CN103907497A - Rapid cutting propagation method of test-tube plum plantlets - Google Patents
Rapid cutting propagation method of test-tube plum plantlets Download PDFInfo
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Abstract
A rapid cutting propagation method of test-tube plum plantlets is characterized by including the steps of 1, cultivating test-tube plantlets; 2, selecting and treating cuttings, namely cutting each test-tube plantlet into an upper part and a lower part, making user the lower part is 2-3cm in length, cutting to make V-shaped cuts in the upper portion every 3-4cm with a surgical knife, soaking in hormones different in concentration for 20s, placing the upper part in a flat tray, burying with mixed medium, exposing a top bud only, and completely irrigating; 3, inducing the cuttings to root, namely planting the cuttings on the perlite mixed medium in spring and autumn each year; 4, managing after cuttage. The mixed medium is made with mixture of perlite, vermiculite, peat soil, and river sand according to the ratio of 2:2:1:1.
Description
Technical field
The invention belongs to plant seedling propagation technique field, be specially a kind of method of plum test-tube plantlet cuttage and quick-propagation.
Background technology
Though plum has long history in the cultivation of China, still, its distributed areas are less.In recent years, plum, as the fruit a kind ofly going on the market early, delicious look fresh, more and more caused people's attention.A lot of areas all prepare to introduce a fine variety or expand area under cultivation, are badly in need of a large amount of nursery stocks in production. and slow according to traditional approach breeding plum speed, can not satisfy the demand far away.For this reason, domestic many units carry out the Propagations of Teat Tube Seedlings of plum.
The cuttage and quick-propagation technology of test-tube plantlet is a novel practical technology of taking in recent years.Test-tube plantlet cuttage technique has been obtained good effect in the plant production such as potato, honeysuckle, grape, the cultivation mode of efficient for exploring, thrifty, low consumption, we produce this technology for plum stock, adopt root induction phase group training seedling to carry out the research of test-tube plantlet cuttage technique, obtain nearly ten thousand strains of detoxification plum stock, within the group training seedling excessive transplanting time time of short 20 days, allow tissue cultured test-tube seedling proliferation 3-4 doubly, respond well.
The existing cottage method of plum dwarfing rootstock has two kinds: cuttage, epicormic branch cuttage.Cuttage survival rate is extremely low.Epicormic branch cuttage is that seedling-growing method is cut in employing, selection is different from cuttage, needs hot and humid environment, and survival rate is the highest also only has 70%.The domestic bibliographical information that does not still do the cuttage of plum test-tube plantlet at present.
Summary of the invention
For the problem of above-mentioned existence, the technical problem that the present invention mainly solves is to provide the method for plum test-tube plantlet cuttage and quick-propagation, and for solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
(1) test-tube plantlet is cultivated: first culture is chosen robust growth spring, without the tender young sprout of annual children of damage by disease and insect, with running water flow wash 30～60min, be transferred on superclean bench, with 75% alcohol to young sprout stem with bud sterilization 30～45s, then in 0.1% mercuric chloride, soak 5～11min, aseptic water washing 4～6 times, stem apex part is peeled off to the size to 0.5～1mm, remainder is cut into the stem section of 1cm left and right, be inoculated in respectively on inducing culture, under illumination condition, cultivate, inducing culture is MS+6-BA3.0mg/L+IAA0.5mg/L+ sucrose 30g/L+ agar 10g/L, pH value is 6.5, condition of culture is: intensity of illumination 3000LX, light application time 18h, 26 ℃ of temperature, incubation time 17d, cultivate through induction, can induce axillary bud sprouting, propagation is cultivated: after just for explant seedling differentiation, forward on proliferated culture medium, culture of rootage proceeds to the tender seedling of stalwartness more than about height 1.5cm in root media and cultivates, just culture and proliferated culture medium basal culture medium are MS, culture of rootage minimal medium is 1/2MS, agar 5.0～5.5g/L, sucrose 30g/L, pH value 5.6～.8, intensity of illumination is 1500～2000lx, light application time is 14h/d, cultivation temperature is controlled at (25 ± 1) ℃, relative air humidity is 40%～70%, test-tube seedling transplanting is chosen robust growth and is had the test-tube plantlet of more than 3 1cm left and right Xin Gen to carry out hardening, under natural daylight, tame hardening 1 week, then open bottle cap, spray clear water every day 4～5 times, after 3 days, take out seedling, clean the residual medium being attached on root, transplant in matrix, wherein said grafting matrix is humus soil mixing vermiculite, and mixed proportion is 2: 1, puts into little shed, regularly water spray, imposed one time wash fertilizer, and increases gradually illumination every 1 week, treat that nutrition pot seedling has 4～5 young leaves, when plant height 5cm is above, move into land for growing field crops cuttage,
(2) cuttage is chosen and is processed: test-tube plantlet is intercepted as upper and lower two parts, guarantee that lower partial-length is at 2-3 centimetre, upper part interval 3-4 centimetre is cut out to V-type wound with scalpel, in variable concentrations hormone, soak after 20 seconds, upper part entirety is put into square position, bury with mixed-matrix, only expose top bud, water permeable; Wherein said hormone is: heteroauxin, ABT-1 root-inducing powder, ABT-6 root-inducing powder; Described test-tube plantlet is the GiselaNo.5 of root induction phase or the group training seedling of No. 6; In described variable concentrations hormone, soak and refer to: concentration is 0.4,0.5,0.6mg/L heteroauxin, ABT-1 root-inducing powder, ABT-6 root-inducing powder;
(3) cuttage root induction: in annual spring, Qiu Liangji, cuttage on perlite mixed-matrix; Mixed-matrix is wherein perlite, vermiculite, turfy soil, river sand 2: 2: 1 by weight: 1 ratio is mixed;
(4) management after cuttage: guarantee that temperature of shed is at 26-28 ℃, humidity is at 50-60%, spray every other day carbendazim 1-2 time, after cuttage 5-7 days, spray 1-5 time time insecticide, every minor tick 2-3 days, whether observation in 10-14 days takes root, and executes a urea the last week of emerging to impel the healthy and strong growth of seedling; After 14 days, the new root of cutting begins to take shape, and after 30 days, seedling forms, and now carries out hardening, transplanting; Wherein said carbendazim refers to the aqueous solution of 1250-1670 mg/kg; Time insecticide refers to the aqueous solution of 1250-1670 mg/kg.
Plum dwarfing rootstock of the present invention cuttage and existing difference are:
1, existing hardwood cuttage method output is the common seedling of not detoxification, rooting rate less than 30%, and epicormic branch cuttage is the highest also just only has 70%.The seedling of cuttage output of the present invention is plum detoxic seedling, and rooting rate is up to 85%; And plum detoxic seedling provides a lot of benefits to grower, as few in detoxic seedling virus disease, a lot of hereditary illnesss disappear substantially, have guaranteed the survival rate of nursery stock, and allow nursery stock output improve at least 30%.
2, cottage method of the present invention energy cuttage twice in a year, breeding rate is high, and the time is short.Temperature humidity is had relatively high expectations; Existing method cuttage can only do before the Ching Ming Festival in the Spring Festival, and epicormic branch cuttage can only do in August in summer, and time restriction is stricter.
3, cottage method labor intensive of the present invention is few, and cost is low, and work efficiency is high, within 1 year, can use at spring and autumn; The existing hardwood cuttage method cuttage amount of labour is large, and requires very high to sheared edge, cuttage gimmick.The survival rate of seedling art is relatively low.
The good effect that plum test-tube plantlet cuttage and quick-propagation method disclosed by the invention compared with prior art had is:
(1) plum test-tube plantlet cuttage and quick-propagation method of the present invention, what wherein the cuttage of test-tube plantlet adopted is that the seedling that cultivates of test tube is as material.Because tissue cultured test-tube seedling is very tender seedling, look like the equally tender seedling of bean sprouts, seedling cell water content is at this time large especially, do not form the interior that trees are the same, fragile, general test-tube plantlet whether survive must see behind immigration land for growing field crops its degree of lignification OK, judge that with this it adapts to external environment and life ability, therefore, the seedling that test tube cultivates does not have lignification completely, generally moves into booth transplanting land for growing field crops adaptation later in two months and just starts lignification after half a year.
(2) test-tube plantlet cottage breeding of the present invention out be detoxification plum seedling, experimental data shows: half detoxification plum seedling output is than detoxification plum height of seedling 30% not, full detoxification plum seedling output is than detoxification plum height of seedling 45% not.Therefore, method of the present invention can solve the fast numerous production problem of scale of plum detoxic seedling.
(3) test-tube plantlet cottage breeding of the present invention out be detoxification plum seedling, maximum feature is: can breed in a short time 3-4 detoxic seedling doubly, can effectively solve like this some hereditary illnesss, guarantee the survival rate of nursery stock, more be conducive to large-scale breeding and produce.
Embodiment
Embodiment 1
(1) test-tube plantlet is cultivated: first culture is chosen robust growth spring, without the tender young sprout of annual children of damage by disease and insect, with running water flow wash 30min, be transferred on superclean bench, with 75% alcohol to young sprout stem with bud sterilization 30s, then in 0.1% mercuric chloride, soak 5min, aseptic water washing 4～6 times, stem apex part is peeled off to the size to 0.5mm, remainder is cut into the stem section of 1cm left and right, be inoculated in respectively on inducing culture, under illumination condition, cultivate, inducing culture is MS+6-BA3.0mg/L+IAA0.5mg/L+ sucrose 30g/L+ agar 10g/L, pH value is 6.5, condition of culture is: intensity of illumination 3000LX, light application time 18h, 26 ℃ of temperature, incubation time 17d, cultivate through induction, can induce axillary bud sprouting, propagation is cultivated: after just for explant seedling differentiation, forward on proliferated culture medium, culture of rootage proceeds to the tender seedling of stalwartness more than about height 1.5cm in root media and cultivates, just culture and proliferated culture medium basal culture medium are MS, culture of rootage minimal medium is 1/2MS: agar 5.0～5.5g/L, sucrose 30g/L, pH value 5.6, intensity of illumination is 15001x, light application time is 14h/d, cultivation temperature is controlled at (25 ± 1) ℃, relative air humidity is 40%～70%, test-tube seedling transplanting is chosen robust growth and is had the test-tube plantlet of more than 3 1cm left and right Xin Gen to carry out hardening, under natural daylight, tame hardening 1 week, then open bottle cap, spray clear water every day 4～5 times, after 3 days, take out seedling, clean the residual medium being attached on root, transplant in matrix, wherein said grafting matrix is humus soil mixing vermiculite, and mixed proportion is 2: 1, puts into little shed, regularly water spray, imposed one time wash fertilizer, and increases gradually illumination every 1 week, treat that nutrition pot seedling has 4～5 young leaves, when plant height 5cm is above, move into land for growing field crops cuttage,
(2) cuttage is chosen and is processed: test-tube plantlet is intercepted as upper and lower two parts, guarantee that lower partial-length is at 2-3 centimetre, upper part interval 3-4 centimetre is cut out to V-type wound with scalpel, in variable concentrations hormone, soak after 20 seconds, upper part entirety is put into square position, bury with mixed-matrix, only expose top bud, water permeable; Wherein said hormone is: heteroauxin, ABT-1 root-inducing powder, ABT-6 root-inducing powder; Described test-tube plantlet is the GiselaNo.5 of root induction phase or the group training seedling of No. 6; In described variable concentrations hormone, soak and refer to: concentration is 0.4,0.5,0.6mg/L heteroauxin, ABT-1 root-inducing powder, ABT-6 root-inducing powder;
(3) cuttage root induction: in annual spring, Qiu Liangji, cuttage on perlite mixed-matrix; Mixed-matrix is wherein perlite, vermiculite, turfy soil, river sand 2: 2: 1 by weight: 1 ratio is mixed;
(4) management after cuttage: guarantee that temperature of shed is at 26-28 ℃, humidity is at 50-60%, spray every other day carbendazim 1-2 time, after cuttage 5-7 days, spray 1-5 time time insecticide, every minor tick 2-3 days, whether observation in 10-14 days takes root, and executes a urea the last week of emerging to impel the healthy and strong growth of seedling; After 14 days, the new root of cutting begins to take shape, and after 30 days, seedling forms, and now carries out hardening, transplanting; Wherein said carbendazim refers to the aqueous solution of 1250-1670 mg/kg; Time insecticide refers to the aqueous solution of 1250-1670 mg/kg.
Embodiment 2
(1) test-tube plantlet is cultivated: first culture is chosen robust growth spring, without the tender young sprout of annual children of damage by disease and insect, with running water flow wash 60min, be transferred on superclean bench, with 75% alcohol to young sprout stem with bud sterilization 45s, then in 0.1% mercuric chloride, soak 11min, aseptic water washing 6 times, stem apex part is peeled off to the size to 1mm, remainder is cut into the stem section of 1cm left and right, be inoculated in respectively on inducing culture, under illumination condition, cultivate, inducing culture is MS+6-BA3.0mg/L+IAA0.5mg/L+ sucrose 30g/L+ agar 10g/L, pH value is 6.5, condition of culture is: intensity of illumination 3000LX, light application time 18h, 26 ℃ of temperature, incubation time 17d, cultivate through induction, can induce axillary bud sprouting, propagation is cultivated: after just for explant seedling differentiation, forward on proliferated culture medium, culture of rootage proceeds to the tender seedling of stalwartness more than about height 1.5cm in root media and cultivates, just culture and proliferated culture medium basal culture medium are MS, culture of rootage minimal medium is 1/2MS, agar 5.5g/L, sucrose 30g/L, pH value 8, intensity of illumination is 2000lx, light application time is 14h/d, cultivation temperature is controlled at (25 ± 1) ℃, relative air humidity is 40%～70%, test-tube seedling transplanting is chosen robust growth and is had the test-tube plantlet of more than 3 1cm left and right Xin Gen to carry out hardening, under natural daylight, tame hardening 1 week, then open bottle cap, spray clear water every day 4～5 times, after 3 days, take out seedling, clean the residual medium being attached on root, transplant in matrix, wherein said grafting matrix is humus soil mixing vermiculite, and mixed proportion is 2: 1, puts into little shed, regularly water spray, imposed one time wash fertilizer, and increases gradually illumination every 1 week, treat that nutrition pot seedling has 4～5 young leaves, when plant height 5cm is above, move into land for growing field crops cuttage,
(2) cuttage is chosen and is processed: test-tube plantlet is intercepted as upper and lower two parts, guarantee that lower partial-length is at 2-3 centimetre, upper part interval 3-4 centimetre is cut out to V-type wound with scalpel, in variable concentrations hormone, soak after 20 seconds, upper part entirety is put into square position, bury with mixed-matrix, only expose top bud, water permeable; Wherein said hormone is: heteroauxin, ABT-1 root-inducing powder, ABT-6 root-inducing powder; Described test-tube plantlet is the GiselaNo.5 of root induction phase or the group training seedling of No. 6; In described variable concentrations hormone, soak and refer to: concentration is 0.4,0.5,0.6mg/L heteroauxin, ABT-1 root-inducing powder, ABT-6 root-inducing powder;
(3) cuttage root induction: in annual spring, Qiu Liangji, cuttage on perlite mixed-matrix; Mixed-matrix is wherein perlite, vermiculite, turfy soil, river sand 2: 2: 1 by weight: 1 ratio is mixed;
(4) management after cuttage: guarantee that temperature of shed is at 26-28 ℃, humidity is at 50-60%, spray every other day carbendazim 1-2 time, after cuttage 5-7 days, spray 1-5 time time insecticide, every minor tick 2-3 days, whether observation in 10-14 days takes root, and executes a urea the last week of emerging to impel the healthy and strong growth of seedling; After 14 days, the new root of cutting begins to take shape, and after 30 days, seedling forms, and now carries out hardening, transplanting; Wherein said carbendazim refers to the aqueous solution of 1250-1670 mg/kg; Time insecticide refers to the aqueous solution of 1250-1670 mg/kg.
Claims (1)
1. a method for plum test-tube plantlet cuttage and quick-propagation, is characterized in that being undertaken by following step:
(1) test-tube plantlet is cultivated: first culture is chosen robust growth spring, without the tender young sprout of annual children of damage by disease and insect, with running water flow wash 30～60min, be transferred on superclean bench, with 75% alcohol to young sprout stem with bud sterilization 30～45s, then in 0.1% mercuric chloride, soak 5～11min, aseptic water washing 4～6 times, stem apex part is peeled off to the size to 0.5～1mm, remainder is cut into the stem section of 1cm left and right, be inoculated in respectively on inducing culture, under illumination condition, cultivate, inducing culture is MS+6-BA3.0mg/L+IAA0.5mg/L+ sucrose 30g/L+ agar 10g/L, pH value is 6.5, condition of culture is: intensity of illumination 3000LX, light application time 18h, 26 ℃ of temperature, incubation time 17d, cultivate through induction, can induce axillary bud sprouting, propagation is cultivated: after just for explant seedling differentiation, forward on proliferated culture medium, culture of rootage proceeds to the tender seedling of stalwartness more than about height 1.5cm in root media and cultivates, just culture and proliferated culture medium basal culture medium are MS, culture of rootage minimal medium is 1/2MS, agar 5.0～5.5g/L, sucrose 30g/L, pH value 5.6～.8, intensity of illumination is 1500～2000lx, light application time is 14h/d, cultivation temperature is controlled at (25 ± 1) ℃, relative air humidity is 40%～70%, test-tube seedling transplanting is chosen robust growth and is had the test-tube plantlet of more than 3 1cm left and right Xin Gen to carry out hardening, under natural daylight, tame hardening 1 week, then open bottle cap, spray clear water every day 4～5 times, after 3 days, take out seedling, clean the residual medium being attached on root, transplant in matrix, wherein said grafting matrix is humus soil mixing vermiculite, and mixed proportion is 2: 1, puts into little shed, regularly water spray, imposed one time wash fertilizer, and increases gradually illumination every 1 week, treat that nutrition pot seedling has 4～5 young leaves, when plant height 5cm is above, move into land for growing field crops cuttage,
(2) cuttage is chosen and is processed: test-tube plantlet is intercepted as upper and lower two parts, guarantee that lower partial-length is at 2-3 centimetre, upper part interval 3-4 centimetre is cut out to V-type wound with scalpel, in variable concentrations hormone, soak after 20 seconds, upper part entirety is put into square position, bury with mixed-matrix, only expose top bud, water permeable; Wherein said hormone is: heteroauxin, ABT-1 root-inducing powder, ABT-6 root-inducing powder; Described test-tube plantlet is the GiselaNo.5 of root induction phase or the group training seedling of No. 6; In described variable concentrations hormone, soak and refer to: concentration is 0.4,0.5,0.6mg/L heteroauxin, ABT-1 root-inducing powder, ABT-6 root-inducing powder;
(3) cuttage root induction: in annual spring, Qiu Liangji, cuttage on perlite mixed-matrix; Mixed-matrix is wherein perlite, vermiculite, turfy soil, river sand 2: 2: 1 by weight: 1 ratio is mixed;
(4) management after cuttage: guarantee that temperature of shed is at 26-28 ℃, humidity is at 50-60%, spray every other day carbendazim 1-2 time, after cuttage 5-7 days, spray 1-5 time time insecticide, every minor tick 2-3 days, whether observation in 10-14 days takes root, and executes a urea the last week of emerging to impel the healthy and strong growth of seedling; After 14 days, the new root of cutting begins to take shape, and after 30 days, seedling forms, and now carries out hardening, transplanting; Wherein said carbendazim refers to the aqueous solution of 1250-1670 mg/kg; Time insecticide refers to the aqueous solution of 1250-1670 mg/kg.
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Cited By (5)
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| CN106069326A (en) * | 2016-06-22 | 2016-11-09 | 山东省潍坊市农业科学院 | A kind of difficulty is taken root cherry rootstock Coulter asexual reproduction method |
| CN106069670A (en) * | 2016-06-22 | 2016-11-09 | 山东省潍坊市农业科学院 | Drupe fruit tree and the tender slightly cottage method of stock |
| CN106069047A (en) * | 2016-06-22 | 2016-11-09 | 山东省潍坊市农业科学院 | A kind of difficulty is taken root fruit tree asexual reproduction method |
| CN110192484A (en) * | 2018-02-27 | 2019-09-03 | 重庆市永川区花生岭李子种植专业合作社 | A kind of implantation methods of plum |
| CN110463456A (en) * | 2019-09-09 | 2019-11-19 | 新疆农业大学 | The method of wild Europe Lee's overhead layering breeding |
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| CN103416308A (en) * | 2013-08-08 | 2013-12-04 | 巴中市光雾山植物研究所 | Tissue culture rapid propagation method for wild sweet cherry trees |
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| CN102138486A (en) * | 2011-04-01 | 2011-08-03 | 天津樱桃谷农业科技发展有限公司 | Method for carrying out rapid cuttage propagation on plantlets of prunus pseudocerasus |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069326A (en) * | 2016-06-22 | 2016-11-09 | 山东省潍坊市农业科学院 | A kind of difficulty is taken root cherry rootstock Coulter asexual reproduction method |
| CN106069670A (en) * | 2016-06-22 | 2016-11-09 | 山东省潍坊市农业科学院 | Drupe fruit tree and the tender slightly cottage method of stock |
| CN106069047A (en) * | 2016-06-22 | 2016-11-09 | 山东省潍坊市农业科学院 | A kind of difficulty is taken root fruit tree asexual reproduction method |
| CN110192484A (en) * | 2018-02-27 | 2019-09-03 | 重庆市永川区花生岭李子种植专业合作社 | A kind of implantation methods of plum |
| CN110463456A (en) * | 2019-09-09 | 2019-11-19 | 新疆农业大学 | The method of wild Europe Lee's overhead layering breeding |
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