CN106171877A - Big Ye Fusejila wood cuttage breeding method - Google Patents
Big Ye Fusejila wood cuttage breeding method Download PDFInfo
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- CN106171877A CN106171877A CN201610501270.XA CN201610501270A CN106171877A CN 106171877 A CN106171877 A CN 106171877A CN 201610501270 A CN201610501270 A CN 201610501270A CN 106171877 A CN106171877 A CN 106171877A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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Abstract
Big Ye Fusejila wood cuttage breeding method, belongs to technical field of plant propagation.Comprise the following steps: 1) choose suitable breeding spot and set up big Ye Fusejila wood female parent cutting orchard；2) substrate is installed with 72 hole dishes；3) suitable cutting time is early September, cuttage in 1 year 1 time；4) cuttage branch uses and urges root agent to impregnate；5) 72 hole dishes are used to carry out cuttage；6) after cuttage 40 days, the fringe bar root of hair of about 95% can start seedling exercising.Above-mentioned big Ye Fusejila wood cuttage breeding method, adapts to the weather soil environment of China, takes root all right, and yield rate is up to 95%, and simple to operate, reproduction speed is fast；The method strong adaptability, can be used for commercial scale metaplasia and produces, and favorable repeatability, for domestic reported first, fills up the blank of domestic correlative technology field.
Description
Technical field
The invention belongs to technical field of plant propagation, the biggest Ye Fusejila wood cuttage breeding method.
Background technology
Big Ye Fusejila wood Fothergilla major ' Mount Airy ', Hamamelidaceae, Fu Sejila wood belongs to, falls
Leaf broadleaf shrubs, plant height 1.5-2.5m, it is preced with width 1.0-1.8m, Dan Ye, alternate, pinnate vein, blade quality is coarse, aeruginous, deep
Green, inner side slightly turns white, and Autumn leaf colours transfers to orange red, red, of dazzling beauty.Grey colour band fragrance, hairbrush shape, the month at florescence 3-5.
Happiness full exposure or part illumination, growing way is medium, and happiness is moistening but requires that planting soil draining is good, medium to the demand of water, typically
Within one week, normally water once, especially in the first growth phase, need rule water to form the most wide root system, during high temperature
Water requirement increases.Winter in evening carries out pruning to promote New shoot growth before spring has set in.Foundation planting, hedge can be made, it is possible to make cut-flower.Great Ye
Fu Sejila wood originates in North America, within 2007, is introduced from Japan by Senhe Seed Industry Co., Ltd., Zhejiang Pror., through safety, region
Property, compatibility test, and use different plant to urge root agent, variable concentrations, different substrates formula and the test of cottage method, obtain
Obtain success.Subject range: Shaanxi, Henan, Hebei, Shandong, Anhui, Jiangsu, Jiangxi, Zhejiang, Hunan, Hubei, Sichuan, Guizhou,
Yunnan.Isolated planting, sheet are planted, and as specimen tree, lawn isolated planting, the greening of highway both sides, outdoor plants courtyard garden, coastal saline-alkali area
Also can plant.Planting by garden, park, river, beach, tool sight, is red autumnal leaves new varieties and beach greening in excellent autumn
Seeds.Consulting existing publication and document, there is no big Ye Fusejila wood related data, this field still belongs to blank.
Summary of the invention
For the above-mentioned problems in the prior art, it is an object of the invention to design provides a kind of big Ye Fusejila
The technical scheme of wood cuttage breeding method, comprises and sets up cutting orchard, and it is simple to operate, reproduction speed is fast, and yield rate is up to 95%.
Described big Ye Fusejila wood cuttage breeding method, it is characterised in that comprise the following steps:
1) choose suitable breeding spot and set up big Ye Fusejila wood female parent cutting orchard, cutting orchard transplant seeding row spacing be 40cm ×
40cm, density is 2500-2800 plant/acre；
2) install substrate with 72 hole dishes, keep substrate elasticity consistent, first 24 hours of cuttage with 700-900 times of potassium permanganate
Solution is to substrate disinfection, and described substrate contains the following material of volume ratio: peat 30-40%, Vermiculitum 20-30%, fine sand
5-10%, Maifanitum 5-10%, straw 10-20%, plant ash 5-15%, and by every cubic metre of cutting medium adds Bulbus Lycoridis Radiatae bulb powder
Mince 300-400g, and ginkgo leaf powder minces 200-300g；
3) suitable cutting time is early September, and cuttage in 1 year 1 time cuts fringe from big Ye Fusejila wood female parent branch, and clip is selected in
The spring tip and the intersection of autumn growth last year, be cut into the cuttage branch of a length of 4-5cm, retain 1 lateral bud, 1 blade then, and
Respectively cut off half, base portion flat nick；
4) cuttage branch uses and urges root agent 900-1100ppm dipping fringe bar base portion 2-3 second, described in urge root agent be 4-by weight ratio
The naphthalene acetic acid of 6:1-3:1-3:0.5-1.5: No. 6 double gill GGR root-inducing powders: ABT1 root-inducing powder: Folium Ginkgo total flavones extracting solution
Mixing is constituted；
5) using 72 hole dishes to carry out cuttage, 1 fringe bar of every cave cuttage, the cuttage degree of depth is 2-3cm, waters permeable after cuttage immediately,
Spraying the carbendazim of 700-800 times of liquid after the airing of blade face, move-in-mud robot, in canopy, air humidity reaches more than 95%, at big ceiling
If sunshade net, above seedbed, set up two road sunshade nets, when air themperature is more than 38 DEG C in plastic sheeting, in two road sunshades
Side off the net carried out mist cooling, watered 1 water every 7-10 days；Such as fine day high temperature, from point at 10 in the morning to afternoon 4, Mei Geban
Hour spraying 1 time；
6) after cuttage 12 days, starting spray foliage fertilizer for the first time, later every 7-10 days, water and spray 1 time afterwards, foliage fertilizer can be used
Green fragrant 900-1000 times of liquid of Wei sprays, simultaneously with 900-1000 times of liquid Mancozeb spraying diseases prevention；After cuttage 40 days, about 95%
Fringe bar root of hair can start seedling exercising.
Described big Ye Fusejila wood cuttage breeding method, it is characterised in that in step 1):
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed,
Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower；Pavement width 40cm, deeply
25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded；
Carrying out container seedling transplanting March in early November to next year, transplanting seeding row spacing is 35cm × 35cm, and density is 3500 plants/acre, fixed
Point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant, be compacted in root with fine earth heap during transplanting,
Irrigating in time and determine root water, bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth；
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later；In case of even
The continuous rainy day, timely draining；Full exposure is cultivated, and promotes fringe bar lignifying；Within after transplanting 15 days, starting fertilising, the 4-7 month is every half
The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 10-12kg, stop executing quick-acting fertilizer after August, executes the 11-12 month 1 time and becomes thoroughly decomposed
Fertilizer, every mu of 1500kg, buriing of ditching, when spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, with not
Hinder nursery stock stem, branch and blade to be as the criterion；After adopting fringe, execute 1 quick-acting fertilizer, every mu of 10-15kg immediately.
Described big Ye Fusejila wood cuttage breeding method, it is characterised in that step 2) in:
The dry density 0.2-0.35 of described substrate, total porosity 85-90%, macroporsity 25-33%, aperture porosity 57-59%,
Maximum specific retention 5.0-5.4, pH value 5.7-6.0, EC value 0.5-0.6, full nitrogen 1.2-1.4%, full phosphorus 490-500mg/kg, full potassium
6300-6400mg/kg, calcium 2600-2700mg/kg, magnesium 2400-2500mg/kg, copper 5-6mg/kg, zinc 50-55mg/kg, boron
5.0-5.5mg/kg, ferrum 4600-4700mg/kg, manganese 110-120mg/kg, molybdenum 1.6-2.0mg/kg, aluminum 2600-2700mg/kg,
Lead 7-8mg/kg, sodium 3100-3200mg/kg, nickel 15-16mg/kg, sulfur 2400-2500mg/kg.
Described big Ye Fusejila wood cuttage breeding method, it is characterised in that step 2) in:
Peat 35-38%, Vermiculitum 22-25%, fine sand 6-8%, Maifanitum 6-8%, straw 12-15%, plant ash 8-10%, and by often standing
Adding Bulbus Lycoridis Radiatae bulb ground product 350-380g in side's rice cutting medium, ginkgo leaf powder minces 220-250g.
Described big Ye Fusejila wood cuttage breeding method, it is characterised in that step 2) described in cutting medium use
Prepared by following methods:
1) pretreatment: Bulbus Lycoridis Radiatae bulb, Folium Ginkgo are separately dried, are pulverized, prepared Bulbus Lycoridis Radiatae bulb ground product, ginkgo leaf powder mince；
2) the following material of percent by volume is mixed: peat 30-40%, Vermiculitum 20-30%, fine sand 5-10%, Maifanitum 5-10%,
Straw 10-20%, plant ash 5-15%, and by every cubic metre of cutting medium adds Bulbus Lycoridis Radiatae bulb ground product 300-400g, Semen Ginkgo
Leaf ground product 200-300g.
Described big Ye Fusejila wood cuttage breeding method, it is characterised in that the Folium Ginkgo total flavones described in step 4)
Extracting solution uses following methods to prepare:
1) Folium Ginkgo pulverizing is broken into coarse powder, cross 20 mesh sieves；
2) seepage pressure effects: Folium Ginkgo coarse powder is loaded in percolator, after soaking 8 hours with 70% aquiferous ethanol, by 75% aqueous second
Alcohol carries out seepage pressure effects, if raw material weight is 1kg, the consumption of aquiferous ethanol is 8 liters, and flow velocity is 5mL/min, collects percolate；
3) flash concentration: by percolate bath temperature 70 DEG C, vacuum is 0.09 Mpa, and feed liquor flow velocity is 180mL min-1
Under conditions of carry out vacuum film concentration, reclaim ethanol, obtain every mL medicinal liquid and convert into the concentrated solution containing 1.0g raw material；
4) macroporous resin adsorption purification: by the concentrated solution that obtains by macroporous adsorbent resin separation and concentration, successively with water, 10% contain
Water-ethanol, 25% aquiferous ethanol, 35% aquiferous ethanol, 45% aquiferous ethanol, 55% aquiferous ethanol, 70% aquiferous ethanol carry out gradient and wash
De-, finally with 70% acetone eluting, collect each position eluent of 3 times of column volumes respectively, collection merging, 25% aquiferous ethanol, 35%
Aquiferous ethanol, 45% aquiferous ethanol and the eluent at each position of 55% aquiferous ethanol, the eluent that will merge, obtain ginkgo biloba leaf total yellow
Ketone extracting solution.
Described big Ye Fusejila wood cuttage breeding method, it is characterised in that in step 4): indolebutyric acid: naphthalene acetic acid:
ABT6 root-inducing powder: the weight ratio of Folium Ginkgo total flavones extracting solution is 5:2:2:1.
Bulbus Lycoridis Radiatae (Lycoris radiata) belongs to Monocotyledonae Bulbus Lycoridis Radiatae (Amaryllidaceae), lycoris plants
Be used as medicinal plants very early to cure the disease and cure the wound, record according to Compendium of Material Medica etc.: Bulbus Lycoridis Radiatae not only have removing toxic substances, eliminate the phlegm, profit
Urine, the multiple efficacies such as emetic, it may also be used for laryngopharynx swelling and pain, carbuncle sore tumefacting virus, scrofula, edema etc..Lycorine is a kind of iloquinoline derivative
Alkaloid, for the derivant of pyridine in pyrroles's a pair of horses going side by side coffee.Research in recent years proves, lycorine have antiinflammatory, antiviral, malaria,
The protection multiple effect such as cardiovascular and inducing apoptosis of tumour cell.About the pharmacological action of medicinal ingredient in lycoris plants with
And the extraction and purification research of galantamine there are reports, but have no about the report for cutting medium.
Bulbus Lycoridis Radiatae bulb ground product in above-mentioned substrate, defending insect and killing is effective, and the raw material source of employing, in nature, belongs to and plants
Physical property insecticide, nontoxic to people, animal, and do not develop immunity to drugs, use safety.
Straw in above-mentioned substrate is pulverized 2-4cm respectively by Semen arachidis hypogaeae bar, bean bar, and adjustment water content 55-70%, C/N compare 30-
32, it is piled into ridge, with covered rearing with plastic film, makes the abundant fermentation maturity of straw, respectively obtain become thoroughly decomposed Semen arachidis hypogaeae bar and the bean bar that becomes thoroughly decomposed, will
Becoming thoroughly decomposed Semen arachidis hypogaeae bar and the ratio of the bean bar 2:1 that becomes thoroughly decomposed is mixed to prepare, have establishing in large scale in China, once, resource is rich for annual results
Rich.
Plant ash in above-mentioned substrate, be plant (draft and xylophyta) burning after ashes, so being all plants
Contained mineral element, nearly all contains in plant ash, and what wherein content was most is potassium element, general containing potassium 6 12%, wherein
90% is above water solublity, exists with carbonate form；Next to that phosphorus, general containing 1.5 3%；Possibly together with calcium, magnesium, silicon, sulfur and
The micronutrient elements such as ferrum, manganese, copper, zinc, boron, molybdenum, it is a kind of wide material sources, with low cost, nutrient is complete, fertilizer efficiency is obvious
Inorganic farm manure.
Maifanitum in above-mentioned substrate, belongs to volcanic rocks, and its main mine material is got angry mountain rock.Maifanitum is considered as
The lava that 5000-7550 ejects at 10000 years front volcanoes is embedded in underground, the high humidity through volcano, hot produced acidic materials with
And pressure produced by diastrophism and formed, Maifanitum originates in scabland.Maifanitum is a kind of natural medicine Ore, contains
There are 18 kinds of trace such as potassium necessary to human body, sodium, calcium, magnesium, phosphorus macroelement and zinc, ferrum, selenium, copper, strontium, iodine, fluorine, metasilicic acid
Element.
Containing substantial amounts of flavone compound in Folium Ginkgo, natural flavone compounds, without any chemosynthesis class thing
Matter, it can promote microorganism, plant cellular material operating and accumulation and cell enzyme activity, and then improve the generation of beneficial microbe bacterium
Thank to ability, increase the disease resistance of plant, promote plant growing.Ginkgo leaf powder in above-mentioned substrate minces, and can increase the anti-of plant
Characteristic of disease, promotes plant growing.Urge addition Folium Ginkgo total flavones extracting solution in root agent above-mentioned, the life of cuttage branch can be remarkably promoted
Root speed, and improve its disease resistance.
The cutting medium reasonable raw material proportioning that the present invention uses, has significant facilitation to cuttage shoot growth amount, also
Having the effect of defending insect and killing, can guarantee that again micronutrient element is complete, its simple and convenient processing method, production cost is low.
In the present invention, cutting orchard is set up and management, cutting time, cuttings length, cutting medium form and quality, urge root agent
Concentration and formula is different, yield rate is all had a significant impact by management after cuttage.
Described peat, Vermiculitum, fine sand, Maifanitum, straw, plant ash, Bulbus Lycoridis Radiatae bulb ground product, ginkgo leaf powder mince,
Naphthalene acetic acid, No. 6 double gill GGR root-inducing powders, ABT1 root-inducing powder, Folium Ginkgo total flavones extracting solution, green fragrant Wei, Mancozebs
The most directly to buy.
Above-mentioned big Ye Fusejila wood cuttage breeding method, adapts to the weather soil environment of China, takes root all right,
Yield rate is up to 95%, and simple to operate, reproduction speed is fast；The method strong adaptability, can be used for commercial scale metaplasia and produces, can weigh
Renaturation is good, for domestic reported first, fills up the blank of domestic correlative technology field.
The percentage composition related in present specification unless otherwise indicated, refers both to weight percentage.
Detailed description of the invention
In conjunction with embodiments of the invention, the invention will be further described.
1 cutting orchard
1.1 ground, gardens
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed,
Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower；Pavement width 40cm, deeply
25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded.
1.2 transplant
March in early November to next year transplants.Graphic by seeding row spacing design configurations, transplanting seeding row spacing is 35cm × 35cm, close
Degree is 3500 plants/acre.Fixed point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant during transplanting, with thin
Mound is compacted in root, irrigates in time and determines root water, and bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth.
1.3 management
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later；In case of even
The continuous rainy day, timely draining；Full exposure is cultivated, and promotes fringe bar lignifying；Within after transplanting 15 days, starting fertilising, the 3-8 month is every half
The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 15kg, stops executing quick-acting fertilizer after JIUYUE, executes become thoroughly decomposed organic the 11-12 month 1 time
Fertilizer, every mu of 1500kg, buriing of ditching.When spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, with no seedling injury
Wood stem, branch and blade are as the criterion；After adopting fringe, execute immediately 1 time quick-acting fat, every mu of 20kg.Weeding to accomplish " except early, except little, remove
", substantially without weeds on seedbed；After rain, fine day weeds with a hoe in time, keeps soil from packing together.
2 breed
2.1 cut fringe
Early September carries out cutting fringe, 1 year 1 time, and clip is selected in the spring tip and the intersection of autumn growth last year then, and cuttings length is
3.5cm, retains 1 lateral bud, 1 blade, and respectively cuts off half, base portion flat nick.Retaining maternal height is 35cm.
2.2 material
Hole tray uses 72 hole dishes, and specification is: wide 27cm, long 54cm, deep 5.5cm.Mesh bag specification is: diameter 6cm, deep 8cm.Institute
State substrate and contain the following material of volume ratio: peat 30-40%, Vermiculitum 20-30%, fine sand 5-10%, Maifanitum 5-10%, straw 10-
20%, plant ash 5-15%, and by adding Bulbus Lycoridis Radiatae bulb ground product 300-400g in every cubic metre of cutting medium, ginkgo leaf powder minces
200-300g。
2.3 cuttages and management
Using booth overhead seedbed cuttage, seedbed width 168cm, bed surface floor file cloth, laterally puts 3 hole tray or 3 pallets hold net
Bag；Bed surface, hole tray, mesh bag and substrate are in advance with 800 times of liquid disinfectants of potassium permanganate.Using urges root agent by weight ratio described in weight ratio
Naphthalene acetic acid for 4-6:1-3:1-3:0.5-1.5: No. 6 double gill GGR root-inducing powders: ABT1 root-inducing powder: Folium Ginkgo total flavones carries
Taking liquid 1000ppm, quickly dipped fringe bar base portion 2 seconds, every cave or 1 fringe bar of each mesh bag cuttage, the cuttage degree of depth is 2cm；Stand after cuttage
I.e. water permeable, after the airing of blade face, spray the carbendazim of 1000 times of liquid, then cover plastic sheeting, be inserted into Small plastic shed with bamboo chip,
Sealing film surrounding, in making canopy, air humidity reaches more than 95%.
Set sunshade net at big ceiling, above seedbed, (at high 2m), set up two road sunshade nets, when Air Temperature in plastic sheeting
When degree is more than 38 DEG C, below two road sunshade nets, carry out mist cooling.Such as fine day high temperature, from point at 10 in the morning to afternoon 4,
Spray 1 time every half an hour.Every day checks soil humidity, can not overly moist, also can not overdrying, when the thin film reverse side full little water droplet of knot,
In Small plastic shed, relative air humidity is more than 95%, need not keep the skin wet；When little water droplet is little, water supply in media is not enough, be close to
Late or cloudy, open thin film, water permeable, immediately after by sealing in film cover, typically water 1 water every 7-10 days.
2.4 seedling exercising
After cuttage about 40 days, the fringe bar root of hair of about 95% can start seedling exercising.First open thin film two to ventilate 2 days, then exist
Dusk or cloudy day all open；Can manage with full exposure after 2 days at Small plastic shed upper cover last layer sunshade net after opening；
After cuttage 10 days, start spray foliage fertilizer for the first time, later every 7-10 days, water and spray 1 time afterwards.Foliage fertilizer can be with green fragrant Wei
1000 times of liquid, or 0.2% carbamide sprays or waters and execute.In conjunction with diseases prevention, with 1000 times of liquid Mancozeb sprayings.
3 commercial seedlings make
3.1 Plug seedling
By in tloig-cutlage to the hole tray in 72 holes, through 5 months take pains to foster, form Plug seedling.Seedling specification: the long 6cm of young sprout,
Well developed root system, robust plant, without pest and disease damage.The seedling time: from cuttage to seedling 5 months, yield rate 95%.
3.2 mesh bag Seedlings
By in tloig-cutlage to mesh bag, through 5 months take pains to foster, form mesh bag Seedling.Mesh bag specification: diameter 6cm, degree of depth 8cm.
Seedling specification: the long 6cm of young sprout, well developed root system, substrate does not dissipates, robust plant, without pest and disease damage.The seedling time: from cuttage to seedling 5
Individual month, yield rate 95%.
3.3 color lump Seedlings
Plug seedling or mesh bag Seedling are moved in container, through 8 months take pains to foster, forms color lump Seedling.Containers size: bore
12cm, degree of depth 10cm.Seedling specification: height of seedling 30cm.The seedling time: move into container from Plug seedling or mesh bag Seedling and start to emerging 8
Individual month, yield rate 90%.
3.4 short hedge Seedlings
Color lump Seedling moved in container or is transplanted to big field, through the second prunings of 10 months with take pains to foster, forming short hedge Seedling.
Containers size: bore 26cm, degree of depth 21cm.Seedling specification: height of seedling 60cm, is preced with width 40cm, and hat is well-balanced.The seedling time: from color
Block Seedling moves into container or is transplanted to land for growing field crops and starts to emerging 10 months, yield rate 90%.
3.5 high hedge Seedlings
Color lump Seedling moved in container or is transplanted to big field, through three prunings of 24 months with take pains to foster, forming high hedge Seedling.
Containers size: bore 26cm, degree of depth 21cm.Seedling specification: height of seedling 120cm, is preced with width 60cm, and hat is well-balanced.The seedling time: from color
Block Seedling moves into container or is transplanted to land for growing field crops and starts to emerging 24 months, yield rate 90%.
Claims (7)
1. big Ye Fusejila wood cuttage breeding method, it is characterised in that comprise the following steps:
1) choose suitable breeding spot and set up big Ye Fusejila wood female parent cutting orchard, cutting orchard transplant seeding row spacing be 40cm ×
40cm, density is 2500-2800 plant/acre；
2) install substrate with 72 hole dishes, keep substrate elasticity consistent, first 24 hours of cuttage with 700-900 times of potassium permanganate
Solution is to substrate disinfection, and described substrate contains the following material of volume ratio: peat 30-40%, Vermiculitum 20-30%, fine sand
5-10%, Maifanitum 5-10%, straw 10-20%, plant ash 5-15%, and by every cubic metre of cutting medium adds Bulbus Lycoridis Radiatae bulb powder
Mince 300-400g, and ginkgo leaf powder minces 200-300g；
3) suitable cutting time is early September, and cuttage in 1 year 1 time cuts fringe from big Ye Fusejila wood female parent branch, and clip is selected in
The spring tip and the intersection of autumn growth last year, be cut into the cuttage branch of a length of 4-5cm, retain 1 lateral bud, 1 blade then, and
Respectively cut off half, base portion flat nick；
4) cuttage branch uses and urges root agent 900-1100ppm dipping fringe bar base portion 2-3 second, described in urge root agent be 4-by weight ratio
The naphthalene acetic acid of 6:1-3:1-3:0.5-1.5: No. 6 double gill GGR root-inducing powders: ABT1 root-inducing powder: Folium Ginkgo total flavones extracting solution
Mixing is constituted；
5) using 72 hole dishes to carry out cuttage, 1 fringe bar of every cave cuttage, the cuttage degree of depth is 2-3cm, waters permeable after cuttage immediately,
Spraying the carbendazim of 700-800 times of liquid after the airing of blade face, move-in-mud robot, in canopy, air humidity reaches more than 95%, at big ceiling
If sunshade net, above seedbed, set up two road sunshade nets, when air themperature is more than 38 DEG C in plastic sheeting, in two road sunshades
Side off the net carried out mist cooling, watered 1 water every 7-10 days；Such as fine day high temperature, from point at 10 in the morning to afternoon 4, Mei Geban
Hour spraying 1 time；
6) after cuttage 12 days, starting spray foliage fertilizer for the first time, later every 7-10 days, water and spray 1 time afterwards, foliage fertilizer can be used
Green fragrant 900-1000 times of liquid of Wei sprays, simultaneously with 900-1000 times of liquid Mancozeb spraying diseases prevention；After cuttage 40 days, about 95%
Fringe bar root of hair can start seedling exercising.
2. big Ye Fusejila wood cuttage breeding method as claimed in claim 1, it is characterised in that in step 1):
The selection soil texture is loose, fertile, cutting orchard is made in subacidity, neutral extremely subalkaline soil, turns over, ditches, makees bed,
Opening zanjon, seedbed bed surface width 120cm around field, bed surface soil block is whole carefully, and middle slightly higher, both sides are slightly lower；Pavement width 40cm, deeply
25cm, low centre, pavement two is slightly higher, forms certain gradient, it is ensured that draining is unimpeded；
Carrying out container seedling transplanting March in early November to next year, transplanting seeding row spacing is 35cm × 35cm, and density is 3500 plants/acre, fixed
Point digs cave, wipes out long side root, keeps the degree of depth consistent with the degree of depth of original plant, be compacted in root with fine earth heap during transplanting,
Irrigating in time and determine root water, bed surface one layer of Caulis et Folium Oryzae of paving, with moisturizing with prevent weed growth；
Seedling-slowing stage waters in time, such as continuous sunny, within the 4th day after transplanting, need to water, water once every 10 days later；In case of even
The continuous rainy day, timely draining；Full exposure is cultivated, and promotes fringe bar lignifying；Within after transplanting 15 days, starting fertilising, the 4-7 month is every half
The moon spreads fertilizer over the fields 1 carbamide or compound fertilizer, each every mu of 10-12kg, stop executing quick-acting fertilizer after August, executes the 11-12 month 1 time and becomes thoroughly decomposed
Fertilizer, every mu of 1500kg, buriing of ditching, when spreading fertilizer over the fields carbamide or compound fertilizer, carry out in conjunction with weeding with a hoe, execute in the ranks, with not
Hinder nursery stock stem, branch and blade to be as the criterion；After adopting fringe, execute 1 quick-acting fertilizer, every mu of 10-15kg immediately.
3. big Ye Fusejila wood cuttage breeding method as claimed in claim 1, it is characterised in that step 2) in:
The dry density 0.2-0.35 of described substrate, total porosity 85-90%, macroporsity 25-33%, aperture porosity 57-59%,
Maximum specific retention 5.0-5.4, pH value 5.7-6.0, EC value 0.5-0.6, full nitrogen 1.2-1.4%, full phosphorus 490-500mg/kg, full potassium
6300-6400mg/kg, calcium 2600-2700mg/kg, magnesium 2400-2500mg/kg, copper 5-6mg/kg, zinc 50-55mg/kg, boron
5.0-5.5mg/kg, ferrum 4600-4700mg/kg, manganese 110-120mg/kg, molybdenum 1.6-2.0mg/kg, aluminum 2600-2700mg/kg,
Lead 7-8mg/kg, sodium 3100-3200mg/kg, nickel 15-16mg/kg, sulfur 2400-2500mg/kg.
4. big Ye Fusejila wood cuttage breeding method as claimed in claim 1, it is characterised in that step 2) in:
Peat 35-38%, Vermiculitum 22-25%, fine sand 6-8%, Maifanitum 6-8%, straw 12-15%, plant ash 8-10%, and by often standing
Adding Bulbus Lycoridis Radiatae bulb ground product 350-380g in side's rice cutting medium, ginkgo leaf powder minces 220-250g.
5. Ye Fusejila wood cuttage breeding method as claimed in claim 1 big, it is characterised in that step 2) described in skewer
Inserting substrate uses following methods to prepare:
1) pretreatment: Bulbus Lycoridis Radiatae bulb, Folium Ginkgo are separately dried, are pulverized, prepared Bulbus Lycoridis Radiatae bulb ground product, ginkgo leaf powder mince；
2) the following material of percent by volume is mixed: peat 30-40%, Vermiculitum 20-30%, fine sand 5-10%, Maifanitum 5-10%,
Straw 10-20%, plant ash 5-15%, and by every cubic metre of cutting medium adds Bulbus Lycoridis Radiatae bulb ground product 300-400g, Semen Ginkgo
Leaf ground product 200-300g.
6. big Ye Fusejila wood cuttage breeding method as claimed in claim 1, it is characterised in that the silver described in step 4)
Folium Pruni total flavones extracting solution uses following methods to prepare:
1) Folium Ginkgo pulverizing is broken into coarse powder, cross 20 mesh sieves；
2) seepage pressure effects: Folium Ginkgo coarse powder is loaded in percolator, after soaking 8 hours with 70% aquiferous ethanol, by 75% aqueous second
Alcohol carries out seepage pressure effects, if raw material weight is 1kg, the consumption of aquiferous ethanol is 8 liters, and flow velocity is 5mL/min, collects percolate；
3) flash concentration: by percolate bath temperature 70 DEG C, vacuum is 0.09 Mpa, and feed liquor flow velocity is 180mL min-1
Under conditions of carry out vacuum film concentration, reclaim ethanol, obtain every mL medicinal liquid and convert into the concentrated solution containing 1.0g raw material；
4) macroporous resin adsorption purification: by the concentrated solution that obtains by macroporous adsorbent resin separation and concentration, successively with water, 10% contain
Water-ethanol, 25% aquiferous ethanol, 35% aquiferous ethanol, 45% aquiferous ethanol, 55% aquiferous ethanol, 70% aquiferous ethanol carry out gradient and wash
De-, finally with 70% acetone eluting, collect each position eluent of 3 times of column volumes respectively, collection merging, 25% aquiferous ethanol, 35%
Aquiferous ethanol, 45% aquiferous ethanol and the eluent at each position of 55% aquiferous ethanol, the eluent that will merge, obtain ginkgo biloba leaf total yellow
Ketone extracting solution.
7. big Ye Fusejila wood cuttage breeding method as claimed in claim 1, it is characterised in that in step 4): indole fourth
Acid: naphthalene acetic acid: ABT6 root-inducing powder: the weight ratio of Folium Ginkgo total flavones extracting solution is 5:2:2:1.
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| CN106888890A (en) * | 2016-12-31 | 2017-06-27 | 孙明宗 | A kind of fig cuttage and seedling culture method |
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