CN106134712A - The Snow Queen Quest oak leaf silk ball cuttage breeding method - Google Patents

The Snow Queen Quest oak leaf silk ball cuttage breeding method Download PDF

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Publication number
CN106134712A
CN106134712A CN201610501263.XA CN201610501263A CN106134712A CN 106134712 A CN106134712 A CN 106134712A CN 201610501263 A CN201610501263 A CN 201610501263A CN 106134712 A CN106134712 A CN 106134712A
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China
Prior art keywords
cuttage
root
quest
breeding method
silk ball
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CN201610501263.XA
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Inventor
郑勇平
陈岗
顾慧
吴光兄
余琼芳
罗红霞
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Zhejiang Senhe Seed Co Ltd
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Zhejiang Senhe Seed Co Ltd
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Priority to CN201610501263.XA priority Critical patent/CN106134712A/en
Publication of CN106134712A publication Critical patent/CN106134712A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for

Abstract

The Snow Queen Quest oak leaf silk ball cuttage breeding method, belongs to technical field of plant propagation.Comprise the following steps: 1) choose suitable breeding spot foundation fragrance snowball pod female parent cutting orchard;2) substrate is installed with 72 hole dishes;3) suitable cutting time is late September, cuttage in 1 year 1 time: 4) cuttage branch uses and urges root agent to impregnate;5) 72 hole dishes are used to carry out cuttage, 1 fringe bar of every cave cuttage;6) after cuttage 60 days, the fringe bar root of hair of about 60% can start seedling exercising.Above-mentioned The Snow Queen Quest oak leaf silk ball cuttage breeding method, adapts to the weather soil environment of China, takes root all right, and yield rate is up to 90%, and simple to operate, reproduction speed is fast;The method strong adaptability, can be used for commercial scale metaplasia and produces, and favorable repeatability, for domestic reported first, fills up the blank of domestic correlative technology field.

Description

The Snow Queen Quest oak leaf silk ball cuttage breeding method
Technical field
The invention belongs to technical field of plant propagation, specially The Snow Queen Quest oak leaf silk ball cuttage breeding method.
Background technology
The Snow Queen Quest oak leaf silk ball Hydrangea quercifolia ' Snow Queen ', Hydrangeaceae, hydrangea, fallen leaves Broadleaf shrubs, plant height 1.5-2.5m, is preced with width 1.0-2.0m.Dan Ye, to life, palmate blade, drastic crack, with blade ten split-phase of oriental white oak Seemingly;To autumn, can show, at bottle-green blade, the color change that it is surprising, from orange, redness to peony, pass layer by layer Enter, be very beautiful.Panicle, long 15-30cm, footpath 15-25cm, floral white, gradually take off and become pink or pansy, flower May phase.Happiness light, adapts to full exposure environment;Like acid ground moist, well-drained.Owing to blooming on old branch, so after spending (6-July) should prune immediately, beneficially the bud of tissue regeneration promoting the coming year.' The Snow Queen Quest ' oak leaf silk ball originates in southeastern US, and 2007 Year is introduced from Japan by Senhe Seed Industry Co., Ltd., Zhejiang Pror., through safety, regionality, compatibility test, and uses not Root agent, variable concentrations, different substrates formula and the test of cottage method is urged, it is thus achieved that success with plant.Subject range: Anhui, river Soviet Union, Zhejiang, Jiangxi, Hunan, Hubei, Sichuan, Guizhou, Yunnan.Isolated planting, sheet are planted, as hedge and specimen tree, lawn isolated planting, public affairs Both sides, road is afforested, and plants in garden, park, is excellent sight leaf and sight flower new varieties.Consult existing publication and document, still Without ' The Snow Queen Quest ' oak leaf silk ball related data.
Summary of the invention
For the above-mentioned problems in the prior art, it is an object of the invention to design provides a kind of The Snow Queen Quest oak leaf to embroider The technical scheme of ball cuttage breeding method, comprises and sets up cutting orchard, and it is simple to operate, reproduction speed is fast, and yield rate is up to 90%.
Described The Snow Queen Quest oak leaf silk ball cuttage breeding method, it is characterised in that comprise the following steps:
1) choose suitable breeding spot set up fragrance snowball pod female parent cutting orchard, cutting orchard transplant seeding row spacing be 40cm × 40cm, density is 2500-2800 plant/acre;
2) install substrate with 72 hole dishes, keep substrate elasticity consistent, first 24 hours of cuttage with 700-900 times of potassium permanganate Solution is to substrate disinfection, and described substrate contains the following material of volume ratio: peat 40-50%, coconut palm bran 20-30%, fine sand 5-10%, Maifanitum 8-15%, straw 10-20%, plant ash 5-10%, and by every cubic metre of cutting medium adds Bulbus Lycoridis Radiatae bulb powder Mince 200-300g, and ginkgo leaf powder minces 500-700g;
3) suitable cutting time is late September, and cuttage in 1 year 1 time plants maternal semi-lignified then from The Snow Queen Quest oak leaf silk ball Branch cuts fringe, is cut into the cuttage branch of a length of 4-5cm, retains 2 lateral buds, 2 opposite leaf sheets, and respectively cuts off half, base Portion's flat nick:
4) cuttage branch uses and urges root agent 900-1100ppm dipping fringe bar base portion 2-3 second, described in urge root agent be 4-by weight ratio The double gill GGR root-inducing powder of No. 6 of 6:1-3:1-3:0.5-1.5: naphthalene acetic acid: ABT1 root-inducing powder: Folium Ginkgo total flavones extracting solution Mixing is constituted;
5) using 72 hole dishes to carry out cuttage, 1 fringe bar of every cave cuttage, the cuttage degree of depth is 2-3cm, waters permeable after cuttage immediately, Spraying the carbendazim of 900-1000 times of liquid after the airing of blade face, move-in-mud robot, in canopy, air humidity reaches more than 95%, at booth Top sets sunshade net, sets up two road sunshade nets above seedbed, when in plastic sheeting, air themperature is more than 38 DEG C, hides in two roads Carry out mist cooling below screened postive, water 1 water every 5-8 days;Such as fine day high temperature, from point at 10 in the morning to afternoon 4, Mei Geban Hour spraying 1 time;
6) after cuttage 12 days, starting spray foliage fertilizer for the first time, later every 6-8 days, water and spray 1 time afterwards, foliage fertilizer can be used Green fragrant 900-1000 times of liquid of Wei sprays, simultaneously with 900-1000 times of liquid Mancozeb spraying diseases prevention;After cuttage 60 days, about 60% Fringe bar root of hair can start seedling exercising.
Described The Snow Queen Quest oak leaf silk ball cuttage breeding method, it is characterised in that in step 1):
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed, Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower;Pavement width 40cm, deeply 25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded;
March in early November to next year transplants, and transplanting seeding row spacing is 40cm × 40cm, and density is 2700 plants/acre, and fixed point is dug Cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant, be compacted in root with fine earth heap, in time during transplanting Irrigating and determine root water, bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth;
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later;In case of even The continuous rainy day, timely draining;Full exposure is cultivated, and promotes fringe bar lignifying;Within after transplanting 15 days, starting fertilising, the 4-9 month is every half The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 10-12kg, stop executing quick-acting fertilizer after October, executes the 11-12 month 1 time and becomes thoroughly decomposed Fertilizer, every mu of 1800-2000kg, buriing of ditching.When spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, It is as the criterion with no seedling injury wood stem, branch and blade;After adopting fringe, execute 1 quick-acting fertilizer, every mu of 10-12kg immediately.
Described The Snow Queen Quest oak leaf silk ball cuttage breeding method, it is characterised in that step 2) in:
The dry density 0.2-0.35 of described substrate, total porosity 85-90%, macroporsity 25-33%, aperture porosity 57-59%, Maximum specific retention 5.0-5.4, pH value 5.7-6.0, EC value 0.5-0.6, full nitrogen 1.2-1.4%, full phosphorus 490-500mg/kg, full potassium 6300-6400mg/kg, calcium 2600-2700mg/kg, magnesium 2400-2500mg/kg, copper 5-6mg/kg, zinc 50-55mg/kg, boron 5.0-5.5mg/kg, ferrum 4600-4700mg/kg, manganese 110-120mg/kg, molybdenum 1.6-2.0mg/kg, aluminum 2600-2700mg/kg, Lead 7-8mg/kg, sodium 3100-3200mg/kg, nickel 15-16mg/kg, sulfur 2400-2500mg/kg.
Described The Snow Queen Quest oak leaf silk ball cuttage breeding method, it is characterised in that step 2) in: peat 43-46%, coconut palm bran 22-27%, fine sand 6-8%, Maifanitum 10-12%, straw 12-15%, plant ash 6-8%, and add by every cubic metre of cutting medium Bulbus Lycoridis Radiatae bulb ground product 250-280g, ginkgo leaf powder minces 550-600g.
Described The Snow Queen Quest oak leaf silk ball cuttage breeding method, it is characterised in that step 2) described in cutting medium use Prepared by following methods:
1) pretreatment: Bulbus Lycoridis Radiatae bulb, Folium Ginkgo are separately dried, are pulverized, prepared Bulbus Lycoridis Radiatae bulb ground product, ginkgo leaf powder mince;
2) the following material of percent by volume is mixed: peat 40-50%, coconut palm bran 20-30%, fine sand 5-10%, Maifanitum 8-15%, Straw 10-20%, plant ash 5-10%, and by every cubic metre of cutting medium adds Bulbus Lycoridis Radiatae bulb ground product 200-300g, Semen Ginkgo Leaf ground product 500-700g.
Described The Snow Queen Quest oak leaf silk ball cuttage breeding method, it is characterised in that the Folium Ginkgo total flavones described in step 4) Extracting solution uses following methods to prepare:
1) Folium Ginkgo pulverizing is broken into coarse powder, cross 20 mesh sieves;
2) seepage pressure effects: Folium Ginkgo coarse powder is loaded in percolator, after soaking 8 hours with 70% aquiferous ethanol, by 75% aqueous second Alcohol carries out seepage pressure effects, if raw material weight is 1kg, the consumption of aquiferous ethanol is 8 liters, and flow velocity is 5mL/min, collects percolate;
3) flash concentration: by percolate bath temperature 70 DEG C, vacuum is 0.09 Mpa, and feed liquor flow velocity is 180mL min-1 Under conditions of carry out vacuum film concentration, reclaim ethanol, obtain every mL medicinal liquid and convert into the concentrated solution containing 1.0g raw material;
4) macroporous resin adsorption purification: by the concentrated solution that obtains by macroporous adsorbent resin separation and concentration, successively with water, 10% contain Water-ethanol, 25% aquiferous ethanol, 35% aquiferous ethanol, 45% aquiferous ethanol, 55% aquiferous ethanol, 70% aquiferous ethanol carry out gradient and wash De-, finally with 70% acetone eluting, collect each position eluent of 3 times of column volumes respectively, collection merging, 25% aquiferous ethanol, 35% Aquiferous ethanol, 45% aquiferous ethanol and the eluent at each position of 55% aquiferous ethanol, the eluent that will merge, obtain ginkgo biloba leaf total yellow Ketone extracting solution.
Described The Snow Queen Quest oak leaf silk ball cuttage breeding method, it is characterised in that in step 4): No. 6 double gill GGR are taken root Powder: naphthalene acetic acid: ABT1 root-inducing powder: the weight ratio of Folium Ginkgo total flavones extracting solution is that the mixing of 5:2:2:1 is constituted.
Bulbus Lycoridis Radiatae (Lycoris radiata) belongs to Monocotyledonae Bulbus Lycoridis Radiatae (Amaryllidaceae), lycoris plants Be used as medicinal plants very early to cure the disease and cure the wound, record according to Compendium of Material Medica etc.: Bulbus Lycoridis Radiatae not only have removing toxic substances, eliminate the phlegm, profit Urine, the multiple efficacies such as emetic, it may also be used for laryngopharynx swelling and pain, carbuncle sore tumefacting virus, scrofula, edema etc..Lycorine is a kind of iloquinoline derivative Alkaloid, for the derivant of pyridine in pyrroles's a pair of horses going side by side coffee.Research in recent years proves, lycorine have antiinflammatory, antiviral, malaria, The protection multiple effect such as cardiovascular and inducing apoptosis of tumour cell.About the pharmacological action of medicinal ingredient in lycoris plants with And the extraction and purification research of galantamine there are reports, but have no about the report for cutting medium.
Bulbus Lycoridis Radiatae bulb ground product in above-mentioned substrate, defending insect and killing is effective, and the raw material source of employing, in nature, belongs to and plants Physical property insecticide, nontoxic to people, animal, and do not develop immunity to drugs, use safety.
Straw in above-mentioned substrate is pulverized 2-4cm respectively by Semen arachidis hypogaeae bar, bean bar, and adjustment water content 55-70%, C/N compare 30- 32, it is piled into ridge, with covered rearing with plastic film, makes the abundant fermentation maturity of straw, respectively obtain become thoroughly decomposed Semen arachidis hypogaeae bar and the bean bar that becomes thoroughly decomposed, will Becoming thoroughly decomposed Semen arachidis hypogaeae bar and the ratio of the bean bar 2:1 that becomes thoroughly decomposed is mixed to prepare, have establishing in large scale in China, once, resource is rich for annual results Rich.
Plant ash in above-mentioned substrate, be plant (draft and xylophyta) burning after ashes, so being all plants Contained mineral element, nearly all contains in plant ash, and what wherein content was most is potassium element, general containing potassium 6 12%, wherein 90% is above water solublity, exists with carbonate form;Next to that phosphorus, general containing 1.5 3%;Possibly together with calcium, magnesium, silicon, sulfur and The micronutrient elements such as ferrum, manganese, copper, zinc, boron, molybdenum, it is a kind of wide material sources, with low cost, nutrient is complete, fertilizer efficiency is obvious Inorganic farm manure.
Maifanitum in above-mentioned substrate, belongs to volcanic rocks, and its main mine material is got angry mountain rock.Maifanitum is considered as The lava that 5000-7550 ejects at 10000 years front volcanoes is embedded in underground, the high humidity through volcano, hot produced acidic materials with And pressure produced by diastrophism and formed, Maifanitum originates in scabland.Maifanitum is a kind of natural medicine Ore, contains There are 18 kinds of trace such as potassium necessary to human body, sodium, calcium, magnesium, phosphorus macroelement and zinc, ferrum, selenium, copper, strontium, iodine, fluorine, metasilicic acid Element.
Containing substantial amounts of flavone compound in Folium Ginkgo, natural flavone compounds, without any chemosynthesis class thing Matter, it can promote microorganism, plant cellular material operating and accumulation and cell enzyme activity, and then improve the generation of beneficial microbe bacterium Thank to ability, increase the disease resistance of plant, promote plant growing.Ginkgo leaf powder in above-mentioned substrate minces, and can increase the anti-of plant Characteristic of disease, promotes plant growing.Urge addition Folium Ginkgo total flavones extracting solution in root agent above-mentioned, the life of cuttage branch can be remarkably promoted Root speed, and improve its disease resistance.
The cutting medium reasonable raw material proportioning that the present invention uses, has significant facilitation to cuttage shoot growth amount, also Having the effect of defending insect and killing, can guarantee that again micronutrient element is complete, its simple and convenient processing method, production cost is low.
In the present invention, cutting orchard is set up and management, cutting time, cuttings length, cutting medium form and quality, urge root agent Concentration and formula is different, yield rate is all had a significant impact by management after cuttage.
Described peat, coconut palm bran, fine sand, Maifanitum, straw, plant ash, Bulbus Lycoridis Radiatae bulb ground product, ginkgo leaf powder mince, No. 6 double gill GGR root-inducing powders, naphthalene acetic acid, ABT1 root-inducing powder, Folium Ginkgo total flavones extracting solution, green fragrant Wei, Mancozebs The most directly to buy.
Above-mentioned The Snow Queen Quest oak leaf silk ball cuttage breeding method, adapts to the weather soil environment of China, takes root all right, Yield rate is up to 90%, and simple to operate, reproduction speed is fast;The method strong adaptability, can be used for commercial scale metaplasia and produces, can weigh Renaturation is good, for domestic reported first, fills up the blank of domestic correlative technology field.
The percentage composition related in present specification unless otherwise indicated, refers both to weight percentage.
Detailed description of the invention
In conjunction with embodiments of the invention, the invention will be further described.
1 cutting orchard
1.1 ground, gardens
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed, Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower;Pavement width 40cm, deeply 25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded.
1.2 transplant
March in early November to next year transplants.Graphic by seeding row spacing design configurations, transplanting seeding row spacing is 40cm × 40cm, close Degree is 2700 plants/acre.Fixed point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant during transplanting, with thin Mound is compacted in root, irrigates in time and determines root water, and bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth.
1.3 management
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later;In case of even The continuous rainy day, timely draining;Full exposure is cultivated, and promotes fringe bar lignifying;Within after transplanting 15 days, starting fertilising, the 3-8 month is every half The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 15kg, stops executing quick-acting fertilizer after JIUYUE, executes become thoroughly decomposed organic the 11-12 month 1 time Fertilizer, every mu of 1500kg, buriing of ditching.When spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, with no seedling injury Wood stem, branch and blade are as the criterion;After adopting fringe, execute immediately 1 time quick-acting fat, every mu of 20kg.Weeding to accomplish " except early, except little, remove ", substantially without weeds on seedbed;After rain, fine day weeds with a hoe in time, keeps soil from packing together.
2 breed
2.1 cut fringe
Late September carries out cutting fringe, 1 year 1 time, and clip is selected in the spring tip and the intersection of autumn growth last year then, and cuttings length is 4cm, Retain 2 lateral buds, 2 opposite leaf sheets, and respectively cut off half, base portion flat nick.Ground plants female parent then, cuts fringe and retains female parent Highly 35cm;To life in 8 years and above no longer as female parent, height of seedling 120cm, the high hedge Seedling of hat width 60cm can be cultivated into;Or to 8 Year raw plant, takes stump measure in February, promotes maternal base portion to sprout rejuvenation, raising fringe bar quality.
2.2 material
Hole tray uses 72 hole dishes, and specification is: wide 27cm, long 54cm, deep 5.5cm.Mesh bag specification is: diameter 6cm, deep 8cm.Institute State substrate and contain the following material of volume ratio: peat 40-50%, coconut palm bran 20-30%, fine sand 5-10%, Maifanitum 8-15%, straw 10- 20%, plant ash 5-10%, and by adding Bulbus Lycoridis Radiatae bulb ground product 200-300g in every cubic metre of cutting medium, ginkgo leaf powder minces 500-700g。
2.3 cuttage
Using booth overhead seedbed cuttage, seedbed width 168cm, bed surface floor file cloth, laterally puts 3 hole tray or 3 pallets hold net Bag;Bed surface, hole tray, mesh bag and substrate are in advance with 800 times of liquid disinfectants of potassium permanganate.Employing weight ratio is 4-6:1-3:1-3:0.5- The double gill GGR root-inducing powder of No. 6 of 1.5: naphthalene acetic acid: ABT1 root-inducing powder: Folium Ginkgo total flavones extracting solution 500ppm, quickly dipped fringe bar Base portion 2 seconds, every cave or 1 fringe bar of each mesh bag cuttage, the cuttage degree of depth is 2cm;Water permeable after cuttage immediately, after the airing of blade face Spray the carbendazim of 1000 times of liquid, then cover plastic sheeting, be inserted into Small plastic shed, sealing film surrounding with bamboo chip, empty in making canopy Air humidity degree reaches more than 95%.Set sunshade net at big ceiling, above seedbed, (at high 2m), set up two road sunshade nets, when plastics are thin When air themperature is more than 38 DEG C in film, below two road sunshade nets, carry out mist cooling.Such as fine day high temperature, from point in the morning 10 To point in afternoon 4, spray 1 time every half an hour.Every day checks soil humidity, can not overly moist, also can not overdrying, when thin film reverse side tie Full little water droplet, in Small plastic shed, relative air humidity is more than 95%, need not keep the skin wet;When little water droplet is little, water supply in media is not enough Time, between the lights or cloudy day, open thin film, water permeable, immediately after by film cover seal, typically watered every 7-10 days 1 time Water.
2.4 seedling exercising
After cuttage about 60 days, the fringe bar root of hair of about 60% can start seedling exercising.First open thin film two to ventilate 2 days, then exist Dusk or cloudy day all open;Can manage with full exposure after 2 days at Small plastic shed upper cover last layer sunshade net after opening; After cuttage 10 days, start spray foliage fertilizer for the first time, later every 7-10 days, water and spray 1 time afterwards.Foliage fertilizer can be with green fragrant Wei 1000 times of liquid, or 0.2% carbamide sprays or waters and execute.In conjunction with diseases prevention, with 1000 times of liquid Mancozeb sprayings.
3 commercial seedlings make
3.1 Plug seedling
By in tloig-cutlage to the hole tray in 72 holes, through 4.5 months take pains to foster, form Plug seedling.Seedling specification: young sprout is long 6cm, well developed root system, robust plant, without pest and disease damage.The seedling time: from cuttage to seedling 4.5 months, yield rate 60%.
3.2 mesh bag Seedlings
By in tloig-cutlage to mesh bag, through 4.5 months take pains to foster, form mesh bag Seedling.Mesh bag specification: diameter 6cm, the degree of depth 8cm.Seedling specification: the long 6cm of young sprout, well developed root system, substrate does not dissipates, robust plant, without pest and disease damage.The seedling time: from cuttage to Seedling 4.5 months, yield rate 60%.
3.3 high hedge Seedlings
Plug seedling or mesh bag Seedling are moved in container or is transplanted to big field, through three prunings of 26 months with take pains to foster, shape Become high hedge Seedling.Containers size: bore 26cm, degree of depth 21cm.Seedling specification: height of seedling 120cm, is preced with width 60cm.The seedling time: from cave Dish Seedling or mesh bag Seedling move into container or are transplanted to land for growing field crops and start to emerging 26 months, yield rate 90%.

Claims (8)

1. The Snow Queen Quest oak leaf silk ball cuttage breeding method, it is characterised in that comprise the following steps:
1) choose suitable breeding spot set up fragrance snowball pod female parent cutting orchard, cutting orchard transplant seeding row spacing be 40cm × 40cm, density is 2500-2800 plant/acre;
2) install substrate with 72 hole dishes, keep substrate elasticity consistent, first 24 hours of cuttage with 700-900 times of potassium permanganate Solution is to substrate disinfection, and described substrate contains the following material of volume ratio: peat 40-50%, coconut palm bran 20-30%, fine sand 5-10%, Maifanitum 8-15%, straw 10-20%, plant ash 5-10%, and by every cubic metre of cutting medium adds Bulbus Lycoridis Radiatae bulb powder Mince 200-300g, and ginkgo leaf powder minces 500-700g;
3) suitable cutting time is late September, and cuttage in 1 year 1 time plants maternal semi-lignified then from The Snow Queen Quest oak leaf silk ball Branch cuts fringe, is cut into the cuttage branch of a length of 4-5cm, retains 2 lateral buds, 2 opposite leaf sheets, and respectively cuts off half, base Portion's flat nick:
4) cuttage branch uses and urges root agent 900-1100ppm dipping fringe bar base portion 2-3 second, described in urge root agent be 4-by weight ratio The double gill GGR root-inducing powder of No. 6 of 6:1-3:1-3:0.5-1.5: naphthalene acetic acid: ABT1 root-inducing powder: Folium Ginkgo total flavones extracting solution Mixing is constituted;
5) using 72 hole dishes to carry out cuttage, 1 fringe bar of every cave cuttage, the cuttage degree of depth is 2-3cm, waters permeable after cuttage immediately, Spraying the carbendazim of 900-1000 times of liquid after the airing of blade face, move-in-mud robot, in canopy, air humidity reaches more than 95%, at booth Top sets sunshade net, sets up two road sunshade nets above seedbed, when in plastic sheeting, air themperature is more than 38 DEG C, hides in two roads Carry out mist cooling below screened postive, water 1 water every 5-8 days;Such as fine day high temperature, from point at 10 in the morning to afternoon 4, Mei Geban Hour spraying 1 time;
6) after cuttage 12 days, starting spray foliage fertilizer for the first time, later every 6-8 days, water and spray 1 time afterwards, foliage fertilizer can be used Green fragrant 900-1000 times of liquid of Wei sprays, simultaneously with 900-1000 times of liquid Mancozeb spraying diseases prevention;After cuttage 60 days, about 60% Fringe bar root of hair can start seedling exercising.
2. The Snow Queen Quest oak leaf silk ball cuttage breeding method as claimed in claim 1, it is characterised in that in step 1):
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed, Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower;Pavement width 40cm, deeply 25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded;
March in early November to next year transplants, and transplanting seeding row spacing is 40cm × 40cm, and density is 2700 plants/acre, and fixed point is dug Cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant, be compacted in root with fine earth heap, in time during transplanting Irrigating and determine root water, bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth;
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later;In case of even The continuous rainy day, timely draining;Full exposure is cultivated, and promotes fringe bar lignifying;Within after transplanting 15 days, starting fertilising, the 4-9 month is every half The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 10-12kg, stop executing quick-acting fertilizer after October, executes the 11-12 month 1 time and becomes thoroughly decomposed Fertilizer, every mu of 1800-2000kg, buriing of ditching.
3. when spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, with no seedling injury wood stem, branch and blade be Accurate;After adopting fringe, execute 1 quick-acting fertilizer, every mu of 10-12kg immediately.
4. The Snow Queen Quest oak leaf silk ball cuttage breeding method as claimed in claim 1, it is characterised in that step 2) in:
The dry density 0.2-0.35 of described substrate, total porosity 85-90%, macroporsity 25-33%, aperture porosity 57-59%, Maximum specific retention 5.0-5.4, pH value 5.7-6.0, EC value 0.5-0.6, full nitrogen 1.2-1.4%, full phosphorus 490-500mg/kg, full potassium 6300-6400mg/kg, calcium 2600-2700mg/kg, magnesium 2400-2500mg/kg, copper 5-6mg/kg, zinc 50-55mg/kg, boron 5.0-5.5mg/kg, ferrum 4600-4700mg/kg, manganese 110-120mg/kg, molybdenum 1.6-2.0mg/kg, aluminum 2600-2700mg/kg, Lead 7-8mg/kg, sodium 3100-3200mg/kg, nickel 15-16mg/kg, sulfur 2400-2500mg/kg.
5. The Snow Queen Quest oak leaf silk ball cuttage breeding method as claimed in claim 1, it is characterised in that step 2) in: peat 43- 46%, coconut palm bran 22-27%, fine sand 6-8%, Maifanitum 10-12%, straw 12-15%, plant ash 6-8%, and by every cubic metre of cuttage base Adding Bulbus Lycoridis Radiatae bulb ground product 250-280g in matter, ginkgo leaf powder minces 550-600g.
6. The Snow Queen Quest oak leaf silk ball cuttage breeding method as claimed in claim 1, it is characterised in that step 2) described in skewer Inserting substrate uses following methods to prepare:
1) pretreatment: Bulbus Lycoridis Radiatae bulb, Folium Ginkgo are separately dried, are pulverized, prepared Bulbus Lycoridis Radiatae bulb ground product, ginkgo leaf powder mince;
2) the following material of percent by volume is mixed: peat 40-50%, coconut palm bran 20-30%, fine sand 5-10%, Maifanitum 8-15%, Straw 10-20%, plant ash 5-10%, and by every cubic metre of cutting medium adds Bulbus Lycoridis Radiatae bulb ground product 200-300g, Semen Ginkgo Leaf ground product 500-700g.
7. The Snow Queen Quest oak leaf silk ball cuttage breeding method as claimed in claim 1, it is characterised in that the silver described in step 4) Folium Pruni total flavones extracting solution uses following methods to prepare:
1) Folium Ginkgo pulverizing is broken into coarse powder, cross 20 mesh sieves;
2) seepage pressure effects: Folium Ginkgo coarse powder is loaded in percolator, after soaking 8 hours with 70% aquiferous ethanol, by 75% aqueous second Alcohol carries out seepage pressure effects, if raw material weight is 1kg, the consumption of aquiferous ethanol is 8 liters, and flow velocity is 5mL/min, collects percolate;
3) flash concentration: by percolate bath temperature 70 DEG C, vacuum is 0.09 Mpa, and feed liquor flow velocity is 180mL min-1 Under conditions of carry out vacuum film concentration, reclaim ethanol, obtain every mL medicinal liquid and convert into the concentrated solution containing 1.0g raw material;
4) macroporous resin adsorption purification: by the concentrated solution that obtains by macroporous adsorbent resin separation and concentration, successively with water, 10% contain Water-ethanol, 25% aquiferous ethanol, 35% aquiferous ethanol, 45% aquiferous ethanol, 55% aquiferous ethanol, 70% aquiferous ethanol carry out gradient and wash De-, finally with 70% acetone eluting, collect each position eluent of 3 times of column volumes respectively, collection merging, 25% aquiferous ethanol, 35% Aquiferous ethanol, 45% aquiferous ethanol and the eluent at each position of 55% aquiferous ethanol, the eluent that will merge, obtain ginkgo biloba leaf total yellow Ketone extracting solution.
8. The Snow Queen Quest oak leaf silk ball cuttage breeding method as claimed in claim 1, it is characterised in that in step 4): No. 6 double lucky That GGR root-inducing powder: naphthalene acetic acid: ABT1 root-inducing powder: the weight ratio of Folium Ginkgo total flavones extracting solution is the mixing structure of 5:2:2:1 Become.
CN201610501263.XA 2016-06-30 2016-06-30 The Snow Queen Quest oak leaf silk ball cuttage breeding method Pending CN106134712A (en)

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