CN103828717A - Rapid propagating and seedling culturing method by virtue of Bletilla tubers - Google Patents

Rapid propagating and seedling culturing method by virtue of Bletilla tubers Download PDF

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Publication number
CN103828717A
CN103828717A CN201410074803.1A CN201410074803A CN103828717A CN 103828717 A CN103828717 A CN 103828717A CN 201410074803 A CN201410074803 A CN 201410074803A CN 103828717 A CN103828717 A CN 103828717A
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bletilla striata
illumination
bletilla
seedling
tubercle
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向华
唐克华
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ZHANGJIAGANG BENCAO SCIENCE & TECHNOLOGY Co Ltd
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ZHANGJIAGANG BENCAO SCIENCE & TECHNOLOGY Co Ltd
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Abstract

The invention provides a rapid propagating method of Bletilla. Bletilla seedlings are obtained through the steps of treatment of seedling culturing beds, selection and pretreatment of explants, cluster bud induction, Bletilla seedling root system culturing, Bletilla division culturing, final-period management and the like. The method is very suitable for industrial production, and has the characteristics of excellent formula effect, high bud ratio, short culture time, vigorous plant growth, high transplanting survival rate, strong operability, high application value, low investment, high yield and the like.

Description

Utilize bletilla striata stem tuber to carry out fast seedling raising method
Technical field
The present invention relates to the medicinal plant engineering field of growing seedlings, specifically a kind of bletilla striata stem tuber epicuticle that makes full use of organizes rapid induction to go out the breeding and seedling method of seedling.
Background technology
The Chinese traditional herbs history of existing thousands of years of China, is still widely used in China and many countries and regions so far.But because traditional Chinese herbal medicine acquisition methods is that to gather and consume a large amount of wild plant resources be cost, in the time gathering and consumption exceedes the regeneration capacity of natural resources, will inevitably cause the in imminent danger of species even to be become extinct.Along with the destruction day by day of natural ecological environment, also further cause the scarcity of resources of medicinal plant simultaneously.
The bletilla striata, have another name called give free of charge, indocalamus orchid, be the one that the orchid family bletilla belongs to, plant Latin Bletilla striata by name.Perennial herb, pseudobulb piece root shape, white, plumpness, has finger-like difference.Stem is sturdy, upright, high 30-60 centimetre.Leaf 3-6 piece, lanceolar or wide lanceolar, tip is point gradually, and base portion sheath shape is embraced stem.Raceme top is raw, sparse, has colored 3-8 piece, spends greatly and beauty aubergine.Petal 3, the lip Long Circle of falling ovum, dark 3 split, and there is wave-formed mouth at middle sliver edge, and side sliver part is coated stamen post.Sepal 3, petal-shaped.Capsule, cylindric, above, 6 vertical ribs are outstanding.Seed is tiny of dust.Late April-late May at florescence, in fructescence 11 months, the last ten-days period.There is mutation to spend the bletilla striata in vain, floral white, garden-variety still has indigo plant, Huang, pink isochrome.Conventional division propagation.Spring young leaves sprout before or autumn and winter overground part withered after, the old strain of turn up, cuts apart pseudobulb and divide and plant, every strain can divide 3-5 strain, every strain must be with terminal bud.Also can adopt seed propagation, but because seed is tiny, hypoplasia, needs to use medium aseptic inoculation, complicated operation, therefore seldom used.
Be mainly used in hemoptysis and spit blood, traumatism and bleeding, sore swollen toxin, chapped skin.Pulmonary tuberculosis hemoptysis, ulcer haemorrhage.Its bulb is containing bletilla colloid, starch, volatilization wet goods.Medicinal, have produce effects, the effect such as tonifying lung hemostasis, detumescence, external application controls that traumatic bleeding, carbuncle are swollen, scald, furunculosis etc.Spend beauty, cultivation is for viewing and admiring.From documents and materials, at present mainly to use bletilla striata propagation by corm, plantation, at good, the fertile sand loam of draining and humus loam, needs warm more dark and damp environment.
Publication number be CN101940158A Introduction To Cn Patent a kind of method of utilizing bletilla striata seeds Fast-propagation seedling, adopt iodophor solution soaking disinfection bletilla striata seeds, in intelligent climate box, induced bundle is sprouted the stage, adopt improved culture medium, and rationally adjust developmental temperature, illumination is implanted and is filled in the stainless steel disc or porcelain dish of matrix through the seedling of cultivating for 35-50 days, then puts into 20-26 ℃ of indoor hardening, within bletilla striata growth of seedling 3-4 month, enters field planting.Shorten the seedling seedling cycle, improved the survival rate of bletilla striata seedling large Tanaka, while having avoided former technology mercuric chloride solution sterilization bletilla striata seeds, human body is worked the mischief.But a lot of bletilla striata seeds ateliosis, bletilla striata seeds does not germinate substantially, and economic benefit can not get effective raising, is difficult to this technology of large-scale promotion.
Publication number be CN102283115A Introduction To Cn Patent a kind of method of Fast-propagation bletilla striata seedling: select bletilla striata mature seed as explant.2) after seed disinfection, be seeded to germination medium, 7 days left and right seed germinations, formed protocorm about 14 days, and cutting protocorm, is forwarded to Protocorm Multiplication medium.3) protocorm is forwarded to inducing clumping bud and proliferated culture medium.4) cutting Multiple Buds makes its amount reproduction, is forwarded to Rooting and hardening-off culture base after waiting Multiple Buds to grow up to the seedling of high about 3cm, in the time that seedling grows up to high approximately 7~10cm, transplants.Solve seedling that current bletilla striata vegetative propagation exists and carried the problems such as virus, deterioration of variety, effective output are low.The present invention can obtain the virus-free seedling of a large amount of sexual propagations under manual control condition, and compared with traditional modes of reproduction, output improves approximately 30%, and the demand and supply contraction of alleviating the bletilla striata is had to positive effect.Adopt this method can make the bletilla striata form planting scale, but its Cultivate administration is more difficult, implantation time also lengthens, and the cost of group training seedling own is high, and peasant economy benefit can not effectively improve, and is also difficult to this technology of large-scale promotion.
Publication number be CN102577814A Introduction To Cn Patent a kind of honeysuckle and the bletilla striata efficiently overlap cultivating method: the bletilla striata is interplanted below the medicinal materials such as honeysuckle, with becoming thoroughly decomposed, animal manure grass covers the bletilla striata, by the management of the operations such as fertilising, weeding, desinsection, results after plantation about 5 years.This production method utilizes same piece of land to produce two kinds of medicinal materials simultaneously, has improved cropping index.Save recruitment cost, production cost reduces, and output increases, and economic benefit significantly improves.But because tree-like honeysuckle has a certain impact to the daylighting of the bletilla striata, directly affect the utilization of the bletilla striata to fertilizer and moisture.Honeysuckle is plucked asynchronous with bletilla striata lepisphere stem expanding stage, also can affect bletilla striata squama expansion of corms, directly affects medicinal herb grower's economic benefit.
In sum, the low cost that is difficult to extensive development and reduces seed seedling of germination rate while adopting bletilla striata seeds to grow seedlings, and adopt the bletilla striata to organize training, and greatly promote the cost of group training seedling, be difficult to make to plant family and connect love, and be difficult to realize the economic benefit that improves peasant.Also be that documents and materials reported that utilization band bud bletilla striata stem tuber divided numerous, by the bletilla striata stem tuber with bud head separately, and the bletilla striata tubercle that there is no bud head is followed by the bletilla striata stem tuber of bud head, cause in planting process its seed costs higher, and do not have the bletilla striata tubercle proportion of bud head very large, and very possible dead and waste in planting process, but adopting every year can only make in this way to grow seedlings improves 6-8 doubly, can cause the not waste with sprout tuber stem of the bletilla striata.Therefore, in order to meet the medicinal demand day by day increasing, make full use of each tubercle of the bletilla striata, go out bletilla striata sprout for each tubercle rapid induction, effectively improve reproduction coefficient, reduce the price of growing seedlings, avoided utilizing bletilla striata seeds breeding emergence rate extremely low, cultivating seeds process is loaded down with trivial details, and growth rate is slow, utilize fast breeding technique, realizing artificial cultivation is best solution.
Up to now, at home and abroad there is not yet about the report that directly carries out the multiple bud heads of drug-induced formation for bletilla striata stem tuber on common seedling bed.We find out the method for a set of maturation finally by lot of experiments, have successfully realized the Fast-propagation of the bletilla striata, can go out a large amount of seedling by quickly breeding, provide possibility for realizing the industrialization artificial cultivation bletilla striata.The successful foundation of bletilla striata stem tuber fast breeding technique method, can provide seedling for the extensive artificial cultivation medicinal plant bletilla striata, for modern science and technology agricultural provides a kind of practicable exploration project.
Summary of the invention
Because the bletilla striata is by problems such as pseudobulb reproduction coefficient are too low, and operating procedure is many during by tissue culture technology, process is complicated and require sterile working environment and associative operation technical staff, while causing group to cultivate by training, have high input, the problems such as output is not high, therefore the object of this invention is to provide a kind of not high to nurturing an environment, drop into the bletilla striata method for quickly breeding low, reproduction coefficient is high, reach applicable suitability for industrialized production, formula effect is good, bud ratio is high, incubation time is short, plant strain growth is vigorous, workable, using value advantages of higher.
The bletilla striata piece root that utilizes of the present invention carries out fast seedling raising method, and its concrete operation step is as follows:
(1) processing of seedling bed: silt soil is placed on to lowermost layer, and silt layer height is 2-6cm, puts into Nutrition Soil on silt upper strata, and Nutrition Soil layer height is 5-8cm, sprays carbendazim solution and carries out sterilization;
(2) choosing and pretreatment of explant: select on the pseudobulb of the bletilla striata sprout as culture materials, bletilla striata piece root is removed to coring, again by bletilla striata tubercle respectively, clean through liquid detergent and running water, put under flowing water and rinse after 10-30 minute, be placed in 75% alcohol and process 10-30 second, cut after the part of explant variable color for subsequent use;
(3) grow thickly sprout induction: bletilla striata tubercle is being drawn two to three times at one side general surgery blade, the degree of depth reaches 1-3.5mm, inserted in the induced liquid of growing seedlings and process 1-10min, then bletilla striata tubercle is taken out and lain on seedling bed from induced liquid, the place of streaking with cutter upward, cultivation temperature 23-27 ℃, illumination every day 16 hours, intensity of illumination 1000LX-1500LX, humidity is controlled at 70-85%, cultivate 15-35 days, until there is sprout to grow;
(4) bletilla striata shoot root system cultivates: spray the culture fluid secondary altogether of growing seedlings, and interval 3-6 days, every mu is sprayed 10-35kg/ time, cultivation temperature 23-27 ℃, illumination every day 12-13 hour, intensity of illumination 900LX-1300LX, humidity is controlled at 70-85%, cultivates 15-25 days;
(5) bletilla striata plant division is cultivated: bletilla striata tubercle is taken out, with cutter by each bud separately, put into ash mixing 20-30min, be transplanted to again in Nutrition Soil cultivation temperature 23-27 ℃, illumination every day 8-12 hour, intensity of illumination 700-1000LX, humidity is controlled at 55-80%, transplants 20-30 days, and transplanting survival rate reaches more than 90%;
(6) final-period management: transplant behind land for growing field crops about annual April, 6-9 month covers with two-layer sunshade net, and adopt dropper mode to carry out moisturizing, after entering November, be incubated water conservation with mulch film, to about Second Year March, remove mulch film, adopt and carry out bletilla striata cultivation management with upper type; If generation black spot, with 1000 times of liquid sprinklings of 70% thiophanate methyl Wettable Powder; If there is root knot nematode disease harm, fill with and execute basin soil with 3% carbofuran Granules; If execute 1 time phosphate fertilizer when fertilizer deficiency to mid-August after spending.
In a specific embodiments, the compound method of 1% carbendazim solution described in step (1) is that powder of carbendazim is bought in the market that takes 1g, add the water of 99g to mix, the 0.2-1% carbendazim aqueous solution that the sprinkling consumption of every mu of seedling bed is 5-40kg.
In a specific embodiments, Nutrition Soil described in step (1) and (5), can make by oneself, its formula is garden mould 3-5 part, compost (farmers''s compost) 3-6 part, river sand 2-3 part, ash 1-2 part, mix, or direct market is bought " golden No. 3 " of Hangzhou Jinhai Agricultural Science and Technology Co., Ltd., and peasant household is from principal mode plug seedling substrate, or the happy farm cultivating soil of Lv Zhiyuan Co., Ltd of Zhengjiang City.
In a specific embodiments, the GA(gibberellin that the induced liquid of growing seedlings described in step (3) contains 0.1%-0.8%), the carbendazim of 0.2%-1%, the 6-benzyl aminoadenine of 0.1%-2.5%.
In a specific embodiments, the culture fluid of growing seedlings described in step (4) contains 0.01%-0.5%NAA(naa).
In another embodiment, in step (6), land for growing field crops humidity is controlled at 40-70%; Temperature is controlled at 10-30 ℃.
Technique effect:
(1) directly take bletilla striata stem tuber as culture materials, explant is drawn materials to be easier to, all can draw materials throughout the year, and quantity is many, employing stem tuber is culture materials, both guaranteed genetic stability, and avoided cultivating in tissue culture procedures the increase of algebraically simultaneously, differentiation seedling is out prone to the phenomenon of variation.
(2) the inventive method utilizes bletilla striata stem tuber to set up Fast-propagation, increases this technology path of blastogenesis root synchronization, only needs to form whole plant about 50 days, and in 4-6 month, thing obtains a large amount of whole plants, realizes the large-scale industrialized of the bletilla striata and grows seedlings.
(3) bletilla striata seedling-growing method that the inventive method obtains is simple, and greatly reduces seedling cost.This cultural method sprouts soon, and the rate of increase is high, and method is simple, does not need increase group training equipment, and production cost is low, can be mass, and using value is high.
(4) production of the bletilla striata can be carried out under manual control condition, is not subject to the restriction of the factors such as season, weather conditions and soil, can get rid of the invasion and attack of damage by disease and insect and the impact of residue of pesticide, strictly controls the quality of the bletilla striata.
(5) use fast breeding technique provided by the invention to obtain bletilla striata seedling, carry out artificial cultivation, transplanting survival rate is higher than group training seedling, reform of nature environment more fast.
(6) can preserve the germ plasm resource of the bletilla striata, be conducive to protect bletilla striata wild resource simultaneously, reduce the destruction to natural resources.
Embodiment
Below, the present invention will be further detailed with embodiment, but it is not limited to any or the similar example of these embodiment.
Embodiment 1
Silt soil is placed on to lowermost layer, and silt layer height is 2-6cm, puts into Nutrition Soil on silt upper strata, and Nutrition Soil layer height is 5-8cm, and every mu of 0.8% carbendazim solution that sprays 20kg carries out sterilization; Select sprout on the pseudobulb of the bletilla striata as culture materials, bletilla striata piece root to be removed to coring, then by bletilla striata tubercle respectively, clean through liquid detergent and running water, put under flowing water and rinse after 10-30 minute, be placed in 75% alcohol and process 10-30 second, cut after the part of explant variable color for subsequent use; Bletilla striata tubercle is drawn two to three times with general surgery blade in one side, the degree of depth reaches 2-3.5mm, inserted in the induced liquid of growing seedlings that contains 0.6% gibberellin, 0.6% carbendazim, 1.5%6-benzyl aminoadenine and process 9min, then bletilla striata tubercle is taken out and lain on seedling bed from induced liquid, the place of streaking with cutter upward, cultivation temperature 23-27 ℃, illumination every day 16 hours, intensity of illumination 1000LX-1500LX, humidity is controlled at 70-85%, cultivate 15-35 days, until there is sprout to grow.The culture fluid secondary altogether of growing seedlings that sprinkling contains 0.2%NAA, interval 3-6 days, every mu is sprayed 15kg/ time, spray altogether 30kg/ mu, cultivation temperature 23-27 ℃, illumination every day 12-13 hour, intensity of illumination 900LX-1300LX, humidity is controlled at 70-85%, cultivates 15-25 days.Bletilla striata tubercle is taken out, with cutter by each bud separately, put into ash mixing 20-30min, be transplanted to again in Nutrition Soil cultivation temperature 23-27 ℃, illumination every day 8-12 hour, intensity of illumination 700-1000LX, humidity is controlled at 55-80%, transplants 20-30 days, and transplanting survival rate reaches more than 90%.About annual April, transplant behind land for growing field crops, 6-9 month covers with two-layer sunshade net, and adopts dropper mode to carry out moisturizing, is incubated water conservation with mulch film after entering November, and land for growing field crops humidity is controlled at 40-70%; Temperature is controlled at 10-30 ℃, to about Second Year March, removes mulch film, adopts and carries out bletilla striata cultivation management with upper type; If generation black spot, with 1000 times of liquid sprinklings of 70% thiophanate methyl Wettable Powder; If there is root knot nematode disease harm, fill with and execute basin soil with 3% carbofuran Granules; If execute 1 time phosphate fertilizer when fertilizer deficiency to mid-August after spending.
Embodiment 2
Silt soil is placed on to lowermost layer, and silt layer height is 2-6cm, puts into Nutrition Soil on silt upper strata, and Nutrition Soil layer height is 5-8cm, and every mu of 0.6% carbendazim aqueous solution that sprays 30kg carries out sterilization; Select sprout on the pseudobulb of the bletilla striata as culture materials, bletilla striata piece root to be removed to coring, then by bletilla striata tubercle respectively, clean through liquid detergent and running water, put under flowing water and rinse after 10-30 minute, be placed in 75% alcohol and process 10-30 second, cut after the part of explant variable color for subsequent use; Bletilla striata tubercle is drawn two to three times with general surgery blade in one side, the degree of depth reaches 1-2.5mm, inserted in the induced liquid of growing seedlings that contains 0.8% gibberellin, 0.9% carbendazim, 2.0%6-benzyl aminoadenine and process 7min, then bletilla striata tubercle is taken out and lain on seedling bed from induced liquid, the place of streaking with cutter upward, cultivation temperature 23-27 ℃, illumination every day 16 hours, intensity of illumination 1000LX-1500LX, humidity is controlled at 70-85%, cultivate 15-35 days, until there is sprout to grow.The culture fluid secondary altogether of growing seedlings that sprinkling contains 0.1%NAA, interval 3-6 days, every mu is sprayed 30kg/ time, cultivation temperature 23-27 ℃, illumination every day 12-13 hour, intensity of illumination 900LX-1300LX, humidity is controlled at 70-85%, cultivates 15-25 days.Bletilla striata tubercle is taken out, with cutter by each bud separately, put into ash mixing 20-30min, be transplanted to again in Nutrition Soil cultivation temperature 23-27 ℃, illumination every day 8-12 hour, intensity of illumination 700-1000LX, humidity is controlled at 55-80%, transplants 20-30 days, and transplanting survival rate reaches more than 90%.About annual April, transplant behind land for growing field crops, 6-9 month covers with two-layer sunshade net, and adopts dropper mode to carry out moisturizing, is incubated water conservation with mulch film after entering November, and land for growing field crops humidity is controlled at 40-70%; Temperature is controlled at 10-30 ℃, to about Second Year March, removes mulch film, adopts and carries out bletilla striata cultivation management with upper type; If generation black spot, with 1000 times of liquid sprinklings of 70% thiophanate methyl Wettable Powder; If there is root knot nematode disease harm, fill with and execute basin soil with 3% carbofuran Granules; If execute 1 time phosphate fertilizer when fertilizer deficiency to mid-August after spending.
Embodiment 3
Silt soil is placed on to lowermost layer, and silt layer height is 2-6cm, puts into Nutrition Soil on silt upper strata, and Nutrition Soil layer height is 5-8cm, and every mu of 0.5% carbendazim solution that sprays 35kg carries out sterilization; Select sprout on the pseudobulb of the bletilla striata as culture materials, bletilla striata piece root to be removed to coring, then by bletilla striata tubercle respectively, clean through liquid detergent and running water, put under flowing water and rinse after 10-30 minute, be placed in 75% alcohol and process 10-30 second, cut after the part of explant variable color for subsequent use; Bletilla striata tubercle is drawn two to three times with general surgery blade in one side, the degree of depth reaches 1-2.0mm, inserted in the induced liquid of growing seedlings that contains 0.8% gibberellin, 0.5% carbendazim, 2.5%6-benzyl aminoadenine and process 7min, then bletilla striata tubercle is taken out and lain on seedling bed from induced liquid, the place of streaking with cutter upward, cultivation temperature 23-27 ℃, illumination every day 16 hours, intensity of illumination 1000LX-1500LX, humidity is controlled at 70-85%, cultivate 15-35 days, until there is sprout to grow.The culture fluid secondary altogether of growing seedlings that sprinkling contains 0.3%NAA, interval 3-6 days, every mu is sprayed 10kg/ time, cultivation temperature 23-27 ℃, illumination every day 12-13 hour, intensity of illumination 900LX-1300LX, humidity is controlled at 70-85%, cultivates 15-25 days.Bletilla striata tubercle is taken out, with cutter by each bud separately, put into ash mixing 20-30min, be transplanted to again in Nutrition Soil cultivation temperature 23-27 ℃, illumination every day 8-12 hour, intensity of illumination 700-1000LX, humidity is controlled at 55-80%, transplants 20-30 days, and transplanting survival rate reaches more than 90%.About annual April, transplant behind land for growing field crops, 6-9 month covers with two-layer sunshade net, and adopts dropper mode to carry out moisturizing, is incubated water conservation with mulch film after entering November, and land for growing field crops humidity is controlled at 40-70%; Temperature is controlled at 10-30 ℃, to about Second Year March, removes mulch film, adopts and carries out bletilla striata cultivation management with upper type; If generation black spot, with 1000 times of liquid sprinklings of 70% thiophanate methyl Wettable Powder; If there is root knot nematode disease harm, fill with and execute basin soil with 3% carbofuran Granules; If execute 1 time phosphate fertilizer when fertilizer deficiency to mid-August after spending.
Embodiment 4
Silt soil is placed on to lowermost layer, and silt layer height is 2-6cm, puts into Nutrition Soil on silt upper strata, and Nutrition Soil layer height is 5-8cm, and every mu of 0.45% carbendazim solution that sprays 40kg carries out sterilization; Select sprout on the pseudobulb of the bletilla striata as culture materials, bletilla striata piece root to be removed to coring, then by bletilla striata tubercle respectively, clean through liquid detergent and running water, put under flowing water and rinse after 10-30 minute, be placed in 75% alcohol and process 10-30 second, cut after the part of explant variable color for subsequent use; Bletilla striata tubercle is drawn two to three times with general surgery blade in one side, the degree of depth reaches 1.5-3.5mm, inserted in the induced liquid of growing seedlings that contains 0.4% gibberellin, 0.4% carbendazim, 2.0%6-benzyl aminoadenine and processed 7min, again bletilla striata tubercle is taken out and lain on seedling bed from induced liquid, the place of streaking with cutter upward, cultivation temperature 23-27 ℃, illumination every day 16 hours, intensity of illumination 1000LX-1500LX, humidity is controlled at 70-85%, cultivate 15-35 days, until there is sprout to grow.The culture fluid secondary altogether of growing seedlings that sprinkling contains 0.15%NAA, interval 3-6 days, every mu is sprayed 20kg/ time, cultivation temperature 23-27 ℃, illumination every day 12-13 hour, intensity of illumination 900LX-1300LX, humidity is controlled at 70-85%, cultivates 15-25 days.Bletilla striata tubercle is taken out, with cutter by each bud separately, put into ash mixing 20-30min, be transplanted to again in Nutrition Soil cultivation temperature 23-27 ℃, illumination every day 8-12 hour, intensity of illumination 700-1000LX, humidity is controlled at 55-80%, transplants 20-30 days, and transplanting survival rate reaches more than 90%.About annual April, transplant behind land for growing field crops, 6-9 month covers with two-layer sunshade net, and adopts dropper mode to carry out moisturizing, is incubated water conservation with mulch film after entering November, and land for growing field crops humidity is controlled at 40-70%; Temperature is controlled at 10-30 ℃, to about Second Year March, removes mulch film, adopts and carries out bletilla striata cultivation management with upper type; If generation black spot, with 1000 times of liquid sprinklings of 70% thiophanate methyl Wettable Powder; If there is root knot nematode disease harm, fill with and execute basin soil with 3% carbofuran Granules; If execute 1 time phosphate fertilizer when fertilizer deficiency to mid-August after spending.

Claims (6)

1. the bletilla striata piece root that utilizes of the present invention carries out fast seedling raising method, and its concrete operation step is as follows:
(1) processing of seedling bed: silt soil is placed on to lowermost layer, and silt layer height is 2-6cm, puts into Nutrition Soil on silt upper strata, and Nutrition Soil layer height is 5-8cm, sprays carbendazim solution and carries out sterilization;
(2) choosing and pretreatment of explant: select on the pseudobulb of the bletilla striata sprout as culture materials, bletilla striata piece root is removed to coring, again by bletilla striata tubercle respectively, clean through liquid detergent and running water, put under flowing water and rinse after 10-30 minute, be placed in 75% alcohol and process 10-30 second, cut after the part of explant variable color for subsequent use;
(3) grow thickly sprout induction: bletilla striata tubercle is being drawn two to three times at one side general surgery blade, the degree of depth reaches 1-3.5mm, inserted in the induced liquid of growing seedlings and process 1-10min, then bletilla striata tubercle is taken out and lain on seedling bed from induced liquid, the place of streaking with cutter upward, cultivation temperature 23-27 ℃, illumination every day 16 hours, intensity of illumination 1000LX-1500LX, humidity is controlled at 70-85%, cultivate 15-35 days, until there is sprout to grow;
(4) bletilla striata shoot root system cultivates: spray the culture fluid secondary altogether of growing seedlings, and interval 3-6 days, every mu is sprayed 10-35kg/ time, cultivation temperature 23-27 ℃, illumination every day 12-13 hour, intensity of illumination 900LX-1300LX, humidity is controlled at 70-85%, cultivates 15-25 days;
(5) bletilla striata plant division is cultivated: bletilla striata tubercle is taken out, with cutter by each bud separately, put into ash mixing 20-30min, be transplanted to again in Nutrition Soil cultivation temperature 23-27 ℃, illumination every day 8-12 hour, intensity of illumination 700-1000LX, humidity is controlled at 55-80%, transplants 20-30 days, and transplanting survival rate reaches more than 90%;
(6) final-period management: transplant behind land for growing field crops about annual April, 6-9 month covers with two-layer sunshade net, and adopt dropper mode to carry out moisturizing, after entering November, be incubated water conservation with mulch film, to about Second Year March, remove mulch film, adopt and carry out bletilla striata cultivation management with upper type; If generation black spot, with 1000 times of liquid sprinklings of 70% thiophanate methyl Wettable Powder; If there is root knot nematode disease harm, fill with and execute basin soil with 3% carbofuran Granules; If execute 1 time phosphate fertilizer when fertilizer deficiency to mid-August after spending.
2. according to the method for claim 1, the compound method of 1% carbendazim solution described in step (1) is that powder of carbendazim is bought in the market that takes 1g, add the water of 99g to mix, the 0.2-1% carbendazim aqueous solution that the sprinkling consumption of every mu of seedling bed is 5-40kg.
3. according to the method for claim 1, Nutrition Soil described in step (1) and (5), can make by oneself, and its formula is garden mould 3-5 part, compost (farmers''s compost) 3-6 part, river sand 2-3 part, ash 1-2 part, mixes.
4. according to the method for claim 1, the 6-benzyl aminoadenine of the gibberellin that the induced liquid of growing seedlings described in step (3) contains 0.1%-0.8%, the carbendazim of 0.2%-1%, 0.1%-2.5%.
5. according to the method for claim 1, the culture fluid of growing seedlings described in step (4) contains 0.01%-0.5% naa.
6. according to the method for claim 1, in step (6), land for growing field crops humidity is controlled at 40-70%; Temperature is controlled at 10-30 ℃.
CN201410074803.1A 2013-08-15 2014-03-03 Rapid propagating and seedling culturing method by virtue of Bletilla tubers Pending CN103828717A (en)

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CN2013103548507A CN103430847A (en) 2013-08-15 2013-08-15 Method for carrying out rapid breeding and seedling culture by utilizing tubers of bletilla striata
CN201310354850.7 2013-08-15
CN201410074803.1A CN103828717A (en) 2013-08-15 2014-03-03 Rapid propagating and seedling culturing method by virtue of Bletilla tubers

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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103975838A (en) * 2014-06-06 2014-08-13 普洱南药庄园科技有限公司 Rhizoma bletillae seedling transplanting method
CN104041276A (en) * 2014-06-13 2014-09-17 江苏茅山地道中药材种植有限公司 Pollution-free bletilla striata cultivation method
CN104686127A (en) * 2014-07-28 2015-06-10 江苏省中国科学院植物研究所 Rhizome cutting propagation method for gerradanthus macrorrhizus introduced from Africa
CN104686363A (en) * 2015-03-28 2015-06-10 邓文祥 Sterilized processing method for bletilla striata explants
CN105145104A (en) * 2015-10-15 2015-12-16 张家界本草科技有限公司 Method for segmenting bletilla striata tubers to perform seedling propagation and raising
CN105165368A (en) * 2015-10-15 2015-12-23 向君民 Method for rapidly breeding seedlings by using bletilla striata tubers
CN107173222A (en) * 2017-03-27 2017-09-19 河池乐康生态农业科技有限公司 A kind of wild bletilla striata quick breeding method for tissue culture
CN107241998A (en) * 2017-06-28 2017-10-13 金寨县金绿源特色中药材种植农场 A kind of greenhouse gardening bletilla striata final-period management method
CN108522184A (en) * 2018-01-17 2018-09-14 刘雅文 A kind of rapid propagation method for bletilla striata
CN113951118A (en) * 2021-10-18 2022-01-21 浙江省农业科学院 Simple cultivation method for rapid seedling emergence of bletilla ochracea

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103430847A (en) * 2013-08-15 2013-12-11 张家界本草科技有限公司 Method for carrying out rapid breeding and seedling culture by utilizing tubers of bletilla striata
CN103947433B (en) * 2014-05-12 2015-11-04 贵州省农作物品种资源研究所 A kind of bletilla group training bulb forming seedling through one step culture method and matrix thereof
CN104472202A (en) * 2014-12-31 2015-04-01 贵州省农作物品种资源研究所 Cultivation method for bletilla striata seed stems
CN105294207A (en) * 2015-11-12 2016-02-03 张家界本草科技有限公司 Bio-fertilizer applicable to cultivation of bletilla striata
CN105409540A (en) * 2015-11-30 2016-03-23 泸州市森奥中药材有限公司 Tuber breeding method for Bletilla striata
CN105746119B (en) * 2016-02-25 2018-11-02 宁蒗县富农中药材种植专业合作社 A kind of cultivation implantation methods of bletilla
CN106305056A (en) * 2016-08-18 2017-01-11 安徽省彩枫农林科技有限公司 Method for accelerating growth of rhizoma bletillae domestication seedlings to form adult seedlings
CN106305055A (en) * 2016-08-18 2017-01-11 安徽省彩枫农林科技有限公司 Domestication method for rhizoma bletillae seedlings
CN107750843A (en) * 2017-10-31 2018-03-06 广西沙田仙人滩农业投资有限公司 A kind of method of the efficiently plant bletilla striata
CN109197591A (en) * 2018-10-24 2019-01-15 河南云帮农业科技有限公司 A kind of excellent bletilla striata tissue culture method
CN109463238A (en) * 2018-12-19 2019-03-15 湖南新汇制药股份有限公司 A kind of breeding method of rhizoma polygonati stem tuber
CN111557241A (en) * 2020-04-26 2020-08-21 湖北省农业科学院中药材研究所 Rapid propagation method and application of bighead atractylodes rhizome seedlings
CN111903518A (en) * 2020-07-28 2020-11-10 安徽东方金桥农林科技股份有限公司 Rapid propagation method of bletilla striata plants by taking bletilla striata rhizome as explant

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030027161A (en) * 2001-09-13 2003-04-07 충청북도 (관리부서:충청북도 농업기술원) New cultivar of Bletilla striata 'CHUNG BUKJARAN'
CN101940158A (en) * 2009-07-09 2011-01-12 正安县绿野科技产业有限责任公司 Method for rapidly propagating plantlets by utilizing bletilla striata seeds
CN102283115A (en) * 2011-06-28 2011-12-21 陕西科技大学 Method for quickly reproducing bletilla striata seedlings
CN103430847A (en) * 2013-08-15 2013-12-11 张家界本草科技有限公司 Method for carrying out rapid breeding and seedling culture by utilizing tubers of bletilla striata

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030027161A (en) * 2001-09-13 2003-04-07 충청북도 (관리부서:충청북도 농업기술원) New cultivar of Bletilla striata 'CHUNG BUKJARAN'
CN101940158A (en) * 2009-07-09 2011-01-12 正安县绿野科技产业有限责任公司 Method for rapidly propagating plantlets by utilizing bletilla striata seeds
CN102283115A (en) * 2011-06-28 2011-12-21 陕西科技大学 Method for quickly reproducing bletilla striata seedlings
CN103430847A (en) * 2013-08-15 2013-12-11 张家界本草科技有限公司 Method for carrying out rapid breeding and seedling culture by utilizing tubers of bletilla striata

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103975838B (en) * 2014-06-06 2016-01-27 普洱南药庄园科技有限公司 A kind of bletilla method for transplanting
CN103975838A (en) * 2014-06-06 2014-08-13 普洱南药庄园科技有限公司 Rhizoma bletillae seedling transplanting method
CN104041276A (en) * 2014-06-13 2014-09-17 江苏茅山地道中药材种植有限公司 Pollution-free bletilla striata cultivation method
CN104686127A (en) * 2014-07-28 2015-06-10 江苏省中国科学院植物研究所 Rhizome cutting propagation method for gerradanthus macrorrhizus introduced from Africa
CN104686363A (en) * 2015-03-28 2015-06-10 邓文祥 Sterilized processing method for bletilla striata explants
CN104686363B (en) * 2015-03-28 2016-06-29 邓文祥 The asepticization processing method of the outer implant of the Pseudobulbus Bletillae (Rhizoma Bletillae)
CN105165368A (en) * 2015-10-15 2015-12-23 向君民 Method for rapidly breeding seedlings by using bletilla striata tubers
CN105145104A (en) * 2015-10-15 2015-12-16 张家界本草科技有限公司 Method for segmenting bletilla striata tubers to perform seedling propagation and raising
CN105145104B (en) * 2015-10-15 2016-09-21 张家界本草科技有限公司 Pseudobulbus Bletillae (Rhizoma Bletillae) tuber is utilized to carry out splitting breeding and seedling method
CN107173222A (en) * 2017-03-27 2017-09-19 河池乐康生态农业科技有限公司 A kind of wild bletilla striata quick breeding method for tissue culture
CN107241998A (en) * 2017-06-28 2017-10-13 金寨县金绿源特色中药材种植农场 A kind of greenhouse gardening bletilla striata final-period management method
CN108522184A (en) * 2018-01-17 2018-09-14 刘雅文 A kind of rapid propagation method for bletilla striata
CN113951118A (en) * 2021-10-18 2022-01-21 浙江省农业科学院 Simple cultivation method for rapid seedling emergence of bletilla ochracea
CN113951118B (en) * 2021-10-18 2023-02-17 浙江省农业科学院 Simple cultivation method for rapid seedling emergence of bletilla ochracea

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