CN114793656B - Cutting propagation method of radix tinosporae seedlings - Google Patents
Cutting propagation method of radix tinosporae seedlings Download PDFInfo
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- CN114793656B CN114793656B CN202210678828.7A CN202210678828A CN114793656B CN 114793656 B CN114793656 B CN 114793656B CN 202210678828 A CN202210678828 A CN 202210678828A CN 114793656 B CN114793656 B CN 114793656B
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- 238000005520 cutting process Methods 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 34
- 238000009395 breeding Methods 0.000 claims abstract description 25
- 230000001488 breeding effect Effects 0.000 claims abstract description 25
- 238000002791 soaking Methods 0.000 claims abstract description 14
- 238000002360 preparation method Methods 0.000 claims abstract description 12
- 238000009966 trimming Methods 0.000 claims abstract description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 56
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims description 28
- 241000133430 Tinospora Species 0.000 claims description 21
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- 239000010451 perlite Substances 0.000 claims description 20
- 235000019362 perlite Nutrition 0.000 claims description 20
- 239000010455 vermiculite Substances 0.000 claims description 20
- 235000019354 vermiculite Nutrition 0.000 claims description 20
- 229910052902 vermiculite Inorganic materials 0.000 claims description 20
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 claims description 12
- 239000003415 peat Substances 0.000 claims description 4
- 239000002689 soil Substances 0.000 claims description 4
- 239000000758 substrate Substances 0.000 claims description 2
- 241000345998 Calamus manan Species 0.000 abstract description 25
- 235000012950 rattan cane Nutrition 0.000 abstract description 25
- 239000000463 material Substances 0.000 abstract description 18
- 241001643425 Tinospora sinensis Species 0.000 abstract description 12
- 239000011159 matrix material Substances 0.000 description 18
- 239000000203 mixture Substances 0.000 description 18
- 230000000877 morphologic effect Effects 0.000 description 18
- 238000010008 shearing Methods 0.000 description 18
- 238000005507 spraying Methods 0.000 description 18
- 230000012010 growth Effects 0.000 description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 17
- 239000008213 purified water Substances 0.000 description 16
- 238000002474 experimental method Methods 0.000 description 11
- 238000004140 cleaning Methods 0.000 description 9
- 230000000052 comparative effect Effects 0.000 description 9
- 239000007788 liquid Substances 0.000 description 9
- 238000002156 mixing Methods 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 239000008946 yang xin Substances 0.000 description 9
- 239000012452 mother liquor Substances 0.000 description 8
- 239000010413 mother solution Substances 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 6
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 240000003428 Tinospora crispa Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000021022 fresh fruits Nutrition 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 241000218164 Menispermaceae Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
Abstract
The invention belongs to the technical field of seedling breeding, and particularly relates to a cutting propagation method of radix tinosporae seedlings. A cutting propagation method of radix tinosporae seedlings comprises the following steps: preparation of explants: selecting annual non-lignified tinospora sinensis rattan, and trimming leaves for later use; cutting: soaking the explant in rooting agent, cutting in a cutting bed to make the stem contact with the cutting bed; and (3) breeding management: and the cutting is kept in a dry and wet state, so that direct sunlight is prevented. The invention creatively selects annual non-lignified rattan to trim and prepare the explant, and successfully breeds the radix tinosporae seedling. Compared with lignified or semi-lignified rattan, the method has the advantages of being capable of taking materials for a long time, taking materials all year round, high in breeding coefficient and the like.
Description
Technical Field
The invention belongs to the technical field of seedling breeding, and particularly relates to a cutting propagation method of radix tinosporae seedlings.
Background
The radix tinosporae, chinese medicine name, is the dry root tuber of radix tinosporae or radix tinosporae of Menispermaceae, is picked in autumn and winter, removed fibrous root, cleaned, sun dried, and distributed in China, east China, south China and southwest. The sources of the medicinal materials at the present stage are mainly wild, the wild medicinal materials are excavated and damaged seriously, the supply quantity is reduced year by year, and the resource problem is outstanding.
The artificial cultivation has the advantages of low seedling breeding quantity, long period and single mode, so that the seedling unit price is high, the planting enthusiasm is low, and the industrial development is seriously restricted. Therefore, in order to meet the market demand, an effective rapid propagation method of the radix tinosporae seedlings needs to be established, so that the high-quality radix tinosporae seedlings can be stably produced, market gaps can be made up, and the industrial development can be promoted.
At present, the seedling breeding of the tinospora cordifolia is mainly seed breeding and root tuber breeding, and the tissue culture breeding and cutting breeding are fresh. The seedlings obtained by seed propagation have strong disease resistance and vigorous growth vigor, but the seeds of the tinospora sinensis required by propagation are difficult to obtain, and the male-female ratio of the field tinospora sinensis plants is 1:50, the female plant can bloom more than 4 years old, so that the female plant has less flowers and low fruiting quantity, and 1kg of fresh fruits can be cleaned to obtain about 100g of seeds, the seed yield is low, the price of 1kg of fresh fruits in recent years is 1000-1200 yuan, and the seeds are not sold. The root seedlings obtained by root tuber propagation are irregular in emergence and high in production cost, the used root tuber is the radix tinosporae medicinal material after being processed in the production place, and the dry rate of the medicinal material is about 2:1, the market price of the radix tinosporae medicinal material is 250-300 yuan/kg in recent years, the price of the root tuber is 125-150 yuan/kg, the root tuber required by 1 plant root seedling is about 20g, the planting density is 8000 plants/mu, the root tuber required by mu planting is about 160kg, and the cost is 20000-24000 yuan. The tissue culture seedlings obtained by tissue culture propagation have weak disease resistance, poor growth potential and long propagation period, and the propagation period is calculated from the beginning of material drawing to the time of seedling formation and needs 9-11 months. The cuttage seedling obtained by cuttage propagation can inherit the excellent characters of a parent plant, but the cuttage propagation method of the tinospora cordifolia disclosed in the prior patent literature adopts lignified or semi-lignified rattan or stem segments as materials, and the standard can be met only by more than 1 year under the condition of normal growth of plants, so that the propagation period is long, the propagation coefficient is low, and in addition, sand beds with specific structures are required to be manufactured, so that popularization and use are not facilitated.
Disclosure of Invention
Therefore, the invention aims to overcome the defects that the cutting propagation method of the radix tinosporae needs to take lignified or semi-lignified rattan as a material, the propagation period is long, the materials are limited, the propagation coefficient is low, a sand bed with a specific structure is needed, popularization and application are not facilitated, and the like in the prior art, and further provides the cutting propagation method of the radix tinosporae seedling.
Therefore, the invention provides the following technical scheme:
the invention provides a cuttage propagation method of radix tinosporae seedlings, which comprises the following steps:
preparation of explants: selecting annual non-lignified tinospora root stems, and trimming leaves for later use;
cutting: soaking the explant in rooting agent, cutting in a cutting bed to make the stem contact with the cutting bed;
and (3) breeding management: and the cutting is kept in a dry and wet state, so that direct sunlight is prevented.
Optionally, the rooting agent comprises naphthalene acetic acid with the concentration of 125-300mg/L and indolebutyric acid with the concentration of 125-300 mg/L;
preferably, the rooting agent comprises naphthalene acetic acid with the concentration of 200-250mg/L and indolebutyric acid with the concentration of 200-250 mg/L;
and/or the rooting agent also comprises ethanol with the concentration of 1.0ml/L-1.2 ml/L.
Optionally, the explant is soaked in the rooting agent for 15-20min, and the rooting agent is 2/3 of the stem section of the explant.
Optionally, the length of the stem section of the explant is left to be 1.5-2.0 cm.
Optionally, the stem segment of the explant comprises at least one axillary bud;
and/or, cutting each leaf on the stem segment of the explant by 1/3-1/2.
Optionally, the cutting depth is 1.0-1.5cm.
Optionally, the slotting machine comprises a volume ratio of (8-10): (1-1.2): (1-1.2), perlite and vermiculite.
Optionally, the substrate is peat soil.
The peat soil is typically, but not limited to, pink peat soil, the organic matter content is up to 98%, the trace elements are contained in a small amount, the fiber thickness is 10-30 mm, the pH is 5.5-6.5, and the humidity is 35% -55%.
Optionally, the slotting machine is a plug slotting machine.
Optionally, the cutting time is annual.
The technical scheme of the invention has the following advantages:
the method for breeding the radix tinosporae seedlings in the prior art generally refers to the traditional technical experience in the fields of fruit trees and the like, and selects semi-lignified or lignified vines to prepare the explants, so that the method has the defects of limited material taking time, low breeding coefficient, long breeding period and the like. Compared with semi-lignified stems and semi-lignified rattan, the material has the advantages of early material taking time, short breeding period, unlimited material taking time, availability of materials throughout the year, high propagation coefficient and the like. The specific expression is as follows: (1) the cutting nursery built in 6 months can meet the requirement of drawing materials under the condition of 9 months rattan growth in the current year, and the semi-lignified stem segments and the semi-lignified rattan can be drawn materials in 6 months in the next year; (2) under the premise of keeping the sampling time consistent, the number of the non-lignified stem segments can be obtained from 60cm rattan, the number of the semi-lignified stem segments is about 28-32, the number of the semi-lignified stem segments is about 7-8, and the number of the semi-lignified rattan is about 3-4; (3) after the cutting nursery is built for 3 months, the non-lignified stem segments can be obtained from the whole year, and the semi-lignified stem segments or semi-lignified rattan need to be intermittently picked due to the limitation of rattan age. According to the cuttage propagation method for the tinospora root seedlings, provided by the invention, the rooting agent is limited, the non-lignified explant stem of the tinospora root can be induced to root, the rooting rate after 60 days reaches 88.89%, the average root number reaches 9.29, the average root length can reach 3.40cm, and the rooting condition is good.
In addition, the radix tinosporae cutting propagation method disclosed in the prior patent document does not track and record the seedling growth vigor after rooting and transplanting of cuttings in detail, is not beneficial to comprehensive assessment of seedling quality, and may have the following problems: rattan can cause seedling lodging due to overlong overground parts and long sprouting points of new buds away from the ground; the semi-lignified stem segments or rattan have long growth years, endogenous toxins accumulate, and diseases are easy to occur in later growth; the semi-lignified stem segments or rattan are grown for more than 3 years, the nutrition growth stage is skipped after the cuttings root and become seedlings, the seedlings are directly in the reproduction growth stage, the seedling resistance is low, and the survival rate after transplanting is low. According to the cuttage propagation method of the radix tinosporae seedlings, provided by the invention, the radix tinosporae seedlings bred by the method have the average height of 17.14cm, the average stem thickness of 1.42mm at the 3cm position, 2-3 mature leaves, 4-6 young leaves and 5-8 bud points, no obvious diseases or insect pests occur, and good growth vigor under the proper growth environment condition. In the prior art, tracking investigation is not carried out on the overground part growth condition of the root-rooted tinospora root cutting, the prediction of the field growth trend and the comprehensive evaluation of the seedling quality after the tinospora seedling is transplanted are not facilitated, the seedling quality is related to overground growth condition and underground rooting condition indexes, the rooting rate only partially illustrates the underground rooting condition, the overground growth condition comprises plant height, crown width, stem thickness, leaf number and the like, the underground rooting condition comprises rooting rate, root length, root thickness, root number and the like, the seedling quality can be accurately judged by tracking and recording the indexes accurately, and the follow-up plant growth prediction expectation is improved.
According to the cuttage propagation method for the radix tinosporae seedlings, the slotting machine can be a plug slotting machine, a slotting machine with a specific structure is not required to be customized, and popularization and use are facilitated.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a comparative graph of root growth for examples of the present invention and comparative examples;
fig. 2 is a 6-month growth chart after transplanting of the seedling provided in example 1 of the present invention.
Detailed Description
The following examples are provided for a better understanding of the present invention and are not limited to the preferred embodiments described herein, but are not intended to limit the scope of the invention, any product which is the same or similar to the present invention, whether in light of the present teachings or in combination with other prior art features, falls within the scope of the present invention.
The specific experimental procedures or conditions are not noted in the examples and may be followed by the operations or conditions of conventional experimental procedures described in the literature in this field. The reagents or apparatus used were conventional reagent products commercially available without the manufacturer's knowledge.
Example 1
The embodiment provides a fast cutting propagation method of radix tinosporae seedlings, 9 months in 2021, relevant experiments are carried out in the original village of white Sha Zhenping of Yangxin county of Huang Danshi in Hubei province, and the specific steps comprise:
preparation of explants: selecting annual non-lignified tinospora sinensis rattan, shearing off stem segments connected with axillary buds and morphological upper ends by using a branch shear, shearing off half of leaves, reserving the length of the stem segments at the morphological lower ends to be 1.5-2.0cm, and chamfering a notch for later use;
preparing a rooting agent mother solution: respectively taking 25g of naphthalene acetic acid and 25g of indolebutyric acid, dissolving with 100ml of ethanol, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the product;
preparing a rooting agent: respectively taking 10ml of rooting agent mother liquor, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the rooting agent;
and (3) slotting machine assembly: matrix (brand name is denmark) according to volume proportion: perlite: vermiculite = 8:1:1, respectively taking corresponding amounts of matrix, perlite and vermiculite, fully and uniformly mixing, then filling the mixture into a 72-hole PE plug, and uniformly spraying until the mixture is thoroughly wet for later use;
explant treatment: soaking the explant in prepared rooting agent (250 mg/L NAA+250mg/L IBA) for 15min, wherein the liquid level is required to be 2/3 of the stem section of the explant;
and (3) breeding management: immediately and uniformly spraying the plug with a sprayer until the plug is wetted, cleaning dirt on leaf surfaces, keeping moisture during management, maintaining a dry and wet state, building a sunshade net, and preventing direct sunlight;
after 60 days of cuttage, the tinospora root cup seedlings are washed, five seedlings are randomly taken for photographing and observing rooting conditions, and referring to fig. 1, the rooting rate, average root number and average root length of the 10 seedlings are counted.
Example 2
The embodiment provides a fast cutting propagation method of radix tinosporae seedlings, 9 months in 2021, relevant experiments are carried out in the original village of white Sha Zhenping of Yangxin county of Huang Danshi in Hubei province, and the specific steps comprise:
preparation of explants: selecting annual non-lignified tinospora sinensis rattan, shearing off stem segments connected with axillary buds and morphological upper ends by using a branch shear, shearing off half of leaves, reserving the length of the stem segments at the morphological lower ends to be 1.5-2.0cm, and chamfering a notch for later use;
preparing a rooting agent mother solution: respectively taking 30g of naphthalene acetic acid and 30g of indolebutyric acid, dissolving with 100ml of ethanol, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the product;
preparing a rooting agent: respectively taking 10ml of rooting agent mother liquor, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the rooting agent;
and (3) slotting machine assembly: matrix (brand name is denmark) according to volume proportion: perlite: vermiculite = 8:1:1, respectively taking corresponding amounts of matrix, perlite and vermiculite, fully and uniformly mixing, then filling the mixture into a 72-hole PE plug, and uniformly spraying until the mixture is thoroughly wet for later use;
explant treatment: soaking the explant in prepared rooting agent (300 mg/L NAA+300mg/L IBA) for 15min, wherein the liquid level is required to be 2/3 of the stem section of the explant;
and (3) breeding management: immediately and uniformly spraying the plug with a sprayer until the plug is wetted, cleaning dirt on leaf surfaces, keeping moisture during management, maintaining a dry and wet state, building a sunshade net, and preventing direct sunlight;
after 60 days of cuttage, the tinospora root cup seedlings are washed clean, and the rooting rate, average root number and average root length of 10 seedlings are counted randomly.
Example 3
The embodiment provides a fast cutting propagation method of radix tinosporae seedlings, 9 months in 2021, relevant experiments are carried out in the original village of white Sha Zhenping of Yangxin county of Huang Danshi in Hubei province, and the specific steps comprise:
preparation of explants: selecting annual non-lignified tinospora sinensis rattan, shearing off stem segments connected with axillary buds and morphological upper ends by using a branch shear, shearing off half of leaves, reserving the length of the stem segments at the morphological lower ends to be 1.5-2.0cm, and chamfering a notch for later use;
preparing a rooting agent mother solution: respectively taking 20g of naphthalene acetic acid and 20g of indolebutyric acid, dissolving with 100ml of ethanol, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the product;
preparing a rooting agent: respectively taking 10ml of rooting agent mother liquor, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the rooting agent;
and (3) slotting machine assembly: matrix (brand name is denmark) according to volume proportion: perlite: vermiculite = 8:1:1, respectively taking corresponding amounts of matrix, perlite and vermiculite, fully and uniformly mixing, then filling the mixture into a 72-hole PE plug, and uniformly spraying until the mixture is thoroughly wet for later use;
explant treatment: soaking the explant in prepared rooting agent (200 mg/L NAA+200mg/L IBA) for 15min, wherein the liquid level is required to be 2/3 of the stem section of the explant;
and (3) breeding management: immediately and uniformly spraying the plug with a sprayer until the plug is wetted, cleaning dirt on leaf surfaces, keeping moisture during management, maintaining a dry and wet state, building a sunshade net, and preventing direct sunlight;
after 60 days of cuttage, the tinospora root cup seedlings are washed clean, and the rooting rate, average root number and average root length of 10 seedlings are counted randomly.
Example 4
The embodiment provides a fast cutting propagation method of radix tinosporae seedlings, 9 months in 2021, relevant experiments are carried out in the original village of white Sha Zhenping of Yangxin county of Huang Danshi in Hubei province, and the specific steps comprise:
preparation of explants: selecting annual non-lignified tinospora sinensis rattan, shearing off stem segments connected with axillary buds and morphological upper ends by using a branch shear, shearing off half of leaves, reserving the length of the stem segments at the morphological lower ends to be 1.5-2.0cm, and chamfering a notch for later use;
preparing a rooting agent mother solution: respectively taking 24g of naphthylacetic acid and 26g of indolebutyric acid, dissolving with 100ml of ethanol, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the product;
preparing a rooting agent: respectively taking 10ml of rooting agent mother liquor, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the rooting agent;
and (3) slotting machine assembly: matrix (brand name is denmark) according to volume proportion: perlite: vermiculite = 8:1:1, respectively taking corresponding amounts of matrix, perlite and vermiculite, fully and uniformly mixing, then filling the mixture into a 72-hole PE plug, and uniformly spraying until the mixture is thoroughly wet for later use;
explant treatment: soaking the explant in prepared rooting agent (240 mg/L NAA+260mg/L IBA) for 15min, wherein the liquid level is required to be 2/3 of the stem section of the explant;
and (3) breeding management: immediately and uniformly spraying the plug with a sprayer until the plug is wetted, cleaning dirt on leaf surfaces, keeping moisture during management, maintaining a dry and wet state, building a sunshade net, and preventing direct sunlight;
after 60 days of cuttage, the tinospora root cup seedlings are washed clean, and the rooting rate, average root number and average root length of 10 seedlings are counted randomly.
Example 5
The embodiment provides a fast cutting propagation method of radix tinosporae seedlings, 9 months in 2021, relevant experiments are carried out in the original village of white Sha Zhenping of Yangxin county of Huang Danshi in Hubei province, and the specific steps comprise:
preparation of explants: selecting annual non-lignified tinospora sinensis rattan, shearing off stem segments connected with axillary buds and morphological upper ends by using a branch shear, shearing off half of leaves, reserving the length of the stem segments at the morphological lower ends to be 1.5-2.0cm, and chamfering a notch for later use;
preparing a rooting agent mother solution: respectively taking 27g of naphthylacetic acid and 25g of indolebutyric acid, dissolving with 100ml of ethanol, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the product;
preparing a rooting agent: respectively taking 10ml of rooting agent mother liquor, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the rooting agent;
and (3) slotting machine assembly: matrix (brand name is denmark) according to volume proportion: perlite: vermiculite = 8:1:1, respectively taking corresponding amounts of matrix, perlite and vermiculite, fully and uniformly mixing, then filling the mixture into a 72-hole PE plug, and uniformly spraying until the mixture is thoroughly wet for later use;
explant treatment: soaking the explant in rooting agent (270 mg/L NAA+250mg/L IBA) for 15min, wherein the liquid level is 2/3 of the stem section of the explant;
and (3) breeding management: immediately and uniformly spraying the plug with a sprayer until the plug is wetted, cleaning dirt on leaf surfaces, keeping moisture during management, maintaining a dry and wet state, building a sunshade net, and preventing direct sunlight;
after 60 days of cuttage, the tinospora root cup seedlings are washed clean, and the rooting rate, average root number and average root length of 10 seedlings are counted randomly.
Example 6
The embodiment provides a fast cutting propagation method of radix tinosporae seedlings, 9 months in 2021, relevant experiments are carried out in the original village of white Sha Zhenping of Yangxin county of Huang Danshi in Hubei province, and the specific steps comprise:
preparation of explants: selecting annual non-lignified tinospora sinensis rattan, shearing off stem segments connected with axillary buds and morphological upper ends by using a branch shear, shearing off half of leaves, reserving the length of the stem segments at the morphological lower ends to be 1.5-2.0cm, and chamfering a notch for later use;
preparing a rooting agent mother solution: respectively taking 12.5g of naphthalene acetic acid and 12.5g of indolebutyric acid, dissolving with 100ml of ethanol, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the product;
preparing a rooting agent: respectively taking 10ml of rooting agent mother liquor, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the rooting agent;
and (3) slotting machine assembly: matrix (brand name is denmark) according to volume proportion: perlite: vermiculite = 8:1:1, respectively taking corresponding amounts of matrix, perlite and vermiculite, fully and uniformly mixing, then filling the mixture into a 72-hole PE plug, and uniformly spraying until the mixture is thoroughly wet for later use;
explant treatment: soaking the explant in prepared rooting agent (125 mg/L NAA+125mg/L IBA) for 15min, wherein the liquid level is required to be 2/3 of the stem section of the explant;
and (3) breeding management: immediately and uniformly spraying the plug with a sprayer until the plug is wetted, cleaning dirt on leaf surfaces, keeping moisture during management, maintaining a dry and wet state, building a sunshade net, and preventing direct sunlight;
after 60 days of cuttage, the tinospora root cup seedlings are washed, five seedlings are randomly taken for photographing and observing rooting conditions, and referring to fig. 1, the rooting rate, average root number and average root length of the 10 seedlings are counted.
Example 7
The embodiment provides a fast cutting propagation method of radix tinosporae seedlings, 9 months in 2021, relevant experiments are carried out in the original village of white Sha Zhenping of Yangxin county of Huang Danshi in Hubei province, and the specific steps comprise:
preparation of explants: selecting annual non-lignified tinospora sinensis rattan, shearing off stem segments connected with axillary buds and morphological upper ends by using a branch shear, shearing off half of leaves, reserving the length of the stem segments at the morphological lower ends to be 1.5-2.0cm, and chamfering a notch for later use;
preparing a rooting agent mother solution: respectively taking 25g of naphthalene acetic acid and 10g of indolebutyric acid, dissolving with 100ml of ethanol, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the product;
preparing a rooting agent: respectively taking 10ml of rooting agent mother liquor, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the rooting agent;
and (3) slotting machine assembly: matrix (brand name is denmark) according to volume proportion: perlite: vermiculite = 8:1:1, respectively taking corresponding amounts of matrix, perlite and vermiculite, fully and uniformly mixing, then filling the mixture into a 72-hole PE plug, and uniformly spraying until the mixture is thoroughly wet for later use;
explant treatment: soaking the explant in prepared rooting agent (250 mg/L NAA+100mg/L IBA) for 15min, wherein the liquid level is required to be 2/3 of the stem section of the explant;
and (3) breeding management: immediately and uniformly spraying the plug with a sprayer until the plug is wetted, cleaning dirt on leaf surfaces, keeping moisture during management, maintaining a dry and wet state, building a sunshade net, and preventing direct sunlight;
after 60 days of cuttage, the tinospora root cup seedlings are washed clean, and the rooting rate, average root number and average root length of 10 seedlings are counted randomly.
Comparative example 1
The comparative example provides a rapid propagation method of radix tinosporae seedlings, 9 months in 2021, and related experiments are carried out in the original village of white Sha Zhenping of Yangxin county of Huang Danshi in Hubei province, and the specific steps comprise:
preparation of explants: selecting annual non-lignified tinospora sinensis rattan, shearing off stem segments connected with axillary buds and morphological upper ends by using a branch shear, shearing off half of leaves, reserving the length of the stem segments at the morphological lower ends to be 1.5-2.0cm, and chamfering a notch for later use;
and (3) slotting machine assembly: the volume ratio of the matrix is as follows: perlite: vermiculite = 8:1:1, respectively taking a corresponding amount of matrix (brand name is Danish), perlite and vermiculite, fully and uniformly mixing, then filling the mixture into a 72-hole PE plug, and uniformly spraying until the mixture is thoroughly wet for later use;
explant treatment: soaking the explant in clear water for 15min, wherein the liquid level is required to be 2/3 of the stem section of the explant;
and (3) breeding management: immediately and uniformly spraying the plug with a sprayer until the plug is wetted, cleaning dirt on leaf surfaces, keeping moisture during management, maintaining a dry and wet state, building a sunshade net, and preventing direct sunlight;
after 60 days of cuttage, the tinospora root cup seedlings are washed, five seedlings are randomly taken for photographing and observing rooting conditions, and referring to fig. 1, the rooting rate, average root number and average root length of the 10 seedlings are counted.
Comparative example 2
The comparative example provides a fast cutting propagation method of radix tinosporae seedlings, 9 months in 2021, relevant experiments are carried out in the original village of white Sha Zhenping of Yangxin county of Huang Danshi in Hubei province, and the specific steps comprise:
preparation of explants: selecting annual non-lignified tinospora sinensis rattan, shearing off stem segments connected with axillary buds and morphological upper ends by using a branch shear, shearing off half of leaves, reserving the length of the stem segments at the morphological lower ends to be 1.5-2.0cm, and chamfering a notch for later use;
preparing a rooting agent mother solution: dissolving 2.5g of naphthalene acetic acid and 2.5g of indolebutyric acid with 100ml of ethanol, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the final product;
preparing a rooting agent: respectively taking 10ml of rooting agent mother liquor, placing into a 1L volumetric flask, fixing the volume to scale marks with purified water, and shaking uniformly to obtain the rooting agent;
and (3) slotting machine assembly: matrix (brand name is denmark) according to volume proportion: perlite: vermiculite = 8:1:1, respectively taking corresponding amounts of matrix, perlite and vermiculite, fully and uniformly mixing, then filling the mixture into a 72-hole PE plug, and uniformly spraying until the mixture is thoroughly wet for later use;
explant treatment: soaking the explant in rooting agent (25 mg/L NAA+25 mg/LIBA) for 15min to make the liquid level exceed 2/3 of the stem section of the explant;
and (3) breeding management: immediately and uniformly spraying the plug with a sprayer until the plug is wetted, cleaning dirt on leaf surfaces, keeping moisture during management, maintaining a dry and wet state, building a sunshade net, and preventing direct sunlight;
after 60 days of cuttage, the tinospora root cup seedlings are washed clean, and the rooting rate, average root number and average root length of 10 seedlings are counted randomly.
Table 1 comparison of rooting indicators for examples and comparative examples
| Name of the name | Rooting agent concentration | Rooting rate/% | Average root number/bar | Average root length/cm |
| Comparative example 1 | 0 | 53.85 | 1.71 | 2.67 |
| ComparisonExample 2 | 25mg/L NAA+25mg/L IBA | 33.33 | 2.89 | 0.95 |
| Example 1 | 250mg/L NAA+250mg/L IBA | 88.89 | 9.29 | 3.40 |
| Example 2 | 300mg/L NAA+300mg/L IBA | 82.05 | 8.06 | 3.25 |
| Example 3 | 200mg/L NAA+200mg/L IBA | 75.22 | 7.15 | 3.10 |
| Example 4 | 240mg/L NAA+260mg/L IBA | 83.46 | 9.17 | 3.32 |
| Example 5 | 270mg/L NAA+250mg/L IBA | 86.52 | 8.35 | 2.98 |
| Example 6 | 125mg/L NAA+125mg/L IBA | 63.64 | 4.00 | 2.87 |
| Example 7 | 250mg/L NAA+100mg/L IBA | 58.95 | 9.05 | 3.20 |
As shown in the table, the rooting rate, average root number and average root length of the explant can be effectively improved by soaking the explant in a rooting agent with a certain hormone concentration, the optimal rooting agent concentration at the appearance stage is 250mg/L NAA+250mg/L IBA through a gradient concentration screening test, the rooting rate of the treated explant after 60 days under the hormone concentration is 88.89%, the average root number is 9.29, the average root length is 3.40cm, the seedling provided in the example 1 is randomly taken for transplanting, the trace investigation after 6 months of transplanting growth shows that the average height of the newly-grown rattan is 17.14cm, the average stem thickness at the 3cm is 1.42cm, 2-3 young leaves are provided, 4-6 young leaves are provided, the bud points are 5-8, no obvious diseases or insect pests exist, and the growth vigor map after 6 months of transplanting is shown in fig. 2. In addition, it is known from the comparison results of comparative examples 1 and 2 that rooting is inhibited if the concentration of the rooting agent is not properly selected.
It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. And obvious variations or modifications thereof are contemplated as falling within the scope of the present invention.
Claims (7)
1. The cutting propagation method of the radix tinosporae seedlings is characterized by comprising the following steps of:
preparation of explants: selecting annual non-lignified tinospora root stems, and trimming leaves for later use;
cutting: soaking the explant in rooting agent, cutting in a cutting bed to make the stem contact with the cutting bed;
and (3) breeding management: after cutting, the cutting is maintained in a dry and wet state, so that direct sunlight is prevented;
wherein the rooting agent comprises naphthalene acetic acid with the concentration of 200-300mg/L, indolebutyric acid with the concentration of 200-300mg/L and ethanol with the concentration of 1.0ml/L-1.2 ml/L;
the slotting machine is a plug slotting machine;
the slotting machine comprises the following components in percentage by volume: 1:1, perlite and vermiculite;
the length of the stem section of the explant is 1.5-2.0cm;
the cutting depth is 1.0-1.5cm.
2. The cutting propagation method of tinospora root seedlings according to claim 1, wherein the rooting agent comprises naphthalene acetic acid with the concentration of 200-250mg/L and indolebutyric acid with the concentration of 200-250 mg/L.
3. The cutting propagation method of tinospora root seedlings according to claim 1, wherein the soaking time of the explant in the rooting agent is 15-20min, and the rooting agent is 2/3 or more than the stem sections of the explant.
4. A method of cutting propagation of a tinospora seedling according to claim 1, characterized in that the stem section of the explant comprises at least one axillary bud.
5. A cutting propagation method of tinospora seedling according to claim 1, characterized in that each leaf on the stem section of the explant is cut off by 1/3-1/2.
6. The cutting propagation method of tinospora root seedlings according to claim 1, wherein the substrate is peat soil.
7. The cutting propagation method of tinospora root seedlings according to claim 1, wherein the cutting time is annual.
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