CN109041847A - A kind of method of Common zenia bearing tree childrenization rejuvenation and asexual multiplication seedling - Google Patents

A kind of method of Common zenia bearing tree childrenization rejuvenation and asexual multiplication seedling Download PDF

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Publication number
CN109041847A
CN109041847A CN201811009998.6A CN201811009998A CN109041847A CN 109041847 A CN109041847 A CN 109041847A CN 201811009998 A CN201811009998 A CN 201811009998A CN 109041847 A CN109041847 A CN 109041847A
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China
Prior art keywords
root
cuttings
tree
seedling
cuttage
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CN201811009998.6A
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Chinese (zh)
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CN109041847B (en
Inventor
童方平
李贵
刘振华
陈瑞
吴敏
王栋
言倜信
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Hunan Academy of Forestry
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Hunan Academy of Forestry
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum

Abstract

The invention discloses a kind of Common zenia bearing tree childrenization rejuvenation and the methods of asexual multiplication seedling, include the following steps: that the Common zenia of dry-shaped straight, no disease and pests harm and robust growth adult select tree is selected to take tree root, tree root is cut into the cuttings of a length of 10~12cm, it is placed in indolebutyric acid potassium solution and impregnates, then it is embedded to nursery lot, clip cuttings when root turion seedling germinating branch being waited to grow to semi-lignified degree, it is placed in indolebutyric acid potassium solution and impregnates, then cuttage is carried out, selection, which is taken root, to be counted more cuttage seeding progress hardenings, transplants, afforestation of going up a hill.Method of the invention, can solve in Common zenia choiceness Breeding Process has the problems such as hat aging partially, rooting rate is low using trunk, has the function of childrenization, rejuvenation, and cutting plantation greatly improves;It can repeatedly breed within 1 year, breeding coefficient is high, and breeding scale is big, and it is easily and efficiently more than current seminal propagation, a large amount of Common zenia nursery stocks can be bred rapidly.

Description

A kind of method of Common zenia bearing tree childrenization rejuvenation and asexual multiplication seedling
Technical field
The invention belongs to forest raising technology field more particularly to a kind of Common zenia bearing tree childrenization rejuvenation and vegetative propagation The method of nursery.
Background technique
Common zenia (Zenia insignis Chun) is Caesalpiniaceae, belong to deciduous tree, be mainly distributed on osmanthus, Guangdong, Hunan, Guizhou Province, Yunnan etc. saves (area), endangered, belongs to National Wild Conservative Plants.Common zenia grows very rapid, barren-resistant and arid energy Power extra-heavy, average annual breast diameter growth amount is up to 2.2cm under medium land occupation condition, and average annual height increment is up to 1.5m.Timber is very Solid, texture is dense, is a kind of of great value superior wood.Seed can extract oil, for painting, the industry such as soap.Timber After bubble dries in the shade, it can keep away and damage by worms, not stick up and do not split, purlin head, door and window, plate, furniture etc. can be made.Leaf contains protein, browse Nursing cattle and sheep capable of using as feed, blade can make the green manure and retting compost in rice field.Flower yellowish-brown is suitable for watching, and can secrete honeydew, For good ornamental and nectar source tree species.Common zenia integrates multiple functions, and value of exploiting and utilizing has a extensive future.
Common zenia tree body is tall and big, seed collection is more difficult, and the nursery stock individual differentiation of seminal propagation is big, therefore as eucalyptus, poplar The road for walking clone is the only way for developing Common zenia artificial forest.But it was verified that using Common zenia trunk middle and upper part branch The nursery stock that cuttage is bred as has more serious inclined hat and growth decay, and Common zenia tissue culture technology not yet makes a breakthrough, and mesh Preceding Common zenia seeding and seedling raising every year can only be 1 time, and is influenced by germination percentage, season time, tissue culture, grafting, normal cutting propagation breeding Coefficient is at a fairly low, seriously constrains the development that Common zenia high-yield and high-efficiency cultivates industry.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the shortcomings of to mention in background above technology and defect, provide one The method of kind Common zenia bearing tree childrenization rejuvenation and asexual multiplication seedling can solve Common zenia choiceness breeding and use tree It is dry to have the problems such as hat aging partially, rooting rate is low, have effects that childrenization, rejuvenation, and cutting plantation greatly improves.
In order to solve the above technical problems, technical solution proposed by the present invention are as follows:
A kind of method of Common zenia bearing tree childrenization rejuvenation and asexual multiplication seedling, includes the following steps:
(1) Common zenia of dry-shaped straight, no disease and pests harm and robust growth adult select tree is selected, at from 30~50cm of trunk Take tree root, the maternal plant as root turion breeding;
(2) tree root that the step (1) takes is cut into the cuttings of a length of 10~12cm, be placed in concentration be 190~ 25~35min is impregnated in butyric acid potassium (KIBA auxin) solution of 200ppm, is then embedded to nursery lot, and root turion seedling is waited to sprout Hair;
(3) when the root turion seedling germinating branch after the step (2) grows to semi-lignified degree, disease-free root turion is selected Seedling germinates branch clip cuttings, and the cuttings of clip stays 2~3 sections and 2 pairs of blades, and cuttings disinfection is placed on dense by long 8~10cm Degree is that 25~35min is impregnated in butyric acid potassium (KIBA auxin) solution of 390~400ppm;
(4) cutting medium is carried out disinfection processing before cuttage, when cuttage first inserts duck eye on cutting medium, by the step Suddenly in the cuttings insertion duck eye obtained after (3), cuttage is deep-controlled gently to be compressed in 2.5~3.5cm, then sprays 1000 times Diluted Bravo solution, irrigates, and is placed in greenhouse and carries out growth of taking root, and selects cuttage of the number at 4 or more of taking root Seedling carries out hardening, transplants, afforestation of going up a hill.
The present invention impregnates tree root and cuttings as auximone using butyric acid potassium, can promote the cell for the treatment of site Division promotes growth.Butyric acid potassium has the characteristics that long-term effect and specificity, can promote new root, induces root body It is formed, promotes cutting Adventitious root initiation.
Above-mentioned method, it is preferred that in the step (1), the tree root cross-sectional diameter 0.3cm or more taken, long 10cm More than.
Preferably, in the step (2), cuttings is embedded to nursery lot according to morphology upper and lower side.
Preferably, in the step (3), cuttings disinfection is carried out disinfection with 1000 times of diluted Bravo solution.
Preferably, in the step (4), cutting medium is carried out disinfection processing using Bravo or thiophanate methyl solution.
Preferably, in the step (4), cutting medium is mixed to get by peat soil and perlite by the mass ratio of 2:1.
Preferably, in the step (4), the Bravo solution of sprinkling is the Bravo solution that mass concentration is 75%.
Preferably, it in the step (4), is placed in greenhouse and carries out growth period of taking root, every 8~10min carries out water spray 8~10s of mist.
Compared with prior art, the invention has the benefit that
Method of the invention, can solve in Common zenia choiceness Breeding Process has hat aging, life partially using trunk The problems such as root rate is low has the function of childrenization, rejuvenation, and cutting plantation greatly improves, and can go out within 25 days or so root, and 30 is born For root rate up to 87%, number of taking root reaches 4 or more, can reach excellent variety numerous effect fastly faster, and 1 year can repeatedly breed, numerous Coefficient height is grown, breeding scale is big, and it is easily and efficiently more than current seminal propagation, a large amount of Common zenia nursery stocks can be bred rapidly, be A kind of protective development utilizes a kind of means of Common zenia plant resources, can also build and be provided effectively for Common zenia Plantation base Technical guarantee.
Specific embodiment
To facilitate the understanding of the present invention, the present invention is done below in conjunction with preferred embodiment and more comprehensively, is meticulously described, But protection scope of the present invention is not limited to following specific embodiments.
Unless otherwise defined, all technical terms used hereinafter are generally understood meaning phase with those skilled in the art Together.Technical term used herein is intended merely to the purpose of description specific embodiment, and it is of the invention to be not intended to limitation Protection scope.
Unless otherwise specified, various raw material, the reagent etc. used in the present invention can be commercially available by market Or it can be prepared by existing method.
Embodiment:
A kind of method of Common zenia bearing tree childrenization rejuvenation and asexual multiplication seedling of the invention, includes the following steps:
(1) it select superior tree and uproots: in selecting the Common zenia of dry-shaped straight, no disease and pests harm and robust growth to grow up select tree, The tree root for taking cross-sectional diameter 0.3cm or more, long 10cm or more at from 30~50cm of trunk, the mother as root turion breeding Strain;
(2) it buries root processing: the tree root that step (1) takes being cut into the cuttings of a length of 10~12cm, being placed in concentration is 30min is impregnated in the indolebutyric acid potassium solution (KIBA auxin) of 200ppm, the cuttings after immersion is buried according to morphology upper and lower side Enter nursery lot, 10 days or so formation callus, 1 month or so root turion seedling starts largely to sprout, averagely 2 plants of branch number of germinating with On, bury 86% or more root germinating rate;
(3) it coppice shoot clip: when the root turion seedling germinating branch after step (2) grows to semi-lignified degree, selects disease-free Root turion seedling germinate branch clip cuttings, the cuttings of clip stays 2~3 sections and 2 pairs of blades, long 8~10cm, by cuttings with 1000 Diluted Bravo solution carries out disinfection to be placed in butyric acid potassium (KIBA auxin) solution that concentration is 400ppm and soak again Steep 30min;
(4) cuttage: peat soil and perlite are mixed with cutting medium by the mass ratio of 2:1, are packed into 50 cave specifications Seedling culture hole plate is carried out disinfection processing before cuttage with Bravo or thiophanate methyl solution, and when cuttage first inserts on cutting medium The cuttings obtained after step (3) is inserted into duck eye by duck eye, and cuttage is deep-controlled gently to be compressed in 3cm or so, is then sprayed 1000 times of diluted 75% Bravo solution (mass concentration 75%), irrigate;
(5) manage after inserting: by step (4), the good seedlings nursing plate of cuttage is placed in greenhouse and carries out growth of taking root afterwards, often 10min carries out water spray 10s, and 10 days or so formation callus start to take root for 25 days or so, and 30 days whens count, and averagely takes root For number at 4 or more, rooting rate reaches 87%, can select hardening as needed, transplant, afforestation of going up a hill, transplanting survival rate can reach 85% or more, can solve in Common zenia choiceness Breeding Process has the problems such as hat aging partially, rooting rate is low using trunk, Have the function of childrenization, rejuvenation, and cutting plantation greatly improves, excellent variety numerous effect fastly can be reached faster.
Of the invention method 1 year can repeatedly be bred, and breeding coefficient is high, and breeding scale is big, than current seminal propagation side Just, efficiently more, a large amount of Common zenia nursery stocks can be bred rapidly;Preferably, select superior tree and uprooting is advisable in spring, coppice shoot 5~September of selection of time of clip, into November after enter dormant period because of Common zenia, fringe nursery should not be adopted again.

Claims (8)

1. a kind of method of Common zenia bearing tree childrenization rejuvenation and asexual multiplication seedling, includes the following steps:
(1) Common zenia of dry-shaped straight, no disease and pests harm and robust growth adult select tree is selected, is taken at from 30~50cm of trunk Tree root, the maternal plant as root turion breeding;
(2) tree root that the step (1) takes is cut into the cuttings of a length of 10~12cm, being placed in concentration is 190~200ppm's 25~35min is impregnated in indolebutyric acid potassium solution, is then embedded to nursery lot, and root turion seedling is waited to sprout;
(3) when the root turion seedling germinating branch after the step (2) grows to semi-lignified degree, disease-free root turion seedling is selected to sprout Raw branch clip cuttings, the cuttings of clip stay 2~3 sections and 2 pairs of blades, and long 8~10cm, cuttings disinfection, which is placed on concentration, is 25~35min is impregnated in the indolebutyric acid potassium solution of 390~400ppm;
(4) cutting medium is carried out disinfection processing before cuttage, when cuttage first inserts duck eye on cutting medium, by the step (3) in the cuttings insertion duck eye obtained after, cuttage is deep-controlled gently to be compressed in 2.5~3.5cm, and then 1000 times of sprinkling is dilute The Bravo solution released, irrigates, and is placed in greenhouse and carries out growth of taking root, and selects cuttage seeding of the number at 4 or more of taking root It carries out hardening, transplant, afforestation of going up a hill.
2. the method according to claim 1, wherein in the step (1), the tree root cross-sectional diameter that takes 0.3cm or more, long 10cm or more.
3. the method according to claim 1, wherein cuttings is buried according to morphology upper and lower side in the step (2) Enter nursery lot.
4. the method according to claim 1, wherein cuttings disinfection is diluted with 1000 times in the step (3) Bravo solution carries out disinfection.
5. the method according to claim 1, wherein cutting medium uses Bravo or first in the step (4) Base thiophanate solution carries out disinfection processing.
6. the method according to claim 1, wherein cutting medium is by peat soil and pearl in the step (4) Rock is mixed to get by the mass ratio of 2:1.
7. the method according to claim 1, wherein the Bravo solution of sprinkling is quality in the step (4) The Bravo solution that concentration is 75%.
8. the method according to claim 1, wherein being placed in greenhouse in the step (4) and carrying out life of taking root During length, every 8~10min carries out 8~10s of water spray.
CN201811009998.6A 2018-08-31 2018-08-31 Method for rejuvenation and asexual propagation seedling culture of adult wingpod trees Expired - Fee Related CN109041847B (en)

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CN201811009998.6A CN109041847B (en) 2018-08-31 2018-08-31 Method for rejuvenation and asexual propagation seedling culture of adult wingpod trees

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CN112136526A (en) * 2020-09-29 2020-12-29 中南林业科技大学 Greenhouse castanea henryi twig cutting seedling method
CN113141890A (en) * 2021-03-03 2021-07-23 江西环境工程职业学院 Method for promoting cutting rooting and survival of castanea mollissima tannin extract adult tree cutting slips

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112136526A (en) * 2020-09-29 2020-12-29 中南林业科技大学 Greenhouse castanea henryi twig cutting seedling method
CN113141890A (en) * 2021-03-03 2021-07-23 江西环境工程职业学院 Method for promoting cutting rooting and survival of castanea mollissima tannin extract adult tree cutting slips

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