CN110463456A - The method of wild Europe Lee's overhead layering breeding - Google Patents
The method of wild Europe Lee's overhead layering breeding Download PDFInfo
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- CN110463456A CN110463456A CN201910846506.7A CN201910846506A CN110463456A CN 110463456 A CN110463456 A CN 110463456A CN 201910846506 A CN201910846506 A CN 201910846506A CN 110463456 A CN110463456 A CN 110463456A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/20—Layering
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Environmental Sciences (AREA)
- Botany (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Forests & Forestry (AREA)
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- Chemical & Material Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Wood Science & Technology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to the present invention relates to the methods of biological tissue's culture breeding, more particularly to the method for wild European Lee's overhead layering breeding, branch including first step selection no disease and pests harm, robust growth, rugosity is between 1cm~2cm, first girdling processing is carried out with grafting knife, girdling height distance press strip bifurcated mouth 15cm, girdling width are 2cm；Second step dips growth regulator with writing brush and smears girdling mouth, and affiliated growth regulator processing is 400mg/L IBA；With transparent plastic film package mixed-matrix to being wrapped up at girdling after third step 10min, wrapping is 8cm × 10cm × 5cm cylindrical body, and soil humidity is pinched agglomerating with hand, and the i.e. scattered of pine is advisable, the mixed-matrix is turf+perlite+river sand, mass ratio 1: 1: 1；4th step wraps up the time as 90d, and the present invention provides a kind of methods of wild European Lee's overhead layering breeding, and incubation rooting rate is high, provides a kind of new child care measure and Technical regulations for cultivation of tree seedlings for the protection and breeding of wild Europe Lee.
Description
Technical field
The present invention relates to the methods of biological tissue's culture breeding, and in particular to the side of wild Europe Lee's overhead layering breeding
Method.
Background technique
Xinjiang of China Altai Mountain is one of cosmopolitian plant centre of origin, has wild plant type abundant, in the area
The Wild Fruit Germplasm of large area is distributed in domain, and many types are the origin kind to cultivate apple trees.These resources have the mankind
There is highly important utility value, carry out Fruit Tree Resources research, inquiring into its origin and Evolution Paths, exploitation fruit tree new product has
Significance.
It applies within 1980 and beautiful hands over me to hold in the palm wild European Lee of discovery in the natural plants in sea in Yili of Xinjiang Xinyuan County for the first time
(Prunus domestica L.).For the presence of wild European Lee of confirmation, from morphological feature, cell chromosome and in distributed area
It is interior and nearby whether there is or not blackthorn and mirabalan etc. to be studied, it as a result proves: in the mountain wild fruits woods of Ili Prefecture day really
Wild European Lee is distributed in fact, and Yili of Xinjiang day mountain wild fruits woods is the only area of origin of European Lee.
Wild Europe Lee is shrub or dungarunga, sets high 0.8~2.8m, tree crown globular model, trunk darkviolet or Dark grey
It is glossy.Perennial branch mulberry or brown, it is stingless.Spray yellow green, hairless, no rib.Blade oval, apex is blunt nosed or has
Anxious point, base portion wedge shape, long 4~7cm, wide 3~5cm.Vein is mostly 4~5 pairs, arc-shaped to bend towards top.Leaf edge sawtooth, round blunt.
Blade face green or bottle green, hairless, blade back celadon, by pubescence.Petiole grows 0.8~1cm.Spend 2, corolla diameter 1.2~
1.5cm, grey color, 5 valves, petal are round.Calyx yellow green, calyx tube funnel-form, sepal are hairless with calyx tube.Bennet length 1.2~
1.5cm, it is hairless.Fruit oval, a small number of ellipses, mean fruit weight 4.4g, fruit indulge diameter 2.27cm, transverse diameter 1.87cm, fruit
Tip circle is convex.Suture is shallow, and two sides are symmetrical or relatively symmetrical.Pericarp background color is yellowish green, comprehensively dark violet red, have pewter fruit powder, nothing
Spot；Peel thickness is medium, and toughness is strong, and splitting is medium.Pulp yellow green, meat is soft, and juice is few, taste concentrated acid, micro-puckery, nothing
Fragrance；Soluble solid 17.7%, pH value 3, no-dehiscent fruit.Half freestone, core ellipse, core average dry weight 0.4g indulge diameter
1.6cm, transverse diameter 1.0cm, calibrate 0.6~0.7cm, and core face is coarse.Kernel has strong almond flavor.
Investigation up to now, wild Europe Lee be only distributed in my Marseille Tuo Hai, A Le of the friendship of Xinjiang, China Xinyuan County and
In the Yi Lige generation gap of its several parallel gully and Gongliu County, 1200~1300 meters of height above sea level.Arborous layer is 2~4 meters high, crown canopy
Canopy density about 0.3；Shrub has multiflora rose, sea-buckthorn, caragana, barberry, rabbit item, raspberry, hops；Herbaceous layer has reed, sweet
Grass etc..Wild Europe Lee is distributed across in natural plants, and property likes warm and moist, only preferable in warm and humid Schattenseite or waterside ability
Existence, resistance is weak, and distributed area is unstable, distribution area also very little.
The utilization of wild Europe Lee: Li Ziyuan total acid content suggests that grade scale is thought: mirabalan, wild European Lee's total acid
Content > 2.0, percentage 6.03, rank: high.Wild Europe Li Yinqi fruit is too sour, and locality is referred to as smoked plum, with Xin Jiangnan
The small smoked plum of ellipse of portion's cultivation is same, most suitable processing prune and Lee's sauce, also pharmaceutically acceptable, but European Lee's mesh of wild distribution
It is preceding not yet to develop and use.
Since reproductive capacity is lower in its natural state, distribution is seldom and environment changes and artificial destruction by wild European Lee,
Its quantity is sharply reduced, and has trend in imminent danger.Distribution has become mining site, road, Peasants Joy, pasture etc. around ground.Simultaneously because by
To the influence of the reasons such as livestock, pest and disease damage, tree vigo(u)r is compared with weak, result quantities are few.There is root turion seedling point on each distribution ground at age strain surrounding
Cloth, but the root turion seedling quantity that can grow into age is seldom, and root turion seedling is gnawed seriously by livestock.Part Wild Europe Lee's distributed point has disappeared
It dies, or only remaining several plants of withered stakes.Wild Europe Lee's seed natural propagation ability is poor, this will result directly in its diversity and is destroyed
And resource is in imminent danger.It for the current status of the resource, needs to carry out the protection and breeding to its resource from many aspects, to guarantee
The species will not become extinct, while create conditions for the development of all kinds of researchs of the species.Currently, specifically being protected for wild European Lee
It protects countermeasure and method for culturing seedlings is less, do not there is child care measure effective in detail and Technical regulations for cultivation of tree seedlings.
Summary of the invention
In view of the above problems, that the object of the present invention is to provide a kind of death rates is low, inductivity is high, callus status is good
The method of wild Europe Lee's overhead layering breeding.
The purpose of the present invention is realized by the following technical solution:
The method of wild Europe Lee's leaf tissue culture, comprising the following steps:
The first step selects the branch of no disease and pests harm, robust growth, and rugosity first carries out ring with grafting knife between 1cm~2cm
Processing, girdling height distance press strip bifurcated mouth 15cm are cut, girdling width is 2cm；
Second step dips growth regulator with writing brush and smears girdling mouth, and affiliated growth regulator processing is 400mg/
L IBA (indolebutyric acid)；
After third step 10min with transparent plastic film package mixed-matrix to being wrapped up at girdling, wrap up for 8cm ×
The cylindrical body of 10cm × 5cm, soil humidity pinched with hand it is agglomerating, pine i.e. dissipate be advisable, the mixed-matrix be turf+perlite+
River sand, mass ratio 1: 1: 1；
4th step wraps up the time as 90d.
The utility model has the advantages that
The present invention provides a kind of methods of wild European Lee's overhead layering breeding, and incubation rooting rate is high, is wild
The protection and breeding of European Lee provides a kind of new child care measure and Technical regulations for cultivation of tree seedlings.
Detailed description of the invention
The influence of Fig. 1 difference growth regulator type and concentration to overhead layering rooting rate；
Influence of Fig. 2 difference growth regulator type to overhead layering root system；
Influence of Fig. 3 different substrates to overhead layering；
Influence of Fig. 4 difference lapping to overhead layering.
Specific embodiment
The following provides a specific embodiment of the present invention, for making further details of explanation to summary of the invention.Not because
This limits the scope of the patents of the invention, all to utilize equivalent structure or equivalent process made by description of the invention and accompanying drawing content
Transformation, is applied directly or indirectly in other relevant technical fields, and is included within the scope of the present invention.
One, materials and methods
1, experimental field environment
It tests and is carried out in by the end of June, 2018 in, be experimental field located at the Xinjiang Xinyuan County township A Lemale and improve the Hetaoyuan of field three groups.
Height above sea level is 1400m or so, and soil category black loam, average temperature of the whole year is 9.60 DEG C, annual precipitation 530ml or so.
2, the screening of growth regulator type and concentration
Respectively select methyl α-naphthyl acetate (NAA) (100mg/L, 400mg/L), indolebutyric acid (IBA) (100mg/L, 400mg/L),
3 kinds of growth regulators of strength root-inducing powder (ABT root-inducing powder) (400mg/L, 800mg/L) and clear water control group (CK) are at totally 7
10 press strips are made in reason, every processing.In the branch of experimental field interior random selection no disease and pests harm, robust growth, rugosity is in 1cm~2cm
Between, girdling processing, girdling height distance press strip bifurcated mouth 15cm or so first are carried out with grafting knife, girdling width is 2cm left
It is right.Clean forming layer must be scraped when girdling but does not damage xylem [123].Then growth regulator is dipped to girdling mouth with writing brush
Smeared, after 10min with transparent plastic film package mixed-matrix to being wrapped up at girdling, wrap up for 8cm × 10cm ×
The cylindrical body of 5cm or so, soil humidity pinched with hand it is agglomerating, pine i.e. dissipate be advisable.Rooting rate, root long, root thickness and life are counted after 90d
Radical.
3, the screening of matrix
Vermiculite, turf, perlite and mixed-matrix are selected altogether, and mixed-matrix is turf, perlite, river sand, and mass ratio is
=1: 1: Isosorbide-5-Nitrae kind matrix is tested.Branch is carried out with grafting knife uniformly to smear 800mg/L with writing brush after girdling processing
ABT root-inducing powder wraps up different substrates using transparent plastic film after 10min respectively and is wrapped up.10 press strips are made in every processing.
Rooting rate, root long, root thickness and number of taking root are counted after 90d.
4, the screening of lapping
800mg/L ABT root-inducing powder is uniformly smeared with writing brush after carrying out girdling processing to branch with grafting knife, is used after 10min
Different lappings wrap up mixed-matrix respectively and are wrapped up.Made altogether using 3 kinds of transparent plastic film, non-woven fabrics and black film materials
For overhead layering lapping, every kind of material makees 10 press strips.Rooting rate, root long, root thickness and number of taking root are counted after 90d.
Two, test result
1, the influence of different growth regulator types and concentration to overhead layering
As shown in Figure 1, the overhead layering of variety classes growth regulator processing can take root, and callus rate is higher.Its
In 400 mg/L NAA, 400mg/L IBA and 800mg/L ABT root-inducing powder processing press strip rooting rate be 20.00%.
400mg/L IBA, 400mg/L ABT and the press strip callus rate of CK processing are up to 100.00%, but the press strip rooting rate of CK processing is
0, illustrate that different types of growth regulator can promote wild European Lee's overhead layering to take root, but rooting rate is not high.
As shown in Figure 2, different growth regulator types and the press strip root growth of concentration processing differ greatly, wherein IBA
The press strip root long of processing is 38.74mm, is secondly press strip root system more a length of 15.41mm, the NAA processing of ABT root-inducing powder processing
It is only 4.27mm that root system is most short；It is little in root thickness and influence of the 3 kinds of growth regulators of quantitative aspects to press strip of taking root, wherein IBA
The press strip root system of processing is most slightly 2.13mm, and quantity of taking root is 2, and the press strip root system rugosity of NAA processing is most carefully 1.06mm, only
There is 1 root system.IBA, which is more advantageous to, in 3 kinds of growth regulators promotes overhead layering root growth, but root system entirety slow growth,
It is ineffective.
2, influence of the different substrates to overhead layering
From the figure 3, it may be seen that press strip callus rate is up to 100.00% when being used as package matrix using turf and perlite, use
Callus rate is 80.00% when vermiculite is as package matrix, and the press strip callus rate of the death rate 20.00%, mixed-matrix is
60.00%.In 4 kinds of package matrix, the press strip rooting rate with turf, vermiculite and perlite package is 0, only mixed base
The press strip of matter package can take root, but rooting rate is only 20.00%.
3, influence of the different lappings to overhead layering
As shown in Figure 4, non-woven fabrics and black film is used to make the press strip death rate of lapping as 100.00%, rooting rate 0,
The press strip of only plastic film package can take root, and rooting rate 10.00%, other than sporadic deaths, remaining press strip all may be used
To form callus.It finds during the test, when making lapping using black film and non-woven fabrics, due to summer high temperature, and 2
Person is black material, and heat absorptivity is stronger, and overhead layering substrate temperature is unfavorable for root growth up to 32 DEG C or more, in addition, by
Stronger in non-woven fabrics gas permeability, dehydration comparatively fast leads to the dry water shortage of matrix, is unfavorable for taking root.
Three, it analyzes
1, influence of the different press strip periods to overhead layering
The press strip period of different tree species is different, and survival rate can be improved in suitable press strip period, at this time ambient temperature and humidity, branch
Nutrient, endogenous growth adjust agent content etc. be all it is best, be conducive to take root.The study found that press strip time and root growth shape
Condition is related, it is necessary to according to the different tree species suitable control press strip time.The discovery press strip time is to wild European Lee fruit during test
Tree overhead layering is affected, for example in mid-July, 2017, press strip was not after the overhead layering preliminary experiment 90d experimental field done
It takes root, part press strip is dead, and remaining press strip only forms callus, and the overhead layering carried out in late June, 2018 is tested
Press strip can take root after 90d.Guess may be due to the preliminary experiment press strip time too late, inside press strip contained by growth regulator it is dense
Spend lower, and to mid-October after 90d, temperature is lower, is unfavorable for root system and is formed.
2, different carve hurts influence of the mode to overhead layering
In test other than being screened to growth regulator, matrix, lapping, also to overhead layering using band girdle,
Ring carve 2 kinds quarter the mode of hurting compare.The result shows that ring, which is carved, can form callus with press strip after band girdle, but ring is carved
Press strip only forms callus afterwards, and callus is filled with quarter traumatic part position and press strip forming layer is caused to heal, and can not form root system,
And the press strip after band girdle not only forms callus, finally yet forms root system.In addition, not only ring quarter is easy to cause callus group
Accumulation forming layer healing is knitted, band girdle width is too short to be also easy so that callus fills up wound, therefore in next research
Plan can focus on test on band girdle width and the screening of press strip time.
Four, conclusion
Wild Europe Lee's overhead layering preferably growth regulator processing is taken root to be smeared using 400mg/L IBA
Rate is higher, and root growth is preferable；Preferably package matrix is turf+perlite+river sand, the mixed-matrix of mass ratio 1: 1: 1；
Relative to non-woven fabrics and black film, transparent plastic film is more suitable for the lapping of wild European Lee's overhead layering.
Claims (1)
1. the method for wild Europe Lee's overhead layering breeding, comprising the following steps:
The first step selects the branch of no disease and pests harm, robust growth, and rugosity is first carried out at girdling with grafting knife between 1cm~2cm
Reason, girdling height distance press strip bifurcated mouth 15cm, girdling width are 2cm；
Second step dips growth regulator with writing brush and smears girdling mouth, and affiliated growth regulator processing is 400mg/L
IBA (indolebutyric acid)；
After third step 10min with transparent plastic film package mixed-matrix to being wrapped up at girdling, wrap up for 8cm × 10cm ×
The cylindrical body of 5cm, soil humidity pinched with hand it is agglomerating, pine i.e. dissipate be advisable, the mixed-matrix be turf+perlite+river sand, matter
Amount is than being 1: 1: 1；
4th step wraps up the time as 90d.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114617063A (en) * | 2022-02-23 | 2022-06-14 | 中国林业科学研究院热带林业研究所 | Method for obtaining aquilaria sinensis new green machilus clone strain |
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| CN114617063A (en) * | 2022-02-23 | 2022-06-14 | 中国林业科学研究院热带林业研究所 | Method for obtaining aquilaria sinensis new green machilus clone strain |
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Application publication date: 20191119 |