CN109041847B - Method for rejuvenation and asexual propagation seedling culture of adult wingpod trees - Google Patents
Method for rejuvenation and asexual propagation seedling culture of adult wingpod trees Download PDFInfo
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- CN109041847B CN109041847B CN201811009998.6A CN201811009998A CN109041847B CN 109041847 B CN109041847 B CN 109041847B CN 201811009998 A CN201811009998 A CN 201811009998A CN 109041847 B CN109041847 B CN 109041847B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
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- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for rejuvenation and asexual propagation seedling of adult wingpod trees, which comprises the following steps: selecting a dry straight adult superior wingpod tree which is free from diseases and insect pests and strong in growth, digging a tree root, cutting the tree root into cutting slips with the growth of 10-12 cm, soaking the cutting slips in a potassium indolebutyrate solution, burying the cutting slips into a nursery, cutting the cutting slips when the sprouting branches of the root shoot seedlings grow to a semi-lignification degree, soaking the cutting slips in a potassium indolebutyrate solution, then cutting, and selecting cutting seedlings with a large number of roots for hardening, transplanting and afforesting. The method can solve the problems of crown-bias aging, low rooting rate and the like of the trunk used in the excellent clone breeding process of the wingpod wood, has the effects of rejuvenation and rejuvenation, and greatly improves the rooting rate of cuttage; can be propagated for a plurality of times in one year, has high propagation coefficient and large propagation scale, is more convenient and quicker than the prior seed propagation, and can rapidly propagate a large amount of seedlings of the winged pod wood.
Description
Technical Field
The invention belongs to the technical field of forest breeding, and particularly relates to a method for rejuvenation and asexual propagation seedling of adult wingpod trees.
Background
Winged pod wood (Zenia insignis Chun) belongs to sappanwood, belongs to deciduous trees, is mainly distributed in provinces (regions) such as Guizhou, Yue, Xiang, Qian, Dian and the like, is nearly extinct at present and belongs to key protection plants in China. The winged pod wood grows very fast, has extremely strong barren and drought resistance, and can grow up to 2.2cm at the annual average breast diameter and 1.5m at the annual average tree height under the condition of medium land. The wood is firm and dense in texture, and is valuable and excellent wood. The seed can be used for oil extraction, and can be used in paint, soap and other industries. The wood can be prevented from being damaged by worms after being soaked in water and dried in the shade, does not warp and crack, and can be used as a truss head, a door and window, a plate, furniture and the like. The leaves contain protein, the tender branches and leaves can be used as feed for cattle and sheep, and the leaves can be used as green manure and retted compost in the rice field. The flower is yellowish brown, is suitable for viewing, can secrete honeydew, and is a good viewing and honeydew source tree species. The wingpod wood integrates multiple functions and has wide development and utilization value prospect.
The wingpod wood is tall and big, the seeds are difficult to collect, and the individual differentiation of the seedlings propagated by the seeds is large, so that the way of walking the clone like eucalyptus and poplar is the necessary way for developing the artificial forest of the wingpod wood. However, practices prove that nursery stocks bred by cutting the middle-upper branches of the wingpod wood trunk have serious phenomena of crown deviation and growth decline, the wingpod wood tissue culture technology has not been broken through, the wingpod wood seeding and seedling raising can only be carried out for 1 time per year at present, the influence of germination rate and time season is caused, the tissue culture, grafting and conventional cutting propagation coefficients are quite low, and the development of the wingpod wood high-yield and high-efficiency breeding industry is severely restricted.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects and defects mentioned in the background technology, provide a method for rejuvenating adult wingpod trees and carrying out vegetative propagation seedling culture, can solve the problems of crown-bias aging, low rooting rate and the like of trunks used for excellent clonal breeding of the wingpod trees, and has the effects of rejuvenating and rejuvenating, and the rooting rate of cuttage is greatly improved.
In order to solve the technical problems, the technical scheme provided by the invention is as follows:
a method for rejuvenation and asexual propagation seedling raising of adult wingpod trees comprises the following steps:
(1) selecting a dry straight adult superior wingpod tree which is free from diseases and insect pests and grows robustly, digging a root at a position 30-50 cm away from a trunk, and taking the root as a parent plant for root tiller propagation;
(2) cutting the tree roots dug in the step (1) into cuttings with the length of 10-12 cm, soaking the cuttings in a potassium indolebutyrate (KIBA auxin) solution with the concentration of 190-200 ppm for 25-35 min, burying the cuttings in a nursery land, and waiting for the root shoots to germinate;
(3) when the sprouting branches of the tillering seedlings in the step (2) grow to a semi-lignification degree, selecting disease-free sprouting branches of the tillering seedlings to cut cutting slips, reserving 2-3 knots and 2 pairs of leaves of the cut cutting slips for 8-10 cm in length, disinfecting the cutting slips, and soaking the sterilized cutting slips in a potassium indolebutyrate (KIBA auxin) solution with the concentration of 390-400 ppm for 25-35 min;
(4) and (3) sterilizing a cutting substrate before cuttage, namely, inserting a small hole in the cutting substrate during cuttage, inserting the cutting slips obtained in the step (3) into the small hole, controlling the cuttage depth to be 2.5-3.5 cm, slightly pressing, spraying 1000 times of diluted chlorothalonil solution, thoroughly watering, placing in a greenhouse for rooting and growing, and selecting cutting seedlings with the root number of 4 or more for seedling hardening, transplanting and mountain-climbing afforestation.
According to the invention, the indole butyric acid potassium is used as auxin to soak the tree root and the cutting shoot, so that the cell division of the treated part can be promoted, and the growth is promoted. The potassium indolebutyrate has the characteristics of long-acting property and specificity, and can promote the growth of new roots, induce the formation of root origins and promote the formation of adventitious roots of cuttings.
In the above method, preferably, in the step (1), the diameter of the cross section of the dug tree root is more than 0.3cm, and the length of the cross section of the dug tree root is more than 10 cm.
Preferably, in the step (2), the upper and lower ends of the cutting are morphologically buried in the nursery.
Preferably, in the step (3), the cutting shoot is sterilized by using a chlorothalonil solution diluted by 1000 times.
Preferably, in the step (4), the cuttage matrix is sterilized by chlorothalonil or thiophanate methyl solution.
Preferably, in the step (4), the cutting medium is obtained by mixing peat soil and perlite according to a mass ratio of 2: 1.
Preferably, in the step (4), the sprayed chlorothalonil solution is a chlorothalonil solution with a mass concentration of 75%.
Preferably, in the step (4), during rooting growth in a greenhouse, water spraying is carried out for 8-10 s every 8-10 min.
Compared with the prior art, the invention has the beneficial effects that:
the method can solve the problems of partial crown aging and low rooting rate of the trunk used in the excellent clone breeding process of the winged podded wood, has the effects of rejuvenation and rejuvenation, can greatly improve the rooting rate of cuttage, can grow roots in about 25 days, can reach the rooting rate of 87% in 30 days, has the rooting number of more than 4, can more quickly achieve the effect of quick propagation of excellent varieties, can multiply propagate in one year, has high propagation coefficient and large propagation scale, is more convenient and quick than the prior seed propagation, can quickly propagate a large number of winged podded wood seedlings, is a means for protectively developing and utilizing winged podded wood plant resources, and can provide powerful technical support for the construction of artificial forest bases of the winged podded wood.
Detailed Description
In order to facilitate an understanding of the present invention, the present invention will be described more fully and in detail with reference to the preferred embodiments, but the scope of the present invention is not limited to the specific embodiments below.
Unless otherwise defined, all terms of art used hereinafter have the same meaning as commonly understood by one of ordinary skill in the art. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the present invention.
Unless otherwise specifically stated, various raw materials, reagents and the like used in the present invention are commercially available or can be prepared by existing methods.
Example (b):
the invention relates to a method for rejuvenating adult wingpod trees and performing vegetative propagation seedling culture, which comprises the following steps:
(1) selecting a superior tree and digging a root: selecting a dry straight adult superior wingpod tree which is free from diseases and insect pests and grows robustly, digging a tree root with the cross section diameter of more than 0.3cm and the length of more than 10cm at a position 30-50 cm away from a trunk, and taking the tree root as a parent plant for the propagation of root tillers;
(2) root burying treatment: cutting the tree roots dug in the step (1) into cutting slips with the length of 10-12 cm, soaking the cutting slips in a potassium indolebutyrate solution (KIBA auxin) with the concentration of 200ppm for 30min, burying the upper and lower ends of the soaked cutting slips into a nursery garden according to morphology, forming callus within about 10 days, beginning a large amount of root tillers to germinate within about 1 month, wherein the average number of germinating branches is more than 2, and the rooting germination rate is more than 86%;
(3) shearing the sprout strips: when the sprouting branches of the tillering seedlings in the step (2) grow to a semi-lignification degree, cutting the sprouting branches of the tillering seedlings without diseases into cutting slips, reserving 2-3 joints and 2 pairs of leaves of the cutting slips for 8-10 cm, disinfecting the cutting slips by using 1000 times of diluted chlorothalonil solution, and soaking the cutting slips in 400ppm indolylbutyrate potassium (KIBA auxin) solution for 30 min;
(4) cuttage: mixing peat soil and perlite according to a mass ratio of 2:1 to prepare a cutting matrix, filling the cutting matrix into a seedling raising plug tray with a 50-plug specification, sterilizing the cutting matrix by using chlorothalonil or thiophanate methyl solution before cutting, inserting a small hole in the cutting matrix during cutting, inserting the cutting slips obtained in the step (3) into the small hole, controlling the cutting depth to be about 3cm, slightly pressing the cutting slips, spraying 75% chlorothalonil solution (the mass concentration is 75%) diluted by 1000 times, and completely pouring the cutting slips;
(5) managing after the insertion: and (3) placing the seedling tray which is subjected to cuttage after the step (4) in a greenhouse for rooting and growing, spraying water for 10s every 10min, forming callus in about 10 days, beginning rooting in about 25 days, counting at 30 days, wherein the average rooting number is more than 4, the rooting rate reaches 87%, hardening seedlings, transplanting and mountain-climbing afforestation can be selected according to needs, the transplanting survival rate can reach more than 85%, the problems that the tree trunk is aged with partial crown and low rooting rate and the like in the excellent clone breeding process of the wingpod wood can be solved, the effects of rejuvenation and rejuvenation are achieved, the rooting rate of cuttage is greatly improved, and the effect of quick propagation of excellent varieties can be achieved more quickly.
The method can multiply propagate in one year, has high propagation coefficient and large propagation scale, is more convenient and quicker than the prior seed propagation, and can rapidly propagate a large amount of seedlings of the wingpod wood; preferably, selection of superior trees and root digging are carried out in spring, the time for cutting sprout is selected to be 5-9 months, and after the sprout enters 11 months, the young trees are not suitable for picking ears and raising seedlings because the wingpod wood enters a dormant period.
Claims (6)
1. A method for rejuvenation and asexual propagation seedling raising of adult wingpod trees comprises the following steps:
(1) selecting a dry straight adult superior wingpod tree which is free from diseases and insect pests and grows robustly, digging a tree root with the cross section diameter of more than 0.3cm and the length of more than 10cm at a position 30-50 cm away from a trunk, and taking the tree root as a parent plant for root tiller propagation;
(2) cutting the tree roots dug in the step (1) into cuttings with the length of 10-12 cm, soaking the cuttings in a potassium indolebutyrate solution with the concentration of 190-200 ppm for 25-35 min, burying the cuttings in a nursery land, and waiting for the tillering seedlings to germinate;
(3) when the sprouting branches of the tillering seedlings in the step (2) grow to a semi-lignification degree, selecting disease-free sprouting branches of the tillering seedlings to cut cutting slips, reserving 2-3 knots and 2 pairs of leaves of the cut cutting slips for 8-10 cm in length, disinfecting the cutting slips, and soaking the sterilized cutting slips in a potassium indolebutyrate solution with the concentration of 390-400 ppm for 25-35 min;
(4) sterilizing a cutting matrix before cuttage, wherein the cutting matrix is obtained by mixing peat soil and perlite according to a mass ratio of 2:1, a small hole is firstly inserted in the cutting matrix during cuttage, the cutting shoot obtained in the step (3) is inserted into the small hole, the cuttage depth is controlled to be 2.5-3.5 cm, the small hole is lightly pressed, then 1000 times of diluted chlorothalonil solution is sprayed, the hole is thoroughly watered, the hole is placed in a greenhouse for rooting growth, and cutting seedlings with the root number of 4 or more are selected for seedling hardening, transplanting and mountain-climbing afforestation.
2. The method according to claim 1, wherein in the step (2), the cutting is morphologically buried at the upper and lower ends in the nursery.
3. The method as claimed in claim 1, wherein in the step (3), the cutting disinfection is performed by using a chlorothalonil solution diluted 1000 times.
4. The method according to claim 1, wherein in the step (4), the cutting medium is sterilized by using chlorothalonil or thiophanate methyl solution.
5. The method as claimed in claim 1, wherein the chlorothalonil solution sprayed in step (4) is a chlorothalonil solution with a mass concentration of 75%.
6. The method according to claim 1, wherein in the step (4), during the rooting growth in the greenhouse, water is sprayed for 8-10 s every 8-10 min.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811009998.6A CN109041847B (en) | 2018-08-31 | 2018-08-31 | Method for rejuvenation and asexual propagation seedling culture of adult wingpod trees |
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| CN201811009998.6A CN109041847B (en) | 2018-08-31 | 2018-08-31 | Method for rejuvenation and asexual propagation seedling culture of adult wingpod trees |
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| CN109041847B true CN109041847B (en) | 2021-05-18 |
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| CN112136526A (en) * | 2020-09-29 | 2020-12-29 | 中南林业科技大学 | Greenhouse castanea henryi twig cutting seedling method |
| CN113141890B (en) * | 2021-03-03 | 2022-11-08 | 江西环境工程职业学院 | Method for promoting cutting rooting and survival of castanea mollissima tannin extract adult tree cutting slips |
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| CN103283447A (en) * | 2013-05-21 | 2013-09-11 | 江苏圣宏农业科技有限公司 | Full-light-break mist cutting and seedling culturing method for greenhouses |
| CN104429468B (en) * | 2014-11-07 | 2017-10-27 | 襄阳景源花木有限责任公司 | A kind of crape myrtle nursery stock cottage breeding method |
| CN106973746A (en) * | 2017-03-21 | 2017-07-25 | 浙江省林业科学研究院 | A kind of method of sylvan life pseudo-wild cultivating bar leaf banyan |
| CN108419552A (en) * | 2018-06-07 | 2018-08-21 | 福建省泉州罗溪国有林场 | A kind of black wooden yearning between lovers root turion cuttage and seedling culture method |
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