CN111183808A - Cuttage propagation method for nitraria twigs - Google Patents

Cuttage propagation method for nitraria twigs Download PDF

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Publication number
CN111183808A
CN111183808A CN202010074305.2A CN202010074305A CN111183808A CN 111183808 A CN111183808 A CN 111183808A CN 202010074305 A CN202010074305 A CN 202010074305A CN 111183808 A CN111183808 A CN 111183808A
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cutting
soil
matrix
seedlings
transplanting
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赵丽丽
孙小富
龙才
黄莉娟
赵鑫
王雷挺
陈可可
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Guizhou University
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Guizhou University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00—Vegetative propagation
    • A01G2/10—Vegetative propagation by means of cuttings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005—Cultivation methods

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention relates to a cuttage propagation method for the twig of nitraria tangutorum bobr, which comprises the following specific steps: 1) preparing a substrate; 2) obtaining cutting slips; 3) respectively soaking the cutting strips obtained in the step 2) in indolebutyric acid (IBA), naphthylacetic acid (NAA) and rooting powder (ABT1, containing 30% IAA and 20% NAA); 4) cutting twigs; 5) managing after the insertion; 6) and (5) transplanting and planting. The method is simple and easy to implement, can realize the cuttage and rapid propagation of the nitraria tangutorum bobr within a short time, has the cuttage survival rate of 75.00 percent, and provides an effective method for the nitraria tangutorum bobr in aspects of vegetation recovery, water and soil conservation ecological benefits, forage grass development and the like.

Description

Cuttage propagation method for nitraria twigs
Technical Field
The invention belongs to the technical field of plant propagation, and particularly relates to a cuttage propagation method for a nitraria davidii twigs.
Background
The nitraria davidii (Sophora davidii) is also called wolf tooth thorn and bitter thorn, belongs to the Sophora of subfamily of Leguminosae and is provided with thorn shrubs for many years, is widely distributed in northwest, north China, east China and southwest areas, has wide ecological adaptability, has the outstanding characteristics of drought resistance, barren resistance, fire burning resistance, trampling resistance and the like, has deep root and strong sprouting capability and has strong biological nitrogen fixation capability, and is a pioneer tree species for water and soil conservation, vegetation restoration, soil improvement and afforestation in karst mountainous areas and arid and semi-arid areas. The leaves and stems of the feed are tender and good in palatability, the seeds and the flowers also contain rich vitamins, mineral elements and amino acids, the flowering period is long, the honey powder is rich, and the nitraria flowers are high-quality forage grass and good honey source plants in production. The nitraria sibirica pall is a cross-pollinated plant, the excellent characters of the excellent germplasm can be well fixed through cutting propagation, the excellent germplasm can be rapidly propagated, and the method is an effective measure for accelerating the popularization and the application of the nitraria sibirica pall. Therefore, the research and exploration of the cuttage and rapid propagation technology of the nitraria tangutorum bobr has important significance for water and soil conservation, ecological restoration and forage development.
CN 201710964389.5A cuttage propagation method of Ardisia obovata, which discloses a cuttage propagation method of Ardisia obovata, comprising the following steps: firstly, manufacturing a field bell cutting spike; step two, placing the base part of the cutting into a rooting inducer, soaking for 6 hours, taking out, and draining in the shade until the surface is anhydrous; and step three, cutting the cuttings treated in the step two into a seedbed paved with a matrix.
This invention (CN201710964389.5) has problems: 1. the cutting slips are soaked in a rooting inducer for 6 hours, and the soaking time is too long, so that the propagation cycle of the nitraria tangutorum bobr is influenced; 2. before the cutting is placed in the rooting inducer, the base part of the cutting is placed in 800 times of carbendazim wettable powder to be soaked for 0.5h, so that the cutting of the nitraria tangutorum is easy to be necrotic, and the rooting rate is reduced; 3. the matrix consists of peat soil, vermiculite and fine river sand according to the volume ratio of 2:1:1, and the fine river sand is easy to cause the nitraria tangutorum slip to be difficult to root and slow to grow.
CN 201410841544.0A cuttage propagation method of anisetree bark discloses a cuttage propagation method of anisetree bark, which comprises the following steps: a) selection and treatment of cutting shoots: selecting a robust branch, cutting the robust branch into a cutting, soaking the base of the cutting in a disinfectant for 0.5h, and then soaking in a rooting inducer for 2 h; b) preparation of the substrate and the seedbed: the cutting medium is a uniform mixture of peat soil, perlite, vermiculite and fine river sand, and is made into a seedbed with the thickness of 15cm after being disinfected by disinfectant; c) cuttage: obliquely cutting the cutting slips in the seedbed matrix to a depth of 7-10 cm; d) managing the seedbed after cuttage: spraying water to the seedbed in the morning and evening every day, spraying 500 times of thiophanate methyl after 7 days, spraying twice every week, spraying 3/4MS nutrient solution after one week, and culturing to root and sprout.
The problem with this invention (CN201410841544.0) is: 1. the cuttage is firstly soaked in 0.3 percent potassium permanganate solution for 0.5h and then fished out, and then is placed in a rooting inducer (100-300 mg.L)-150-200 mg.L of naphthylacetic acid-1200-80 mg.L of gibberellin-110-50 mg.L of indolebutyric acid-1100-500 mg.L of ABT1 rooting powder-1Chlormequat chloride and 50-100 mg.L-1The vitamin C) is soaked for 2 hours and then fished out; the growth of the cutting slips of the nitraria tangutorum bobr is easy to slow, the survival rate is low, and the nitraria tangutorum bobr is not easy to root, and 2, the matrix of the nitraria tangutorum bobr consists of peat soil, vermiculite and fine river sand according to the volume ratio of 2:1: 1; the fine river sand easily causes the nitraria tangutorum bobr cutting to be difficult to root and slow in growth.
DOI 10.13456/j. cnki. lykt.2004.05.013 Wang Hui Ru, Guoxing; the nitraria tangutorum seedling raising technology discloses a nitraria tangutorum seed seedling raising method which comprises the following steps: 1. a seed collection method; 2. selecting a nursery land; 3. preparing soil and preparing nutrient soil; 4. accelerating germination of seeds; 5. sowing; 6. and (5) seedling stage management. The method is suitable for propagating the nitraria tangutorum bobr, and has complex steps and long period.
In order to overcome the problems, the inventor invents a method which is simple and easy to implement, can realize the cuttage and rapid propagation of the nitraria tangutorum bobr in a short time through a large number of research experiments, has the cuttage survival rate of 75.00 percent, and provides an effective method for the nitraria tangutorum bobr in the aspects of vegetation recovery, water and soil conservation ecological benefits, forage development and the like.
Disclosure of Invention
The invention aims to provide a cuttage propagation method for the twig of nitraria tangutorum bobr.
The invention relates to a nitraria tangutorum twig cutting propagation method, which comprises the following steps:
1) selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing 70-80% of peat soil and 20-30% of vermiculite, spraying 60-100% of a wettable carbendazim powder 800-1000 times of solution on the mixed substrate to sterilize the cutting substrate, and covering with a mulching film for 2-4 days after thorough watering for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting, and cutting the cutting into cutting strips with the length of 5-9 cm;
3) respectively soaking 2-5 cm parts of the lower ends of the cutting strips obtained in the step 2) in exogenous hormones for 0.5-2 hours, wherein the exogenous hormones are one of indolebutyric acid (IBA), naphthylacetic acid (NAA) and rooting powder (ABT1, 20-40% of IAA and 10-30% of NAA), and the concentration of each exogenous hormone is 100-400 mg.L-1；
4) Cuttage: punching the disinfected matrix soil by using a stick to a depth of 2-5 cm, then placing the cutting strips into the holes, tightly pressing the matrix soil by using hands to enable the cutting strips to be in close contact with the matrix soil, and keeping the plant-row spacing at 5-9 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting, a sunshade net with the light transmittance of 50-70% is covered on the shed top, the situation that the leaves of the cutting slips are excessively transpired and the cutting slips are dehydrated and dried up is prevented, and watering, fertilizing and weeding are carried out timely;
6) transplanting and planting: and (3) checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into holes with the depth of 8-12 cm during transplanting, putting the seedlings into the centers of the holes during transplanting to enable root systems to be spread, filling fine soil on surface layers into the holes to remove impurities, keeping the row spacing of the seedlings at 1.2m multiplied by 1.5m during transplanting, and planting 350-400 plants per mu.
Preferably, the first and second liquid crystal materials are,
1) selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing 73-78% of peat soil and 23-27% of vermiculite, spraying 70-90% of wettable carbendazim powder 850-950 times of solution on the mixed substrate to sterilize, and covering with a mulching film for 2.5-3.5 days after thorough watering for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting, and cutting the cutting into cutting strips with the length of 6-8 cm;
3) respectively soaking 3-4 cm parts of the lower ends of the cutting strips in the step 2) in exogenous hormones for 0.8-1.8 h, wherein the exogenous hormones are one of indolebutyric acid (IBA), naphthylacetic acid (NAA) and rooting powder (ABT1, containing 25-35% of IAA and 15-25% of NAA), and the concentration of each exogenous hormone is 200-300 mg.L < -1 >;
4) cuttage: punching the disinfected matrix soil by using a stick to a depth of 3-4 cm, then placing the cutting strips into the holes and tightly pressing the matrix soil by using hands to ensure that the cutting strips are tightly contacted with the matrix soil, and keeping the plant-row spacing at 6-8 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting, a sunshade net with the light transmittance of 55-65% is covered on the shed top, the situation that the leaves of the cutting slips are excessively transpired and the cutting slips are dehydrated and dried up is prevented, and watering, fertilizing and weeding are carried out timely;
6) transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into holes with the depth of 9-11 cm during transplanting, putting the seedlings into the centers of the holes during transplanting to enable the root systems to be spread, filling fine soil on surface layers into the holes to remove impurities, keeping the row spacing of the seedlings at 1.2m multiplied by 1.5m during transplanting, and planting 360-390 plants per mu.
It is further preferred that the first and second liquid crystal compositions,
1) selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing 75% peat soil and 25% vermiculite, spraying 80% wettable carbendazim powder 900 times solution on the mixed substrate to sterilize, and covering with mulching film for 3 days after thorough watering for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting, and cutting the cutting into cutting strips with the length of 7 cm;
3) respectively soaking 3.5cm parts of the lower ends of the cutting strips in the step 2) in exogenous hormone for 2h, wherein the exogenous hormone is one of indolebutyric acid (IBA), naphthylacetic acid (NAA) and rooting powder (ABT1, containing 30% IAA and 20% NAA), and the concentrations of the exogenous hormone and the rooting powder are respectively 100 mg.L < -1 >;
4) cuttage: punching the disinfected matrix soil by using a stick to make the depth of the hole be 3.5cm, then placing the cutting strip into the hole and tightly pressing the matrix soil by using hands to make the cutting strip tightly contact with the matrix soil, and keeping the plant-row spacing at 7 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting board, a sunshade net with the light transmittance of 60 percent is covered on the shed roof, the situation that the leaves of the cutting slips are excessively transpired and are dehydrated and dried up is prevented, and watering, weeding and fertilizing are carried out timely;
6) transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into 10 cm-deep hole pits during transplanting, putting the seedlings into the center of the hole pits during planting to enable the root systems to be spread, filling surface-layer fine soil into the holes to remove impurities, keeping the row spacing of the seedlings at 1.2m multiplied by 1.5m during transplanting, and planting 370 plants per mu.
According to the propagation method, when the cutting slips in the step 2) are cut, a flat edge is adopted at the upper end, an inclined edge is adopted at the lower end, and the upper edge and the lower edge are sharp and smooth. Removing leaves with the base part being less than 2-5 cm, and reserving the leaves with the base part being more than 2-5 cm.
Preferably, the first and second liquid crystal materials are,
according to the propagation method, when the cutting slips in the step 2) are cut, a flat edge is adopted at the upper end, an inclined edge is adopted at the lower end, and the upper edge and the lower edge are sharp and smooth. Removing the leaves with the base part being less than 3-4 cm, and reserving the leaves with the base part being more than 3-4 cm.
It is further preferred that the first and second liquid crystal compositions,
according to the propagation method, when the cutting slips in the step 2) are cut, a flat knife edge is adopted at the upper end, an inclined knife edge is adopted at the lower end, the upper knife edge and the lower knife edge are sharp and smooth, the blades with the base part being less than 3.5cm are removed, and the blades with the base part being more than 3.5cm are reserved.
According to the propagation method, timely watering is carried out after cuttage in the management after cuttage in the step 5), namely that the water content of the matrix soil is kept between 50 and 70 percent, and the air humidity is controlled to be more than 85 percent.
Preferably, the first and second liquid crystal materials are,
according to the propagation method, timely watering is carried out after cuttage in the management after cuttage in the step 5), namely that the water content of the matrix soil is kept between 55 and 65 percent, and the air humidity is controlled to be more than 85 percent.
It is further preferred that the first and second liquid crystal compositions,
according to the propagation method, timely watering is carried out after cuttage in the management after cuttage in the step 5), namely that the water content of the matrix soil is kept at 60%, and the air humidity is controlled to be more than 85%.
According to the propagation method, the timely weeding and fertilizing in the management after the cuttings in the step 5) are that 2% of monopotassium phosphate and 0.8% of urea are sprayed on the leaf surfaces after the cuttings root and grow leaves.
The invention has the advantages of
1. The invention researches the cutting propagation of the nitraria tangutorum bobr for the first time, the obtained nitraria tangutorum bobr has high cutting rooting rate, high propagation speed, strong seedlings, low cost and simple and convenient operation, provides an effective method for the nitraria tangutorum bobr in aspects of vegetation recovery, water and soil conservation ecological benefits, forage grass development and the like, and has stronger practicability and popularization value.
2. The cutting medium is sprayed and disinfected by using 800-1000 times of 80% wettable carbendazim powder solution, and the cutting medium is covered with a mulching film for 3 days for later use after being thoroughly watered, so that the disinfection effect is more complete, the robust seedlings of the nitraria tangutorum bobr are ensured, the disease resistance is strong, the pathological changes are prevented, the rooting speed and the rooting quality are improved, and the cutting survival rate is improved.
3. The film arched shed built on the seedbed can provide a heat-preservation and moisture-retention environment for the nursery stock, so that direct sunlight is avoided, the water evaporation of the nursery stock is reduced, and the cuttage survival rate is improved.
4. After the scion roots and leaves grow, low-concentration monopotassium phosphate and urea are sprayed on the leaf surfaces to supplement nutrition and weeding effects required by the nitraria tangutorum bobr seedlings, so that the seedlings can grow in a better environment.
5. The cuttage survival rate experiment of the cichorium victorialis tender flower by different exogenous hormone treatments proves that the exogenous hormone indolebutyric acid (IBA) treatment is optimal, and the concentration of the indolebutyric acid (IBA) is 100 mg.L-1The soaking time of the cutting slips is 2 hours, the cutting rooting rate is better, the rooting rate reaches 75.00%, and the root system effect index is 3.78.
Detailed Description
1) Example 1
2) Selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing 75% peat soil and 25% vermiculite, spraying 80% wettable carbendazim powder 900 times solution on the mixed substrate to sterilize, and covering with mulching film for 3 days after thorough watering for later use;
3) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting, shearing the cutting into cutting strips with the length of 7cm, wherein when the cutting is conducted, the upper end adopts a flat edge, the lower end adopts an inclined edge, and the upper edge and the lower edge are sharp and smooth. The leaf below 3.5cm from the base was removed and the leaf above 3.5cm was retained.
4) Treating the lower end of the cutting strip for 3.5cm at a concentration of 100 mg.L-1The exogenous hormone indolebutyric acid is soaked for 2 hours.
4) Cuttage: punching the disinfected matrix soil by using a stick to make the depth of the hole be 3.5cm, then placing the cutting strip into the hole and tightly pressing the matrix soil by using hands to make the cutting strip tightly contact with the matrix soil, and keeping the plant-row spacing at 7 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting board, a sunshade net with the light transmittance of 60 percent is covered on the shed roof, the situation that the leaves of the cutting slips are excessively transpired and are dehydrated and dried up is prevented, and watering, fertilizing and weeding are carried out timely; the water content of the matrix soil is kept at 60%, and the air humidity is controlled to be above 85%. After the scions take roots and grow leaves, 2 percent of monopotassium phosphate and 0.8 percent of urea are sprayed on the leaves.
6) Transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into 10 cm-deep hole pits during transplanting, putting the seedlings into the center of the hole pits during planting to enable the root systems to be spread, filling surface-layer fine soil into the holes to remove impurities, keeping the row spacing of the seedlings at 1.2m multiplied by 1.5m during transplanting, and planting 370 plants per mu.
Example 2
1) Selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing 75% peat soil and 25% vermiculite, spraying 80% wettable carbendazim powder 900 times solution on the mixed substrate to sterilize, and covering with mulching film for 3 days after thorough watering for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting, shearing the cutting into cutting strips with the length of 7cm, wherein when the cutting is conducted, the upper end adopts a flat edge, the lower end adopts an inclined edge, and the upper edge and the lower edge are sharp and smooth. The leaf below 3.5cm from the base was removed and the leaf above 3.5cm was retained.
3) Treating the lower end of the cutting strip for 3.5cm at a concentration of 100 mg.L-1The exogenous hormone naphthylacetic acid is soaked for 2 hours.
4) Cuttage: and (3) punching the disinfected matrix soil by using a stick to make the depth of the hole be 3.5cm, then placing the cutting strip into the hole, and tightly pressing the matrix soil by using hands to make the cutting strip tightly contact with the matrix soil, wherein the plant-row spacing is kept at 7 cm.
5) Managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting board, a sunshade net with the light transmittance of 60 percent is covered on the shed roof, the situation that the leaves of the cutting slips are excessively transpired and are dehydrated and dried up is prevented, and watering, fertilizing and weeding are carried out timely; the water content of the matrix soil is kept at 60%, and the air humidity is controlled to be above 85%. After the scions take roots and grow leaves, 2 percent of monopotassium phosphate and 0.8 percent of urea are sprayed on the leaves.
6) Transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into 10 cm-deep hole pits during transplanting, putting the seedlings into the center of the hole pits during planting to enable the root systems to be spread, filling surface-layer fine soil into the holes to remove impurities, keeping the row spacing of the seedlings at 1.2m multiplied by 1.5m during transplanting, and planting 370 plants per mu.
Example 3
1) Selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing 75% peat soil and 25% vermiculite, spraying 80% wettable carbendazim powder 900 times solution on the mixed substrate to sterilize, and covering with mulching film for 3 days after thorough watering for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting, shearing the cutting into cutting strips with the length of 7cm, wherein when the cutting is conducted, the upper end adopts a flat edge, the lower end adopts an inclined edge, and the upper edge and the lower edge are sharp and smooth. The leaf below 3.5cm from the base was removed and the leaf above 3.5cm was retained.
3) Treating the lower end of the cutting strip for 3.5cm at a concentration of 100 mg.L-1The exogenous hormone rooting powder (containing 30% IAA and 20% NAA) is soaked for 2 h.
4) Cuttage: and (3) punching the disinfected matrix soil by using a stick to make the depth of the hole be 3.5cm, then placing the cutting strip into the hole, and tightly pressing the matrix soil by using hands to make the cutting strip tightly contact with the matrix soil, wherein the plant-row spacing is kept at 7 cm.
5) Managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting board, a sunshade net with the light transmittance of 60 percent is covered on the shed roof, the situation that the leaves of the cutting slips are excessively transpired and are dehydrated and dried up is prevented, and watering, fertilizing and weeding are carried out timely; the water content of the matrix soil is kept at 60%, and the air humidity is controlled to be above 85%. After the scions take roots and grow leaves, 2 percent of monopotassium phosphate and 0.8 percent of urea are sprayed on the leaves.
6) Transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into 10 cm-deep hole pits during transplanting, putting the seedlings into the center of the hole pits during planting to enable the root systems to be spread, filling surface-layer fine soil into the holes to remove impurities, keeping the row spacing of the seedlings at 1.2m multiplied by 1.5m during transplanting, and planting 370 plants per mu.
Example 4
1) Selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing the cutting substrate by 80% peat soil and 20% vermiculite, spraying the mixed substrate with 1000 times solution of 100% wettable carbendazim powder for sterilizing, and covering with mulching film for 4 days after thorough watering for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting slip, shearing the cutting slip into cutting strips with the length of 9cm, wherein when the cutting slip is sheared, the upper end adopts a flat edge, the lower end adopts an inclined edge, and the upper edge and the lower edge are sharp and smooth. The leaf below 5cm from the base was removed and the leaf above 5cm was retained.
3) Respectively placing the 5cm parts at the lower ends of the cutting strips at the concentration of 400 mg.L-1The exogenous hormone indolebutyric acid is soaked for 0.7 h.
4) Cuttage: punching the sterilized matrix soil with a stick to a depth of 5cm, putting the cutting strips into the holes, and tightly pressing the matrix soil with hands to ensure that the cutting strips are tightly contacted with the matrix soil, and keeping the plant-row spacing at 9 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting, a sunshade net with the light transmittance of 70 percent is covered on the shed top, the situation that the leaves of the cutting slips are excessively transpired and dehydrated and dried up is prevented, watering is carried out at proper time, and fertilization and weeding are carried out in time; the water content of the matrix soil is kept at 70%, and the air humidity is controlled to be above 85%. After the scions take roots and grow leaves, 2 percent of monopotassium phosphate and 0.8 percent of urea are sprayed on the leaves.
6) Transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into holes with the depth of 12cm during transplanting, putting the seedlings into the centers of the holes during planting to enable the root systems to be spread, filling surface-layer fine soil into the holes to remove impurities, filling half of the soil, properly pressing the soil by hands to enable the root systems to be in close contact with the soil, continuously filling the soil, then stepping on the soil by feet, keeping the row spacing of the plants to be 1.2m multiplied by 1.5m during transplanting, and planting 380 plants per mu.
Example 5
1) Selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing the cutting substrate by 80% peat soil and 20% vermiculite, spraying the mixed substrate with 1000 times solution of 100% wettable carbendazim powder for sterilizing, and covering with mulching film for 4 days after thorough watering for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting slip, shearing the cutting slip into cutting strips with the length of 9cm, wherein when the cutting slip is sheared, the upper end adopts a flat edge, the lower end adopts an inclined edge, and the upper edge and the lower edge are sharp and smooth. The leaf below 5cm from the base was removed and the leaf above 5cm was retained.
3) Respectively placing the 5cm parts at the lower ends of the cutting strips at the concentration of 400 mg.L-1The exogenous hormone naphthylacetic acid is soaked for 0.7 h.
4) Cuttage: punching the sterilized matrix soil with a stick to a depth of 5cm, putting the cutting strips into the holes, and tightly pressing the matrix soil with hands to ensure that the cutting strips are tightly contacted with the matrix soil, and keeping the plant-row spacing at 9 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting, a sunshade net with the light transmittance of 70 percent is covered on the shed top, the situation that the leaves of the cutting slips are excessively transpired and dehydrated and dried up is prevented, watering is carried out at proper time, and fertilization and weeding are carried out in time; the water content of the matrix soil is kept at 70%, and the air humidity is controlled to be above 85%. After the scions take roots and grow leaves, 2 percent of monopotassium phosphate and 0.8 percent of urea are sprayed on the leaves.
6) Transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into holes with the depth of 12cm during transplanting, putting the seedlings into the centers of the holes during planting to enable the root systems to be spread, filling surface-layer fine soil into the holes to remove impurities, filling half of the soil, properly pressing the soil by hands to enable the root systems to be in close contact with the soil, continuously filling the soil, then stepping on the soil by feet, keeping the row spacing of the plants to be 1.2m multiplied by 1.5m during transplanting, and planting 380 plants per mu.
Example 6
1) Selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing the cutting substrate by 80% peat soil and 20% vermiculite, spraying the mixed substrate with 1000 times solution of 100% wettable carbendazim powder for sterilizing, and covering with mulching film for 4 days after thorough watering for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting slip, shearing the cutting slip into cutting strips with the length of 9cm, wherein when the cutting slip is sheared, the upper end adopts a flat edge, the lower end adopts an inclined edge, and the upper edge and the lower edge are sharp and smooth. The leaf below 5cm from the base was removed and the leaf above 5cm was retained.
3) The parts 5cm below the cutting strips are respectively soaked in exogenous hormone rooting powder (containing 20% IAA and 10% NAA) with the concentration of 400 mg.L < -1 > for 0.7 h.
4) Cuttage: punching the sterilized matrix soil with a stick to a depth of 5cm, putting the cutting strips into the holes, and tightly pressing the matrix soil with hands to ensure that the cutting strips are tightly contacted with the matrix soil, and keeping the plant-row spacing at 9 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting, a sunshade net with the light transmittance of 70 percent is covered on the shed top, the situation that the leaves of the cutting slips are excessively transpired and dehydrated and dried up is prevented, watering is carried out at proper time, and fertilization and weeding are carried out in time; the water content of the matrix soil is kept at 70%, and the air humidity is controlled to be above 85%. After the scions root and grow leaves, spraying low-concentration potassium dihydrogen phosphate and urea on the leaf surfaces.
6) Transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into holes with the depth of 12cm during transplanting, putting the seedlings into the centers of the holes during planting to enable the root systems to be spread, filling surface-layer fine soil into the holes to remove impurities, filling half of the soil, properly pressing the soil by hands to enable the root systems to be in close contact with the soil, continuously filling the soil, then stepping on the soil by feet, keeping the row spacing of the plants to be 1.2m multiplied by 1.5m during transplanting, and planting 380 plants per mu.
Example 7
1) Selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing 73% peat soil and 27% vermiculite, spraying the mixed substrate with 850 times of 65% wettable carbendazim powder solution to disinfect the cutting substrate, and covering with mulching film for 2 days after the substrate is thoroughly watered for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting, shearing the cutting into 6 cm-long cutting slips, wherein when the cutting is sheared, the upper end adopts a flat edge, the lower end adopts an inclined edge, and the upper edge and the lower edge are sharp and smooth. The leaf below 3cm from the base was removed and the leaf above 3cm was retained.
3) Respectively placing 2.5cm parts of the lower ends of the cutting strips at the concentration of 150 mg.L-1The exogenous hormone indolebutyric acid is soaked for 1 h.
4) Cuttage: punching the sterilized matrix soil with a stick to a depth of 3cm, putting the cutting strips into the holes, and tightly pressing the matrix soil with hands to ensure that the cutting strips are tightly contacted with the matrix soil, and keeping the plant-row spacing at 6 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting, a sunshade net with 55% of light transmittance is covered on the shed top, the situation that the leaves of the cutting slips are excessively transpired, the cutting slips are dehydrated and dried up is prevented, watering is carried out at proper time, and fertilization and weeding are carried out in time; the water content of the matrix soil is kept at 53%, and the air humidity is controlled to be above 85%. After the scions take roots and grow leaves, 2 percent of monopotassium phosphate and 0.8 percent of urea are sprayed on the leaves.
6) Transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into holes with the depth of 9cm during transplanting, putting the seedlings into the centers of the holes during planting to enable root systems to stretch, filling surface-layer fine soil into the holes to remove impurities, filling half of the soil, properly pressing the soil by hands to enable the root systems to be in close contact with the soil, continuously filling the soil, then stepping on the soil by feet, keeping the row spacing of the plants to be 1.2m multiplied by 1.5m during transplanting, and planting 350 plants per mu.
Example 8
1) Selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing 73% peat soil and 27% vermiculite, spraying the mixed substrate with 850 times of 65% wettable carbendazim powder solution to disinfect the cutting substrate, and covering with mulching film for 2 days after the substrate is thoroughly watered for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting, shearing the cutting into 6 cm-long cutting slips, wherein when the cutting is sheared, the upper end adopts a flat edge, the lower end adopts an inclined edge, and the upper edge and the lower edge are sharp and smooth. The leaf below 3cm from the base was removed and the leaf above 3cm was retained.
3) Respectively placing 2.5cm parts of the lower ends of the cutting strips at the concentration of 150 mg.L-1Soaking in naphthylacetic acid as exogenous hormone for 1 h.
4) Cuttage: punching the sterilized matrix soil with a stick to a depth of 3cm, putting the cutting strips into the holes, and tightly pressing the matrix soil with hands to ensure that the cutting strips are tightly contacted with the matrix soil, and keeping the plant-row spacing at 6 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting, a sunshade net with 55% of light transmittance is covered on the shed top, the situation that the leaves of the cutting slips are excessively transpired, the cutting slips are dehydrated and dried up is prevented, watering is carried out at proper time, and fertilization and weeding are carried out in time; the water content of the matrix soil is kept at 53%, and the air humidity is controlled to be above 85%. After the scions take roots and grow leaves, 2 percent of monopotassium phosphate and 0.8 percent of urea are sprayed on the leaves.
6) Transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into holes with the depth of 9cm during transplanting, putting the seedlings into the centers of the holes during planting to enable root systems to stretch, filling surface-layer fine soil into the holes to remove impurities, filling half of the soil, properly pressing the soil by hands to enable the root systems to be in close contact with the soil, continuously filling the soil, then stepping on the soil by feet, keeping the row spacing of the plants to be 1.2m multiplied by 1.5m during transplanting, and planting 350 plants per mu.
Example 9
1) Selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing 73% peat soil and 27% vermiculite, spraying the mixed substrate with 850 times of 65% wettable carbendazim powder solution to disinfect the cutting substrate, and covering with mulching film for 2 days after the substrate is thoroughly watered for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting, shearing the cutting into 6 cm-long cutting slips, wherein when the cutting is sheared, the upper end adopts a flat edge, the lower end adopts an inclined edge, and the upper edge and the lower edge are sharp and smooth. The leaf below 3cm from the base was removed and the leaf above 3cm was retained.
3) Respectively placing 2.5cm parts of the lower ends of the cutting strips at the concentration of 150 mg.L-1The exogenous hormone rooting powder (containing 30% IAA and 20% NAA) is soaked for 1 h.
4) Cuttage: punching the sterilized matrix soil with a stick to a depth of 3cm, putting the cutting strips into the holes, and tightly pressing the matrix soil with hands to ensure that the cutting strips are tightly contacted with the matrix soil, and keeping the plant-row spacing at 6 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting, a sunshade net with 55% of light transmittance is covered on the shed top, the situation that the leaves of the cutting slips are excessively transpired, the cutting slips are dehydrated and dried up is prevented, watering is carried out at proper time, and fertilization and weeding are carried out in time; the water content of the matrix soil is kept at 53%, and the air humidity is controlled to be above 85%. After the scions take roots and grow leaves, 2 percent of monopotassium phosphate and 0.8 percent of urea are sprayed on the leaves.
6) Transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into holes with the depth of 9cm during transplanting, putting the seedlings into the centers of the holes during planting to enable root systems to stretch, filling surface-layer fine soil into the holes to remove impurities, filling half of the soil, properly pressing the soil by hands to enable the root systems to be in close contact with the soil, continuously filling the soil, then stepping on the soil by feet, keeping the row spacing of the plants to be 1.2m multiplied by 1.5m during transplanting, and planting 350 plants per mu.
Example 10
1) Selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing the cutting substrate by 76% peat soil and 24% vermiculite, spraying the mixed substrate with 920 times of 70% wettable carbendazim powder solution to disinfect the cutting substrate, and covering with mulching film for 3 days after the substrate is thoroughly watered for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting, shearing the cutting into a cutting with the length of 7.5cm, wherein when the cutting is sheared, the upper end adopts a flat edge, the lower end adopts an inclined edge, and the upper edge and the lower edge are sharp and smooth. The leaf below 4cm from the base was removed and the leaf above 4cm was retained.
3) Placing the lower end of the cutting strip 3.5cm away from the cutting strip at a concentration of 200 mg.L-1The exogenous hormone indolebutyric acid is soaked for 1.5 h.
4) Cuttage: punching the disinfected matrix soil by using a stick to a depth of 4cm, then placing the cutting strips into the holes and tightly pressing the matrix soil by using hands to ensure that the cutting strips are tightly contacted with the matrix soil, and keeping the plant-row spacing at 8 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting board, a sunshade net with 65% of light transmittance is covered on the shed roof, the situation that the leaves of the cutting slips are excessively transpired and are dehydrated and dried up is prevented, watering is carried out at proper time, and fertilization and weeding are carried out in time; the water content of the matrix soil is kept at 58%, and the air humidity is controlled to be above 85%. After the scions take roots and grow leaves, 2 percent of monopotassium phosphate and 0.8 percent of urea are sprayed on the leaves.
6) Transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into 11 cm-deep hole pits during transplanting, putting the seedlings into the center of the hole pits during planting to enable the root systems to stretch, filling surface-layer fine soil into the holes, removing impurities, filling half of the soil, properly pressing the soil with hands to enable the root systems to be in close contact with the soil, continuously filling the soil with the soil, stepping with feet to be solid, keeping the row spacing of the plants to be 1.2m multiplied by 1.5m during transplanting, and planting 400 plants per mu.
Example 11
1) Selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing the cutting substrate by 76% peat soil and 24% vermiculite, spraying the mixed substrate with 920 times of 70% wettable carbendazim powder solution to disinfect the cutting substrate, and covering with mulching film for 3 days after the substrate is thoroughly watered for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting, shearing the cutting into a cutting with the length of 7.5cm, wherein when the cutting is sheared, the upper end adopts a flat edge, the lower end adopts an inclined edge, and the upper edge and the lower edge are sharp and smooth. The leaf below 4cm from the base was removed and the leaf above 4cm was retained.
3) Placing the lower end of the cutting strip 3.5cm away from the cutting strip at a concentration of 200 mg.L-1The exogenous hormone naphthylacetic acid is soaked for 1.5 h.
4) Cuttage: punching the disinfected matrix soil by using a stick to a depth of 4cm, then placing the cutting strips into the holes and tightly pressing the matrix soil by using hands to ensure that the cutting strips are tightly contacted with the matrix soil, and keeping the plant-row spacing at 8 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting board, a sunshade net with 65% of light transmittance is covered on the shed roof, the situation that the leaves of the cutting slips are excessively transpired and are dehydrated and dried up is prevented, watering is carried out at proper time, and fertilization and weeding are carried out in time; the water content of the matrix soil is kept at 58%, and the air humidity is controlled to be above 85%. After the scions take roots and grow leaves, 2 percent of monopotassium phosphate and 0.8 percent of urea are sprayed on the leaves.
6) Transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into 11 cm-deep hole pits during transplanting, putting the seedlings into the center of the hole pits during planting to enable the root systems to stretch, filling surface-layer fine soil into the holes, removing impurities, filling half of the soil, properly pressing the soil with hands to enable the root systems to be in close contact with the soil, continuously filling the soil with the soil, stepping with feet to be solid, keeping the row spacing of the plants to be 1.2m multiplied by 1.5m during transplanting, and planting 400 plants per mu.
Example 12
1) Selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing the cutting substrate by 76% peat soil and 24% vermiculite, spraying the mixed substrate with 920 times of 70% wettable carbendazim powder solution to disinfect the cutting substrate, and covering with mulching film for 3 days after the substrate is thoroughly watered for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting, shearing the cutting into a cutting with the length of 7.5cm, wherein when the cutting is sheared, the upper end adopts a flat edge, the lower end adopts an inclined edge, and the upper edge and the lower edge are sharp and smooth. The leaf below 4cm from the base was removed and the leaf above 4cm was retained.
3) Placing the lower end of the cutting strip 3.5cm away from the cutting strip at a concentration of 200 mg.L-1The exogenous hormone rooting powder (containing 30% IAA and 20% NAA) is soaked for 1.5 h.
4) Cuttage: punching the disinfected matrix soil by using a stick to a depth of 4cm, then placing the cutting strips into the holes and tightly pressing the matrix soil by using hands to ensure that the cutting strips are tightly contacted with the matrix soil, and keeping the plant-row spacing at 8 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting board, a sunshade net with 65% of light transmittance is covered on the shed roof, the situation that the leaves of the cutting slips are excessively transpired and are dehydrated and dried up is prevented, watering is carried out at proper time, and fertilization and weeding are carried out in time; the water content of the matrix soil is kept at 58%, and the air humidity is controlled to be above 85%. After the scions take roots and grow leaves, 2 percent of monopotassium phosphate and 0.8 percent of urea are sprayed on the leaves.
6) Transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into 11 cm-deep hole pits during transplanting, putting the seedlings into the center of the hole pits during planting to enable the root systems to stretch, filling surface-layer fine soil into the holes, removing impurities, filling half of the soil, properly pressing the soil with hands to enable the root systems to be in close contact with the soil, continuously filling the soil with the soil, stepping with feet to be solid, keeping the row spacing of the plants to be 1.2m multiplied by 1.5m during transplanting, and planting 400 plants per mu.
Example 13
1) Selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing 78% peat soil and 22% vermiculite, spraying the mixed substrate with 950 times of 90% wettable carbendazim powder solution to disinfect the cutting substrate, and covering with mulching films for 4 days after the substrate is thoroughly watered for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch with rich in vitro nutrition and short internode spacing as a cutting, cutting the cutting into 8 cm-long cutting slips, wherein when the cutting is carried out, the upper end adopts a flat edge, the lower end adopts an inclined edge, and the upper edge and the lower edge are sharp and smooth. The leaf below 4.5cm from the base was removed and the leaf above 4.5cm was retained.
3) Placing the lower end of the cutting strip 4.5cm away from the cutting strip at a concentration of 300 mg.L-1The exogenous hormone indolebutyric acid is soaked for 1.8 h.
4) Cuttage: punching the disinfected matrix soil by using a stick to a depth of 4.5cm, then placing the cutting strips into the holes and tightly pressing the matrix soil by using hands to ensure that the cutting strips are tightly contacted with the matrix soil, and keeping the plant-row spacing at 8 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting board, a sunshade net with the light transmittance of 68 percent is covered on the shed roof, the situation that the leaves of the cutting slips are excessively transpired and are dehydrated and dried up is prevented, and watering, fertilizing and weeding are carried out timely; the water content of the matrix soil is kept at 63%, and the air humidity is controlled to be above 85%. After the scions take roots and grow leaves, 2 percent of monopotassium phosphate and 0.8 percent of urea are sprayed on the leaves.
6) Transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into holes with the depth of 11.5cm during transplanting, putting the seedlings into the centers of the holes during planting to spread root systems, filling fine soil on surface layers into the holes, removing impurities, filling half of the soil, properly pressing the soil with hands to enable the root systems to be in close contact with the soil, continuously filling the soil with the soil, stepping with feet to achieve the effect, keeping the row spacing of the plants to be 1.2m multiplied by 1.5m during transplanting, and planting 390 plants per mu.
Example 14
1) Selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing 78% peat soil and 22% vermiculite, spraying the mixed substrate with 950 times of 90% wettable carbendazim powder solution to disinfect the cutting substrate, and covering with mulching films for 4 days after the substrate is thoroughly watered for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch with rich in vitro nutrition and short internode spacing as a cutting, cutting the cutting into 8 cm-long cutting slips, wherein when the cutting is carried out, the upper end adopts a flat edge, the lower end adopts an inclined edge, and the upper edge and the lower edge are sharp and smooth. The leaf below 4.5cm from the base was removed and the leaf above 4.5cm was retained.
3) Placing the lower end of the cutting strip 4.5cm away from the cutting strip at a concentration of 300 mg.L-1The exogenous hormone naphthylacetic acid is soaked for 1.8 h.
4) Cuttage: punching the disinfected matrix soil by using a stick to a depth of 4.5cm, then placing the cutting strips into the holes and tightly pressing the matrix soil by using hands to ensure that the cutting strips are tightly contacted with the matrix soil, and keeping the plant-row spacing at 8 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting board, a sunshade net with the light transmittance of 68 percent is covered on the shed roof, the situation that the leaves of the cutting slips are excessively transpired and are dehydrated and dried up is prevented, and watering, fertilizing and weeding are carried out timely; the water content of the matrix soil is kept at 63%, and the air humidity is controlled to be above 85%. After the scions take roots and grow leaves, 2 percent of monopotassium phosphate and 0.8 percent of urea are sprayed on the leaves. .
6) Transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into holes with the depth of 11.5cm during transplanting, putting the seedlings into the centers of the holes during planting to spread root systems, filling fine soil on surface layers into the holes, removing impurities, filling half of the soil, properly pressing the soil with hands to enable the root systems to be in close contact with the soil, continuously filling the soil with the soil, stepping with feet to achieve the effect, keeping the row spacing of the plants to be 1.2m multiplied by 1.5m during transplanting, and planting 390 plants per mu.
Example 15
1) Selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing 78% peat soil and 22% vermiculite, spraying the mixed substrate with 950 times of 90% wettable carbendazim powder solution to disinfect the cutting substrate, and covering with mulching films for 4 days after the substrate is thoroughly watered for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch with rich in vitro nutrition and short internode spacing as a cutting, cutting the cutting into 8 cm-long cutting slips, wherein when the cutting is carried out, the upper end adopts a flat edge, the lower end adopts an inclined edge, and the upper edge and the lower edge are sharp and smooth. The leaf below 4.5cm from the base was removed and the leaf above 4.5cm was retained.
3) Placing the lower end of the cutting strip 4.5cm away from the cutting strip at a concentration of 300 mg.L-1The exogenous hormone rooting powder (containing 30% IAA and 20% NAA) is soaked for 1.8 h.
4) Cuttage: punching the disinfected matrix soil by using a stick to a depth of 4.5cm, then placing the cutting strips into the holes and tightly pressing the matrix soil by using hands to ensure that the cutting strips are tightly contacted with the matrix soil, and keeping the plant-row spacing at 8 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting board, a sunshade net with the light transmittance of 68 percent is covered on the shed roof, the situation that the leaves of the cutting slips are excessively transpired and are dehydrated and dried up is prevented, and watering, fertilizing and weeding are carried out timely; the water content of the matrix soil is kept at 63%, and the air humidity is controlled to be above 85%. After the scions take roots and grow leaves, 2 percent of monopotassium phosphate and 0.8 percent of urea are sprayed on the leaves.
6) Transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into holes with the depth of 11.5cm during transplanting, putting the seedlings into the centers of the holes during planting to spread root systems, filling fine soil on surface layers into the holes, removing impurities, filling half of the soil, properly pressing the soil with hands to enable the root systems to be in close contact with the soil, continuously filling the soil with the soil, stepping with feet to achieve the effect, keeping the row spacing of the plants to be 1.2m multiplied by 1.5m during transplanting, and planting 390 plants per mu.
Experimental example: to further illustrate the scientificity and rationality of the present invention, the following experimental studies of methodology were performed:
1, research on the cuttage survival rate of the nitraria tangutorum twigs processed by different exogenous hormones:
1.1 test varieties and reagent sources:
the test varieties are: nitraria flower (Sophora davidii)
Reagent: indolebutyric acid (IBA), naphthylacetic acid (NAA) and rooting powder (ABT1, 30% IAA and 20% NAA) were purchased from Sigma.
1.2 design of the experiment
1.2.1 selection of exogenous hormones: selecting 3 exogenous hormones of indolebutyric acid (IBA), naphthylacetic acid (NAA) and rooting powder (ABT1, containing 30% IAA and 20% NAA);
1.2.2 setting of the concentration of exogenous hormone: the concentrations of 3 exogenous hormones were set to 0, 100 mg.L, respectively-1、200mg·L-1、400mg·L-14 gradients.
1.2.3 blank control group: and (4) distilled water.
1.2.4 soaking time of the lower end of the cutting in exogenous hormone: soaking for 0, 1 and 2 hours respectively;
1.2.5 cutting medium: the method comprises the steps of mixing 75% of peat soil and 25% of vermiculite, spraying and disinfecting a seedbed and a substrate by using 800-1000 times of 80% wettable carbendazim powder solution on the mixed substrate, and covering with a mulching film for 3 days after the mixed substrate is thoroughly watered for later use;
1.3 results of the experiment
1.3.1 data statistics formula:
the survival rate is the number of surviving plants/the number of cuttage plants multiplied by 100 percent
The rooting rate is the number of rooted plants/the number of cuttage plants multiplied by 100 percent
Root effect index is the average root length multiplied by the number of roots/number of cuttings
1.3.2 cutting the soaked cutting slips on a substrate seedling bed, and counting the survival rate for 60 days, wherein the survival rate is shown in a table 1.
TABLE 1 Effect of different hormone treatments on the cutting of the tenderness of Nitraria sibirica pall
Figure BDA0002378088900000201
Figure BDA0002378088900000211
As a result: from the above table, it can be seen that the exogenous hormone IBA treatment is optimal, IBA concentration of 100 mg.L-1And the soaking time of the cutting is 2 hours.
1.3.3 rooting rate and root effect index of hormone treatment, the results are shown in Table 2.
TABLE 2 rooting percentage and root effect index under partial different hormone treatments
Figure BDA0002378088900000212
As a result: as can be seen from the above table, the amount of cuttings is 100 mg.L-1After being soaked in IBA solution for 2 hours, the cuttage rooting rate is better, the rooting rate reaches 75.00%, and the root system effect index is 3.78.
2 different cuttage methods for carrying out cuttage contrast experiment on Nitraria sibirica pall
2.1 test varieties: nitraria flower (Sophora davidii)
2.2 a cuttage method:
2.2.1 patent No. CN201710964389.5, cutting propagation method of Ardisia japonica
2.2.1.1 preparation of cutting slips: placing the base part of the cutting shoot in 800 times of 50% carbendazim wettable powder for soaking for 30min, taking out, draining off water in a shade until the surface is anhydrous, placing the base part in a rooting inducer for soaking for 6h, taking out, draining off water in a shade until the surface is anhydrous, and reserving for later use;
2.2.1.2 preparation of the matrix: the matrix consists of peat soil, vermiculite and fine river sand according to the volume ratio of 2:1: 1; disinfecting the matrix by using 0.3% of potassium permanganate aqueous solution in parts by mass, then thoroughly leaching with water, and thoroughly leaching with 0.3% of hydrogen peroxide in parts by mass.
2.2.1.3 cuttage method: a shading shed with the light transmittance of 45% is erected above the seedling bed, the plant spacing of the cutting slips in the seedling bed is 9cm, the cutting slips are inserted into the matrix after inclining 45 degrees relative to the vertical direction during cutting, and the insertion depth is 2/3 of the length of the cutting slips.
2.2.2 CN201410841544.0 cutting propagation method of anisetree bark
2.2.2.1 preparation of cutting slips: a) selection and treatment of cutting shoots: selecting a strong branch, cutting the branch into cutting slips, soaking the base parts of the cutting slips in 0.3% potassium permanganate solution disinfectant for 0.5h, fishing out, and airing in a shade until no water drops on the surface; and soaking in the rooting inducer for 2h, taking out, slightly airing, and draining off surface water drops.
2.2.2.2 preparation of the matrix: preparation of the substrate and the seedbed: the cuttage matrix is peat soil, perlite, vermiculite and fine river sand with the volume ratio of 1:1, and 1000 times of carbendazim liquid disinfectant is used. Sterilizing to obtain a seedbed with the thickness of 15 cm;
2.2.2.3 cutting method: cutting the cutting slips in the seedbed matrix in an inclined manner of 40-50 degrees, wherein the depth is 7-10 cm;
2.2.3 the process of the invention:
2.2.3.1 preparation of cutting slips: treating the lower end of the cutting strip for 3.5cm at a concentration of 100 mg.L-1Soaking the exogenous hormone indolebutyric acid in the solution for 2 hours;
2.2.3.2 preparation of the matrix: selecting soil with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing a cutting matrix consisting of 75% peat soil and 25% vermiculite, spraying the mixed matrix with 900 times of 80% wettable carbendazim powder solution to disinfect the seedbed and the matrix, and covering with a mulching film for 3 days after the mixed matrix is thoroughly watered for later use;
2.2.3.3 cuttage method: punching the disinfected matrix soil by using a stick to make the depth of the hole be 3.5cm, then placing the cutting strip into the hole and tightly pressing the matrix soil by using hands to make the cutting strip tightly contact with the matrix soil, and keeping the plant-row spacing at 7 cm;
2.3 cutting results
After the cutting is finished, a small arched shed covered with a plastic film is built on the method, a sunshade net with the light transmittance of 60% is covered on the shed roof, the situation that the leaves of the cutting slips are excessively transpired and the cutting slips are dehydrated and dried up is prevented, and watering, fertilizing and weeding are carried out timely; the water content of the matrix soil is kept at 60%, and the air humidity is controlled to be above 85%. After the scions take roots and grow leaves, 2 percent of monopotassium phosphate and 0.8 percent of urea are sprayed on the leaves. The results are shown in Table 3.
TABLE 3 comparative experimental results of different methods
Figure BDA0002378088900000231
As a result: the invention has high survival rate, rooting rate, average root length and root system index.
And (4) conclusion: in conclusion, the preferred method of the invention is optimized for treatment with exogenous hormone IBA at a concentration of 100 mg.L-1The soaking time of the cutting slips is 2 hours, the cutting rooting rate is better, the rooting rate reaches 75.00%, and the root system effect index is 3.78; the method is simple and easy to implement, can realize the cuttage and rapid propagation of the nitraria tangutorum bobr within a short time, has the cuttage survival rate of 75.00 percent, and provides an effective method for the nitraria tangutorum bobr in aspects of vegetation recovery, water and soil conservation ecological benefits, forage grass development and the like.
While the invention has been described in detail in the foregoing by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that certain changes and modifications may be made therein based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims (10)

1. A method for cutting propagation of a nitraria tangutorum bobr twig is characterized by comprising the following steps:
1) selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing 70-80% of peat soil and 20-30% of vermiculite, spraying 60-100% of a wettable carbendazim powder 800-1000 times of solution on the mixed substrate to sterilize the cutting substrate, and covering with a mulching film for 2-4 days after thorough watering for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting, and cutting the cutting into cutting strips with the length of 5-9 cm;
3) respectively soaking 2-5 cm parts of the lower ends of the cutting strips obtained in the step 2) in exogenous hormone for 0.5-2 h, wherein the exogenous hormone is one of indolebutyric acid, naphthylacetic acid and rooting powder, the rooting powder contains 20-40% of auxin and 10-30% of naphthylacetic acid, and each exogenous hormone containsThe concentration of the hormone is 100-400 mg.L-1；
4) Cuttage: punching the disinfected matrix soil by using a stick to a depth of 2-5 cm, then placing the cutting strips into the holes, tightly pressing the matrix soil by using hands to enable the cutting strips to be in close contact with the matrix soil, and keeping the plant-row spacing at 5-9 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting, a sunshade net with the light transmittance of 50-70% is covered on the shed top, the situation that the leaves of the cutting slips are excessively transpired and the cutting slips are dehydrated and dried up is prevented, and watering, fertilizing and weeding are carried out timely;
6) transplanting and planting: and (3) checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into holes with the depth of 8-12 cm during transplanting, putting the seedlings into the centers of the holes during transplanting to enable root systems to be spread, filling fine soil on surface layers into the holes to remove impurities, keeping the row spacing of the seedlings at 1.2m multiplied by 1.5m during transplanting, and planting 350-400 plants per mu.
2. The propagation method as claimed in claim 1, characterized in that it comprises the following steps:
1) selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing 73-78% of peat soil and 23-27% of vermiculite, spraying 70-90% of wettable carbendazim powder 850-950 times of solution on the mixed substrate to sterilize, and covering with a mulching film for 2.5-3.5 days after thorough watering for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting, and cutting the cutting into cutting strips with the length of 6-8 cm;
3) respectively soaking 3-4 cm parts of the lower ends of the cutting strips obtained in the step 2) in exogenous hormones for 0.8-1.8 h, wherein the exogenous hormones are one of indolebutyric acid, naphthylacetic acid and rooting powder, the rooting powder contains 25-35% of auxin and 15-25% of naphthylacetic acid, and the concentration of each exogenous hormone is 200-300 mg.L-1；
4) Cuttage: punching the disinfected matrix soil by using a stick to a depth of 3-4 cm, then placing the cutting strips into the holes and tightly pressing the matrix soil by using hands to ensure that the cutting strips are tightly contacted with the matrix soil, and keeping the plant-row spacing at 6-8 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting, a sunshade net with the light transmittance of 55-65% is covered on the shed top, the situation that the leaves of the cutting slips are excessively transpired and the cutting slips are dehydrated and dried up is prevented, and watering, fertilizing and weeding are carried out timely;
6) transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into holes with the depth of 9-11 cm during transplanting, putting the seedlings into the centers of the holes during transplanting to enable the root systems to be spread, filling fine soil on surface layers into the holes to remove impurities, keeping the row spacing of the seedlings at 1.2m multiplied by 1.5m during transplanting, and planting 360-390 plants per mu.
3. The propagation method as claimed in claim 2, characterized in that it comprises the following steps:
1) selection of the matrix: selecting a substrate with sufficient nutrients and good air permeability and water retention capacity as a cutting seedbed, setting the seedbed to be 2m multiplied by 4m, mixing 75% peat soil and 25% vermiculite, spraying 80% wettable carbendazim powder 900 times solution on the mixed substrate to sterilize, and covering with mulching film for 3 days after thorough watering for later use;
2) obtaining the cutting slips: selecting the middle upper part of a 1-year-old branch which is rich in nutrition in vitro and short in internode spacing as a cutting, and cutting the cutting into cutting strips with the length of 7 cm;
3) respectively soaking 3.5cm parts of the lower ends of the cutting strips obtained in the step 2) in exogenous hormones for 2 hours, wherein the exogenous hormones are one of indolebutyric acid, naphthylacetic acid and rooting powder, the rooting powder contains 30% of auxin and 20% of naphthylacetic acid, and each exogenous hormone is 100 mg.L-1；
4) Cuttage: punching the disinfected matrix soil by using a stick to make the depth of the hole be 3.5cm, then placing the cutting strip into the hole and tightly pressing the matrix soil by using hands to make the cutting strip tightly contact with the matrix soil, and keeping the plant-row spacing at 7 cm;
5) managing after the insertion: after the cutting is finished, a small arched shed covered with a plastic film is built on the cutting board, a sunshade net with the light transmittance of 60 percent is covered on the shed roof, the situation that the leaves of the cutting slips are excessively transpired and are dehydrated and dried up is prevented, and watering, weeding and fertilizing are carried out timely;
6) transplanting and planting: checking the rooting condition of the cutting seedlings after 60 days of cutting, transplanting if the cutting seedlings have rooted, beating the prepared land blocks into 10 cm-deep hole pits during transplanting, putting the seedlings into the center of the hole pits during planting to enable the root systems to be spread, filling surface-layer fine soil into the holes to remove impurities, keeping the row spacing of the seedlings at 1.2m multiplied by 1.5m during transplanting, and planting 370 plants per mu.
4. The propagation method as claimed in any one of claims 1 to 3, wherein the cutting shoot in step 2) is cut by a flat edge at the upper end and a slant edge at the lower end, wherein the upper and lower edges are sharp and smooth, and the blades with the base part less than 2-5 cm are removed and the blades with the base part more than 2-5 cm are reserved.
5. The propagation method of claim 4, wherein in the step 2), when cutting the cutting shoot, the upper end of the cutting shoot adopts a flat edge, the lower end of the cutting shoot adopts an inclined edge, the upper edge and the lower edge are sharp and smooth, the blades with the base part less than 3-4 cm are removed, and the blades with the base part more than 3-4 cm are reserved.
6. The propagation method of claim 5, wherein in the step 2), when cutting the cutting, the upper end of the cutting is a flat edge, the lower end of the cutting is a slant edge, the upper and lower edges are sharp and smooth, the blades with the base part less than 3.5cm are removed, and the blades with the base part more than 3.5cm are reserved.
7. The propagation method as claimed in any one of claims 1 to 3, wherein the timely watering after cuttage in the management after cuttage in the step 5) means that the moisture content of the matrix soil is kept between 50% and 70%, and the air humidity is controlled to be more than 85%.
8. The propagation method of claim 7, wherein timely watering after cuttage in the management after cuttage in the step 5) means that the moisture content of matrix soil is kept between 55% and 65%, and the air humidity is controlled to be above 85%.
9. The propagation method of claim 8, wherein timely watering after cutting in the management after cutting in step 5) means that the moisture content of the matrix soil is maintained at 60% and the air humidity is controlled to be above 85%.
10. The propagation method as claimed in any one of claims 1 to 3, wherein the timely weeding and fertilizing in the management after the cuttings in the step 5) are implemented by spraying 2% of monopotassium phosphate and 0.8% of urea on the leaf surfaces after the scions root and grow leaves.
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