CN103749129B - Method for asexually and rapidly propagating homalium hainanense - Google Patents
Method for asexually and rapidly propagating homalium hainanense Download PDFInfo
- Publication number
- CN103749129B CN103749129B CN201410017498.2A CN201410017498A CN103749129B CN 103749129 B CN103749129 B CN 103749129B CN 201410017498 A CN201410017498 A CN 201410017498A CN 103749129 B CN103749129 B CN 103749129B
- Authority
- CN
- China
- Prior art keywords
- cuttage
- homalium
- hainanense
- cutting
- days
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000789532 Homalium Species 0.000 title claims abstract description 51
- 230000001902 propagating Effects 0.000 title abstract 3
- 241000196324 Embryophyta Species 0.000 claims abstract description 35
- 201000010099 disease Diseases 0.000 claims abstract description 21
- 239000002420 orchard Substances 0.000 claims abstract description 15
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 7
- 239000003337 fertilizer Substances 0.000 claims description 47
- 239000002131 composite material Substances 0.000 claims description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 33
- 210000003608 Feces Anatomy 0.000 claims description 25
- 239000010871 livestock manure Substances 0.000 claims description 25
- 239000007921 spray Substances 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 24
- 238000011069 regeneration method Methods 0.000 claims description 21
- 239000002689 soil Substances 0.000 claims description 20
- 239000004576 sand Substances 0.000 claims description 15
- 238000010899 nucleation Methods 0.000 claims description 14
- VZJVWSHVAAUDKD-UHFFFAOYSA-N Potassium permanganate Chemical compound [K+].[O-][Mn](=O)(=O)=O VZJVWSHVAAUDKD-UHFFFAOYSA-N 0.000 claims description 12
- 238000005286 illumination Methods 0.000 claims description 12
- 239000002068 microbial inoculum Substances 0.000 claims description 12
- 230000002265 prevention Effects 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 241000238631 Hexapoda Species 0.000 claims description 8
- YYRMJZQKEFZXMX-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca+2].OP(O)(O)=O.OP(O)(O)=O YYRMJZQKEFZXMX-UHFFFAOYSA-N 0.000 claims description 8
- 239000002426 superphosphate Substances 0.000 claims description 8
- 239000002775 capsule Substances 0.000 claims description 7
- 235000013399 edible fruits Nutrition 0.000 claims description 7
- 102000018997 Growth Hormone Human genes 0.000 claims description 6
- 108010051696 Growth Hormone Proteins 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M Monopotassium phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 6
- 230000000249 desinfective Effects 0.000 claims description 6
- 238000003379 elimination reaction Methods 0.000 claims description 6
- 230000000749 insecticidal Effects 0.000 claims description 6
- 239000002917 insecticide Substances 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 239000002985 plastic film Substances 0.000 claims description 6
- 230000007226 seed germination Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 238000002054 transplantation Methods 0.000 claims description 6
- 238000009423 ventilation Methods 0.000 claims description 6
- 230000001058 adult Effects 0.000 claims description 4
- 239000011159 matrix material Substances 0.000 claims description 3
- 240000007072 Prunus domestica Species 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 230000035784 germination Effects 0.000 abstract description 5
- 230000018109 developmental process Effects 0.000 abstract description 4
- 230000002068 genetic Effects 0.000 abstract description 2
- 239000002023 wood Substances 0.000 abstract description 2
- 206010022114 Injury Diseases 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000000644 propagated Effects 0.000 abstract 1
- 229910052627 muscovite Inorganic materials 0.000 description 10
- 230000008929 regeneration Effects 0.000 description 4
- 230000004083 survival Effects 0.000 description 3
- 230000001488 breeding Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- -1 furniture Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 150000007530 organic bases Chemical class 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
Abstract
The invention discloses a method for asexually and rapidly propagating homalium hainanense. The method includes working procedures for collecting seeds, constructing cutting orchards, managing mother plants, collecting shoots for cutting, performing cutting and managing cutting seedlings, and particularly includes selecting excellent and healthy mature trees without disease and pest injury to be used as mother trees for seed collection; collecting the seeds and conventionally accelerating germination of the seeds; transplanting sprouts of the seeds on seedling growing beds to culture seedlings and using the seedling growing beds as the cutting orchards; pruning tops of the seedlings to promote germination; cultivating totally-lignified branches to be used as the cutting scions; performing cutting on the scions in container cups filled with media; cultivating cutting seedlings to acquire the excellent and healthy cutting seedlings of the homalium hainanense. The method for asexually and rapidly propagating the homalium hainanense has the advantages that the homalium hainanense can be asexually propagated by means of cutting, so that the seedlings keep original excellent genetic characters of female parents, large quantities of seedlings can be neatly outputted from the orchards, the problem of shortage in improved varieties due to massive development of the homalium hainanense can be solved, afforestation development of the homalium hainanense can be accelerated, living requirements of people on furniture made of precious wood can be met, and the method has an important significance.
Description
Technical field
The invention belongs to field of plant growing technology, relate to a kind of homalium hainanense Clonal regeneration method.
Background technology
Homalium hainanense (Homalium hainanense), the evergreen megaphanerophyte of Samydaceae Homalium, raw also known as mother, the height of tree can reach 40 meters.Suitable be born in southwest China to east, the Precious Timber Species in particularly Hainan Island, in Guangdong, the ground such as other area, Fujian, Guangxi is introduced a fine variety, grow fair, most area is blossomed and beared fruit, homalium hainanense is broad-leaved evergreen, tree body is graceful, and trunk is logical straight, is the excellent green tree species that a kind of suitable " four is other " is planted.Children slightly resistance toly to give shade age, and large tree light requirement is strong.Timber bronzing, texture is logical straight, wooden tough and tensile, and texture is fine and close, and flexible is shipbuilding, bridge, pillow material, furniture, water conservancy project and carpentry timber.Homalium hainanense usually with sowing and breeding nursery stock, but its capsule ripe after be easy to come off, seed lost vitality in 3 months, and germination rate is extremely low, and nursery germination percent only has about 30%, and the offspring of seed growing easily morphs, and nursery stock is uneven.
Summary of the invention
The present invention is in order to overcome the deficiencies in the prior art, and provide a kind of cost low, germination rate is high, is beneficial to the homalium hainanense Clonal regeneration method of Conservation and development homalium hainanense.
Technical scheme of the present invention is as follows:
A kind of homalium hainanense Clonal regeneration method, comprise seed collection, set up cutting orchard, maternal plant management, gather cuttings, cuttage and cuttage seeding management operation, select the excellent healthy and strong adult tree carpentery workshop seed collecting elite stand without damage by disease and insect, gather seed and carry out conventional vernalization, transplant and cultivate seedling as cutting orchard on nursery furrow, cut top to urge to sprout, cultivate full lignification branch and make cuttage fringe bar, by tloig-cutlage in installing in the container cup of matrix, cultivate cuttage seeding, obtain the homalium hainanense cuttage seeding of excellent stalwartness; Concrete operation step is as follows:
(1) seed collection: select the excellent healthy and strong adult tree carpentery workshop seed collecting elite stand without damage by disease and insect, when July ~ August or January in December to next year, capsule was ripe, seed collecting fruit, it is soft that indoor are accumulated to kind of shell corruption, clean with clear Xian of clear water, obtains seed;
(2) set up cutting orchard: by seed 65 ~ 75 DEG C of hot-water soaks, continue to soak 24h after naturally cooling, be sowed on clean wet husky bed, cover the fine sand of thick 2 ~ 3cm, sprinkle clear water, the shading screen of lid 65 ~ 75% degree of shading shades, and period keeps suitable humidity; Mix thoroughly with superphosphate, garbage disposal fertile to mix with nursery ground surface soil, play into nursery furrow stand-by: when seed germination has 2 pairs of complete leaves, little transplantation of seedlings is planted by specification to nursery furrow, the composite fertilizer that during plantation, dispenser routine uses, drench normal root water after planting, the shading screen of lid 65 ~ 75% degree of shading shades;
(3) maternal plant management: plant and open shading screen after 30 ~ 35 days and accept full exposure, fertilising interval time; Plant after 40 ~ 45 days, cut by profit short sprouting of pinching, regularly spray microbial inoculum diseases prevention routinely, during damaged by vermin, spray insecticide desinsection;
(4) cuttings is gathered: gather rudiment row culture cuttage cuttings, prune cuttage cuttings;
(5) cuttage: adopt yellow soil and fine sand as cutting medium, first loaded in cuttage vessel cup by cutting medium, then be placed on cuttage furrow by cuttage vessel cup, cuttage mass concentration the previous day is that the potassium permanganate of 2 ~ 5% is to the disinfection of cuttage furrow; Inserting in cutting medium after quickly dipped for section under cuttings somatotropin paste, the cuttage degree of depth is 2.5 ~ 4cm, normal root water of drenching;
(6) cuttage seeding management: cover film and shading screen after cuttage, the surrounding of film and shade net all uses dirt seal, in cutting bed, air humidity is 85 ~ 95%, open film and shading screen ventilation, trickle every 7 ~ 10 days, spray microbial inoculum and foliage fertilizer mixed liquor, after 0.5h, reseal film and shading screen; After cuttage rooting is stable, plastic film is raised, continues to shade, and in early before 11, at 4 in afternoon draws shading screen in, allows seedling adapt to illumination gradually later; Spike length to be inserted can raise shading screen after going out 3 pairs of young leaves, complete illumination, and then drenched every 8 ~ 10 days and execute composite fertilizer's liquid manure of using of routine that mass concentration is 0.4 ~ 0.8%, prevention and elimination of disease and pests, until the qualified homalium hainanense seedling growing up to complete stalwartness.
Above-described seed collecting elite stand selects plantation more than 25 years, and trunk leads to the straight excellent strain of ripe homalium hainanense.
Nursery furrow described in above step (2) are first turned over by cutting orchard to smash, and evenly sprinkles superphosphate 100Kg/ mu, garbage disposal fertilizer 200 ~ 300Kg/ mu, stir evenly, play into the nursery furrow that furrow face width is 1m with nursery ground surface soil.
Specification of planting described in above step (2) is the seeding row spacing of seedling is 15 ~ 20cm × 25 ~ 30cm.
The method of the composite fertilizer that the dispenser routine described in above step (2) uses first on nursery furrow, starts ditch when planting, open a line and plant a line, row ditch depth 10 ~ 20cm, by the amount of 2g ~ 4g/ strain, the composite fertilizer that routine uses evenly is sprinkled into row bottom of trench, stir with soil, earthing plantation seedling, plants rear pouring normal root water again.
Fertilising described in above step (3) is plantation after 15 ~ 18 days, drenches that to execute mass concentration be 1.5 ~ 2.0% Muscovite composite fertilizer liquid manure in the ranks, drenches that to execute a mass concentration is 2.0 ~ 2.5% Muscovite composite fertilizer liquid manure every 20 days ~ 25 days in the ranks later; Each collection fringe bar sprays the potassium dihydrogen phosphate promotion fringe bar lignification that mass concentration is 1 ~ 2% for first 10 days; After each collection fringe bar, it is 2.0 ~ 2.5% conventional composite fertilizer's liquid manure used that a mass concentration is executed in pouring in the ranks.
Above-described short sprouting of pinching retains after base portion up 3 ~ 5 pairs of leaves, cuts its upper part cut completely by profit.
When above-described cuttage cuttings is rudiment branch long 18 ~ 24cm, the thick 0.2 ~ 0.3cm of base portion complete lignification, cuts from rudiment branch base portion and obtain; It is the leaf retaining half on the top of cuttings that described cuttings is pruned, and the leaf of bottom all cuts off, and lower end is cut into angle shape from eye 1cm place.
Above-described cutting medium yellow soil 7 ~ 8 parts and fine sand 2 ~ 3 parts is mixed thoroughly to load in container cup.
Relative to prior art, the present invention has the following advantages and beneficial effect:
1, a kind of homalium hainanense Clonal regeneration of the present invention method, cuttage is used to carry out vegetative propagation homalium hainanense, both nursery stock had been made to keep maternal original excellent genetic character, the nursery stock in enormous quantities neatly going out garden can be provided again, solve the problem that homalium hainanense develops breeding shortage in a large number, the afforestation development and people of accelerating homalium hainanense are lived to precious solid wood furniture demand, there is extremely important meaning.
2, a kind of homalium hainanense Clonal regeneration of the present invention method, suitablely executes organic base manure and plantation maternal plant of topdressing, and makes that it grows soon, rudiment is many, improves reproduction coefficient, sprays foliage fertilizer and urge its lignification before gathering cuttings, raising cuttage survival rate.
3, a kind of homalium hainanense Clonal regeneration of the present invention method, cutting medium yellow soil mixes with fine sand, matrix is loosened moderate, and existing certain permeability has again certain preserve moisture and fertility ability, improves cuttage survival rate.
4, a kind of homalium hainanense Clonal regeneration of the present invention method, has the advantages that reproduction speed is fast, method simple, processing ease, survival rate are high, keeps excellent hereditary capacity, have good economic benefit, social benefit and ecological benefits.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Embodiment 1:
A kind of homalium hainanense Clonal regeneration method, selects plantation 25 years, and trunk is logical straight, excellent stalwartness does seed collecting elite stand without the excellent strain of ripe homalium hainanense of damage by disease and insect, when July, capsule was ripe, and seed collecting fruit, it is soft that indoor are accumulated to kind of shell corruption, clean with clear Xian of clear water, obtains seed.By seed 65 DEG C of hot-water soaks, continue to soak 24h after naturally cooling, be sowed on clean wet husky bed, cover the fine sand of thick 2cm, sprinkle clear water, the shading screen of lid 65% degree of shading shades, and period keeps suitable humidity.Turned over by cutting orchard and smash, evenly sprinkle superphosphate 100Kg/ mu, the fertile 200Kg/ mu of garbage disposal, stir evenly with nursery ground surface soil, playing into furrow face width is that the nursery furrow of 1m are stand-by.When seed germination has 2 pairs of complete leaves, be that 15cm × 25cm specification is planted the seeding row spacing by seedling in little transplantation of seedlings to nursery furrow, first on nursery furrow, ditch is started when planting, open a line and plant a line, row ditch depth 10cm, by the amount of 2g/ strain, the composite fertilizer that routine uses evenly is sprinkled into row bottom of trench, stir with soil, earthing plantation seedling, plants rear pouring normal root water again, and the shading screen of lid 65% degree of shading shades.
Plant and open shading screen after 30 days and accept full exposure, interval time applies fertilizer, namely in plantation after 15 days, in the ranks mass concentration is executed in pouring is 1.5% market buys Muscovite composite fertilizer liquid manure, and every 20 days, in the ranks a mass concentration was executed in pouring is later 2.0% market buys Muscovite composite fertilizer liquid manure; Each collection fringe bar sprays the potassium dihydrogen phosphate promotion fringe bar lignification that mass concentration is 1% for first 10 days; After each collection fringe bar, it is the 2.0% conventional composite fertilizer's liquid manure used that a mass concentration is executed in pouring in the ranks.Plant after 40 days, cut by profit short sprouting of pinching, be retain after base portion up 3 pairs of leaves, cut by profit and its upper part is cut completely.Regularly spray microbial inoculum diseases prevention to maternal plant routinely, during damaged by vermin, spray insecticide desinsection.
When the complete lignification of rudiment branch long 18 ~ 20cm, the thick 0.2cm of base portion, cut as cuttage cuttings from rudiment branch base portion; Retain the leaf of half on the top of cuttings, the leaf of bottom all cuts off, and lower end is cut into angle shape from eye 1cm place and obtains the cuttage cuttings after pruning.Yellow soil 7 parts and fine sand 2 parts are mixed thoroughly and obtain cutting medium, first loaded in cuttage vessel cup by cutting medium, then be placed on cuttage furrow by cuttage vessel cup, cuttage mass concentration the previous day is that the potassium permanganate of 2% is to the disinfection of cuttage furrow; Inserting in cutting medium after quickly dipped for section under cuttage cuttings somatotropin paste, the cuttage degree of depth is 2.5cm, normal root water of drenching.
Cover film and shading screen after cuttage, the surrounding of film and shade net all uses dirt seal, in cutting bed, air humidity is 85%, opens film and shading screen ventilation, trickle, sprays microbial inoculum and foliage fertilizer mixed liquor, reseal film and shading screen after 0.5h every 7 days; After cuttage rooting is stable, plastic film is raised, continues to shade, and in early before 11, at 4 in afternoon draws shading screen in, allows seedling adapt to illumination gradually later; Spike length to be inserted can raise shading screen after going out 3 pairs of young leaves, complete illumination, and then drenched every 8 days and execute composite fertilizer's liquid manure of using of routine that mass concentration is 0.4%, prevention and elimination of disease and pests, until the qualified homalium hainanense seedling growing up to complete stalwartness.
Embodiment 2:
A kind of homalium hainanense Clonal regeneration method, selects plantation 27 years, and trunk is logical straight, excellent stalwartness does seed collecting elite stand without the excellent strain of ripe homalium hainanense of damage by disease and insect, when August, capsule was ripe, and seed collecting fruit, it is soft that indoor are accumulated to kind of shell corruption, clean with clear Xian of clear water, obtains seed.By seed 70 DEG C of hot-water soaks, continue to soak 24h after naturally cooling, be sowed on clean wet husky bed, cover the fine sand of thick 2cm, sprinkle clear water, the shading screen of lid 70% degree of shading shades, and period keeps suitable humidity.Turned over by cutting orchard and smash, evenly sprinkle superphosphate 100Kg/ mu, the fertile 200Kg/ mu of garbage disposal, stir evenly with nursery earth's surface, playing into furrow face width is that the nursery furrow of 1m are stand-by.When seed germination has 2 pairs of complete leaves, be that 20cm × 25cm specification is planted the seeding row spacing by seedling in little transplantation of seedlings to nursery furrow, first on nursery furrow, ditch is started when planting, open a line and plant a line, row ditch depth 15cm, by the amount of 2g/ strain, the composite fertilizer that routine uses evenly is sprinkled into row bottom of trench, stir with soil, earthing plantation seedling, plants rear pouring normal root water again, and the shading screen of lid 70% degree of shading shades.
Plant and open shading screen after 35 days and accept full exposure, interval time applies fertilizer, namely in plantation after 15 days, in the ranks mass concentration is executed in pouring is 1.7% market buys Muscovite composite fertilizer liquid manure, and every 20 days, in the ranks a mass concentration was executed in pouring is later 2.0% market buys Muscovite composite fertilizer liquid manure; Each collection fringe bar sprays the potassium dihydrogen phosphate promotion fringe bar lignification that mass concentration is 1% for first 10 days; After each collection fringe bar, it is the 2.0% conventional composite fertilizer's liquid manure used that a mass concentration is executed in pouring in the ranks.Plant after 40 days, cut by profit short sprouting of pinching, be retain after base portion up 4 pairs of leaves, cut by profit and its upper part is cut completely.Regularly spray microbial inoculum diseases prevention to maternal plant routinely, during damaged by vermin, spray insecticide desinsection.
When the complete lignification of rudiment branch long 18 ~ 20cm, the thick 0.2cm of base portion, cut as cuttage cuttings from rudiment branch base portion; Retain the leaf of half on the top of cuttings, the leaf of bottom all cuts off, and lower end is cut into angle shape from eye 1cm place and obtains the cuttage cuttings after pruning.Yellow soil 7 parts and fine sand 3 parts are mixed thoroughly and obtain cutting medium, first loaded in cuttage vessel cup by cutting medium, then be placed on cuttage furrow by cuttage vessel cup, cuttage mass concentration the previous day is that the potassium permanganate of 3% is to the disinfection of cuttage furrow; Inserting in cutting medium after quickly dipped for section under cuttage cuttings somatotropin paste, the cuttage degree of depth is 3cm, normal root water of drenching.
Cover film and shading screen after cuttage, the surrounding of film and shade net all uses dirt seal, in cutting bed, air humidity is 90%, opens film and shading screen ventilation, trickle, sprays microbial inoculum and foliage fertilizer mixed liquor, reseal film and shading screen after 0.5h every 8 days; After cuttage rooting is stable, plastic film is raised, continues to shade, and in early before 11, at 4 in afternoon draws shading screen in, allows seedling adapt to illumination gradually later; Spike length to be inserted can raise shading screen after going out 3 pairs of young leaves, complete illumination, and then drenched every 9 days and execute composite fertilizer's liquid manure of using of routine that mass concentration is 0.5%, prevention and elimination of disease and pests, until the qualified homalium hainanense seedling growing up to complete stalwartness.
Embodiment 3:
A kind of homalium hainanense Clonal regeneration method, selects plantation 30 years, and trunk is logical straight, excellent stalwartness does seed collecting elite stand without the excellent strain of ripe homalium hainanense of damage by disease and insect, when December, capsule was ripe, and seed collecting fruit, it is soft that indoor are accumulated to kind of shell corruption, clean with clear Xian of clear water, obtains seed.By seed 70 DEG C of hot-water soaks, continue to soak 24h after naturally cooling, be sowed on clean wet husky bed, cover the fine sand of thick 3cm, sprinkle clear water, the shading screen of lid 75% degree of shading shades, and period keeps suitable humidity.Turned over by cutting orchard and smash, evenly sprinkle superphosphate 100Kg/ mu, the fertile 300Kg/ mu of garbage disposal, stir evenly with nursery earth's surface, playing into furrow face width is that the nursery furrow of 1m are stand-by.When seed germination has 2 pairs of complete leaves, be that 15cm × 30cm specification is planted the seeding row spacing by seedling in little transplantation of seedlings to nursery furrow, first on nursery furrow, ditch is started when planting, open a line and plant a line, row ditch depth 15cm, by the amount of 4g/ strain, the composite fertilizer that routine uses evenly is sprinkled into row bottom of trench, stir with soil, earthing plantation seedling, plants rear pouring normal root water again, and the shading screen of lid 75% degree of shading shades.
Plant and open shading screen after 35 days and accept full exposure, interval time applies fertilizer, namely in plantation after 16 days, in the ranks mass concentration is executed in pouring is 1.8% market buys Muscovite composite fertilizer liquid manure, and every 22 days, in the ranks a mass concentration was executed in pouring is later 2.3% market buys Muscovite composite fertilizer liquid manure; Each collection fringe bar sprays the potassium dihydrogen phosphate promotion fringe bar lignification that mass concentration is 2% for first 10 days; After each collection fringe bar, it is the 2.3% conventional composite fertilizer's liquid manure used that a mass concentration is executed in pouring in the ranks.Plant after 45 days, cut by profit short sprouting of pinching, be retain after base portion up 5 pairs of leaves, cut by profit and its upper part is cut completely.Regularly spray microbial inoculum diseases prevention to maternal plant routinely, during damaged by vermin, spray insecticide desinsection.
When the complete lignification of rudiment branch long 20 ~ 22cm, the thick 0.3cm of base portion, cut as cuttage cuttings from rudiment branch base portion; Retain the leaf of half on the top of cuttings, the leaf of bottom all cuts off, and lower end is cut into angle shape from eye 1cm place and obtains the cuttage cuttings after pruning.Yellow soil 8 parts and fine sand 2 parts are mixed thoroughly and obtain cutting medium, first loaded in cuttage vessel cup by cutting medium, then be placed on cuttage furrow by cuttage vessel cup, cuttage mass concentration the previous day is that the potassium permanganate of 4% is to the disinfection of cuttage furrow; Inserting in cutting medium after quickly dipped for section under cuttage cuttings somatotropin paste, the cuttage degree of depth is 3.5cm, normal root water of drenching.
Cover film and shading screen after cuttage, the surrounding of film and shade net all uses dirt seal, in cutting bed, air humidity is 95%, opens film and shading screen ventilation, trickle, sprays microbial inoculum and foliage fertilizer mixed liquor, reseal film and shading screen after 0.5h every 9 days; After cuttage rooting is stable, plastic film is raised, continues to shade, and in early before 11, at 4 in afternoon draws shading screen in, allows seedling adapt to illumination gradually later; Spike length to be inserted can raise shading screen after going out 3 pairs of young leaves, complete illumination, and then drenched every 9 days and execute composite fertilizer's liquid manure of using of routine that mass concentration is 0.6%, prevention and elimination of disease and pests, until the qualified homalium hainanense seedling growing up to complete stalwartness.
Embodiment 4:
A kind of homalium hainanense Clonal regeneration method, selects plantation 32 years, and trunk is logical straight, excellent stalwartness does seed collecting elite stand without the excellent strain of ripe homalium hainanense of damage by disease and insect, when January in next year, capsule was ripe, and seed collecting fruit, it is soft that indoor are accumulated to kind of shell corruption, clean with clear Xian of clear water, obtains seed.By seed 75 DEG C of hot-water soaks, continue to soak 24h after naturally cooling, be sowed on clean wet husky bed, cover the fine sand of thick 3cm, sprinkle clear water, the shading screen of lid 75% degree of shading shades, and period keeps suitable humidity.Turned over by cutting orchard and smash, evenly sprinkle superphosphate 100Kg/ mu, the fertile 300Kg/ mu of garbage disposal, stir evenly with nursery earth's surface, playing into furrow face width is that the nursery furrow of 1m are stand-by.When seed germination has 2 pairs of complete leaves, be that 20cm × 30cm specification is planted the seeding row spacing by seedling in little transplantation of seedlings to nursery furrow, first on nursery furrow, ditch is started when planting, open a line and plant a line, row ditch depth 20cm, by the amount of 4g/ strain, the composite fertilizer that routine uses evenly is sprinkled into row bottom of trench, stir with soil, earthing plantation seedling, plants rear pouring normal root water again, and the shading screen of lid 75% degree of shading shades.
Plant and open shading screen after 35 days and accept full exposure, interval time applies fertilizer, namely in plantation after 18 days, in the ranks mass concentration is executed in pouring is 2.0% market buys Muscovite composite fertilizer liquid manure, and every 25 days, in the ranks a mass concentration was executed in pouring is later 2.5% market buys Muscovite composite fertilizer liquid manure; Each collection fringe bar sprays the potassium dihydrogen phosphate promotion fringe bar lignification that mass concentration is 2% for first 10 days; After each collection fringe bar, it is the 2.5% conventional composite fertilizer's liquid manure used that a mass concentration is executed in pouring in the ranks.Plant after 45 days, cut by profit short sprouting of pinching, be retain after base portion up 5 pairs of leaves, cut by profit and its upper part is cut completely.Regularly spray microbial inoculum diseases prevention to maternal plant routinely, during damaged by vermin, spray insecticide desinsection.
When the complete lignification of rudiment branch long 22 ~ 24cm, the thick 0.3cm of base portion, cut as cuttage cuttings from rudiment branch base portion; Retain the leaf of half on the top of cuttings, the leaf of bottom all cuts off, and lower end is cut into angle shape from eye 1cm place and obtains the cuttage cuttings after pruning.Yellow soil 8 parts and fine sand 3 parts are mixed thoroughly and obtain cutting medium, first loaded in cuttage vessel cup by cutting medium, then be placed on cuttage furrow by cuttage vessel cup, cuttage mass concentration the previous day is that the potassium permanganate of 5% is to the disinfection of cuttage furrow; Inserting in cutting medium after quickly dipped for section under cuttage cuttings somatotropin paste, the cuttage degree of depth is 4cm, normal root water of drenching.
Cover film and shading screen after cuttage, the surrounding of film and shade net all uses dirt seal, in cutting bed, air humidity is 95%, opens film and shading screen ventilation, trickle, sprays microbial inoculum and foliage fertilizer mixed liquor, reseal film and shading screen after 0.5h every 10 days; After cuttage rooting is stable, plastic film is raised, continues to shade, and in early before 11, at 4 in afternoon draws shading screen in, allows seedling adapt to illumination gradually later; Spike length to be inserted can raise shading screen after going out 3 pairs of young leaves, complete illumination, and then drenched every 10 days and execute composite fertilizer's liquid manure of using of routine that mass concentration is 0.8%, prevention and elimination of disease and pests, until the qualified homalium hainanense seedling growing up to complete stalwartness.
Claims (9)
1. a homalium hainanense Clonal regeneration method, comprise seed collection, set up cutting orchard, maternal plant management, gather cuttings, cuttage and cuttage seeding management operation, it is waited to levy and is: select the excellent healthy and strong adult tree carpentery workshop seed collecting elite stand without damage by disease and insect, gather seed and carry out conventional vernalization, transplant and cultivate seedling as cutting orchard on nursery furrow, cut top to urge to sprout, cultivate full lignification branch and make cuttage fringe bar, by tloig-cutlage in installing in the container cup of matrix, cultivate cuttage seeding, obtain the homalium hainanense cuttage seeding of excellent stalwartness; Concrete operation step is as follows:
(1) seed collection: select the excellent healthy and strong adult tree carpentery workshop seed collecting elite stand without damage by disease and insect, when July ~ August or January in December to next year, capsule was ripe, seed collecting fruit, it is soft that indoor are accumulated to kind of shell corruption, clean with clear Xian of clear water, obtains seed;
(2) set up cutting orchard: by seed 65 ~ 75 DEG C of hot-water soaks, continue to soak 24h after naturally cooling, be sowed on clean wet husky bed, cover the fine sand of thick 2 ~ 3cm, sprinkle clear water, the shading screen of lid 65 ~ 75% degree of shading shades, and period keeps suitable humidity; Mix thoroughly with superphosphate, garbage disposal fertile to mix with nursery ground surface soil, play into nursery furrow stand-by; When seed germination has 2 pairs of complete leaves, little transplantation of seedlings is planted by specification to nursery furrow, the composite fertilizer that during plantation, dispenser routine uses, drenches normal root water after planting, and the shading screen of lid 65 ~ 75% degree of shading shades;
(3) maternal plant management: plant and open shading screen after 30 ~ 35 days and accept full exposure, fertilising interval time; Plant after 40 ~ 45 days, cut by profit short sprouting of pinching, regularly spray microbial inoculum diseases prevention routinely, during damaged by vermin, spray insecticide desinsection;
(4) cuttings is gathered: gather rudiment row culture cuttage cuttings, prune cuttage cuttings;
(5) cuttage: adopt yellow soil and fine sand as cutting medium, first loaded in cuttage vessel cup by cutting medium, then be placed on cuttage furrow by cuttage vessel cup, cuttage mass concentration the previous day is that the potassium permanganate of 2 ~ 5% is to the disinfection of cuttage furrow; Inserting in cutting medium after quickly dipped for section under cuttings somatotropin paste, the cuttage degree of depth is 2.5 ~ 4cm, normal root water of drenching;
(6) cuttage seeding management: cover film and shading screen after cuttage, the surrounding of film and shading screen all uses dirt seal, in cutting bed, air humidity is 85 ~ 95%, open film and shading screen ventilation, trickle every 7 ~ 10 days, spray microbial inoculum and foliage fertilizer mixed liquor, after 0.5h, reseal film and shading screen; After cuttage rooting is stable, plastic film is raised, continues to shade, and in early before 11, at 4 in afternoon draws shading screen in, allows seedling adapt to illumination gradually later; Spike length to be inserted can raise shading screen after going out 3 pairs of young leaves, complete illumination, and then drenched every 8 ~ 10 days and execute composite fertilizer's liquid manure of using of routine that mass concentration is 0.4 ~ 0.8%, prevention and elimination of disease and pests, until the qualified homalium hainanense seedling growing up to complete stalwartness.
2. a kind of homalium hainanense Clonal regeneration method according to claim 1, is characterized in that: described seed collecting elite stand selects plantation more than 25 years, and trunk leads to the straight excellent strain of ripe homalium hainanense.
3. a kind of homalium hainanense Clonal regeneration method according to claim 1, it is characterized in that: the nursery furrow described in step (2) are first turned over by cutting orchard to smash, evenly sprinkle superphosphate 100Kg/ mu, garbage disposal fertilizer 200 ~ 300Kg/ mu, stir evenly with nursery ground surface soil, play into the nursery furrow that furrow face width is 1m.
4. a kind of homalium hainanense Clonal regeneration method according to claim 1, is characterized in that: the specification of planting described in step (2) is the seeding row spacing of seedling is 15 ~ 20cm × 25 ~ 30cm.
5. a kind of homalium hainanense Clonal regeneration method according to claim 1, it is characterized in that: the method for the composite fertilizer that the dispenser routine described in step (2) uses first on nursery furrow, starts ditch when planting, open a line and plant a line, row ditch depth 10 ~ 20cm, by the amount of 2 ~ 4g/ strain, the composite fertilizer that routine uses evenly is sprinkled into row bottom of trench, stir with soil, then earthing plantation seedling, plant rear pouring normal root water.
6. a kind of homalium hainanense Clonal regeneration method according to claim 1, it is characterized in that: the fertilising described in step (3) is that plantation is after 15 ~ 18 days, in the ranks mass concentration is executed in pouring is 1.5 ~ 2.0% Russian composite fertilizer liquid manure, and every 20 days ~ 25 days, in the ranks a mass concentration was executed in pouring is later 2.0 ~ 2.5% Russian composite fertilizer liquid manure; Each collection fringe bar sprays the potassium dihydrogen phosphate promotion fringe bar lignification that mass concentration is 1 ~ 2% for first 10 days; After each collection fringe bar, it is 2.0 ~ 2.5% conventional composite fertilizer's liquid manure used that a mass concentration is executed in pouring in the ranks.
7. a kind of homalium hainanense Clonal regeneration method according to claim 1, is characterized in that: described short sprouting of pinching retains after base portion up 3 ~ 5 pairs of leaves, cuts its upper part cut completely by profit.
8. a kind of homalium hainanense Clonal regeneration method according to claim 1, is characterized in that: when described cuttage cuttings is rudiment branch long 18 ~ 24cm, the thick 0.2 ~ 0.3cm of base portion complete lignification, cuts and obtain from rudiment branch base portion; Described pruning cuttage cuttings is the leaf retaining half on the top of cuttings, and the leaf of bottom all cuts off, and lower end is cut into angle shape from eye 1cm place.
9. a kind of homalium hainanense Clonal regeneration method according to claim 1, is characterized in that: described cutting medium yellow soil 7 ~ 8 parts and fine sand 2 ~ 3 parts is mixed thoroughly to load in container cup.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410017498.2A CN103749129B (en) | 2014-01-15 | 2014-01-15 | Method for asexually and rapidly propagating homalium hainanense |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410017498.2A CN103749129B (en) | 2014-01-15 | 2014-01-15 | Method for asexually and rapidly propagating homalium hainanense |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103749129A CN103749129A (en) | 2014-04-30 |
| CN103749129B true CN103749129B (en) | 2015-05-13 |
Family
ID=50516589
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410017498.2A Expired - Fee Related CN103749129B (en) | 2014-01-15 | 2014-01-15 | Method for asexually and rapidly propagating homalium hainanense |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103749129B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106034712B (en) * | 2016-06-30 | 2019-04-19 | 四川省林业科学研究院 | A kind of alder spray single-bud cuttage method for culturing seedlings |
| CN107125118B (en) * | 2017-05-18 | 2019-11-19 | 湖北省林业科学研究院 | A kind of cultivation of Luotian weeping branch China fir base portion coppice shoot and cuttage and seedling culture method |
| CN108377830B (en) * | 2018-02-07 | 2020-04-07 | 广州建筑园林股份有限公司 | Method for cultivating clump-growing type large seedlings of red flower Tianmu |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072056A (en) * | 1992-01-22 | 1993-05-19 | 浙江省开化县林场 | Vegetative propagation technique for pine category |
| CN101317519A (en) * | 2007-06-06 | 2008-12-10 | 中国林业科学研究院林业研究所 | Light base material mesh bag container cutting propagation method for hard-radication wood species |
| CN101946665A (en) * | 2010-08-05 | 2011-01-19 | 江苏省林业科学研究院 | Method for cuttage and culture of seedling of Melia azedarach Linn |
| CN103168605A (en) * | 2013-04-11 | 2013-06-26 | 广西壮族自治区林业科学研究院 | Baeckea green branch cuttage vegetative rapid propagation method |
-
2014
- 2014-01-15 CN CN201410017498.2A patent/CN103749129B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1072056A (en) * | 1992-01-22 | 1993-05-19 | 浙江省开化县林场 | Vegetative propagation technique for pine category |
| CN101317519A (en) * | 2007-06-06 | 2008-12-10 | 中国林业科学研究院林业研究所 | Light base material mesh bag container cutting propagation method for hard-radication wood species |
| CN101946665A (en) * | 2010-08-05 | 2011-01-19 | 江苏省林业科学研究院 | Method for cuttage and culture of seedling of Melia azedarach Linn |
| CN103168605A (en) * | 2013-04-11 | 2013-06-26 | 广西壮族自治区林业科学研究院 | Baeckea green branch cuttage vegetative rapid propagation method |
Non-Patent Citations (4)
| Title |
|---|
| 不同季节对母生嫁接成活率的影响;吴坤明等;《热带林业科技》;19781231(第4期);第5～9页 * |
| 母生培育技术的研究初报;造林组珍贵树种培育专题;《热带林业科技》;19730402(第1期);第1～9页 * |
| 母生的培育技术;热带林业科学研究站;《林业科技通讯》;19740715(第7期);第7～8页 * |
| 红花天料木扦插育苗技术研究;薛杨等;《热带林业》;20090615;第37卷(第2期);第26+29页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103749129A (en) | 2014-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102960172B (en) | Short-spike and root-grafting rapid propagation method for camellia azalea | |
| CN102475020B (en) | Northern blueberry-growing method | |
| CN103430803B (en) | Shaping cultivation method of peach tree shape with three boughs and one center truck | |
| CN103430805B (en) | Shaping cultivation method of peach tree shape with two boughs and one center truck | |
| CN104106382B (en) | The propagation method of a kind of mao of purple camellia grafting Ta Mu island Camellia nitidissima | |
| CN101773038B (en) | Cutting propagation method of plukenetia volubilis linneo | |
| CN105557425B (en) | A kind of method of Chinese chestnut skeleton branch baldness with multiple spot grafting bearing basal shoot | |
| CN105359784A (en) | Method for high-yield planting of sweet potatoes | |
| CN103749238A (en) | Variety breeding and early-season high-yield cultivation technology for actinidia arguta | |
| CN105013808B (en) | A kind of method using mulberry tree cadmium pollution soil repair | |
| CN104082007A (en) | Cuttage breeding method for annona squamosa linn | |
| CN109220465A (en) | A kind of cultivation and pruning method of Chinese pricklyash | |
| CN103918459B (en) | One seed manure ox tree cuttage breeding method | |
| CN105145305B (en) | A kind of water planting method for culturing seedlings of the big tree needle-leaved bundle of wet-land pine tree | |
| CN103798054A (en) | High-yield high-efficiency cultivation mode for interplanting good mulberry with konjac | |
| CN106069535A (en) | A kind of method improving seed of Fructus Vitis viniferae planting percent | |
| CN103749129B (en) | Method for asexually and rapidly propagating homalium hainanense | |
| CN103493678B (en) | Horse chestnut seedling-cultivating method | |
| CN107409670A (en) | A kind of tobacco floating seed rearing pulls out method for transplanting under plant film | |
| CN101444166A (en) | Easy and quick propagating method for lance asiabell root | |
| CN105453983A (en) | Sowing propagation method for oriental arborvitae | |
| CN105532395A (en) | Method for using mixed light medium for subprostrate sophora twig cutting seedling raising, and application | |
| CN104686173A (en) | Method for jointly planting osmunda and loquat in farmland | |
| CN104938181A (en) | Efficient macadimia nut cultivation method | |
| CN111727753B (en) | Method for quickly growing seedlings and cultivating golden camellia |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150513 Termination date: 20180115 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |