CN106069528A - Radix Syzygii Buxifolii cuttage breeding method - Google Patents
Radix Syzygii Buxifolii cuttage breeding method Download PDFInfo
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- CN106069528A CN106069528A CN201610501250.2A CN201610501250A CN106069528A CN 106069528 A CN106069528 A CN 106069528A CN 201610501250 A CN201610501250 A CN 201610501250A CN 106069528 A CN106069528 A CN 106069528A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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Abstract
Radix Syzygii Buxifolii cuttage breeding method, belongs to technical field of plant propagation.Comprise the following steps: 1) choose suitable breeding spot and set up Radix Syzygii Buxifolii female parent cutting orchard;2) substrate is installed with 72 hole dishes;3) suitable cutting time is early September or the middle ten days, cuttage in 1 year 1 time;4) cuttage branch uses and urges root agent to impregnate fringe bar;5) after cuttage 38 days, the fringe bar root of hair of about 95% can start seedling exercising.Above-mentioned Radix Syzygii Buxifolii cuttage breeding method, simple to operate, reproduction speed is fast, has captured Radix Syzygii Buxifolii cottage propagation and has taken root the problem of difficulty; yield rate is up to 95%, can be used for commercial scale metaplasia and produces, reproducible; for domestic reported first, fill up the blank of domestic correlative technology field.
Description
Technical field
The invention belongs to technical field of plant propagation, specially Radix Syzygii Buxifolii cuttage breeding method.
Background technology
Radix Syzygii Buxifolii Syzygium buxifolium, Myrtaceae, Syzygium, evergreen broadleaved shrub, twig has rib, and tree crown is ellipse
Circular, spherical, wooden tough and tensile, bark dark brown, bronzing, rough, branch is dense, multi-branched, and withe is crisp easily broken;Dan Ye,
To life, leaf is little, keratin, wide ellipse, ellipse, wide obovate, closely lanceolar, long 1.5-3cm, wide 1-2cm, base portion wedge
Shape, nose-circle is blunt, Quan Yuan, pinnate vein, and master pulse caves in, and lateral vein is tiny the most inconspicuous, many and close, arteries and veins away from 1-1.5mm, diagonal to
On, away from leaf margin 1-1.5mm, it being combined into limit arteries and veins, leaf tool short handle, be about 2mm, spring, sprouting young leaves was red, tool sight, summer
Leaf green, yellow green.Cyme top is raw or axil is raw, and long 2.0-4.5cm spends several, the long 2.5mm of alabastrum, and petal 4 separates,
Floral white, long 2mm, the long 1-2mm of bennet, calyx tube turbination, it is about 2mm, the shallow waveform of calyx tooth, sliver 4, short and blunt;Stamen is many
Number, long 2.5mm, style is isometric with stamen, the month at florescence 5-7.Berry, oval, diameter 6-10mm, atropurpureus during fruit maturation,
Inside there are 1, seed, really the phase 9-10 month.Radix Syzygii Buxifolii happiness light, the most resistance to shade;High temperature resistant, the most cold-resistant, short-term can restrain oneself low temperature extremely-13 DEG C,
Subzero low temperature will make it by serious cold damage slightly for a long time, therefore in the area such as northern Suzhou, Wan Bei, surazo arrives it
Before Insulation to be taked, avoid dry freeze;Happiness acid ground;Like deep and rich in organic matter soil, avoid and execute dense fertilizer, the speed of growth
Slower.Common Diseases has red middle pinta and pestalotiopsis funerea, it should be noted that preventing and treating.It is distributed in the ground of warmer climate on the south the middle and lower reach of Yangtze River
Territory, the ground such as Guizhou, Hubei, Hunan, Anhui, Zhejiang, Fujian, Taiwan, Jiangxi, Guangdong, Guangxi.Radix Syzygii Buxifolii Technique on Cuttage Propagation at present
The most immature, existing fringe bar rooting rate is only about 70%, and Liu Wei etc. (2009) thinks that seminal propagation is easier to than cuttage, numerous
Grow coefficient higher.But seed genetic background is complicated, carries out large-scale breeding with seed and be unfavorable for the concordance of product, constrain red
The specialized large-scale development process of nanmu.Calendar year 2001, Senhe Seed Industry Co., Ltd., Zhejiang Pror. introduces red from Nanchang City, Jiangxi Province
Nanmu, through safety, regionality, compatibility test, and use different plant urge root agent, variable concentrations, different substrates formula and
The test of cottage method, it is thus achieved that success.Use sheet plant, avenue system, group planting, as color lump, short hedge, spherical tree, plant in garden, public affairs
By garden, river, beach, be the good color lump of sight, short hedge, spherical plant, there is wide market prospect.
Summary of the invention
For the above-mentioned problems in the prior art, it is an object of the invention to design provides a kind of Radix Syzygii Buxifolii cottage propagation
The technical scheme of method, comprises and sets up cutting orchard, and it is simple to operate, reproduction speed is fast, and yield rate is up to 95%.
Described Radix Syzygii Buxifolii cuttage breeding method, it is characterised in that comprise the following steps:
1) choosing suitable breeding spot and set up Radix Syzygii Buxifolii female parent cutting orchard, it is 35cm × 35cm that container seedling transplants seeding row spacing, and density is
3500-4000 plant/acre;
2) installing substrate with 72 hole dishes, keep substrate elasticity consistent, described substrate contains the following thing of weight percentage
Matter: reed jasmine 20-30%, Vermiculitum 10-15%, Maifanitum 10-20%, plant ash 5-10%, straw 10-20%, Celastrus angulatus ground product 5-
10%, Pericarpium Granati ground product 4-8%;
3) suitable cutting time is early September or the middle ten days, and cuttage in 1 year 1 time cuts fringe from Radix Syzygii Buxifolii female parent branch, and clip is selected in then
The spring tip and the intersection of autumn growth last year, be cut into the cuttage branch of a length of 2-3cm, retain 2 lateral buds, 2 opposite leaf sheets, base portion
Flat nick;
4) cuttage branch uses and urges root agent 500-600ppm dipping fringe bar base portion 2-3 second, described in urge root agent be 2-by weight ratio
The naphthalene acetic acid of 4:2-4:1-3:1-3: heteroauxing: the basic element of cell division: the mixing of Fericarpium Citri Limoniae total flavones extracting solution is constituted;
5) using seedbed cuttage, the cuttage degree of depth is 2-3cm, waters permeable after cuttage immediately, sprays 900-1000 after the airing of blade face
The carbendazim of times liquid, move-in-mud robot, in canopy, air humidity reaches more than 95%, after cuttage 10 days, starts spray foliage fertilizer for the first time,
Later every 7-10 days, watering and spray 1 time afterwards, foliage fertilizer can spray with green fragrant 600-700 times of liquid of Wei, uses 700-800 simultaneously
The spraying diseases prevention of times liquid Mancozeb, after cuttage 38 days, the fringe bar root of hair of about 95% can start seedling exercising.
Described Radix Syzygii Buxifolii cuttage breeding method, it is characterised in that in step 1):
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed,
Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower;Pavement width 40cm, deeply
25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded;
Carrying out container seedling transplanting March in early November to next year, transplanting seeding row spacing is 35cm × 35cm, and density is 3500 plants/acre, fixed
Point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant, be compacted in root with fine earth heap during transplanting,
Irrigating in time and determine root water, bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth;
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later;In case of even
The continuous rainy day, timely draining;Full exposure is cultivated, and promotes fringe bar lignifying;Within after transplanting 15 days, starting fertilising, the 3-8 month is every half
The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 15kg, stops executing quick-acting fertilizer after JIUYUE, executes become thoroughly decomposed organic the 11-12 month 1 time
Fertilizer, every mu of 1500kg, buriing of ditching.When spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, with no seedling injury
Wood stem, branch and blade are as the criterion;After adopting fringe, execute 1 quick-acting fertilizer, every mu of 20kg immediately.
Described Radix Syzygii Buxifolii cuttage breeding method, it is characterised in that step 2) described substrate contains below weight percentage
Material: reed jasmine 22-25%, Vermiculitum 12-13%, Maifanitum 12-15%, plant ash 8-9%, straw 12-15%, Celastrus angulatus ground product 7-
8%, Pericarpium Granati ground product 6-7%.
Described Radix Syzygii Buxifolii cuttage breeding method, it is characterised in that step 2) described in cutting medium use following methods system
Standby:
1) pretreatment: Celastrus angulatus, Pericarpium Granati are separately dried, are pulverized, prepares Celastrus angulatus ground product, Pericarpium Granati ground product;
2) by the following material mix homogeneously of weight percentage: reed jasmine 20-30%, Vermiculitum 10-15%, Maifanitum 10-20%, grass
Wood ash 5-10%, straw 10-20%, Celastrus angulatus ground product 5-10%, Pericarpium Granati ground product 4-8%.
Described Radix Syzygii Buxifolii cuttage breeding method, it is characterised in that the Fericarpium Citri Limoniae total flavones extracting solution described in step 4) uses
Prepared by following methods:
1) Fericarpium Citri Limoniae drying and crushing is broken into coarse powder, cross 20 mesh sieves;
2) seepage pressure effects: Fericarpium Citri Limoniae coarse powder is loaded in percolator, after soaking 5 hours with 80% aquiferous ethanol, by 70% aqueous second
Alcohol carries out seepage pressure effects, if raw material weight is 1kg, the consumption of aquiferous ethanol is 5 liters, and flow velocity is 6mL/min, collects percolate;
3) flash concentration: by percolate bath temperature 80 DEG C, vacuum is 0.09 Mpa, and feed liquor flow velocity is 200mL min-1
Under conditions of carry out vacuum film concentration, reclaim ethanol, obtain every mL medicinal liquid and convert into the concentrated solution containing 2.0g raw material;
4) macroporous resin adsorption purification: by the concentrated solution that obtains by macroporous adsorbent resin separation and concentration, successively with water, 10% contain
Water-ethanol, 20% aquiferous ethanol, 30% aquiferous ethanol, 40% aquiferous ethanol, 50% aquiferous ethanol, 60% aquiferous ethanol carry out gradient and wash
De-, finally with 70% acetone eluting, collect each position eluent of 3 times of column volumes respectively, collect merging 20% aquiferous ethanol, 30%
Aquiferous ethanol, 40% aquiferous ethanol, the eluent at the 50% each position of aquiferous ethanol, the eluent of merging is Fericarpium Citri Limoniae total flavones and carries
Take liquid.
Described Radix Syzygii Buxifolii cuttage breeding method, it is characterised in that in step 4): naphthalene acetic acid: heteroauxing: the basic element of cell division:
Fericarpium Citri Limoniae total flavones extracting solution weight ratio is 3:3:2:2.
Celastrus angulatus (Celastrus angulatus), belongs to Celastraceae (Celastraceae) Celastrus (Celastrus
) perennial liana, it is distributed widely in China the Yellow River, the hills of the Yangtze river basin and mountain area.Root bark and the peel of stem of Celastrus angulatus are equal
Containing multiple strength insecticidal constituent, from root bark or seed, isolation identification goes out dozens of noval chemical compound the most, particularly from kind
Oil obtains 4 crystallizations, be i.e. Celastrus angulatus ester I 1, from root bark, obtain 5 pure natural products, i.e. Celastrus angulatus I V.Closely
Studying discovery over Nian, the active insecticidal components of Celastrus angulatus has anesthesia, refusing to eat and stomach toxicity, action of contace poison, and does not produce drug resistance
Property, do not kill the feature such as natural enemy, stable in physicochemical property.
Pericarpium Granati is the peel of Punicaceae plant Punica granatum L., and peel contains tannin 10.4%, wax 0.8%, resin fat 4.5%, mannitol
1.8%, phlegmatic temperament 0.6%, gallic acid 4.0%, malic acid, pectin and calcium oxalate 4.0%, natural gum 3.2%, inulin 1.0%, noncrystalline
Sugar 2.7%, not only nutrient enriches, and also has anthelmintic, parasite killing, antiviral effect.
Celastrus angulatus ground product in above-mentioned substrate, Pericarpium Granati ground product, defending insect and killing is effective, the raw material source of employing in
Natural, belong to botanical pesticide, nontoxic to people, animal, and do not develop immunity to drugs, use safety.
Straw in above-mentioned substrate is pulverized 2-4cm respectively by Semen arachidis hypogaeae bar, bean bar, and adjustment water content 55-70%, C/N compare 30-
32, it is piled into ridge, with covered rearing with plastic film, makes the abundant fermentation maturity of straw, respectively obtain become thoroughly decomposed Semen arachidis hypogaeae bar and the bean bar that becomes thoroughly decomposed, will
Becoming thoroughly decomposed Semen arachidis hypogaeae bar and the ratio of the bean bar 2:1 that becomes thoroughly decomposed is mixed to prepare, have establishing in large scale in China, once, resource is rich for annual results
Rich.
Plant ash in above-mentioned substrate, be plant (draft and xylophyta) burning after ashes, so being all plants
Contained mineral element, nearly all contains in plant ash, and what wherein content was most is potassium element, general containing potassium 6 12%, wherein
90% is above water solublity, exists with carbonate form;Next to that phosphorus, general containing 1.5 3%;Possibly together with calcium, magnesium, silicon, sulfur and
The micronutrient elements such as ferrum, manganese, copper, zinc, boron, molybdenum, it is a kind of wide material sources, with low cost, nutrient is complete, fertilizer efficiency is obvious
Inorganic farm manure.
Maifanitum in above-mentioned substrate, belongs to volcanic rocks, and its main mine material is got angry mountain rock.Maifanitum is considered as
The lava that 5000-7550 ejects at 10000 years front volcanoes is embedded in underground, the high humidity through volcano, hot produced acidic materials with
And pressure produced by diastrophism and formed, Maifanitum originates in scabland.Maifanitum is a kind of natural medicine Ore, contains
There are 18 kinds of trace such as potassium necessary to human body, sodium, calcium, magnesium, phosphorus macroelement and zinc, ferrum, selenium, copper, strontium, iodine, fluorine, metasilicic acid
Element.
The cutting medium reasonable raw material proportioning that the present invention uses, the Celastrus angulatus ground product of mixed companion, Pericarpium Granati ground product are not only
Micronutrient element enriches, and owing to being botanical pesticide, and defending insect and killing effective, it is not necessary to additionally spray pest control with insecticide
Agent, environmentally safe, the nutrient of integrated substrate enriches, and micronutrient element is complete, has cuttage shoot growth amount significantly
Facilitation.
The above-mentioned basic element of cell division urged in root agent, calls 6-BA, 6-benzyl aminoadenine, is from Semen Maydis or other plant
A kind of N6-isopentennyladenine being separated to, at the material of the class promotion cytokinesis that plant root produces, promotes multiple group
The differentiation knitted and growth, have synergism, regulation plant cell growth and the phytohormone of growth with plant growing.
Urge addition Fericarpium Citri Limoniae total flavones extracting solution in root agent above-mentioned, the rooting rate of cuttage branch can be remarkably promoted, and
Improve its disease resistance.Containing substantial amounts of flavone compound in Fericarpium Citri Limoniae total flavones extracting solution, natural flavone compounds, do not contain
Any chemosynthesis class material, it can promote microorganism, plant cellular material operating and accumulation and cell enzyme activity, and then improve
The metabolic capacity of beneficial microbe bacterium, increases the disease resistance of plant, promotes plant growing.
In the present invention, cutting orchard is set up and management, cutting time, cuttings length, cutting medium form and quality, urge root agent
Concentration and formula is different, yield rate is all had a significant impact by management after cuttage.
Described reed jasmine, Vermiculitum, Maifanitum, plant ash, straw, Celastrus angulatus ground product, Pericarpium Granati ground product, naphthalene acetic acid,
Heteroauxing, the basic element of cell division, Fericarpium Citri Limoniae total flavones extracting solution, green fragrant Wei, Mancozeb all can the most directly be buied.
Above-mentioned Radix Syzygii Buxifolii cuttage breeding method, simple to operate, reproduction speed is fast, has captured Radix Syzygii Buxifolii cottage propagation and has taken root the asking of difficulty
Topic, yield rate is up to 95%, can be used for commercial scale metaplasia and produces, reproducible, for domestic reported first, fills up domestic relevant
The blank of technical field.
The percentage composition related in present specification unless otherwise indicated, refers both to weight percentage.
Detailed description of the invention
In conjunction with embodiments of the invention, the invention will be further described.
1 cutting orchard is set up and management
1.1 ground, gardens
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed,
Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower;Pavement width 40cm, deeply
25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded.
1.2 transplant
March in early November to next year transplants.Graphic by seeding row spacing design configurations, transplanting seeding row spacing is 35cm × 35cm, close
Degree is 3500 plants/acre.Fixed point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant during transplanting, with thin
Mound is compacted in root, irrigates in time and determines root water, and bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth.
1.3 management
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later;In case of even
The continuous rainy day, timely draining;Full exposure is cultivated, and promotes fringe bar lignifying;Within after transplanting 15 days, starting fertilising, the 3-8 month is every half
The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 15kg, stops executing quick-acting fertilizer after JIUYUE, executes become thoroughly decomposed organic the 11-12 month 1 time
Fertilizer, every mu of 1500kg, buriing of ditching.When spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, with no seedling injury
Wood stem, branch and blade are as the criterion;After adopting fringe, execute 1 quick-acting fertilizer, every mu of 20kg immediately.Weeding to accomplish " except early, except little, remove
", substantially without weeds on seedbed;After rain, fine day weeds with a hoe in time, keeps soil from packing together.
2 breed
2.1 cut fringe
Early September or the middle ten days carry out cutting fringe, 1 year 1 time, and clip is selected in the spring tip and the intersection of autumn growth last year, cuttings length then
For 2.5cm, retain 2 lateral buds, 2 opposite leaf sheets, base portion flat nick.Ground plants female parent then, cuts fringe height 15-25cm.
2.2 material
72 hole dish specifications are: wide 27cm, long 54cm, deep 5.5cm.Mesh bag specification is: diameter 4cm, deep 6cm.Described substrate contains
There is a following material of weight percentage: reed jasmine 20-30%, Vermiculitum 10-15%, Maifanitum 10-20%, plant ash 5-10%, straw
10-20%, Celastrus angulatus ground product 5-10%, Pericarpium Granati ground product 4-8%.
2.3 cuttages and management
Using booth overhead seedbed cuttage, seedbed width 168cm, bed surface floor file cloth, laterally puts 3 hole tray or 3 pallets hold net
Bag;Bed surface, hole tray, mesh bag are in advance with 800 times of liquid disinfectants of potassium permanganate.Using weight ratio is the naphthalene acetic acid of 3:3:2:2: indole second
Acid: the basic element of cell division: Fericarpium Citri Limoniae total flavones extracting solution 1000ppm, quickly dipped fringe bar base portion 2 seconds, every cave or each mesh bag cuttage 1
Fringe bar, the cuttage degree of depth is 2cm;Water permeable after cuttage immediately, after the airing of blade face, spray the carbendazim of 1000 times of liquid, then cover
Plastic sheeting, is inserted into Small plastic shed, sealing film surrounding with bamboo chip, and in making canopy, air humidity reaches more than 95%.
Set sunshade net at big ceiling, above seedbed, (at high 2m), set up two road sunshade nets, when Air Temperature in plastic sheeting
When degree is more than 38 DEG C, below two road sunshade nets, carry out mist cooling.Such as fine day high temperature, from point at 10 in the morning to afternoon 4,
Spray 1 time every half an hour.Every day checks soil humidity, can not overly moist, also can not overdrying, when the thin film reverse side full little water droplet of knot,
In Small plastic shed, relative air humidity is more than 95%, need not keep the skin wet;When little water droplet is little, water supply in media is not enough, be close to
Late or cloudy, open thin film, water permeable, immediately after by sealing in film cover, typically water 1 water every 7-10 days.
2.4 seedling exercising
After cuttage about 38 days, the fringe bar root of hair of about 95% can start seedling exercising.First open thin film two to ventilate 2 days, then exist
Dusk or cloudy day all open;Can manage with full exposure after 2 days at Small plastic shed upper cover last layer sunshade net after opening;
After cuttage 10 days, start spray foliage fertilizer for the first time, later every 7-10 days, water and spray 1 time afterwards.Foliage fertilizer can be with green fragrant Wei
1000 times of liquid, or 0.2% carbamide sprays or waters and execute.In conjunction with diseases prevention, with 1000 times of liquid Mancozeb sprayings.
3 commercial seedlings make
3.1 Plug seedling
By in tloig-cutlage to the hole tray in 72 holes, through 4.5 months take pains to foster, form Plug seedling.Seedling specification: young sprout is long
5cm, well developed root system, robust plant, without pest and disease damage, yield rate 95%.
3.2 mesh bag Seedlings
By in tloig-cutlage to mesh bag, through 4.5 months take pains to foster, form mesh bag Seedling.Seedling specification: the long 5cm of young sprout, root system
Prosperity, substrate does not dissipates, robust plant, without pest and disease damage, yield rate 95%.
3.3 color lump Seedlings
Plug seedling or mesh bag Seedling are moved in container, through the once prunings of 6.5 months with take pains to foster, form color lump Seedling.Container
Specification: bore 12cm, degree of depth 10cm.Seedling specification: height of seedling 30cm, is preced with width 25cm, one-level branch more than 5, one-level branching-point
Highly 10cm, yield rate 95%.
3.4 short hedge Seedlings
Color lump Seedling moved in container or is transplanted to big field, through the second prunings of 12 months with take pains to foster, forming short hedge Seedling.
Containers size: bore 26cm, degree of depth 21cm.Seedling specification: height of seedling 60cm, is preced with width 40cm, more than one-level branch 5 (containing), one
Level branching-point height 10cm;Second branch more than 10, one-level branching-point height 30cm, hat is well-balanced, yield rate 95%.
3.5 spherical Seedlings
Use ground to plant mode, by color lump transplantation of seedlings to big field, through 24 months prune and take pains to foster for three times, formed spherical
Seedling.Seedling specification: height of seedling 90cm, is preced with width 90cm, more than one-level branch 5 (containing), one-level branching-point height 10cm;Second branch
More than 10, one-level branching-point height 30cm;Three grades of branches more than 20, three grades of branching-point height 60cm, hat is well-balanced, finished product
Rate 95%.
Claims (6)
1. Radix Syzygii Buxifolii cuttage breeding method, it is characterised in that comprise the following steps:
1) choosing suitable breeding spot and set up Radix Syzygii Buxifolii female parent cutting orchard, it is 35cm × 35cm that container seedling transplants seeding row spacing, and density is
3500-4000 plant/acre;
2) installing substrate with 72 hole dishes, keep substrate elasticity consistent, described substrate contains the following thing of weight percentage
Matter: reed jasmine 20-30%, Vermiculitum 10-15%, Maifanitum 10-20%, plant ash 5-10%, straw 10-20%, Celastrus angulatus ground product 5-
10%, Pericarpium Granati ground product 4-8%;
3) suitable cutting time is early September or the middle ten days, and cuttage in 1 year 1 time cuts fringe from Radix Syzygii Buxifolii female parent branch, and clip is selected in then
The spring tip and the intersection of autumn growth last year, be cut into the cuttage branch of a length of 2-3cm, retain 2 lateral buds, 2 opposite leaf sheets, base portion
Flat nick;
4) cuttage branch uses and urges root agent 500-600ppm dipping fringe bar base portion 2-3 second, described in urge root agent be 2-by weight ratio
The naphthalene acetic acid of 4:2-4:1-3:1-3: heteroauxing: the basic element of cell division: the mixing of Fericarpium Citri Limoniae total flavones extracting solution is constituted;
5) using seedbed cuttage, the cuttage degree of depth is 2-3cm, waters permeable after cuttage immediately, sprays 900-1000 after the airing of blade face
The carbendazim of times liquid, move-in-mud robot, in canopy, air humidity reaches more than 95%, after cuttage 10 days, starts spray foliage fertilizer for the first time,
Later every 7-10 days, watering and spray 1 time afterwards, foliage fertilizer can spray with green fragrant 600-700 times of liquid of Wei, uses 700-800 simultaneously
The spraying diseases prevention of times liquid Mancozeb, after cuttage 38 days, the fringe bar root of hair of about 95% can start seedling exercising.
2. Radix Syzygii Buxifolii cuttage breeding method as claimed in claim 1, it is characterised in that in step 1):
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed,
Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower;Pavement width 40cm, deeply
25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded;
Carrying out container seedling transplanting March in early November to next year, transplanting seeding row spacing is 35cm × 35cm, and density is 3500 plants/acre, fixed
Point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant, be compacted in root with fine earth heap during transplanting,
Irrigating in time and determine root water, bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth;
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later;In case of even
The continuous rainy day, timely draining;Full exposure is cultivated, and promotes fringe bar lignifying;Within after transplanting 15 days, starting fertilising, the 3-8 month is every half
The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 15kg, stops executing quick-acting fertilizer after JIUYUE, executes become thoroughly decomposed organic the 11-12 month 1 time
Fertilizer, every mu of 1500kg, buriing of ditching;
When spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, be as the criterion with no seedling injury wood stem, branch and blade;
After adopting fringe, execute 1 quick-acting fertilizer, every mu of 20kg immediately.
3. Radix Syzygii Buxifolii cuttage breeding method as claimed in claim 1, it is characterised in that step 2) described substrate contains weight percent
The following material of content: reed jasmine 22-25%, Vermiculitum 12-13%, Maifanitum 12-15%, plant ash 8-9%, straw 12-15%, bitter skin
Rattan ground product 7-8%, Pericarpium Granati ground product 6-7%.
4. Radix Syzygii Buxifolii cuttage breeding method as claimed in claim 1, it is characterised in that step 2) described in cutting medium use with
Prepared by lower section method:
1) pretreatment: Celastrus angulatus, Pericarpium Granati are separately dried, are pulverized, prepares Celastrus angulatus ground product, Pericarpium Granati ground product;
2) by the following material mix homogeneously of weight percentage: reed jasmine 20-30%, Vermiculitum 10-15%, Maifanitum 10-20%, grass
Wood ash 5-10%, straw 10-20%, Celastrus angulatus ground product 5-10%, Pericarpium Granati ground product 4-8%.
5. Radix Syzygii Buxifolii cuttage breeding method as claimed in claim 1, it is characterised in that the Fericarpium Citri Limoniae total flavones described in step 4)
Extracting solution uses following methods to prepare:
1) Fericarpium Citri Limoniae drying and crushing is broken into coarse powder, cross 20 mesh sieves;
2) seepage pressure effects: Fericarpium Citri Limoniae coarse powder is loaded in percolator, after soaking 5 hours with 80% aquiferous ethanol, by 70% aqueous second
Alcohol carries out seepage pressure effects, if raw material weight is 1kg, the consumption of aquiferous ethanol is 5 liters, and flow velocity is 6mL/min, collects percolate;
3) flash concentration: by percolate bath temperature 80 DEG C, vacuum is 0.09 Mpa, and feed liquor flow velocity is 200mL min-1
Under conditions of carry out vacuum film concentration, reclaim ethanol, obtain every mL medicinal liquid and convert into the concentrated solution containing 2.0g raw material;
4) macroporous resin adsorption purification: by the concentrated solution that obtains by macroporous adsorbent resin separation and concentration, successively with water, 10% contain
Water-ethanol, 20% aquiferous ethanol, 30% aquiferous ethanol, 40% aquiferous ethanol, 50% aquiferous ethanol, 60% aquiferous ethanol carry out gradient and wash
De-, finally with 70% acetone eluting, collect each position eluent of 3 times of column volumes respectively, collect merging 20% aquiferous ethanol, 30%
Aquiferous ethanol, 40% aquiferous ethanol, the eluent at the 50% each position of aquiferous ethanol, the eluent of merging is Fericarpium Citri Limoniae total flavones and carries
Take liquid.
6. Radix Syzygii Buxifolii cuttage breeding method as claimed in claim 1, it is characterised in that in step 4): naphthalene acetic acid: heteroauxing: thin
Born of the same parents' mitogen: Fericarpium Citri Limoniae total flavones extracting solution weight ratio is 3:3:2:2.
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