CN105993563A - Mount tolmie dendrobenthamia japonica cutting propagation method - Google Patents
Mount tolmie dendrobenthamia japonica cutting propagation method Download PDFInfo
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a mount tolmie dendrobenthamia japonica cutting propagation method, and belongs to the technical field of plant propagation. The method comprises the following steps that 1, a proper cultivation place is selected for building a mount tolmie dendrobenthamia japonica female parent cutting orchard; 2, a 72-hole disc is adopted for cutting; 3, the suitable cutting time is the first ten-day period of September, and cutting is performed one time every year; 4, cutting branches are steeped by a root growing agent; 5, about 65% of scions are rooted 60 days after cutting is performed, and seedling hardening can be started. The mount tolmie dendrobenthamia japonica cutting propagation method is easy to operate, easy to operate and rapid in propagation speed, the rate of finished products reaches up to 90%, a gap of the related technical field is filled for reporting for the first time in China, the method can be used for large-scale production, the repeatability is good, and the good market prospect is achieved.
Description
Technical field
The invention belongs to technical field of plant propagation, specially mountain Tommy Japan Dendronenthamia japonica var.chinensis cuttage breeding method.
Background technology
Mountain Tommy Japan Dendronenthamia japonica var.chinensis Cornus kousa ' Satomi ', Cornaceae, wood belongs to, broad leaved and deciduous broad leaved dungarunga, and high 4.6-6.1m is preced with width 4.6-6.1m, bark polychrome, sepia, brown, reddish brown, Lycoperdon polymorphum Vitt.Dan Ye, to life, avette to heart, pinnate vein, master pulse lateral vein all sink, bend forward, and back side vein is the most protruding；Spring light green color, summer is green, gradually transfers peony, purple autumn to, leaf back slightly burnt hair.Spend little, both sexes, pink, red, dense, seem tree crown surface and one layer of color；The raw umbel in top, descends to give birth to the avette bract of 4 pieces of tips slightly point；Stamen 4, flower pesticide Long Circle；Floral disc cushion；Ovary is the next, and Room 2, there is ovule 1 every room；Style short cylindrical shape, stigma head；The month at florescence 5-6.Drupe, pellet shapes, edible, there is 2, seed.The mountain Tommy Japan Dendronenthamia japonica var.chinensis speed of growth is medium；Happiness acidity, subacidity, neutrality and alkalescence, moistening, fertile soil, well-drained environment；Happiness light, under full exposure or part illumination, growing way is best, and leaf color is the most ornamental；Such as long-time arid, it may occur that red middle pinta and pestalotiopsis funerea；Anthrax is had stronger resistance.
Mountain Tommy Japan Dendronenthamia japonica var.chinensis originates in China, Japan, Korea, can make specimen tree, foundation planting, mixed border, tree of shading.Japan's Dendronenthamia japonica var.chinensis is made briefly introduction as world-renowned ornamental trees by money spaces again etc. (2009), and the phyllary of its cultivar ' Japan beauty ' (Satomi, i.e. mountain Tommy) is red or pink, but does not report its Lycopersicum esculentum technology.2007, Senhe Seed Industry Co., Ltd., Zhejiang Pror. introduces ' mountain Tommy ' Japan Dendronenthamia japonica var.chinensis from Japan, through safety, regionality, compatibility test, and different plant is used to urge root agent, variable concentrations, different substrates formula and the test of cottage method, it is thus achieved that success.Subject range: Shaanxi, Henan, Hebei, Shandong, Anhui, Jiangsu, Zhejiang, Jiangxi, Hunan, Hubei, Sichuan, Guizhou, Yunnan.Isolated planting, sheet are planted, and as landscape tree, plant by garden, park, river, beach, are excellent Colored-leaf Plants in autumn.
Summary of the invention
For the above-mentioned problems in the prior art, it is an object of the invention to design provides the technical scheme of a kind of mountain Tommy Japan Dendronenthamia japonica var.chinensis cuttage breeding method, comprises and sets up cutting orchard, and it is simple to operate, reproduction speed is fast, and yield rate is up to 90%.
Described mountain Tommy Japan Dendronenthamia japonica var.chinensis cuttage breeding method, it is characterised in that comprise the following steps:
1) choosing suitable breeding spot and set up mountain Tommy Japan Dendronenthamia japonica var.chinensis female parent cutting orchard, it is 35cm × 35cm that container seedling transplants seeding row spacing, and density is 3500-4000 plant/acre；
2) 72 hole dish cuttages are used, keeping substrate elasticity consistent, described substrate contains the following material of weight percentage: peat 30-40%, distiller grains 5-15%, fine sand 10-20%, yellow soil 10-20%, straw 15-25%, Herba Agerati Conyzoidis ground product 4-6%, violet stem and leaf ground product 2-3%；
3) suitable cutting time is early September, and cuttage in 1 year 1 time, cuttings length is 4-5cm, retains 2 lateral buds, 2 opposite leaf sheets, base portion flat nick；
4) cuttage branch uses and urges root agent 500-600ppm dipping fringe bar base portion 2-3 second, described in urge root agent be the naphthalene acetic acid of 3-5:1-3:2-4:1 by weight ratio: ABT1 root-inducing powder: antisepsis peptide of wilted hay bacilli: the mixing of chrysanthemum total flavone extracting solution is constituted；
5) the cuttage degree of depth is 2-3cm, waters permeable after cuttage immediately, sprays the carbendazim of 600-700 times of liquid after the airing of blade face, move-in-mud robot, in canopy, air humidity reaches more than 90%, after cuttage 10 days, starts spray foliage fertilizer for the first time, later every 7-10 days, watering and spray 1 time afterwards, foliage fertilizer can spray with green fragrant 900-1000 times of liquid of Wei, simultaneously with 900-1000 times of liquid Mancozeb spraying diseases prevention, after cuttage 60 days, the fringe bar root of hair of about 65% can start seedling exercising.
Described mountain Tommy Japan Dendronenthamia japonica var.chinensis cuttage breeding method, it is characterised in that step 1)
Selecting that the soil texture is loose, fertile, subacidity, neutral make cutting orchard to subalkaline soil, turn over, ditch, make bed, open zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower；Pavement width 40cm, deep 25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded；
March in early November to next year transplants, transplanting seeding row spacing is 35cm × 35cm, density is 3500 plants/acre, fixed point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant during transplanting, it is compacted in root with fine earth heap, irrigating in time and determine root water, bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth；
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later；Such as continuous rainy day, timely draining；Full exposure is cultivated, and promotes fringe bar lignifying；Within after transplanting 15 days, start fertilising, the 3-8 month spreads fertilizer over the fields 1 carbamide or compound fertilizer every two weeks, each every mu of 15kg, stops after JIUYUE executing quick-acting fertilizer, executes 1 fertilizer become thoroughly decomposed the 11-12 month, every mu of 1500kg, trench digging buriing, when spreading fertilizer over the fields carbamide or compound fertilizer, is carried out in conjunction with weeding with a hoe, execute in the ranks, be as the criterion with no seedling injury wood stem, branch and blade；After adopting fringe, execute 1 quick-acting fertilizer, every mu of 20kg immediately.
Described mountain Tommy Japan Dendronenthamia japonica var.chinensis cuttage breeding method, it is characterised in that step 2) described in substrate contain the following material of weight percentage: peat 32-35%, distiller grains 8-10%, fine sand 12-15%, yellow soil 12-15%, straw 18-20%, Herba Agerati Conyzoidis ground product 5%, violet stem and leaf ground product 3%.
Described mountain Tommy Japan Dendronenthamia japonica var.chinensis cuttage breeding method, it is characterised in that step 2) described in cutting medium use following methods prepare:
1) pretreatment: red Ji, violet stem and leaf are dried, are pulverized, prepares red Ji ground product, violet stem and leaf ground product；
2) by the following material mix homogeneously of weight percentage: peat 30-40%, distiller grains 5-15%, fine sand 10-20%, yellow soil 10-20%, straw 15-25%, Herba Agerati Conyzoidis ground product 4-6%, violet stem and leaf ground product 2-3%.
Described mountain Tommy Japan Dendronenthamia japonica var.chinensis cuttage breeding method, it is characterised in that the chrysanthemum total flavone extracting solution described in step 4) uses following methods to prepare:
1) Flos Chrysanthemi pulverizing is broken into coarse powder, cross 30 mesh sieves；
2) seepage pressure effects: being loaded by Flos Chrysanthemi coarse powder in percolator, after soaking 10 hours with 75% aquiferous ethanol, carry out seepage pressure effects with 70% aquiferous ethanol, if raw material weight is 1kg, the consumption of aquiferous ethanol is 16 liters, and flow velocity is 5mL/min, collects percolate；
3) flash concentration: by percolate bath temperature 80 DEG C, vacuum is 0.095 Mpa, and feed liquor flow velocity is 300mL min-1Under conditions of carry out vacuum film concentration, reclaim ethanol, obtain every mL medicinal liquid and convert into the concentrated solution containing 2.0g raw material；
4) macroporous resin adsorption purification: the concentrated solution obtained is passed through macroporous adsorbent resin separation and concentration, gradient elution is carried out successively with water, 30% aquiferous ethanol, 40% aquiferous ethanol, 50% aquiferous ethanol, 60% aquiferous ethanol, 70% aquiferous ethanol, finally with 70% acetone eluting, collect each position eluent of 3 times of column volumes respectively, collect and merge 40% aquiferous ethanol, 50% aquiferous ethanol, the eluent at the 60% each position of aquiferous ethanol, the eluent of merging is concentrated into the 1/4 of original volume, i.e. obtains chrysanthemum total flavone extracting solution.
Described mountain Tommy Japan Dendronenthamia japonica var.chinensis cuttage breeding method, it is characterised in that in step 4): naphthalene acetic acid: ABT1 root-inducing powder: antisepsis peptide of wilted hay bacilli: the weight ratio of chrysanthemum total flavone extracting solution is 4:2:3:1.
Herba Agerati Conyzoidis, wild feverfew, originate in american torrid zone.Leaf oval, fissure, to life, spend blueness, pink, lavender or white, dense cluster is raw；Achene is little.The dwarf varieties in common flower garden Herba Agerati Conyzoidis (A. houstonianum, i.e. Ageratum houstonianum Miller.) is planted as edgewise plant.Herb frowziness, medicinal, heat-clearing and toxic substances removing, detumescence hemostasis, also there is stronger insecticidal effect.Herba Agerati Conyzoidis ground product in above-mentioned substrate, has disinsection efficiency, is mixed and accompanies in substrate, and slow release, long-acting pesticidal, the raw material source of employing in nature, belongs to botanical pesticide, do not develops immunity to drugs, uses safety.
Violet, originates in Mediterranean, and the extensively cultivation of current south China area, for the general designation of the plant of each kind under Cruciferae Matthiola incana.Violet stem and leaf ground product in above-mentioned cutting medium, plays and helps root effect.
Distiller grains in above-mentioned substrate are the side-products of brewing industry, containing crude protein 27.4%, crude fat 25%, crude fibre 10.5%, nitrogen-free extract 32.1%, cinder powder 5%.Distiller grains are added substrate, makes up the defect that the yellow soil content of organic matter is not enough, improve crop yield.And hydrophobic substance is more, imbitition is little because of protein etc. for distiller grains, therefore aeration porosity is relatively big, is 45.5%；And coconut palm bran is because main component is the hydrophilic substances such as cellulose, produces imbitition, cause aeration porosity less, only 4.7%.The addition of distiller grains, can improve the porosity physical behavior of substrate.
Straw in above-mentioned substrate is pulverized 2-4cm respectively by Semen arachidis hypogaeae bar, bean bar, adjust water content 55-70%, C/N compares 30-32, it is piled into ridge, with covered rearing with plastic film, make the abundant fermentation maturity of straw, respectively obtain become thoroughly decomposed Semen arachidis hypogaeae bar and the bean bar that becomes thoroughly decomposed, the ratio of become thoroughly decomposed Semen arachidis hypogaeae bar and the bean bar 2:1 that becomes thoroughly decomposed is mixed to prepare, having establishing in large scale in China, results are once every year, aboundresources.
The cutting medium reasonable raw material proportioning that the present invention uses, environmentally safe, the nutrient of integrated substrate enriches, and micronutrient element is complete, and cuttage shoot growth amount is had significant facilitation.
The above-mentioned antisepsis peptide of wilted hay bacilli urged in root agent, the antibacterial peptide matters extracted from antibacterial, it it is fermentable natural product, active height, pollution are less, the feature such as not accumulation in the environment, can stop the homergy approach of pathogenic microorganism or kill pathogenic microorganism and reach the purpose of controlling disease.
Urge addition chrysanthemum total flavone extracting solution in root agent above-mentioned, the rooting rate of cuttage branch can be remarkably promoted, and improve its disease resistance.Containing substantial amounts of flavone compound in chrysanthemum total flavone extracting solution, natural flavone compounds, without any chemosynthesis class material, it can promote microorganism, plant cellular material operating and accumulation and cell enzyme activity, and then improve the metabolic capacity of beneficial microbe bacterium, increase the disease resistance of plant, promote plant growing.
In the present invention, cutting orchard is set up and is formed the concentration with quality, urging root agent with management, cutting time, cuttings length, cutting medium and formula is different, yield rate is all had a significant impact by management after cuttage.The each component of cutting medium, proportioning have synergistic function.Each component, proportioning in root agent is urged also to have synergistic function.
Described domestic peat, distiller grains, perlite, yellow soil, straw, Herba Agerati Conyzoidis ground product, violet stem and leaf ground product, ABT1 root-inducing powder, heteroauxing, antisepsis peptide of wilted hay bacilli, chrysanthemum total flavone extracting solution, green fragrant Wei, Mancozeb all can the most directly be buied.
Above-mentioned mountain Tommy Japan Dendronenthamia japonica var.chinensis cuttage breeding method, simple to operate, reproduction speed is fast, and yield rate is up to 90%, for domestic reported first, fills up the blank of correlative technology field, can be used for large-scale production, favorable repeatability, has good market prospect.
The percentage composition related in present specification unless otherwise indicated, refers both to weight percentage.
Detailed description of the invention
In conjunction with embodiments of the invention, the invention will be further described.
1 cutting orchard is set up and management
1.1 ground, gardens
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed, opens zanjon, seedbed bed surface width 120cm around field, and bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower；Pavement width 40cm, deep 25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded.
1.2 transplant
March in early November to next year transplants.Graphic by seeding row spacing design configurations, transplanting seeding row spacing is 35cm × 35cm, and density is 3500 plants/acre.Fixed point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant during transplanting, with fine earth heap in root compacting, irrigates in time and determines root water, and bed surface spreads one layer of Caulis et Folium Oryzae, with moisturizing with prevent weed growth.
1.3 management
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later；Such as continuous rainy day, timely draining；Full exposure is cultivated, and promotes fringe bar lignifying；Within after transplanting 15 days, starting fertilising, the 3-8 month spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 15kg every two weeks, stops executing quick-acting fertilizer, execute 1 fertilizer become thoroughly decomposed, every mu of 1500kg the 11-12 month after JIUYUE, buriing of ditching.When spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, be as the criterion with no seedling injury wood stem, branch and blade；After adopting fringe, execute 1 quick-acting fertilizer, every mu of 20kg immediately.Weeding to accomplish " except early, except little, except ", substantially without weeds on seedbed；After rain, fine day weeds with a hoe in time, keeps soil from packing together.
1.4 prevent and treat red middle pinta and pestalotiopsis funerea
Red middle pinta is that on leaf, mycete and plan pestalotia bacteria cause, and endangers blade, occurs that russet, brown scab, sick strong intersection have a bronzing crown line at blade tip, leaf margin, and there is pore in disease portion, i.e. pathogen.There is black point in pestalotiopsis funerea disease portion, more apparent, during high humidity, has the sorus of black.Prevention and controls: removing sick leaf, concentration is burnt；Control density, keep ventilation and penetrating light；Strengthen management, strengthen plant resistance against diseases；Their early stage can spray 20% dragon 1000-1500 times of liquid of gram bacterium or 50% makes hectogram 800-1000 times liquid or 500 times of liquid of 80% zineb, 1 times a week, continuous 3 times.
2 breed
2.1 cut fringe
Early September carries out cutting fringe, 1 year 1 time, and clip is selected in the spring tip and the intersection of autumn growth last year then, and cuttings length is 4cm, retains 2 lateral buds, 2 opposite leaf sheets, base portion flat nick.Cut fringe and retain maternal height 20cm.
2.2 material
72 hole dish specifications are: wide 27cm, long 54cm, deep 5.5cm.Mesh bag specification is: diameter 6cm, deep 9cm.Described substrate can use the material mixing of any value in following ranges to constitute: peat 30-40%, distiller grains 5-15%, fine sand 10-20%, yellow soil 10-20%, straw 15-25%, Herba Agerati Conyzoidis ground product 4-6%, violet stem and leaf ground product 2-3%.
2.3 cuttages and management
Using booth overhead seedbed cuttage, seedbed width 168cm, bed surface floor file cloth, laterally puts 3 hole tray or 3 pallets hold mesh bag；Bed surface, hole tray, mesh bag are in advance with 800 times of liquid disinfectants of potassium permanganate.Using weight ratio is the naphthalene acetic acid of 4:2:3:1: ABT1 root-inducing powder: antisepsis peptide of wilted hay bacilli: chrysanthemum total flavone extracting solution 500ppm, quickly dipped fringe bar base portion 2 seconds, every cave or 1 fringe bar of each mesh bag cuttage, the cuttage degree of depth is 2cm；Watering permeable after cuttage immediately, spray the carbendazim of 1000 times of liquid, then cover plastic sheeting, be inserted into Small plastic shed, sealing film surrounding with bamboo chip after blade face is dried, in making canopy, air humidity reaches more than 95%.
Set sunshade net at big ceiling, above seedbed, (at high 2m), set up two road sunshade nets, when air themperature is more than 38 DEG C in plastic sheeting, below two road sunshade nets, carry out mist cooling.Such as fine day high temperature, from point at 10 in the morning to afternoon 4, spray 1 time every half an hour.Every day checks soil humidity, can not overly moist, also can not overdrying, when the thin film reverse side full little water droplet of knot, in Small plastic shed, relative air humidity is more than 95%, need not keep the skin wet；When little water droplet is little, water supply in media is not enough, between the lights or cloudy day, open thin film, water permeable, immediately after by film cover seal, typically watered 1 water every 7-10 days.
2.4 seedling exercising
After cuttage about 60 days, the fringe bar root of hair of about 65% can start seedling exercising.First open thin film two to ventilate 2 days, the most between the lights or the cloudy day all opens；Can manage with full exposure after 2 days at Small plastic shed upper cover last layer sunshade net after opening；After cuttage 10 days, start spray foliage fertilizer for the first time, later every 7-10 days, water and spray 1 time afterwards.Foliage fertilizer can be with green fragrant 1000 times of liquid of Wei, or 0.2% carbamide sprays or waters and executes.In conjunction with diseases prevention, with 1000 times of liquid Mancozeb sprayings.
3 commercial seedlings make
3.1 Plug seedling
By in tloig-cutlage to 72 hole dishes, through 7 months take pains to foster, form Plug seedling.Seedling specification: the long 6cm of young sprout, well developed root system, robust plant, without pest and disease damage, yield rate 65%.
3.2 mesh bag Seedlings
By in tloig-cutlage to mesh bag, through 7 months take pains to foster, form mesh bag Seedling.Seedling specification: the long 6cm of young sprout, well developed root system, substrate does not dissipates, robust plant, without pest and disease damage, yield rate 65%.
3.3 container seedling
Plug seedling or mesh bag Seedling are moved in container, through 14 months take pains to foster, forms container seedling.Containers size: bore 12cm, degree of depth 10cm.Seedling specification: the container seedling of height of seedling 30cm, not branch, yield rate 85%.
3.4 go alone Seedling
By height of seedling 30cm, during branch container seedling does not moves into 2 gallon container or be transplanted to big field, through 24 months take pains to foster, form not branch goes alone Seedling.Containers size: bore 26cm, degree of depth 21cm.Seedling specification: height of seedling 120cm, yield rate 90%.
Claims (6)
1. mountain Tommy Japan Dendronenthamia japonica var.chinensis cuttage breeding method, it is characterised in that comprise the following steps:
1) choosing suitable breeding spot and set up mountain Tommy Japan Dendronenthamia japonica var.chinensis female parent cutting orchard, it is 35cm × 35cm that container seedling transplants seeding row spacing, and density is 3500-4000 plant/acre；
2) 72 hole dish cuttages are used, keeping substrate elasticity consistent, described substrate contains the following material of weight percentage: peat 30-40%, distiller grains 5-15%, fine sand 10-20%, yellow soil 10-20%, straw 15-25%, Herba Agerati Conyzoidis ground product 4-6%, violet stem and leaf ground product 2-3%；
3) suitable cutting time is early September, and cuttage in 1 year 1 time, cuttings length is 4-5cm, retains 2 lateral buds, 2 opposite leaf sheets, base portion flat nick；
4) cuttage branch uses and urges root agent 500-600ppm dipping fringe bar base portion 2-3 second, described in urge root agent be the naphthalene acetic acid of 3-5:1-3:2-4:1 by weight ratio: ABT1 root-inducing powder: antisepsis peptide of wilted hay bacilli: the mixing of chrysanthemum total flavone extracting solution is constituted；
5) the cuttage degree of depth is 2-3cm, waters permeable after cuttage immediately, sprays the carbendazim of 600-700 times of liquid after the airing of blade face, move-in-mud robot, in canopy, air humidity reaches more than 90%, after cuttage 10 days, starts spray foliage fertilizer for the first time, later every 7-10 days, watering and spray 1 time afterwards, foliage fertilizer can spray with green fragrant 900-1000 times of liquid of Wei, simultaneously with 900-1000 times of liquid Mancozeb spraying diseases prevention, after cuttage 60 days, the fringe bar root of hair of about 65% can start seedling exercising.
2. mountain Tommy Japan as claimed in claim 1 Dendronenthamia japonica var.chinensis cuttage breeding method, it is characterised in that step 1)
Selecting that the soil texture is loose, fertile, subacidity, neutral make cutting orchard to subalkaline soil, turn over, ditch, make bed, open zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower；Pavement width 40cm, deep 25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded；
March in early November to next year transplants, transplanting seeding row spacing is 35cm × 35cm, density is 3500 plants/acre, fixed point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant during transplanting, it is compacted in root with fine earth heap, irrigating in time and determine root water, bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth；
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later；Such as continuous rainy day, timely draining；Full exposure is cultivated, and promotes fringe bar lignifying；Within after transplanting 15 days, start fertilising, the 3-8 month spreads fertilizer over the fields 1 carbamide or compound fertilizer every two weeks, each every mu of 15kg, stops after JIUYUE executing quick-acting fertilizer, executes 1 fertilizer become thoroughly decomposed the 11-12 month, every mu of 1500kg, trench digging buriing, when spreading fertilizer over the fields carbamide or compound fertilizer, is carried out in conjunction with weeding with a hoe, execute in the ranks, be as the criterion with no seedling injury wood stem, branch and blade；After adopting fringe, execute 1 quick-acting fertilizer, every mu of 20kg immediately.
3. mountain Tommy Japan as claimed in claim 1 Dendronenthamia japonica var.chinensis cuttage breeding method, it is characterised in that step 2) described in substrate contain the following material of weight percentage: peat 32-35%, distiller grains 8-10%, fine sand 12-15%, yellow soil 12-15%, straw 18-20%, Herba Agerati Conyzoidis ground product 5%, violet stem and leaf ground product 3%.
4. mountain Tommy Japan as claimed in claim 1 Dendronenthamia japonica var.chinensis cuttage breeding method, it is characterised in that step 2) described in cutting medium use following methods to prepare:
1) pretreatment: red Ji, violet stem and leaf are dried, are pulverized, prepares red Ji ground product, violet stem and leaf ground product；
2) by the following material mix homogeneously of weight percentage: peat 30-40%, distiller grains 5-15%, fine sand 10-20%, yellow soil 10-20%, straw 15-25%, Herba Agerati Conyzoidis ground product 4-6%, violet stem and leaf ground product 2-3%.
5. mountain Tommy Japan as claimed in claim 1 Dendronenthamia japonica var.chinensis cuttage breeding method, it is characterised in that the chrysanthemum total flavone extracting solution described in step 4) uses following methods to prepare:
1) Flos Chrysanthemi pulverizing is broken into coarse powder, cross 30 mesh sieves；
2) seepage pressure effects: being loaded by Flos Chrysanthemi coarse powder in percolator, after soaking 10 hours with 75% aquiferous ethanol, carry out seepage pressure effects with 70% aquiferous ethanol, if raw material weight is 1kg, the consumption of aquiferous ethanol is 16 liters, and flow velocity is 5mL/min, collects percolate；
3) flash concentration: by percolate bath temperature 80 DEG C, vacuum is 0.095 Mpa, and feed liquor flow velocity is 300mL min-1Under conditions of carry out vacuum film concentration, reclaim ethanol, obtain every mL medicinal liquid and convert into the concentrated solution containing 2.0g raw material；
4) macroporous resin adsorption purification: the concentrated solution obtained is passed through macroporous adsorbent resin separation and concentration, gradient elution is carried out successively with water, 30% aquiferous ethanol, 40% aquiferous ethanol, 50% aquiferous ethanol, 60% aquiferous ethanol, 70% aquiferous ethanol, finally with 70% acetone eluting, collect each position eluent of 3 times of column volumes respectively, collect and merge 40% aquiferous ethanol, 50% aquiferous ethanol, the eluent at the 60% each position of aquiferous ethanol, the eluent of merging is concentrated into the 1/4 of original volume, i.e. obtains chrysanthemum total flavone extracting solution.
6. mountain Tommy Japan as claimed in claim 1 Dendronenthamia japonica var.chinensis cuttage breeding method, it is characterised in that in step 4): naphthalene acetic acid: ABT1 root-inducing powder: antisepsis peptide of wilted hay bacilli: the weight ratio of chrysanthemum total flavone extracting solution is 4:2:3:1.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107251749A (en) * | 2017-07-12 | 2017-10-17 | 连云港西诺花卉种业有限公司 | A kind of small beautiful colored cuttage breeding method |
| CN108307825A (en) * | 2018-02-12 | 2018-07-24 | 广西壮族自治区农业科学院花卉研究所 | The method of Dendrobenthamia cuttage and quick-propagation |
| CN110506518A (en) * | 2019-09-09 | 2019-11-29 | 江苏农林职业技术学院 | A kind of cutting propagation rooting method of Dendronenthamia japonica var.chinensis |
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| CN107251749A (en) * | 2017-07-12 | 2017-10-17 | 连云港西诺花卉种业有限公司 | A kind of small beautiful colored cuttage breeding method |
| CN108307825A (en) * | 2018-02-12 | 2018-07-24 | 广西壮族自治区农业科学院花卉研究所 | The method of Dendrobenthamia cuttage and quick-propagation |
| CN110506518A (en) * | 2019-09-09 | 2019-11-29 | 江苏农林职业技术学院 | A kind of cutting propagation rooting method of Dendronenthamia japonica var.chinensis |
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