CN106386184A - Tea-oil tree cutting and grafting integral reproduction method - Google Patents

Tea-oil tree cutting and grafting integral reproduction method Download PDF

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Publication number
CN106386184A
CN106386184A CN201610743664.6A CN201610743664A CN106386184A CN 106386184 A CN106386184 A CN 106386184A CN 201610743664 A CN201610743664 A CN 201610743664A CN 106386184 A CN106386184 A CN 106386184A
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China
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grafting
cuttage
seedbed
parts
tea
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Inventor
唐新香
吕伟军
吕剑锋
李军
谢金贵
李广寿
李胜
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Guangxi Royal Hall Biotechnology Co Ltd
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Guangxi Royal Hall Biotechnology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B17/00Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/10Process efficiency
    • Y02P20/133Renewable energy sources, e.g. sunlight

Abstract

The invention relates to the technical field of tea-oil tree nursery stock reproduction, and in particular to a tea-oil tree cutting and grafting integral reproduction method. According to the tea-oil tree cutting and grafting integral reproduction method, twigs of a wild tea-oil tree variety or a non-high-quality tea-oil tree variety are adopted to be subjected to cuttage, after cut twigs have calluses, the cut twigs are timely pulled out from a seedling growing medium, the cut twigs as root stock and high-quality tea-oil tree variety twigs are subjected to grafting, the twigs are subjected to cuttage back into the seedling growing medium in a nutrition bowl, and daily management is performed. By adopting the cutting and grafting integral reproduction method, seedlings can be produced and grown in a large scale without season constraint, the requirement on high-quality tea-oil tree variety twigs can be greatly reduced, and thus the problem that high-quality tea-oil tree variety resources are not sufficient can be solved. In addition, the seedling growing period is short, the grafting survival rate is as high as 85% and greater, seedlings can be produced and grown uninterruptedly in a whole year, seedling growing fields do not need to be changed, the seedlings can be grown in the nutrition bowl till the seedlings which survive after being grafted are delivered from nursery gardens, the cutting and grafting integral reproduction method is simple to operate, easy to learn and low in cost, and tea-oil tree seedlings which survive after being grafted are well developed in root systems, good in growing situations, fleshy and thick in leaf surfaces and free of diseases or insect pests.

Description

A kind of oil tea cuttage propagation method integrated with grafting
【Technical field】
The invention belongs to technical field of plant propagation is and in particular to a kind of oil tea cuttage propagation method integrated with grafting.
【Background technology】
Oil tea is the distinctive important edible oil materials seeds of China, strong adaptability, drought-resistant, barren, suitable southern hillses mountain Area plants.Oleum Camelliae unsaturated fatty acid content reaches 94%, is the hat in crude vegetal, is the oily and pure sky of high-quality functional health Right non-polluted edible oil.
Oil tea is with seed, cuttage or propagation by grafiting.Carry out seedling breeding and seedling with seed, need substantial amounts of improved seeds oil Tea seed, the seedling cycle longer it is impossible to meet the demand in market, and the nursery stock that breeds out is it cannot be guaranteed that keep that parent's is excellent Optimum shape, character is likely to inconsistent;Camellia oleifera Cutting Seedling Raising is the totipotency using plant cell, will shear off on excellent elite stand Branch be trained complete new plant under certain conditions.Subtropic of China area has superior climate resources condition, The season of suitable oil tea cuttage is longer, and cutting propagation easily forms scale, has that breeding coefficient is high, character is stable, simple to operate, Seedling cost is relatively low, be less prone to the advantages such as seasonal labour's anxiety, is the important technical of subtropical zone oil tea breeding. However, cutting propagation needs substantial amounts of high-quality camellia oleifera cultivar branch, and it is semi-lignified, just lignified branch, this just restricts The scale of cutting propagation and time.Branch using wild camellia oleifera cultivar or non-prime camellia oleifera cultivar carries out cuttage, cuttage Aboundresources, after cutting survival, then carries out grafting with the branch of high-quality camellia oleifera cultivar, so can large-scale production nursery, no It is subject to seasonal restrictions, greatly reduces the demand to high-quality camellia oleifera cultivar branch again, solve high-quality oil tea variety source deficiency Problem.
But the propagation method of grafting after above-mentioned first cuttage, growing-seedling period is longer, after cuttage branch cutting survival, need to continue nursery 2-3 month, treat that cutting seedling root system is mature and stable, sprouting branch that is extracting out starts lignifying, just can carry out grafting;Otherwise, during grafting With cutting seedling as stock, when cutting graft union, severe one may be extracted cutting seedling from seedbed, the lighter also can cause cutting seedling root system Impaired, even result in all of and be all torn;Even if these situations all do not occur, also need 1.5-2 month after grafting, grafting Fringe is only possible to survive, and to emerging, needs longer seedling raise period.Therefore, one piece of nursery lot, the oil that 2 batches can only be produced in 1 year Tea shoot, far can not meet the demand to fine quality oil tea Seedling for the market.
【Content of the invention】
The present invention in view of the shortcomings of the prior art, provides a kind of oil tea cuttage propagation method integrated with grafting, should Method growing-seedling period is short, and cuttage, high graft survival rate, oil tea Seedling root system easy to learn, low cost, grafting survival simple to operate are sent out Reach, grow fine, blade face is plump, no disease and pests harm.
The present invention seeks to be achieved through the following technical solutions:A kind of oil tea cuttage propagation method integrated with grafting, Comprise the following steps:
1) seedling medium preparation:Weigh following raw material by weight:Sandy loam 34-43 part, sawdust 11-14 part, vegetation Grey 11-17 part, chlorella powder 5-9 part, bagasse powder 17-23 part, compound fertilizer's 13-17 part, Oil-tea-cake powder 7-10 part, silkworm faeces 14- 18 parts, dipotassium hydrogen phosphate 7-11 part, zinc sulfate 5-8 part, potassium sulfate 6-8 part, calcium nitrate 5-8 part, ferrous sulfate 2-5 part, sulphuric acid Copper 3-7 part;Specifically preparation process is:After each raw material is fully mixed, heap is in heaps, adds water and mixes thoroughly, and amount of water is with substrate heap bottom Outflow water is not limited, natural compost more than 4 months, you can;
Described bagasse powder is to dry bagasse, is then comminuted into powder, and Particle size requirements are less than 1mm.
Preferably, following parts by weight composition pressed by each raw material of described seedling medium:37 parts of sandy loam, 13 parts of sawdust, vegetation 15 parts of ash, 7 parts of chlorella powder, 19 parts of bagasse powder, 15 parts of compound fertilizer, 8 parts of Oil-tea-cake powder, 15 parts of silkworm faeces, dipotassium hydrogen phosphate 8 Part, 7 parts of zinc sulfate, 6 parts of potassium sulfate, 5 parts of calcium nitrate, 4 parts of ferrous sulfate, 4 parts of copper sulfate.
2) load:The seedling medium that previous step is obtained is filled in nutritive cube, and is gently compacted, described nutritive cube specification For 12cm × 14cm;
3) move into seedbed:Select that sunny, irrigation and drainage are convenient, leeward place as nursery lot, to the nursery lot selected, Remove ground weeds, then start to make seedbed, seedbed width 1.1-1.2 rice, between seedbed and seedbed, excavate wide 40-50 centimetre, depth 8-10 centimetre of walkway, flattens seedbed face, after seedbed performs, by step 2) nutritive cube that loads full seedling medium moves into Seedling Bed, puts neat, and pours permeable, standby;
4) sterilize:Before cuttage, the potassium permanganate solution being 0.6-0.8 ‰ with concentration or 50% carbendazim wettable powder 600-800 times of aqueous solution of agent or the thiophanate methyl aqueous solution that concentration is 1-1.8 ‰ are carried out to the seedling medium in nutritive cube Spray disinfectant, fountain height, with the water seepage of nutritive cube bottom, sets up bamboo shelf immediately after, and covering thin film on bamboo shelf will Seedbed covers, thin film surrounding soil pressure tightening seal, Nausea drying 7-10 days, 1-2 days before cuttage, seedbed film hood two ends is opened, Allow cross-ventilation;
5) set up cool canopy:Nursery lot sets up cool canopy, cool canopy high 2.4-2.5 rice, and degree of shading is in 65%-75%;
6) cuttage bar is chosen and is processed:Take annual, semi-lignified, section upper band axillary bud, not damaged, the branch of no disease and pests harm By 1-2, one section is cut to axillary bud, long 10-12cm, as cuttage bar, cuttage base portion is cut -60 ° of tangent planes at 45 °, retain 1-2 pair Leaf, every leaf cuts off half, soaks in No. 1 or No. 2 rooting powder solutions of ABT training cuttage bar being put into 300-400mg/L Bubble 5-6 hour, taking-up is rinsed 5-8 minute with clear water, is ready for cuttage;
7) cuttage:Open the thin film of seedbed bamboo shelf, the seedling medium in nutritive cube is poured permeable, then cuttage bar is become In 45 ° of -60 ° of angles insertion seedling mediums, 1 plant of cuttage bar of each nutritive cube cuttage, insertion depth is with the 1/2 to 2/ of cuttage bar length 3 and retain leaf leaf base be higher than seedling medium distance be no less than 2 centimetres, cover thin film, surrounding after the completion of cuttage again Use soil pressure tightening seal;
8) nursery bed management after cuttage:The thin temperature in the shed in seedbed controls at 22-25 DEG C, humid control in 75-85%, by spray Watering is to ensure temperature and humidity;
9) grafting:10-15 days after cuttage, cuttage bar starts to extract out sprouting, forms calluss within 20-25 days, now, opens The thin film of seedbed bamboo shelf, cuttage bar is extracted, and is soaked with clear water and cleans, then takes out and carry out grafting, grafting using whip grafting Grafting fringe used selects high yield, the camellia oleifera cultivar of disease and insect resistance, and switch camellia oleifera cultivar is optimal, particularly Cenxi switch oil tea product Kind;Graft position at the above 3/5-2/3 of cuttage bar base portion, before cuttage bar carries out grafting or after the completion of grafting, on cuttage bar Sprouting pruned:Each sprouting branch retains 2-4 centimeter length, 1-2 opens new leaf, remaining excision;After the completion of grafting, by skewer It is molten that cutting base portion puts into No. 1 or No. 2 root-inducing powders of 100-200mg/L ABT containing 0.020mol/L left-handed cinnamic acid borneol acetate In liquid, soak 1.5 hours, then take out cuttage and return in seedling medium, spray water 15-20 minute, cover back thin film, surrounding soil pressure Tightening seal;
10) managing after grafting:The thin temperature in the shed in seedbed controls at 22-25 DEG C, and humid control is in 80-85%;After grafting from Second day starts, continuous 7 days, every morning 7-8 point, each watering of evening 7-8 point once, started 7-8 every night from the 8th day Once, to grafting fringe rudiment, irrigation times are changed to 3-4 days spill once afterwards for point watering, until grafting survival, high graft survival rate Reach more than 85%.
Carry out after grafting survival daily carried out 1 time every 30 days except eliminating pest, uprooting weed, meanwhile, carrying out sterilization and underground evil The preventing and treating of worm, until outplanting.
15-20 days after grafting survival, in addition to the sprouting that grafting fringe is extracted out retains the 1-2 rudiment branch growing fine, plant On remaining rudiment all erase.
Preferably, in this oil tea cuttage method for culturing seedlings integrated with grafting, in nursery bed management after cuttage, managing after grafting Watering is to install automatic spraying device by flexible pipe to realize, and watering every time is drenched with seedling medium top layer, and the time is general For 8-10 minute.
The solution of required preparation in the present invention, solute is insoluble in or water-fast, first with organic solvent such as ethanol dissolving Afterwards, then be diluted to desired concn.
Compared with prior art, the invention has the advantages that:
The method of the present invention, carries out cuttage, cuttage branch using the branch of wild camellia oleifera cultivar or non-prime camellia oleifera cultivar After forming calluss, in time cuttage branch is transferred to from seedling medium, transferred with high-quality camellia oleifera cultivar branch as stock Connect, then cuttage is returned in the seedling medium in nutritive cube again, carries out daily management.The method can be educated in large-scale production Seedling, is not subject to seasonal restrictions, and greatly reduces the demand to high-quality camellia oleifera cultivar branch again, solves high-quality oil tea variety source not The problem of foot.And growing-seedling period is short, graft survival rate up to more than 85%, can annual production nursery free of discontinuities, need not shift Nursery place, can be cultivated in nutritive cube till grafting survival Seedling goes out garden, easy to learn, low cost simple to operate, transfer Be connected into oil tea Seedling well developed root system alive, grow fine, blade face is plump, no disease and pests harm.
【Specific embodiment】
The following examples can help those skilled in the art that the present invention is more fully understood, but cannot be with any Mode limits the present invention.
Embodiment 1
A kind of oil tea cuttage propagation method integrated with grafting, comprises the following steps:
1) seedling medium preparation:Weigh following raw material by weight:34 parts of sandy loam, 11 parts of sawdust, 11 parts of plant ash, 5 parts of chlorella powder, 17 parts of bagasse powder, 13 parts of compound fertilizer, 7 parts of Oil-tea-cake powder, 14 parts of silkworm faeces, 7 parts of dipotassium hydrogen phosphate, zinc sulfate 5 parts, 6 parts of potassium sulfate, 5 parts of calcium nitrate, 2 parts of ferrous sulfate, 3 parts of copper sulfate;Specifically preparation process is:Each raw material is fully mixed Afterwards, heap is in heaps, adds water and mixes thoroughly, and with substrate heap bottom, outflow water is not limited amount of water, natural compost more than 4 months, you can;
2) load:The seedling medium that previous step is obtained is filled in nutritive cube, and is gently compacted, described nutritive cube specification For 12cm × 14cm;
3) move into seedbed:Select that sunny, irrigation and drainage are convenient, leeward place as nursery lot, to the nursery lot selected, Remove ground weeds, then start to make seedbed, seedbed width 1.1-1.2 rice, between seedbed and seedbed, excavate wide 40-50 centimetre, depth 8-10 centimetre of walkway, flattens seedbed face, after seedbed performs, by step 2) nutritive cube that loads full seedling medium moves into Seedling Bed, puts neat, and pours permeable, standby;
4) sterilize:Before cuttage, the potassium permanganate solution being 0.6-0.8 ‰ with concentration or 50% carbendazim wettable powder 600-800 times of aqueous solution of agent or the thiophanate methyl aqueous solution that concentration is 1-1.8 ‰ are carried out to the seedling medium in nutritive cube Spray disinfectant, fountain height, with the water seepage of nutritive cube bottom, sets up bamboo shelf immediately after, and covering thin film on bamboo shelf will Seedbed covers, thin film surrounding soil pressure tightening seal, Nausea drying 7-10 days, 1-2 days before cuttage, seedbed film hood two ends is opened, Allow cross-ventilation;
5) set up cool canopy:Nursery lot sets up cool canopy, cool canopy high 2.4-2.5 rice, and degree of shading is in 65%-75%;
6) cuttage bar is chosen and is processed:Take annual, semi-lignified, section upper band axillary bud, not damaged, the branch of no disease and pests harm By 1-2, one section is cut to axillary bud, long 10-12cm, as cuttage bar, cuttage base portion is cut -60 ° of tangent planes at 45 °, retain 1-2 pair Leaf, every leaf cuts off half, soaks 5 in No. 1 or No. 2 rooting powder solutions of ABT training cuttage bar being put into 300mg/L Hour, taking-up rinses 5-8 minute with clear water, is ready for cuttage;
7) cuttage:Open the thin film of seedbed bamboo shelf, the seedling medium in nutritive cube is poured permeable, then cuttage bar is become In 45 ° of -60 ° of angles insertion seedling mediums, 1 plant of cuttage bar of each nutritive cube cuttage, insertion depth is with the 1/2 to 2/ of cuttage bar length 3 and retain leaf leaf base be higher than seedling medium distance be no less than 2 centimetres, cover thin film, surrounding after the completion of cuttage again Use soil pressure tightening seal;
8) nursery bed management after cuttage:The thin temperature in the shed in seedbed controls at 22-25 DEG C, humid control in 75-85%, by spray Watering is to ensure temperature and humidity;
9) grafting:10-15 days after cuttage, cuttage bar starts to extract out sprouting, forms calluss within 20-25 days, now, opens The thin film of seedbed bamboo shelf, cuttage bar is extracted, and is soaked with clear water and cleans, then takes out and carry out grafting, grafting using whip grafting Grafting fringe used selects high yield, the camellia oleifera cultivar of disease and insect resistance, and switch camellia oleifera cultivar is optimal, particularly Cenxi switch oil tea product Kind;Graft position at the above 3/5-2/3 of cuttage bar base portion, before cuttage bar carries out grafting or after the completion of grafting, on cuttage bar Sprouting pruned:Each sprouting branch retains 2-4 centimeter length, 1-2 opens new leaf, remaining excision;After the completion of grafting, by skewer Cutting base portion is put in No. 1 or No. 2 rooting powder solutions of 100mg/L ABT containing 0.020mol/L left-handed cinnamic acid borneol acetate, Soak 1.5 hours, then take out cuttage and return in seedling medium, spray water 15-20 minute, cover back thin film, surrounding is tight with soil pressure Envelope;
10) managing after grafting:The thin temperature in the shed in seedbed controls at 22-25 DEG C, and humid control is in 80-85%;After grafting from Second day starts, continuous 7 days, every morning 7-8 point, each watering of evening 7-8 point once, started 7-8 every night from the 8th day Once, to grafting fringe rudiment, irrigation times are changed to 3-4 days spill once afterwards for point watering, until grafting survival, high graft survival rate Reach more than 85%.
Carry out after grafting survival daily carried out 1 time every 30 days except eliminating pest, uprooting weed, meanwhile, carrying out sterilization and underground evil The preventing and treating of worm, until outplanting.
15-20 days after grafting survival, in addition to the sprouting that grafting fringe is extracted out retains the 1-2 rudiment branch growing fine, plant On remaining rudiment all erase.
Wherein, nursery bed management after cuttage, the watering in managing after grafting are to install automatic spraying device by flexible pipe to realize , watering every time is drenched with seedling medium top layer, and the time is generally 8-10 minute.
Embodiment 2
A kind of oil tea cuttage propagation method integrated with grafting, comprises the following steps:
1) seedling medium preparation:Weigh following raw material by weight:37 parts of sandy loam, 13 parts of sawdust, 15 parts of plant ash, 7 parts of chlorella powder, 19 parts of bagasse powder, 15 parts of compound fertilizer, 8 parts of Oil-tea-cake powder, 15 parts of silkworm faeces, 8 parts of dipotassium hydrogen phosphate, zinc sulfate 7 parts, 6 parts of potassium sulfate, 5 parts of calcium nitrate, 4 parts of ferrous sulfate, 4 parts of copper sulfate;Specifically preparation process is:Each raw material is fully mixed Afterwards, heap is in heaps, adds water and mixes thoroughly, and with substrate heap bottom, outflow water is not limited amount of water, natural compost more than 4 months, you can;
2) load:The seedling medium that previous step is obtained is filled in nutritive cube, and is gently compacted, described nutritive cube specification For 12cm × 14cm;
3) move into seedbed:Select that sunny, irrigation and drainage are convenient, leeward place as nursery lot, to the nursery lot selected, Remove ground weeds, then start to make seedbed, seedbed width 1.1-1.2 rice, between seedbed and seedbed, excavate wide 40-50 centimetre, depth 8-10 centimetre of walkway, flattens seedbed face, after seedbed performs, by step 2) nutritive cube that loads full seedling medium moves into Seedling Bed, puts neat, and pours permeable, standby;
4) sterilize:Before cuttage, the potassium permanganate solution being 0.6-0.8 ‰ with concentration or 50% carbendazim wettable powder 600-800 times of aqueous solution of agent or the thiophanate methyl aqueous solution that concentration is 1-1.8 ‰ are carried out to the seedling medium in nutritive cube Spray disinfectant, fountain height, with the water seepage of nutritive cube bottom, sets up bamboo shelf immediately after, and covering thin film on bamboo shelf will Seedbed covers, thin film surrounding soil pressure tightening seal, Nausea drying 7-10 days, 1-2 days before cuttage, seedbed film hood two ends is opened, Allow cross-ventilation;
5) set up cool canopy:Nursery lot sets up cool canopy, cool canopy high 2.4-2.5 rice, and degree of shading is in 65%-75%;
6) cuttage bar is chosen and is processed:Take annual, semi-lignified, section upper band axillary bud, not damaged, the branch of no disease and pests harm By 1-2, one section is cut to axillary bud, long 10-12cm, as cuttage bar, cuttage base portion is cut -60 ° of tangent planes at 45 °, retain 1-2 pair Leaf, every leaf cuts off half, soaks 6 in No. 1 or No. 2 rooting powder solutions of ABT training cuttage bar being put into 350mg/L Hour, taking-up rinses 5-8 minute with clear water, is ready for cuttage;
7) cuttage:Open the thin film of seedbed bamboo shelf, the seedling medium in nutritive cube is poured permeable, then cuttage bar is become In 45 ° of -60 ° of angles insertion seedling mediums, 1 plant of cuttage bar of each nutritive cube cuttage, insertion depth is with the 1/2 to 2/ of cuttage bar length 3 and retain leaf leaf base be higher than seedling medium distance be no less than 2 centimetres, cover thin film, surrounding after the completion of cuttage again Use soil pressure tightening seal;
8) nursery bed management after cuttage:The thin temperature in the shed in seedbed controls at 22-25 DEG C, humid control in 75-85%, by spray Watering is to ensure temperature and humidity;
9) grafting:10-15 days after cuttage, cuttage bar starts to extract out sprouting, forms calluss within 20-25 days, now, opens The thin film of seedbed bamboo shelf, cuttage bar is extracted, and is soaked with clear water and cleans, then takes out and carry out grafting, grafting using whip grafting Grafting fringe used selects high yield, the camellia oleifera cultivar of disease and insect resistance, and switch camellia oleifera cultivar is optimal, particularly Cenxi switch oil tea product Kind;Graft position at the above 3/5-2/3 of cuttage bar base portion, before cuttage bar carries out grafting or after the completion of grafting, on cuttage bar Sprouting pruned:Each sprouting branch retains 2-4 centimeter length, 1-2 opens new leaf, remaining excision;After the completion of grafting, by skewer Cutting base portion is put in No. 1 or No. 2 rooting powder solutions of 150mg/L ABT containing 0.020mol/L left-handed cinnamic acid borneol acetate, Soak 1.5 hours, then take out cuttage and return in seedling medium, spray water 15-20 minute, cover back thin film, surrounding is tight with soil pressure Envelope;
10) managing after grafting:The thin temperature in the shed in seedbed controls at 22-25 DEG C, and humid control is in 80-85%;After grafting from Second day starts, continuous 7 days, every morning 7-8 point, each watering of evening 7-8 point once, started 7-8 every night from the 8th day Once, to grafting fringe rudiment, irrigation times are changed to 3-4 days spill once afterwards for point watering, until grafting survival, high graft survival rate Reach more than 85%.
Carry out after grafting survival daily carried out 1 time every 30 days except eliminating pest, uprooting weed, meanwhile, carrying out sterilization and underground evil The preventing and treating of worm, until outplanting.
15-20 days after grafting survival, in addition to the sprouting that grafting fringe is extracted out retains the 1-2 rudiment branch growing fine, plant On remaining rudiment all erase.
Wherein, nursery bed management after cuttage, the watering in managing after grafting are to install automatic spraying device by flexible pipe to realize , watering every time is drenched with seedling medium top layer, and the time is generally 8-10 minute.
Embodiment 3
A kind of oil tea cuttage propagation method integrated with grafting, comprises the following steps:
1) seedling medium preparation:Weigh following raw material by weight:43 parts of sandy loam, 14 parts of sawdust, 17 parts of plant ash, 9 parts of chlorella powder, 23 parts of bagasse powder, 17 parts of compound fertilizer, 10 parts of Oil-tea-cake powder, 18 parts of silkworm faeces, 11 parts of dipotassium hydrogen phosphate, sulphuric acid 8 parts of zinc, 8 parts of potassium sulfate, 8 parts of calcium nitrate, 5 parts of ferrous sulfate, 7 parts of copper sulfate;Specifically preparation process is:Will be fully mixed for each raw material After even, heap is in heaps, adds water and mixes thoroughly, and with substrate heap bottom, outflow water is not limited amount of water, natural compost 5 months, you can;
2) load:The seedling medium that previous step is obtained is filled in nutritive cube, and is gently compacted, described nutritive cube specification For 12cm × 14cm;
3) move into seedbed:Select that sunny, irrigation and drainage are convenient, leeward place as nursery lot, to the nursery lot selected, Remove ground weeds, then start to make seedbed, seedbed width 1.1-1.2 rice, between seedbed and seedbed, excavate wide 40-50 centimetre, depth 8-10 centimetre of walkway, flattens seedbed face, after seedbed performs, by step 2) nutritive cube that loads full seedling medium moves into Seedling Bed, puts neat, and pours permeable, standby;
4) sterilize:Before cuttage, the potassium permanganate solution being 0.6-0.8 ‰ with concentration or 50% carbendazim wettable powder 600-800 times of aqueous solution of agent or the thiophanate methyl aqueous solution that concentration is 1-1.8 ‰ are carried out to the seedling medium in nutritive cube Spray disinfectant, fountain height, with the water seepage of nutritive cube bottom, sets up bamboo shelf immediately after, and covering thin film on bamboo shelf will Seedbed covers, thin film surrounding soil pressure tightening seal, Nausea drying 7-10 days, 1-2 days before cuttage, seedbed film hood two ends is opened, Allow cross-ventilation;
5) set up cool canopy:Nursery lot sets up cool canopy, cool canopy high 2.4-2.5 rice, and degree of shading is in 65%-75%;
6) cuttage bar is chosen and is processed:Take annual, semi-lignified, section upper band axillary bud, not damaged, the branch of no disease and pests harm By 1-2, one section is cut to axillary bud, long 10-12cm, as cuttage bar, cuttage base portion is cut -60 ° of tangent planes at 45 °, retain 1-2 pair Leaf, every leaf cuts off half, soaks 5 in No. 1 or No. 2 rooting powder solutions of ABT training cuttage bar being put into 400mg/L Hour, taking-up rinses 5-8 minute with clear water, is ready for cuttage;
7) cuttage:Open the thin film of seedbed bamboo shelf, the seedling medium in nutritive cube is poured permeable, then cuttage bar is become In 45 ° of -60 ° of angles insertion seedling mediums, 1 plant of cuttage bar of each nutritive cube cuttage, insertion depth is with the 1/2 to 2/ of cuttage bar length 3 and retain leaf leaf base be higher than seedling medium distance be no less than 2 centimetres, cover thin film, surrounding after the completion of cuttage again Use soil pressure tightening seal;
8) nursery bed management after cuttage:The thin temperature in the shed in seedbed controls at 22-25 DEG C, humid control in 75-85%, by spray Watering is to ensure temperature and humidity;
9) grafting:10-15 days after cuttage, cuttage bar starts to extract out sprouting, forms calluss within 20-25 days, now, opens The thin film of seedbed bamboo shelf, cuttage bar is extracted, and is soaked with clear water and cleans, then takes out and carry out grafting, grafting using whip grafting Grafting fringe used selects high yield, the camellia oleifera cultivar of disease and insect resistance, and switch camellia oleifera cultivar is optimal, particularly Cenxi switch oil tea product Kind;Graft position at the above 3/5-2/3 of cuttage bar base portion, before cuttage bar carries out grafting or after the completion of grafting, on cuttage bar Sprouting pruned:Each sprouting branch retains 2-4 centimeter length, 1-2 opens new leaf, remaining excision;After the completion of grafting, by skewer Cutting base portion is put in No. 1 or No. 2 rooting powder solutions of 200mg/L ABT containing 0.020mol/L left-handed cinnamic acid borneol acetate, Soak 1.5 hours, then take out cuttage and return in seedling medium, spray water 15-20 minute, cover back thin film, surrounding is tight with soil pressure Envelope;
10) managing after grafting:The thin temperature in the shed in seedbed controls at 22-25 DEG C, and humid control is in 80-85%;After grafting from Second day starts, continuous 7 days, every morning 7-8 point, each watering of evening 7-8 point once, started 7-8 every night from the 8th day Once, to grafting fringe rudiment, irrigation times are changed to 3-4 days spill once afterwards for point watering, until grafting survival, high graft survival rate Reach more than 85%.
Carry out after grafting survival daily carried out 1 time every 30 days except eliminating pest, uprooting weed, meanwhile, carrying out sterilization and underground evil The preventing and treating of worm, until outplanting.
15-20 days after grafting survival, in addition to the sprouting that grafting fringe is extracted out retains the 1-2 rudiment branch growing fine, plant On remaining rudiment all erase.
Wherein, nursery bed management after cuttage, the watering in managing after grafting are to install automatic spraying device by flexible pipe to realize , watering every time is drenched with seedling medium top layer, and the time is generally 8-10 minute.
15-20 days after grafting survival, the survival rate of oil tea grafting, number of averagely taking root, root average length, sprouting are put down All base diameter, sprouting average length are counted, the statistical data such as table 1 of embodiment 1-3.
Based on the oil tea cuttage of embodiment 1-3 method for culturing seedlings integrated with grafting, do contrast test.
In the case that other steps are constant, step 9) in, do not contain left-handed Cortex Cinnamomi in No. 1 or No. 2 rooting powder solutions of ABT Sour borneol acetate, tests as a comparison, the corresponding contrast test of embodiment 1-3 1, contrast test 2, the statistical data of contrast test 3 As table 2.
In the case that other steps are constant, step 9) in, after the completion of grafting, cuttage bar base portion is put into 0.020mol/L In left-handed cinnamic acid Borneolum Syntheticum ester solution, soak 1.5 hours, then take out cuttage and return in seedling medium, test as a comparison, implement The example corresponding contrast test 1 ' of 1-3, contrast test 2 ', the statistical data such as table 3 of contrast test 3 '.
Table 1
Table 2
Table 3
Based on the oil tea cuttage of embodiment 2 method for culturing seedlings integrated with grafting, do confirmatory experiment.
In the case that other steps are constant, step 9) in, contain in No. 1 or No. 2 rooting powder solutions of 150mg/L ABT Left-handed cinnamic acid borneol acetate concentration be respectively 0.005mol/L, 0.010mol/L, 0.015mol/L, 0.025mol/L, 0.030mol/L, 0.035mol/L, as confirmatory experiment 1.
15-20 days after grafting survival, the survival rate of oil tea grafting, number of averagely taking root, root average length, sprouting are put down All base diameter, sprouting average length are counted, statistical data such as table 4.
In the case that other steps are constant, step 9) in, the concentration of No. 1 or No. 2 rooting powder solution of ABT is respectively 50mg/L, 80mg/L, 100mg/L, 180mg/L, 200mg/L, 250mg/L, as confirmatory experiment 2.
15-20 days after grafting survival, the survival rate of oil tea grafting, number of averagely taking root, root average length, sprouting are put down All base diameter, sprouting average length are counted, statistical data such as table 5.
Table 4
Table 5
From above-mentioned table 1, table 2, table 3, table 4, table 5 statistical data, after the completion of grafting, cuttage bar base portion is put into and contains Have in the 150mg/L rooting powder solution of 0.020mol/L left-handed cinnamic acid borneol acetate, soak 1.5 hours, then nursery base is returned in cuttage In matter, to the survival rate of oil tea grafting, averagely take root number, root average length, the average base diameter of sprouting, sprouting average length Impact is maximum, and the data of acquisition is best.
Although, above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause This, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.

Claims (9)

1. a kind of oil tea cuttage propagation method integrated with grafting is it is characterised in that the method comprising the steps of:
1) seedling medium preparation:Weigh following raw material by weight:Sandy loam 34-43 part, sawdust 11-14 part, plant ash 11- 17 parts, chlorella powder 5-9 part, bagasse powder 17-23 part, compound fertilizer's 13-17 part, Oil-tea-cake powder 7-10 part, silkworm faeces 14-18 part, Dipotassium hydrogen phosphate 7-11 part, zinc sulfate 5-8 part, potassium sulfate 6-8 part, calcium nitrate 5-8 part, ferrous sulfate 2-5 part, copper sulfate 3-7 Part;Specifically preparation process is:After each raw material is fully mixed, heap is in heaps, adds water and mixes thoroughly, and amount of water is not flowed out with substrate heap bottom Water is limited, natural compost more than 4 months, you can;
2) load:The seedling medium that previous step is obtained is filled in nutritive cube, and is gently compacted, and described nutritive cube specification is 12cm×14cm;
3) move into seedbed:Selection is sunny, irrigation and drainage are convenient, leeward place is as nursery lot, the nursery lot to selection, removing Ground weeds, then start to make seedbed, seedbed width 1.1-1.2 rice, excavate wide 40-50 centimetre, deep 8-10 between seedbed and seedbed Centimetre walkway, flatten seedbed face, after seedbed performs, by step 2) nutritive cube that loads full seedling medium moves into seedbed, pendulum Put neat, and pour permeable, standby;
4) sterilize:Before cuttage, the potassium permanganate solution being 0.6-0.8 ‰ with concentration or 50% carbendazol wettable powder 600-800 times of aqueous solution or the thiophanate methyl aqueous solution that concentration is 1-1.8 ‰ spray to the seedling medium in nutritive cube Spill sterilization, fountain height, with the water seepage of nutritive cube bottom, sets up bamboo shelf immediately after, covers thin film by Seedling on bamboo shelf Bed covers, thin film surrounding soil pressure tightening seal, and Nausea drying 7-10 days, 1-2 days before cuttage, seedbed film hood two ends is opened, is allowed Cross-ventilation;
5) set up cool canopy:Nursery lot sets up cool canopy, cool canopy high 2.4-2.5 rice, and degree of shading is in 65%-75%;
6) cuttage bar is chosen and is processed:Take annual, semi-lignified, section upper band axillary bud, not damaged, the branch of no disease and pests harm by 1- 2 couples of axillary buds, long 10-12cm are cut to one section, as cuttage bar, cuttage base portion are cut -60 ° of tangent planes at 45 °, retain 1-2 to leaf, Every leaf cuts off half, soaks 5-6 in No. 1 or No. 2 rooting powder solutions of ABT training cuttage bar being put into 300-400mg/L Hour, taking-up rinses 5-8 minute with clear water, is ready for cuttage;
7) cuttage:Open the thin film of seedbed bamboo shelf, the seedling medium in nutritive cube poured permeable, then by 45 ° for cuttage bar- In 60 ° of angles insertion seedling mediums, 1 plant of cuttage bar of each nutritive cube cuttage, insertion depth with the 1/2 to 2/3 of cuttage bar length and And the leaf base of reservation leaf is no less than 2 centimetres higher than the distance of seedling medium, after the completion of cuttage, cover thin film, surrounding soil again Compression sealing;
8) nursery bed management after cuttage:The thin temperature in the shed in seedbed controls at 22-25 DEG C, humid control in 75-85%, by spray water To ensure temperature and humidity;
9) grafting:10-15 days after cuttage, cuttage bar starts to extract out sprouting, forms calluss within 20-25 days, cuttage survival rate is high Reach more than 95%, now, open the thin film of seedbed bamboo shelf, cuttage bar is extracted, soaked with clear water and clean, then take out employing Whip grafting carries out grafting, and graft position, at the above 3/5-2/3 of cuttage bar base portion, after the completion of grafting, cuttage bar base portion is put into In No. 1 or No. 2 rooting powder solutions of 100-200mg/L ABT containing 0.020mol/L left-handed cinnamic acid borneol acetate, soak 1.5 Hour, then take out cuttage and return in seedling medium, spray water 15-20 minute, cover back thin film, surrounding soil pressure tightening seal;
10) managing after grafting:The thin temperature in the shed in seedbed controls at 22-25 DEG C, and humid control is in 80-85%;From second after grafting It starts, continuous 7 days, every morning 7-8 point, each watering of evening 7-8 point once, started 7-8 point every night from the 8th day and spill Once, to grafting fringe rudiment, irrigation times are changed to 3-4 days spill once water afterwards, until grafting survival.
2. oil tea cuttage as claimed in claim 1 method for culturing seedlings integrated with grafting it is characterised in that:Described seedling medium is each Following parts by weight composition pressed by raw material:37 parts of sandy loam, 13 parts of sawdust, 15 parts of plant ash, 7 parts of chlorella powder, bagasse powder 19 Part, 15 parts of compound fertilizer, 8 parts of Oil-tea-cake powder, 15 parts of silkworm faeces, 8 parts of dipotassium hydrogen phosphate, 7 parts of zinc sulfate, 6 parts of potassium sulfate, calcium nitrate 5 Part, 4 parts of ferrous sulfate, 4 parts of copper sulfate.
3. oil tea cuttage as claimed in claim 1 method for culturing seedlings integrated with grafting it is characterised in that:Step 9) in grafting, Before cuttage bar carries out grafting or after the completion of grafting, the sprouting on cuttage bar is pruned:Each sprouting branch retains 2-4 centimetre Long, 1-2 opens new leaf, remaining excision.
4. oil tea cuttage as claimed in claim 1 method for culturing seedlings integrated with grafting it is characterised in that:Step 9) in grafting, Grafting fringe used by grafting selects high yield, disease and insect resistance camellia oleifera cultivar.
5. oil tea cuttage as claimed in claim 4 method for culturing seedlings integrated with grafting it is characterised in that:Described camellia oleifera cultivar is Switch camellia oleifera cultivar.
6. oil tea cuttage as claimed in claim 5 method for culturing seedlings integrated with grafting it is characterised in that:Described switch oil tea product Planting is Cenxi switch oil tea.
7. oil tea cuttage as claimed in claim 1 method for culturing seedlings integrated with grafting it is characterised in that:Step 10) after grafting In management, 15-20 days after grafting survival, in addition to the sprouting that grafting fringe is extracted out retains the 1-2 rudiment branch growing fine, on plant Remaining rudiment all erase.
8. oil tea cuttage as claimed in claim 1 method for culturing seedlings integrated with grafting it is characterised in that:Step 10) after grafting In management, carry out after grafting survival daily carried out 1 time every 30 days except eliminating pest, uprooting weed, meanwhile, carrying out sterilization and underground evil The preventing and treating of worm, until outplanting.
9. oil tea cuttage as claimed in claim 1 method for culturing seedlings integrated with grafting it is characterised in that:Nursery pipe after cuttage Watering in reason, managing after grafting is to install automatic spraying device by flexible pipe to realize.
CN201610743664.6A 2016-08-27 2016-08-27 Tea-oil tree cutting and grafting integral reproduction method Pending CN106386184A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106962028A (en) * 2017-05-17 2017-07-21 佛山市林业科学研究所 Camellia cuttage grafting integration quick-breeding method
CN109005980A (en) * 2018-07-18 2018-12-18 安徽德昌苗木有限公司 A kind of grafting method of camellia bud stocks
CN109924101A (en) * 2019-04-16 2019-06-25 湖南省林大油茶有限公司 A kind of Camellia oleifera Cutting Seedling Raising matrix and preparation method thereof
CN111512942A (en) * 2020-05-13 2020-08-11 青阳县三三富林中药材有限责任公司 Polygonatum sibiricum asexual propagation method for improving emergence rate and strong seedling rate

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CN104303770A (en) * 2014-10-13 2015-01-28 金华市永根杜鹃花培育有限公司 Seedling scion grafting method
CN105210635A (en) * 2015-10-13 2016-01-06 广西乐业荣丰中药材科技有限责任公司 A kind of honeysuckle cuttage and seedling culture method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104303770A (en) * 2014-10-13 2015-01-28 金华市永根杜鹃花培育有限公司 Seedling scion grafting method
CN105210635A (en) * 2015-10-13 2016-01-06 广西乐业荣丰中药材科技有限责任公司 A kind of honeysuckle cuttage and seedling culture method

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106962028A (en) * 2017-05-17 2017-07-21 佛山市林业科学研究所 Camellia cuttage grafting integration quick-breeding method
CN106962028B (en) * 2017-05-17 2020-07-03 佛山市林业科学研究所 Camellia cuttage grafting integrated rapid propagation method
CN109005980A (en) * 2018-07-18 2018-12-18 安徽德昌苗木有限公司 A kind of grafting method of camellia bud stocks
CN109924101A (en) * 2019-04-16 2019-06-25 湖南省林大油茶有限公司 A kind of Camellia oleifera Cutting Seedling Raising matrix and preparation method thereof
CN111512942A (en) * 2020-05-13 2020-08-11 青阳县三三富林中药材有限责任公司 Polygonatum sibiricum asexual propagation method for improving emergence rate and strong seedling rate

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