CN108718739A - Golden Chinese scholartree cuttage and seedling culture method - Google Patents
Golden Chinese scholartree cuttage and seedling culture method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
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- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/14—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing aromatic radicals
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Abstract
The invention belongs to golden Chinese scholartree reproduction technique fields, and in particular to a kind of gold Chinese scholartree cuttage and seedling culture method.For golden Chinese scholartree graft seedling growth, time-consuming, of high cost, the low problem of cuttage and seedling culture survival rate, and the present invention provides a kind of golden Chinese scholartree cuttage and seedling culture method, includes the following steps:A, smooth cuttage seedbed prepares Nutrition Soil, Nutrition Soil is overlying on cuttage seedbed, forms cutting medium;B, choose golden Chinese scholartree branch, be cut into the cuttings of long 10~12cm, cuttings sterilizes be placed on and dip in 2~3s in the IAA solution of 800~1200ppm after, cuttage is sprinkled profoundly water in matrix after cuttage;C, it is 18~25 DEG C that air themperature is controlled after cuttage, and air humidity is 40~60%;In cuttage 40~50 days, control soil humidity is 70~75%, and after cuttage 40~50 days, control soil humidity is 55~65%.The method of the present invention makes golden Chinese scholartree cuttage survival rate be increased to 80% or so by 5%, reduces seedling raise period, has saved production cost, provides the foundation, has great importance for golden Chinese scholartree cuttage and seedling culture.
Description
Technical field
The invention belongs to golden Chinese scholartree reproduction technique fields, and in particular to a kind of gold Chinese scholartree cuttage and seedling culture method.
Background technology
Golden Chinese scholartree scientific name gold branch Chinese scholartree, Latin literary fame are (Sophora japonica cv.Golden Stem), alias gold branch
Chinese scholar tree etc..Golden Chinese scholartree is Rosales pulse family Sophora deciduous tree, belongs to one of mutation of Chinese scholar tree, and tree stem, branch are golden yellow, especially
In winter, this golden yellow is denseer, more gorgeous, and unique style is full of the elegance of gardens woody flower, has very high watch
Value.It is that one of seeds are commonly used in afforestation.
Not only there is golden Chinese scholartree ornamental value, leaf to be also rich in the ingredients such as rutin in spending, widely used.Existing gold Chinese scholartree kind
Plant is mainly distributed on the ground such as Guangxi, Yunnan, Sichuan, and plantation amount is little.This is mainly by golden Chinese scholartree propagation method complexity, survival rate
Not high limitation.
As long as existing gold Chinese scholartree method for culturing seedlings carries out scion grafting as stock using Chinese scholar tree or bud connects using grafting.Scion grafting is using slotting
Bark graft, before grafting, growth selection enriches, and the annotinous branch of no disease and pests harm, diameter 1cm or so does scion, and cutting back is at 10cm
Left and right is sealed with wax, and to prevent moisture loss, then sand storage is spare in shady and cool lee side, mid or late April, after Chinese scholar tree germinates, selection
The more straight Chinese scholar tree seedlings of transverse diameter 3cm or more, trunk are grafted after cutting strunk in place.The scion lower end bud back side is first whittled into long 3
The bevel of~5cm, bevel wants straight and is more than medulla, then long bevel rear end is whittled into the small inclined-plane of 0.5~0.8cm.?
At Chinese scholar tree cutting strunk, Chinese scholar tree cortex is vertically cut an osculum by the smooth straight place of choosing, and length is the 1/2~2/ of the long bevel of scion
3, scion is inserted among notch xylem and bast, long bevel makes scion back side alignment cuts hit exactly towards xylem,
Bevel " is left white " 0.3~0.4cm, can connect 2~3 scion according to Chinese scholar tree rugosity, and be uniformly distributed, after scion connects, with wide 5cm
The plastic cloth of left and right, wound is tied up sternly.
Bud connects, and is connect using rhizosphere bud, and clip gold Chinese scholartree annotinous branch does scion, removes spare after compound leaf;From scion branch
It is cut at 1~1.5cm of top of bud, slightly band xylem, which is directly put down downwards, cuts, until 1.5~2cm below bastem, laterally tiltedly partial application takes
Lower bud tablets.Then the stock of the thick 0.5cm or more of selection ground diameter, smoothly locates in stock away from ground 5cm or so windward side, from the top down
A section homogeneously worked as with piece length and width of buddering is cut, the lateral tiltedly partial application in lower end, which is removed, chips, and is connect with bud tablets are inserted into stock
Mouthful.Bevel alignment forming layer is tightly attached on stock bevel.With the plastic film strip bondage of thick 0.03cm, width 1.2cm or so, wound
It all twines and sternly fraps.If the summer connects, after 15 days, the plastic film near bud is scratched using knife, bud is made to be exposed, with
So that young sprout pumping is come out, while checking survival rate, mending again for not taking over a job connects.
The engrafting method of above-mentioned gold Chinese scholartree cultivates stock and about needs 3 years, and grafting needs 3~6 months, needs 4 years or so altogether, consumes
Duration;Limited amount is grafted, reproduction speed is slow;And manpower and materials consuming is more, of high cost, is also easy to restricted to time.
Therefore, the method for above-mentioned grafting is not suitable for the biological control of golden Chinese scholartree, is badly in need of finding a kind of easy, time-consuming gold short, at low cost
Chinese scholartree method for culturing seedlings.
The cuttage technique of plant is the technology that a kind of root, stem or leaf using plant carries out vegetative propagation, but golden Chinese scholartree due to
Its branch is not likely to produce callus, and leads to difficulty of taking root, and cuttage survival rate is extremely low, can not scale utilization, existing skill
Art yet there are no the high golden Chinese scholartree cottage method of survival rate.
Invention content
The technical problem to be solved in the present invention is:Time-consuming, of high cost for golden Chinese scholartree graft seedling growth, and cuttage and seedling culture survival rate is low
The problem of.
The present invention solve above-mentioned technical problem technical solution be:There is provided a kind of survival rate high golden Chinese scholartree cuttage and seedling culture side
Method.This approach includes the following steps:
A, smooth cuttage seedbed prepares Nutrition Soil, Nutrition Soil is overlying on cuttage seedbed, forms cutting medium;The battalion
It is that soil, turf and vermiculite are formulated by weight 45~50 ︰, 40~45 ︰ 5~15 to support soil;
B, healthy and strong annual golden Chinese scholartree branch to be chosen, is cut into the cuttings of long 10~12cm, cuttings disinfection is placed on 800~
After dipping in 2~3s in the IAA solution of 1200ppm, cuttage is sprinkled profoundly water in matrix after cuttage;
C, it is 18~25 DEG C that air themperature is controlled after cuttage, and air humidity is 40~60%;In cuttage 40~50 days, control
Soil humidity is 70~75%, and after cuttage 40~50 days, control soil humidity is 55~65%.
Wherein, in above-mentioned golden Chinese scholartree cuttage and seedling culture method, soil and turfy soil described in step a cross 80 mesh sieve.
Wherein, in above-mentioned golden Chinese scholartree cuttage and seedling culture method, Nutrition Soil described in step a first carries out desinsection before being overlying on seedbed and kills
Bacterium is handled.
Further, after the processing of Nutrition Soil pesticide-germicide is admixes Nutrition Soil using pesticide-germicide medicine described in step a, with modeling
Expect that film covers 3~5 days.
Further, the pesticide-germicide medicine is the mixture of phoxim emulsifiable concentrate and carbendazim.
Further, the pesticide-germicide medicament preparation is 50% 100~165mL of phoxim emulsifiable concentrate and carbendazim powder
10~15g of agent is watered 5~7.5kg and is prepared.50kg Nutrition Soils are added in the above-mentioned pesticide-germicide medicine prepared when use
In, it is uniformly mixed.
Wherein, in above-mentioned golden Chinese scholartree cuttage and seedling culture method, the Nutrition Soil described in step a be covered in the thickness on seedbed be 10~
15cm。
Wherein, in above-mentioned golden Chinese scholartree cuttage and seedling culture method, cuttings described in step b is cut into upper end flat mouth, the shape of lower end angle,
40~50 degree of angle angle.
Wherein, in above-mentioned golden Chinese scholartree cuttage and seedling culture method, cuttings disinfection described in step b uses 500~800 times of carbendazim solutions
Immersion 16~for 24 hours.
Wherein, in above-mentioned golden Chinese scholartree cuttage and seedling culture method, cuttings described in step b is oblique cutting, cuttings and matrix when being inserted into matrix
Plane forms 40~50 degree of angle.
Wherein, in above-mentioned golden Chinese scholartree cuttage and seedling culture method, 4~5cm of oblique cutting insertion depth described in step b, each cuttings interval 5
~6cm.
Wherein, in above-mentioned golden Chinese scholartree cuttage and seedling culture method, vermiculite is replaced by perlite described in step a.
Beneficial effects of the present invention are:
The present invention provides a kind of golden Chinese scholartree cottage method, using the Nutrition Soil especially prepared, with the use of carbendazim solution,
IAA solution treatment cuttage branches, can improve the cuttage survival rate of golden Chinese scholartree, make golden Chinese scholartree cuttage survival rate 80% or so;Meanwhile
Using the method for cuttage of the present invention, growing-seedling period is fast, and 3~4 months i.e. portable crop field from cuttage day, reproduction speed is than grafting
Method improves nearly 10 times;Cuttage quantity is big, and a scion can grow up to a golden Chinese scholartree, than the Jin Huaijie used in engrafting method
Fringe is few, at low cost;Cuttage will not be by seasonal effect, as long as condition properly can be carried out cuttage.Therefore, cuttage side of the invention
Method is spent human and material resources less, reproduction speed is fast, has apparent economic benefit.
Description of the drawings
Fig. 1 show the cuttings of 1 gold medal Chinese scholartree cuttage of embodiment;
Fig. 2 show the Jin Huaimiao of 1 rigid cuttage of embodiment;
Fig. 3 show the golden Chinese scholartree cuttage seeding that embodiment 1 forms callus;
Fig. 4 show the golden Chinese scholartree cuttage seeding that embodiment 1 grows new root;
Fig. 5 show the golden Chinese scholartree cuttage seeding that embodiment 1 survives.
Specific implementation mode
The present invention provides a kind of high golden Chinese scholartree cuttage and seedling culture methods of survival rate, include the following steps:
A, smooth cuttage seedbed prepares Nutrition Soil, Nutrition Soil is overlying on cuttage seedbed, forms cutting medium;The battalion
It is that soil, turf and vermiculite are formulated by weight 45~50 ︰, 40~45 ︰ 5~15 to support soil;
B, healthy and strong annual golden Chinese scholartree branch to be chosen, is cut into the cuttings of long 10~12cm, cuttings disinfection is placed on 800~
After dipping in 2~3s in the IAA solution of 1200ppm, cuttage is sprinkled profoundly water in matrix after cuttage;
C, it is 18~25 DEG C that air themperature is controlled after cuttage, and air humidity is 40~60%;In cuttage 30~40 days, control
Soil humidity is 70~75%, and after cuttage 40~45 days, control soil humidity is 55~65%.
Wherein, in above-mentioned golden Chinese scholartree cuttage and seedling culture method, the Nutrition Soil described in step a is soil, turf and perlite by weight
Amount is formulated than 45~50 ︰, 40~45 ︰ 5~15.The present invention prepares nutrition using soil, turf and vermiculite (or perlite)
Soil, soil dosage are 45~50%, and soil is nature soil, are golden Chinese scholartree seedling after cuttage wherein containing nutriments such as nitrogen, phosphorus, potassium
Nutrition being absorbed from soil, basis being provided, the soil of addition 45~50% not only can keep matrix loose, but also reduce and be produced into
This, it is unloose that soil matches excessive matrix, and branch is taken root more difficult, and proportioning is very few, and the additive amounts such as turf, vermiculite increase, raw
Cost is produced to increase.Soil used in the present invention, it is desirable that the granularity of soil and turf all cross 80 mesh screens, ensure that matrix granularity compared with
Carefully, it is conducive to keep water supply in media, is also conducive to the cuttage of branch, the friction injury of notch and matrix when mitigating branch cutting.
In order to increase the nutritional ingredient of cutting medium, turf of the present invention in soil with addition of 40~45%, turf is
The through a long times such as weeds rot to be prepared, and have good permeability and moisture retention, rich in nutrition content.Additive amount 40~
45% turf can improve the gas porosity of matrix, make it have preferable permeability and moisture retention, add excessive increase and produce
Cost adds the very few gas porosity that matrix is not achieved, is unfavorable for branch and takes root, and in order to ensure the gas porosity of matrix, improves cuttage
Survival rate, at the same on the basis of reducing production cost, the turf of addition 40~45%, and preferably can guarantee that turf crosses 80 mesh
Sieve.In order to increase the permeability and water-retaining property of matrix, can also be added in Nutrition Soil of the present invention 5~15% vermiculite it is (or precious
Zhu Yan).
Wherein, in above-mentioned golden Chinese scholartree cuttage and seedling culture method, Nutrition Soil described in step a first carries out desinsection before being overlying on seedbed and kills
Bacterium is handled.
Further, after the processing of Nutrition Soil pesticide-germicide is admixes Nutrition Soil using pesticide-germicide medicine described in step a, with modeling
Expect that film covers 3~5 days.
Further, the pesticide-germicide medicine is the mixture of phoxim emulsifiable concentrate and carbendazim.
Further, the pesticide-germicide medicament preparation is 50% 100~165mL of phoxim emulsifiable concentrate and carbendazim powder
10~15g of agent is watered 5~7.5kg and is prepared.50kg Nutrition Soils are added in the above-mentioned pesticide-germicide medicine prepared when use
In, it is uniformly mixed.
With 50% phoxim emulsifiable concentrate insecticide, matrix desinsection processing is carried out, the pest in matrix can be effectively killed, prevented
Only its injury to branch.Matrix sterilization processing is carried out with powder of carbendazim fungicide, can effectively kill having in matrix
It falls ill bacterium, prevents infection of the harmful levels of pathogens after branch cutting in matrix to branch, avoid branch base portion from rotting, raising survives
Rate.
Wherein, in above-mentioned golden Chinese scholartree cuttage and seedling culture method, the Nutrition Soil described in step a be covered in the thickness on seedbed be 10~
15cm.The excessively high increase production cost of the thickness of Nutrition Soil on seedbed, too low performance of keeping humidity reduce, influence to survive.
Wherein, in above-mentioned golden Chinese scholartree cuttage and seedling culture method, cuttings described in step b is cut into upper end flat mouth, the shape of lower end angle,
40~50 degree of angle angle.The branch inferior end notch surface of a wound is excessive, and infected probability is just big, crosses micro- water absorbing capacity of small stems lower end
Power is just poor, it is made to be cut into 40~50 degree of inclined-plane, that is, reduces being infected probability, and can guarantee micro- water absorbing capacity of branch lower end,
It is effective to improve survival rate.
Wherein, in above-mentioned golden Chinese scholartree cuttage and seedling culture method, cuttings disinfection described in step b uses 500~800 times of carbendazim solutions
Immersion 16~for 24 hours.
Wherein, in above-mentioned golden Chinese scholartree cuttage and seedling culture method, cuttings described in step b is oblique cutting, cuttings and matrix when being inserted into matrix
Plane forms 40~50 degree of angle.If oblique angle is excessive, cuttings tends to be vertical, and due to gravity, branch itself moisture is concentrated
Moisture in branch lower end, branch upper end is less, is easy to dry up, difficulty is caused to germination.If oblique angle is too small, cuttings tends to be flat
Row, the depth that branch is inserted into matrix is shallower, and water-retaining property is poor, is also unfavorable for surviving.So the oblique angle of 40~50 degree of selection,
The moisture for maintaining cuttings not easily runs off, and cuttings can be allowed to have certain depth in matrix, plays water retention, be conducive at
It is living.
Wherein, in above-mentioned golden Chinese scholartree cuttage and seedling culture method, 4~5cm of oblique cutting insertion depth described in step b, each cuttings interval 5
~6cm.
Inventor does not use fungicide and taking root liquid to handle cuttings when initially carrying out cuttage processing to golden Chinese scholartree,
Cuttage shoot survival percent is only capable of reaching 5% or so.
In order to improve cuttage shoot survival percent, inventor is considered as fungicide and taking root liquid and handles golden Chinese scholartree.But it is existing
There is the cuttage that technology does not disclose fungicide and whether taking root liquid can be used in golden Chinese scholartree, if golden Chinese scholartree cutting survival can be improved
Rate also there are no any suitable report using which kind of fungicide and taking root liquid on golden Chinese scholartree.And in plant cuttage nursery field,
The type of plant is different, and the cell activity of same root-growing agent stimulation branch incision is different, same plant, different root-growing agents
Stimulate the cell activity of branch incision also different.In addition, the dosage of different root-growing agents, and the time of processing are long
Short difference stimulates the cell activity of branch incision also different.For this purpose, inventor's in industry numerous fungicide and taking root liquid
In carried out screening combination, to improve the survival rate of golden Chinese scholartree cuttage seeding.Specific screening experiment is as follows:
Influence of one fungicide of screening experiment to cuttage survival rate
Processing group is as follows:Experiment is divided into 6 groups, and one of which is not use any fungicide, and it is to adopt that remaining five groups primary
Handled with thiophanate methyl, carbendazim, triadimefon, Bravo, thiophanate-methyl, each medicament be respectively adopted 300 times,
500 times, 700 times, 800 times, 1000 times, 1200 times of 6 concentration, obtain result as shown in table 1 below.
Influence of the different fungicide of table 1 to golden Chinese scholartree cuttage survival rate (%)
It is not used | Thiophanate methyl | Carbendazim | Triadimefon | Bravo | Thiophanate-methyl | |
300 times of liquid | 6% | 43% | 62% | 39% | 51% | 36% |
500 times of liquid | 6% | 51% | 78% | 42% | 59% | 39% |
700 times of liquid | 6% | 48% | 81% | 40% | 61% | 42% |
800 times of liquid | 6% | 55% | 79% | 46% | 54% | 45% |
1000 times of liquid | 6% | 46% | 64% | 35% | 46% | 33% |
1200 times of liquid | 6% | 41% | 62% | 32% | 44% | 31% |
It can be seen that by the result of table 1:In above-mentioned fungicide, when using the processing of 500~800 times of carbendazim, cuttage at
Motility rate highest.The standard of cutting survival of the present invention, which is branch base portion, has callus to be formed, and grows new root, branch upper end
The part for exposing matrix has sprouting to grow, and grows into shoot.
Influence of two taking root liquid of screening experiment to cuttage survival rate
In order to investigate whether taking root liquid influences the survival rate of cuttage seeding, inventor has carried out screening experiment two.
Inventor takes IAA (heteroauxin) and NAA (methyl α-naphthyl acetate) to test respectively.
Experiment is grouped as follows:
IAA groups;Concentration screening 0,500pmm, 800ppm, 1000ppm, 1200ppm, respectively handled 1s, 2s, 3s,
4s。
NAA groups:Concentration screening 0,200pmm, 300ppm, 500ppm, 700ppm, have handled 1s, 2s, 3s, 4s respectively.
Specific test result is as shown in the following table 2 and table 3:
Influences of 2 IAA of table to golden Chinese scholartree cuttage survival rate
Influences of 3 NAA of table to golden Chinese scholartree cuttage survival rate (%)
From the data of above-mentioned table 2 and table 3:Using the cutting survival of 2~3s of IAA solution treatments of 800~1000ppm
Rate higher.
Influence of the different cottage methods of screening experiment three to cuttage survival rate
The method progress cuttage of straight cutting, oblique cutting is respectively adopted, investigates influence of the different cottage methods to cuttage survival rate, has
The experimental design of body and result are as shown in table 4 below.
Influence of the different cottage method of table 4 to golden Chinese scholartree cuttage shoot survival percent (%)
As shown in Table 4:Using oblique cutting than the cottage method survival rate higher using straight cutting, especially when oblique cutting angle
Degree is:Effect is more preferable when 40~50 degree of cuttage seeding and seedbed angle, this is because the result of the effective moisture for maintaining branch.
Influence of the different cutting mediums of screening experiment four to cuttage survival rate
Matrix A:The Nutrition Soil that soil, turf and perlite are prepared by weight 50 ︰, 40 ︰ 10.
Matrix B:The Nutrition Soil that soil, turf and vermiculite are prepared by weight 45 ︰, 45 ︰ 10.
Matrix C:The Nutrition Soil that soil, turf and vermiculite are prepared by weight 30 ︰, 60 ︰ 10.
Matrix D:Common soil.
Influence of 5 different substrates of table to golden Chinese scholartree cuttage shoot survival percent (%)
Matrix A | Matrix B | Matrix C | Matrix D | |
Survival rate (%) | 80 | 79.5 | 71 | 42 |
From the data of table 5:Using soil, turf and perlite (or vermiculite) by weight 50:40:10 battalion prepared
Soil is supported, the survival rate of golden Chinese scholartree cuttage seeding significantly improves.
Explanation is further explained to the specific implementation mode of the present invention below in conjunction with embodiment, but is not indicated that this
The protection domain of invention is limited in range described in embodiment.
Golden Chinese scholartree cuttage branch used in embodiment and comparative example is planted selected from the Sichuan bio tech ltd Huai Jin gold Chinese scholartree
Base, the reagents such as described carbendazim are ordinary commercial products.
Embodiment 1 carries out cuttage and seedling culture using the method for the present invention to golden Chinese scholartree
It is as follows;
A, smooth cuttage seedbed prepares Nutrition Soil, Nutrition Soil is overlying on cuttage seedbed, forms cutting medium;The battalion
It is that soil, turf and vermiculite are formulated by weight 45 ︰, 45 ︰ 10 to support soil;
B, on the golden Chinese scholartree elite stand of robust growth clip without the annual golden Chinese scholartree branch of disease pest, be cut into 12cm long, upper end flat mouth,
The segment (shown in Fig. 1) of lower end angle, wisp is bundled into rubber ring;
The golden Chinese scholartree cuttings for being bundled into wisp is placed in 500 times of carbendazim solutions and is impregnated 20 hours, is pulled out after impregnating well with clear
Water rinses, and is positioned in polybag and keeps cuttings moistening;
IAA solution 1000ppm are prepared, after 2s is dipped in cuttings lower end, 45 ° of inclinations averagely insert in culture medium respectively, insert
Enter 4~5cm of depth, 5 × 6cm of spacing (shown in Fig. 2) is watered with moisture after being plugged;
C, it is managed after cuttage
It is 18 DEG C that air themperature is controlled after cuttage, air humidity 40%;In cuttage 40~50 days, control soil humidity is
70%, after cuttage 40~50 days, control soil humidity is 55%.
When golden Chinese scholartree seedling that embodiment cuttage obtains forms callus, when growing new root and the when of surviving is respectively such as Fig. 3, figure
4, shown in Fig. 5.
Embodiment 2 carries out cuttage and seedling culture using the method for the present invention to golden Chinese scholartree
It is as follows;
A, smooth cuttage seedbed prepares Nutrition Soil, Nutrition Soil is overlying on cuttage seedbed, forms cutting medium;The battalion
It is that soil, turf and perlite are formulated by weight 50 ︰, 40 ︰ 10 to support soil;
B, on the golden Chinese scholartree elite stand of robust growth clip without the annual golden Chinese scholartree branch of disease pest, be cut into 12cm long, upper end flat mouth,
The segment of lower end angle is bundled into wisp with rubber ring;
The golden Chinese scholartree cuttings for being bundled into wisp is placed in 500 times of carbendazim solutions and is impregnated 20 hours, is pulled out after impregnating well with clear
Water rinses, and is positioned in polybag and keeps cuttings moistening;
IAA solution 1000ppm are prepared, after 2s is dipped in cuttings lower end, 45 ° of inclinations averagely insert in culture medium respectively, insert
Enter 4~5cm of depth, 5 × 6cm of spacing is watered with moisture after being plugged;
C, it is managed after cuttage
It is 20 DEG C that air themperature is controlled after cuttage, air humidity 50%;In cuttage 40~50 days, control soil humidity is
70%, after cuttage 40~50 days, control soil humidity is 60%.
Embodiment 3 carries out cuttage and seedling culture using the method for the present invention to golden Chinese scholartree
It is as follows;
A, smooth cuttage seedbed prepares Nutrition Soil, Nutrition Soil is overlying on cuttage seedbed, forms cutting medium;The battalion
It is that soil, turf and vermiculite are formulated by weight 45 ︰, 40 ︰ 15 to support soil;
B, on the golden Chinese scholartree elite stand of robust growth clip without the annual golden Chinese scholartree branch of disease pest, be cut into 12cm long, upper end flat mouth,
The segment of lower end angle is bundled into wisp with rubber ring;
The golden Chinese scholartree cuttings for being bundled into wisp is placed in 500 times of carbendazim solutions and is impregnated 20 hours, is pulled out after impregnating well with clear
Water rinses, and is positioned in polybag and keeps cuttings moistening;
IAA solution 1000ppm are prepared, after 2s is dipped in cuttings lower end, 45 ° of inclinations averagely insert in culture medium respectively, insert
Enter 4~5cm of depth, 5 × 6cm of spacing is watered with moisture after being plugged;
C, it is managed after cuttage
It is 25 DEG C that air themperature is controlled after cuttage, air humidity 60%;In cuttage 40~50 days, control soil humidity is
75%, after cuttage 40~50 days, control soil humidity is 65%.
Comparative example 1 does not use the method for the present invention to carry out cuttage and seedling culture to golden Chinese scholartree
In comparative example 1, in addition to the soil humidity in step c is always 75%, remaining condition is the same as embodiment 1.
Comparative example 2 carries out cuttage and seedling culture using existing cottage method to golden Chinese scholartree
It is as follows:
A, smooth cuttage seedbed, using normal soil, it is desirable that soil is thin, without weeds, without stone;
B, clip is cut into the segment of 12cm long without the annual golden Chinese scholartree branch of disease pest on the golden Chinese scholartree elite stand of robust growth, point
For other straight cutting in culture medium, 4~5cm of insertion depth, 5 × 6cm of spacing are watered with moisture after being plugged;
C, it is managed after cuttage
It is 25 DEG C that air themperature is controlled after cuttage, air humidity 40%;In cuttage 40~50 days, control soil humidity is
70%, after cuttage 40~50 days, control soil humidity is 55%.
Golden each character of Chinese scholartree seedling that embodiment and comparative example cuttage obtains is as shown in table 6 below.
The golden Chinese scholartree seedling quality that 6 distinct methods cuttage of table obtains
By embodiment and comparative example it is found that using the method for the present invention, by controlling the composition of matrix, using suitable sterilization
Agent and taking root liquid are handled, and it is oblique cutting to adjust cuttage mode, suitable water supply in media is controlled after cuttage, above-mentioned steps are organic
Be combined into an entirety, jointly so that cuttage shoot survival percent be increased to 80% or so by 5%, have obviously imitate
Fruit achieves significant progress.
Claims (10)
1. gold medal Chinese scholartree cuttage and seedling culture method, which is characterized in that include the following steps:
A, smooth cuttage seedbed prepares Nutrition Soil, Nutrition Soil is overlying on cuttage seedbed, forms cutting medium;The Nutrition Soil
It is formulated by weight 45~50 ︰, 40~45 ︰ 5~15 for soil, turf and vermiculite;
B, healthy and strong annual golden Chinese scholartree branch is chosen, the cuttings of long 10~12cm is cut into, cuttings disinfection is placed on 800~1200ppm
IAA solution in dip in 2~3s after, cuttage is sprinkled profoundly water in matrix after cuttage;
C, it is 18~25 DEG C that air themperature is controlled after cuttage, and air humidity is 40~60%;In cuttage 40~50 days, matrix is controlled
Humidity is 70~75%, and after cuttage 40~50 days, control soil humidity is 55~65%.
2. gold Chinese scholartree cuttage and seedling culture method according to claim 1, it is characterised in that:Soil described in step a and turf mistake
80 mesh sieve.
3. gold Chinese scholartree cuttage and seedling culture method according to claim 1, it is characterised in that:Nutrition Soil described in step a is being overlying on
Pesticide-germicide processing is first carried out before seedbed.
4. gold Chinese scholartree cuttage and seedling culture method according to claim 1, it is characterised in that:Nutrition Soil pesticide-germicide described in step a
Processing is after admixing Nutrition Soil using pesticide-germicide medicine, with covered rearing with plastic film 3~5 days.
5. gold Chinese scholartree cuttage and seedling culture method according to claim 1, it is characterised in that:The pesticide-germicide medicine is phoxim
The mixture of missible oil and carbendazim.
6. gold Chinese scholartree cuttage and seedling culture method according to claim 1, it is characterised in that:The pesticide-germicide medicament preparation
For 50% 100~165mL of phoxim emulsifiable concentrate and 10~15g of powder of carbendazim, it is watered 5~7.5kg and is prepared.
7. gold Chinese scholartree cuttage and seedling culture method according to claim 1, it is characterised in that:Cuttings, which is cut into, described in step b holds level with both hands
The shape of mouth, lower end angle, 40~50 degree of angle angle.
8. gold Chinese scholartree cuttage and seedling culture method according to claim 1, it is characterised in that:Cuttings disinfection described in step b uses 500
~800 times of carbendazim solutions immersions 16~for 24 hours.
9. gold Chinese scholartree cuttage and seedling culture method according to claim 1, it is characterised in that:When cuttings described in step b is inserted into matrix
For oblique cutting, cuttings forms 40~50 degree of angle with substrate plane.
10. according to claim 1~9 any one of them gold Chinese scholartree cuttage and seedling culture method, it is characterised in that:Vermiculite described in step a
It is replaced by perlite.
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