CN106171716A - Floral leaf Buxus sempervirens/common box cuttage breeding method - Google Patents

Abstract

Floral leaf Buxus sempervirens/common box cuttage breeding method, belongs to technical field of plant propagation.Comprise the following steps: 1) choose suitable breeding spot and set up floral leaf Buxus sempervirens/common box female parent cutting orchard；2) 72 hole dish cuttages are used；3) suitable cutting time is early September, cuttage in 1 year 1 time；4) cuttage branch uses and urges root agent to impregnate；5) after cuttage 25 days, the fringe bar root of hair of about 90% can start seedling exercising.Above-mentioned floral leaf Buxus sempervirens/common box cuttage breeding method, simple to operate, reproduction speed is fast, and yield rate is up to 90%, for domestic reported first, fills up the blank of correlative technology field, can be used for large-scale production, favorable repeatability, has good market prospect.

Description

Floral leaf Buxus sempervirens/common box cuttage breeding method

Technical field

The invention belongs to technical field of plant propagation, specially floral leaf Buxus sempervirens/common box cuttage breeding method.

Background technology

Floral leaf Buxus sempervirens/common box Buxus sempervirens ' Variegata ', Buxaceae, Buxus, evergreen broad-leaved fills Wood, plant height 0.5-1.5m, it is preced with width 0.4-1.2m, tree-like compact, branch is dense, uprightly stretches, and bark Lycoperdon polymorphum Vitt is to olive colour, ripe Time irregularly split.Dan Ye, to life, leaf keratin, smooth, elliptic leaf shape is the most avette, long avette, pinnate vein, Quan Yuan, base portion wedge Shape, top is tapering；Leaf mottle is refuted various, bright green, and leaf margin tool width irregular white annulus, this annulus gradually turned with season For milky, it is finally light yellow, tool sight；If meeting low temperature in some areas, partial blade becomes bronze or brown pale brown Color, particularly after high light direct projection and strong wind, sight is affected.Floral white, fascicled leaves axil or branch top；Male flower is born in flower , there is sepal 4 side of sequence；Female flower top is raw, has sepal 6；Ovary 3 Room, there is ovule 2 every room；The month at florescence 4-5.Capsule, 3 lobes split, really The top of lobe has 2 jiaos.Happiness light, the most resistance to shade, can grow under the conditions of full exposure, part are sheltered from heat or light, more like mottled sunlight；Happiness Moderate amount of moisture, fertile well-drained sandy loam, adapt to subacidity, neutrality and alkalescence soil, it is possible at sandy soil and glue Normal growth in soil, the speed of growth is slower；Resistance to pruning；Shallow root, therefore plant next-door neighbour's surrounding can not be turned over；Remove it in good time Bad, dead branch, leaf, to promote that the growing environment air of plant circulates；Avoid the strong wind of cold winter as far as possible；It is weak to accumulated snow. ' floral leaf ' Buxus sempervirens/common box originates in Europe, and its original seed Buxus sempervirens/common box (Buxus sempervirens) is distributed in central China, various places Extensively cultivation.Luo Fengxia etc. (1994) have carried out systematic research to the cuttage breeding method of Buxus sempervirens/common box, and result shows spring Cuttage survival rate is up to more than 87%, and summer, cuttage survival rate was up to more than 94%.Sun Jing etc. (2005) report gold Leaf Buxus sempervirens/common box cuttage raise seedling technique, cuttage survival rate can be more than 90%.But existing document the most not to cutting orchard set up and Commercial seedling is explained in detail.And consult available data, there is no the relevant report of floral leaf Buxus sempervirens/common box.2007, Zhejiang Sen Hezhong industry limited company introduces floral leaf Buxus sempervirens/common box from Japan, through safety, regionality, compatibility test, and adopts Root agent, variable concentrations, different substrates formula and the test of cottage method is urged, it is thus achieved that success with different plants.Subject range: peace Emblem, Jiangsu, Zhejiang, Jiangxi, Hunan, Hubei, Sichuan, Guizhou, Yunnan.Can sheet plant, group planting, avenue system, the product of making have color lump, Spherical and short hedge, plants by garden, park, river, beach, is excellent floral leaf series vegetable, has wide market prospect.

Summary of the invention

For the above-mentioned problems in the prior art, it is an object of the invention to design provides a kind of floral leaf Buxus sempervirens/common box The technical scheme of cuttage breeding method, comprises and sets up cutting orchard, and it is simple to operate, reproduction speed is fast, and yield rate is up to 90%.

Described floral leaf Buxus sempervirens/common box cuttage breeding method, it is characterised in that comprise the following steps:

1) choosing suitable breeding spot and set up floral leaf Buxus sempervirens/common box female parent cutting orchard, container seedling transplants seeding row spacing for transplanting seeding row spacing For 25cm × 35cm, density is 4000-5000 plant/acre；

2) using 72 hole dish cuttages, keep substrate elasticity consistent, described substrate contains the following material of weight percentage: Peat 30-40%, furfural dregs 5-10%, perlite 20-30%, fine sand 10-20%, straw 10-20%, Herba Agerati Conyzoidis ground product 3-7%, purple Rowland stem and leaf ground product 2-4%；

3) suitable cutting time is early September, and cuttage in 1 year 1 time, cuttings length is 2-3cm, retains 2 lateral buds, 2 opposite leaves Sheet, base portion flat nick；

4) cuttage branch uses and urges root agent 500-600ppm dipping fringe bar base portion 2-3 second, described in urge root agent be 3-by weight ratio The naphthalene acetic acid of 5:3-5:1:1: heteroauxing: antisepsis peptide of wilted hay bacilli: the mixing of chrysanthemum total flavone extracting solution is constituted；

5) the cuttage degree of depth is 2-3cm, waters permeable after cuttage immediately, sprays the carbendazim of 600-700 times of liquid, arch after the airing of blade face Canopy is cultivated, and in canopy, air humidity reaches more than 90%, after cuttage 10 days, starts spray foliage fertilizer for the first time, later every 7-10 days, Watering and spray 1 time afterwards, foliage fertilizer can spray with green fragrant 900-1000 times of liquid of Wei, simultaneously with 900-1000 times of liquid Mancozeb spray Mist diseases prevention, after cuttage 25 days, the fringe bar root of hair of about 90% can start seedling exercising.

Described floral leaf Buxus sempervirens/common box cuttage breeding method, it is characterised in that in step 1):

The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed, Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower；Pavement width 40cm, deeply 25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded；

March in early November to next year transplants, and transplanting seeding row spacing is 25cm × 35cm, and density is 5000 plants/acre, and fixed point is dug Cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant, be compacted in root with fine earth heap, in time during transplanting Irrigating and determine root water, bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth；

Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later；In case of even The continuous rainy day, timely draining；Full exposure is cultivated, and promotes fringe bar lignifying；Within after transplanting 15 days, starting fertilising, the 3-8 month is every half The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 15kg, stops executing quick-acting fertilizer after JIUYUE, executes become thoroughly decomposed organic the 11-12 month 1 time Fertilizer, every mu of 1500kg, buriing of ditching, when spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, with no seedling injury Wood stem, branch and blade are as the criterion；After adopting fringe, execute 1 quick-acting fertilizer, every mu of 20kg immediately.

Described floral leaf Buxus sempervirens/common box cuttage breeding method, it is characterised in that step 2) described in substrate contain weight percent The following material of content: peat 32-35%, furfural dregs 6-8%, perlite 25-28%, fine sand 12-18%, straw 12-15%, win red Ji ground product 4-5%, violet stem and leaf ground product 3%.

Described floral leaf Buxus sempervirens/common box cuttage breeding method, it is characterised in that step 2) described in cutting medium use with Prepared by lower section method:

1) pretreatment: red Ji, violet stem and leaf are dried, are pulverized, prepares red Ji ground product, violet stem and leaf ground product；

2) by the following material mix homogeneously of weight percentage: peat 30-40%, furfural dregs 5-10%, perlite 20-30%, thin Husky 10-20%, straw 10-20%, Herba Agerati Conyzoidis ground product 3-7%, violet stem and leaf ground product 2-4%.

Described floral leaf Buxus sempervirens/common box cuttage breeding method, it is characterised in that the chrysanthemum total flavone described in step 4) extracts Liquid uses following methods to prepare:

1) Flos Chrysanthemi pulverizing is broken into coarse powder, cross 30 mesh sieves；

2) seepage pressure effects: Flos Chrysanthemi coarse powder is loaded in percolator, after soaking 10 hours with 75% aquiferous ethanol, use 70% aquiferous ethanol Carrying out seepage pressure effects, if raw material weight is 1kg, the consumption of aquiferous ethanol is 16 liters, and flow velocity is 5mL/min, collects percolate；

3) flash concentration: by percolate bath temperature 80 DEG C, vacuum is 0.095 Mpa, and feed liquor flow velocity is 300mL min^-1Under conditions of carry out vacuum film concentration, reclaim ethanol, obtain every mL medicinal liquid and convert into the concentrated solution containing 2.0g raw material；

4) macroporous resin adsorption purification: by the concentrated solution that obtains by macroporous adsorbent resin separation and concentration, successively with water, 30% contain Water-ethanol, 40% aquiferous ethanol, 50% aquiferous ethanol, 60% aquiferous ethanol, 70% aquiferous ethanol carry out gradient elution, finally with 70% Acetone eluting, respectively collect 3 times of column volumes each position eluent, collect merge 40% aquiferous ethanol, 50% aquiferous ethanol, 60% The eluent at each position of aquiferous ethanol, is concentrated into the 1/4 of original volume by the eluent of merging, i.e. obtains chrysanthemum total flavone and extracts Liquid.

Described floral leaf Buxus sempervirens/common box cuttage breeding method, it is characterised in that in step 4): naphthalene acetic acid: heteroauxing: withered Grass bacillus cereus antibacterial peptide: the weight ratio of chrysanthemum total flavone extracting solution is 4:4:1:1.

Herba Agerati Conyzoidis, wild feverfew, originate in american torrid zone.Leaf oval, fissure, to life, spend blueness, pink, Lavender or white, dense cluster is raw；Achene is little.The dwarfing in common flower garden Herba Agerati Conyzoidis (A. houstonianum, i.e. Ageratum houstonianum Miller.) Mutation is planted as edgewise plant.Herb frowziness, medicinal, heat-clearing and toxic substances removing, detumescence hemostasis, also there is stronger insecticidal effect.Above-mentioned Herba Agerati Conyzoidis ground product in substrate, has disinsection efficiency, is mixed and accompanies in substrate, slow release, long-acting pesticidal, the raw material of employing Come from nature, belong to botanical pesticide, do not develop immunity to drugs, use safety.

Violet, originates in Mediterranean, and the extensively cultivation of current south China area, under Cruciferae Matthiola incana The general designation of the plant of each kind.Violet stem and leaf ground product in above-mentioned cutting medium, plays and helps root effect.

Furfural dregs in above-mentioned substrate is, after the agricultural byproducts such as corncob, bagasse are pulverized, a series of hydrolyzed chemical occurs Remaining waste residue after furfural is extracted in reaction, and its rich cellulose, protein, moisture content and the porosity of furfural dregs are the highest, say Its loose, porous bright, has good adsorptivity, improves the permeability of substrate.Open from nutritional labeling, the nitrogen of furfural dregs, phosphorus, Potassium and organic content are higher, are conducive to improving the fertility state of substrate.

Straw in above-mentioned substrate is pulverized 2-4cm respectively by Semen arachidis hypogaeae bar, bean bar, and adjustment water content 55-70%, C/N compare 30- 32, it is piled into ridge, with covered rearing with plastic film, makes the abundant fermentation maturity of straw, respectively obtain become thoroughly decomposed Semen arachidis hypogaeae bar and the bean bar that becomes thoroughly decomposed, will Becoming thoroughly decomposed Semen arachidis hypogaeae bar and the ratio of the bean bar 2:1 that becomes thoroughly decomposed is mixed to prepare, have establishing in large scale in China, once, resource is rich for annual results Rich.

The cutting medium reasonable raw material proportioning that the present invention uses, environmentally safe, the nutrient of integrated substrate enriches, trace Nutrient is complete, and cuttage shoot growth amount is had significant facilitation.

The above-mentioned antisepsis peptide of wilted hay bacilli urged in root agent, the antibacterial peptide matters extracted from antibacterial, is microorganism Fermenting natural, active height, pollutes few, and the feature such as not accumulation, can stop the normal of pathogenic microorganism in the environment Metabolic pathway or kill pathogenic microorganism and reach the purpose of controlling disease.

Urge addition chrysanthemum total flavone extracting solution in root agent above-mentioned, the rooting rate of cuttage branch can be remarkably promoted, and improve it Disease resistance.Containing substantial amounts of flavone compound in chrysanthemum total flavone extracting solution, natural flavone compounds, without any chemistry Synthesis class material, it can promote microorganism, plant cellular material operating and accumulation and cell enzyme activity, and then improve useful micro-life The metabolic capacity of thing bacterium, increases the disease resistance of plant, promotes plant growing.

In the present invention, cutting orchard is set up and management, cutting time, cuttings length, cutting medium form and quality, urge root agent Concentration and formula is different, yield rate is all had a significant impact by management after cuttage.The each component of cutting medium, proportioning have collaborative Potentiation.Each component, proportioning in root agent is urged also to have synergistic function.

Described peat, furfural dregs, perlite, fine sand, straw, Herba Agerati Conyzoidis ground product, violet stem and leaf ground product, naphthalene second Acid, heteroauxing, antisepsis peptide of wilted hay bacilli, chrysanthemum total flavone extracting solution, green fragrant Wei, Mancozeb all can be from the market Directly buy.

Above-mentioned floral leaf Buxus sempervirens/common box cuttage breeding method, simple to operate, reproduction speed is fast, and yield rate is up to 90%, for domestic Reported first, fills up the blank of correlative technology field, can be used for large-scale production, favorable repeatability, before having good market Scape.

The percentage composition related in present specification unless otherwise indicated, refers both to weight percentage.

Detailed description of the invention

In conjunction with embodiments of the invention, the invention will be further described.

1 cutting orchard is set up and management

1.1 ground, gardens

The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed, Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower；Pavement width 40cm, deeply 25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded.

1.2 transplant

March in early November to next year transplants.Graphic by seeding row spacing design configurations, transplanting seeding row spacing is 25cm × 35cm, close Degree is 5000 plants/acre.Fixed point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant during transplanting, with thin Mound is compacted in root, irrigates in time and determines root water, and bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth.

1.3 management

Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later；In case of even The continuous rainy day, timely draining；Full exposure is cultivated, and promotes fringe bar lignifying；Within after transplanting 15 days, starting fertilising, the 3-8 month is every half The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 15kg, stops executing quick-acting fertilizer after JIUYUE, executes become thoroughly decomposed organic the 11-12 month 1 time Fertilizer, every mu of 1500kg, buriing of ditching.When spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, with no seedling injury Wood stem, branch and blade are as the criterion；After adopting fringe, execute 1 quick-acting fertilizer, every mu of 20kg immediately.Weeding to accomplish " except early, except little, remove ", substantially without weeds on seedbed；After rain, fine day weeds with a hoe in time, keeps soil from packing together.

2 breed

2.1 cut fringe

Early September carries out cutting fringe, 1 year 1 time, and cuttings length is 3cm, retains 2 lateral buds, 2 opposite leaf sheets, base portion flat nick. Cut fringe female parent height 15-20cm.

2.2 material

Using 72 hole dishes, specification is: wide 27cm, long 54cm, deep 5.5cm.Mesh bag specification is: diameter 4cm, deep 6cm.Described base Matter can use any value material mixing in following ranges to constitute: peat 30-40%, furfural dregs 5-10%, perlite 20-30%, Fine sand 10-20%, straw 10-20%, Herba Agerati Conyzoidis ground product 3-7%, violet stem and leaf ground product 2-4%.

2.3 cuttages and management

Using booth overhead seedbed cuttage, seedbed width 168cm, bed surface floor file cloth, laterally puts 3 hole tray or 3 pallets hold net Bag；Bed surface, hole tray, mesh bag are in advance with 800 times of liquid disinfectants of potassium permanganate.Using weight ratio is the naphthalene acetic acid of 3-5:3-5:1:1: Yin Indolylbutyric acid: antisepsis peptide of wilted hay bacilli: chrysanthemum total flavone extracting solution 1000ppm, quickly dipped fringe bar base portion 2 seconds, every cave or each 1 fringe bar of mesh bag cuttage, the cuttage degree of depth is 2cm；Water permeable after cuttage immediately, after blade face is dried, spray many bacterium of 1000 times of liquid Spirit, then covers plastic sheeting, is inserted into Small plastic shed, sealing film surrounding with bamboo chip, and in making canopy, air humidity reaches more than 95%.

Set sunshade net at big ceiling, above seedbed, (at high 2m), set up two road sunshade nets, when Air Temperature in plastic sheeting When degree is more than 38 DEG C, below two road sunshade nets, carry out mist cooling.Such as fine day high temperature, from point at 10 in the morning to afternoon 4, Spray 1 time every half an hour.Every day checks soil humidity, can not overly moist, also can not overdrying, when the thin film reverse side full little water droplet of knot, In Small plastic shed, relative air humidity is more than 95%, need not keep the skin wet；When little water droplet is little, water supply in media is not enough, be close to Late or cloudy, open thin film, water permeable, immediately after by sealing in film cover, typically water 1 water every 7-10 days.

2.4 seedling exercising

After cuttage about 25 days, the fringe bar root of hair of about 90% can start seedling exercising.First open thin film two to ventilate 2 days, then exist Dusk or cloudy day all open；Can manage with full exposure after 2 days at Small plastic shed upper cover last layer sunshade net after opening； After cuttage 10 days, start spray foliage fertilizer for the first time, later every 7-10 days, water and spray 1 time afterwards.Foliage fertilizer can be with green fragrant Wei 1000 times of liquid, or 0.2% carbamide sprays or waters and execute.In conjunction with diseases prevention, with 1000 times of liquid Mancozeb sprayings.

3 commercial seedlings make

3.1 Plug seedling

By in tloig-cutlage to the hole tray in 72 holes, through 6 months take pains to foster, form Plug seedling.Seedling specification: the long 4cm of young sprout, Well developed root system, robust plant, without pest and disease damage, yield rate 65%.

3.2 mesh bag Seedlings

By in tloig-cutlage to mesh bag, through 6 months take pains to foster, form mesh bag Seedling.Seedling specification: the long 4cm of young sprout, root system is sent out Reaching, substrate does not dissipates, robust plant, without pest and disease damage, yield rate 65%.

3.3 color lump Seedlings

Plug seedling or mesh bag Seedling are moved in container, through 24 months take pains to foster, forms color lump Seedling.Containers size: bore 12cm, degree of depth 10cm.Seedling specification: height of seedling 30cm, hat width 25cm, one-level branch 4, one-level branching-point height 10cm, two grades Branch 8, second branch point height 15cm, well developed root system, crown neatly, robust plant, without pest and disease damage, hat is well-balanced, finished product Rate 80%.

3.4 spherical Seedlings

Color lump Seedling moved in container or is transplanted to big field, through three prunings of 36 months with take pains to foster, forming spherical Seedling. Containers size: bore 26cm, degree of depth 21cm.Seedling specification: height of seedling 50cm, is preced with width 50cm, one-level branch 4, one-level branching-point Highly 10cm；Second branch 8, second branch point height 15cm；Three grades of branches 16, three grades of branching-point height 20cm, root system Prosperity, crown neatly, robust plant, without pest and disease damage, hat is well-balanced, yield rate 80%.

3.5 short hedge Seedlings

Color lump Seedling moved in container or is transplanted to big field, through four prunings of 40 months with take pains to foster, forming short hedge Seedling. Containers size: bore 26cm, degree of depth 21cm.Seedling specification: height of seedling 60cm, is preced with width 40cm, one-level branch 4, one-level branching-point Highly 10cm；Second branch 8, second branch point height 15cm；Three grades of branches 16, three grades of branching-point height 20cm, level Four Branch 24, level Four branching-point height 25cm, well developed root system, crown neatly, robust plant, without pest and disease damage, hat is well-balanced, finished product Rate 90%.

Claims (6)

1. floral leaf Buxus sempervirens/common box cuttage breeding method, it is characterised in that comprise the following steps:

1) choosing suitable breeding spot and set up floral leaf Buxus sempervirens/common box female parent cutting orchard, container seedling transplants seeding row spacing for transplanting seeding row spacing For 25cm × 35cm, density is 4000-5000 plant/acre；

2) using 72 hole dish cuttages, keep substrate elasticity consistent, described substrate contains the following material of weight percentage: Peat 30-40%, furfural dregs 5-10%, perlite 20-30%, fine sand 10-20%, straw 10-20%, Herba Agerati Conyzoidis ground product 3-7%, purple Rowland stem and leaf ground product 2-4%；

3) suitable cutting time is early September, and cuttage in 1 year 1 time, cuttings length is 2-3cm, retains 2 lateral buds, 2 opposite leaves Sheet, base portion flat nick；

4) cuttage branch uses and urges root agent 500-600ppm dipping fringe bar base portion 2-3 second, described in urge root agent be 3-by weight ratio The naphthalene acetic acid of 5:3-5:1:1: heteroauxing: antisepsis peptide of wilted hay bacilli: the mixing of chrysanthemum total flavone extracting solution is constituted；

5) the cuttage degree of depth is 2-3cm, waters permeable after cuttage immediately, sprays the carbendazim of 600-700 times of liquid, arch after the airing of blade face Canopy is cultivated, and in canopy, air humidity reaches more than 90%, after cuttage 10 days, starts spray foliage fertilizer for the first time, later every 7-10 days, Watering and spray 1 time afterwards, foliage fertilizer can spray with green fragrant 900-1000 times of liquid of Wei, simultaneously with 900-1000 times of liquid Mancozeb spray Mist diseases prevention, after cuttage 25 days, the fringe bar root of hair of about 90% can start seedling exercising.

2. floral leaf Buxus sempervirens/common box cuttage breeding method as claimed in claim 1, it is characterised in that in step 1):

The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed, Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower；Pavement width 40cm, deeply 25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded；

March in early November to next year transplants, and transplanting seeding row spacing is 25cm × 35cm, and density is 5000 plants/acre, and fixed point is dug Cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant, be compacted in root with fine earth heap, in time during transplanting Irrigating and determine root water, bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth；

Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later；In case of even The continuous rainy day, timely draining；Full exposure is cultivated, and promotes fringe bar lignifying；Within after transplanting 15 days, starting fertilising, the 3-8 month is every half The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 15kg, stops executing quick-acting fertilizer after JIUYUE, executes become thoroughly decomposed organic the 11-12 month 1 time Fertilizer, every mu of 1500kg, buriing of ditching, when spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, with no seedling injury Wood stem, branch and blade are as the criterion；After adopting fringe, execute 1 quick-acting fertilizer, every mu of 20kg immediately.

3. floral leaf Buxus sempervirens/common box cuttage breeding method as claimed in claim 1, it is characterised in that step 2) described in substrate contain There is a following material of weight percentage: peat 32-35%, furfural dregs 6-8%, perlite 25-28%, fine sand 12-18%, straw 12-15%, Herba Agerati Conyzoidis ground product 4-5%, violet stem and leaf ground product 3%.

4. floral leaf Buxus sempervirens/common box cuttage breeding method as claimed in claim 1, it is characterised in that step 2) described in cuttage Substrate uses following methods to prepare:

1) pretreatment: red Ji, violet stem and leaf are dried, are pulverized, prepares red Ji ground product, violet stem and leaf ground product；

2) by the following material mix homogeneously of weight percentage: peat 30-40%, furfural dregs 5-10%, perlite 20-30%, thin Husky 10-20%, straw 10-20%, Herba Agerati Conyzoidis ground product 3-7%, violet stem and leaf ground product 2-4%.

5. floral leaf Buxus sempervirens/common box cuttage breeding method as claimed in claim 1, it is characterised in that the Flos Chrysanthemi described in step 4) Total flavones extracting solution uses following methods to prepare:

1) Flos Chrysanthemi pulverizing is broken into coarse powder, cross 30 mesh sieves；

2) seepage pressure effects: Flos Chrysanthemi coarse powder is loaded in percolator, after soaking 10 hours with 75% aquiferous ethanol, use 70% aquiferous ethanol Carrying out seepage pressure effects, if raw material weight is 1kg, the consumption of aquiferous ethanol is 16 liters, and flow velocity is 5mL/min, collects percolate；

3) flash concentration: by percolate bath temperature 80 DEG C, vacuum is 0.095 Mpa, and feed liquor flow velocity is 300mL min^-1 Under conditions of carry out vacuum film concentration, reclaim ethanol, obtain every mL medicinal liquid and convert into the concentrated solution containing 2.0g raw material；

4) macroporous resin adsorption purification: by the concentrated solution that obtains by macroporous adsorbent resin separation and concentration, successively with water, 30% contain Water-ethanol, 40% aquiferous ethanol, 50% aquiferous ethanol, 60% aquiferous ethanol, 70% aquiferous ethanol carry out gradient elution, finally with 70% Acetone eluting, respectively collect 3 times of column volumes each position eluent, collect merge 40% aquiferous ethanol, 50% aquiferous ethanol, 60% The eluent at each position of aquiferous ethanol, is concentrated into the 1/4 of original volume by the eluent of merging, i.e. obtains chrysanthemum total flavone and extracts Liquid.

6. floral leaf Buxus sempervirens/common box cuttage breeding method as claimed in claim 1, it is characterised in that in step 4): naphthalene acetic acid: Yin Indolylbutyric acid: antisepsis peptide of wilted hay bacilli: the weight ratio of chrysanthemum total flavone extracting solution is 4:4:1:1.