CN114793653A - Litsea cubeba hard branch cutting propagation method - Google Patents
Litsea cubeba hard branch cutting propagation method Download PDFInfo
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- CN114793653A CN114793653A CN202210407314.8A CN202210407314A CN114793653A CN 114793653 A CN114793653 A CN 114793653A CN 202210407314 A CN202210407314 A CN 202210407314A CN 114793653 A CN114793653 A CN 114793653A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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Abstract
The invention provides a cuttage propagation method of litsea cubeba, belonging to the technical field of propagation of litsea cubeba, and the method comprises the following steps: selecting factors which have obvious influence on the cuttage rooting effect of the litsea cubeba to carry out orthogonal test, investigating the rooting condition of the cuttings after 2 months of cuttage, and carrying out statistical analysis on the root system configuration, the growth condition of the new shoots and the photosynthetic property of the litsea cubeba cuttage seedlings after 4 months of cuttage, thereby accurately finding out the optimal combination suitable for the cuttage rooting and the growth of the cuttage seedlings of the litsea cubeba. The method comprehensively screens the method suitable for the cuttage rooting of the litsea cubeba and the growth of the cuttage seedlings from the aspects of rooting conditions, root system configurations, new shoot growth conditions and photosynthetic characteristics, further optimizes a cuttage propagation technical system, and provides a certain theoretical basis and technical support for the popularization of the cuttage work of the litsea cubeba in the future production.
Description
Technical Field
The invention relates to the technical field of Siberian cocklebur vine propagation, in particular to a Siberian cocklebur vine cutting propagation method.
Background
Siberian cocklebur (erythropulum scandens Bl.) also called leech vine, dragon balsam stem, slender periploca vine and the like is a woody liana plant of Siberian cocklebur belonging to the family Cyperaceae, and is mainly distributed in places such as Guangxi, Guangdong, Yunnan, Guizhou and the like in China, and is commonly found in hills or mountain stream sides, valleys, dense forests or sparse forests, forest edges or brush forests. The litsea cubeba has the effects of clearing heat and promoting diuresis, and dispelling wind and activating blood circulation, and is commonly used for treating diseases such as hepatitis, tumor, urethritis, acute nephritis and the like in Guangxi folk. In addition, the litsea cubeba belongs to one of the leafy vegetables in the wild vegetables, has unique flavors of freshness, mellowness, aroma and the like, is rich in carotene, vitamins and mineral substances such as iron, zinc, copper, manganese and the like, and has the habit of picking the litsea cubeba as tea for drinking in Guangxi folk. Meanwhile, the litsea japonica flowers are arranged into axillary bifurcate cymbidium inflorescence, the corolla is white, the top of the calyx tube is provided with reserved wavy split teeth, the color is light reddish brown when the calyx tube is immature, and the color is yellow brown after the calyx tube is dry. The leaf paper is thick paper, oval, green on the leaf and pink green on the back, and the fruit becomes drooping fruit which is bluish purple when ripe, and is the ornamental tree species of leaf and fruit.
The main propagation mode of the existing litsea cubeba is cutting propagation, the cutting propagation has the advantages of simplicity, convenience, capability of keeping excellent properties of the litsea cubeba in nature and the like, but the litsea cubeba belongs to tree species with slow cutting rooting, wound of the litsea cubeba is difficult to form callus, particularly difficult to root under the untreated condition, long time for rooting and difficult to survive. In recent years, the demand of the litsea cubeba seedlings is increased year by year, the supply of the seedling market is not in demand, and the problem of how to rapidly propagate a large number of high-quality litsea cubeba seedlings becomes urgent to solve in production.
Disclosure of Invention
The invention aims to provide a method for cuttage propagation of litsea cubeba, which solves the technical problems mentioned in the background technology.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the cuttage propagation method of the litsea cubeba comprises the following steps: selecting factors which have obvious influence on the cuttage rooting effect of the litsea cubeba to carry out orthogonal test, investigating the rooting condition of the cutting shoot after 2 months of cuttage, and carrying out statistical analysis on the root system configuration, the growth condition of the new shoot and the photosynthetic property of the litsea cubeba cuttage seedling after 4 months of cuttage, thereby accurately finding out the optimal combination suitable for the rooting of the cutting shoot and the growth of the cuttage seedling of the litsea cubeba.
Further, the specific process of the orthogonal test is as follows: adopting three-factor three-level orthogonal experimental design, setting 3 factors of plant growth regulator, concentration and branch position, setting 3 levels of each factor, adopting NAA, IBA and ABT No. 1 as the plant growth regulator, setting the concentrations to be 500mg/L, 1000mg/L and 1500mg/L, setting the type of cutting shoot to be three types of upper, middle and lower according to L9(3) 3 The orthogonal test table was run, with 9 treatments randomly arranged, each treatment setting three replicates, each replicate 30 cuttings, regardless of the interaction between factors.
Further, the specific process of investigating the rooting condition of the cutting shoot comprises the following steps: after 60d of cutting, the seedlings are taken out from the whole garden to investigate the rooting condition, the rooting rate of each treatment is investigated and counted, the root length is more than or equal to 1mm and is regarded as the root, 10 cuttings are randomly extracted from each treatment combination, the rooting number of each cutting is counted and the length is measured by a ruler, the root length is accurate to 0.1cm, the average value is taken, the average root number, the average root length, the longest root length, the number of roots with the length more than 5cm and the rhizosphere effect index are obtained, the rooting rate is the number of rooting cuttings/the total cuttings multiplied by 100%, and the root system effect index is the number of rooting cuttings multiplied by the average root length/the total cuttings multiplied by 100%.
Further, the specific process of the seedling root system configuration determination is as follows: after 4 months of cutting, 10 cutting shoots are randomly extracted from each treatment, scanned by a root system scanner, and the indexes of the total root length, the total root surface area, the total projection area, the root system diameter, the total volume, the root tip number and the like are measured, and then analyzed by adopting WinRHIO software.
Further, the specific process of the young sprout growth condition investigation comprises the following steps:
observing and counting the number of young shoot branches of each treated cutting shoot by naked eyes, calculating the young shoot rate, randomly extracting 10 cutting shoots in each treatment combination, measuring the length of the young shoots by using a ruler to be accurate to 0.1cm, measuring the basal diameter of the young shoots by using a digital display vernier caliper to be accurate to 0.01mm, measuring the leaf area by using an LI-3000C leaf area instrument, and observing the index of the number of the leaves.
Further, the specific process of determining the photosynthetic property of the cutting seedling leaves comprises the following steps: after 4 months of cuttage, measuring the photosynthetic characteristics of leaves, wherein an acetone-ethanol method is adopted for measuring the chlorophyll content, and a photosynthetic parameter measuring method comprises the following steps: the indexes of net photosynthetic rate, stomatal conductance, intercellular carbon dioxide concentration and transpiration rate are selected from 9:00-11:30, measuring, namely selecting healthy plants with basically consistent growth vigor in each treatment, selecting 3 mature functional leaves with the same leaf age and leaf position from each plant, measuring by using a combiner, and repeatedly measuring each leaf for three times by using a natural light source.
Due to the adoption of the technical scheme, the invention has the following beneficial effects:
the method can accurately find out the optimal combination suitable for the rooting of the cuttage slips of the litsea cubeba and the growth of the cuttage seedlings, further optimize a cuttage propagation technical system, provide certain theoretical basis and technical support for the popularization of the cuttage work of the litsea cubeba in future production, and carry out statistical analysis through the rooting, the root system configuration, the growth condition of new shoots and the photosynthetic property to select the seedling bodies with better growth and better photosynthesis.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below with reference to preferred embodiments. It should be noted, however, that the numerous details set forth in the description are merely for the purpose of providing the reader with a thorough understanding of one or more aspects of the present invention, which may be practiced without these specific details.
The cuttage propagation method of the litsea cubeba comprises the following steps:
step 1: test design and method:
adopting three-factor three-level orthogonal experimental design, setting 3 factors of plant growth regulator, concentration and branch position, setting 3 levels of each factor, adopting NAA, IBA and ABT1 numbers as plant growth regulator, setting the concentrations to be 500mg/L, 1000mg/L and 1500mg/L, setting the type of cutting shoot to be three types, namely upper, middle and lower, according to L9(3) 3 The orthogonal test table was run, with 9 treatments randomly arranged, each treatment setting three replicates, each replicate 30 cuttings, regardless of the interaction between factors.
TABLE 1 Litsea cubeba hard wood cuttage orthogonal test factors and levels
TABLE 2 Litsea cubeba hard wood cutting orthogonal test table
Step 2: investigation of rooting
After 60d of cutting, the seedlings are taken out from the whole garden to investigate the rooting condition, the rooting rate of each treatment is investigated and counted, the root length is more than or equal to 1mm and is regarded as the root, 10 cuttings are randomly extracted from each treatment combination, the rooting number of each cutting is counted and the length is measured by a ruler, the root length is accurate to 0.1cm, the average value is taken, the average root number, the average root length, the longest root length, the number of roots with the length more than 5cm and the rhizosphere effect index are obtained, the rooting rate is the number of rooting cuttings/the total cuttings multiplied by 100%, and the root system effect index is the number of rooting cuttings multiplied by the average root length/the total cuttings multiplied by 100%.
And step 3: root morphology index determination
After 4 months of cutting, 10 cuttings from each treatment were randomly picked, scanned with a root system scanner (Epson Perfection V700Photo, japan), and analyzed with WinRHIZO software. The measurements included total root length (cm), total root surface area (cm) 2 ) The total root projection area (cm2), root diameter (mm) and total volume (cm) 3 ) And the number of root tips (strips) and other indexes of the root system.
And 4, step 4: investigation of shoot growth
And (3) carrying out young shoot growth condition investigation 4 months after cutting, observing the number of young shoot branches of each treated cutting shoot by naked eyes, calculating the young shoot rate, randomly drawing 10 cutting shoots in each treatment combination, measuring the length of the young shoots by adopting a ruler to be accurate to 0.1cm, measuring the basal diameter of the young shoots by adopting a digital display vernier caliper to be accurate to 0.01mm, measuring the leaf area by adopting an LI-3000C leaf area meter, and observing the index of the leaf number.
And 5: research on photosynthetic Properties
After 4 months of cuttage, measuring the photosynthetic property of the leaves, and measuring the chlorophyll content of the leaves by an acetone-ethanol method; the photosynthetic parameter determination method comprises the following steps: indexes such as net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular carbon dioxide concentration (Ci) and transpiration rate (Tr) are selected in the morning of clear weather 9:00-11:30, selecting healthy plants with basically consistent growth vigor in each treatment, selecting mature functional leaves with the same leaf position and 3 leaf ages for each plant, measuring by using LI-6400XT, repeating the measurement for three times for each leaf, and using a natural light source for measurement.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.
Claims (6)
1. The cuttage propagation method of the litsea cubeba is characterized by comprising the following steps: selecting factors which have obvious influence on the rooting effect of the cuttage branches of the litsea cubeba to carry out orthogonal test, investigating the rooting condition of the cuttings after 2 months of cuttage, and carrying out statistical analysis on the root system configuration, the growth condition of new shoots and the photosynthetic property of the cuttage seedlings of the litsea cubeba after 4 months of cuttage, thereby accurately finding out the optimal combination suitable for the rooting of the cuttings of the litsea cubeba and the growth of the cuttage seedlings.
2. The cuttage propagation method of the litsea cubeba as claimed in claim 1, characterized in that: the specific process of the orthogonal test is as follows: adopting three-factor three-level orthogonal experimental design, setting 3 factors of plant growth regulator, concentration and branch position, setting 3 levels of each factor, adopting NAA, IBA and ABT No. 1 as the plant growth regulator, setting the concentrations to be 500mg/L, 1000mg/L and 1500mg/L, setting the type of cutting shoot to be three types of upper, middle and lower according to L9(3) 3 The orthogonal test table was run, with 9 treatments randomly arranged, each treatment setting three replicates, each replicate 30 cuttings, regardless of the interaction between factors.
3. The cuttage propagation method of the litsea cubeba as claimed in claim 1, characterized in that: the specific process of investigating the rooting condition of the cutting shoot comprises the following steps: after 60d of cutting, the seedlings are taken out from the whole garden to investigate the rooting condition, the rooting rate of each treatment is investigated and counted, the root length is more than or equal to 1mm and is regarded as the root, 10 cuttings are randomly extracted from each treatment combination, the rooting number of each cutting is counted and the length is measured by a ruler, the root length is accurate to 0.1cm, the average value is taken, the average root number, the average root length, the longest root length, the number of roots with the length more than 5cm and the rhizosphere effect index are obtained, the rooting rate is the number of rooting cuttings/the total cuttings multiplied by 100%, and the root system effect index is the number of rooting cuttings multiplied by the average root length/the total cuttings multiplied by 100%.
4. The method for cuttage propagation of litsea cubeba as claimed in claim 1, wherein the specific process of the root system configuration determination of the cuttage seedlings is as follows: after 4 months of cutting, 10 cutting shoots are randomly extracted from each treatment, scanned by a root system scanner, and the indexes of the total root length, the total root surface area, the total projection area, the root system diameter, the total volume, the root tip number and the like are measured, and then analyzed by adopting WinRHIO software.
5. The cuttage propagation method of the litsea cubeba as claimed in claim 1, characterized in that:
and (3) carrying out young shoot growth condition investigation 4 months after the cutting, observing the number of the young shoot branches of each treated cutting and calculating the young shoot rate by naked eyes, randomly drawing 10 cutting in each treatment combination, measuring the length of the young shoot by adopting a ruler to be accurate to 0.1cm, measuring the basal diameter of the young shoot by adopting a digital display vernier caliper to be accurate to 0.01mm, measuring the leaf area by adopting an LI-3000C leaf area meter, and observing the index of the leaf number.
6. The cuttage propagation method of the litsea cubeba as claimed in claim 1, characterized in that: the method comprises the following specific steps of measuring the photosynthetic property of the leaves of the cutting seedlings: after 4 months of cuttage, measuring the photosynthetic characteristics of leaves, wherein an acetone-ethanol method is adopted for measuring the chlorophyll content, and a photosynthetic parameter measuring method comprises the following steps: the indexes of net photosynthetic rate, stomatal conductance, intercellular carbon dioxide concentration and transpiration rate are measured at 9:00-11:30 in the morning of clear weather, plants with basically consistent and healthy growth vigor in each treatment are selected, mature functional leaves with the same leaf age and leaf position of 3 leaves are selected from each plant, the measurement is carried out by a photosynthesizer, each leaf is repeatedly measured for three times, and a natural light source is used for measurement.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105230433A (en) * | 2015-11-13 | 2016-01-13 | 南京林业大学 | Cutting propagation method for American red maples |
| CN109757232A (en) * | 2019-03-14 | 2019-05-17 | 中国医学科学院药用植物研究所云南分所 | A kind of method and largeleaf gambirplant branchlet cuttage seeding of largeleaf gambirplant branchlet cutting propagation |
| CN112840878A (en) * | 2019-11-26 | 2021-05-28 | 国家林业和草原局桉树研究开发中心 | Method for promoting cuttage rooting of rose apple by changing types, concentrations and soaking time of hormones |
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- 2022-04-19 CN CN202210407314.8A patent/CN114793653A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105230433A (en) * | 2015-11-13 | 2016-01-13 | 南京林业大学 | Cutting propagation method for American red maples |
| CN109757232A (en) * | 2019-03-14 | 2019-05-17 | 中国医学科学院药用植物研究所云南分所 | A kind of method and largeleaf gambirplant branchlet cuttage seeding of largeleaf gambirplant branchlet cutting propagation |
| CN112840878A (en) * | 2019-11-26 | 2021-05-28 | 国家林业和草原局桉树研究开发中心 | Method for promoting cuttage rooting of rose apple by changing types, concentrations and soaking time of hormones |
Non-Patent Citations (4)
| Title |
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| 张玲: "夹竹桃穴盘扦插育苗关键技术的研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》, no. 7, pages 048 - 118 * |
| 张玲;汪天;王华;叶辉;: "花叶夹竹桃穴盘扦插繁殖技术研究", 亚热带植物科学, no. 02, pages 43 - 47 * |
| 符策等: "不同外源激素、基质处理对赤苍藤扦插生根的影响", 南方农业, no. 05, pages 139 - 140 * |
| 韦雪英;符策;冯兰;施丁寿;: "赤苍藤扦插繁育技术", 中国热带农业, no. 05, pages 69 - 71 * |
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