CN105230433A - Cutting propagation method for American red maples - Google Patents
Cutting propagation method for American red maples Download PDFInfo
- Publication number
- CN105230433A CN105230433A CN201510778110.5A CN201510778110A CN105230433A CN 105230433 A CN105230433 A CN 105230433A CN 201510778110 A CN201510778110 A CN 201510778110A CN 105230433 A CN105230433 A CN 105230433A
- Authority
- CN
- China
- Prior art keywords
- cuttage
- cuttings
- cutting
- root
- american red
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a cutting propagation method for American red maples. The method comprises the steps that 1, 7-10 days before cutting is performed, deep plowing is performed on matrixes in a cutting pool to shovel sundries and newly grown weeds in the cutting pool, the cutting pool is exposed to the sun for 1-2 days, and then the cutting matrixes are sterilized and disinfected; 2, branches of one-year seedlings or two-year seedlings are selected, materials are drawn from the upper portions, the middle portions and the lower portions of the seedlings to obtain cutting slips, upper notches of the cutting slips are sealed with wax or smeared with vaseline, and lower notches of the cutting slips are soaked with ABT for 30 min; 3, the treated cutting slips are cut into the cutting pool, callus tissue is grown 7-12 days after cutting is performed, adventitious roots are generated one week after the callus tissue is grown, and then the cutting slips are in a rooting period and a massive rooting period. According to the cutting propagation method for the American red maples, the lower portion branches of one-year stock plants serve as the cutting slips, are soaked in 500 ppm of an ABT solution for half an hour and then are cut in the matrixes, the rooting rate is the highest and reach 83.33 percent, the rooting number is 13.10, and the root length is 6.88 cm.
Description
Technical field
The present invention relates to American red-maple raising technology field, be specifically related to the cuttage breeding method using a kind of American red-maple.
Background technology
American red-maple (Acerrubrum), has another name called red maple, Aceraceae Acer, and autumn, look gorgeous was as flower, was one of most popular green tree species of the U.S., and at present, market is also very powerful and exceedingly arrogant at home.Its resistance, wide accommodation, landscape effect is good, and American red-maple, from the young leaves in spring and flowers and fruits to the gorgeous leaf look in autumn, all has ornamental value.For keeping the merit of American red-maple, the vegetative manner such as cuttage, tissue cultures can only be taked to breed.
Cuttage is undertaken planting breeding by the branch of trees or a part for stem, but the material of cuttage is not limited to the limb of trees, other parts are root, leaf, bud such as, and herbal tender stem, young shoot, subterranean stem etc., all can be formed completely independently individual, and often the cuttage of these different materials is called stem cutting, stem is inserted, root is inserted, leaf is inserted and bud cutting.The environment such as the rooting process of cuttings and the temperature of surrounding, humidity, illumination, air and matrix have close contacting, and the external factor affecting cutting survival comprises:
(1) slotting machine matrix is the place of cuttage rooting, needs sufficient water and air.Surviving of cuttings needs enough moisture, but water is many, and intramatrical air will reduce.In order to solve this contradiction, slotting machine has the structure of oneself uniqueness.First should to be selected in physical features higher for slotting machine, and well-drained place, the surrounding of bed is with brick.Bottom leaves many places osculum, is beneficial to unnecessary moisture and freely discharges.
(2) moisture moisture is the key factor of cuttage rooting, will provide sufficient moisture to cuttings, and is made to take root reached desirable level by the adjustment that is used for of water.
(3) temperature affects the physiological activity such as cell division and breathing of cuttings, thus affects the regeneration event of cuttings.Favors low temperature, in the formation of callus, roots with 20 ~ 30 DEG C as optimum.Heat the speed that also can promote to survive in seedbed.
(4) energy of light only photosynthesis of plant, influence of light photosynthesis and affect formation and the growth of adventive root.
(5) oxygen in air slotting machine and cuttage rooting relation are very close, and when oxygen concentration is moderate, rooting efficiency is best, takes root and weaken or stop to take root when oxygen concentration is too low.Good air permeability should be selected, the cutting medium that drainage is good in cuttage.
(6) plant that hormone is different is different with reaction to the requirement of growth hormone, the object that therefore will reach hestening rooting will use suitable concentration and dosage, otherwise can not expected effect be there is, excessive concentration can produce the effect suppressing to take root, so the auxin concentration be applicable to, be that cuttage is successfully crucial.Plant hormone plays vital effect in the formation of adventive root, and the rooting percent, transplanting survival rate, height of seedling, leading thread etc. crossed through HORMONE TREATMENT have and generally improve.Conventional somatotropin has 2,4-D, methyl α-naphthyl acetate, heteroauxin, indolebutyric acid, root-inducing powder etc., and herbaceous plant uses low concentration, the plant that general lignification and being difficult to is taken root, and applicable concentration wants high.
The rootability of cuttings not only has very big-difference in the section of plant belongs to, and also shows difference between kind.The kind of the rootability of cuttage and cuttings itself, upgrowth situation etc. have close relationship, and these factors can affect the survival rate that can cuttage reach ideal.The internal factor affecting cuttage comprises:
(1) plant genotype plant is because genotypic difference, and in growth and development process, have respective physiology individual character, the plant be embodied in more easily takes root, as willow, willow, what have is slightly difficult, and taking root of having has larger difficulty, even do not take root, as the certain plants of walnut section.
(2) there is two layers of meaning at the cuttings age at cuttings age: one is the elite stand age of taking cutting, and one is age of fringe bar itself.The general elite stand age is less, takes root easier.Branch raw then on elite stand is because Endogenous Hormone Contents in Vitro is high, cell division activity is easily taken root by force and comparatively.And with advancing age, the metabolic capability of plant weakens, the material of rooting of cuttings is suppressed to increase.
(3) quality of cuttings quality branch development directly has influence on the number of nutriment in branch, this takes root for surviving have a great impact for cuttings, fringe bar Middle nutrition abundance is the basis of neologism nutriment synthesis after cuttage, growth is enriched, nutritious cuttings is easily taken root, too young tender shoot tissue is not well established, after cuttage, cutting nutrition is few, and resistance is poor, easily rotten death.
(4) adopt the branch that fringe position is taked at the different parts of elite stand, performance of taking root is discrepant.This part of below general ground floor major branch base portion to trunk and collar portion, belongs to maximum by the age, but the branch that it is sprouted is the juvenile phase.And the peripheral branch of tree crown, though the age of tree is little, be in the stage of ripeness.Branch development on general species main shaft is best, and meristematic capacity is strong, is better than side shoot with this, is especially better than repeatedly the side shoot of branch as cuttings.The sprout tillers bar grown from collar position is as cuttings, more favourable to taking root.
(5) length of the size cuttings of cuttings and the rooting rate of thickness to plant have a certain impact, and general cuttings is larger, and inner organic nutrient substance is more, and cuttage is then more easily taken root, but exceed certain rugosity and can weaken by rooting rate on the contrary.The rootability of cuttings increases with the increase of length, but exceed certain length, and rooting rate declines on the contrary.
(6) the leaf area leaf of cuttings is to provide to take root and promotes the source of material, if extractd by whole leaves during cuttage, the survival rate of fringe bar obviously declines.Generally speaking leaf carries out photosynthesis promotion plant growth and takes root, but for reducing rising dehydration, should remove side blade, retains growing way good side blade and carries out photosynthesis.
American red-maple belongs to cortex and to take root seeds, though conventional method can be taken root, but survival rate is extremely low, so need to adopt different exogenous hormones and suitable concentration to take root to it to carry out facilitation, improves the cuttage survival rate of American red-maple.Suitable selection position and the selection at elite stand age, must impel the rooting rate of American red-maple cuttage to improve.If the method for American red-maple Technique on Cuttage Propagation is proper, extensive, high-effective root-growing can be reached.
Summary of the invention
Goal of the invention: for the deficiencies in the prior art, the object of this invention is to provide a kind of cuttage breeding method of American red-maple, with the hard branch of American red-maple for cuttage material, improves rooting rate.
Technical scheme: for achieving the above object, the technical solution used in the present invention is as follows:
A cuttage breeding method for American red-maple, comprises the steps:
1) matrix in cuttage pond was ploughed deeply in before cuttage 7 ~ 10 days, foreign material wherein, the weeds that newly grow are rooted out, is exposed to the sun under sunlight 1 ~ 2 day, then sterilization is carried out to cutting medium;
2) choose robust growth, grow normal, without the branch of the full one year seedling of damage by disease and insect, eye or 2 years raw seedlings; From the top of nursery stock, draw materials obtained cuttings in middle part or bottom; At cuttings sealing wax suitable for reading or smear with vaseline, lower cut ABT immersion treatment 30min;
3) cuttings handled well is inserted in cuttage pond, water once permeable after inserting, with the surface of a wound close to dropping down water for degree; Namely full light break spraying apparatus is brought into use to carry out the control of temperature and humidity after inserting; After cuttage, within 7 ~ 12 days, grow callus, and produce adventive root after thereafter week, enter the phase of taking root afterwards, take root the phase in a large number.
Step 1) in, medicament adopts the carbendazim solution of 0.5%, sprays bed surface, infiltrates the matrix in seedbed, is covered, raise film after 7 ~ 10 days, carry out cuttage with plastic film.
Step 2) in, the long 10 ~ 12cm of cuttings, stays 2 ~ 3 joints, upper cut straight snips, and apart from first bud about 1cm, lower cut is cut into inclined-plane or shape of a hoof tangent plane near under joint, and clip is level and smooth.
Step 2) in, by the classification of cuttings thickness, basically identical 30 of rugosity are bundled into a bundle, cover wet cloth, In Shade.
Step 3) in, cuttage seeding row spacing is 8 × 15cm, and 1/2 ~ 2/3 of cuttings base portion inserts slotting machine; The wooden stick that prior use is slightly coarser than cuttings punches on slotting machine, real with hand rammer after insertion, and cuttings is combined closely with seedbed.
Step 3) in, spray amount is blade face to hang with all the time sparkling and crystal-clear water droplet for degree; Fine day notes cooling noon, meets high temperature and strengthens injection flow rate, and shade to cuttage seedbed with sunshade net, at overcast and rainy minimizing injection flow rate or water spray number of times.
The cuttage breeding method of described American red-maple, with annual maternal plant bottom branch for cuttings, in the ABT solution of 500ppm, soak 30min, cuttage is taken root in matrix.
Beneficial effect: compared with prior art, the present invention has the following advantages:
1) the present invention adopts cuttage, and compared with existing epicormic branch cuttage, adopt cuttage can avoid summer high temperature high humidity, comparatively epicormic branch cuttage is low for the rotting rate of cuttage; Adopt cuttage can reduce artificial high temperature work (weeding, statistics and observation), reduce the number of times of behavior adjustment management, using water wisely, reduce water spray number of times.
2) American red-maple cottage propagation solves the shortcoming that tree seedling variation is large and the cycle is long, maintains the merit of American red-maple female parent, the genotype that leaf look gorgeous, the a large amount of Fast-propagation of energy, seedling raise period is short, simple to operate, and easy large-scale promotion implements and propagating materials is sufficient.
3) with annual maternal plant bottom branch for cuttings, in the ABT solution of 500ppm, soak half an hour, cuttage is in matrix, and rooting rate is high, reaches 83.33%, and number of taking root is 13.10, and root length is 6.88cm.
Accompanying drawing explanation
Fig. 1 is the rooting rate comparison diagram of different seedling age cuttings;
Fig. 2 is the root length comparison diagram of different seedling age cuttings;
Fig. 3 is several comparison diagram of taking root of different seedling age cuttings;
Fig. 4 is the impact of different biopsy method position on each index of cuttage rooting.
Embodiment:
Below in conjunction with specific embodiment, the invention will be further elaborated.Embodiment is for explanation and unrestricted the present invention.Be intended to be convenient to any those of ordinary skill in this area and can understand these embodiments, do not limit the present invention in any way, suitable amendment can be made and without prejudice to essence of the present invention and depart from scope of the present invention.
Embodiment 1
Test material: test and carry out in full sunshine and spray cuttage pond, Nanjing Forestry University ginkgo garden, cutting medium is perlite and fine husky mixture (1:1), with the annual seedling of American red-maple and 2 years raw seedlings for cottage propagation material.
Slotting machine prepares: within 7 ~ 10 days, plough deeply matrix in cuttage pond before the test, foreign material wherein, the weeds that newly grow is rooted out, is exposed to the sun 1 ~ 2 day, then carries out sterilization to cutting medium under sunlight.Medicament adopts the carbendazim solution of 0.5%, sprays bed surface, infiltrates the matrix in seedbed, is covered, raise film after 7 ~ 10 days, carry out Cutting test with plastic film.
The acquisition and processing of cuttings: choose robust growth, grow normal, without the full branch of damage by disease and insect, eye.Respectively from the top of nursery stock, draw materials in middle part and bottom.Cuttings is about 10 ~ 12cm, stays 2 ~ 3 joints, upper cut straight snips, and apart from first bud about 1cm, lower cut is cut into inclined-plane or shape of a hoof tangent plane near under joint, ensures that clip is level and smooth.By the classification of cuttings thickness, basically identical 30 of rugosity are bundled into a bundle, cover wet cloth, In Shade.Then sealing wax suitable for reading or smear with vaseline, lower cut carries out the immersion treatment of different growth regulators according to the method for experimental scheme.
Cuttage and management after inserting: the cuttings grouping handled well is inserted in cuttage pond, cuttage seeding row spacing is 8 × 15cm, and 1/2 ~ 2/3 of cuttings base portion inserts slotting machine, punches in advance with the wooden stick being slightly coarser than cuttings on slotting machine, real with hand rammer after insertion, cuttings is combined closely with seedbed.Water once permeable after inserting, with the surface of a wound close to dropping down water for degree.Namely bring into use full light break spraying apparatus to carry out the control of temperature and humidity after inserting, spray amount is blade face to hang with all the time sparkling and crystal-clear water droplet for degree.Fine day notes cooling noon, and every day, 10:00 ~ 17:00, in case of high temperature strengthens injection flow rate, and shaded to cuttage seedbed with sunshade net, at overcast and rainy minimizing injection flow rate or water spray number of times, keeps certain temp. and humidity to take the circumstances into consideration to consider with blade face.
Observe and statistical method: cuttage starts the apparition of observing calli induction situation and adventive root after one week, within every 3 ~ 5 days afterwards, observe once, to wound healing, grow new root.The situation of taking root of cuttage 2 months " Invest, Then Investigate " cuttings.Testing index for treatment combination comprises: callus apparition (d), adventive root apparition (d), rooting rate (%), take root number (bar) and adventive root (cm) etc.Adopt Excel to carry out data analysis, statistical method SPSS software carries out multi-contrast, significance test and variance analysis etc.
Experimental scheme: this test adopts the hard branch of American red-maple in spring to carry out cuttage, adopt the method for 4 way crossover study designs, B (position of drawing materials), C (hormone kind), D (HORMONE TREATMENT mode) three factors adopt three level design, A (seedling age) level is because have two kinds of seedling ages, and adopt the design of two kinds of levels, the design table (schedule) of each factor of orthogonal experiment sees the following form 1.
The factor of the orthogonal experiment of table 1 American red-maple cuttage and level
Cutting test design is as shown in table 2.Each process 30 cuttings, repeat for 3 times.Adopt life in 1 year and life in 2 years two kinds of nursery stocks, the HORMONE TREATMENT that IBA, NAA, ABT tri-kinds is different, concentration is respectively 250mg/L, 500mg/L and 1000mg/L, the time of answering with three relative concentrations is the 250mg/L concentration processing time is one hour, the 500mg/L concentration processing time is half an hour, and the 1000mg/L concentration processing time is for dip in 10s soon.1 year raw seedling, 9 treatment combinations, corresponding 2 years raw seedlings, 9 treatment combinations, total 18 treatment combinations.
The Orthogonal Experiment and Design of table 2 American red-maple cuttage
| Numbering | A (seedling age) | B (position of drawing materials) | C (hormone kind) | D (HORMONE TREATMENT mode) |
| 1 | 1 (2 years raw seedlings) | 1 (top) | 1(IBA) | 1(250ppm,1h) |
| 2 | 1 | 1 | 2(NAA) | 2(500ppm,0.5h) |
| 3 | 1 | 1 | 3(ABT) | 3(1000ppm,10s) |
| 4 | 1 | 2 (middle parts) | 1 | 2 |
| 5 | 1 | 2 | 2 | 3 |
| 6 | 1 | 2 | 3 | 1 |
| 7 | 1 | 3 (bottoms) | 1 | 3 |
| 8 | 1 | 3 | 2 | 1 |
| 9 | 1 | 3 | 3 | 2 |
| 1′ | 2 (one year seedlings) | 1 | 1 | 1 |
| 2′ | 2 | 1 | 2 | 2 |
| 3′ | 2 | 1 | 3 | 3 |
| 4′ | 2 | 2 | 1 | 2 |
| 5′ | 2 | 2 | 2 | 3 |
| 6′ | 2 | 2 | 3 | 1 |
| 7′ | 2 | 3 | 1 | 3 |
| 8′ | 2 | 3 | 2 | 1 |
| 9′ | 2 | 3 | 3 | 2 |
After cuttage, within 7 ~ 12 days, grow callus, and produce adventive root after thereafter week, enter the phase of taking root afterwards, take root the phase in a large number.Period, the 9 ' treatment combination of one year seedling produces callus and adventive root is the fastest, and being about 7 days, is secondly No. 9 combinations of 2 years raw seedlings, is about 12 days.Produce callus the slowest be the cuttings on the top of taking from one year seedling, the cuttings taking from plant top is generally at eustipes part root of hair, because band terminal bud, growth hormone transports toward lower end, is gathered in eustipes part root of hair.Part cuttings incision has callus to occur, occurs the translucent callus of milky, in spot-like projections along incision.Without callus, and there is spot-like projections below the eustipes part near base portion in some cuttings incision.
As shown in Figure 1, annual and 2 years raw seedlings have larger difference in rooting rate, and compared with 2 years, the rooting rate of raw seedling was high in rooting rate for one year seedling.The cuttings taking from two kinds of seedling ages cannot directly judge in root length, and as shown in Figure 2, the root length of great majority 2 years raw seedling cuttings is greater than the root length of one year seedling cuttings to result.As shown in Figure 3, the number of taking root of one year seedling is better than the number of on average taking root of 2 years raw seedlings.In general, select the rooting rate of one year seedling higher.
The branch situation of taking root taking from plant different parts differs greatly (as shown in Figure 4), takes from the branch of plant bottom, and its rooting efficiency is obviously better than taking from the branch with top in the middle part of plant.And the branch taken from the middle part of plant carries out cuttage, its rooting efficiency is also better than the branch taking from plant top.The average rooting rate taking from plant bottom branch is 55.47%, and number of on average taking root is 10.99, and average root is long is 6.96cm.The average rooting rate taking from the branch on plant top only 13.89%, its number of taking root is 6.40, and root is long is 3.85cm.And the branch taken from the middle part of plant, its all kinds of index is also in medium level.
The cuttage rooting proterties taking from plant bottom in this test is good, is that in the winter time during dormancy, the nutrition content of storage is many because the cuttings of close base portion.During cuttage in spring, the existence of this part nutriment, makes the resistance of cuttings strengthen.Meanwhile, these nutriments also contribute to cuttings as early as possible breaking dormancy start to sprout, the bud of sprouting constantly to cuttings base portion transport growth hormone, can accelerate the cell division of cuttings otch, cicatrizes a wound, prevent from rotting, promote the formation of root.
Table 3 variety classes HORMONE TREATMENT is on the impact of cuttage rooting
Note: significant difference (P<0.05) between different letter representation process, lower same.
Different types of HORMONE TREATMENT is to the situation of taking root of cuttings, and result is as shown in table 3, and the short the most obvious hormone of root effect is ABT, its five indexs of taking root are all better, the rooting percent urging root with ABT is up to 35.79%, and its number of taking root is 8.52, and root length is 5.68cm.This is higher than the average level by IBA and NAA HORMONE TREATMENT cuttings.More then there is some deviations in hormone IBA and NAA, with the average rooting rate of IBA process cuttings higher than with NAA process, and number of taking root, root length, root system exponential sum root system efficiency index these four refer to that target value is lower than the cuttings with NAA process.
Table 4 hormon process processing mode is on the impact of cuttage rooting
Different HORMONE TREATMENT modes to be taken root number and the situation of taking root such as root length to rooting percent, on average, and result is as shown in table 4, and the hormone solution immersion half an hour of 500ppm concentration in this test, its average rooting rate reaches 34.31%, for the highest.Meanwhile, the average of all other rooting inhibitors is also better than the index of correlation under other two kinds of HORMONE TREATMENT modes.On average take root number for the highest 8.53, and average root length also be the 5.37cm of the best, and root system index is 7.74, and root system efficiency index is 3.33 best.Under 250ppm, 1h immersion treatment, five rooting inhibitors of cuttings not all dip in the rooting inhibitors of the lower cuttings of process soon higher than 1000ppm, 10s.Descend the average rooting rate of cuttings and root system efficiency index to be better than 1000ppm in 250ppm, 1h process, 10s dips in the index of correlation of process soon.
The different processing mode of table 5 is on the impact of American red-maple cuttage root-taking
| Sequence number | Rooting rate (%) | Take root and count (bar) | Root long (cm) |
| 1 | 13.33±1.04i | 3.20±0.20k | 2.60±0.10j |
| 2 | 6.67±1.23k | 4.53±0.37j | 4.90±0.10ef |
| 3 | 8.00±0.11jk | 7.24±0.56hi | 3.82±0.28hi |
| 4 | 22.58±1.00fg | 3.12±0.38k | 3.71±0.11hi |
| 5 | 15.15±1.56hi | 6.54±0.36i | 4.85±0.15efg |
| 6 | 13.33±1.17i | 4.23±0.67j | 5.10±0.20de |
| 7 | 47.50±0.80d | 8.32±0.58fg | 7.50±0.06b |
| 8 | 51.72±1.88cd | 10.21±0.79c | 7.68±0.30b |
| 9 | 57.14±3.18b | 12.11±0.69b | 8.88±0.48a |
| 1′ | 16.67±0.87i | 7.49±0.41gh | 3.52±0.78i |
| 2′ | 12.00±1.70ij | 9.32±0.48de | 3.67±0.17hi |
| 3′ | 26.67±1.14f | 6.62±0.42i | 4.59±0.21efg |
| 4′ | 24.14±0.54fg | 9.00±0.40ef | 4.20±0.13gh |
| 5′ | 20.00±2.30gh | 7.68±0.12gh | 4.33±0.27fgh |
| 6′ | 25.71±1.83f | 7.81±0.09gh | 4.78±0.28efg |
| 7′ | 40.00±2.40e | 12.21±0.29b | 5.20±0.12de |
| 8′ | 53.13±3.20bc | 10.00±0.16cd | 5.60±0.16d |
| 9′ | 83.33±3.00a | 13.10±0.70a | 6.88±1.02c |
18 different processing modes are on the impact of cuttage, and the results are shown in Table 5, what wherein rooting rate was the highest is 9 ': maternal plant is one year seedling, takes from the cuttings of plant bottom, and it is best that the solution of ABT500ppm soaks the rooting efficiency of half an hour.Next is No. 9, namely adopts 2 years raw seedlings, takes from the cuttings of plant bottom, soak the rooting efficiency of half an hour better in the ABT solution of 500ppm.9 ' and No. 9 process at rooting rate and number two level differences significantly (P<0.05) of taking root.9 ' number rooting rate is up to 83.33%, and its number of taking root also is maximum, 13.10; Next is No. 9 rooting rates is 53.14%, and number of taking root is 12.11; Be 8 ' number rooting rate be again 53.13%, number of taking root is 10.00.No. 2 process that in 18 treatment combinations, rooting rate is the poorest, rooting rate is only 6.67%.With rooting rate and take root number this two indices unlike, what root length was the longest is No. 9 combinations, trace it to its cause, No. 9 process cuttings used are the cuttings of 2 years raw seedlings, it experienced by longer development time, nutriment and various anthocyanin, the enzyme etc. of more horn of plenty are contained in cuttings, to its root growth also advantageously, therefore, when other processes consistent, the average root length of No. 9 process of 2 years raw seedlings is just drawn materials higher than same, same hormone, the average root length of 9 ' number process under same HORMONE TREATMENT mode.
Table 6 different disposal is to the range analysis of rooting percent
| Kij | A | B | C | D |
| K 1 | 235.43 | 83.33 | 164.22 | 173.90 |
| K 2 | 301.64 | 120.92 | 158.67 | 205.86 |
| K 3 | 332.83 | 214.19 | 157.32 | |
| X 1 | 26.16 | 13.89 | 27.37 | 28.98 |
| X 2 | 33.52 | 20.15 | 26.44 | 34.31 |
| X 3 | 55.47 | 35.70 | 26.22 | |
| R | 7.36 | 41.58 | 9.25 | 8.09 |
| Excellent level | A 2 | B 3 | C 3 | D 2 |
The range analysis of orthogonal experiment is in table 6, and each factor level is different, differing greatly between process.At A
2b
3c
3d
2under combination, namely choose the bottom branch of one year seedling, in the ABT solution of 500ppm, soak half an hour, the rooting rate of American red-maple cuttings is the highest, is 83.33%, and the number of on average taking root of cuttings is also the highest 13.10.The factor that R is maximum, its change level is maximum, is the principal element of influence index.In this test, the maximum factor of extreme difference is position (B) of drawing materials, next is hormone kind (C), with processing mode (D), affecting minimum is seedling age (A), so 4 factors are B>C>D>A on the primary-slave relation that cuttage survival rate also affects.
18 treatment combinations in test, with last process: A
2b
3c
3d
2for best, survival rate is to 83.33%.Through range analysis, factor level A is with A
2as well, factor level B is with B for level
3as well, factor level C is with C for level
3as well, this horizontal D of factor is with D for level
2level as well, so the result of range analysis also shows that the optimum level of 4 factors is combined as A
2b
3c
3d
2, this is consistent with the result of test.Therefore this test can think that raising American red-maple cuttage survival rate effect is it is preferred that the branch taking from 1 year raw seedling plant bottom soaks half an hour in the ABT of 500ppm.
Claims (7)
1. a cuttage breeding method for American red-maple, is characterized in that, comprises the steps:
1) matrix in cuttage pond was ploughed deeply in before cuttage 7 ~ 10 days, foreign material wherein, the weeds that newly grow are rooted out, is exposed to the sun under sunlight 1 ~ 2 day, then sterilization is carried out to cutting medium;
2) choose robust growth, grow normal, without the branch of the full one year seedling of damage by disease and insect, eye or 2 years raw seedlings; From the top of nursery stock, draw materials obtained cuttings in middle part or bottom; At cuttings sealing wax suitable for reading or smear with vaseline, lower cut ABT immersion treatment 30min;
3) cuttings handled well is inserted in cuttage pond, water once permeable after inserting, with the surface of a wound close to dropping down water for degree; Namely full light break spraying apparatus is brought into use to carry out the control of temperature and humidity after inserting; After cuttage, within 7 ~ 12 days, grow callus, and produce adventive root after thereafter week, enter the phase of taking root afterwards, take root the phase in a large number.
2. the cuttage breeding method of American red-maple according to claim 1, is characterized in that: in step 1), and medicament adopts the carbendazim solution of 0.5%, bed surface is sprayed, infiltrates the matrix in seedbed, covered with plastic film, raise film after 7 ~ 10 days, carry out cuttage.
3. the cuttage breeding method of American red-maple according to claim 1, is characterized in that: step 2) in, the long 10 ~ 12cm of cuttings, stay 2 ~ 3 joints, upper cut straight snips, apart from first bud about 1cm, lower cut is cut into inclined-plane or shape of a hoof tangent plane near under joint, and clip is level and smooth.
4. the cuttage breeding method of American red-maple according to claim 1 and 2, is characterized in that: step 2) in, by the classification of cuttings thickness, basically identical 30 of rugosity are bundled into a bundle, cover wet cloth, In Shade.
5. the cuttage breeding method of American red-maple according to claim 1, is characterized in that: in step 3), and cuttage seeding row spacing is 8 × 15cm, and 1/2 ~ 2/3 of cuttings base portion inserts slotting machine; The wooden stick that prior use is slightly coarser than cuttings punches on slotting machine, real with hand rammer after insertion, and cuttings is combined closely with seedbed.
6. the cuttage breeding method of American red-maple according to claim 1, is characterized in that: in step 3), and spray amount is blade face to hang with all the time sparkling and crystal-clear water droplet for degree; Fine day notes cooling noon, meets high temperature and strengthens injection flow rate, and shade to cuttage seedbed with sunshade net, at overcast and rainy minimizing injection flow rate or water spray number of times.
7. the cuttage breeding method of American red-maple according to claim 1, is characterized in that: with annual maternal plant bottom branch for cuttings, in the ABT solution of 500ppm, soak 30min, cuttage is taken root in matrix.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510778110.5A CN105230433A (en) | 2015-11-13 | 2015-11-13 | Cutting propagation method for American red maples |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510778110.5A CN105230433A (en) | 2015-11-13 | 2015-11-13 | Cutting propagation method for American red maples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105230433A true CN105230433A (en) | 2016-01-13 |
Family
ID=55028505
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510778110.5A Pending CN105230433A (en) | 2015-11-13 | 2015-11-13 | Cutting propagation method for American red maples |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105230433A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106416680A (en) * | 2016-09-08 | 2017-02-22 | 广西壮族自治区林业科学研究院 | Melaleuca alternifolia cutting propagation method |
| CN107047203A (en) * | 2017-05-10 | 2017-08-18 | 蚌埠王恒亮盆景艺术有限公司 | A kind of trident maple cuttage seeding cultivating method |
| CN113875409A (en) * | 2021-10-14 | 2022-01-04 | 山东农业大学 | Efficient cutting propagation method for holly |
| CN114793653A (en) * | 2022-04-19 | 2022-07-29 | 广西壮族自治区南宁良凤江国家森林公园 | Litsea cubeba hard branch cutting propagation method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1754007A1 (en) * | 1989-12-19 | 1992-08-15 | Н.Ф. Серегин | Method of fruit crop multiplication |
| CN103081683A (en) * | 2013-01-22 | 2013-05-08 | 沈阳农业大学 | Improved American red maple twig cutting propagation method |
| CN103563617A (en) * | 2013-10-12 | 2014-02-12 | 杭州市园林绿化股份有限公司 | Red maple cuttage breeding method |
| CN104322240A (en) * | 2014-09-05 | 2015-02-04 | 郎溪县凌笪乡永辰茶叶种植家庭农场 | Red maple cuttage seedling breeding method |
| CN104323240A (en) * | 2014-10-21 | 2015-02-04 | 胡安然 | Full-nutrition formula food for colorectal cancer |
-
2015
- 2015-11-13 CN CN201510778110.5A patent/CN105230433A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1754007A1 (en) * | 1989-12-19 | 1992-08-15 | Н.Ф. Серегин | Method of fruit crop multiplication |
| CN103081683A (en) * | 2013-01-22 | 2013-05-08 | 沈阳农业大学 | Improved American red maple twig cutting propagation method |
| CN103563617A (en) * | 2013-10-12 | 2014-02-12 | 杭州市园林绿化股份有限公司 | Red maple cuttage breeding method |
| CN104322240A (en) * | 2014-09-05 | 2015-02-04 | 郎溪县凌笪乡永辰茶叶种植家庭农场 | Red maple cuttage seedling breeding method |
| CN104323240A (en) * | 2014-10-21 | 2015-02-04 | 胡安然 | Full-nutrition formula food for colorectal cancer |
Non-Patent Citations (4)
| Title |
|---|
| 何素芬等: "美国红枫硬枝扦插育苗试验研究", 《四川林业科技》 * |
| 刘振岩，李震三主编: "《山东果树》", 31 August 2000, 上海：上海科学技术出版社 * |
| 绍兴市全民科学素质工作领导小组办公室等: "《农民适用技术普及读本》", 30 April 2010, 北京：中国农业科学技术出版社 * |
| 谢云著: "《园林苗木生产技术手册》", 31 January 2012, 北京：中国林业出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106416680A (en) * | 2016-09-08 | 2017-02-22 | 广西壮族自治区林业科学研究院 | Melaleuca alternifolia cutting propagation method |
| CN107047203A (en) * | 2017-05-10 | 2017-08-18 | 蚌埠王恒亮盆景艺术有限公司 | A kind of trident maple cuttage seeding cultivating method |
| CN113875409A (en) * | 2021-10-14 | 2022-01-04 | 山东农业大学 | Efficient cutting propagation method for holly |
| CN114793653A (en) * | 2022-04-19 | 2022-07-29 | 广西壮族自治区南宁良凤江国家森林公园 | Litsea cubeba hard branch cutting propagation method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103975778B (en) | A kind of grafting Exocarpium Citri Rubrum kind seedling-growing method | |
| CN104081954B (en) | Paper mulberry cuttage propagation method | |
| CN102227976B (en) | Litsea cubeba pers asexual quick propagation and seedling breeding method | |
| CN103460931A (en) | Erythrophloeum fordii cutting propagation method | |
| CN104585007A (en) | Cuttage seedling culture method of liquidambar styraciflua | |
| CN108605537A (en) | A kind of method of purplefruit holly leaf epicormic branch cutting | |
| CN106034722A (en) | Cutting propagation method of golden leaf plant namely weigela florida variegata | |
| CN105493906A (en) | Orange seedling grafting method | |
| CN100367843C (en) | Walnut twig cutting propagation technology | |
| CN105230433A (en) | Cutting propagation method for American red maples | |
| CN109089687A (en) | Borneol camphor tree cuttage and seedling culture propagation method | |
| CN108718740A (en) | Using mulch to the method for golden Chinese scholartree cuttage and seedling culture | |
| CN113785706B (en) | Cutting seedling method of Siberian apricot trees | |
| CN103907497A (en) | Rapid cutting propagation method of test-tube plum plantlets | |
| CN104126419B (en) | Ginkgo ancient tree choiceness breeding garden method for building up | |
| CN103749126B (en) | The sustainable cultivation method of a kind of root of three-nerved spicebush | |
| CN105519419A (en) | Method for propagating twigs of acer palmatum Jinling Haungfeng by cutting | |
| CN103053312B (en) | Propagation method of Pterocarya stenoptera var. zhijiangensis | |
| CN113412737B (en) | Efficient cutting propagation seedling raising method for celastrus angulatus | |
| CN108157117A (en) | A kind of method for inspiring ginkgo and doing generation again | |
| CN110558130B (en) | Cutting method of cauliflower | |
| CN106962163A (en) | A kind of Chinese rose cottage method | |
| CN108184528B (en) | Method for heel-carrying cuttage of ginkgo biloba | |
| CN105706840A (en) | Method for improving cutting propagation survival rate of clerodendrum trichotomum | |
| CN111183808A (en) | Cuttage propagation method for nitraria twigs |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160113 |
|
| RJ01 | Rejection of invention patent application after publication |