CN106613689B - Method for rapidly breeding paeonia suffruticosa - Google Patents
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- CN106613689B CN106613689B CN201611220146.2A CN201611220146A CN106613689B CN 106613689 B CN106613689 B CN 106613689B CN 201611220146 A CN201611220146 A CN 201611220146A CN 106613689 B CN106613689 B CN 106613689B
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- 240000005001 Paeonia suffruticosa Species 0.000 title claims abstract description 35
- 235000003889 Paeonia suffruticosa Nutrition 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 27
- 238000009395 breeding Methods 0.000 title claims abstract description 13
- 230000001488 breeding effect Effects 0.000 title claims abstract description 11
- 206010052428 Wound Diseases 0.000 claims abstract description 8
- 208000027418 Wounds and injury Diseases 0.000 claims abstract description 8
- 241000196324 Embryophyta Species 0.000 claims description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 239000002689 soil Substances 0.000 claims description 5
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims description 4
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 4
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 4
- 239000006013 carbendazim Substances 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 239000004021 humic acid Substances 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 239000004563 wettable powder Substances 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 3
- 239000000758 substrate Substances 0.000 claims description 2
- 238000003973 irrigation Methods 0.000 claims 2
- 230000002262 irrigation Effects 0.000 claims 2
- 230000035784 germination Effects 0.000 abstract description 7
- 241000736199 Paeonia Species 0.000 description 28
- 235000006484 Paeonia officinalis Nutrition 0.000 description 25
- 230000005059 dormancy Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000000249 desinfective effect Effects 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 241000124495 Paeonia delavayi Species 0.000 description 2
- 244000236658 Paeonia lactiflora Species 0.000 description 2
- 235000008598 Paeonia lactiflora Nutrition 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 102100020720 Calcium channel flower homolog Human genes 0.000 description 1
- 101000932468 Homo sapiens Calcium channel flower homolog Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 101150062587 flower gene Proteins 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for rapid breeding of paeonia suffruticosa, wherein sprouts sent from lateral roots are dug from the roots of the paeonia suffruticosa from the bottom of 9 months to the beginning of 11 months, the sprouts are required to be more than 5cm, the roots need to be kept for 8-15 cm, the wounds are leveled by scissors, cleaned and disinfected, all leaves and terminal buds are removed, the paeonia suffruticosa is placed in a shade place to air dry the wounds, and then the paeonia suffruticosa is planted in a nursery. The method provided by the invention is simple to operate, is suitable for seedling culture in a field environment, has the advantages of quick rooting and sprouting, high rooting rate and high germination rate, and greatly reduces the culture cost of the paeonia suffruticosa.
Description
Technical Field
The invention relates to the technical field of promoting the breeding of paeonia suffruticosa, in particular to a rapid breeding method of paeonia suffruticosa.
Background
Paeonia delavayi (Paeonia delavayi) is a special species in the southwest region of China, is a peony group with the soutest distribution of Paeonia, has important scientific research value on the origin, evolution and geographical distribution research of Paeonia plants, and becomes an important material for peony breeding because of the unique yellow flower gene thereof. Seed propagation is the most common propagation mode of peony, seedlings obtained by sowing have larger variation, more developed root systems and strong stress resistance, and the seedlings are the best materials for breeding new varieties and can provide rich diversity for selection; can also be used as grafting stock. However, the paeonia suffruticosa seeds are susceptible to diseases and insect pests under natural conditions, and the currently artificially cultivated paeonia suffruticosa cannot provide seed quantity for large-scale seedling culture; meanwhile, because the seed coat of the paeonia suffruticosa seed is hard, the paeonia suffruticosa seed has the characteristics of physiological after-ripening, upper and lower embryo dormancy and the like, the paeonia suffruticosa seed takes a long time for germination under natural conditions and strict climatic conditions, and the emergence rate of the paeonia suffruticosa seed is low.
About there is 10 seeds in yunnan peony's whole-length fruit pod, and the seed granule that is located in the middle of the whole-length fruit pod is comparatively plump, and the seed at both ends is less, and the seed at pod both ends does not sprout in one year after the seeding usually, and in addition there is the insect pest in the yunnan peony seed, if do not strictly screen, yunnan peony seed germination percentage in the first year is about 30%, and the germination percentage in the second year rises to 40 ~ 50%. And if only the intermediate seeds in the Yunnan peony seed clamp are adopted, the germination rate can be improved to about 70 percent, but the screening is difficult, and the cost is greatly increased.
Liu Xiuxian et al studied the effects of different temperatures and pretreatments on dormancy breaking of the hypocotyl of Paeonia suffruticosa seeds and on dormancy breaking of the epicotyl by different root lengths, temperatures and GA3 concentrations in the study of dormancy breaking effects of Paeonia suffruticosa seeds. The results show that: the paeonia suffruticosa seeds are soaked in 400mg/L GA3 solution for 24 hours and then have good effect of relieving the dormancy of hypocotyl at 15 ℃; the rooting seeds with the root length of more than 3cm are soaked in 200mg/L GA3 solution for 2h, and the dormancy of epicotyl of the paeonia suffruticosa seeds is most favorably released at the temperature of 20 ℃. Although hypocotyl dormancy can be relieved in low temperature treatment, the required time is longer, the effect is relatively poor, because this method must stimulate Yunnan peony rooting earlier, treat that the root length can only carry out the stimulation of sprouting after certain length, so this method only is applicable to the laboratory environment, be difficult to apply to in the nursery environment of tens of mu even hundreds of mu, and only adopt the seed at Yunnan peony whole-length fruit pod middle part, need pass through the heavy screening, and per ton ordinary Yunnan peony seed exceeds 20 ten thousand, if pass through the heavy screening, then increased planting cost greatly, so be not suitable for the field planting.
The cost for planting the peonies by adopting the seeds is too high, and the period is long, so that a breeding method which does not depend on the peonies is needed to be researched.
Disclosure of Invention
Aiming at the defects of the prior art, the invention discloses a method for rapidly breeding paeonia suffruticosa, wherein the method adopts bud seedlings emitted by lateral roots to breed, so that the rooting and germination time of the paeonia suffruticosa can be greatly shortened, the rooting rate and the germination rate are obviously improved, and the cost is saved.
The technical scheme of the invention is as follows: a method for quickly breeding Yunnan peony includes such steps as digging the bud seedlings from side roots from the root of Yunnan peony from the end of 9-11 months, cutting by 8-15 cm, smoothing the wound, washing, disinfecting, removing all leaves and terminal buds, drying in air, and planting in nursery.
Since the sprouts from the lateral roots are small and close to the surface of the earth, they are generally inhibited during growth and gradually shrink, and few sprouts exceed 15 cm.
Preferably, the sprouts are harvested by 5cm or more (the length of the sprouts includes the fleshy stem of the underground part and the sprouts of the above ground part).
The method for disinfecting the bud seedlings comprises the following steps: soaking 12.5% carbendazim wettable powder in 300-400 times of liquid for 15-20 min for disinfection, and then airing in the shade for later use.
Further, plant ash and raw soil are adopted after the bud seedlings are disinfected, wherein the ratio of the plant ash to the raw soil is 1: 3-5 as a substrate, mixing with 40mg/L IBA or rooting powder solution, wrapping the sterilized root of the bud seedling (which needs to be wrapped completely), and transplanting the bud seedling to a nursery.
And irrigating roots of the buds with 0.3-0.5% of humic acid at intervals of 30-35 days after the buds are planted in a nursery, and irrigating the roots for 1 time every 12-18 days, wherein the roots need to be irrigated for 3-5 times.
Preferably, the Yunnan peony is a plant with the growth of 3 years or more.
The mechanism of the invention is as follows: the paeonia suffruticosa belongs to a peony group fleshy flower disc subgroup plant, and the petal and the root of the paeonia suffruticosa are fleshy roots. In the process of growing the paeonia suffruticosa, the number of lateral roots is relatively large, and the positions of the lateral roots are relatively close to the ground surface; the stems of the peony seedlings are buried underground to grow plump roots, lateral roots of the peony seedlings can sprout seedlings in the growth process, however, because the seedlings are too small, nutrition and illumination do not compete with main branch Yunnan peony leaves, the seedlings from the lateral roots rarely survive for more than one year in the growth process (see figure 1), cultivators cannot think that the roots and the stems buried underground are adopted to sprout the seedlings to cultivate peony plants, and the importance of surface sprouting seedlings is ignored. Other peony varieties or white peony roots are new sprouts growing from main roots, and lateral roots are deep and basically have no sprouts growing from the lateral roots, so other varieties of peony or white peony roots are usually propagated in a way of division or grafting. However, the division of the plants can seriously affect the growth of the mother plants of the peonies, and the grafting survival rate is low, so the two methods are not suitable for the paeonia suffruticosa.
The method comprises the steps of collecting bud seedlings from the lateral roots of more than three years of paeonia suffruticosa at the bottom of 9-11 months, reserving 8-15 cm lateral roots in the bud seedling collection process, flattening wounds by using scissors, cleaning, disinfecting, removing all leaves and terminal buds, reserving fleshy stems only after the leaves and the terminal buds are completely removed, placing in a shade place to enable the wounds to be air-dried, wrapping by using a matrix, planting in a nursery garden, and performing field management by referring to the existing method of paeonia suffruticosa. Because the peony roots in winter, the bud seedlings grow fibrous roots about 1 month, and the survival rate is up to more than 90%. The method is simple, greatly reduces the planting cost, has high survival rate, and is particularly suitable for growing in high-altitude areas.
Compared with the prior art, the invention has the following beneficial effects: the method provided by the invention is simple to operate, is suitable for seedling culture in a field environment, has quick rooting and higher rooting rate, and greatly reduces the cultivation cost of the paeonia suffruticosa.
Drawings
FIG. 1 is a schematic diagram of the germination of lateral roots of Paeonia suffruticosa;
the labels in the figure are: 1-main root, 2-lateral root and 3-bud seedling.
Detailed Description
The technical solution of the present invention will be described in further detail with reference to specific examples, but the present invention is not limited to the following technical solutions.
Example 1
The method comprises the following steps of digging a bud seedling sent from a lateral root from the root of the Yunnan peony growing for more than 3 years at the bottom of 9 months, wherein the length of the bud seedling is 5-10 cm, the root of the bud seedling needs to be kept for 8-15 cm, the wound is leveled by using scissors, after cleaning, the 300-time liquid of 12.5% carbendazim wettable powder is adopted to be soaked for 15-20 min for disinfection, all leaves and terminal buds are removed, and the shady and cool place is dried for standby use to plant ash and raw soil 1: 4 as matrix, mixing with 40mg/L IBA or rooting powder solution, wrapping the sterilized root of sprout, and transplanting into nursery. After the seedlings are planted in the nursery, 0.4 percent humic acid is used for irrigating roots at intervals of 30 days, and then the roots are irrigated for 1 time at intervals of 12 days and 3 times. 1000 plants are planted, and the rooting starts in about one month, and the rooting rate is 93%.
Example 2
The method comprises the following steps of digging buds sent from side roots from the root of Yunnan peony growing for more than 3 years at the bottom of 10 months, wherein the length of the buds is 5-10 cm, the root of the buds needs to be kept for 8-15 cm, the wounds are leveled by using scissors, after cleaning, 400-time liquid of 12.5% carbendazim wettable powder is adopted for soaking for 15-20 min for disinfection, all leaves and terminal buds are removed, and the buds are dried in shade for later use and are planted with plant ash and raw soil 1: 4 as matrix, mixing with 40mg/L IBA or rooting powder solution, wrapping the sterilized root of sprout, and transplanting into nursery. After the bud seedlings are planted in the nursery garden, 0.5% humic acid is used for irrigating roots at intervals of 35 days, and then the roots are irrigated for 1 time every 15 days and need to be irrigated for 3 times. 5000 plants are planted, and the rooting starts in about one month, and the rooting rate is 95%.
Claims (4)
1. A method for rapid breeding of Paeonia suffruticosa is characterized in that bud seedlings from lateral roots are dug from the roots of Paeonia suffruticosa from the bottom of 9 to 11 months, the roots are kept for 8-15 cm, the wounds are leveled by using scissors, cleaning and disinfection are carried out, all leaves and terminal buds are removed, the cut is placed in a shade place to be air-dried, and then the cut is planted in a nursery garden;
the paeonia suffruticosa is a plant which grows for 3 years or more;
the length of the bud seedlings from the lateral roots is more than 5cm when the bud seedlings are dug.
2. The method for rapid breeding of paeonia suffruticosa as claimed in claim 1, wherein the disinfection method of the sprouts is as follows: soaking 12.5% carbendazim wettable powder in 300-400 times of liquid for 15-20 min for disinfection, removing all leaves and terminal buds, and airing in the shade for later use.
3. The method for rapid breeding of paeonia suffruticosa as claimed in claim 1, wherein the bud seedlings are treated by the following method after being sterilized: plant ash and raw soil 1: 3-5 as a substrate, mixing with 40mg/L IBA or rooting powder solution, wrapping the sterilized root of the bud seedling, and transplanting the bud seedling into a nursery.
4. The method for rapid propagation of Paeonia suffruticosa as claimed in claim 1, wherein the root irrigation of 0.3% -0.5% humic acid is performed 30-35 days after the sprout is planted in the nursery, and then the root irrigation is performed 1 time every 12-18 days, 3-5 times.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611220146.2A CN106613689B (en) | 2016-12-26 | 2016-12-26 | Method for rapidly breeding paeonia suffruticosa |
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| CN201611220146.2A CN106613689B (en) | 2016-12-26 | 2016-12-26 | Method for rapidly breeding paeonia suffruticosa |
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| CN106613689B true CN106613689B (en) | 2020-03-31 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114617018B (en) * | 2022-03-28 | 2023-08-08 | 云南省农业科学院高山经济植物研究所 | Method for ecological restoration of high-altitude mountain area by directly seeding Yunnan peony seeds |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101473741A (en) * | 2009-02-13 | 2009-07-08 | 兰州牡丹园艺开发公司 | Graft and rapid propagation method for commercial production of Paeonia rockii |
| CN105075863A (en) * | 2015-08-10 | 2015-11-25 | 甘肃农业大学 | Rapid paeonia rockii reproduction method |
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2016
- 2016-12-26 CN CN201611220146.2A patent/CN106613689B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101473741A (en) * | 2009-02-13 | 2009-07-08 | 兰州牡丹园艺开发公司 | Graft and rapid propagation method for commercial production of Paeonia rockii |
| CN105075863A (en) * | 2015-08-10 | 2015-11-25 | 甘肃农业大学 | Rapid paeonia rockii reproduction method |
Non-Patent Citations (2)
| Title |
|---|
| 中国野生牡丹自然繁殖特性研究;成仿云等;《园艺学报》;19970526;第24卷(第2期);180-184页 * |
| 牡丹繁殖技术;王峰等;《现代农业科技》;20110320(第6期);第219页 * |
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