CN111512942A - Polygonatum sibiricum asexual propagation method for improving emergence rate and strong seedling rate - Google Patents
Polygonatum sibiricum asexual propagation method for improving emergence rate and strong seedling rate Download PDFInfo
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- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
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- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/08—Alkali metal chlorides; Alkaline earth metal chlorides
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/14—Celastraceae [Staff-tree or Bittersweet family], e.g. spindle tree, bittersweet or thunder god vine
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
Abstract
A vegetative propagation method for sealwort capable of improving the rate of emergence and the rate of strong seedlings comprises the following technical steps. (1) The method comprises the following steps of (1) raw material pretreatment, (2) preparation of germination culture soil, (3) preparation of rooting culture solution, (4) preparation of seedling raising fertilizer, (5) germination culture of rhizoma polygonati stem blocks, (6) cluster seedling culture, and (7) seedbed culture. The emergence rate of the wound surface of the stem section of the polygonatum sibiricum in the method is higher than 97.5 percent; after the cultivation of the seedbed, the strong seedling rate of the sealwort seedlings which can be transplanted is higher than 95 percent.
Description
Technical Field
The invention relates to a polygonatum rhizome vegetative propagation method for improving the rate of emergence and the rate of strong seedlings, belonging to the technical field of Chinese herbal medicine cultivation.
Background
Rhizoma Polygonati (Polygonatum sibiricum) The polygonatum kingianum, the yellow chicken dish, the pennisetum purpureum, the conic gymnadenia rhizome, the tiger ginger and the rhizome of schefflera arboricola are perennial herbaceous plants of the polygonatum genus of the liliaceae family, are important traditional Chinese medicinal materials in China, and are also medicinal and edible articles.
Rhizoma Polygonati has the same shape as rhizoma Dioscoreae, can be eaten raw or stewed, and has effects of allaying hunger and promoting health. The rhizome of sealwort contains polysaccharide, glycan, flavone, lignin, alkaloid, amino acid and other substances, and modern pharmacological research shows that the sealwort has the effects of tonifying kidney and replenishing vital essence, nourishing yin and moistening dryness, resisting oxidation and aging, regulating human immunity, reducing blood fat, reducing blood sugar, inhibiting cholesterol, preventing atheromatosis, improving learning and memory capacity, resisting inflammation, resisting virus and the like, and is very beneficial to human bodies.
At present, with the medicinal value and the health care value of polygonatum being continuously recognized by people, the economic value of polygonatum is more and more prominent, the market demand is increased year by year, polygonatum is collected and utilized in large quantities, and the wild polygonatum resource is rapidly reduced to be about to be exhausted. Therefore, the method greatly develops artificial standard cultivation, becomes an effective measure for solving the contradiction between supply and demand of the polygonatum sibiricum market, and has more and more prominent significance for developing polygonatum sibiricum breeding technology.
The sealwort is artificially grown in a large scale, the sealwort seedlings are generally obtained by a tuber propagation mode at present, the propagation method can reduce the planting period, and the sealwort seedlings can be harvested in 3 years generally, but the method also has the problems of limited tuber bud points, less seedling quantity, unstable quality after planting and the like. At present, the sealwort planting seedling propagation becomes the technical bottleneck of artificial large-scale planting, and the development of a rapid propagation method with stable technology, short period, high emergence rate and high strong seedling rate is urgent.
Disclosure of Invention
The invention aims to provide a polygonatum rhizome asexual propagation method for improving the rate of emergence and the rate of strong seedlings.
In order to achieve the above objects and other related objects, the present invention provides the following technical solutions: a rhizoma polygonati vegetative propagation method for improving the rate of emergence and the rate of strong seedlings comprises the following steps:
step 1: mixing the gardenia leaves and the tripterygium wilfordii according to the mass ratio of 4-5:1 to obtain a mixture; mixing the mixture with purified water at a mass ratio of 1:5-8, placing in a stainless steel hot extraction device with a steam condensation collection device, boiling for extraction for 60-90min, filtering the extractive solution with a 80-100 mesh screen after extraction, and respectively collecting filtrate and residue; drying the filter residue by hot air at 80-90 ℃ until the water content is less than or equal to 8%, and then crushing to 40-60 meshes to obtain filter residue powder; drying the oil tea cake until the water content is less than or equal to 8%, and then crushing to 40-60 meshes to obtain oil tea cake powder;
step 2: uniformly mixing 70-80 parts by weight of plant ash, 10-15 parts by weight of filter residue powder and 10-15 parts by weight of oil tea cake powder to obtain budding culture soil;
and step 3: uniformly mixing 800 parts by weight of purified water of 750-;
and 4, step 4: uniformly mixing 20-30 parts by weight of calcium superphosphate, 20-30 parts by weight of filter residue powder and 50-55 parts by weight of oil tea cake powder to obtain a seedling raising fertilizer;
and 5: cleaning rhizoma Polygonati tubers to remove soil on the surface, wiping the surfaces of the rhizoma Polygonati tubers with alcohol for sterilization, then washing with sterile water, and draining to obtain clean rhizoma Polygonati tubers; cutting clean rhizome blocks of rhizoma Polygonati into stem segments with only one terminal bud, cutting a wound surface at the concave position of the upper stem root of the node in a disc shape, uniformly spraying 6-benzylamino adenine aqueous solution with the concentration of 5-10mg/L on the wound surface, and completely covering the wound surface with budding culture soil; then placing the stem section in a low-light environment with the temperature of 23-29 ℃ and the relative humidity of more than or equal to 60 percent for culturing for 21-35 days, and generating new buds at the wound surface of the stem section;
step 6: after the stem section wound surface generates new buds, the stem section is irrigated with rooting culture solution once every 7-9h, and is cultivated for 14-21d in a low-light environment with the temperature of 23-29 ℃ and the relative humidity of more than or equal to 60%, and the new buds can develop into cluster seedlings;
and 7: transplanting the cluster seedlings to a seedbed, adding seedling raising fertilizer accounting for 15-20% of the mass of the cluster seedlings into seedbed soil, and culturing for 30-45 days in a low-light environment to obtain the sealwort seedlings for transplanting.
The preferable technical scheme is as follows: in the extraction process of the step 1, the material is in an ultrasonic environment, the ultrasonic frequency is 25-35KHz, and the power density is 0.35-0.45w/cm2。
The preferable technical scheme is as follows: the gardenia leaves and the tripterygium wilfordii are dry products.
The preferable technical scheme is as follows: the illumination intensity of the weak light is 1000-.
Due to the application of the technical scheme, compared with the prior art, the invention has the advantages that:
1. according to the invention, gardenia leaves, tripterygium wilfordii and oil tea cakes are comprehensively utilized, and the budding culture soil, the rooting culture solution and the seedling raising fertilizer required by the vegetative propagation of polygonatum are obtained after ultrasonic-assisted extraction, drying, mixing and other operations.
2. The budding culture soil, the rooting culture solution and the seedling raising fertilizer are respectively used in stem wound surface budding, cluster seedling culture and seedbed culture of vegetative propagation of polygonatum sibiricum, provide nutrition for polygonatum sibiricum seedlings, have the effects of antibiosis and insect prevention, and can improve the rate of emergence and the rate of strong seedlings.
3. The emergence rate of the wound surface of the stem section of the polygonatum sibiricum in the method is higher than 97.5 percent; after the cultivation of the seedbed, the strong seedling rate of the sealwort seedlings which can be transplanted is higher than 95 percent.
Detailed Description
The following description of the embodiments of the present invention is provided for illustrative purposes, and other advantages and effects of the present invention will be apparent to those skilled in the art from the present disclosure.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise.
In describing the preferred embodiments, specific terminology may be employed for the sake of clarity; however, it is not intended that the disclosure herein be limited to the specific terminology so selected; and it is to be understood that each specific element includes all equivalent techniques that perform the same function, operate in a similar manner, and achieve a similar result.
The first embodiment is as follows: polygonatum sibiricum asexual propagation method for improving emergence rate and strong seedling rate
A vegetative propagation method for sealwort capable of improving the rate of emergence and the rate of strong seedlings comprises the following technical steps.
(1) Pretreatment of raw materials
Mixing the gardenia leaves and the tripterygium wilfordii according to the mass ratio of 4.5:1 to obtain a mixture of the gardenia leaves and the tripterygium wilfordii; mixing the mixture with purified water at a mass ratio of 1:6.5, and placing the mixture in a stainless steel hot extraction device with a steam condensation collection deviceBoiling and extracting in the device for 60-90min, wherein the material is in ultrasonic environment, the ultrasonic frequency is 30KHz, and the power density is 0.4w/cm2(ii) a After extraction, filtering the extracting solution by a 90-mesh screen, and respectively collecting filtrate and filter residue for later use. The gardenia leaves and the tripterygium wilfordii are dry products.
And drying the filter residue by adopting hot air at 85 ℃ until the water content is lower than 8%, and then crushing to 50 meshes to obtain filter residue powder for later use.
Drying the oil tea cake at vacuum low temperature under the conditions of vacuum gauge pressure of-0.08 to-0.10 Mpa and temperature of 55 to 65 ℃ until the water content is lower than 8 percent, and crushing to 50 meshes to obtain oil tea cake powder for later use.
(2) Preparing culture soil for budding
Uniformly mixing 75 parts by weight of plant ash, 12 parts by weight of the filter residue powder in the step (1) and 12 parts by weight of the oil tea cake powder in the step (1) to obtain mixed powder, namely the budding culture soil.
(3) Preparation of rooting culture solution
780 parts by weight of purified water, 220 parts by weight of the filtrate in (1), 1.3 parts by weight of potassium nitrate, 0.7 part by weight of ammonium sulfate, 1.3 parts by weight of monopotassium phosphate, 0.06 part by weight of magnesium sulfate and 0.045 part by weight of anhydrous calcium chloride were uniformly mixed to obtain a rooting culture solution.
(4) Preparation of seedling fertilizer
Uniformly mixing 25 parts by weight of calcium superphosphate, 25 parts by weight of the filter residue powder in the step (1) and 52 parts by weight of the oil tea cake powder in the step (1) to obtain mixed powder, namely the seedling raising fertilizer.
(5) Budding culture of rhizome of Polygonatum sibiricum
Selecting rhizoma Polygonati tubers without diseases and insect pests and with hidden bud spots, cleaning to remove surface soil, wiping the surfaces of the tubers with 75% alcohol fully for disinfection, then washing with sterile water fully, and draining to obtain clean rhizoma Polygonati tubers for later use.
Cutting clean rhizome blocks of polygonatum into stem segments only provided with one terminal bud, cutting a wound surface with the diameter of about 1.0cm at the concave position of the upper stem root of a nodule in a disc shape, uniformly spraying 6-benzylamino adenine solution with the concentration of 7.5mg/L on the wound surface, and completely covering the wound surface with sufficient budding culture soil in the step (2); and (3) placing the stem sections covered with the soil in a weak light environment with the temperature of 26 ℃ and the relative humidity of more than 60%, and culturing for 30d, so that more obvious buds can be observed on the wound surfaces of the stem sections.
(6) Culture of clump seedlings
After obvious new buds are observed at the wound surface of the stem section in the step (5), the stem section is watered with the rooting culture solution in the step (3) once every 8 hours, and the stem section is cultivated for 18 days in a low-light environment with the temperature of 26 ℃ and the relative humidity of more than 60 percent, so that the new buds can develop into clumpy seedlings with the length of more than 8 cm.
(7) Seedbed culture
Transplanting the cluster seedlings to a seedbed, adding seedling raising fertilizer accounting for 18 percent (4) of the mass of the cluster seedlings into seedbed soil, and culturing for 35 days at normal temperature in low light to obtain sealwort seedlings for transplanting.
The illumination intensity of the weak light is 1000-.
A rhizoma Polygonati vegetative propagation method for improving rate of emergence and strong seedling rate comprises the steps of increasing the rate of emergence of wound surface of stem segment of rhizoma Polygonati to 97.5%; after the cultivation of the seedbed, the strong seedling rate of the sealwort seedlings which can be transplanted is higher than 95 percent.
Example two: polygonatum sibiricum asexual propagation method for improving emergence rate and strong seedling rate
A rhizoma polygonati vegetative propagation method for improving the rate of emergence and the rate of strong seedlings comprises the following steps:
step 1: mixing gardenia leaves and tripterygium wilfordii according to the mass ratio of 4:1 to obtain a mixture; mixing the mixture with purified water at a mass ratio of 1:5, placing in a stainless steel hot extraction device with a steam condensation collection device, boiling for extraction for 60min, filtering the extractive solution with a 80-mesh screen, and collecting filtrate and residue respectively; drying the filter residue by hot air at 80 ℃ until the water content is less than or equal to 8%, and then crushing to 40 meshes to obtain filter residue powder; drying the oil tea cake until the water content is less than or equal to 8%, and then crushing to 40 meshes to obtain oil tea cake powder;
step 2: uniformly mixing 70 parts by weight of plant ash, 10 parts by weight of filter residue powder and 10 parts by weight of oil tea cake powder to obtain budding culture soil;
and step 3: uniformly mixing 750 parts by weight of purified water, 200 parts by weight of filtrate, 1.0 part by weight of potassium nitrate, 0.5 part by weight of ammonium sulfate, 1.0 part by weight of monopotassium phosphate, 0.05 part by weight of magnesium sulfate and 0.025 part by weight of anhydrous calcium chloride to obtain a rooting culture solution;
and 4, step 4: uniformly mixing 20 parts by weight of calcium superphosphate, 20 parts by weight of filter residue powder and 50 parts by weight of oil tea cake powder to obtain a seedling raising fertilizer;
and 5: cleaning rhizoma Polygonati tubers to remove soil on the surface, wiping the surfaces of the rhizoma Polygonati tubers with alcohol for sterilization, then washing with sterile water, and draining to obtain clean rhizoma Polygonati tubers; cutting clean rhizome blocks of rhizoma Polygonati into stem segments with only one terminal bud, cutting a wound surface at the concave position of the upper stem root of the node in a disc shape, uniformly spraying 6-benzylaminopurine aqueous solution with the concentration of 5mg/L on the wound surface, and completely covering the wound surface with budding culture soil; then, the stem section is placed in a low-light environment with the temperature of 23 ℃ and the relative humidity of more than or equal to 60 percent to be cultured for 21d, and the wound surface of the stem section generates new buds;
step 6: after new buds are generated on the wound surface of the stem section, the stem section is applied with rooting culture solution once every 7h, and the stem section is cultivated for 14d in a low-light environment with the temperature of 23 ℃ and the relative humidity of more than or equal to 60 percent, so that the new buds can develop into cluster seedlings;
and 7: transplanting the cluster seedlings to a seedbed, adding seedling raising fertilizer accounting for 15% of the mass of the cluster seedlings into seedbed soil, and culturing for 30d in a low-light environment to obtain the sealwort seedlings for transplanting.
The preferred embodiment is: in the extraction process of the step 1, the material is in an ultrasonic environment, the ultrasonic frequency is 25KHz, and the power density is 0.35w/cm2。
The preferred embodiment is: the gardenia leaves and the tripterygium wilfordii are dry products.
The preferred embodiment is: the illumination intensity of the weak light is 1000 lx.
Example three: polygonatum sibiricum asexual propagation method for improving emergence rate and strong seedling rate
A rhizoma polygonati vegetative propagation method for improving the rate of emergence and the rate of strong seedlings comprises the following steps:
step 1: mixing gardenia leaves and tripterygium wilfordii according to the mass ratio of 5:1 to obtain a mixture; mixing the mixture with purified water according to a mass ratio of 1:8, placing into a stainless steel hot extraction device with a steam condensation collection device, boiling and extracting for 90min, filtering the extracting solution through a 100-mesh screen after extraction is finished, and respectively collecting filtrate and filter residue; drying the filter residue by hot air at 90 ℃ until the water content is less than or equal to 8%, and then crushing to 60 meshes to obtain filter residue powder; drying the oil tea cake until the water content is less than or equal to 8%, and then crushing to 60 meshes to obtain oil tea cake powder;
step 2: uniformly mixing 80 parts by weight of plant ash, 15 parts by weight of filter residue powder and 15 parts by weight of oil tea cake powder to obtain budding culture soil;
and step 3: uniformly mixing 800 parts by weight of purified water, 250 parts by weight of filtrate, 1.5 parts by weight of potassium nitrate, 1.0 part by weight of ammonium sulfate, 1.5 parts by weight of monopotassium phosphate, 0.075 part by weight of magnesium sulfate and 0.05 part by weight of anhydrous calcium chloride to obtain a rooting culture solution;
and 4, step 4: uniformly mixing 30 parts by weight of calcium superphosphate, 30 parts by weight of filter residue powder and 55 parts by weight of oil tea cake powder to obtain a seedling raising fertilizer;
and 5: cleaning rhizoma Polygonati tubers to remove soil on the surface, wiping the surfaces of the rhizoma Polygonati tubers with alcohol for sterilization, then washing with sterile water, and draining to obtain clean rhizoma Polygonati tubers; cutting clean rhizome blocks of rhizoma Polygonati into stem segments with only one terminal bud, cutting a wound surface at the concave position of the upper stem root of the node in a disc shape, uniformly spraying 6-benzylaminopurine aqueous solution with the concentration of 10mg/L on the wound surface, and completely covering the wound surface with budding culture soil; then, the stem section is placed in a low-light environment with the temperature of 29 ℃ and the relative humidity of more than or equal to 60 percent to be cultured for 35d, and new buds are generated at the wound surface of the stem section;
step 6: after new buds are generated at the wound surface of the stem section, the stem section is applied with rooting culture solution once every 9h, and is cultivated for 21d in a low-light environment with the temperature of 29 ℃ and the relative humidity of more than or equal to 60 percent, so that the new buds can develop into cluster seedlings;
and 7: transplanting the cluster seedlings to a seedbed, adding seedling raising fertilizer accounting for 20% of the mass of the cluster seedlings into seedbed soil, and culturing for 30-45 days in a low-light environment to obtain the sealwort seedlings for transplanting.
The preferred embodiment is: in the extraction process of the step 1, the material is in an ultrasonic environment, the ultrasonic frequency is 35KHz, and the power density is 0.45w/cm2。
The preferred embodiment is: the gardenia leaves and the tripterygium wilfordii are dry products.
The preferred embodiment is: the illumination intensity of the weak light is 1500 lx.
The foregoing is illustrative of the preferred embodiment of the present invention and is not to be construed as limiting thereof in any way, and any modifications or variations thereof that fall within the spirit of the invention are intended to be included within the scope thereof.
Claims (4)
1. A rhizoma polygonati vegetative propagation method for improving the rate of emergence and the rate of strong seedlings is characterized in that: comprises the following steps:
step 1: mixing the gardenia leaves and the tripterygium wilfordii according to the mass ratio of 4-5:1 to obtain a mixture; mixing the mixture with purified water at a mass ratio of 1:5-8, placing in a stainless steel hot extraction device with a steam condensation collection device, boiling for extraction for 60-90min, filtering the extractive solution with a 80-100 mesh screen after extraction, and respectively collecting filtrate and residue; drying the filter residue by hot air at 80-90 ℃ until the water content is less than or equal to 8%, and then crushing to 40-60 meshes to obtain filter residue powder; drying the oil tea cake until the water content is less than or equal to 8%, and then crushing to 40-60 meshes to obtain oil tea cake powder;
step 2: uniformly mixing 70-80 parts by weight of plant ash, 10-15 parts by weight of filter residue powder and 10-15 parts by weight of oil tea cake powder to obtain budding culture soil;
and step 3: uniformly mixing 800 parts by weight of purified water of 750-;
and 4, step 4: uniformly mixing 20-30 parts by weight of calcium superphosphate, 20-30 parts by weight of filter residue powder and 50-55 parts by weight of oil tea cake powder to obtain a seedling raising fertilizer;
and 5: cleaning rhizoma Polygonati tubers to remove soil on the surface, wiping the surfaces of the rhizoma Polygonati tubers with alcohol for sterilization, then washing with sterile water, and draining to obtain clean rhizoma Polygonati tubers; cutting clean rhizome blocks of rhizoma Polygonati into stem segments with only one terminal bud, cutting a wound surface at the concave position of the upper stem root of the node in a disc shape, uniformly spraying 6-benzylamino adenine aqueous solution with the concentration of 5-10mg/L on the wound surface, and completely covering the wound surface with budding culture soil; then placing the stem section in a low-light environment with the temperature of 23-29 ℃ and the relative humidity of more than or equal to 60 percent for culturing for 21-35 days, and generating new buds at the wound surface of the stem section;
step 6: after the stem section wound surface generates new buds, the stem section is irrigated with rooting culture solution once every 7-9h, and is cultivated for 14-21d in a low-light environment with the temperature of 23-29 ℃ and the relative humidity of more than or equal to 60%, and the new buds can develop into cluster seedlings;
and 7: transplanting the cluster seedlings to a seedbed, adding seedling raising fertilizer accounting for 15-20% of the mass of the cluster seedlings into seedbed soil, and culturing for 30-45 days in a low-light environment to obtain the sealwort seedlings for transplanting.
2. The method for vegetative propagation of polygonatum sibiricum with improved emergence rate and strong seedling rate according to claim 1, wherein: in the extraction process of the step 1, the material is in an ultrasonic environment, the ultrasonic frequency is 25-35KHz, and the power density is 0.35-0.45w/cm2。
3. The method for vegetative propagation of polygonatum sibiricum with improved emergence rate and strong seedling rate according to claim 1, wherein: the gardenia leaves and the tripterygium wilfordii are dry products.
4. The method for vegetative propagation of polygonatum sibiricum with improved emergence rate and strong seedling rate according to claim 1, wherein: the illumination intensity of the weak light is 1000-.
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| CN105230484A (en) * | 2015-09-25 | 2016-01-13 | 马龙县兴裕农业生物科技开发有限公司 | Rapid breeding method of rhizoma polygonati |
| CN106386184A (en) * | 2016-08-27 | 2017-02-15 | 广西御汇堂生物科技有限公司 | Tea-oil tree cutting and grafting integral reproduction method |
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| CN110973571A (en) * | 2019-12-30 | 2020-04-10 | 安徽舒香食品有限公司 | Method for extracting fragrant substances from peppers |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105230484A (en) * | 2015-09-25 | 2016-01-13 | 马龙县兴裕农业生物科技开发有限公司 | Rapid breeding method of rhizoma polygonati |
| CN106386184A (en) * | 2016-08-27 | 2017-02-15 | 广西御汇堂生物科技有限公司 | Tea-oil tree cutting and grafting integral reproduction method |
| CN108893203A (en) * | 2018-06-26 | 2018-11-27 | 安徽山美生物科技有限公司 | A kind of granulation of Oil-tea-cake dust, which is leached, goes Residual oil technique |
| CN110973571A (en) * | 2019-12-30 | 2020-04-10 | 安徽舒香食品有限公司 | Method for extracting fragrant substances from peppers |
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