method of jonquil Download PDFInfo
- Publication number
- CN104521714A CN104521714A CN201510005454.2A CN201510005454A CN104521714A CN 104521714 A CN104521714 A CN 104521714A CN 201510005454 A CN201510005454 A CN 201510005454A CN 104521714 A CN104521714 A CN 104521714A
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- cuttage
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- jonquil
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 235000013862 Narcissus jonquilla Nutrition 0.000 title claims abstract description 18
- 244000223072 Narcissus jonquilla Species 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 claims abstract description 12
- 239000011159 matrix material Substances 0.000 claims description 21
- 241001091572 Kalanchoe Species 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 239000012531 culture fluid Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 239000007921 spray Substances 0.000 claims description 6
- 230000012010 growth Effects 0.000 claims description 5
- 241000195940 Bryophyta Species 0.000 claims description 4
- 238000005520 cutting process Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000003415 peat Substances 0.000 claims description 4
- 239000010451 perlite Substances 0.000 claims description 4
- 235000019362 perlite Nutrition 0.000 claims description 4
- 241000894007 species Species 0.000 claims description 4
- 239000010455 vermiculite Substances 0.000 claims description 4
- 235000019354 vermiculite Nutrition 0.000 claims description 4
- 229910052902 vermiculite Inorganic materials 0.000 claims description 4
- 241001232787 Epiphragma Species 0.000 claims description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 230000003020 moisturizing effect Effects 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
- 238000003892 spreading Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 5
- 241000196324 Embryophyta Species 0.000 description 6
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 description 5
- 239000012452 mother liquor Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- VGKONPUVOVVNSU-UHFFFAOYSA-N naphthalen-1-yl acetate Chemical compound C1=CC=C2C(OC(=O)C)=CC=CC2=C1 VGKONPUVOVVNSU-UHFFFAOYSA-N 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000319930 Bryophyllum <angiosperm> Species 0.000 description 1
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- NNBFNNNWANBMTI-UHFFFAOYSA-M brilliant green Chemical compound OS([O-])(=O)=O.C1=CC(N(CC)CC)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](CC)CC)C=C1 NNBFNNNWANBMTI-UHFFFAOYSA-M 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a leaf cuttage rapid propagation method of jonquil. The method comprises the content of leaf cuttage, medium cuttage, preparation of cuttage containers, preparation of culture solutions, a cuttage method, culture conditions, management after cuttage and the like. According to the cuttage method, the sprouting and root growing speed of the leaves of the jonquil is extremely high, the problem that the sprouting speed in leaf cuttage of the jonquil is especially low in production is solved, the seedling culture period is shortened greatly, and the survival rate is improved. The leaf cuttage rapid propagation method is easy to operate, special instruments and devices are not needed, and the method is suitable for household small-batch seedling culture and suitable for large-scale factory production.
Description
Technical field
The invention belongs to the propagation technique field of plant, especially relate to a kind of method of Kalanchoe blossfediana Leaves cottage propagation.
Background technology
Jonquil (
kalanchoe blossfel.diana T.) be the perennial fleshy-juicy plant of Crassulaceae Bryophyllum, its plant type is compact, the emerald green succulence of blade, and flower is intensive, pattern enriches, and the florescence is long, can continue 3-5 month, full-bloom stage is on the occasion of around the Spring Festival, and both considerable leaves, can admire the beauty of flowers again, and strong stress resistance, extensive management, be a kind of extensively popular winter-spring season indoor appreciation flowers, jonquil also can all herbal medicine, has the effects such as heat-clearing, removing toxic substances, hemostasis, development prospect is wide, occupies an important position in pot flowers at home and abroad.
At present, the breeding of jonquil mainly adopts cuttage and tissue-culturing rapid propagation etc.Although tissue-culturing rapid propagation reproduction coefficient is higher, program is numerous and diverse, drops into high, and require special instrument and equipment, technical requirement is high; Although cottage propagation reproduction coefficient is relatively low, due to simple to operate, technical requirement is low, less investment, instant effect, production on apply more; The cuttage of jonquil comprises again stem segment cuttage and blade cuttage, and stem segment cuttage easily survives, but often destroys comparatively large to kind of strain, and stem section limited amount; And blade cuttage is little to kind of strain destructiveness, cuttage material more easily obtains.Research according to bibliographical information and the author finds, although traditional Kalanchoe blossfediana Leaves cuttage is taken root easily, and difficulty of sprouting (Li Yingqi, the cottage propagation of jonquil and management, flower, bird, insect, and fish, 2009,9:25-26; Wu Xinqin, jonquil whole year production raising technology, Shanxi Forestry science and technology, 2008,9 (3): 40-42; Wang Yun, also says that leaf inserts jonquil, Chinese flower report, 2003,10:1), seriously limit the efficiency of Kalanchoe blossfediana Leaves cottage propagation, therefore, be necessary the method that research Kalanchoe blossfediana Leaves cuttage stratification is taken root.
Summary of the invention
The object of the invention is: produce Leaf cuttage for jonquil and to sprout difficulty, the bottleneck problems such as seedling is slow, a kind of Kalanchoe blossfediana Leaves cuttage root-taking is provided to germinate rapidly, root amount is enriched, bud is sprouted early, the method for the blade cuttage of robust growth, for the blade cottage propagation of jonquil provides technological guidance.
In order to solve the problem, the invention provides following technical solution
The present invention is using simple, practical, efficient as guideline.The plant growth regulator such as the basic element of cell division, growth hormone based on debita spissitudo can promote the principle of plant sprout differentiation and root growth.Mix as cutting medium using the peat composed of rotten mosses, perlite, vermiculite in the ratio of 1:1:1, with NAA0.05-0.15mg/l+IBA0.05-0.15mg/l as cuttage culture fluid, the middle and lower part blade getting robust growth in the strain of jonquil good species is cuttage material, be equipped with the condition of culture such as suitable temperature, humidity, the Rapid Rooting achieving Kalanchoe blossfediana Leaves cuttage germinates, and substantially increases production efficiency.
A method for Kalanchoe blossfediana Leaves cuttage and quick-propagation, comprises the following steps.
The preparation of A, cuttage blade: the middle and lower part blade choosing robust growth in the strain of jonquil good species, breaks gently from petiole base, splays a kerf in the petiole base back side, is put into frescade and dries in the air 12-24hr.
The preparation of B, cutting medium: the not used peat composed of rotten mosses, perlite, vermiculite, mix in the ratio of 1:1:1, sterilizes with the sunlight exposure of wanting crossed.
C, cuttage vessel: can gather materials on the spot, shallow basin, bubble chamber, seedling dish can, large batch ofly also can to carry out at seedling bed.
The formula of D, culture fluid: NAA0.05-0.15mg/l+IBA0.05-0.15mg/l, PH:5.8-6.2.
E, cottage method: the matrix spreading 4-8cm in cuttage vessel or on seedling bed, irrigates matrix with culture fluid, then lie in Kalanchoe blossfediana Leaves in matrix, press with have gentle hands, whole blade is all embedded in matrix, and especially petiole base fully will contact with matrix, then epiphragma moisturizing.
F, condition of culture: light culture and dark culture hockets, light is cultivated: temperature 20-25 DEG C, illumination 1000-2000lux, time 12-16hr; Light culture: temperature 16-20 DEG C, time 8-12hr; Namely after cuttage about about 10 days start to root, and survival rate is more than 95%.
Manage after G, cuttage: after cuttage first 10 days, every 2 days water sprays once, make matrix remain moisture state, air humidity remains on 70%-90%.After 10 days, film is removed, adding mass percent concentration with the urea that mass percent concentration is 0.1%-0.5% is that the potassium dihydrogen phosphate of 0.1%-0.5% carries out blade fertilising once, reduce water spray number of times gradually, make matrix see dry see wet, after one month, seedling approximately grow to two leaves wholeheartedly time, can can cut separately pot transplanting on seedling together with blade, also, enter normal rich water quality management.
The major advantage that the method for Kalanchoe blossfediana Leaves cuttage and quick-propagation stated by institute of the present invention book is:
Adopt cottage method provided by the invention, the rudiment rooting rate of Kalanchoe blossfediana Leaves is exceedingly fast, solve the difficult problem that Kalanchoe blossfediana Leaves cuttage rudiment is slow especially, substantially reduce growing-seedling period, improve survival rate, by method provided by the invention, Kalanchoe blossfediana Leaves can be made germination in about 10 days, take root, at least shorten the time of 1 month compared with traditional blade cottage method, survival rate improves 40%-50%.
Reproduction coefficient is higher, and blade cuttage is relative to stem segment cuttage, and can not damage the plant type of kind of strain, material is easy to get, and the expansion especially for the less appreciable variety of individual plant is numerous outstanding aobvious important.
Method is simple, cost is low.Cottage method provided by the invention is simple to operate, does not need special instrument and equipment, has both been applicable to family's small lot nursery, has also been applicable to large-scale factorial praluction.
Accompanying drawing explanation
The Kalanchoe blossfediana Leaves rooted during Fig. 1 cuttage 10 days.
The upgrowth situation of bud during Fig. 2 cuttage 1 month.
Below in conjunction with example, the invention will be further described.
Embodiment
A method for Kalanchoe blossfediana Leaves cuttage and quick-propagation, comprises the following steps.
The preparation of A, cuttage blade: the middle and lower part blade choosing robust growth in the strain of jonquil good species, breaks gently from petiole base, splays a kerf in the petiole base back side, and blade back is put into frescade upward and dries in the air 12-24hr.
The preparation of B, cutting medium: the not used peat composed of rotten mosses, perlite, vermiculite, mix in the ratio of 1:1:1, stand-by.
C, cuttage vessel: can gather materials on the spot, shallow basin, bubble chamber, seedling dish can, large batch ofly also can to carry out at seedling bed.
The preparation of D, nutrient solution: the preparation of 0.1mg/ml methyl α-naphthyl acetate (NAA) mother liquor: the NAA taking 10mg, with the anhydrous alcohol solution of a small amount of (about 1ml), then is settled to 100ml with distilled water.
The preparation of 0.1mg/ml indolebutyric acid (IBA) mother liquor: the IBA taking 10mg, with the anhydrous alcohol solution of a small amount of (about 1ml), then is settled to 100ml with distilled water.
The preparation of nutrient solution: get methyl α-naphthyl acetate (NAA) the mother liquor 0.5-1.5ml of 0.1mg/ml, indolebutyric acid (IBA) mother liquor 0.5-1.5ml respectively, be diluted to 1000ml, existing cuttage nutrient solution.
E, cottage method
Spread the matrix of 4-8cm in cuttage vessel or on seedling bed, with culture fluid, matrix is irrigated, then Kalanchoe blossfediana Leaves is lain in matrix, press with have gentle hands, whole blade is all embedded in matrix, and especially petiole base fully will contact with matrix, then epiphragma moisturizing.
F, condition of culture: light culture and dark culture hockets, light is cultivated: temperature 20-25 DEG C, illumination 1000-2000lux, time 12-16hr; Light culture: temperature 16-20 DEG C, time 8-12hr; Namely after cuttage about about 10 days start to root, and survival rate is more than 95%.
Manage after G, cuttage: after cuttage first 10 days, every 2 days water sprays once, make matrix remain moisture state, air humidity remains on 70%-90%.After 10 days, film is removed, adding mass percent concentration with the urea that mass percent concentration is 0.1%-0.5% is that the potassium dihydrogen phosphate of 0.1%-0.5% carries out blade fertilising once, reduce water spray number of times gradually, make matrix see dry see wet, after one month, seedling approximately grow to two leaves wholeheartedly time, can can cut separately pot transplanting on seedling together with blade, also, enter normal rich water quality management.
The present invention is not limited to preferred forms described above, and the identical close method of any any jonquil kind drawn under the present invention's enlightenment, all drops within protection scope of the present invention.
Claims (1)
1. a method for Kalanchoe blossfediana Leaves cuttage and quick-propagation, is characterized in that, comprises the following steps:
The preparation of A, cuttage blade: the middle and lower part blade choosing robust growth in the strain of jonquil good species, breaks gently from petiole base, splays a kerf in the petiole base back side, and blade back is put into frescade upward and dries in the air 12-24hr;
b,the preparation of cutting medium: the not used peat composed of rotten mosses, perlite, vermiculite, mix in the ratio of 1:1:1, sterilizes with the sunlight exposure of wanting crossed;
c,cuttage vessel: can gather materials on the spot, shallow basin, bubble chamber, seedling dish can, large batch ofly also can to carry out at seedling bed;
d,the formula of culture fluid: NAA0.05-0.15mg/l+IBA0.05-0.15mg/l, PH:5.8-6.2;
e,cottage method: the matrix spreading 4-8cm in cuttage vessel or on seedling bed, irrigates matrix with culture fluid, then lies in Kalanchoe blossfediana Leaves in matrix, press with have gentle hands, whole blade is all embedded in matrix, and especially petiole base fully will contact with matrix, then epiphragma moisturizing;
f,condition of culture: light culture and dark culture hockets, light is cultivated: temperature 20-25 DEG C, illumination 1000-2000lux, time 12-16hr; Light culture: temperature 16-20 DEG C, time 8-12hr;
g,manage after cuttage: after cuttage first 10 days, every 2 days water sprays once, matrix is made to remain moisture state, air humidity remains on 70%-90%, after 10 days, film is removed, adding mass percent concentration with the urea that mass percent concentration is 0.1%-0.5% is that the potassium dihydrogen phosphate of 0.1%-0.5% carries out blade fertilising once, reduce water spray number of times gradually, make matrix see dry see wet, after one month, seedling approximately grow to two leaves wholeheartedly time, can can cut separately pot transplanting on seedling together with blade, also, enter normal rich water quality management.
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| CN201510005454.2A CN104521714B (en) | 2015-01-07 | 2015-01-07 | Leaf cuttage rapid propagation method of jonquil |
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|---|---|---|---|
| CN201510005454.2A CN104521714B (en) | 2015-01-07 | 2015-01-07 | Leaf cuttage rapid propagation method of jonquil |
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| Publication Number | Publication Date |
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| CN104521714A true CN104521714A (en) | 2015-04-22 |
| CN104521714B CN104521714B (en) | 2017-02-22 |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105830736A (en) * | 2016-05-05 | 2016-08-10 | 巢湖市鑫宇良种农民专业合作社 | Method for rapidly cultivating plants in factories through petiole growing points |
| CN106171871A (en) * | 2016-06-13 | 2016-12-07 | 湖北文理学院 | Kalanchoe blossfediana Leaves sand training rooting method |
| CN106234177A (en) * | 2016-06-13 | 2016-12-21 | 湖北文理学院 | Kalanchoe blossfeldiana water planting in winter promotees to bloom method |
| CN106305051A (en) * | 2016-08-16 | 2017-01-11 | 淮北师范大学 | Leaf cutting propagation method of succulent plants of xPachyveria pachytoides |
| CN106718763A (en) * | 2016-12-30 | 2017-05-31 | 山西省农业科学院园艺研究所 | A kind of red-spotted stonecrop single node cutting breeding method |
| CN109496814A (en) * | 2018-12-13 | 2019-03-22 | 福建省中科生物股份有限公司 | A kind of indoor cuttage ciltivating process of jonquil |
| CN109548502A (en) * | 2018-12-14 | 2019-04-02 | 浙江中医药大学 | A kind of slotting fast numerous method of radix tetrastigme leaf |
| CN110754319A (en) * | 2019-11-26 | 2020-02-07 | 四川云辰园林科技有限公司 | Cultivation method of Christmas kalanchoe |
| CN113179774A (en) * | 2021-05-18 | 2021-07-30 | 四川省农业科学院经济作物育种栽培研究所 | Low-altitude rapid breeding method for plateau rhodiola rosea |
| CN114616990A (en) * | 2022-03-29 | 2022-06-14 | 成都大学 | Aconitum carmichaeli cutting breeding method |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105830736A (en) * | 2016-05-05 | 2016-08-10 | 巢湖市鑫宇良种农民专业合作社 | Method for rapidly cultivating plants in factories through petiole growing points |
| CN106171871A (en) * | 2016-06-13 | 2016-12-07 | 湖北文理学院 | Kalanchoe blossfediana Leaves sand training rooting method |
| CN106234177A (en) * | 2016-06-13 | 2016-12-21 | 湖北文理学院 | Kalanchoe blossfeldiana water planting in winter promotees to bloom method |
| CN106305051A (en) * | 2016-08-16 | 2017-01-11 | 淮北师范大学 | Leaf cutting propagation method of succulent plants of xPachyveria pachytoides |
| CN106305051B (en) * | 2016-08-16 | 2019-11-19 | 淮北师范大学 | A kind of slotting propagation method of the leaf of succulent winter beauty |
| CN106718763A (en) * | 2016-12-30 | 2017-05-31 | 山西省农业科学院园艺研究所 | A kind of red-spotted stonecrop single node cutting breeding method |
| CN109496814A (en) * | 2018-12-13 | 2019-03-22 | 福建省中科生物股份有限公司 | A kind of indoor cuttage ciltivating process of jonquil |
| CN109548502A (en) * | 2018-12-14 | 2019-04-02 | 浙江中医药大学 | A kind of slotting fast numerous method of radix tetrastigme leaf |
| CN110754319A (en) * | 2019-11-26 | 2020-02-07 | 四川云辰园林科技有限公司 | Cultivation method of Christmas kalanchoe |
| CN113179774A (en) * | 2021-05-18 | 2021-07-30 | 四川省农业科学院经济作物育种栽培研究所 | Low-altitude rapid breeding method for plateau rhodiola rosea |
| CN113179774B (en) * | 2021-05-18 | 2023-10-24 | 四川省农业科学院经济作物育种栽培研究所 | Low-altitude rapid breeding method for rhodiola sachalinensis |
| CN114616990A (en) * | 2022-03-29 | 2022-06-14 | 成都大学 | Aconitum carmichaeli cutting breeding method |
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| CN104521714B (en) | 2017-02-22 |
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